nfil3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nfil3
Entrez Gene ID	445509
Full Name	nuclear factor, interleukin 3 regulated
Synonyms	E4BP4-1,zgc:100867
General protein information	Preferred Names nuclear factor, interleukin 3 regulated Names nuclear factor interleukin-3-regulated protein E4 binding protein 4-1 E4BP4 ze4bp4-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004120.2	NP_001004120.1	nuclear factor interleukin-3-regulated protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	NFIL3	NP_005375.2
Canis lupus familiaris (dog)	NFIL3	XP_003638841.1
Bos taurus (cattle)	NFIL3	NP_001068708.1
Gallus gallus (chicken)	NFIL3	NP_989949.1
Pan troglodytes (chimpanzee)	NFIL3	XP_003339220.1
Rattus norvegicus (Norway rat)	Nfil3	NP_446179.2
Mus musculus (house mouse)	Nfil3	NP_059069.1
	NFIL3	XP_001101854.1
Danio rerio (zebrafish)	nfil3	NP_001004120.1
Xenopus tropicalis (tropical clawed frog)	nfil3	NP_001015710.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
Ben-Moshe Livne Z, Alon S, Vallone D, Bayleyen Y, Tovin A, Shainer I, Nisembaum LG, Aviram I, Smadja-Storz S, Fuentes M, Falc?n J, Eisenberg E, Klein DC, Burgess HA, Foulkes NS, Gothilf Y
PLoS genetics12(11)e1006445(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Braasch I, Gehrke AR, Smith JJ, Kawasaki K, Manousaki T, Pasquier J, Amores A, Desvignes T, Batzel P, Catchen J, Berlin AM, Campbell MS, Barrell D, Martin KJ, Mulley JF, Ravi V, Lee AP, Nakamura T, Chalopin D, Fan S, Wcisel D, Ca?estro C, Sydes J, Beaudry FE, Sun Y, Hertel J, Beam MJ, Fasold M, Ishiyama M, Johnson J, Kehr S, Lara M, Letaw JH, Litman GW, Litman RT, Mikami M, Ota T, Saha NR, Williams L, Stadler PF, Wang H, Taylor JS, Fontenot Q, Ferrara A, Searle SM, Aken B, Yandell M, Schneider I, Yoder JA, Volff JN, Meyer A, Amemiya CT, Venkatesh B, Holland PW, Guiguen Y, Bobe J, Shubin NH, Di Palma F, Alf?ldi J, Lindblad-Toh K, Postlethwait JH
Nature genetics48(4)427-37(2016 Apr)

Progestins alter photo-transduction cascade and circadian rhythm network in eyes of zebrafish (Danio rerio).
Zhao Y, Fent K
Scientific reports621559(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that Nfil3 is enriched in the myeloid compartment of adult zebrafish including eosinophils.
Title: The role of Nfil3 in zebrafish hematopoiesis.

conclude that E4BP4/NIFL3 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.
Title: E4BP4 is a cardiac survival factor and essential for embryonic heart development.

The following nfil3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfil3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00120	NM_001004120.2 Latest version!	Danio rerio nuclear factor, interleukin 3 regulated (nfil3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nfil3 ( NM_001004120.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004120.1 Danio rerio nuclear factor, interleukin 3 regulated (nfil3), mRNA.

Price & Availability↑

CloneID	ODa00120
Clone ID Related Accession (Same CDS sequence)	NM_001004120.2
Accession Version	NM_001004120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Danio rerio(zebrafish)
Product	nuclear factor interleukin-3-regulated protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080210.1. On Aug 31, 2012 this sequence version replaced NM_001004120.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080210.1, EH446765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAAGCCA TTAAGAAAGA GCCCCCATGC AGTGGGCCCT ACGGTGGAGA GGACGCCTTG 
GTGCTGGCAG TAGCTTTGCA AGGGACAGAC AGGGACCTGA TTAACCACAA ACTGTCCACC 
CTACCTTTCA AGTCCAAGTC CACCAGCTGT CGCCGGAAAC GTGAGTTCAT ACCCGATGAG 
AAGAAAGACA ACCTATATTG GGAGAGAAGG CGCAAGAACA ATGAAGCAGC CAAGCGCTCC 
CGGGAAAAGC GCAGACTAAA TGACATGGTT CTGGAGAACA AGCTTATGGC CCTTGGGGAA 
GAGAACGCAT CGCTGAAAGC TGAGCTTTTG TCACTTAAAC TCAGGTTTGG TCTAGTGAGC 
TCAGCCGCTT ATGCTCAGGA GGTGCAGAAC ATCTCCACTT CCACTGCTGC GCTCTACCAG 
GACTTTATGT CACCCAGTGC CACCAAGGAT TCCTACCCAA GTGATCTGGA GCCAACGCGT 
TTGACCAGCA GCTGCATTTC GGTCATCAAG CACTCACCTC ACAGCGCCTT GTCCGACGGA 
TCTGACTCTA GCACAGTTAC CCAGGGAAGC CCCCTAATAA ATATTTCTAG ATCCCCTGAC 
AGCATCAAAC AAGAACCCCT GGAAACTGGC AGATACTCCA AAGAGAGGAT CAGTCCTTAT 
GAACTCTACA GGAACTACCT TAGTAGTCCT TTTCCTGGGA ACTTCTCCCA GCCATCTCCG 
TTCTTACAAA TCGCTAGATC GTCTAGCAAT TCGCCACGAA CATCTGATGG CGACGATGGC 
GCCGTAAGCA AGTCCTCCGA TAATGAGGAT GAACAGCAGG TCCCAAAGGG TCCAGTTCCA 
ACCAGGTCTG ATTCACAGAG CGTGATTGTG TCAACTCTCA AAGTGCCAGA TTCCAGTGCC 
TCAGCTTTGC CTCACAAGTT GAGGATCAAA GCCCGGGCCA TCCAGATTAA AGTGGAGGCA 
ATCGATCCTG ATTATGAATC ATCTGGAAAG TCCTCCTTTC CCGTTGACAT GTCTGCAAGA 
AGACGCTACC AAATGAGTCA GTGTGCTACA CCCGAGTACA TTCAATCATC TCTTAGCCCG 
ATGTCCTTCC AGATGACCAA TGTTCAGGAC TGGAATCACC GACCGAAGGA ATGGCACGAG 
GATCACCAGG AGGCCCTAAC TAGCTACAAA TACCGGCAAT GTCCAGACTC ACCAAGACCT 
GTGCCTAACA AACTCATCGT TGACCTTGAA AATGACTCGT ATGCCAACTC TGAATCTGAG 
AATTTGTACT TGAAGCAGGG CATTGAGGAT TTGTCGGCAG AAGTGGCCTG CTTGAAAAGA 
CTGATTTCAA AACAGCAGGG GTCTGTTATT GAGTCCACGA AAAGCACTAC TGAAATTGAC 
TCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004120.1
CDS	283..1671
Translation	MQAIKKEPPCSGPYGGEDALVLAVALQGTDRDLINHKLSTLPFKSKSTSCRRKREFIPDEKKDNLYWERRRKNNEAAKRSREKRRLNDMVLENKLMALGEENASLKAELLSLKLRFGLVSSAAYAQEVQNISTSTAALYQDFMSPSATKDSYPSDLEPTRLTSSCISVIKHSPHSALSDGSDSSTVTQGSPLINISRSPDSIKQEPLETGRYSKERISPYELYRNYLSSPFPGNFSQPSPFLQIARSSSNSPRTSDGDDGAVSKSSDNEDEQQVPKGPVPTRSDSQSVIVSTLKVPDSSASALPHKLRIKARAIQIKVEAIDPDYESSGKSSFPVDMSARRRYQMSQCATPEYIQSSLSPMSFQMTNVQDWNHRPKEWHEDHQEALTSYKYRQCPDSPRPVPNKLIVDLENDSYANSESENLYLKQGIEDLSAEVACLKRLISKQQGSVIESTKSTTEIDSS

Target ORF information:

RefSeq Version	NM_001004120.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor, interleukin 3 regulated (nfil3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004120.2

ORF Insert Sequence:

