tnfsf10 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnfsf10
Entrez Gene ID	436866
Full Name	TNF superfamily member 10
Synonyms	tnfsf10l2,zgc:92320
General protein information	Preferred Names TNF superfamily member 10 Names tumor necrosis factor ligand superfamily member 10 DL2 TNFSF10-1 tumor necrosis factor (ligand) superfamily, member 10 like 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002593.2	NP_001002593.1	tumor necrosis factor ligand superfamily member 10 isoform 1
NM_001328527.1	NP_001315456.1	tumor necrosis factor ligand superfamily member 10 isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TNFSF10	XP_002684963.1
Mus musculus (house mouse)	Tnfsf10	NP_033451.1
Homo sapiens (human)	TNFSF10	NP_003801.1
Pan troglodytes (chimpanzee)	TNFSF10	NP_001233349.1
Canis lupus familiaris (dog)	TNFSF10	NP_001124308.1
Gallus gallus (chicken)	TNFSF10	NP_989710.1
Danio rerio (zebrafish)	tnfsf10	NP_001002593.1
Xenopus tropicalis (tropical clawed frog)	tnfsf10	XP_002931598.2
	TNFSF10	XP_001084768.1
Rattus norvegicus (Norway rat)	Tnfsf10	NP_663714.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish Model for Functional Screening of Flow-Responsive Genes.
Serbanovic-Canic J, de Luca A, Warboys C, Ferreira PF, Luong LA, Hsiao S, Gauci I, Mahmoud M, Feng S, Souilhol C, Bowden N, Ashton JP, Walczak H, Firmin D, Krams R, Mason JC, Haskard DO, Sherwin S, Ridger V, Chico TJ, Evans PC
Arteriosclerosis, thrombosis, and vascular biology37(1)130-143(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genome.
Yi Y, Qi H, Yuan J, Wang R, Weng S, He J, Dong C
Fish & shellfish immunology45(2)757-70(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnfsf10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnfsf10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98515	NM_001002593.2 Latest version!	Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
ODa98516	NM_001328527.1 Latest version!	Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnfsf10 ( NM_001002593.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002593.1 Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa98515
Clone ID Related Accession (Same CDS sequence)	NM_001002593.2
Accession Version	NM_001002593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-22
Organism	Danio rerio(zebrafish)
Product	tumor necrosis factor ligand superfamily member 10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR376848.7, BC076005.1, EH499289.1, EH448113.1 and EH525548.1. On Jun 17, 2016 this sequence version replaced NM_001002593.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01009145.1, GFIL01041429.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTCAGCA TGACAAGCTC ACACACCATG CAGTATATCG GACTTCTGCT GCTGGCTGCA 
ATCCTCCTGC AAACAATAGC CGTGGCTGTA ACTTTCATTT ACTTCAGCAA CGTCTTGTCG 
ACGATGAAAG AGACGTTCTC CAAGAGCAGC GTGTCTTGTC TTATGCGGGC CAACCTCAGG 
ACTATTAAGG GTCAGGAGCT GAACGGGGCG GAGGGCAAGG ACGACCCCTG CTGGCAGGTC 
ACGCAGCAGC TCCACTTTCT GATTGAAAAG TCAATGTCCA GTCGATATCA GAAAGAAATT 
ACATCTGCTG TCAAAGATGA AGTCTCTCGT GTTCTTCCCT CACTGGTGAT CCAAGATCAA 
GAGGATTCCT CTCGGCCTAA GATTGCAGCC CATGTGACTG GGAGTTATAC ACCAGAATCA 
GAAAAAGACG GAGCAGGTCT ACCAAACAGG AAGGTCTACG GACAGAAGAT CCAGTCTTGG 
GAGTCCGAAA AAGGTCTGGC TTTTCTCCAG AACGTGGAGT TAAGTGACGG AGAATTGGTG 
GTACCTCAGG CTGGCCTTTA TTATATCTAC TCGCAAACGT ATTTTCGTCA TACGCTGATC 
GAGGAAGACG AAAGTGCTCG CGAGGATGAG TACGGCTCGA TGGGCGAATC GGTTCGAGGG 
AAACCGATGT TGCAGTATGT TTATAAAAAA GTAAGCTCCT ATCAGGTGCC GATTCTGCTA 
ATGAAGAATG CTCGGACGAC ATGTTGGTCA CGTGACTCCG AATACGGCCT TTATTCGATC 
TACCAAGCTG GGCTTTTTCA ACTCGGAAGT GGAGATCGAG TGTTTGTGAC AGTCAGCAAC 
GTAAGCACAA TTGACATGGA TGAGAAGTCA AGCTTCTTCG GAGCATTTCT GGTGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002593.1
CDS	64..963
Translation	MVSMTSSHTMQYIGLLLLAAILLQTIAVAVTFIYFSNVLSTMKETFSKSSVSCLMRANLRTIKGQELNGAEGKDDPCWQVTQQLHFLIEKSMSSRYQKEITSAVKDEVSRVLPSLVIQDQEDSSRPKIAAHVTGSYTPESEKDGAGLPNRKVYGQKIQSWESEKGLAFLQNVELSDGELVVPQAGLYYIYSQTYFRHTLIEEDESAREDEYGSMGESVRGKPMLQYVYKKVSSYQVPILLMKNARTTCWSRDSEYGLYSIYQAGLFQLGSGDRVFVTVSNVSTIDMDEKSSFFGAFLVS

