klhl12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	klhl12
Entrez Gene ID	492362
Full Name	kelch-like family member 12
Synonyms	wu:fi60g03,zgc:92570
General protein information	Preferred Names kelch-like family member 12 Names kelch-like protein 12 kelch-like 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007328.1	NP_001007329.1	kelch-like protein 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	KLHL12	NP_067646.1
Canis lupus familiaris (dog)	KLHL12	XP_537119.2
Gallus gallus (chicken)	LOC101748977	XP_004934891.1
Pan troglodytes (chimpanzee)	KLHL12	XP_514106.3
Rattus norvegicus (Norway rat)	Klhl12	NP_714952.1
Danio rerio (zebrafish)	klhl12	NP_001007329.1
Bos taurus (cattle)	KLHL12	NP_001070593.1
	KLHL12	XP_002802018.1
Mus musculus (house mouse)	Klhl12	NP_694768.1
Xenopus tropicalis (tropical clawed frog)	klhl12	NP_001106569.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Saltatory evolution of the ectodermal neural cortex gene family at the vertebrate origin.
Feiner N, Murakami Y, Breithut L, Mazan S, Meyer A, Kuraku S
Genome biology and evolution5(8)1485-502(2013)

Adiponectin and adiponectin receptor genes are coexpressed during zebrafish embryogenesis and regulated by food deprivation.
Nishio S, Gibert Y, Bernard L, Brunet F, Triqueneaux G, Laudet V
Developmental dynamics : an official publication of the American Association of Anatomists237(6)1682-90(2008 Jun)

The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss MJ, Moon RT
Nature cell biology8(4)348-57(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following klhl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klhl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02727	NM_001007328.1 Latest version!	Danio rerio kelch-like family member 12 (klhl12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klhl12 ( NM_001007328.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007329.1 Danio rerio kelch-like family member 12 (klhl12), mRNA.

