rfng cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rfng
Entrez Gene ID	414848
Full Name	RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms	fd16f05,rfg,wu:fd16f05,zgc:136694
General protein information	Preferred Names RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase Names beta-1,3-N-acetylglucosaminyltransferase radical fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase radical fringe homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001830.2	NP_001001830.2	beta-1,3-N-acetylglucosaminyltransferase radical fringe precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RFNG	XP_540495.4
Bos taurus (cattle)	RFNG	XP_002696180.2
Drosophila melanogaster (fruit fly)	fng	NP_524191.1
Mus musculus (house mouse)	Rfng	NP_033079.1
Rattus norvegicus (Norway rat)	Rfng	NP_068621.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006439	XP_316475.4
Xenopus tropicalis (tropical clawed frog)	rfng	XP_002937356.2
Danio rerio (zebrafish)	rfng	NP_001038801.1
Homo sapiens (human)	RFNG	NP_002908.1
	LOC719330	XP_002800683.1
Gallus gallus (chicken)	RFNG	NP_990278.1

Gene Ontology
Bibliography

Related articles in PubMed

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development.
Thomas-Jinu S, Gordon PM, Fielding T, Taylor R, Smith BN, Snowden V, Blanc E, Vance C, Topp S, Wong CH, Bielen H, Williams KL, McCann EP, Nicholson GA, Pan-Vazquez A, Fox AH, Bond CS, Talbot WS, Blair IP, Shaw CE, Houart C
Neuron94(2)322-336.e5(2017 Apr)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition.
Chang J, Skromne I, Ho RK
Developmental biology410(2)178-189(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The sequence and embryonic expression of rfng were studied.
Title: Sequence and embryonic expression of three zebrafish fringe genes: lunatic fringe, radical fringe, and manic fringe.

The following rfng gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rfng cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53254	NM_001001830.2 Latest version!	Danio rerio RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (rfng), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rfng ( NM_001001830.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001001830.2 Danio rerio RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (rfng), mRNA.

Price & Availability↑

CloneID	ODa53254
Clone ID Related Accession (Same CDS sequence)	NM_001001830.2
Accession Version	NM_001001830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	beta-1,3-N-acetylglucosaminyltransferase radical fringe precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548040.19. On or before Feb 4, 2016 this sequence version replaced NM_001045336.1, NM_001001830.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF510993.1, BC118683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476783 [ECO:0000348] ##Evidence-Data-END##

ATGCACTTAT CCCATGTCGG CTCCAACAAA ACCTGCTTTC TGCTTTCCCT CGCATTCTGT 
GCCCTGCTGG TGCTGCTTAT TCCCTCCTTA CAACCTCCAC AGCGTCAGGC GGATCTGCCT 
CAGCCGCGGC CCCATGCCAA ACCCGCCAAA CACCCACTCA AAAACTCTCT CTACAGACCA 
AATGAAGACA CCAGGGGTGG TAGTTTTACT CAAACAACTC CACCTGAGAA CCTGCAGAAG 
ATGGACGATC TAAACTCGCA CTACAGAGAC TTCCTGGACT TAAGAGACAT TTTTATTGCT 
GTGAAGACCA CTAGGAAGTA TCACAAGTCC AGATTGCAGC TCCTGTCCCA GACCTGGGTG 
TCCAGAGCGA AGGAGCAGAC GTTTATCTTC ACTGATGGGG AGGACAAGGA GCTCCGGCTG 
AAAGCAGGGC TGAACATCAT CAACACCAAC TGCTCGGCGG CTCACACACG CCAAGCCCTG 
TGCTGCAAGA TGTCTGTGGA GTATGACAAG TTCATCGAGT CACAGAAAAA GTGGTTTTGT 
CATGTGGATG ATGATAACTA TGTGATCCTG CCCAGTCTAC TGGAGCTTCT GTCCTCGTAC 
TCTCACACAC AGGATGTGTA TCTGGGCCGG CCTAGCCTGG ATCATCCTAT AGAGGCTGCA 
GAGAGAGTCA AGAGCGACGG ATCGGTGTCA GTGAAGTTCT GGTTTGCCAC TGGTGGAGCT 
GGTTTCTGCA TCAGTCGAGG TTTAGCGCTC AAAATGAGTC CATGGGCCAG TCTTGGAAAC 
TTTATCACAA CAGCAGAGAA GATCCGTCTG CCAGATGACT GTACAATTGG CTACATCATC 
GAGGCGCTGC TGGAGGTTCC TCTCACCCAC ACAGGCCTGT TTCACTCACA CCTGGAGAAC 
CTGCAGAGAC TGCCTGCAGA AAACATCCTG CGACAGGTGA CCCTCAGTTA TGGTGGATTC 
GAGAACAGGA GGAATGTGGT CAGTGTTGGA GGAGCGTTTT CATTGGCTGA AGACCCAACA 
CGGTTTAAAA CGGTTCACTG CAAGCTCTAC CCAGACACCG AGTGGTGCCC ACGCAAGACT 
CGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001830.2
CDS	499..1587
Translation	MHLSHVGSNKTCFLLSLAFCALLVLLIPSLQPPQRQADLPQPRPHAKPAKHPLKNSLYRPNEDTRGGSFTQTTPPENLQKMDDLNSHYRDFLDLRDIFIAVKTTRKYHKSRLQLLSQTWVSRAKEQTFIFTDGEDKELRLKAGLNIINTNCSAAHTRQALCCKMSVEYDKFIESQKKWFCHVDDDNYVILPSLLELLSSYSHTQDVYLGRPSLDHPIEAAERVKSDGSVSVKFWFATGGAGFCISRGLALKMSPWASLGNFITTAEKIRLPDDCTIGYIIEALLEVPLTHTGLFHSHLENLQRLPAENILRQVTLSYGGFENRRNVVSVGGAFSLAEDPTRFKTVHCKLYPDTEWCPRKTRH

