magi1b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009305951.2	XP_009304226.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_009305953.3	XP_009304228.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
XM_005155835.4	XP_005155892.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
XM_005155830.3	XP_005155887.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_009305953.2	XP_009304228.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_005155834.3	XP_005155891.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
NM_001007063.2	NP_001007064.2	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
XM_021479602.1	XP_021335277.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_009305956.3	XP_009304231.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
XM_005155829.3	XP_005155886.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_009305956.2	XP_009304231.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
XM_009305951.3	XP_009304226.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_005155834.4	XP_005155891.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
XM_005155830.4	XP_005155887.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_005155831.3	XP_005155888.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_009305954.2	XP_009304229.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
XM_005155835.3	XP_005155892.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
XM_005155829.4	XP_005155886.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_005155831.4	XP_005155888.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa16987	XM_005155834.4 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa16988	XM_005155835.4 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa53337	NM_001007063.2 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa41345	XM_009305956.3 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa147872	XM_021479602.1 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa16982	XM_005155829.4 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16983	XM_005155830.4 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16984	XM_005155831.4 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41341	XM_009305951.3 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41342	XM_009305953.3 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41343	XM_009305954.2 Latest version!	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa41342	XM_009305953.2	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41345	XM_009305956.2	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41341	XM_009305951.2	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16987	XM_005155834.3	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa16983	XM_005155830.3	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16988	XM_005155835.3	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa16982	XM_005155829.3	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16984	XM_005155831.3	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

magi1b ( XM_005155834.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155891.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa16987
Clone ID Related Accession (Same CDS sequence)	XM_005155834.3 , XM_005155834.4
Accession Version	XM_005155834.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG TAAGAGGGGA ACTGACCGTT GCCTATAATT TAATGTTCAG TATCTTGGAC 
TCCTTCTCCA TGGGGGTGGC ACCTGCGAAG AAGAGCCCCA AACTGCAACA GTTGGAGCGC 
AAGGACAGTC AGGGCAGCTC CCAGCACAGC GTGTCCAGTC AGCGCAGCAT CCACACAGAC 
TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC AACGAGTCGG CCGCCCCACC ACCCCCGTCC 
CAGCCCTTGC CCAGCCTGCC CCAGGACCCT CCAGTAGACG GAACCCTCCA AAAGAAGCCA 
GATCCCTTTA AAATCTGGGC CCAGTCCAGG AGCATGTATG AAAGTAGGCT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155891.1
CDS	487..3258
Translation	MVSVRGELTVAYNLMFSILDSFSMGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155834.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155834.3

ORF Insert Sequence:

ATGGTTTCAG TAAGAGGGGA ACTGACCGTT GCCTATAATT TAATGTTCAG TATCTTGGAC 
TCCTTCTCCA TGGGGGTGGC ACCTGCGAAG AAGAGCCCCA AACTGCAACA GTTGGAGCGC 
AAGGACAGTC AGGGCAGCTC CCAGCACAGC GTGTCCAGTC AGCGCAGCAT CCACACAGAC 
TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC AACGAGTCGG CCGCCCCACC ACCCCCGTCC 
CAGCCCTTGC CCAGCCTGCC CCAGGACCCT CCAGTAGACG GAACCCTCCA AAAGAAGCCA 
GATCCCTTTA AAATCTGGGC CCAGTCCAGG AGCATGTATG AAAGTAGGCT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155834.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155891.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa16987
Clone ID Related Accession (Same CDS sequence)	XM_005155834.3 , XM_005155834.4
Accession Version	XM_005155834.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG TAAGAGGGGA ACTGACCGTT GCCTATAATT TAATGTTCAG TATCTTGGAC 
TCCTTCTCCA TGGGGGTGGC ACCTGCGAAG AAGAGCCCCA AACTGCAACA GTTGGAGCGC 
AAGGACAGTC AGGGCAGCTC CCAGCACAGC GTGTCCAGTC AGCGCAGCAT CCACACAGAC 
TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC AACGAGTCGG CCGCCCCACC ACCCCCGTCC 
CAGCCCTTGC CCAGCCTGCC CCAGGACCCT CCAGTAGACG GAACCCTCCA AAAGAAGCCA 
GATCCCTTTA AAATCTGGGC CCAGTCCAGG AGCATGTATG AAAGTAGGCT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155891.1
CDS	233..3004
Translation	MVSVRGELTVAYNLMFSILDSFSMGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155834.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155834.4

ORF Insert Sequence:

ATGGTTTCAG TAAGAGGGGA ACTGACCGTT GCCTATAATT TAATGTTCAG TATCTTGGAC 
TCCTTCTCCA TGGGGGTGGC ACCTGCGAAG AAGAGCCCCA AACTGCAACA GTTGGAGCGC 
AAGGACAGTC AGGGCAGCTC CCAGCACAGC GTGTCCAGTC AGCGCAGCAT CCACACAGAC 
TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC AACGAGTCGG CCGCCCCACC ACCCCCGTCC 
CAGCCCTTGC CCAGCCTGCC CCAGGACCCT CCAGTAGACG GAACCCTCCA AAAGAAGCCA 
GATCCCTTTA AAATCTGGGC CCAGTCCAGG AGCATGTATG AAAGTAGGCT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155835.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155892.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa16988
Clone ID Related Accession (Same CDS sequence)	XM_005155835.4 , XM_005155835.3
Accession Version	XM_005155835.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC CTTCGGCAGC AACGTCGCGA GGGGCAGGGA CGCTGGAAGA TGGTTTCAGG 
GTGGCACCTG CGAAGAAGAG CCCCAAACTG CAACAGTTGG AGCGCAAGGA CAGTCAGGGC 
AGCTCCCAGC ACAGCGTGTC CAGTCAGCGC AGCATCCACA CAGACTCTCC TCTCCACGCC 
TCTCAGGCCC TGCTCAACGA GTCGGCCGCC CCACCACCCC CGTCCCAGCC CTTGCCCAGC 
CTGCCCCAGG ACCCTCCAGT AGACGGAACC CTCCAAAAGA AGCCAGATCC CTTTAAAATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTGCCTG ATTACCAGGA GCAGGATATC 
TTCCTGTGGA GGAAAGATAC CGGCTTTGGA TTCAGGATCC TGGGAGGAAA TGAACCTGGT 
GAGCCGATTT ATATTGGCCA CATTGTGAAA TATGGAGCTG CCGATGAAGA TGGGCGTCTG 
CGATCAGGAG ATGAGCTAAT TTGCGTGGAT GGAACGGCCG TTGTTGGAAA ATCTCATCAG 
CTCGTCGTTC AGCTCATGCA ACAAGCTGCA AAACAGGGTC ACGTCAACCT GACCGTGAGG 
CGCAAGACTG GATACGGACT GGCAAAGGTG GACGGTGAAA TGCCTCCTTC TCCTGCCTCC 
TCTCACCACA GCAGCACACA GGCCCCATCT CTGACCGAGG AGAAGCGCAC GCCACAGGGC 
AGCCAGAACT CCCTAAACAC GGTCAGCTCT GGGTCAGGCT CCACCAGCGG TATCGGTAGT 
GGAGGTGGCG GCGGCAGCAA CAACACAGTC ATTCCCAATG CAATCCTGCC CTATGATGTG 
GAGATCCAGC GCGGAGAGAA TGAGGGATTT GGATTTGTCA TTGTGTCGTC GGTGTCGAGG 
CCTGAGGCGG GAACAACTTT CGCTGGAAAC GCATGTGTGG CAATGCCTCA TAAAATAGGT 
CGCATCATCG AGGGAAGCCC AGCCGACCGC TGTGGAAAAC TGAAAGTCGG AGACCGGATC 
CTGGCTGTGA ACGGCTGCTC CATCACCAAC AAGTCCCACT CGGATATCGT CAACCTGATC 
AAAGAGGCTG GAAACACAGT GACGCTGCGG ATCATTCCAG GAGACGAGTC CTCCAATGCA 
TCACTGCTGA CCAATGCTGA GAAGATTGCA ACCATCACTA CCACACACAC ACCTCAACAG 
CAAGCTGCAC CTGAGCCAAG AAACAACACC AAACCAAAAC AGGAGTCTTT TGAGTTCAAA 
GCCCCGCAAG CGCCGCCTCC TCCAGCTCCA ACACAATCTT CAAATCAGGA TGCTGAGTTT 
TACTCTGTGG ATCTGGAGAG GGACAATAAA GGCTTTGGTT TCAGCCTTAG AGGGGGTCGA 
GAGTACAACA TGGACCTGTA TGTGCTGAGA CTCGCTGAGG ACGGAGCGGC TGTCAGGAAT 
GGGAAGATGA GGGTTGGAGA TGAGATCCTG GAGATCAATG GAGAGAGCAC CAAGGGCATG 
AAGCACGCCC GCGCCATTGA ACTGATCAAA AACGGTGGCC GAAAAGTCCA CCTGGTCCTA 
AGGAGGGGGG ACGGCTCGGT GCCAGAATAT GACGGCATCA ACGACGGCAA CGGCCTCGGC 
GCCGGTGCAC AAACTGTTTC GGAAGTGAAC TCAGCTCCTC CCGAAAGTCG GCCCCACCCA 
CCATCGGAGT CCAGCTATCC ACCAGACCTT CACAAATCAT CCCGAAATGA CAACAAAAGG 
ACACGGGAGT CAGGCCAACG GGAGCATGGT CACCGGTCAA ACCACCGCCA CAACAACCAC 
AGACACCGCT CCCCTGAAAA GAAGAGCAGG AGCAAAGGGC ACGGTAAGGA CAGTGGAACT 
TCAAAGTCTC ACAGGAAGAA GGGGGACAAG AAGAAATCTA GTACGGATGA TTCGCATCAT 
CATCACCACC ACCACAGCAA CAGCCGCAAC CAGTACCACC ATCACCGCCA AAGGCACCGC 
TCCCCAGAGA GGCGCAGAAC TCGAAGTGCC GGGGACACCT TAGACAGTAG ACACACCCAG 
AGAAACGGAC GCTCACCAGA CAGGCGGCAC CATCGCCACT CTTCCCCACG CAGACACAGC 
TCACCCCATC GCTCACCTCA CCGATCTGGT TATCGCCGAA GCTCTCCTTA TCGCAACAGA 
TCCCCCTATC GCTCCCCTCA ACGCTCCAGA TACCAGTCTC CCTCAAGACG TCCCACGGAT 
AAAGAGTTCG ACAGGGGAAT TGACGTGACC CTAAAGGAAC CTCCTCCTCC GAAGCCCCCA 
TCCCGCTCTG AGAGCATTCC ACTTTTAGAC CGGCTCAGTC TGGAAGACAC TGTAATGAGA 
GAACCCCCCA GCTACCGTCG TGACCTTTCC CCAATGGAGC GCGCCTATCG AGAGTCGACC 
CTTCTCCGAG GGGCTTCGCG TGATGGCACC TTTCCCCGTG TTCGAACGCC AGAGTCAGAT 
TCAGCTTTCC GGAATTACAA CTCATTACTG AGGGGCCGTT CTCCAGTGAG AGTTGGGAAC 
AGACACGATG CATATCGCAG AGATCAGTCT CCGGTTGCAA GCTCATACCG AACAAGGAGT 
CTAGGTCGAG ACCTGTCTCC CATTCGAGGG TTCCGCGATG AGGAAGAAGA GGAGGTTGAG 
TCTCAGCTGA GCGAGTATTA TAGCCCGCTC CGATCTAATT ACAGCAGAGC TAACAACAAC 
GTACCTGAGC CCAGGAGGAA TGCCTACAGA GAAAGCCCCA AAGATCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155892.1
CDS	195..2951
Translation	MQDPSAATSRGAGTLEDGFRVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155835.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155835.4

ORF Insert Sequence:

ATGCAGGATC CTTCGGCAGC AACGTCGCGA GGGGCAGGGA CGCTGGAAGA TGGTTTCAGG 
GTGGCACCTG CGAAGAAGAG CCCCAAACTG CAACAGTTGG AGCGCAAGGA CAGTCAGGGC 
AGCTCCCAGC ACAGCGTGTC CAGTCAGCGC AGCATCCACA CAGACTCTCC TCTCCACGCC 
TCTCAGGCCC TGCTCAACGA GTCGGCCGCC CCACCACCCC CGTCCCAGCC CTTGCCCAGC 
CTGCCCCAGG ACCCTCCAGT AGACGGAACC CTCCAAAAGA AGCCAGATCC CTTTAAAATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTGCCTG ATTACCAGGA GCAGGATATC 
TTCCTGTGGA GGAAAGATAC CGGCTTTGGA TTCAGGATCC TGGGAGGAAA TGAACCTGGT 
GAGCCGATTT ATATTGGCCA CATTGTGAAA TATGGAGCTG CCGATGAAGA TGGGCGTCTG 
CGATCAGGAG ATGAGCTAAT TTGCGTGGAT GGAACGGCCG TTGTTGGAAA ATCTCATCAG 
CTCGTCGTTC AGCTCATGCA ACAAGCTGCA AAACAGGGTC ACGTCAACCT GACCGTGAGG 
CGCAAGACTG GATACGGACT GGCAAAGGTG GACGGTGAAA TGCCTCCTTC TCCTGCCTCC 
TCTCACCACA GCAGCACACA GGCCCCATCT CTGACCGAGG AGAAGCGCAC GCCACAGGGC 
AGCCAGAACT CCCTAAACAC GGTCAGCTCT GGGTCAGGCT CCACCAGCGG TATCGGTAGT 
GGAGGTGGCG GCGGCAGCAA CAACACAGTC ATTCCCAATG CAATCCTGCC CTATGATGTG 
GAGATCCAGC GCGGAGAGAA TGAGGGATTT GGATTTGTCA TTGTGTCGTC GGTGTCGAGG 
CCTGAGGCGG GAACAACTTT CGCTGGAAAC GCATGTGTGG CAATGCCTCA TAAAATAGGT 
CGCATCATCG AGGGAAGCCC AGCCGACCGC TGTGGAAAAC TGAAAGTCGG AGACCGGATC 
CTGGCTGTGA ACGGCTGCTC CATCACCAAC AAGTCCCACT CGGATATCGT CAACCTGATC 
AAAGAGGCTG GAAACACAGT GACGCTGCGG ATCATTCCAG GAGACGAGTC CTCCAATGCA 
TCACTGCTGA CCAATGCTGA GAAGATTGCA ACCATCACTA CCACACACAC ACCTCAACAG 
CAAGCTGCAC CTGAGCCAAG AAACAACACC AAACCAAAAC AGGAGTCTTT TGAGTTCAAA 
GCCCCGCAAG CGCCGCCTCC TCCAGCTCCA ACACAATCTT CAAATCAGGA TGCTGAGTTT 
TACTCTGTGG ATCTGGAGAG GGACAATAAA GGCTTTGGTT TCAGCCTTAG AGGGGGTCGA 
GAGTACAACA TGGACCTGTA TGTGCTGAGA CTCGCTGAGG ACGGAGCGGC TGTCAGGAAT 
GGGAAGATGA GGGTTGGAGA TGAGATCCTG GAGATCAATG GAGAGAGCAC CAAGGGCATG 
AAGCACGCCC GCGCCATTGA ACTGATCAAA AACGGTGGCC GAAAAGTCCA CCTGGTCCTA 
AGGAGGGGGG ACGGCTCGGT GCCAGAATAT GACGGCATCA ACGACGGCAA CGGCCTCGGC 
GCCGGTGCAC AAACTGTTTC GGAAGTGAAC TCAGCTCCTC CCGAAAGTCG GCCCCACCCA 
CCATCGGAGT CCAGCTATCC ACCAGACCTT CACAAATCAT CCCGAAATGA CAACAAAAGG 
ACACGGGAGT CAGGCCAACG GGAGCATGGT CACCGGTCAA ACCACCGCCA CAACAACCAC 
AGACACCGCT CCCCTGAAAA GAAGAGCAGG AGCAAAGGGC ACGGTAAGGA CAGTGGAACT 
TCAAAGTCTC ACAGGAAGAA GGGGGACAAG AAGAAATCTA GTACGGATGA TTCGCATCAT 
CATCACCACC ACCACAGCAA CAGCCGCAAC CAGTACCACC ATCACCGCCA AAGGCACCGC 
TCCCCAGAGA GGCGCAGAAC TCGAAGTGCC GGGGACACCT TAGACAGTAG ACACACCCAG 
AGAAACGGAC GCTCACCAGA CAGGCGGCAC CATCGCCACT CTTCCCCACG CAGACACAGC 
TCACCCCATC GCTCACCTCA CCGATCTGGT TATCGCCGAA GCTCTCCTTA TCGCAACAGA 
TCCCCCTATC GCTCCCCTCA ACGCTCCAGA TACCAGTCTC CCTCAAGACG TCCCACGGAT 
AAAGAGTTCG ACAGGGGAAT TGACGTGACC CTAAAGGAAC CTCCTCCTCC GAAGCCCCCA 
TCCCGCTCTG AGAGCATTCC ACTTTTAGAC CGGCTCAGTC TGGAAGACAC TGTAATGAGA 
GAACCCCCCA GCTACCGTCG TGACCTTTCC CCAATGGAGC GCGCCTATCG AGAGTCGACC 
CTTCTCCGAG GGGCTTCGCG TGATGGCACC TTTCCCCGTG TTCGAACGCC AGAGTCAGAT 
TCAGCTTTCC GGAATTACAA CTCATTACTG AGGGGCCGTT CTCCAGTGAG AGTTGGGAAC 
AGACACGATG CATATCGCAG AGATCAGTCT CCGGTTGCAA GCTCATACCG AACAAGGAGT 
CTAGGTCGAG ACCTGTCTCC CATTCGAGGG TTCCGCGATG AGGAAGAAGA GGAGGTTGAG 
TCTCAGCTGA GCGAGTATTA TAGCCCGCTC CGATCTAATT ACAGCAGAGC TAACAACAAC 
GTACCTGAGC CCAGGAGGAA TGCCTACAGA GAAAGCCCCA AAGATCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155835.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155892.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa16988
Clone ID Related Accession (Same CDS sequence)	XM_005155835.4 , XM_005155835.3
Accession Version	XM_005155835.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC CTTCGGCAGC AACGTCGCGA GGGGCAGGGA CGCTGGAAGA TGGTTTCAGG 
GTGGCACCTG CGAAGAAGAG CCCCAAACTG CAACAGTTGG AGCGCAAGGA CAGTCAGGGC 
AGCTCCCAGC ACAGCGTGTC CAGTCAGCGC AGCATCCACA CAGACTCTCC TCTCCACGCC 
TCTCAGGCCC TGCTCAACGA GTCGGCCGCC CCACCACCCC CGTCCCAGCC CTTGCCCAGC 
CTGCCCCAGG ACCCTCCAGT AGACGGAACC CTCCAAAAGA AGCCAGATCC CTTTAAAATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTGCCTG ATTACCAGGA GCAGGATATC 
TTCCTGTGGA GGAAAGATAC CGGCTTTGGA TTCAGGATCC TGGGAGGAAA TGAACCTGGT 
GAGCCGATTT ATATTGGCCA CATTGTGAAA TATGGAGCTG CCGATGAAGA TGGGCGTCTG 
CGATCAGGAG ATGAGCTAAT TTGCGTGGAT GGAACGGCCG TTGTTGGAAA ATCTCATCAG 
CTCGTCGTTC AGCTCATGCA ACAAGCTGCA AAACAGGGTC ACGTCAACCT GACCGTGAGG 
CGCAAGACTG GATACGGACT GGCAAAGGTG GACGGTGAAA TGCCTCCTTC TCCTGCCTCC 
TCTCACCACA GCAGCACACA GGCCCCATCT CTGACCGAGG AGAAGCGCAC GCCACAGGGC 
AGCCAGAACT CCCTAAACAC GGTCAGCTCT GGGTCAGGCT CCACCAGCGG TATCGGTAGT 
GGAGGTGGCG GCGGCAGCAA CAACACAGTC ATTCCCAATG CAATCCTGCC CTATGATGTG 
GAGATCCAGC GCGGAGAGAA TGAGGGATTT GGATTTGTCA TTGTGTCGTC GGTGTCGAGG 
CCTGAGGCGG GAACAACTTT CGCTGGAAAC GCATGTGTGG CAATGCCTCA TAAAATAGGT 
CGCATCATCG AGGGAAGCCC AGCCGACCGC TGTGGAAAAC TGAAAGTCGG AGACCGGATC 
CTGGCTGTGA ACGGCTGCTC CATCACCAAC AAGTCCCACT CGGATATCGT CAACCTGATC 
AAAGAGGCTG GAAACACAGT GACGCTGCGG ATCATTCCAG GAGACGAGTC CTCCAATGCA 
TCACTGCTGA CCAATGCTGA GAAGATTGCA ACCATCACTA CCACACACAC ACCTCAACAG 
CAAGCTGCAC CTGAGCCAAG AAACAACACC AAACCAAAAC AGGAGTCTTT TGAGTTCAAA 
GCCCCGCAAG CGCCGCCTCC TCCAGCTCCA ACACAATCTT CAAATCAGGA TGCTGAGTTT 
TACTCTGTGG ATCTGGAGAG GGACAATAAA GGCTTTGGTT TCAGCCTTAG AGGGGGTCGA 
GAGTACAACA TGGACCTGTA TGTGCTGAGA CTCGCTGAGG ACGGAGCGGC TGTCAGGAAT 
GGGAAGATGA GGGTTGGAGA TGAGATCCTG GAGATCAATG GAGAGAGCAC CAAGGGCATG 
AAGCACGCCC GCGCCATTGA ACTGATCAAA AACGGTGGCC GAAAAGTCCA CCTGGTCCTA 
AGGAGGGGGG ACGGCTCGGT GCCAGAATAT GACGGCATCA ACGACGGCAA CGGCCTCGGC 
GCCGGTGCAC AAACTGTTTC GGAAGTGAAC TCAGCTCCTC CCGAAAGTCG GCCCCACCCA 
CCATCGGAGT CCAGCTATCC ACCAGACCTT CACAAATCAT CCCGAAATGA CAACAAAAGG 
ACACGGGAGT CAGGCCAACG GGAGCATGGT CACCGGTCAA ACCACCGCCA CAACAACCAC 
AGACACCGCT CCCCTGAAAA GAAGAGCAGG AGCAAAGGGC ACGGTAAGGA CAGTGGAACT 
TCAAAGTCTC ACAGGAAGAA GGGGGACAAG AAGAAATCTA GTACGGATGA TTCGCATCAT 
CATCACCACC ACCACAGCAA CAGCCGCAAC CAGTACCACC ATCACCGCCA AAGGCACCGC 
TCCCCAGAGA GGCGCAGAAC TCGAAGTGCC GGGGACACCT TAGACAGTAG ACACACCCAG 
AGAAACGGAC GCTCACCAGA CAGGCGGCAC CATCGCCACT CTTCCCCACG CAGACACAGC 
TCACCCCATC GCTCACCTCA CCGATCTGGT TATCGCCGAA GCTCTCCTTA TCGCAACAGA 
TCCCCCTATC GCTCCCCTCA ACGCTCCAGA TACCAGTCTC CCTCAAGACG TCCCACGGAT 
AAAGAGTTCG ACAGGGGAAT TGACGTGACC CTAAAGGAAC CTCCTCCTCC GAAGCCCCCA 
TCCCGCTCTG AGAGCATTCC ACTTTTAGAC CGGCTCAGTC TGGAAGACAC TGTAATGAGA 
GAACCCCCCA GCTACCGTCG TGACCTTTCC CCAATGGAGC GCGCCTATCG AGAGTCGACC 
CTTCTCCGAG GGGCTTCGCG TGATGGCACC TTTCCCCGTG TTCGAACGCC AGAGTCAGAT 
TCAGCTTTCC GGAATTACAA CTCATTACTG AGGGGCCGTT CTCCAGTGAG AGTTGGGAAC 
AGACACGATG CATATCGCAG AGATCAGTCT CCGGTTGCAA GCTCATACCG AACAAGGAGT 
CTAGGTCGAG ACCTGTCTCC CATTCGAGGG TTCCGCGATG AGGAAGAAGA GGAGGTTGAG 
TCTCAGCTGA GCGAGTATTA TAGCCCGCTC CGATCTAATT ACAGCAGAGC TAACAACAAC 
GTACCTGAGC CCAGGAGGAA TGCCTACAGA GAAAGCCCCA AAGATCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155892.1
CDS	438..3194
Translation	MQDPSAATSRGAGTLEDGFRVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155835.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155835.3