Price & Availability↑

CloneID	ODa02727
Clone ID Related Accession (Same CDS sequence)	NM_001007328.1
Accession Version	NM_001007328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Danio rerio(zebrafish)
Product	kelch-like protein 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085673.1. On Jul 6, 2005 this sequence version replaced XM_689517.1. ##Evidence-Data-START## Transcript exon combination :: BC085673.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGCCCA AGGACATCAT GACCAATTCT CATGCCAAAT CCATCTTGAA TGCCATGAAC 
GCCCTGAGAA AGAGCAACAC GCTGTGTGAC ATCACTCTGA GGGTGGAGGG CACAGATTTT 
CCTGCCCACC GCATTGTGTT GGCGGCGTGC AGTGATTATT TCTGCGCCAT GTTCACCAGT 
GAGCTTGCTG AAAAAGGGAA GTCCTTTGTG GACATTCAGG GCCTCACAGC GTCCACTATG 
GAGATCCTGC TGGATTTTGT TTACACAGAG ACAGTGCTGG TCACGGTAGA AAACGTCCAG 
GAGCTGCTGC CCGCTGCCTG TCTCCTGCAG CTTAAAGGTG TGAAGAGAGC GTGCTGTGAT 
TTTCTTAATA GCCAGTTAGA TCCGTCAAAC TGTCTGGGAA TCAGAGACTT TGCCGAAACG 
CACAACTGTC TGGACTTGAT GCAGGCTGCC GAGCTGTTCT CACAGAAACA CTTTGCTGAG 
GTGGTACAAC AGGAAGAGTT CATGCTGCTC AGTCAGAGTG AAGTGGAAAA ACTCATCAAG 
TGTGATGTGA TCCAGGTTGA TTCAGAGGAG CCAGTATTTG AGGCTGTTTT GAACTGGGTG 
AAGCACAACA GAAAGGAGAG AGAGCCGTAC CTGCCTGATC TGCTGGAATA CGTGCGCATG 
CCCCTGCTCA CCCCTCGATA CATCACTGAT GTCATTGACG CAGAGCCGTT GATACGCTGT 
AGTCTGCCTT GCCGAGATTT GGTGGATGAA GCAAAGAAGT TTCATCTTCG TCCAGAGTTA 
CGCAGCGAAA TGCAGAGTCC TCGAACCCAA GCAAGACTCG GTGCAAAAGA GGTTCTGTTG 
GTTATCGGAG GATTTGGCAG CCAGCAGTCT CCTATTGATA TTGTCGAGAA GTACGATCCC 
AAAACCCGAG AGTGGAGCTT TCTTCCAAAC ATTGCTAGAA AGCGGCGCTA CGTTGCCACT 
GTTGCCTTAA ATGATCGTGT TTATGTTATT GGCGGGTATG ATGGCCGATC GCGGTTGAGT 
TCAGTTGAGT GTTTGGACTA CACAGCTGAT GAAGATGGAG TCTGGTACTC TGTAGCCACA 
ATGAATGTAC GCCGAGGCCT TGCAGGAGCT ACAACACTCG GAGATATGAT CTATGTTGCG 
GGTGGATTCG ATGGAAGTCG ACGTCACACT AGCATGGAGC GATACGACCC CAATATTGAT 
CAGTGGAGCA TGTTGGGAGA TATGCAGACT GCCAGGGAGG GAGCGGGACT TGTTGTGGCC 
AGTGGACTCA TTTACTGTTT AGGTGGTTAT GATGGCCTGA ACATCCTAAA TTCTGTAGAG 
CGGTATGACC CTCACACAGG GCACTGGACC AGCGTCACTC CCATGGCCAA CAAACGCTCA 
GGGGCTGGTG TGGCTTTGCT GAATGATCAC ATATATGTTG TTGGAGGGTT TGACGGCACA 
GCGCATCTGT CTTCAGTAGA GGTCTACAAC ATCCGCACAG ACTACTGGAC CACAGTGGCG 
AATATGACCA CACCACGCTG CTACGTAGGG GCCACCGTCT TAAGGGGCCG ACTTTATGCC 
ATTGCAGGAT ATGATGGGAA CTCACTGCTC AGCAGCATTG AATGTTATGA CCCTGTGATT 
GACAGCTGGG AAGTGGTGAC GTCAATGGCA ACACAACGCT GTGACGCAGG CGTTTGTGTG 
CTCAGAGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007329.1
CDS	203..1897
Translation	MAPKDIMTNSHAKSILNAMNALRKSNTLCDITLRVEGTDFPAHRIVLAACSDYFCAMFTSELAEKGKSFVDIQGLTASTMEILLDFVYTETVLVTVENVQELLPAACLLQLKGVKRACCDFLNSQLDPSNCLGIRDFAETHNCLDLMQAAELFSQKHFAEVVQQEEFMLLSQSEVEKLIKCDVIQVDSEEPVFEAVLNWVKHNRKEREPYLPDLLEYVRMPLLTPRYITDVIDAEPLIRCSLPCRDLVDEAKKFHLRPELRSEMQSPRTQARLGAKEVLLVIGGFGSQQSPIDIVEKYDPKTREWSFLPNIARKRRYVATVALNDRVYVIGGYDGRSRLSSVECLDYTADEDGVWYSVATMNVRRGLAGATTLGDMIYVAGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGLIYCLGGYDGLNILNSVERYDPHTGHWTSVTPMANKRSGAGVALLNDHIYVVGGFDGTAHLSSVEVYNIRTDYWTTVANMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPVIDSWEVVTSMATQRCDAGVCVLREK

Target ORF information:

RefSeq Version	NM_001007328.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kelch-like family member 12 (klhl12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007328.1

ORF Insert Sequence:

ATGGCGCCCA AGGACATCAT GACCAATTCT CATGCCAAAT CCATCTTGAA TGCCATGAAC 
GCCCTGAGAA AGAGCAACAC GCTGTGTGAC ATCACTCTGA GGGTGGAGGG CACAGATTTT 
CCTGCCCACC GCATTGTGTT GGCGGCGTGC AGTGATTATT TCTGCGCCAT GTTCACCAGT 
GAGCTTGCTG AAAAAGGGAA GTCCTTTGTG GACATTCAGG GCCTCACAGC GTCCACTATG 
GAGATCCTGC TGGATTTTGT TTACACAGAG ACAGTGCTGG TCACGGTAGA AAACGTCCAG 
GAGCTGCTGC CCGCTGCCTG TCTCCTGCAG CTTAAAGGTG TGAAGAGAGC GTGCTGTGAT 
TTTCTTAATA GCCAGTTAGA TCCGTCAAAC TGTCTGGGAA TCAGAGACTT TGCCGAAACG 
CACAACTGTC TGGACTTGAT GCAGGCTGCC GAGCTGTTCT CACAGAAACA CTTTGCTGAG 
GTGGTACAAC AGGAAGAGTT CATGCTGCTC AGTCAGAGTG AAGTGGAAAA ACTCATCAAG 
TGTGATGTGA TCCAGGTTGA TTCAGAGGAG CCAGTATTTG AGGCTGTTTT GAACTGGGTG 
AAGCACAACA GAAAGGAGAG AGAGCCGTAC CTGCCTGATC TGCTGGAATA CGTGCGCATG 
CCCCTGCTCA CCCCTCGATA CATCACTGAT GTCATTGACG CAGAGCCGTT GATACGCTGT 
AGTCTGCCTT GCCGAGATTT GGTGGATGAA GCAAAGAAGT TTCATCTTCG TCCAGAGTTA 
CGCAGCGAAA TGCAGAGTCC TCGAACCCAA GCAAGACTCG GTGCAAAAGA GGTTCTGTTG 
GTTATCGGAG GATTTGGCAG CCAGCAGTCT CCTATTGATA TTGTCGAGAA GTACGATCCC 
AAAACCCGAG AGTGGAGCTT TCTTCCAAAC ATTGCTAGAA AGCGGCGCTA CGTTGCCACT 
GTTGCCTTAA ATGATCGTGT TTATGTTATT GGCGGGTATG ATGGCCGATC GCGGTTGAGT 
TCAGTTGAGT GTTTGGACTA CACAGCTGAT GAAGATGGAG TCTGGTACTC TGTAGCCACA 
ATGAATGTAC GCCGAGGCCT TGCAGGAGCT ACAACACTCG GAGATATGAT CTATGTTGCG 
GGTGGATTCG ATGGAAGTCG ACGTCACACT AGCATGGAGC GATACGACCC CAATATTGAT 
CAGTGGAGCA TGTTGGGAGA TATGCAGACT GCCAGGGAGG GAGCGGGACT TGTTGTGGCC 
AGTGGACTCA TTTACTGTTT AGGTGGTTAT GATGGCCTGA ACATCCTAAA TTCTGTAGAG 
CGGTATGACC CTCACACAGG GCACTGGACC AGCGTCACTC CCATGGCCAA CAAACGCTCA 
GGGGCTGGTG TGGCTTTGCT GAATGATCAC ATATATGTTG TTGGAGGGTT TGACGGCACA 
GCGCATCTGT CTTCAGTAGA GGTCTACAAC ATCCGCACAG ACTACTGGAC CACAGTGGCG 
AATATGACCA CACCACGCTG CTACGTAGGG GCCACCGTCT TAAGGGGCCG ACTTTATGCC 
ATTGCAGGAT ATGATGGGAA CTCACTGCTC AGCAGCATTG AATGTTATGA CCCTGTGATT 
GACAGCTGGG AAGTGGTGAC GTCAATGGCA ACACAACGCT GTGACGCAGG CGTTTGTGTG 
CTCAGAGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Saltatory evolution of the ectodermal neural cortex gene family at the vertebrate origin.
Genome biology and evolution5(8)1485-502(2013)
Feiner N,Murakami Y,Breithut L,Mazan S,Meyer A,Kuraku S

Adiponectin and adiponectin receptor genes are coexpressed during zebrafish embryogenesis and regulated by food deprivation.
Developmental dynamics : an official publication of the American Association of Anatomists237(6)1682-90(2008 Jun)
Nishio S,Gibert Y,Bernard L,Brunet F,Triqueneaux G,Laudet V

The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
Nature cell biology8(4)348-57(2006 Apr)
Angers S,Thorpe CJ,Biechele TL,Goldenberg SJ,Zheng N,MacCoss MJ,Moon RT