klhl31 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	klhl31
Entrez Gene ID	407079
Full Name	kelch-like family member 31
Synonyms	kbtbd1,klhl,wu:fa31h10,wu:fb95e08
General protein information	Preferred Names kelch-like family member 31 Names kelch-like protein 31 kelch repeat and BTB (POZ) domain containing 1 kelch repeat and BTB domain-containing protein 1 kelch-like 31 kelch-like protein Klhl kelchlike
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156491.4	XP_005156548.1	kelch-like protein 31 isoform X1
NM_001003727.2	NP_001003727.1	kelch-like protein 31
XM_005156491.3	XP_005156548.1	kelch-like protein 31 isoform X1
NM_001003727.3	NP_001003727.1	kelch-like protein 31

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Klhl31	NP_766513.2
Gallus gallus (chicken)	KLHL31	XP_419909.1
Rattus norvegicus (Norway rat)	Klhl31	NP_001101640.1
Danio rerio (zebrafish)	klhl31	NP_001003727.1
	KLHL31	XP_001109056.1
Homo sapiens (human)	KLHL31	NP_001003760.2
Pan troglodytes (chimpanzee)	KLHL31	XP_001156046.2
Canis lupus familiaris (dog)	KLHL31	XP_538967.2
Xenopus tropicalis (tropical clawed frog)	klhl31	XP_002934899.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of function of myosin chaperones triggers Hsf1-mediated transcriptional response in skeletal muscle cells.
Etard C, Armant O, Roostalu U, Gourain V, Ferg M, Str?hle U
Genome biology16267(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Saltatory evolution of the ectodermal neural cortex gene family at the vertebrate origin.
Feiner N, Murakami Y, Breithut L, Mazan S, Meyer A, Kuraku S
Genome biology and evolution5(8)1485-502(2013)

An integrated approach for the systematic identification and characterization of heart-enriched genes with unknown functions.
Uchida S, Schneider A, Wiesnet M, Jungblut B, Zarjitskaya P, Jenniches K, Kreymborg KG, Seeger W, Braun T
BMC genomics10100(2009 Mar)

A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display conserved expression patterns in skeletal and cardiac muscles.
Wu YL, Gong Z
Gene338(1)75-83(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression pattern of klhl, as well as the presence of the kelch repeats indicates a possible role for Klhl in the organization of striated muscle cytoarchitecture.
Title: A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display conserved expression patterns in skeletal and cardiac muscles.

The following klhl31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klhl31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02547	NM_001003727.2 Latest version!	Danio rerio kelch-like family member 31 (klhl31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa18363	NM_001003727.3 Latest version!	Danio rerio kelch-like family member 31 (klhl31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa18363	XM_005156491.4 Latest version!	Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa18363	XM_005156491.3	Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klhl31 ( NM_001003727.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003727.1 Danio rerio kelch-like family member 31 (klhl31), mRNA.

Price & Availability↑

CloneID	ODa02547
Clone ID Related Accession (Same CDS sequence)	NM_001003727.2
Accession Version	NM_001003727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Danio rerio(zebrafish)
Product	kelch-like protein 31
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC131859.1. On Feb 4, 2007 this sequence version replaced NM_001003727.1. ##Evidence-Data-START## Transcript exon combination :: BC131859.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4476758 [ECO:0000350] ##Evidence-Data-END##

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGTGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG GGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT TAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GATGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATCGATGG CAAGATCTTG GTTAGCGGTG GTTACATAAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003727.1
CDS	82..1989
Translation	MAPKKNKAAKKSKADINEMTIMVEDSPSNKINGLNTLLEGGNGFSCISTEVTDPVYAPNLLEGLGHMRQDSFLCDLTVATKSKSFDVHKVVMASCSEYIQNMLRKDPSLKKIELSDLSPVGLATVITYAYSGKLTLSLYTIGSTISAALLLQIHTLVKMCSDFLMRETSVENCMYVVNIADTYNLKETKEAAQKFMRENFIEFSEMEQFLKLTYEQINEFLTDDSLQLPSELTAFQIAVKWLDFDEKRLKYAPDLLSNIRFGTITPQDLVSHVQNVPRMMQDAECHRLLVDAMNYHLLPFQQNILQSRRTKVRGGLRVLLTVGGRPALTEKSLSKDILYRDEDNVWNKLTEMPAKSFNQCVAVLDGFLYVAGGEDQNDARNQAKHAVSNFSRYDPRFNTWIHLANMIQKRTHFSLNTFNGLLFAVGGRNSDGCQASVECYVPSSNQWQMKAPMEVPRCCHASSVIDGKILVSGGYINNAYSRAVCSYDPSTDSWQDKNSLSSPRGWHCSVTVGDRAYVLGGSQLGGRGERVDVLPVECYNPHSGQWSYVAPLLTGVSTAGAATLNNKIYLLGGWNEIEKKYKKCIQVYNPDLNEWTEDDELPEATVGISCCVVTIPTRKTRESRASSVSSAPVSI