ORF Insert Sequence:

ATGCAGGATC CTTCGGCAGC AACGTCGCGA GGGGCAGGGA CGCTGGAAGA TGGTTTCAGG 
GTGGCACCTG CGAAGAAGAG CCCCAAACTG CAACAGTTGG AGCGCAAGGA CAGTCAGGGC 
AGCTCCCAGC ACAGCGTGTC CAGTCAGCGC AGCATCCACA CAGACTCTCC TCTCCACGCC 
TCTCAGGCCC TGCTCAACGA GTCGGCCGCC CCACCACCCC CGTCCCAGCC CTTGCCCAGC 
CTGCCCCAGG ACCCTCCAGT AGACGGAACC CTCCAAAAGA AGCCAGATCC CTTTAAAATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTGCCTG ATTACCAGGA GCAGGATATC 
TTCCTGTGGA GGAAAGATAC CGGCTTTGGA TTCAGGATCC TGGGAGGAAA TGAACCTGGT 
GAGCCGATTT ATATTGGCCA CATTGTGAAA TATGGAGCTG CCGATGAAGA TGGGCGTCTG 
CGATCAGGAG ATGAGCTAAT TTGCGTGGAT GGAACGGCCG TTGTTGGAAA ATCTCATCAG 
CTCGTCGTTC AGCTCATGCA ACAAGCTGCA AAACAGGGTC ACGTCAACCT GACCGTGAGG 
CGCAAGACTG GATACGGACT GGCAAAGGTG GACGGTGAAA TGCCTCCTTC TCCTGCCTCC 
TCTCACCACA GCAGCACACA GGCCCCATCT CTGACCGAGG AGAAGCGCAC GCCACAGGGC 
AGCCAGAACT CCCTAAACAC GGTCAGCTCT GGGTCAGGCT CCACCAGCGG TATCGGTAGT 
GGAGGTGGCG GCGGCAGCAA CAACACAGTC ATTCCCAATG CAATCCTGCC CTATGATGTG 
GAGATCCAGC GCGGAGAGAA TGAGGGATTT GGATTTGTCA TTGTGTCGTC GGTGTCGAGG 
CCTGAGGCGG GAACAACTTT CGCTGGAAAC GCATGTGTGG CAATGCCTCA TAAAATAGGT 
CGCATCATCG AGGGAAGCCC AGCCGACCGC TGTGGAAAAC TGAAAGTCGG AGACCGGATC 
CTGGCTGTGA ACGGCTGCTC CATCACCAAC AAGTCCCACT CGGATATCGT CAACCTGATC 
AAAGAGGCTG GAAACACAGT GACGCTGCGG ATCATTCCAG GAGACGAGTC CTCCAATGCA 
TCACTGCTGA CCAATGCTGA GAAGATTGCA ACCATCACTA CCACACACAC ACCTCAACAG 
CAAGCTGCAC CTGAGCCAAG AAACAACACC AAACCAAAAC AGGAGTCTTT TGAGTTCAAA 
GCCCCGCAAG CGCCGCCTCC TCCAGCTCCA ACACAATCTT CAAATCAGGA TGCTGAGTTT 
TACTCTGTGG ATCTGGAGAG GGACAATAAA GGCTTTGGTT TCAGCCTTAG AGGGGGTCGA 
GAGTACAACA TGGACCTGTA TGTGCTGAGA CTCGCTGAGG ACGGAGCGGC TGTCAGGAAT 
GGGAAGATGA GGGTTGGAGA TGAGATCCTG GAGATCAATG GAGAGAGCAC CAAGGGCATG 
AAGCACGCCC GCGCCATTGA ACTGATCAAA AACGGTGGCC GAAAAGTCCA CCTGGTCCTA 
AGGAGGGGGG ACGGCTCGGT GCCAGAATAT GACGGCATCA ACGACGGCAA CGGCCTCGGC 
GCCGGTGCAC AAACTGTTTC GGAAGTGAAC TCAGCTCCTC CCGAAAGTCG GCCCCACCCA 
CCATCGGAGT CCAGCTATCC ACCAGACCTT CACAAATCAT CCCGAAATGA CAACAAAAGG 
ACACGGGAGT CAGGCCAACG GGAGCATGGT CACCGGTCAA ACCACCGCCA CAACAACCAC 
AGACACCGCT CCCCTGAAAA GAAGAGCAGG AGCAAAGGGC ACGGTAAGGA CAGTGGAACT 
TCAAAGTCTC ACAGGAAGAA GGGGGACAAG AAGAAATCTA GTACGGATGA TTCGCATCAT 
CATCACCACC ACCACAGCAA CAGCCGCAAC CAGTACCACC ATCACCGCCA AAGGCACCGC 
TCCCCAGAGA GGCGCAGAAC TCGAAGTGCC GGGGACACCT TAGACAGTAG ACACACCCAG 
AGAAACGGAC GCTCACCAGA CAGGCGGCAC CATCGCCACT CTTCCCCACG CAGACACAGC 
TCACCCCATC GCTCACCTCA CCGATCTGGT TATCGCCGAA GCTCTCCTTA TCGCAACAGA 
TCCCCCTATC GCTCCCCTCA ACGCTCCAGA TACCAGTCTC CCTCAAGACG TCCCACGGAT 
AAAGAGTTCG ACAGGGGAAT TGACGTGACC CTAAAGGAAC CTCCTCCTCC GAAGCCCCCA 
TCCCGCTCTG AGAGCATTCC ACTTTTAGAC CGGCTCAGTC TGGAAGACAC TGTAATGAGA 
GAACCCCCCA GCTACCGTCG TGACCTTTCC CCAATGGAGC GCGCCTATCG AGAGTCGACC 
CTTCTCCGAG GGGCTTCGCG TGATGGCACC TTTCCCCGTG TTCGAACGCC AGAGTCAGAT 
TCAGCTTTCC GGAATTACAA CTCATTACTG AGGGGCCGTT CTCCAGTGAG AGTTGGGAAC 
AGACACGATG CATATCGCAG AGATCAGTCT CCGGTTGCAA GCTCATACCG AACAAGGAGT 
CTAGGTCGAG ACCTGTCTCC CATTCGAGGG TTCCGCGATG AGGAAGAAGA GGAGGTTGAG 
TCTCAGCTGA GCGAGTATTA TAGCCCGCTC CGATCTAATT ACAGCAGAGC TAACAACAAC 
GTACCTGAGC CCAGGAGGAA TGCCTACAGA GAAAGCCCCA AAGATCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( NM_001007063.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007064.2 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), mRNA.

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CloneID	ODa53337
Clone ID Related Accession (Same CDS sequence)	NM_001007063.2
Accession Version	NM_001007063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX294166.6, CR932038.11 and AL935040.6. On May 25, 2016 this sequence version replaced NM_001007063.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY465352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGGTGGGT CAATCTACGA GAACATCCCC 
TTCTCCCCTG TCTTCACCCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007064.2
CDS	507..4250
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYGGSIYENIPFSPVFTP

Target ORF information:

RefSeq Version	NM_001007063.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007063.2

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGGTGGGT CAATCTACGA GAACATCCCC 
TTCTCCCCTG TCTTCACCCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305956.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304231.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.

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CloneID	ODa41345
Clone ID Related Accession (Same CDS sequence)	XM_009305956.3 , XM_009305956.2
Accession Version	XM_009305956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGCGATGGTT 
GCTCCCCAAG TTGCTGCTTG TATGAGATCT GAAAAGACAG GAGAGCCTGC TTTCTACCAA 
AATCAGACCA CGACGGCATC AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304231.1
CDS	524..4372
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYAMVAPQVAACMRSEKTGEPAFYQNQTTTASTTATASAPVHKLFRK

Target ORF information:

RefSeq Version	XM_009305956.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305956.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGCGATGGTT 
GCTCCCCAAG TTGCTGCTTG TATGAGATCT GAAAAGACAG GAGAGCCTGC TTTCTACCAA 
AATCAGACCA CGACGGCATC AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305956.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304231.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.

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CloneID	ODa41345
Clone ID Related Accession (Same CDS sequence)	XM_009305956.3 , XM_009305956.2
Accession Version	XM_009305956.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGCGATGGTT 
GCTCCCCAAG TTGCTGCTTG TATGAGATCT GAAAAGACAG GAGAGCCTGC TTTCTACCAA 
AATCAGACCA CGACGGCATC AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304231.1
CDS	518..4366
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYAMVAPQVAACMRSEKTGEPAFYQNQTTTASTTATASAPVHKLFRK

Target ORF information:

RefSeq Version	XM_009305956.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305956.2

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGCGATGGTT 
GCTCCCCAAG TTGCTGCTTG TATGAGATCT GAAAAGACAG GAGAGCCTGC TTTCTACCAA 
AATCAGACCA CGACGGCATC AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_021479602.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335277.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.

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CloneID	ODa147872
Clone ID Related Accession (Same CDS sequence)	XM_021479602.1
Accession Version	XM_021479602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAGCTGCCA ACAGGCTGGG AGAAGATCGA TGATCCAGTG 
TACGGGGTGT ATTATGTTGA TCATATTAAC AGGAAGACGC AGTATGAGAA CCCAATTCTG 
GAAGCTAAAA GGAAGAAGCA ACTGGAGCAG TCTCAGCCCG CTGAAGGTGA GCGGTTTATC 
CAAGAATGGA TAGACGAGCA TTCGTCAGGA GCACCGCTAG CGAGCTATGC TGCTAACCAC 
CAAGAGACTT ACAGAGACCC CGGCACCGGC CCTCCACTAC CAACTCAACT GGGGGCTAAA 
CGAGGTAAGC CATTTTTCAC AAGGAACCCT GCAGAACTGA AGGGGACATT TATTAACACC 
AAGCTCAAGA AGAGCAGGCG TGGGTTCGGC TTCACTGTAG TAGGAGGAGA TGAACCCGAT 
GAGTTCTTGC AGATTAAGAG TCTTGTGCTC GATGGACCCG CTGCCCTTGA CGGAAAGATG 
GAAACAGGTG ATGTTATTGT AAGTGTGAAC GACACTTGCG TTTTGGGTTA CACACACGCC 
CAAGTTGTGA AGATCTTCCA GTCAATCCCT ATTGGCTCTA TGGTCGACCT TGAACTCTGC 
CGTGGCTACC CTTTGCCCTT TGACCCTGAT GACCCCAACA CCAGCCTGGT GACTTCAGTG 
GCCATTCTGG ACAAGGAGCC AATCATTGTC AATGGAAAAG AAAGCTACGA CTCACCATCC 
AGCCACGGGA GCGAAACTAC AGCTGGAGGC AGTAGCTTAA ATGGCTCCAG TGATCCTCCT 
CGCCCCCATA GCCCTTCAGC AGAGGTGGCT TCCAATGGCT CTCATGGTTA TCCCAGTGAT 
GTGGTGACTT TGGCGTCCTC GATCGCCACC CAGCCCGAGC TTATTACTGT CCACATGGAA 
AAAGGCGACA AGGGATTCGG CTTCACAATA GCAGACAGCC CGTCTGGAGG AGGCCAGCGA 
GTAAAGCAGA TAGTGGATTA CCCACGATGC AGAGGCCTGA GGGAAGGTGA CATCATCATG 
GAGGTGAACA AGAGGAACGT GCAGAACCTG ACGCACAATC AGGTGGTCGA CCTGCTCAGC 
AAGTGTCCTA AAGGCAGTGA AGTGACCATG TTGGTGCAAC GAGGGGTGGC ACCTGCGAAG 
AAGAGCCCCA AACTGCAACA GTTGGAGCGC AAGGACAGTC AGGGCAGCTC CCAGCACAGC 
GTGTCCAGTC AGCGCAGCAT CCACACAGAC TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC 
AACGAGTCGG CCGCCCCACC ACCCCCGTCC CAGCCCTTGC CCAGCCTGCC CCAGGACCCT 
CCAGTAGACG GAACCCTCCA AAAGAAGCCA GATCCCTTTA AAATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT GCCTGATTAC CAGGAGCAGG ATATCTTCCT GTGGAGGAAA 
GATACCGGCT TTGGATTCAG GATCCTGGGA GGAAATGAAC CTGGTGAGCC GATTTATATT 
GGCCACATTG TGAAATATGG AGCTGCCGAT GAAGATGGGC GTCTGCGATC AGGAGATGAG 
CTAATTTGCG TGGATGGAAC GGCCGTTGTT GGAAAATCTC ATCAGCTCGT CGTTCAGCTC 
ATGCAACAAG CTGCAAAACA GGGTCACGTC AACCTGACCG TGAGGCGCAA GACTGGATAC 
GGACTGGCAA AGGTGGACGG TGAAATGCCT CCTTCTCCTG CCTCCTCTCA CCACAGCAGC 
ACACAGGCCC CATCTCTGAC CGAGGAGAAG CGCACGCCAC AGGGCAGCCA GAACTCCCTA 
AACACGGTCA GCTCTGGGTC AGGCTCCACC AGCGGTATCG GTAGTGGAGG TGGCGGCGGC 
AGCAACAACA CAGTCATTCC CAATGCAATC CTGCCCTATG ATGTGGAGAT CCAGCGCGGA 
GAGAATGAGG GATTTGGATT TGTCATTGTG TCGTCGGTGT CGAGGCCTGA GGCGGGAACA 
ACTTTCGCTG GAAACGCATG TGTGGCAATG CCTCATAAAA TAGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335277.1
CDS	523..5364
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_021479602.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479602.1