Target ORF information:

RefSeq Version	NM_001001830.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (rfng), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001830.2

ORF Insert Sequence:

ATGCACTTAT CCCATGTCGG CTCCAACAAA ACCTGCTTTC TGCTTTCCCT CGCATTCTGT 
GCCCTGCTGG TGCTGCTTAT TCCCTCCTTA CAACCTCCAC AGCGTCAGGC GGATCTGCCT 
CAGCCGCGGC CCCATGCCAA ACCCGCCAAA CACCCACTCA AAAACTCTCT CTACAGACCA 
AATGAAGACA CCAGGGGTGG TAGTTTTACT CAAACAACTC CACCTGAGAA CCTGCAGAAG 
ATGGACGATC TAAACTCGCA CTACAGAGAC TTCCTGGACT TAAGAGACAT TTTTATTGCT 
GTGAAGACCA CTAGGAAGTA TCACAAGTCC AGATTGCAGC TCCTGTCCCA GACCTGGGTG 
TCCAGAGCGA AGGAGCAGAC GTTTATCTTC ACTGATGGGG AGGACAAGGA GCTCCGGCTG 
AAAGCAGGGC TGAACATCAT CAACACCAAC TGCTCGGCGG CTCACACACG CCAAGCCCTG 
TGCTGCAAGA TGTCTGTGGA GTATGACAAG TTCATCGAGT CACAGAAAAA GTGGTTTTGT 
CATGTGGATG ATGATAACTA TGTGATCCTG CCCAGTCTAC TGGAGCTTCT GTCCTCGTAC 
TCTCACACAC AGGATGTGTA TCTGGGCCGG CCTAGCCTGG ATCATCCTAT AGAGGCTGCA 
GAGAGAGTCA AGAGCGACGG ATCGGTGTCA GTGAAGTTCT GGTTTGCCAC TGGTGGAGCT 
GGTTTCTGCA TCAGTCGAGG TTTAGCGCTC AAAATGAGTC CATGGGCCAG TCTTGGAAAC 
TTTATCACAA CAGCAGAGAA GATCCGTCTG CCAGATGACT GTACAATTGG CTACATCATC 
GAGGCGCTGC TGGAGGTTCC TCTCACCCAC ACAGGCCTGT TTCACTCACA CCTGGAGAAC 
CTGCAGAGAC TGCCTGCAGA AAACATCCTG CGACAGGTGA CCCTCAGTTA TGGTGGATTC 
GAGAACAGGA GGAATGTGGT CAGTGTTGGA GGAGCGTTTT CATTGGCTGA AGACCCAACA 
CGGTTTAAAA CGGTTCACTG CAAGCTCTAC CCAGACACCG AGTGGTGCCC ACGCAAGACT 
CGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development.
Neuron94(2)322-336.e5(2017 Apr)
Thomas-Jinu S,Gordon PM,Fielding T,Taylor R,Smith BN,Snowden V,Blanc E,Vance C,Topp S,Wong CH,Bielen H,Williams KL,McCann EP,Nicholson GA,Pan-Vazquez A,Fox AH,Bond CS,Talbot WS,Blair IP,Shaw CE,Houart C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition.
Developmental biology410(2)178-189(2016 Feb)
Chang J,Skromne I,Ho RK