aqp12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	aqp12
Entrez Gene ID	436844
Full Name	aquaporin 12
Synonyms	wu:fb60e09,zgc:152755,zgc:92747
General protein information	Preferred Names aquaporin 12 Names aquaporin-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045862.2	NP_001039327.1	aquaporin-like

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AQP12B	XP_005205120.1
Rattus norvegicus (Norway rat)	Aqp12a	NP_001102479.1
Gallus gallus (chicken)	AQP12	NP_001103149.1
Mus musculus (house mouse)	Aqp12	NP_808255.1
Xenopus tropicalis (tropical clawed frog)	LOC100485225	XP_002935146.2
Homo sapiens (human)	AQP12A	NP_945349.1
Danio rerio (zebrafish)	aqp12	NP_001039327.1
Homo sapiens (human)	AQP12B	NP_001095937.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Genome Wide Identification, Phylogeny, and Expression of Aquaporin Genes in Common Carp (Cyprinus carpio).
Dong C, Chen L, Feng J, Xu J, Mahboob S, Al-Ghanim K, Li X, Xu P
PloS one11(12)e0166160(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Aquaporin 1 Is Involved in Acid Secretion by Ionocytes of Zebrafish Embryos through Facilitating CO2 Transport.
Horng JL, Chao PL, Chen PY, Shih TH, Lin LY
PloS one10(8)e0136440(2015)

The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals.
Tingaud-Sequeira A, Calusinska M, Finn RN, Chauvign? F, Lozano J, Cerd? J
BMC evolutionary biology1038(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following aqp12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aqp12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35342	NM_001045862.2 Latest version!	Danio rerio aquaporin 12 (aqp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aqp12 ( NM_001045862.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001039327.1 Danio rerio aquaporin 12 (aqp12), mRNA.

Price & Availability↑

CloneID	ODa35342
Clone ID Related Accession (Same CDS sequence)	NM_001045862.2
Accession Version	NM_001045862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	aquaporin-like
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324210.12, BC121752.1 and BC076225.1. On or before May 28, 2009 this sequence version replaced NM_001002571.1, NM_001045862.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01001512.1, GFIL01001351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505375, SAMEA3505378 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCC TGAACTCTTC TCTGGGCTTC TTCCTGGCGG TGGTGGGTTT GAGTGTGTCG 
GGCCGTCTTC TGCTGCGCCG ATGGACCGTC CTGCTGGAGT TGGTCTCCGC GTTCGCTCTG 
TGCGCCTGCA GACTGGAGGT GGACACCATC GCAGAGGTGG GACAGTGGGC CGGAGCACTC 
GGGCCAGATG TTGCGGTCAC AATGCTGTTC CTGTCCATCG CCGTACACAC TGCCGTCATG 
CAGGACGTGA GTGGAAACCC GGCGGTGACG CTGCTGCGGC TCCTGCAGAG GGATGTCAGC 
GTAGTGGTGG CAGTTCTGAG CATAGCGGCG CAGCTGATTG GAGCGTTTCT GGCTCTGGAA 
GTGGCCGGCA GGTTCTGGGC GATGGAGCTG AGCGATATGC ACATGATCAA GAACCTGATG 
ATGTCCGAGT GCAGCACGTC TCTGAGGGTC TCCACAGCTC TCGGGGTCTC CACTGAAGCT 
CTCGCTGCGC TGCTGCTGCT CCTGCTGCAC CTCGTCCTGA AGAACAGATC ACAAATGCTG 
AAGGTCCCTG CGCTCTCTGT GGCGCTCACG CTCATCGCTT ACACCGCAAA CAACTACACA 
TCTGGATATG TGAATCCGGC CCTGGCCTAT GCAGTGACCT TCACCTGCCC TGGACACTCG 
TTCCTGGTGT ATTCACTGGT CTACTGGCTC GGACCACTCA TTGGTGTGTT TCTTGCAGTC 
TTCCTGTATT TGGGAAATAT CCCACTGCTG TTCAGCAAGA ATCTGCTCTA CTCCAAGAAA 
AATCGATTCC GGCTGCCGAA AGGAAAAACC AATGATGAGA AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039327.1
CDS	78..908
Translation	MSGLNSSLGFFLAVVGLSVSGRLLLRRWTVLLELVSAFALCACRLEVDTIAEVGQWAGALGPDVAVTMLFLSIAVHTAVMQDVSGNPAVTLLRLLQRDVSVVVAVLSIAAQLIGAFLALEVAGRFWAMELSDMHMIKNLMMSECSTSLRVSTALGVSTEALAALLLLLLHLVLKNRSQMLKVPALSVALTLIAYTANNYTSGYVNPALAYAVTFTCPGHSFLVYSLVYWLGPLIGVFLAVFLYLGNIPLLFSKNLLYSKKNRFRLPKGKTNDEKSS

Target ORF information:

RefSeq Version	NM_001045862.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio aquaporin 12 (aqp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045862.2

ORF Insert Sequence:

ATGTCGGGCC TGAACTCTTC TCTGGGCTTC TTCCTGGCGG TGGTGGGTTT GAGTGTGTCG 
GGCCGTCTTC TGCTGCGCCG ATGGACCGTC CTGCTGGAGT TGGTCTCCGC GTTCGCTCTG 
TGCGCCTGCA GACTGGAGGT GGACACCATC GCAGAGGTGG GACAGTGGGC CGGAGCACTC 
GGGCCAGATG TTGCGGTCAC AATGCTGTTC CTGTCCATCG CCGTACACAC TGCCGTCATG 
CAGGACGTGA GTGGAAACCC GGCGGTGACG CTGCTGCGGC TCCTGCAGAG GGATGTCAGC 
GTAGTGGTGG CAGTTCTGAG CATAGCGGCG CAGCTGATTG GAGCGTTTCT GGCTCTGGAA 
GTGGCCGGCA GGTTCTGGGC GATGGAGCTG AGCGATATGC ACATGATCAA GAACCTGATG 
ATGTCCGAGT GCAGCACGTC TCTGAGGGTC TCCACAGCTC TCGGGGTCTC CACTGAAGCT 
CTCGCTGCGC TGCTGCTGCT CCTGCTGCAC CTCGTCCTGA AGAACAGATC ACAAATGCTG 
AAGGTCCCTG CGCTCTCTGT GGCGCTCACG CTCATCGCTT ACACCGCAAA CAACTACACA 
TCTGGATATG TGAATCCGGC CCTGGCCTAT GCAGTGACCT TCACCTGCCC TGGACACTCG 
TTCCTGGTGT ATTCACTGGT CTACTGGCTC GGACCACTCA TTGGTGTGTT TCTTGCAGTC 
TTCCTGTATT TGGGAAATAT CCCACTGCTG TTCAGCAAGA ATCTGCTCTA CTCCAAGAAA 
AATCGATTCC GGCTGCCGAA AGGAAAAACC AATGATGAGA AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Genome Wide Identification, Phylogeny, and Expression of Aquaporin Genes in Common Carp (Cyprinus carpio).
PloS one11(12)e0166160(2016)
Dong C,Chen L,Feng J,Xu J,Mahboob S,Al-Ghanim K,Li X,Xu P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Aquaporin 1 Is Involved in Acid Secretion by Ionocytes of Zebrafish Embryos through Facilitating CO2 Transport.
PloS one10(8)e0136440(2015)
Horng JL,Chao PL,Chen PY,Shih TH,Lin LY

The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals.
BMC evolutionary biology1038(2010 Feb)
Tingaud-Sequeira A,Calusinska M,Finn RN,Chauvign? F,Lozano J,Cerd? J