Target ORF information:

RefSeq Version	NM_001002593.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002593.2

ORF Insert Sequence:

ATGGTCAGCA TGACAAGCTC ACACACCATG CAGTATATCG GACTTCTGCT GCTGGCTGCA 
ATCCTCCTGC AAACAATAGC CGTGGCTGTA ACTTTCATTT ACTTCAGCAA CGTCTTGTCG 
ACGATGAAAG AGACGTTCTC CAAGAGCAGC GTGTCTTGTC TTATGCGGGC CAACCTCAGG 
ACTATTAAGG GTCAGGAGCT GAACGGGGCG GAGGGCAAGG ACGACCCCTG CTGGCAGGTC 
ACGCAGCAGC TCCACTTTCT GATTGAAAAG TCAATGTCCA GTCGATATCA GAAAGAAATT 
ACATCTGCTG TCAAAGATGA AGTCTCTCGT GTTCTTCCCT CACTGGTGAT CCAAGATCAA 
GAGGATTCCT CTCGGCCTAA GATTGCAGCC CATGTGACTG GGAGTTATAC ACCAGAATCA 
GAAAAAGACG GAGCAGGTCT ACCAAACAGG AAGGTCTACG GACAGAAGAT CCAGTCTTGG 
GAGTCCGAAA AAGGTCTGGC TTTTCTCCAG AACGTGGAGT TAAGTGACGG AGAATTGGTG 
GTACCTCAGG CTGGCCTTTA TTATATCTAC TCGCAAACGT ATTTTCGTCA TACGCTGATC 
GAGGAAGACG AAAGTGCTCG CGAGGATGAG TACGGCTCGA TGGGCGAATC GGTTCGAGGG 
AAACCGATGT TGCAGTATGT TTATAAAAAA GTAAGCTCCT ATCAGGTGCC GATTCTGCTA 
ATGAAGAATG CTCGGACGAC ATGTTGGTCA CGTGACTCCG AATACGGCCT TTATTCGATC 
TACCAAGCTG GGCTTTTTCA ACTCGGAAGT GGAGATCGAG TGTTTGTGAC AGTCAGCAAC 
GTAAGCACAA TTGACATGGA TGAGAAGTCA AGCTTCTTCG GAGCATTTCT GGTGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnfsf10 ( NM_001328527.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315456.1 Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa98516
Clone ID Related Accession (Same CDS sequence)	NM_001328527.1
Accession Version	NM_001328527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-22
Organism	Danio rerio(zebrafish)
Product	tumor necrosis factor ligand superfamily member 10 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR376848.7, EH499289.1, EH448113.1, BC076005.1 and EH525548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EH499289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTCAGCA TGACAAGCTC ACACACCATG CAGTATATCG GACTTCTGCT GCTGGCTGCA 
ATCCTCCTGC AAACAATAGC CGTGGCTGTA ACTTTCATTT ACTTCAGCAA CGTCTTGTCG 
ACGATGAAAG AGACGTTCTC CAAGAGCAGC GTGTCTTGTC TTATGCGGGC CAACCTCAGG 
ACTATTAAGG GTCAGGAGCT GAACGGGGCG GAGGGCAAGG ACGACCCCTG CTGGCAGGTC 
ACGCAGCAGC TCCACTTTCT GATTGAAAAG TCAATGTCCA GTCGATATCA GAAAGAAATT 
ACATCTGCTG TCAAAGATCA AGAGGATTCC TCTCGGCCTA AGATTGCAGC CCATGTGACT 
GGGAGTTATA CACCAGAATC AGAAAAAGAC GGAGCAGGTC TACCAAACAG GAAGGTCTAC 
GGACAGAAGA TCCAGTCTTG GGAGTCCGAA AAAGGTCTGG CTTTTCTCCA GAACGTGGAG 
TTAAGTGACG GAGAATTGGT GGTACCTCAG GCTGGCCTTT ATTATATCTA CTCGCAAACG 
TATTTTCGTC ATACGCTGAT CGAGGAAGAC GAAAGTGCTC GCGAGGATGA GTACGGCTCG 
ATGGGCGAAT CGGTTCGAGG GAAACCGATG TTGCAGTATG TTTATAAAAA AGTAAGCTCC 
TATCAGGTGC CGATTCTGCT AATGAAGAAT GCTCGGACGA CATGTTGGTC ACGTGACTCC 
GAATACGGCC TTTATTCGAT CTACCAAGCT GGGCTTTTTC AACTCGGAAG TGGAGATCGA 
GTGTTTGTGA CAGTCAGCAA CGTAAGCACA ATTGACATGG ATGAGAAGTC AAGCTTCTTC 
GGAGCATTTC TGGTGAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315456.1
CDS	64..924
Translation	MVSMTSSHTMQYIGLLLLAAILLQTIAVAVTFIYFSNVLSTMKETFSKSSVSCLMRANLRTIKGQELNGAEGKDDPCWQVTQQLHFLIEKSMSSRYQKEITSAVKDQEDSSRPKIAAHVTGSYTPESEKDGAGLPNRKVYGQKIQSWESEKGLAFLQNVELSDGELVVPQAGLYYIYSQTYFRHTLIEEDESAREDEYGSMGESVRGKPMLQYVYKKVSSYQVPILLMKNARTTCWSRDSEYGLYSIYQAGLFQLGSGDRVFVTVSNVSTIDMDEKSSFFGAFLVS

Target ORF information:

RefSeq Version	NM_001328527.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TNF superfamily member 10 (tnfsf10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328527.1

ORF Insert Sequence:

ATGGTCAGCA TGACAAGCTC ACACACCATG CAGTATATCG GACTTCTGCT GCTGGCTGCA 
ATCCTCCTGC AAACAATAGC CGTGGCTGTA ACTTTCATTT ACTTCAGCAA CGTCTTGTCG 
ACGATGAAAG AGACGTTCTC CAAGAGCAGC GTGTCTTGTC TTATGCGGGC CAACCTCAGG 
ACTATTAAGG GTCAGGAGCT GAACGGGGCG GAGGGCAAGG ACGACCCCTG CTGGCAGGTC 
ACGCAGCAGC TCCACTTTCT GATTGAAAAG TCAATGTCCA GTCGATATCA GAAAGAAATT 
ACATCTGCTG TCAAAGATCA AGAGGATTCC TCTCGGCCTA AGATTGCAGC CCATGTGACT 
GGGAGTTATA CACCAGAATC AGAAAAAGAC GGAGCAGGTC TACCAAACAG GAAGGTCTAC 
GGACAGAAGA TCCAGTCTTG GGAGTCCGAA AAAGGTCTGG CTTTTCTCCA GAACGTGGAG 
TTAAGTGACG GAGAATTGGT GGTACCTCAG GCTGGCCTTT ATTATATCTA CTCGCAAACG 
TATTTTCGTC ATACGCTGAT CGAGGAAGAC GAAAGTGCTC GCGAGGATGA GTACGGCTCG 
ATGGGCGAAT CGGTTCGAGG GAAACCGATG TTGCAGTATG TTTATAAAAA AGTAAGCTCC 
TATCAGGTGC CGATTCTGCT AATGAAGAAT GCTCGGACGA CATGTTGGTC ACGTGACTCC 
GAATACGGCC TTTATTCGAT CTACCAAGCT GGGCTTTTTC AACTCGGAAG TGGAGATCGA 
GTGTTTGTGA CAGTCAGCAA CGTAAGCACA ATTGACATGG ATGAGAAGTC AAGCTTCTTC 
GGAGCATTTC TGGTGAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish Model for Functional Screening of Flow-Responsive Genes.
Arteriosclerosis, thrombosis, and vascular biology37(1)130-143(2017 Jan)
Serbanovic-Canic J,de Luca A,Warboys C,Ferreira PF,Luong LA,Hsiao S,Gauci I,Mahmoud M,Feng S,Souilhol C,Bowden N,Ashton JP,Walczak H,Firmin D,Krams R,Mason JC,Haskard DO,Sherwin S,Ridger V,Chico TJ,Evans PC

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genome.
Fish & shellfish immunology45(2)757-70(2015 Aug)
Yi Y,Qi H,Yuan J,Wang R,Weng S,He J,Dong C