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAGCTGCCA ACAGGCTGGG AGAAGATCGA TGATCCAGTG 
TACGGGGTGT ATTATGTTGA TCATATTAAC AGGAAGACGC AGTATGAGAA CCCAATTCTG 
GAAGCTAAAA GGAAGAAGCA ACTGGAGCAG TCTCAGCCCG CTGAAGGTGA GCGGTTTATC 
CAAGAATGGA TAGACGAGCA TTCGTCAGGA GCACCGCTAG CGAGCTATGC TGCTAACCAC 
CAAGAGACTT ACAGAGACCC CGGCACCGGC CCTCCACTAC CAACTCAACT GGGGGCTAAA 
CGAGGTAAGC CATTTTTCAC AAGGAACCCT GCAGAACTGA AGGGGACATT TATTAACACC 
AAGCTCAAGA AGAGCAGGCG TGGGTTCGGC TTCACTGTAG TAGGAGGAGA TGAACCCGAT 
GAGTTCTTGC AGATTAAGAG TCTTGTGCTC GATGGACCCG CTGCCCTTGA CGGAAAGATG 
GAAACAGGTG ATGTTATTGT AAGTGTGAAC GACACTTGCG TTTTGGGTTA CACACACGCC 
CAAGTTGTGA AGATCTTCCA GTCAATCCCT ATTGGCTCTA TGGTCGACCT TGAACTCTGC 
CGTGGCTACC CTTTGCCCTT TGACCCTGAT GACCCCAACA CCAGCCTGGT GACTTCAGTG 
GCCATTCTGG ACAAGGAGCC AATCATTGTC AATGGAAAAG AAAGCTACGA CTCACCATCC 
AGCCACGGGA GCGAAACTAC AGCTGGAGGC AGTAGCTTAA ATGGCTCCAG TGATCCTCCT 
CGCCCCCATA GCCCTTCAGC AGAGGTGGCT TCCAATGGCT CTCATGGTTA TCCCAGTGAT 
GTGGTGACTT TGGCGTCCTC GATCGCCACC CAGCCCGAGC TTATTACTGT CCACATGGAA 
AAAGGCGACA AGGGATTCGG CTTCACAATA GCAGACAGCC CGTCTGGAGG AGGCCAGCGA 
GTAAAGCAGA TAGTGGATTA CCCACGATGC AGAGGCCTGA GGGAAGGTGA CATCATCATG 
GAGGTGAACA AGAGGAACGT GCAGAACCTG ACGCACAATC AGGTGGTCGA CCTGCTCAGC 
AAGTGTCCTA AAGGCAGTGA AGTGACCATG TTGGTGCAAC GAGGGGTGGC ACCTGCGAAG 
AAGAGCCCCA AACTGCAACA GTTGGAGCGC AAGGACAGTC AGGGCAGCTC CCAGCACAGC 
GTGTCCAGTC AGCGCAGCAT CCACACAGAC TCTCCTCTCC ACGCCTCTCA GGCCCTGCTC 
AACGAGTCGG CCGCCCCACC ACCCCCGTCC CAGCCCTTGC CCAGCCTGCC CCAGGACCCT 
CCAGTAGACG GAACCCTCCA AAAGAAGCCA GATCCCTTTA AAATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT GCCTGATTAC CAGGAGCAGG ATATCTTCCT GTGGAGGAAA 
GATACCGGCT TTGGATTCAG GATCCTGGGA GGAAATGAAC CTGGTGAGCC GATTTATATT 
GGCCACATTG TGAAATATGG AGCTGCCGAT GAAGATGGGC GTCTGCGATC AGGAGATGAG 
CTAATTTGCG TGGATGGAAC GGCCGTTGTT GGAAAATCTC ATCAGCTCGT CGTTCAGCTC 
ATGCAACAAG CTGCAAAACA GGGTCACGTC AACCTGACCG TGAGGCGCAA GACTGGATAC 
GGACTGGCAA AGGTGGACGG TGAAATGCCT CCTTCTCCTG CCTCCTCTCA CCACAGCAGC 
ACACAGGCCC CATCTCTGAC CGAGGAGAAG CGCACGCCAC AGGGCAGCCA GAACTCCCTA 
AACACGGTCA GCTCTGGGTC AGGCTCCACC AGCGGTATCG GTAGTGGAGG TGGCGGCGGC 
AGCAACAACA CAGTCATTCC CAATGCAATC CTGCCCTATG ATGTGGAGAT CCAGCGCGGA 
GAGAATGAGG GATTTGGATT TGTCATTGTG TCGTCGGTGT CGAGGCCTGA GGCGGGAACA 
ACTTTCGCTG GAAACGCATG TGTGGCAATG CCTCATAAAA TAGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155829.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155886.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.

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CloneID	ODa16982
Clone ID Related Accession (Same CDS sequence)	XM_005155829.3 , XM_005155829.4
Accession Version	XM_005155829.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGACGGCATC 
AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT CGGAAGTGAA CTCAGCTCCT 
CCCGAAAGTC GGCCCCACCC ACCATCGGAG TCCAGCTATC CACCAGACCT TCACAAATCA 
TCCCGAAATG ACAACAAAAG GACACGGGAG TCAGGCCAAC GGGAGCATGG TCACCGGTCA 
AACCACCGCC ACAACAACCA CAGACACCGC TCCCCTGAAA AGAAGAGCAG GAGCAAAGGG 
CACGGTAAGG ACAGTGGAAC TTCAAAGTCT CACAGGAAGA AGGGGGACAA GAAGAAATCT 
AGTACGGATG ATTCGCATCA TCATCACCAC CACCACAGCA ACAGCCGCAA CCAGTACCAC 
CATCACCGCC AAAGGCACCG CTCCCCAGAG AGGCGCAGAA CTCGAAGTGC CGGGGACACC 
TTAGACAGTA GACACACCCA GAGAAACGGA CGCTCACCAG ACAGGCGGCA CCATCGCCAC 
TCTTCCCCAC GCAGACACAG CTCACCCCAT CGCTCACCTC ACCGATCTGG TTATCGCCGA 
AGCTCTCCTT ATCGCAACAG ATCCCCCTAT CGCTCCCCTC AACGCTCCAG ATACCAGTCT 
CCCTCAAGAC GTCCCACGGA TAAAGAGTTC GACAGGGGAA TTGACGTGAC CCTAAAGGAA 
CCTCCTCCTC CGAAGCCCCC ATCCCGCTCT GAGAGCATTC CACTTTTAGA CCGGCTCAGT 
CTGGAAGACA CTGTAATGAG AGAACCCCCC AGCTACCGTC GTGACCTTTC CCCAATGGAG 
CGCGCCTATC GAGAGTCGAC CCTTCTCCGA GGGGCTTCGC GTGATGGCAC CTTTCCCCGT 
GTTCGAACGC CAGAGTCAGA TTCAGCTTTC CGGAATTACA ACTCATTACT GAGGGGCCGT 
TCTCCAGTGA GAGTTGGGAA CAGACACGAT GCATATCGCA GAGATCAGTC TCCGGTTGCA 
AGCTCATACC GAACAAGGAG TCTAGGTCGA GACCTGTCTC CCATTCGAGG GTTCCGCGAT 
GAGGAAGAAG AGGAGGTTGA GTCTCAGCTG AGCGAGTATT ATAGCCCGCT CCGATCTAAT 
TACAGCAGAG CTAACAACAA CGTACCTGAG CCCAGGAGGA ATGCCTACAG AGAAAGCCCC 
AAAGATCTCA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155886.1
CDS	518..5395
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155829.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155829.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGACGGCATC 
AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT CGGAAGTGAA CTCAGCTCCT 
CCCGAAAGTC GGCCCCACCC ACCATCGGAG TCCAGCTATC CACCAGACCT TCACAAATCA 
TCCCGAAATG ACAACAAAAG GACACGGGAG TCAGGCCAAC GGGAGCATGG TCACCGGTCA 
AACCACCGCC ACAACAACCA CAGACACCGC TCCCCTGAAA AGAAGAGCAG GAGCAAAGGG 
CACGGTAAGG ACAGTGGAAC TTCAAAGTCT CACAGGAAGA AGGGGGACAA GAAGAAATCT 
AGTACGGATG ATTCGCATCA TCATCACCAC CACCACAGCA ACAGCCGCAA CCAGTACCAC 
CATCACCGCC AAAGGCACCG CTCCCCAGAG AGGCGCAGAA CTCGAAGTGC CGGGGACACC 
TTAGACAGTA GACACACCCA GAGAAACGGA CGCTCACCAG ACAGGCGGCA CCATCGCCAC 
TCTTCCCCAC GCAGACACAG CTCACCCCAT CGCTCACCTC ACCGATCTGG TTATCGCCGA 
AGCTCTCCTT ATCGCAACAG ATCCCCCTAT CGCTCCCCTC AACGCTCCAG ATACCAGTCT 
CCCTCAAGAC GTCCCACGGA TAAAGAGTTC GACAGGGGAA TTGACGTGAC CCTAAAGGAA 
CCTCCTCCTC CGAAGCCCCC ATCCCGCTCT GAGAGCATTC CACTTTTAGA CCGGCTCAGT 
CTGGAAGACA CTGTAATGAG AGAACCCCCC AGCTACCGTC GTGACCTTTC CCCAATGGAG 
CGCGCCTATC GAGAGTCGAC CCTTCTCCGA GGGGCTTCGC GTGATGGCAC CTTTCCCCGT 
GTTCGAACGC CAGAGTCAGA TTCAGCTTTC CGGAATTACA ACTCATTACT GAGGGGCCGT 
TCTCCAGTGA GAGTTGGGAA CAGACACGAT GCATATCGCA GAGATCAGTC TCCGGTTGCA 
AGCTCATACC GAACAAGGAG TCTAGGTCGA GACCTGTCTC CCATTCGAGG GTTCCGCGAT 
GAGGAAGAAG AGGAGGTTGA GTCTCAGCTG AGCGAGTATT ATAGCCCGCT CCGATCTAAT 
TACAGCAGAG CTAACAACAA CGTACCTGAG CCCAGGAGGA ATGCCTACAG AGAAAGCCCC 
AAAGATCTCA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155829.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155886.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16982
Clone ID Related Accession (Same CDS sequence)	XM_005155829.3 , XM_005155829.4
Accession Version	XM_005155829.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155829.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGACGGCATC 
AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT CGGAAGTGAA CTCAGCTCCT 
CCCGAAAGTC GGCCCCACCC ACCATCGGAG TCCAGCTATC CACCAGACCT TCACAAATCA 
TCCCGAAATG ACAACAAAAG GACACGGGAG TCAGGCCAAC GGGAGCATGG TCACCGGTCA 
AACCACCGCC ACAACAACCA CAGACACCGC TCCCCTGAAA AGAAGAGCAG GAGCAAAGGG 
CACGGTAAGG ACAGTGGAAC TTCAAAGTCT CACAGGAAGA AGGGGGACAA GAAGAAATCT 
AGTACGGATG ATTCGCATCA TCATCACCAC CACCACAGCA ACAGCCGCAA CCAGTACCAC 
CATCACCGCC AAAGGCACCG CTCCCCAGAG AGGCGCAGAA CTCGAAGTGC CGGGGACACC 
TTAGACAGTA GACACACCCA GAGAAACGGA CGCTCACCAG ACAGGCGGCA CCATCGCCAC 
TCTTCCCCAC GCAGACACAG CTCACCCCAT CGCTCACCTC ACCGATCTGG TTATCGCCGA 
AGCTCTCCTT ATCGCAACAG ATCCCCCTAT CGCTCCCCTC AACGCTCCAG ATACCAGTCT 
CCCTCAAGAC GTCCCACGGA TAAAGAGTTC GACAGGGGAA TTGACGTGAC CCTAAAGGAA 
CCTCCTCCTC CGAAGCCCCC ATCCCGCTCT GAGAGCATTC CACTTTTAGA CCGGCTCAGT 
CTGGAAGACA CTGTAATGAG AGAACCCCCC AGCTACCGTC GTGACCTTTC CCCAATGGAG 
CGCGCCTATC GAGAGTCGAC CCTTCTCCGA GGGGCTTCGC GTGATGGCAC CTTTCCCCGT 
GTTCGAACGC CAGAGTCAGA TTCAGCTTTC CGGAATTACA ACTCATTACT GAGGGGCCGT 
TCTCCAGTGA GAGTTGGGAA CAGACACGAT GCATATCGCA GAGATCAGTC TCCGGTTGCA 
AGCTCATACC GAACAAGGAG TCTAGGTCGA GACCTGTCTC CCATTCGAGG GTTCCGCGAT 
GAGGAAGAAG AGGAGGTTGA GTCTCAGCTG AGCGAGTATT ATAGCCCGCT CCGATCTAAT 
TACAGCAGAG CTAACAACAA CGTACCTGAG CCCAGGAGGA ATGCCTACAG AGAAAGCCCC 
AAAGATCTCA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155886.1
CDS	523..5400
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155829.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155829.4

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGCTGGAAA CGCATGTGTG 
GCAATGCCTC ATAAAATAGG TCGCATCATC GAGGGAAGCC CAGCCGACCG CTGTGGAAAA 
CTGAAAGTCG GAGACCGGAT CCTGGCTGTG AACGGCTGCT CCATCACCAA CAAGTCCCAC 
TCGGATATCG TCAACCTGAT CAAAGAGGCT GGAAACACAG TGACGCTGCG GATCATTCCA 
GGAGACGAGT CCTCCAATGC ATCACTGCTG ACCAATGCTG AGAAGATTGC AACCATCACT 
ACCACACACA CACCTCAACA GCAAGCTGCA CCTGAGCCAA GAAACAACAC CAAACCAAAA 
CAGGAGTCTT TTGAGTTCAA AGCCCCGCAA GCGCCGCCTC CTCCAGCTCC AACACAATCT 
TCAAATCAGG ATGCTGAGTT TTACTCTGTG GATCTGGAGA GGGACAATAA AGGCTTTGGT 
TTCAGCCTTA GAGGGGGTCG AGAGTACAAC ATGGACCTGT ATGTGCTGAG ACTCGCTGAG 
GACGGAGCGG CTGTCAGGAA TGGGAAGATG AGGGTTGGAG ATGAGATCCT GGAGATCAAT 
GGAGAGAGCA CCAAGGGCAT GAAGCACGCC CGCGCCATTG AACTGATCAA AAACGGTGGC 
CGAAAAGTCC ACCTGGTCCT AAGGAGGGGG GACGGCTCGG TGCCAGAATA TGACGGCATC 
AACGACGGCA ACGGCCTCGG CGCCGGTGCA CAAACTGTTT CGGAAGTGAA CTCAGCTCCT 
CCCGAAAGTC GGCCCCACCC ACCATCGGAG TCCAGCTATC CACCAGACCT TCACAAATCA 
TCCCGAAATG ACAACAAAAG GACACGGGAG TCAGGCCAAC GGGAGCATGG TCACCGGTCA 
AACCACCGCC ACAACAACCA CAGACACCGC TCCCCTGAAA AGAAGAGCAG GAGCAAAGGG 
CACGGTAAGG ACAGTGGAAC TTCAAAGTCT CACAGGAAGA AGGGGGACAA GAAGAAATCT 
AGTACGGATG ATTCGCATCA TCATCACCAC CACCACAGCA ACAGCCGCAA CCAGTACCAC 
CATCACCGCC AAAGGCACCG CTCCCCAGAG AGGCGCAGAA CTCGAAGTGC CGGGGACACC 
TTAGACAGTA GACACACCCA GAGAAACGGA CGCTCACCAG ACAGGCGGCA CCATCGCCAC 
TCTTCCCCAC GCAGACACAG CTCACCCCAT CGCTCACCTC ACCGATCTGG TTATCGCCGA 
AGCTCTCCTT ATCGCAACAG ATCCCCCTAT CGCTCCCCTC AACGCTCCAG ATACCAGTCT 
CCCTCAAGAC GTCCCACGGA TAAAGAGTTC GACAGGGGAA TTGACGTGAC CCTAAAGGAA 
CCTCCTCCTC CGAAGCCCCC ATCCCGCTCT GAGAGCATTC CACTTTTAGA CCGGCTCAGT 
CTGGAAGACA CTGTAATGAG AGAACCCCCC AGCTACCGTC GTGACCTTTC CCCAATGGAG 
CGCGCCTATC GAGAGTCGAC CCTTCTCCGA GGGGCTTCGC GTGATGGCAC CTTTCCCCGT 
GTTCGAACGC CAGAGTCAGA TTCAGCTTTC CGGAATTACA ACTCATTACT GAGGGGCCGT 
TCTCCAGTGA GAGTTGGGAA CAGACACGAT GCATATCGCA GAGATCAGTC TCCGGTTGCA 
AGCTCATACC GAACAAGGAG TCTAGGTCGA GACCTGTCTC CCATTCGAGG GTTCCGCGAT 
GAGGAAGAAG AGGAGGTTGA GTCTCAGCTG AGCGAGTATT ATAGCCCGCT CCGATCTAAT 
TACAGCAGAG CTAACAACAA CGTACCTGAG CCCAGGAGGA ATGCCTACAG AGAAAGCCCC 
AAAGATCTCA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155830.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155887.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.

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CloneID	ODa16983
Clone ID Related Accession (Same CDS sequence)	XM_005155830.3 , XM_005155830.4
Accession Version	XM_005155830.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155887.1
CDS	518..5389
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYEMPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155830.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155830.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155830.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155887.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.

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CloneID	ODa16983
Clone ID Related Accession (Same CDS sequence)	XM_005155830.3 , XM_005155830.4
Accession Version	XM_005155830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155830.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155887.1
CDS	523..5394
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYEMPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155830.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155830.4

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAT GCCTGATTAC 
CAGGAGCAGG ATATCTTCCT GTGGAGGAAA GATACCGGCT TTGGATTCAG GATCCTGGGA 
GGAAATGAAC CTGGTGAGCC GATTTATATT GGCCACATTG TGAAATATGG AGCTGCCGAT 
GAAGATGGGC GTCTGCGATC AGGAGATGAG CTAATTTGCG TGGATGGAAC GGCCGTTGTT 
GGAAAATCTC ATCAGCTCGT CGTTCAGCTC ATGCAACAAG CTGCAAAACA GGGTCACGTC 
AACCTGACCG TGAGGCGCAA GACTGGATAC GGACTGGCAA AGGTGGACGG TGAAATGCCT 
CCTTCTCCTG CCTCCTCTCA CCACAGCAGC ACACAGGCCC CATCTCTGAC CGAGGAGAAG 
CGCACGCCAC AGGGCAGCCA GAACTCCCTA AACACGGTCA GCTCTGGGTC AGGCTCCACC 
AGCGGTATCG GTAGTGGAGG TGGCGGCGGC AGCAACAACA CAGTCATTCC CAATGCAATC 
CTGCCCTATG ATGTGGAGAT CCAGCGCGGA GAGAATGAGG GATTTGGATT TGTCATTGTG 
TCGTCGGTGT CGAGGCCTGA GGCGGGAACA ACTTTCGCTG GAAACGCATG TGTGGCAATG 
CCTCATAAAA TAGGTCGCAT CATCGAGGGA AGCCCAGCCG ACCGCTGTGG AAAACTGAAA 
GTCGGAGACC GGATCCTGGC TGTGAACGGC TGCTCCATCA CCAACAAGTC CCACTCGGAT 
ATCGTCAACC TGATCAAAGA GGCTGGAAAC ACAGTGACGC TGCGGATCAT TCCAGGAGAC 
GAGTCCTCCA ATGCATCACT GCTGACCAAT GCTGAGAAGA TTGCAACCAT CACTACCACA 
CACACACCTC AACAGCAAGC TGCACCTGAG CCAAGAAACA ACACCAAACC AAAACAGGAG 
TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG CCTCCTCCAG CTCCAACACA ATCTTCAAAT 
CAGGATGCTG AGTTTTACTC TGTGGATCTG GAGAGGGACA ATAAAGGCTT TGGTTTCAGC 
CTTAGAGGGG GTCGAGAGTA CAACATGGAC CTGTATGTGC TGAGACTCGC TGAGGACGGA 
GCGGCTGTCA GGAATGGGAA GATGAGGGTT GGAGATGAGA TCCTGGAGAT CAATGGAGAG 
AGCACCAAGG GCATGAAGCA CGCCCGCGCC ATTGAACTGA TCAAAAACGG TGGCCGAAAA 
GTCCACCTGG TCCTAAGGAG GGGGGACGGC TCGGTGCCAG AATATGACGG CATCAACGAC 
GGCAACGGCC TCGGCGCCGG TGCACAAACT GTTTCGGAAG TGAACTCAGC TCCTCCCGAA 
AGTCGGCCCC ACCCACCATC GGAGTCCAGC TATCCACCAG ACCTTCACAA ATCATCCCGA 
AATGACAACA AAAGGACACG GGAGTCAGGC CAACGGGAGC ATGGTCACCG GTCAAACCAC 
CGCCACAACA ACCACAGACA CCGCTCCCCT GAAAAGAAGA GCAGGAGCAA AGGGCACGGT 
AAGGACAGTG GAACTTCAAA GTCTCACAGG AAGAAGGGGG ACAAGAAGAA ATCTAGTACG 
GATGATTCGC ATCATCATCA CCACCACCAC AGCAACAGCC GCAACCAGTA CCACCATCAC 
CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC AGAACTCGAA GTGCCGGGGA CACCTTAGAC 
AGTAGACACA CCCAGAGAAA CGGACGCTCA CCAGACAGGC GGCACCATCG CCACTCTTCC 
CCACGCAGAC ACAGCTCACC CCATCGCTCA CCTCACCGAT CTGGTTATCG CCGAAGCTCT 
CCTTATCGCA ACAGATCCCC CTATCGCTCC CCTCAACGCT CCAGATACCA GTCTCCCTCA 
AGACGTCCCA CGGATAAAGA GTTCGACAGG GGAATTGACG TGACCCTAAA GGAACCTCCT 
CCTCCGAAGC CCCCATCCCG CTCTGAGAGC ATTCCACTTT TAGACCGGCT CAGTCTGGAA 
GACACTGTAA TGAGAGAACC CCCCAGCTAC CGTCGTGACC TTTCCCCAAT GGAGCGCGCC 
TATCGAGAGT CGACCCTTCT CCGAGGGGCT TCGCGTGATG GCACCTTTCC CCGTGTTCGA 
ACGCCAGAGT CAGATTCAGC TTTCCGGAAT TACAACTCAT TACTGAGGGG CCGTTCTCCA 
GTGAGAGTTG GGAACAGACA CGATGCATAT CGCAGAGATC AGTCTCCGGT TGCAAGCTCA 
TACCGAACAA GGAGTCTAGG TCGAGACCTG TCTCCCATTC GAGGGTTCCG CGATGAGGAA 
GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG TATTATAGCC CGCTCCGATC TAATTACAGC 
AGAGCTAACA ACAACGTACC TGAGCCCAGG AGGAATGCCT ACAGAGAAAG CCCCAAAGAT 
CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155831.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155888.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.

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CloneID	ODa16984
Clone ID Related Accession (Same CDS sequence)	XM_005155831.3 , XM_005155831.4
Accession Version	XM_005155831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155888.1
CDS	518..5359
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155831.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155831.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_005155831.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155888.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.

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CloneID	ODa16984
Clone ID Related Accession (Same CDS sequence)	XM_005155831.3 , XM_005155831.4
Accession Version	XM_005155831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155888.1
CDS	524..5365
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_005155831.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155831.4

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGTGAGCGG TTTATCCAAG AATGGATAGA CGAGCATTCG 
TCAGGAGCAC CGCTAGCGAG CTATGCTGCT AACCACCAAG AGACTTACAG AGACCCCGGC 
ACCGGCCCTC CACTACCAAC TCAACTGGGG GCTAAACGAG GTAAGCCATT TTTCACAAGG 
AACCCTGCAG AACTGAAGGG GACATTTATT AACACCAAGC TCAAGAAGAG CAGGCGTGGG 
TTCGGCTTCA CTGTAGTAGG AGGAGATGAA CCCGATGAGT TCTTGCAGAT TAAGAGTCTT 
GTGCTCGATG GACCCGCTGC CCTTGACGGA AAGATGGAAA CAGGTGATGT TATTGTAAGT 
GTGAACGACA CTTGCGTTTT GGGTTACACA CACGCCCAAG TTGTGAAGAT CTTCCAGTCA 
ATCCCTATTG GCTCTATGGT CGACCTTGAA CTCTGCCGTG GCTACCCTTT GCCCTTTGAC 
CCTGATGACC CCAACACCAG CCTGGTGACT TCAGTGGCCA TTCTGGACAA GGAGCCAATC 
ATTGTCAATG GAAAAGAAAG CTACGACTCA CCATCCAGCC ACGGGAGCGA AACTACAGCT 
GGAGGCAGTA GCTTAAATGG CTCCAGTGAT CCTCCTCGCC CCCATAGCCC TTCAGCAGAG 
GTGGCTTCCA ATGGCTCTCA TGGTTATCCC AGTGATGTGG TGACTTTGGC GTCCTCGATC 
GCCACCCAGC CCGAGCTTAT TACTGTCCAC ATGGAAAAAG GCGACAAGGG ATTCGGCTTC 
ACAATAGCAG ACAGCCCGTC TGGAGGAGGC CAGCGAGTAA AGCAGATAGT GGATTACCCA 
CGATGCAGAG GCCTGAGGGA AGGTGACATC ATCATGGAGG TGAACAAGAG GAACGTGCAG 
AACCTGACGC ACAATCAGGT GGTCGACCTG CTCAGCAAGT GTCCTAAAGG CAGTGAAGTG 
ACCATGTTGG TGCAACGAGG GGTGGCACCT GCGAAGAAGA GCCCCAAACT GCAACAGTTG 
GAGCGCAAGG ACAGTCAGGG CAGCTCCCAG CACAGCGTGT CCAGTCAGCG CAGCATCCAC 
ACAGACTCTC CTCTCCACGC CTCTCAGGCC CTGCTCAACG AGTCGGCCGC CCCACCACCC 
CCGTCCCAGC CCTTGCCCAG CCTGCCCCAG GACCCTCCAG TAGACGGAAC CCTCCAAAAG 
AAGCCAGATC CCTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTGCCT 
GATTACCAGG AGCAGGATAT CTTCCTGTGG AGGAAAGATA CCGGCTTTGG ATTCAGGATC 
CTGGGAGGAA ATGAACCTGG TGAGCCGATT TATATTGGCC ACATTGTGAA ATATGGAGCT 
GCCGATGAAG ATGGGCGTCT GCGATCAGGA GATGAGCTAA TTTGCGTGGA TGGAACGGCC 
GTTGTTGGAA AATCTCATCA GCTCGTCGTT CAGCTCATGC AACAAGCTGC AAAACAGGGT 
CACGTCAACC TGACCGTGAG GCGCAAGACT GGATACGGAC TGGCAAAGGT GGACGGTGAA 
ATGCCTCCTT CTCCTGCCTC CTCTCACCAC AGCAGCACAC AGGCCCCATC TCTGACCGAG 
GAGAAGCGCA CGCCACAGGG CAGCCAGAAC TCCCTAAACA CGGTCAGCTC TGGGTCAGGC 
TCCACCAGCG GTATCGGTAG TGGAGGTGGC GGCGGCAGCA ACAACACAGT CATTCCCAAT 
GCAATCCTGC CCTATGATGT GGAGATCCAG CGCGGAGAGA ATGAGGGATT TGGATTTGTC 
ATTGTGTCGT CGGTGTCGAG GCCTGAGGCG GGAACAACTT TCGGTCGCAT CATCGAGGGA 
AGCCCAGCCG ACCGCTGTGG AAAACTGAAA GTCGGAGACC GGATCCTGGC TGTGAACGGC 
TGCTCCATCA CCAACAAGTC CCACTCGGAT ATCGTCAACC TGATCAAAGA GGCTGGAAAC 
ACAGTGACGC TGCGGATCAT TCCAGGAGAC GAGTCCTCCA ATGCATCACT GCTGACCAAT 
GCTGAGAAGA TTGCAACCAT CACTACCACA CACACACCTC AACAGCAAGC TGCACCTGAG 
CCAAGAAACA ACACCAAACC AAAACAGGAG TCTTTTGAGT TCAAAGCCCC GCAAGCGCCG 
CCTCCTCCAG CTCCAACACA ATCTTCAAAT CAGGATGCTG AGTTTTACTC TGTGGATCTG 
GAGAGGGACA ATAAAGGCTT TGGTTTCAGC CTTAGAGGGG GTCGAGAGTA CAACATGGAC 
CTGTATGTGC TGAGACTCGC TGAGGACGGA GCGGCTGTCA GGAATGGGAA GATGAGGGTT 
GGAGATGAGA TCCTGGAGAT CAATGGAGAG AGCACCAAGG GCATGAAGCA CGCCCGCGCC 
ATTGAACTGA TCAAAAACGG TGGCCGAAAA GTCCACCTGG TCCTAAGGAG GGGGGACGGC 
TCGGTGCCAG AATATGACGG CATCAACGAC GGCAACGGCC TCGGCGCCGG TGCACAAACT 
GTTTCGGAAG TGAACTCAGC TCCTCCCGAA AGTCGGCCCC ACCCACCATC GGAGTCCAGC 
TATCCACCAG ACCTTCACAA ATCATCCCGA AATGACAACA AAAGGACACG GGAGTCAGGC 
CAACGGGAGC ATGGTCACCG GTCAAACCAC CGCCACAACA ACCACAGACA CCGCTCCCCT 
GAAAAGAAGA GCAGGAGCAA AGGGCACGGT AAGGACAGTG GAACTTCAAA GTCTCACAGG 
AAGAAGGGGG ACAAGAAGAA ATCTAGTACG GATGATTCGC ATCATCATCA CCACCACCAC 
AGCAACAGCC GCAACCAGTA CCACCATCAC CGCCAAAGGC ACCGCTCCCC AGAGAGGCGC 
AGAACTCGAA GTGCCGGGGA CACCTTAGAC AGTAGACACA CCCAGAGAAA CGGACGCTCA 
CCAGACAGGC GGCACCATCG CCACTCTTCC CCACGCAGAC ACAGCTCACC CCATCGCTCA 
CCTCACCGAT CTGGTTATCG CCGAAGCTCT CCTTATCGCA ACAGATCCCC CTATCGCTCC 
CCTCAACGCT CCAGATACCA GTCTCCCTCA AGACGTCCCA CGGATAAAGA GTTCGACAGG 
GGAATTGACG TGACCCTAAA GGAACCTCCT CCTCCGAAGC CCCCATCCCG CTCTGAGAGC 
ATTCCACTTT TAGACCGGCT CAGTCTGGAA GACACTGTAA TGAGAGAACC CCCCAGCTAC 
CGTCGTGACC TTTCCCCAAT GGAGCGCGCC TATCGAGAGT CGACCCTTCT CCGAGGGGCT 
TCGCGTGATG GCACCTTTCC CCGTGTTCGA ACGCCAGAGT CAGATTCAGC TTTCCGGAAT 
TACAACTCAT TACTGAGGGG CCGTTCTCCA GTGAGAGTTG GGAACAGACA CGATGCATAT 
CGCAGAGATC AGTCTCCGGT TGCAAGCTCA TACCGAACAA GGAGTCTAGG TCGAGACCTG 
TCTCCCATTC GAGGGTTCCG CGATGAGGAA GAAGAGGAGG TTGAGTCTCA GCTGAGCGAG 
TATTATAGCC CGCTCCGATC TAATTACAGC AGAGCTAACA ACAACGTACC TGAGCCCAGG 
AGGAATGCCT ACAGAGAAAG CCCCAAAGAT CTCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305951.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304226.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa41341
Clone ID Related Accession (Same CDS sequence)	XM_009305951.2 , XM_009305951.3
Accession Version	XM_009305951.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGACGGCA TCAACGACGG CAACGGCCTC 
GGCGCCGGTG CACAAACTGT TTCGGAAGTG AACTCAGCTC CTCCCGAAAG TCGGCCCCAC 
CCACCATCGG AGTCCAGCTA TCCACCAGAC CTTCACAAAT CATCCCGAAA TGACAACAAA 
AGGACACGGG AGTCAGGCCA ACGGGAGCAT GGTCACCGGT CAAACCACCG CCACAACAAC 
CACAGACACC GCTCCCCTGA AAAGAAGAGC AGGAGCAAAG GGCACGGTAA GGACAGTGGA 
ACTTCAAAGT CTCACAGGAA GAAGGGGGAC AAGAAGAAAT CTAGTACGGA TGATTCGCAT 
CATCATCACC ACCACCACAG CAACAGCCGC AACCAGTACC ACCATCACCG CCAAAGGCAC 
CGCTCCCCAG AGAGGCGCAG AACTCGAAGT GCCGGGGACA CCTTAGACAG TAGACACACC 
CAGAGAAACG GACGCTCACC AGACAGGCGG CACCATCGCC ACTCTTCCCC ACGCAGACAC 
AGCTCACCCC ATCGCTCACC TCACCGATCT GGTTATCGCC GAAGCTCTCC TTATCGCAAC 
AGATCCCCCT ATCGCTCCCC TCAACGCTCC AGATACCAGT CTCCCTCAAG ACGTCCCACG 
GATAAAGAGT TCGACAGGGG AATTGACGTG ACCCTAAAGG AACCTCCTCC TCCGAAGCCC 
CCATCCCGCT CTGAGAGCAT TCCACTTTTA GACCGGCTCA GTCTGGAAGA CACTGTAATG 
AGAGAACCCC CCAGCTACCG TCGTGACCTT TCCCCAATGG AGCGCGCCTA TCGAGAGTCG 
ACCCTTCTCC GAGGGGCTTC GCGTGATGGC ACCTTTCCCC GTGTTCGAAC GCCAGAGTCA 
GATTCAGCTT TCCGGAATTA CAACTCATTA CTGAGGGGCC GTTCTCCAGT GAGAGTTGGG 
AACAGACACG ATGCATATCG CAGAGATCAG TCTCCGGTTG CAAGCTCATA CCGAACAAGG 
AGTCTAGGTC GAGACCTGTC TCCCATTCGA GGGTTCCGCG ATGAGGAAGA AGAGGAGGTT 
GAGTCTCAGC TGAGCGAGTA TTATAGCCCG CTCCGATCTA ATTACAGCAG AGCTAACAAC 
AACGTACCTG AGCCCAGGAG GAATGCCTAC AGAGAAAGCC CCAAAGATCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304226.1
CDS	518..5377
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_009305951.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305951.2

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGACGGCA TCAACGACGG CAACGGCCTC 
GGCGCCGGTG CACAAACTGT TTCGGAAGTG AACTCAGCTC CTCCCGAAAG TCGGCCCCAC 
CCACCATCGG AGTCCAGCTA TCCACCAGAC CTTCACAAAT CATCCCGAAA TGACAACAAA 
AGGACACGGG AGTCAGGCCA ACGGGAGCAT GGTCACCGGT CAAACCACCG CCACAACAAC 
CACAGACACC GCTCCCCTGA AAAGAAGAGC AGGAGCAAAG GGCACGGTAA GGACAGTGGA 
ACTTCAAAGT CTCACAGGAA GAAGGGGGAC AAGAAGAAAT CTAGTACGGA TGATTCGCAT 
CATCATCACC ACCACCACAG CAACAGCCGC AACCAGTACC ACCATCACCG CCAAAGGCAC 
CGCTCCCCAG AGAGGCGCAG AACTCGAAGT GCCGGGGACA CCTTAGACAG TAGACACACC 
CAGAGAAACG GACGCTCACC AGACAGGCGG CACCATCGCC ACTCTTCCCC ACGCAGACAC 
AGCTCACCCC ATCGCTCACC TCACCGATCT GGTTATCGCC GAAGCTCTCC TTATCGCAAC 
AGATCCCCCT ATCGCTCCCC TCAACGCTCC AGATACCAGT CTCCCTCAAG ACGTCCCACG 
GATAAAGAGT TCGACAGGGG AATTGACGTG ACCCTAAAGG AACCTCCTCC TCCGAAGCCC 
CCATCCCGCT CTGAGAGCAT TCCACTTTTA GACCGGCTCA GTCTGGAAGA CACTGTAATG 
AGAGAACCCC CCAGCTACCG TCGTGACCTT TCCCCAATGG AGCGCGCCTA TCGAGAGTCG 
ACCCTTCTCC GAGGGGCTTC GCGTGATGGC ACCTTTCCCC GTGTTCGAAC GCCAGAGTCA 
GATTCAGCTT TCCGGAATTA CAACTCATTA CTGAGGGGCC GTTCTCCAGT GAGAGTTGGG 
AACAGACACG ATGCATATCG CAGAGATCAG TCTCCGGTTG CAAGCTCATA CCGAACAAGG 
AGTCTAGGTC GAGACCTGTC TCCCATTCGA GGGTTCCGCG ATGAGGAAGA AGAGGAGGTT 
GAGTCTCAGC TGAGCGAGTA TTATAGCCCG CTCCGATCTA ATTACAGCAG AGCTAACAAC 
AACGTACCTG AGCCCAGGAG GAATGCCTAC AGAGAAAGCC CCAAAGATCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305951.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304226.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa41341
Clone ID Related Accession (Same CDS sequence)	XM_009305951.2 , XM_009305951.3
Accession Version	XM_009305951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGACGGCA TCAACGACGG CAACGGCCTC 
GGCGCCGGTG CACAAACTGT TTCGGAAGTG AACTCAGCTC CTCCCGAAAG TCGGCCCCAC 
CCACCATCGG AGTCCAGCTA TCCACCAGAC CTTCACAAAT CATCCCGAAA TGACAACAAA 
AGGACACGGG AGTCAGGCCA ACGGGAGCAT GGTCACCGGT CAAACCACCG CCACAACAAC 
CACAGACACC GCTCCCCTGA AAAGAAGAGC AGGAGCAAAG GGCACGGTAA GGACAGTGGA 
ACTTCAAAGT CTCACAGGAA GAAGGGGGAC AAGAAGAAAT CTAGTACGGA TGATTCGCAT 
CATCATCACC ACCACCACAG CAACAGCCGC AACCAGTACC ACCATCACCG CCAAAGGCAC 
CGCTCCCCAG AGAGGCGCAG AACTCGAAGT GCCGGGGACA CCTTAGACAG TAGACACACC 
CAGAGAAACG GACGCTCACC AGACAGGCGG CACCATCGCC ACTCTTCCCC ACGCAGACAC 
AGCTCACCCC ATCGCTCACC TCACCGATCT GGTTATCGCC GAAGCTCTCC TTATCGCAAC 
AGATCCCCCT ATCGCTCCCC TCAACGCTCC AGATACCAGT CTCCCTCAAG ACGTCCCACG 
GATAAAGAGT TCGACAGGGG AATTGACGTG ACCCTAAAGG AACCTCCTCC TCCGAAGCCC 
CCATCCCGCT CTGAGAGCAT TCCACTTTTA GACCGGCTCA GTCTGGAAGA CACTGTAATG 
AGAGAACCCC CCAGCTACCG TCGTGACCTT TCCCCAATGG AGCGCGCCTA TCGAGAGTCG 
ACCCTTCTCC GAGGGGCTTC GCGTGATGGC ACCTTTCCCC GTGTTCGAAC GCCAGAGTCA 
GATTCAGCTT TCCGGAATTA CAACTCATTA CTGAGGGGCC GTTCTCCAGT GAGAGTTGGG 
AACAGACACG ATGCATATCG CAGAGATCAG TCTCCGGTTG CAAGCTCATA CCGAACAAGG 
AGTCTAGGTC GAGACCTGTC TCCCATTCGA GGGTTCCGCG ATGAGGAAGA AGAGGAGGTT 
GAGTCTCAGC TGAGCGAGTA TTATAGCCCG CTCCGATCTA ATTACAGCAG AGCTAACAAC 
AACGTACCTG AGCCCAGGAG GAATGCCTAC AGAGAAAGCC CCAAAGATCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304226.1
CDS	523..5382
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_009305951.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305951.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGAATGGATA GACGAGCATT CGTCAGGAGC ACCGCTAGCG 
AGCTATGCTG CTAACCACCA AGAGACTTAC AGAGACCCCG GCACCGGCCC TCCACTACCA 
ACTCAACTGG GGGCTAAACG AGGTAAGCCA TTTTTCACAA GGAACCCTGC AGAACTGAAG 
GGGACATTTA TTAACACCAA GCTCAAGAAG AGCAGGCGTG GGTTCGGCTT CACTGTAGTA 
GGAGGAGATG AACCCGATGA GTTCTTGCAG ATTAAGAGTC TTGTGCTCGA TGGACCCGCT 
GCCCTTGACG GAAAGATGGA AACAGGTGAT GTTATTGTAA GTGTGAACGA CACTTGCGTT 
TTGGGTTACA CACACGCCCA AGTTGTGAAG ATCTTCCAGT CAATCCCTAT TGGCTCTATG 
GTCGACCTTG AACTCTGCCG TGGCTACCCT TTGCCCTTTG ACCCTGATGA CCCCAACACC 
AGCCTGGTGA CTTCAGTGGC CATTCTGGAC AAGGAGCCAA TCATTGTCAA TGGAAAAGAA 
AGCTACGACT CACCATCCAG CCACGGGAGC GAAACTACAG CTGGAGGCAG TAGCTTAAAT 
GGCTCCAGTG ATCCTCCTCG CCCCCATAGC CCTTCAGCAG AGGTGGCTTC CAATGGCTCT 
CATGGTTATC CCAGTGATGT GGTGACTTTG GCGTCCTCGA TCGCCACCCA GCCCGAGCTT 
ATTACTGTCC ACATGGAAAA AGGCGACAAG GGATTCGGCT TCACAATAGC AGACAGCCCG 
TCTGGAGGAG GCCAGCGAGT AAAGCAGATA GTGGATTACC CACGATGCAG AGGCCTGAGG 
GAAGGTGACA TCATCATGGA GGTGAACAAG AGGAACGTGC AGAACCTGAC GCACAATCAG 
GTGGTCGACC TGCTCAGCAA GTGTCCTAAA GGCAGTGAAG TGACCATGTT GGTGCAACGA 
GGGGTGGCAC CTGCGAAGAA GAGCCCCAAA CTGCAACAGT TGGAGCGCAA GGACAGTCAG 
GGCAGCTCCC AGCACAGCGT GTCCAGTCAG CGCAGCATCC ACACAGACTC TCCTCTCCAC 
GCCTCTCAGG CCCTGCTCAA CGAGTCGGCC GCCCCACCAC CCCCGTCCCA GCCCTTGCCC 
AGCCTGCCCC AGGACCCTCC AGTAGACGGA ACCCTCCAAA AGAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCCAGGAG CATGTATGAA AGTAGGCTGC CTGATTACCA GGAGCAGGAT 
ATCTTCCTGT GGAGGAAAGA TACCGGCTTT GGATTCAGGA TCCTGGGAGG AAATGAACCT 
GGTGAGCCGA TTTATATTGG CCACATTGTG AAATATGGAG CTGCCGATGA AGATGGGCGT 
CTGCGATCAG GAGATGAGCT AATTTGCGTG GATGGAACGG CCGTTGTTGG AAAATCTCAT 
CAGCTCGTCG TTCAGCTCAT GCAACAAGCT GCAAAACAGG GTCACGTCAA CCTGACCGTG 
AGGCGCAAGA CTGGATACGG ACTGGCAAAG GTGGACGGTG AAATGCCTCC TTCTCCTGCC 
TCCTCTCACC ACAGCAGCAC ACAGGCCCCA TCTCTGACCG AGGAGAAGCG CACGCCACAG 
GGCAGCCAGA ACTCCCTAAA CACGGTCAGC TCTGGGTCAG GCTCCACCAG CGGTATCGGT 
AGTGGAGGTG GCGGCGGCAG CAACAACACA GTCATTCCCA ATGCAATCCT GCCCTATGAT 
GTGGAGATCC AGCGCGGAGA GAATGAGGGA TTTGGATTTG TCATTGTGTC GTCGGTGTCG 
AGGCCTGAGG CGGGAACAAC TTTCGCTGGA AACGCATGTG TGGCAATGCC TCATAAAATA 
GGTCGCATCA TCGAGGGAAG CCCAGCCGAC CGCTGTGGAA AACTGAAAGT CGGAGACCGG 
ATCCTGGCTG TGAACGGCTG CTCCATCACC AACAAGTCCC ACTCGGATAT CGTCAACCTG 
ATCAAAGAGG CTGGAAACAC AGTGACGCTG CGGATCATTC CAGGAGACGA GTCCTCCAAT 
GCATCACTGC TGACCAATGC TGAGAAGATT GCAACCATCA CTACCACACA CACACCTCAA 
CAGCAAGCTG CACCTGAGCC AAGAAACAAC ACCAAACCAA AACAGGAGTC TTTTGAGTTC 
AAAGCCCCGC AAGCGCCGCC TCCTCCAGCT CCAACACAAT CTTCAAATCA GGATGCTGAG 
TTTTACTCTG TGGATCTGGA GAGGGACAAT AAAGGCTTTG GTTTCAGCCT TAGAGGGGGT 
CGAGAGTACA ACATGGACCT GTATGTGCTG AGACTCGCTG AGGACGGAGC GGCTGTCAGG 
AATGGGAAGA TGAGGGTTGG AGATGAGATC CTGGAGATCA ATGGAGAGAG CACCAAGGGC 
ATGAAGCACG CCCGCGCCAT TGAACTGATC AAAAACGGTG GCCGAAAAGT CCACCTGGTC 
CTAAGGAGGG GGGACGGCTC GGTGCCAGAA TATGACGGCA TCAACGACGG CAACGGCCTC 
GGCGCCGGTG CACAAACTGT TTCGGAAGTG AACTCAGCTC CTCCCGAAAG TCGGCCCCAC 
CCACCATCGG AGTCCAGCTA TCCACCAGAC CTTCACAAAT CATCCCGAAA TGACAACAAA 
AGGACACGGG AGTCAGGCCA ACGGGAGCAT GGTCACCGGT CAAACCACCG CCACAACAAC 
CACAGACACC GCTCCCCTGA AAAGAAGAGC AGGAGCAAAG GGCACGGTAA GGACAGTGGA 
ACTTCAAAGT CTCACAGGAA GAAGGGGGAC AAGAAGAAAT CTAGTACGGA TGATTCGCAT 
CATCATCACC ACCACCACAG CAACAGCCGC AACCAGTACC ACCATCACCG CCAAAGGCAC 
CGCTCCCCAG AGAGGCGCAG AACTCGAAGT GCCGGGGACA CCTTAGACAG TAGACACACC 
CAGAGAAACG GACGCTCACC AGACAGGCGG CACCATCGCC ACTCTTCCCC ACGCAGACAC 
AGCTCACCCC ATCGCTCACC TCACCGATCT GGTTATCGCC GAAGCTCTCC TTATCGCAAC 
AGATCCCCCT ATCGCTCCCC TCAACGCTCC AGATACCAGT CTCCCTCAAG ACGTCCCACG 
GATAAAGAGT TCGACAGGGG AATTGACGTG ACCCTAAAGG AACCTCCTCC TCCGAAGCCC 
CCATCCCGCT CTGAGAGCAT TCCACTTTTA GACCGGCTCA GTCTGGAAGA CACTGTAATG 
AGAGAACCCC CCAGCTACCG TCGTGACCTT TCCCCAATGG AGCGCGCCTA TCGAGAGTCG 
ACCCTTCTCC GAGGGGCTTC GCGTGATGGC ACCTTTCCCC GTGTTCGAAC GCCAGAGTCA 
GATTCAGCTT TCCGGAATTA CAACTCATTA CTGAGGGGCC GTTCTCCAGT GAGAGTTGGG 
AACAGACACG ATGCATATCG CAGAGATCAG TCTCCGGTTG CAAGCTCATA CCGAACAAGG 
AGTCTAGGTC GAGACCTGTC TCCCATTCGA GGGTTCCGCG ATGAGGAAGA AGAGGAGGTT 
GAGTCTCAGC TGAGCGAGTA TTATAGCCCG CTCCGATCTA ATTACAGCAG AGCTAACAAC 
AACGTACCTG AGCCCAGGAG GAATGCCTAC AGAGAAAGCC CCAAAGATCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305953.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304228.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.

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CloneID	ODa41342
Clone ID Related Accession (Same CDS sequence)	XM_009305953.3 , XM_009305953.2
Accession Version	XM_009305953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGAGGTAAG CCATTTTTCA CAAGGAACCC TGCAGAACTG 
AAGGGGACAT TTATTAACAC CAAGCTCAAG AAGAGCAGGC GTGGGTTCGG CTTCACTGTA 
GTAGGAGGAG ATGAACCCGA TGAGTTCTTG CAGATTAAGA GTCTTGTGCT CGATGGACCC 
GCTGCCCTTG ACGGAAAGAT GGAAACAGGT GATGTTATTG TAAGTGTGAA CGACACTTGC 
GTTTTGGGTT ACACACACGC CCAAGTTGTG AAGATCTTCC AGTCAATCCC TATTGGCTCT 
ATGGTCGACC TTGAACTCTG CCGTGGCTAC CCTTTGCCCT TTGACCCTGA TGACCCCAAC 
ACCAGCCTGG TGACTTCAGT GGCCATTCTG GACAAGGAGC CAATCATTGT CAATGGAAAA 
GAAAGCTACG ACTCACCATC CAGCCACGGG AGCGAAACTA CAGCTGGAGG CAGTAGCTTA 
AATGGCTCCA GTGATCCTCC TCGCCCCCAT AGCCCTTCAG CAGAGGTGGC TTCCAATGGC 
TCTCATGGTT ATCCCAGTGA TGTGGTGACT TTGGCGTCCT CGATCGCCAC CCAGCCCGAG 
CTTATTACTG TCCACATGGA AAAAGGCGAC AAGGGATTCG GCTTCACAAT AGCAGACAGC 
CCGTCTGGAG GAGGCCAGCG AGTAAAGCAG ATAGTGGATT ACCCACGATG CAGAGGCCTG 
AGGGAAGGTG ACATCATCAT GGAGGTGAAC AAGAGGAACG TGCAGAACCT GACGCACAAT 
CAGGTGGTCG ACCTGCTCAG CAAGTGTCCT AAAGGCAGTG AAGTGACCAT GTTGGTGCAA 
CGAGGGGTGG CACCTGCGAA GAAGAGCCCC AAACTGCAAC AGTTGGAGCG CAAGGACAGT 
CAGGGCAGCT CCCAGCACAG CGTGTCCAGT CAGCGCAGCA TCCACACAGA CTCTCCTCTC 
CACGCCTCTC AGGCCCTGCT CAACGAGTCG GCCGCCCCAC CACCCCCGTC CCAGCCCTTG 
CCCAGCCTGC CCCAGGACCC TCCAGTAGAC GGAACCCTCC AAAAGAAGCC AGATCCCTTT 
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAGTAGGC TGCCTGATTA CCAGGAGCAG 
GATATCTTCC TGTGGAGGAA AGATACCGGC TTTGGATTCA GGATCCTGGG AGGAAATGAA 
CCTGGTGAGC CGATTTATAT TGGCCACATT GTGAAATATG GAGCTGCCGA TGAAGATGGG 
CGTCTGCGAT CAGGAGATGA GCTAATTTGC GTGGATGGAA CGGCCGTTGT TGGAAAATCT 
CATCAGCTCG TCGTTCAGCT CATGCAACAA GCTGCAAAAC AGGGTCACGT CAACCTGACC 
GTGAGGCGCA AGACTGGATA CGGACTGGCA AAGGTGGACG GTGAAATGCC TCCTTCTCCT 
GCCTCCTCTC ACCACAGCAG CACACAGGCC CCATCTCTGA CCGAGGAGAA GCGCACGCCA 
CAGGGCAGCC AGAACTCCCT AAACACGGTC AGCTCTGGGT CAGGCTCCAC CAGCGGTATC 
GGTAGTGGAG GTGGCGGCGG CAGCAACAAC ACAGTCATTC CCAATGCAAT CCTGCCCTAT 
GATGTGGAGA TCCAGCGCGG AGAGAATGAG GGATTTGGAT TTGTCATTGT GTCGTCGGTG 
TCGAGGCCTG AGGCGGGAAC AACTTTCGCT GGAAACGCAT GTGTGGCAAT GCCTCATAAA 
ATAGGTCGCA TCATCGAGGG AAGCCCAGCC GACCGCTGTG GAAAACTGAA AGTCGGAGAC 
CGGATCCTGG CTGTGAACGG CTGCTCCATC ACCAACAAGT CCCACTCGGA TATCGTCAAC 
CTGATCAAAG AGGCTGGAAA CACAGTGACG CTGCGGATCA TTCCAGGAGA CGAGTCCTCC 
AATGCATCAC TGCTGACCAA TGCTGAGAAG ATTGCAACCA TCACTACCAC ACACACACCT 
CAACAGCAAG CTGCACCTGA GCCAAGAAAC AACACCAAAC CAAAACAGGA GTCTTTTGAG 
TTCAAAGCCC CGCAAGCGCC GCCTCCTCCA GCTCCAACAC AATCTTCAAA TCAGGATGCT 
GAGTTTTACT CTGTGGATCT GGAGAGGGAC AATAAAGGCT TTGGTTTCAG CCTTAGAGGG 
GGTCGAGAGT ACAACATGGA CCTGTATGTG CTGAGACTCG CTGAGGACGG AGCGGCTGTC 
AGGAATGGGA AGATGAGGGT TGGAGATGAG ATCCTGGAGA TCAATGGAGA GAGCACCAAG 
GGCATGAAGC ACGCCCGCGC CATTGAACTG ATCAAAAACG GTGGCCGAAA AGTCCACCTG 
GTCCTAAGGA GGGGGGACGG CTCGGTGCCA GAATATGACG GCATCAACGA CGGCAACGGC 
CTCGGCGCCG GTGCACAAAC TGTTTCGGAA GTGAACTCAG CTCCTCCCGA AAGTCGGCCC 
CACCCACCAT CGGAGTCCAG CTATCCACCA GACCTTCACA AATCATCCCG AAATGACAAC 
AAAAGGACAC GGGAGTCAGG CCAACGGGAG CATGGTCACC GGTCAAACCA CCGCCACAAC 
AACCACAGAC ACCGCTCCCC TGAAAAGAAG AGCAGGAGCA AAGGGCACGG TAAGGACAGT 
GGAACTTCAA AGTCTCACAG GAAGAAGGGG GACAAGAAGA AATCTAGTAC GGATGATTCG 
CATCATCATC ACCACCACCA CAGCAACAGC CGCAACCAGT ACCACCATCA CCGCCAAAGG 
CACCGCTCCC CAGAGAGGCG CAGAACTCGA AGTGCCGGGG ACACCTTAGA CAGTAGACAC 
ACCCAGAGAA ACGGACGCTC ACCAGACAGG CGGCACCATC GCCACTCTTC CCCACGCAGA 
CACAGCTCAC CCCATCGCTC ACCTCACCGA TCTGGTTATC GCCGAAGCTC TCCTTATCGC 
AACAGATCCC CCTATCGCTC CCCTCAACGC TCCAGATACC AGTCTCCCTC AAGACGTCCC 
ACGGATAAAG AGTTCGACAG GGGAATTGAC GTGACCCTAA AGGAACCTCC TCCTCCGAAG 
CCCCCATCCC GCTCTGAGAG CATTCCACTT TTAGACCGGC TCAGTCTGGA AGACACTGTA 
ATGAGAGAAC CCCCCAGCTA CCGTCGTGAC CTTTCCCCAA TGGAGCGCGC CTATCGAGAG 
TCGACCCTTC TCCGAGGGGC TTCGCGTGAT GGCACCTTTC CCCGTGTTCG AACGCCAGAG 
TCAGATTCAG CTTTCCGGAA TTACAACTCA TTACTGAGGG GCCGTTCTCC AGTGAGAGTT 
GGGAACAGAC ACGATGCATA TCGCAGAGAT CAGTCTCCGG TTGCAAGCTC ATACCGAACA 
AGGAGTCTAG GTCGAGACCT GTCTCCCATT CGAGGGTTCC GCGATGAGGA AGAAGAGGAG 
GTTGAGTCTC AGCTGAGCGA GTATTATAGC CCGCTCCGAT CTAATTACAG CAGAGCTAAC 
AACAACGTAC CTGAGCCCAG GAGGAATGCC TACAGAGAAA GCCCCAAAGA TCTCAGCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304228.1
CDS	523..5265
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_009305953.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305953.3

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGAGGTAAG CCATTTTTCA CAAGGAACCC TGCAGAACTG 
AAGGGGACAT TTATTAACAC CAAGCTCAAG AAGAGCAGGC GTGGGTTCGG CTTCACTGTA 
GTAGGAGGAG ATGAACCCGA TGAGTTCTTG CAGATTAAGA GTCTTGTGCT CGATGGACCC 
GCTGCCCTTG ACGGAAAGAT GGAAACAGGT GATGTTATTG TAAGTGTGAA CGACACTTGC 
GTTTTGGGTT ACACACACGC CCAAGTTGTG AAGATCTTCC AGTCAATCCC TATTGGCTCT 
ATGGTCGACC TTGAACTCTG CCGTGGCTAC CCTTTGCCCT TTGACCCTGA TGACCCCAAC 
ACCAGCCTGG TGACTTCAGT GGCCATTCTG GACAAGGAGC CAATCATTGT CAATGGAAAA 
GAAAGCTACG ACTCACCATC CAGCCACGGG AGCGAAACTA CAGCTGGAGG CAGTAGCTTA 
AATGGCTCCA GTGATCCTCC TCGCCCCCAT AGCCCTTCAG CAGAGGTGGC TTCCAATGGC 
TCTCATGGTT ATCCCAGTGA TGTGGTGACT TTGGCGTCCT CGATCGCCAC CCAGCCCGAG 
CTTATTACTG TCCACATGGA AAAAGGCGAC AAGGGATTCG GCTTCACAAT AGCAGACAGC 
CCGTCTGGAG GAGGCCAGCG AGTAAAGCAG ATAGTGGATT ACCCACGATG CAGAGGCCTG 
AGGGAAGGTG ACATCATCAT GGAGGTGAAC AAGAGGAACG TGCAGAACCT GACGCACAAT 
CAGGTGGTCG ACCTGCTCAG CAAGTGTCCT AAAGGCAGTG AAGTGACCAT GTTGGTGCAA 
CGAGGGGTGG CACCTGCGAA GAAGAGCCCC AAACTGCAAC AGTTGGAGCG CAAGGACAGT 
CAGGGCAGCT CCCAGCACAG CGTGTCCAGT CAGCGCAGCA TCCACACAGA CTCTCCTCTC 
CACGCCTCTC AGGCCCTGCT CAACGAGTCG GCCGCCCCAC CACCCCCGTC CCAGCCCTTG 
CCCAGCCTGC CCCAGGACCC TCCAGTAGAC GGAACCCTCC AAAAGAAGCC AGATCCCTTT 
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAGTAGGC TGCCTGATTA CCAGGAGCAG 
GATATCTTCC TGTGGAGGAA AGATACCGGC TTTGGATTCA GGATCCTGGG AGGAAATGAA 
CCTGGTGAGC CGATTTATAT TGGCCACATT GTGAAATATG GAGCTGCCGA TGAAGATGGG 
CGTCTGCGAT CAGGAGATGA GCTAATTTGC GTGGATGGAA CGGCCGTTGT TGGAAAATCT 
CATCAGCTCG TCGTTCAGCT CATGCAACAA GCTGCAAAAC AGGGTCACGT CAACCTGACC 
GTGAGGCGCA AGACTGGATA CGGACTGGCA AAGGTGGACG GTGAAATGCC TCCTTCTCCT 
GCCTCCTCTC ACCACAGCAG CACACAGGCC CCATCTCTGA CCGAGGAGAA GCGCACGCCA 
CAGGGCAGCC AGAACTCCCT AAACACGGTC AGCTCTGGGT CAGGCTCCAC CAGCGGTATC 
GGTAGTGGAG GTGGCGGCGG CAGCAACAAC ACAGTCATTC CCAATGCAAT CCTGCCCTAT 
GATGTGGAGA TCCAGCGCGG AGAGAATGAG GGATTTGGAT TTGTCATTGT GTCGTCGGTG 
TCGAGGCCTG AGGCGGGAAC AACTTTCGCT GGAAACGCAT GTGTGGCAAT GCCTCATAAA 
ATAGGTCGCA TCATCGAGGG AAGCCCAGCC GACCGCTGTG GAAAACTGAA AGTCGGAGAC 
CGGATCCTGG CTGTGAACGG CTGCTCCATC ACCAACAAGT CCCACTCGGA TATCGTCAAC 
CTGATCAAAG AGGCTGGAAA CACAGTGACG CTGCGGATCA TTCCAGGAGA CGAGTCCTCC 
AATGCATCAC TGCTGACCAA TGCTGAGAAG ATTGCAACCA TCACTACCAC ACACACACCT 
CAACAGCAAG CTGCACCTGA GCCAAGAAAC AACACCAAAC CAAAACAGGA GTCTTTTGAG 
TTCAAAGCCC CGCAAGCGCC GCCTCCTCCA GCTCCAACAC AATCTTCAAA TCAGGATGCT 
GAGTTTTACT CTGTGGATCT GGAGAGGGAC AATAAAGGCT TTGGTTTCAG CCTTAGAGGG 
GGTCGAGAGT ACAACATGGA CCTGTATGTG CTGAGACTCG CTGAGGACGG AGCGGCTGTC 
AGGAATGGGA AGATGAGGGT TGGAGATGAG ATCCTGGAGA TCAATGGAGA GAGCACCAAG 
GGCATGAAGC ACGCCCGCGC CATTGAACTG ATCAAAAACG GTGGCCGAAA AGTCCACCTG 
GTCCTAAGGA GGGGGGACGG CTCGGTGCCA GAATATGACG GCATCAACGA CGGCAACGGC 
CTCGGCGCCG GTGCACAAAC TGTTTCGGAA GTGAACTCAG CTCCTCCCGA AAGTCGGCCC 
CACCCACCAT CGGAGTCCAG CTATCCACCA GACCTTCACA AATCATCCCG AAATGACAAC 
AAAAGGACAC GGGAGTCAGG CCAACGGGAG CATGGTCACC GGTCAAACCA CCGCCACAAC 
AACCACAGAC ACCGCTCCCC TGAAAAGAAG AGCAGGAGCA AAGGGCACGG TAAGGACAGT 
GGAACTTCAA AGTCTCACAG GAAGAAGGGG GACAAGAAGA AATCTAGTAC GGATGATTCG 
CATCATCATC ACCACCACCA CAGCAACAGC CGCAACCAGT ACCACCATCA CCGCCAAAGG 
CACCGCTCCC CAGAGAGGCG CAGAACTCGA AGTGCCGGGG ACACCTTAGA CAGTAGACAC 
ACCCAGAGAA ACGGACGCTC ACCAGACAGG CGGCACCATC GCCACTCTTC CCCACGCAGA 
CACAGCTCAC CCCATCGCTC ACCTCACCGA TCTGGTTATC GCCGAAGCTC TCCTTATCGC 
AACAGATCCC CCTATCGCTC CCCTCAACGC TCCAGATACC AGTCTCCCTC AAGACGTCCC 
ACGGATAAAG AGTTCGACAG GGGAATTGAC GTGACCCTAA AGGAACCTCC TCCTCCGAAG 
CCCCCATCCC GCTCTGAGAG CATTCCACTT TTAGACCGGC TCAGTCTGGA AGACACTGTA 
ATGAGAGAAC CCCCCAGCTA CCGTCGTGAC CTTTCCCCAA TGGAGCGCGC CTATCGAGAG 
TCGACCCTTC TCCGAGGGGC TTCGCGTGAT GGCACCTTTC CCCGTGTTCG AACGCCAGAG 
TCAGATTCAG CTTTCCGGAA TTACAACTCA TTACTGAGGG GCCGTTCTCC AGTGAGAGTT 
GGGAACAGAC ACGATGCATA TCGCAGAGAT CAGTCTCCGG TTGCAAGCTC ATACCGAACA 
AGGAGTCTAG GTCGAGACCT GTCTCCCATT CGAGGGTTCC GCGATGAGGA AGAAGAGGAG 
GTTGAGTCTC AGCTGAGCGA GTATTATAGC CCGCTCCGAT CTAATTACAG CAGAGCTAAC 
AACAACGTAC CTGAGCCCAG GAGGAATGCC TACAGAGAAA GCCCCAAAGA TCTCAGCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305953.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304228.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.

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CloneID	ODa41342
Clone ID Related Accession (Same CDS sequence)	XM_009305953.3 , XM_009305953.2
Accession Version	XM_009305953.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGAGGTAAG CCATTTTTCA CAAGGAACCC TGCAGAACTG 
AAGGGGACAT TTATTAACAC CAAGCTCAAG AAGAGCAGGC GTGGGTTCGG CTTCACTGTA 
GTAGGAGGAG ATGAACCCGA TGAGTTCTTG CAGATTAAGA GTCTTGTGCT CGATGGACCC 
GCTGCCCTTG ACGGAAAGAT GGAAACAGGT GATGTTATTG TAAGTGTGAA CGACACTTGC 
GTTTTGGGTT ACACACACGC CCAAGTTGTG AAGATCTTCC AGTCAATCCC TATTGGCTCT 
ATGGTCGACC TTGAACTCTG CCGTGGCTAC CCTTTGCCCT TTGACCCTGA TGACCCCAAC 
ACCAGCCTGG TGACTTCAGT GGCCATTCTG GACAAGGAGC CAATCATTGT CAATGGAAAA 
GAAAGCTACG ACTCACCATC CAGCCACGGG AGCGAAACTA CAGCTGGAGG CAGTAGCTTA 
AATGGCTCCA GTGATCCTCC TCGCCCCCAT AGCCCTTCAG CAGAGGTGGC TTCCAATGGC 
TCTCATGGTT ATCCCAGTGA TGTGGTGACT TTGGCGTCCT CGATCGCCAC CCAGCCCGAG 
CTTATTACTG TCCACATGGA AAAAGGCGAC AAGGGATTCG GCTTCACAAT AGCAGACAGC 
CCGTCTGGAG GAGGCCAGCG AGTAAAGCAG ATAGTGGATT ACCCACGATG CAGAGGCCTG 
AGGGAAGGTG ACATCATCAT GGAGGTGAAC AAGAGGAACG TGCAGAACCT GACGCACAAT 
CAGGTGGTCG ACCTGCTCAG CAAGTGTCCT AAAGGCAGTG AAGTGACCAT GTTGGTGCAA 
CGAGGGGTGG CACCTGCGAA GAAGAGCCCC AAACTGCAAC AGTTGGAGCG CAAGGACAGT 
CAGGGCAGCT CCCAGCACAG CGTGTCCAGT CAGCGCAGCA TCCACACAGA CTCTCCTCTC 
CACGCCTCTC AGGCCCTGCT CAACGAGTCG GCCGCCCCAC CACCCCCGTC CCAGCCCTTG 
CCCAGCCTGC CCCAGGACCC TCCAGTAGAC GGAACCCTCC AAAAGAAGCC AGATCCCTTT 
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAGTAGGC TGCCTGATTA CCAGGAGCAG 
GATATCTTCC TGTGGAGGAA AGATACCGGC TTTGGATTCA GGATCCTGGG AGGAAATGAA 
CCTGGTGAGC CGATTTATAT TGGCCACATT GTGAAATATG GAGCTGCCGA TGAAGATGGG 
CGTCTGCGAT CAGGAGATGA GCTAATTTGC GTGGATGGAA CGGCCGTTGT TGGAAAATCT 
CATCAGCTCG TCGTTCAGCT CATGCAACAA GCTGCAAAAC AGGGTCACGT CAACCTGACC 
GTGAGGCGCA AGACTGGATA CGGACTGGCA AAGGTGGACG GTGAAATGCC TCCTTCTCCT 
GCCTCCTCTC ACCACAGCAG CACACAGGCC CCATCTCTGA CCGAGGAGAA GCGCACGCCA 
CAGGGCAGCC AGAACTCCCT AAACACGGTC AGCTCTGGGT CAGGCTCCAC CAGCGGTATC 
GGTAGTGGAG GTGGCGGCGG CAGCAACAAC ACAGTCATTC CCAATGCAAT CCTGCCCTAT 
GATGTGGAGA TCCAGCGCGG AGAGAATGAG GGATTTGGAT TTGTCATTGT GTCGTCGGTG 
TCGAGGCCTG AGGCGGGAAC AACTTTCGCT GGAAACGCAT GTGTGGCAAT GCCTCATAAA 
ATAGGTCGCA TCATCGAGGG AAGCCCAGCC GACCGCTGTG GAAAACTGAA AGTCGGAGAC 
CGGATCCTGG CTGTGAACGG CTGCTCCATC ACCAACAAGT CCCACTCGGA TATCGTCAAC 
CTGATCAAAG AGGCTGGAAA CACAGTGACG CTGCGGATCA TTCCAGGAGA CGAGTCCTCC 
AATGCATCAC TGCTGACCAA TGCTGAGAAG ATTGCAACCA TCACTACCAC ACACACACCT 
CAACAGCAAG CTGCACCTGA GCCAAGAAAC AACACCAAAC CAAAACAGGA GTCTTTTGAG 
TTCAAAGCCC CGCAAGCGCC GCCTCCTCCA GCTCCAACAC AATCTTCAAA TCAGGATGCT 
GAGTTTTACT CTGTGGATCT GGAGAGGGAC AATAAAGGCT TTGGTTTCAG CCTTAGAGGG 
GGTCGAGAGT ACAACATGGA CCTGTATGTG CTGAGACTCG CTGAGGACGG AGCGGCTGTC 
AGGAATGGGA AGATGAGGGT TGGAGATGAG ATCCTGGAGA TCAATGGAGA GAGCACCAAG 
GGCATGAAGC ACGCCCGCGC CATTGAACTG ATCAAAAACG GTGGCCGAAA AGTCCACCTG 
GTCCTAAGGA GGGGGGACGG CTCGGTGCCA GAATATGACG GCATCAACGA CGGCAACGGC 
CTCGGCGCCG GTGCACAAAC TGTTTCGGAA GTGAACTCAG CTCCTCCCGA AAGTCGGCCC 
CACCCACCAT CGGAGTCCAG CTATCCACCA GACCTTCACA AATCATCCCG AAATGACAAC 
AAAAGGACAC GGGAGTCAGG CCAACGGGAG CATGGTCACC GGTCAAACCA CCGCCACAAC 
AACCACAGAC ACCGCTCCCC TGAAAAGAAG AGCAGGAGCA AAGGGCACGG TAAGGACAGT 
GGAACTTCAA AGTCTCACAG GAAGAAGGGG GACAAGAAGA AATCTAGTAC GGATGATTCG 
CATCATCATC ACCACCACCA CAGCAACAGC CGCAACCAGT ACCACCATCA CCGCCAAAGG 
CACCGCTCCC CAGAGAGGCG CAGAACTCGA AGTGCCGGGG ACACCTTAGA CAGTAGACAC 
ACCCAGAGAA ACGGACGCTC ACCAGACAGG CGGCACCATC GCCACTCTTC CCCACGCAGA 
CACAGCTCAC CCCATCGCTC ACCTCACCGA TCTGGTTATC GCCGAAGCTC TCCTTATCGC 
AACAGATCCC CCTATCGCTC CCCTCAACGC TCCAGATACC AGTCTCCCTC AAGACGTCCC 
ACGGATAAAG AGTTCGACAG GGGAATTGAC GTGACCCTAA AGGAACCTCC TCCTCCGAAG 
CCCCCATCCC GCTCTGAGAG CATTCCACTT TTAGACCGGC TCAGTCTGGA AGACACTGTA 
ATGAGAGAAC CCCCCAGCTA CCGTCGTGAC CTTTCCCCAA TGGAGCGCGC CTATCGAGAG 
TCGACCCTTC TCCGAGGGGC TTCGCGTGAT GGCACCTTTC CCCGTGTTCG AACGCCAGAG 
TCAGATTCAG CTTTCCGGAA TTACAACTCA TTACTGAGGG GCCGTTCTCC AGTGAGAGTT 
GGGAACAGAC ACGATGCATA TCGCAGAGAT CAGTCTCCGG TTGCAAGCTC ATACCGAACA 
AGGAGTCTAG GTCGAGACCT GTCTCCCATT CGAGGGTTCC GCGATGAGGA AGAAGAGGAG 
GTTGAGTCTC AGCTGAGCGA GTATTATAGC CCGCTCCGAT CTAATTACAG CAGAGCTAAC 
AACAACGTAC CTGAGCCCAG GAGGAATGCC TACAGAGAAA GCCCCAAAGA TCTCAGCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304228.1
CDS	518..5260
Translation	MSKVVQKKNHWTTKVNECSVSKDARGELNVQLLGGAENGEFAYIGHVNETAVQYRNGKVSEGELLLEVETLSVSGLPLYDVLTVIKNCKGPIRLKTVRQGHKLNKDLKHYLSQRFQKSSADYELQQTIRDNLYRHAVPCTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_009305953.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305953.2

ORF Insert Sequence:

ATGTCCAAAG TGGTACAGAA GAAGAATCAC TGGACCACCA AAGTTAACGA GTGCTCGGTC 
TCCAAGGACG CCCGTGGCGA GCTGAACGTG CAGCTGCTCG GTGGCGCGGA GAACGGAGAG 
TTCGCCTATA TCGGACACGT TAACGAGACC GCCGTACAGT ATAGAAATGG GAAAGTGTCC 
GAGGGGGAAT TACTGCTGGA AGTGGAGACG CTTTCTGTCT CTGGATTGCC GCTGTATGAT 
GTGCTGACAG TAATCAAGAA CTGCAAAGGA CCTATAAGGT TAAAAACTGT GAGGCAAGGA 
CATAAACTCA ATAAAGACCT GAAGCACTAC CTGAGCCAGA GGTTTCAGAA ATCCTCCGCC 
GATTATGAGC TTCAGCAAAC CATACGAGAC AACCTCTACC GCCACGCTGT GCCTTGTACA 
ACTCGCCCCC CTCGTGAAGG AGAGGTGCCG GGAGTAGATT ACAATTTCTT GTCAGTGGAC 
GAGTTTCTGA AGCTTGAACA GAGTGGGACA CTGTTGGAGA TCGGATCTTA CGAAGGTAAC 
TATTATGGCA CACCCAAGCC GCCTATTAAG CCCCCTAGTG GCAAAGTGAT CACTGGCGAT 
GCTCTTCAAG ACAGCCTTCC CGGATCCCAG CCGTCCACCC CGCGTCGCAC CAAGTCCTAC 
AATGAGATGC AAAATGCTGG AATAGTGCCT ACAGAGACTG AGGATGATGA CGAGGTTCCT 
GAAATGAACA GCAGCTTCAC AGGAGACTCG AGCGAACCGG ACGATCACCC CTCCGTGAGG 
CCCTTCGCCC GCGACCAACT TCAACCCTAC GGGCTCAACA ACACCTCGGC GTCAACCACG 
GAGAGCTCTC AGAACACACA CACACACCCC GCCTACCCAA CGGAGGAGGA CCCACTGGGC 
CCTCTGCCTG AAAACTGGGA GATGGCGTAC ACGGAGAATG GAGAGGTCTA CTTTATAGAC 
CACAACACAA AGACAACATC CTGGATCGAC CCTCGATGCC TGGACAAACC TCAGAAACCC 
CTGGAGGAAT GTGAAGATGA TGAAGGGGTC CATACAGAGG AGCTGGACAA TGAACTAGAG 
CTGCCAACAG GCTGGGAGAA GATCGATGAT CCAGTGTACG GGGTGTATTA TGTTGATCAT 
ATTAACAGGA AGACGCAGTA TGAGAACCCA ATTCTGGAAG CTAAAAGGAA GAAGCAACTG 
GAGCAGTCTC AGCCCGCTGA AGGAGGTAAG CCATTTTTCA CAAGGAACCC TGCAGAACTG 
AAGGGGACAT TTATTAACAC CAAGCTCAAG AAGAGCAGGC GTGGGTTCGG CTTCACTGTA 
GTAGGAGGAG ATGAACCCGA TGAGTTCTTG CAGATTAAGA GTCTTGTGCT CGATGGACCC 
GCTGCCCTTG ACGGAAAGAT GGAAACAGGT GATGTTATTG TAAGTGTGAA CGACACTTGC 
GTTTTGGGTT ACACACACGC CCAAGTTGTG AAGATCTTCC AGTCAATCCC TATTGGCTCT 
ATGGTCGACC TTGAACTCTG CCGTGGCTAC CCTTTGCCCT TTGACCCTGA TGACCCCAAC 
ACCAGCCTGG TGACTTCAGT GGCCATTCTG GACAAGGAGC CAATCATTGT CAATGGAAAA 
GAAAGCTACG ACTCACCATC CAGCCACGGG AGCGAAACTA CAGCTGGAGG CAGTAGCTTA 
AATGGCTCCA GTGATCCTCC TCGCCCCCAT AGCCCTTCAG CAGAGGTGGC TTCCAATGGC 
TCTCATGGTT ATCCCAGTGA TGTGGTGACT TTGGCGTCCT CGATCGCCAC CCAGCCCGAG 
CTTATTACTG TCCACATGGA AAAAGGCGAC AAGGGATTCG GCTTCACAAT AGCAGACAGC 
CCGTCTGGAG GAGGCCAGCG AGTAAAGCAG ATAGTGGATT ACCCACGATG CAGAGGCCTG 
AGGGAAGGTG ACATCATCAT GGAGGTGAAC AAGAGGAACG TGCAGAACCT GACGCACAAT 
CAGGTGGTCG ACCTGCTCAG CAAGTGTCCT AAAGGCAGTG AAGTGACCAT GTTGGTGCAA 
CGAGGGGTGG CACCTGCGAA GAAGAGCCCC AAACTGCAAC AGTTGGAGCG CAAGGACAGT 
CAGGGCAGCT CCCAGCACAG CGTGTCCAGT CAGCGCAGCA TCCACACAGA CTCTCCTCTC 
CACGCCTCTC AGGCCCTGCT CAACGAGTCG GCCGCCCCAC CACCCCCGTC CCAGCCCTTG 
CCCAGCCTGC CCCAGGACCC TCCAGTAGAC GGAACCCTCC AAAAGAAGCC AGATCCCTTT 
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAGTAGGC TGCCTGATTA CCAGGAGCAG 
GATATCTTCC TGTGGAGGAA AGATACCGGC TTTGGATTCA GGATCCTGGG AGGAAATGAA 
CCTGGTGAGC CGATTTATAT TGGCCACATT GTGAAATATG GAGCTGCCGA TGAAGATGGG 
CGTCTGCGAT CAGGAGATGA GCTAATTTGC GTGGATGGAA CGGCCGTTGT TGGAAAATCT 
CATCAGCTCG TCGTTCAGCT CATGCAACAA GCTGCAAAAC AGGGTCACGT CAACCTGACC 
GTGAGGCGCA AGACTGGATA CGGACTGGCA AAGGTGGACG GTGAAATGCC TCCTTCTCCT 
GCCTCCTCTC ACCACAGCAG CACACAGGCC CCATCTCTGA CCGAGGAGAA GCGCACGCCA 
CAGGGCAGCC AGAACTCCCT AAACACGGTC AGCTCTGGGT CAGGCTCCAC CAGCGGTATC 
GGTAGTGGAG GTGGCGGCGG CAGCAACAAC ACAGTCATTC CCAATGCAAT CCTGCCCTAT 
GATGTGGAGA TCCAGCGCGG AGAGAATGAG GGATTTGGAT TTGTCATTGT GTCGTCGGTG 
TCGAGGCCTG AGGCGGGAAC AACTTTCGCT GGAAACGCAT GTGTGGCAAT GCCTCATAAA 
ATAGGTCGCA TCATCGAGGG AAGCCCAGCC GACCGCTGTG GAAAACTGAA AGTCGGAGAC 
CGGATCCTGG CTGTGAACGG CTGCTCCATC ACCAACAAGT CCCACTCGGA TATCGTCAAC 
CTGATCAAAG AGGCTGGAAA CACAGTGACG CTGCGGATCA TTCCAGGAGA CGAGTCCTCC 
AATGCATCAC TGCTGACCAA TGCTGAGAAG ATTGCAACCA TCACTACCAC ACACACACCT 
CAACAGCAAG CTGCACCTGA GCCAAGAAAC AACACCAAAC CAAAACAGGA GTCTTTTGAG 
TTCAAAGCCC CGCAAGCGCC GCCTCCTCCA GCTCCAACAC AATCTTCAAA TCAGGATGCT 
GAGTTTTACT CTGTGGATCT GGAGAGGGAC AATAAAGGCT TTGGTTTCAG CCTTAGAGGG 
GGTCGAGAGT ACAACATGGA CCTGTATGTG CTGAGACTCG CTGAGGACGG AGCGGCTGTC 
AGGAATGGGA AGATGAGGGT TGGAGATGAG ATCCTGGAGA TCAATGGAGA GAGCACCAAG 
GGCATGAAGC ACGCCCGCGC CATTGAACTG ATCAAAAACG GTGGCCGAAA AGTCCACCTG 
GTCCTAAGGA GGGGGGACGG CTCGGTGCCA GAATATGACG GCATCAACGA CGGCAACGGC 
CTCGGCGCCG GTGCACAAAC TGTTTCGGAA GTGAACTCAG CTCCTCCCGA AAGTCGGCCC 
CACCCACCAT CGGAGTCCAG CTATCCACCA GACCTTCACA AATCATCCCG AAATGACAAC 
AAAAGGACAC GGGAGTCAGG CCAACGGGAG CATGGTCACC GGTCAAACCA CCGCCACAAC 
AACCACAGAC ACCGCTCCCC TGAAAAGAAG AGCAGGAGCA AAGGGCACGG TAAGGACAGT 
GGAACTTCAA AGTCTCACAG GAAGAAGGGG GACAAGAAGA AATCTAGTAC GGATGATTCG 
CATCATCATC ACCACCACCA CAGCAACAGC CGCAACCAGT ACCACCATCA CCGCCAAAGG 
CACCGCTCCC CAGAGAGGCG CAGAACTCGA AGTGCCGGGG ACACCTTAGA CAGTAGACAC 
ACCCAGAGAA ACGGACGCTC ACCAGACAGG CGGCACCATC GCCACTCTTC CCCACGCAGA 
CACAGCTCAC CCCATCGCTC ACCTCACCGA TCTGGTTATC GCCGAAGCTC TCCTTATCGC 
AACAGATCCC CCTATCGCTC CCCTCAACGC TCCAGATACC AGTCTCCCTC AAGACGTCCC 
ACGGATAAAG AGTTCGACAG GGGAATTGAC GTGACCCTAA AGGAACCTCC TCCTCCGAAG 
CCCCCATCCC GCTCTGAGAG CATTCCACTT TTAGACCGGC TCAGTCTGGA AGACACTGTA 
ATGAGAGAAC CCCCCAGCTA CCGTCGTGAC CTTTCCCCAA TGGAGCGCGC CTATCGAGAG 
TCGACCCTTC TCCGAGGGGC TTCGCGTGAT GGCACCTTTC CCCGTGTTCG AACGCCAGAG 
TCAGATTCAG CTTTCCGGAA TTACAACTCA TTACTGAGGG GCCGTTCTCC AGTGAGAGTT 
GGGAACAGAC ACGATGCATA TCGCAGAGAT CAGTCTCCGG TTGCAAGCTC ATACCGAACA 
AGGAGTCTAG GTCGAGACCT GTCTCCCATT CGAGGGTTCC GCGATGAGGA AGAAGAGGAG 
GTTGAGTCTC AGCTGAGCGA GTATTATAGC CCGCTCCGAT CTAATTACAG CAGAGCTAAC 
AACAACGTAC CTGAGCCCAG GAGGAATGCC TACAGAGAAA GCCCCAAAGA TCTCAGCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1b ( XM_009305954.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304229.1 Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.

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CloneID	ODa41343
Clone ID Related Accession (Same CDS sequence)	XM_009305954.2
Accession Version	XM_009305954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC TGTTTACACT GAGACGGTGG AAGCGGGGAT TCGGTCAATA TTCACAAAAA 
TCCTCCTTTC GATTGAGTAC AACTCGCCCC CCTCGTGAAG GAGAGGTGCC GGGAGTAGAT 
TACAATTTCT TGTCAGTGGA CGAGTTTCTG AAGCTTGAAC AGAGTGGGAC ACTGTTGGAG 
ATCGGATCTT ACGAAGGTAA CTATTATGGC ACACCCAAGC CGCCTATTAA GCCCCCTAGT 
GGCAAAGTGA TCACTGGCGA TGCTCTTCAA GACAGCCTTC CCGGATCCCA GCCGTCCACC 
CCGCGTCGCA CCAAGTCCTA CAATGAGATG CAAAATGCTG GAATAGTGCC TACAGAGACT 
GAGGATGATG ACGAGGTTCC TGAAATGAAC AGCAGCTTCA CAGGAGACTC GAGCGAACCG 
GACGATCACC CCTCCGTGAG GCCCTTCGCC CGCGACCAAC TTCAACCCTA CGGGCTCAAC 
AACACCTCGG CGTCAACCAC GGAGAGCTCT CAGAACACAC ACACACACCC CGCCTACCCA 
ACGGAGGAGG ACCCACTGGG CCCTCTGCCT GAAAACTGGG AGATGGCGTA CACGGAGAAT 
GGAGAGGTCT ACTTTATAGA CCACAACACA AAGACAACAT CCTGGATCGA CCCTCGATGC 
CTGGACAAAC CTCAGAAACC CCTGGAGGAA TGTGAAGATG ATGAAGGGGT CCATACAGAG 
GAGCTGGACA ATGAACTAGA GCTGCCAACA GGCTGGGAGA AGATCGATGA TCCAGTGTAC 
GGGGTGTATT ATGTTGATCA TATTAACAGG AAGACGCAGT ATGAGAACCC AATTCTGGAA 
GCTAAAAGGA AGAAGCAACT GGAGCAGTCT CAGCCCGCTG AAGGTGAGCG GTTTATCCAA 
GAATGGATAG ACGAGCATTC GTCAGGAGCA CCGCTAGCGA GCTATGCTGC TAACCACCAA 
GAGACTTACA GAGACCCCGG CACCGGCCCT CCACTACCAA CTCAACTGGG GGCTAAACGA 
GGTAAGCCAT TTTTCACAAG GAACCCTGCA GAACTGAAGG GGACATTTAT TAACACCAAG 
CTCAAGAAGA GCAGGCGTGG GTTCGGCTTC ACTGTAGTAG GAGGAGATGA ACCCGATGAG 
TTCTTGCAGA TTAAGAGTCT TGTGCTCGAT GGACCCGCTG CCCTTGACGG AAAGATGGAA 
ACAGGTGATG TTATTGTAAG TGTGAACGAC ACTTGCGTTT TGGGTTACAC ACACGCCCAA 
GTTGTGAAGA TCTTCCAGTC AATCCCTATT GGCTCTATGG TCGACCTTGA ACTCTGCCGT 
GGCTACCCTT TGCCCTTTGA CCCTGATGAC CCCAACACCA GCCTGGTGAC TTCAGTGGCC 
ATTCTGGACA AGGAGCCAAT CATTGTCAAT GGAAAAGAAA GCTACGACTC ACCATCCAGC 
CACGGGAGCG AAACTACAGC TGGAGGCAGT AGCTTAAATG GCTCCAGTGA TCCTCCTCGC 
CCCCATAGCC CTTCAGCAGA GGTGGCTTCC AATGGCTCTC ATGGTTATCC CAGTGATGTG 
GTGACTTTGG CGTCCTCGAT CGCCACCCAG CCCGAGCTTA TTACTGTCCA CATGGAAAAA 
GGCGACAAGG GATTCGGCTT CACAATAGCA GACAGCCCGT CTGGAGGAGG CCAGCGAGTA 
AAGCAGATAG TGGATTACCC ACGATGCAGA GGCCTGAGGG AAGGTGACAT CATCATGGAG 
GTGAACAAGA GGAACGTGCA GAACCTGACG CACAATCAGG TGGTCGACCT GCTCAGCAAG 
TGTCCTAAAG GCAGTGAAGT GACCATGTTG GTGCAACGAG GGGTGGCACC TGCGAAGAAG 
AGCCCCAAAC TGCAACAGTT GGAGCGCAAG GACAGTCAGG GCAGCTCCCA GCACAGCGTG 
TCCAGTCAGC GCAGCATCCA CACAGACTCT CCTCTCCACG CCTCTCAGGC CCTGCTCAAC 
GAGTCGGCCG CCCCACCACC CCCGTCCCAG CCCTTGCCCA GCCTGCCCCA GGACCCTCCA 
GTAGACGGAA CCCTCCAAAA GAAGCCAGAT CCCTTTAAAA TCTGGGCCCA GTCCAGGAGC 
ATGTATGAAA GTAGGCTGCC TGATTACCAG GAGCAGGATA TCTTCCTGTG GAGGAAAGAT 
ACCGGCTTTG GATTCAGGAT CCTGGGAGGA AATGAACCTG GTGAGCCGAT TTATATTGGC 
CACATTGTGA AATATGGAGC TGCCGATGAA GATGGGCGTC TGCGATCAGG AGATGAGCTA 
ATTTGCGTGG ATGGAACGGC CGTTGTTGGA AAATCTCATC AGCTCGTCGT TCAGCTCATG 
CAACAAGCTG CAAAACAGGG TCACGTCAAC CTGACCGTGA GGCGCAAGAC TGGATACGGA 
CTGGCAAAGG TGGACGGTGA AATGCCTCCT TCTCCTGCCT CCTCTCACCA CAGCAGCACA 
CAGGCCCCAT CTCTGACCGA GGAGAAGCGC ACGCCACAGG GCAGCCAGAA CTCCCTAAAC 
ACGGTCAGCT CTGGGTCAGG CTCCACCAGC GGTATCGGTA GTGGAGGTGG CGGCGGCAGC 
AACAACACAG TCATTCCCAA TGCAATCCTG CCCTATGATG TGGAGATCCA GCGCGGAGAG 
AATGAGGGAT TTGGATTTGT CATTGTGTCG TCGGTGTCGA GGCCTGAGGC GGGAACAACT 
TTCGCTGGAA ACGCATGTGT GGCAATGCCT CATAAAATAG GTCGCATCAT CGAGGGAAGC 
CCAGCCGACC GCTGTGGAAA ACTGAAAGTC GGAGACCGGA TCCTGGCTGT GAACGGCTGC 
TCCATCACCA ACAAGTCCCA CTCGGATATC GTCAACCTGA TCAAAGAGGC TGGAAACACA 
GTGACGCTGC GGATCATTCC AGGAGACGAG TCCTCCAATG CATCACTGCT GACCAATGCT 
GAGAAGATTG CAACCATCAC TACCACACAC ACACCTCAAC AGCAAGCTGC ACCTGAGCCA 
AGAAACAACA CCAAACCAAA ACAGGAGTCT TTTGAGTTCA AAGCCCCGCA AGCGCCGCCT 
CCTCCAGCTC CAACACAATC TTCAAATCAG GATGCTGAGT TTTACTCTGT GGATCTGGAG 
AGGGACAATA AAGGCTTTGG TTTCAGCCTT AGAGGGGGTC GAGAGTACAA CATGGACCTG 
TATGTGCTGA GACTCGCTGA GGACGGAGCG GCTGTCAGGA ATGGGAAGAT GAGGGTTGGA 
GATGAGATCC TGGAGATCAA TGGAGAGAGC ACCAAGGGCA TGAAGCACGC CCGCGCCATT 
GAACTGATCA AAAACGGTGG CCGAAAAGTC CACCTGGTCC TAAGGAGGGG GGACGGCTCG 
GTGCCAGAAT ATGACGGCAT CAACGACGGC AACGGCCTCG GCGCCGGTGC ACAAACTGTT 
TCGGAAGTGA ACTCAGCTCC TCCCGAAAGT CGGCCCCACC CACCATCGGA GTCCAGCTAT 
CCACCAGACC TTCACAAATC ATCCCGAAAT GACAACAAAA GGACACGGGA GTCAGGCCAA 
CGGGAGCATG GTCACCGGTC AAACCACCGC CACAACAACC ACAGACACCG CTCCCCTGAA 
AAGAAGAGCA GGAGCAAAGG GCACGGTAAG GACAGTGGAA CTTCAAAGTC TCACAGGAAG 
AAGGGGGACA AGAAGAAATC TAGTACGGAT GATTCGCATC ATCATCACCA CCACCACAGC 
AACAGCCGCA ACCAGTACCA CCATCACCGC CAAAGGCACC GCTCCCCAGA GAGGCGCAGA 
ACTCGAAGTG CCGGGGACAC CTTAGACAGT AGACACACCC AGAGAAACGG ACGCTCACCA 
GACAGGCGGC ACCATCGCCA CTCTTCCCCA CGCAGACACA GCTCACCCCA TCGCTCACCT 
CACCGATCTG GTTATCGCCG AAGCTCTCCT TATCGCAACA GATCCCCCTA TCGCTCCCCT 
CAACGCTCCA GATACCAGTC TCCCTCAAGA CGTCCCACGG ATAAAGAGTT CGACAGGGGA 
ATTGACGTGA CCCTAAAGGA ACCTCCTCCT CCGAAGCCCC CATCCCGCTC TGAGAGCATT 
CCACTTTTAG ACCGGCTCAG TCTGGAAGAC ACTGTAATGA GAGAACCCCC CAGCTACCGT 
CGTGACCTTT CCCCAATGGA GCGCGCCTAT CGAGAGTCGA CCCTTCTCCG AGGGGCTTCG 
CGTGATGGCA CCTTTCCCCG TGTTCGAACG CCAGAGTCAG ATTCAGCTTT CCGGAATTAC 
AACTCATTAC TGAGGGGCCG TTCTCCAGTG AGAGTTGGGA ACAGACACGA TGCATATCGC 
AGAGATCAGT CTCCGGTTGC AAGCTCATAC CGAACAAGGA GTCTAGGTCG AGACCTGTCT 
CCCATTCGAG GGTTCCGCGA TGAGGAAGAA GAGGAGGTTG AGTCTCAGCT GAGCGAGTAT 
TATAGCCCGC TCCGATCTAA TTACAGCAGA GCTAACAACA ACGTACCTGA GCCCAGGAGG 
AATGCCTACA GAGAAAGCCC CAAAGATCTC AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304229.1
CDS	11..4549
Translation	MAGLFTLRRWKRGFGQYSQKSSFRLSTTRPPREGEVPGVDYNFLSVDEFLKLEQSGTLLEIGSYEGNYYGTPKPPIKPPSGKVITGDALQDSLPGSQPSTPRRTKSYNEMQNAGIVPTETEDDDEVPEMNSSFTGDSSEPDDHPSVRPFARDQLQPYGLNNTSASTTESSQNTHTHPAYPTEEDPLGPLPENWEMAYTENGEVYFIDHNTKTTSWIDPRCLDKPQKPLEECEDDEGVHTEELDNELELPTGWEKIDDPVYGVYYVDHINRKTQYENPILEAKRKKQLEQSQPAEGERFIQEWIDEHSSGAPLASYAANHQETYRDPGTGPPLPTQLGAKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGYTHAQVVKIFQSIPIGSMVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGKESYDSPSSHGSETTAGGSSLNGSSDPPRPHSPSAEVASNGSHGYPSDVVTLASSIATQPELITVHMEKGDKGFGFTIADSPSGGGQRVKQIVDYPRCRGLREGDIIMEVNKRNVQNLTHNQVVDLLSKCPKGSEVTMLVQRGVAPAKKSPKLQQLERKDSQGSSQHSVSSQRSIHTDSPLHASQALLNESAAPPPPSQPLPSLPQDPPVDGTLQKKPDPFKIWAQSRSMYESRLPDYQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTGYGLAKVDGEMPPSPASSHHSSTQAPSLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSNNTVIPNAILPYDVEIQRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTPQQQAAPEPRNNTKPKQESFEFKAPQAPPPPAPTQSSNQDAEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRKVHLVLRRGDGSVPEYDGINDGNGLGAGAQTVSEVNSAPPESRPHPPSESSYPPDLHKSSRNDNKRTRESGQREHGHRSNHRHNNHRHRSPEKKSRSKGHGKDSGTSKSHRKKGDKKKSSTDDSHHHHHHHSNSRNQYHHHRQRHRSPERRRTRSAGDTLDSRHTQRNGRSPDRRHHRHSSPRRHSSPHRSPHRSGYRRSSPYRNRSPYRSPQRSRYQSPSRRPTDKEFDRGIDVTLKEPPPPKPPSRSESIPLLDRLSLEDTVMREPPSYRRDLSPMERAYRESTLLRGASRDGTFPRVRTPESDSAFRNYNSLLRGRSPVRVGNRHDAYRRDQSPVASSYRTRSLGRDLSPIRGFRDEEEEEVESQLSEYYSPLRSNYSRANNNVPEPRRNAYRESPKDLSI

Target ORF information:

RefSeq Version	XM_009305954.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane associated guanylate kinase, WW and PDZ domain containing 1b (magi1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305954.2

ORF Insert Sequence:

ATGGCTGGGC TGTTTACACT GAGACGGTGG AAGCGGGGAT TCGGTCAATA TTCACAAAAA 
TCCTCCTTTC GATTGAGTAC AACTCGCCCC CCTCGTGAAG GAGAGGTGCC GGGAGTAGAT 
TACAATTTCT TGTCAGTGGA CGAGTTTCTG AAGCTTGAAC AGAGTGGGAC ACTGTTGGAG 
ATCGGATCTT ACGAAGGTAA CTATTATGGC ACACCCAAGC CGCCTATTAA GCCCCCTAGT 
GGCAAAGTGA TCACTGGCGA TGCTCTTCAA GACAGCCTTC CCGGATCCCA GCCGTCCACC 
CCGCGTCGCA CCAAGTCCTA CAATGAGATG CAAAATGCTG GAATAGTGCC TACAGAGACT 
GAGGATGATG ACGAGGTTCC TGAAATGAAC AGCAGCTTCA CAGGAGACTC GAGCGAACCG 
GACGATCACC CCTCCGTGAG GCCCTTCGCC CGCGACCAAC TTCAACCCTA CGGGCTCAAC 
AACACCTCGG CGTCAACCAC GGAGAGCTCT CAGAACACAC ACACACACCC CGCCTACCCA 
ACGGAGGAGG ACCCACTGGG CCCTCTGCCT GAAAACTGGG AGATGGCGTA CACGGAGAAT 
GGAGAGGTCT ACTTTATAGA CCACAACACA AAGACAACAT CCTGGATCGA CCCTCGATGC 
CTGGACAAAC CTCAGAAACC CCTGGAGGAA TGTGAAGATG ATGAAGGGGT CCATACAGAG 
GAGCTGGACA ATGAACTAGA GCTGCCAACA GGCTGGGAGA AGATCGATGA TCCAGTGTAC 
GGGGTGTATT ATGTTGATCA TATTAACAGG AAGACGCAGT ATGAGAACCC AATTCTGGAA 
GCTAAAAGGA AGAAGCAACT GGAGCAGTCT CAGCCCGCTG AAGGTGAGCG GTTTATCCAA 
GAATGGATAG ACGAGCATTC GTCAGGAGCA CCGCTAGCGA GCTATGCTGC TAACCACCAA 
GAGACTTACA GAGACCCCGG CACCGGCCCT CCACTACCAA CTCAACTGGG GGCTAAACGA 
GGTAAGCCAT TTTTCACAAG GAACCCTGCA GAACTGAAGG GGACATTTAT TAACACCAAG 
CTCAAGAAGA GCAGGCGTGG GTTCGGCTTC ACTGTAGTAG GAGGAGATGA ACCCGATGAG 
TTCTTGCAGA TTAAGAGTCT TGTGCTCGAT GGACCCGCTG CCCTTGACGG AAAGATGGAA 
ACAGGTGATG TTATTGTAAG TGTGAACGAC ACTTGCGTTT TGGGTTACAC ACACGCCCAA 
GTTGTGAAGA TCTTCCAGTC AATCCCTATT GGCTCTATGG TCGACCTTGA ACTCTGCCGT 
GGCTACCCTT TGCCCTTTGA CCCTGATGAC CCCAACACCA GCCTGGTGAC TTCAGTGGCC 
ATTCTGGACA AGGAGCCAAT CATTGTCAAT GGAAAAGAAA GCTACGACTC ACCATCCAGC 
CACGGGAGCG AAACTACAGC TGGAGGCAGT AGCTTAAATG GCTCCAGTGA TCCTCCTCGC 
CCCCATAGCC CTTCAGCAGA GGTGGCTTCC AATGGCTCTC ATGGTTATCC CAGTGATGTG 
GTGACTTTGG CGTCCTCGAT CGCCACCCAG CCCGAGCTTA TTACTGTCCA CATGGAAAAA 
GGCGACAAGG GATTCGGCTT CACAATAGCA GACAGCCCGT CTGGAGGAGG CCAGCGAGTA 
AAGCAGATAG TGGATTACCC ACGATGCAGA GGCCTGAGGG AAGGTGACAT CATCATGGAG 
GTGAACAAGA GGAACGTGCA GAACCTGACG CACAATCAGG TGGTCGACCT GCTCAGCAAG 
TGTCCTAAAG GCAGTGAAGT GACCATGTTG GTGCAACGAG GGGTGGCACC TGCGAAGAAG 
AGCCCCAAAC TGCAACAGTT GGAGCGCAAG GACAGTCAGG GCAGCTCCCA GCACAGCGTG 
TCCAGTCAGC GCAGCATCCA CACAGACTCT CCTCTCCACG CCTCTCAGGC CCTGCTCAAC 
GAGTCGGCCG CCCCACCACC CCCGTCCCAG CCCTTGCCCA GCCTGCCCCA GGACCCTCCA 
GTAGACGGAA CCCTCCAAAA GAAGCCAGAT CCCTTTAAAA TCTGGGCCCA GTCCAGGAGC 
ATGTATGAAA GTAGGCTGCC TGATTACCAG GAGCAGGATA TCTTCCTGTG GAGGAAAGAT 
ACCGGCTTTG GATTCAGGAT CCTGGGAGGA AATGAACCTG GTGAGCCGAT TTATATTGGC 
CACATTGTGA AATATGGAGC TGCCGATGAA GATGGGCGTC TGCGATCAGG AGATGAGCTA 
ATTTGCGTGG ATGGAACGGC CGTTGTTGGA AAATCTCATC AGCTCGTCGT TCAGCTCATG 
CAACAAGCTG CAAAACAGGG TCACGTCAAC CTGACCGTGA GGCGCAAGAC TGGATACGGA 
CTGGCAAAGG TGGACGGTGA AATGCCTCCT TCTCCTGCCT CCTCTCACCA CAGCAGCACA 
CAGGCCCCAT CTCTGACCGA GGAGAAGCGC ACGCCACAGG GCAGCCAGAA CTCCCTAAAC 
ACGGTCAGCT CTGGGTCAGG CTCCACCAGC GGTATCGGTA GTGGAGGTGG CGGCGGCAGC 
AACAACACAG TCATTCCCAA TGCAATCCTG CCCTATGATG TGGAGATCCA GCGCGGAGAG 
AATGAGGGAT TTGGATTTGT CATTGTGTCG TCGGTGTCGA GGCCTGAGGC GGGAACAACT 
TTCGCTGGAA ACGCATGTGT GGCAATGCCT CATAAAATAG GTCGCATCAT CGAGGGAAGC 
CCAGCCGACC GCTGTGGAAA ACTGAAAGTC GGAGACCGGA TCCTGGCTGT GAACGGCTGC 
TCCATCACCA ACAAGTCCCA CTCGGATATC GTCAACCTGA TCAAAGAGGC TGGAAACACA 
GTGACGCTGC GGATCATTCC AGGAGACGAG TCCTCCAATG CATCACTGCT GACCAATGCT 
GAGAAGATTG CAACCATCAC TACCACACAC ACACCTCAAC AGCAAGCTGC ACCTGAGCCA 
AGAAACAACA CCAAACCAAA ACAGGAGTCT TTTGAGTTCA AAGCCCCGCA AGCGCCGCCT 
CCTCCAGCTC CAACACAATC TTCAAATCAG GATGCTGAGT TTTACTCTGT GGATCTGGAG 
AGGGACAATA AAGGCTTTGG TTTCAGCCTT AGAGGGGGTC GAGAGTACAA CATGGACCTG 
TATGTGCTGA GACTCGCTGA GGACGGAGCG GCTGTCAGGA ATGGGAAGAT GAGGGTTGGA 
GATGAGATCC TGGAGATCAA TGGAGAGAGC ACCAAGGGCA TGAAGCACGC CCGCGCCATT 
GAACTGATCA AAAACGGTGG CCGAAAAGTC CACCTGGTCC TAAGGAGGGG GGACGGCTCG 
GTGCCAGAAT ATGACGGCAT CAACGACGGC AACGGCCTCG GCGCCGGTGC ACAAACTGTT 
TCGGAAGTGA ACTCAGCTCC TCCCGAAAGT CGGCCCCACC CACCATCGGA GTCCAGCTAT 
CCACCAGACC TTCACAAATC ATCCCGAAAT GACAACAAAA GGACACGGGA GTCAGGCCAA 
CGGGAGCATG GTCACCGGTC AAACCACCGC CACAACAACC ACAGACACCG CTCCCCTGAA 
AAGAAGAGCA GGAGCAAAGG GCACGGTAAG GACAGTGGAA CTTCAAAGTC TCACAGGAAG 
AAGGGGGACA AGAAGAAATC TAGTACGGAT GATTCGCATC ATCATCACCA CCACCACAGC 
AACAGCCGCA ACCAGTACCA CCATCACCGC CAAAGGCACC GCTCCCCAGA GAGGCGCAGA 
ACTCGAAGTG CCGGGGACAC CTTAGACAGT AGACACACCC AGAGAAACGG ACGCTCACCA 
GACAGGCGGC ACCATCGCCA CTCTTCCCCA CGCAGACACA GCTCACCCCA TCGCTCACCT 
CACCGATCTG GTTATCGCCG AAGCTCTCCT TATCGCAACA GATCCCCCTA TCGCTCCCCT 
CAACGCTCCA GATACCAGTC TCCCTCAAGA CGTCCCACGG ATAAAGAGTT CGACAGGGGA 
ATTGACGTGA CCCTAAAGGA ACCTCCTCCT CCGAAGCCCC CATCCCGCTC TGAGAGCATT 
CCACTTTTAG ACCGGCTCAG TCTGGAAGAC ACTGTAATGA GAGAACCCCC CAGCTACCGT 
CGTGACCTTT CCCCAATGGA GCGCGCCTAT CGAGAGTCGA CCCTTCTCCG AGGGGCTTCG 
CGTGATGGCA CCTTTCCCCG TGTTCGAACG CCAGAGTCAG ATTCAGCTTT CCGGAATTAC 
AACTCATTAC TGAGGGGCCG TTCTCCAGTG AGAGTTGGGA ACAGACACGA TGCATATCGC 
AGAGATCAGT CTCCGGTTGC AAGCTCATAC CGAACAAGGA GTCTAGGTCG AGACCTGTCT 
CCCATTCGAG GGTTCCGCGA TGAGGAAGAA GAGGAGGTTG AGTCTCAGCT GAGCGAGTAT 
TATAGCCCGC TCCGATCTAA TTACAGCAGA GCTAACAACA ACGTACCTGA GCCCAGGAGG 
AATGCCTACA GAGAAAGCCC CAAAGATCTC AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	magi1b
Entrez Gene ID	474349
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 1b
Synonyms	MAGI-1,baiap1,fe48b06,magi1,wu:fe48b06,wu:fj98f08
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 1b Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 BAI1-associated protein 1 membrane associated guanylate kinase, WW and PDZ domain containing 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	MAGI1	XP_003309903.1
Danio rerio (zebrafish)	magi1b	NP_001007064.1
Mus musculus (house mouse)	Magi1	NP_001025021.1
Gallus gallus (chicken)	MAGI1	XP_003642069.1
Rattus norvegicus (Norway rat)	Magi1	NP_001025216.1
Homo sapiens (human)	MAGI1	NP_001028229.1
Canis lupus familiaris (dog)	MAGI1	XP_005633237.1
Bos taurus (cattle)	MAGI1	XP_002697009.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

magi1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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