Target ORF information:

RefSeq Version	NM_001003727.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kelch-like family member 31 (klhl31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003727.2

ORF Insert Sequence:

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGTGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG GGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT TAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GATGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATCGATGG CAAGATCTTG GTTAGCGGTG GTTACATAAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klhl31 ( NM_001003727.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003727.1 Danio rerio kelch-like family member 31 (klhl31), mRNA.

Price & Availability↑

CloneID	ODa18363
Clone ID Related Accession (Same CDS sequence)	XM_005156491.4 , XM_005156491.3 , NM_001003727.3
Accession Version	NM_001003727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	kelch-like protein 31
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR376766.8. On or before Apr 18, 2018 this sequence version replaced XM_005156491.4, NM_001003727.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: BC131859.1, GFIL01014084.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003727.1
CDS	100..2007
Translation	MAPKKNKAAKKSKADINEMTIMVEDSPSNKINGLNTLLEGGNGFSCISTEVTDPVYAPNLLEGLGHMRQDSFLCDLTVATKSKSFDVHKVVMASCSEYIQNMLRKDPSLKKIELSDLSPVGLATVITYAYSGKLTLSLYTIGSTISAALLLQIHTLVKMCSDFLMRETSVENCMYVVNIADTYNLKETKEAAQKFMRENFIEFSEMEQFLKLTYEQINEFLTDDSLQLPSELTAFQIAVKWLDFDEKRLKYAPDLLSNIRFGTITPQDLVSHVQNVPRMMQDAECHRLLVDAMNYHLLPFQQNILQSRRTKVRGGLRVLLTVGGRPALTEKSLSKDILYRDEDNVWNKLTEMPAKSFNQCVAVLDGFLYVAGGEDQNDARNQAKHAVSNFSRYDPRFNTWIHLANMIQKRTHFSLNTFNGLLFAVGGRNSDGCQASVECYVPSSNQWQMKAPMEVPRCCHASSVIDGKILVSGGYINNAYSRAVCSYDPSTDSWQDKNSLSSPRGWHCSVTVGDRAYVLGGSQLGGRGERVDVLPVECYNPHSGQWSYVAPLLTGVSTAGAATLNNKIYLLGGWNEIEKKYKKCIQVYNPDLNEWTEDDELPEATVGISCCVVTIPTRKTRESRASSVSSAPVSI

Target ORF information:

RefSeq Version	NM_001003727.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kelch-like family member 31 (klhl31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003727.3

ORF Insert Sequence:

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klhl31 ( XM_005156491.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156548.1 Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18363
Clone ID Related Accession (Same CDS sequence)	XM_005156491.4 , XM_005156491.3 , NM_001003727.3
Accession Version	XM_005156491.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kelch-like protein 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156491.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156548.1
CDS	148..2055
Translation	MAPKKNKAAKKSKADINEMTIMVEDSPSNKINGLNTLLEGGNGFSCISTEVTDPVYAPNLLEGLGHMRQDSFLCDLTVATKSKSFDVHKVVMASCSEYIQNMLRKDPSLKKIELSDLSPVGLATVITYAYSGKLTLSLYTIGSTISAALLLQIHTLVKMCSDFLMRETSVENCMYVVNIADTYNLKETKEAAQKFMRENFIEFSEMEQFLKLTYEQINEFLTDDSLQLPSELTAFQIAVKWLDFDEKRLKYAPDLLSNIRFGTITPQDLVSHVQNVPRMMQDAECHRLLVDAMNYHLLPFQQNILQSRRTKVRGGLRVLLTVGGRPALTEKSLSKDILYRDEDNVWNKLTEMPAKSFNQCVAVLDGFLYVAGGEDQNDARNQAKHAVSNFSRYDPRFNTWIHLANMIQKRTHFSLNTFNGLLFAVGGRNSDGCQASVECYVPSSNQWQMKAPMEVPRCCHASSVIDGKILVSGGYINNAYSRAVCSYDPSTDSWQDKNSLSSPRGWHCSVTVGDRAYVLGGSQLGGRGERVDVLPVECYNPHSGQWSYVAPLLTGVSTAGAATLNNKIYLLGGWNEIEKKYKKCIQVYNPDLNEWTEDDELPEATVGISCCVVTIPTRKTRESRASSVSSAPVSI