ATGCAAGCCA TTAAGAAAGA GCCCCCATGC AGTGGGCCCT ACGGTGGAGA GGACGCCTTG 
GTGCTGGCAG TAGCTTTGCA AGGGACAGAC AGGGACCTGA TTAACCACAA ACTGTCCACC 
CTACCTTTCA AGTCCAAGTC CACCAGCTGT CGCCGGAAAC GTGAGTTCAT ACCCGATGAG 
AAGAAAGACA ACCTATATTG GGAGAGAAGG CGCAAGAACA ATGAAGCAGC CAAGCGCTCC 
CGGGAAAAGC GCAGACTAAA TGACATGGTT CTGGAGAACA AGCTTATGGC CCTTGGGGAA 
GAGAACGCAT CGCTGAAAGC TGAGCTTTTG TCACTTAAAC TCAGGTTTGG TCTAGTGAGC 
TCAGCCGCTT ATGCTCAGGA GGTGCAGAAC ATCTCCACTT CCACTGCTGC GCTCTACCAG 
GACTTTATGT CACCCAGTGC CACCAAGGAT TCCTACCCAA GTGATCTGGA GCCAACGCGT 
TTGACCAGCA GCTGCATTTC GGTCATCAAG CACTCACCTC ACAGCGCCTT GTCCGACGGA 
TCTGACTCTA GCACAGTTAC CCAGGGAAGC CCCCTAATAA ATATTTCTAG ATCCCCTGAC 
AGCATCAAAC AAGAACCCCT GGAAACTGGC AGATACTCCA AAGAGAGGAT CAGTCCTTAT 
GAACTCTACA GGAACTACCT TAGTAGTCCT TTTCCTGGGA ACTTCTCCCA GCCATCTCCG 
TTCTTACAAA TCGCTAGATC GTCTAGCAAT TCGCCACGAA CATCTGATGG CGACGATGGC 
GCCGTAAGCA AGTCCTCCGA TAATGAGGAT GAACAGCAGG TCCCAAAGGG TCCAGTTCCA 
ACCAGGTCTG ATTCACAGAG CGTGATTGTG TCAACTCTCA AAGTGCCAGA TTCCAGTGCC 
TCAGCTTTGC CTCACAAGTT GAGGATCAAA GCCCGGGCCA TCCAGATTAA AGTGGAGGCA 
ATCGATCCTG ATTATGAATC ATCTGGAAAG TCCTCCTTTC CCGTTGACAT GTCTGCAAGA 
AGACGCTACC AAATGAGTCA GTGTGCTACA CCCGAGTACA TTCAATCATC TCTTAGCCCG 
ATGTCCTTCC AGATGACCAA TGTTCAGGAC TGGAATCACC GACCGAAGGA ATGGCACGAG 
GATCACCAGG AGGCCCTAAC TAGCTACAAA TACCGGCAAT GTCCAGACTC ACCAAGACCT 
GTGCCTAACA AACTCATCGT TGACCTTGAA AATGACTCGT ATGCCAACTC TGAATCTGAG 
AATTTGTACT TGAAGCAGGG CATTGAGGAT TTGTCGGCAG AAGTGGCCTG CTTGAAAAGA 
CTGATTTCAA AACAGCAGGG GTCTGTTATT GAGTCCACGA AAAGCACTAC TGAAATTGAC 
TCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
PLoS genetics12(11)e1006445(2016 Nov)
Ben-Moshe Livne Z,Alon S,Vallone D,Bayleyen Y,Tovin A,Shainer I,Nisembaum LG,Aviram I,Smadja-Storz S,Fuentes M,Falc?n J,Eisenberg E,Klein DC,Burgess HA,Foulkes NS,Gothilf Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Nature genetics48(4)427-37(2016 Apr)
Braasch I,Gehrke AR,Smith JJ,Kawasaki K,Manousaki T,Pasquier J,Amores A,Desvignes T,Batzel P,Catchen J,Berlin AM,Campbell MS,Barrell D,Martin KJ,Mulley JF,Ravi V,Lee AP,Nakamura T,Chalopin D,Fan S,Wcisel D,Ca?estro C,Sydes J,Beaudry FE,Sun Y,Hertel J,Beam MJ,Fasold M,Ishiyama M,Johnson J,Kehr S,Lara M,Letaw JH,Litman GW,Litman RT,Mikami M,Ota T,Saha NR,Williams L,Stadler PF,Wang H,Taylor JS,Fontenot Q,Ferrara A,Searle SM,Aken B,Yandell M,Schneider I,Yoder JA,Volff JN,Meyer A,Amemiya CT,Venkatesh B,Holland PW,Guiguen Y,Bobe J,Shubin NH,Di Palma F,Alf?ldi J,Lindblad-Toh K,Postlethwait JH

Progestins alter photo-transduction cascade and circadian rhythm network in eyes of zebrafish (Danio rerio).
Scientific reports621559(2016 Feb)
Zhao Y,Fent K