Target ORF information:

RefSeq Version	XM_005156491.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156491.4

ORF Insert Sequence:

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klhl31 ( XM_005156491.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156548.1 Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.

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CloneID	ODa18363
Clone ID Related Accession (Same CDS sequence)	XM_005156491.4 , XM_005156491.3 , NM_001003727.3
Accession Version	XM_005156491.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kelch-like protein 31 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156548.1
CDS	127..2034
Translation	MAPKKNKAAKKSKADINEMTIMVEDSPSNKINGLNTLLEGGNGFSCISTEVTDPVYAPNLLEGLGHMRQDSFLCDLTVATKSKSFDVHKVVMASCSEYIQNMLRKDPSLKKIELSDLSPVGLATVITYAYSGKLTLSLYTIGSTISAALLLQIHTLVKMCSDFLMRETSVENCMYVVNIADTYNLKETKEAAQKFMRENFIEFSEMEQFLKLTYEQINEFLTDDSLQLPSELTAFQIAVKWLDFDEKRLKYAPDLLSNIRFGTITPQDLVSHVQNVPRMMQDAECHRLLVDAMNYHLLPFQQNILQSRRTKVRGGLRVLLTVGGRPALTEKSLSKDILYRDEDNVWNKLTEMPAKSFNQCVAVLDGFLYVAGGEDQNDARNQAKHAVSNFSRYDPRFNTWIHLANMIQKRTHFSLNTFNGLLFAVGGRNSDGCQASVECYVPSSNQWQMKAPMEVPRCCHASSVIDGKILVSGGYINNAYSRAVCSYDPSTDSWQDKNSLSSPRGWHCSVTVGDRAYVLGGSQLGGRGERVDVLPVECYNPHSGQWSYVAPLLTGVSTAGAATLNNKIYLLGGWNEIEKKYKKCIQVYNPDLNEWTEDDELPEATVGISCCVVTIPTRKTRESRASSVSSAPVSI

Target ORF information:

RefSeq Version	XM_005156491.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kelch-like family member 31 (klhl31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156491.3

ORF Insert Sequence:

ATGGCACCCA AAAAGAACAA GGCGGCTAAG AAGAGCAAAG CCGATATCAA CGAGATGACG 
ATCATGGTCG AGGACAGCCC CTCCAACAAA ATCAACGGGC TCAACACGCT CCTGGAGGGC 
GGAAACGGCT TTAGTTGCAT CTCCACCGAA GTCACCGACC CCGTCTATGC ACCAAACCTC 
CTGGAGGGTC TGGGCCACAT GAGGCAGGAC AGCTTCCTCT GTGACCTCAC GGTAGCAACC 
AAATCCAAGT CCTTCGACGT TCACAAAGTA GTGATGGCAT CCTGCAGCGA GTACATCCAA 
AACATGCTCC GGAAGGATCC GTCTCTAAAG AAGATTGAGC TCAGTGATTT ATCCCCAGTT 
GGTTTGGCTA CAGTCATCAC TTATGCCTAT TCTGGAAAAC TGACCCTGTC TCTATACACC 
ATCGGCAGCA CTATATCTGC AGCCTTGCTC CTCCAGATCC ACACTTTGGT GAAAATGTGT 
AGTGATTTCT TAATGCGGGA GACTAGCGTG GAGAATTGCA TGTATGTGGT CAACATTGCC 
GACACGTACA ATCTAAAAGA GACGAAGGAA GCTGCTCAGA AGTTCATGCG AGAGAACTTC 
ATTGAGTTCT CCGAGATGGA GCAGTTCCTC AAACTCACCT ACGAGCAAAT CAACGAGTTC 
CTCACAGACG ACTCACTTCA GTTGCCTTCA GAGCTCACGG CTTTCCAGAT CGCAGTCAAG 
TGGTTGGATT TTGATGAAAA GAGGTTGAAG TACGCTCCTG ATCTGCTGTC CAACATCCGT 
TTTGGCACCA TCACCCCCCA GGATCTTGTT AGTCACGTGC AGAACGTTCC CAGGATGATG 
CAGGATGCCG AGTGCCACCG TTTGCTGGTC GACGCCATGA ATTACCACCT GCTGCCGTTC 
CAGCAGAACA TCCTTCAATC CCGGAGGACC AAAGTTCGTG GAGGTCTCCG AGTTCTGCTT 
ACTGTTGGCG GACGGCCTGC GCTGACCGAA AAGTCTCTCA GCAAGGACAT TCTCTACAGG 
GACGAGGATA ATGTCTGGAA CAAGCTGACG GAGATGCCTG CTAAGAGCTT CAATCAGTGT 
GTGGCCGTTT TGGACGGTTT CCTTTACGTG GCTGGAGGAG AAGACCAGAA TGATGCAAGA 
AACCAGGCAA AGCATGCAGT CAGCAATTTC AGCAGATACG ACCCCCGATT CAACACGTGG 
ATCCACCTAG CCAACATGAT TCAGAAGCGT ACTCATTTCA GCCTCAACAC CTTCAATGGT 
CTGCTCTTCG CCGTCGGGGG CCGTAATTCT GACGGCTGCC AGGCGTCTGT CGAGTGCTAC 
GTCCCATCCT CAAACCAGTG GCAAATGAAA GCCCCAATGG AAGTCCCTAG ATGCTGCCAT 
GCCAGCTCAG TTATTGATGG CAAGATCTTG GTTAGCGGTG GTTACATTAA CAACGCCTAC 
TCTCGAGCCG TCTGTTCCTA CGACCCATCC ACTGATAGCT GGCAGGATAA AAACAGCCTG 
AGCAGCCCGA GAGGATGGCA CTGTTCGGTG ACCGTCGGAG ATCGTGCTTA CGTGCTCGGC 
GGCAGTCAAC TGGGCGGACG TGGAGAGAGA GTAGACGTCT TGCCTGTTGA ATGCTATAAC 
CCTCACTCTG GCCAGTGGAG CTACGTTGCC CCCTTGCTGA CGGGAGTGAG CACTGCAGGC 
GCTGCCACCT TGAATAACAA GATCTACCTC TTGGGCGGCT GGAATGAGAT TGAGAAGAAG 
TACAAGAAAT GCATTCAGGT TTATAATCCT GATCTTAACG AATGGACTGA AGATGACGAA 
TTGCCAGAGG CTACGGTTGG TATCTCGTGT TGTGTCGTCA CCATCCCCAC ACGCAAAACA 
CGAGAGTCGA GGGCCAGCTC GGTGTCATCC GCACCAGTTA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of function of myosin chaperones triggers Hsf1-mediated transcriptional response in skeletal muscle cells.
Genome biology16267(2015 Dec)
Etard C,Armant O,Roostalu U,Gourain V,Ferg M,Str?hle U

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Saltatory evolution of the ectodermal neural cortex gene family at the vertebrate origin.
Genome biology and evolution5(8)1485-502(2013)
Feiner N,Murakami Y,Breithut L,Mazan S,Meyer A,Kuraku S

An integrated approach for the systematic identification and characterization of heart-enriched genes with unknown functions.
BMC genomics10100(2009 Mar)
Uchida S,Schneider A,Wiesnet M,Jungblut B,Zarjitskaya P,Jenniches K,Kreymborg KG,Seeger W,Braun T

A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display conserved expression patterns in skeletal and cardiac muscles.
Gene338(1)75-83(2004 Aug)
Wu YL,Gong Z

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klhl31 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム