wfikkn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wfikkn1
Entrez Gene ID	406570
Full Name	WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1
Synonyms	Gasp2,wfikkn,zgc:85816
General protein information	Preferred Names WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 Names WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214747.1	NP_999912.1	WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wfikkn1	NP_001093924.1
Bos taurus (cattle)	WFIKKN1	NP_001192725.1
Xenopus tropicalis (tropical clawed frog)	wfikkn1	XP_002932495.1
	WFIKKN1	XP_001118498.2
Canis lupus familiaris (dog)	WFIKKN1	XP_547215.4
Rattus norvegicus (Norway rat)	Wfikkn1	NP_001123248.1
Gallus gallus (chicken)	WFIKKN1	XP_426858.2
Danio rerio (zebrafish)	wfikkn1	NP_999912.1
Homo sapiens (human)	WFIKKN1	NP_444514.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Ligand-Specific Transcriptional Mechanisms Underlie Aryl Hydrocarbon Receptor-Mediated Developmental Toxicity of Oxygenated PAHs.
Goodale BC, La Du J, Tilton SC, Sullivan CM, Bisson WH, Waters KM, Tanguay RL
Toxicological sciences : an official journal of the Society of Toxicology147(2)397-411(2015 Oct)

GASP/WFIKKN proteins: evolutionary aspects of their functions.
Monestier O, Brun C, Cocquempot O, Petit D, Blanquet V
PloS one7(8)e43710(2012)

Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays.
Lindeman LC, Reiner AH, Mathavan S, Alestr?m P, Collas P
PloS one5(12)e15651(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following wfikkn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wfikkn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37596	NM_214747.1 Latest version!	Danio rerio WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 (wfikkn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wfikkn1 ( NM_214747.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999912.1 Danio rerio WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 (wfikkn1), mRNA.

Price & Availability↑

CloneID	ODa37596
Clone ID Related Accession (Same CDS sequence)	NM_214747.1
Accession Version	NM_214747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Danio rerio(zebrafish)
Product	WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068398.1. ##Evidence-Data-START## CDS exon combination :: BC068398.1 [ECO:0000331] ##Evidence-Data-END##

ATGCACTCCT CTGCACTCCT CGTCCTCCTG TGTTTGTGGC CCGCGGCGGC GCTCGGACCC 
TCGGACGCGG ACCATGCCGG AGTCTGTCCC AACCAGCTGA ACCTGCACCT GTGGGTGGAC 
GCACAGAGCA CCTGCGAGAG GGAGTGCCAC AGTGACCAGG ACTGCTCCTC CTCTGAGAAG 
TGCTGCACTA ATGTGTGTGG TCTGCTGAGC TGCGTGGCGT CCCGTGTCTC TGACGGCTCC 
TCTGAGGGTC AGTGGGCCGC TGGTGGGTCG GGGGGCAGCG CTGTGTCCTG CGAGGGGTTC 
GAGTGCCGCC AGCAGGGGGC GGTGTGTGAA CTGTGGGAAG GCCAGCCGGT GTGTAAGTGT 
CAGGACCTCT GCGGGAGTGA GCCCAGCTTC ACCTGTGCCT CTGACGGACT CACCTACTTC 
AACCGCTGCT ACATGGACGC GGAGGCCTGT CTTCAGGGGG TGGAGCTCTC AGAAGTCACC 
TGCCGGTTCC ACCTGTCCAG CCCAAACTCC AGCCCACTGC CGCAGGACCC CACGCCTCCA 
CCTGCGCCGA CAGACGCAGA CACCCAGACC TCCGCTCCCA CCCTGTACTC CAGCCCACAG 
CAGCAGGTCA CTTACCTGGG CGGCACCGTC AGCTTCCACT GCGATGTGAT TGGCCAGCCG 
AAGCCAGAGG TCACCTGGGA GAAGCAGTCG GATGAGCAGG AGCAGGTCGT CATGCGGGCC 
GATCAGATGT TCGGGAACGT GGTGATAACC AGCATCGGGC AGCTGGTGGT GTATAACGCG 
CAGGTGTGGG ACAGCGGGAT CTACAGCTGT GTGGCGCGGA ACTCTGCGGG CGTCCTGCGG 
GCCGACTTCT CGCTGTCCGT GGTCAGCCAT GCGGATCAGG ACTTCTTCGA TGACCCTGCA 
GCGGGCCTGC CTCTGGGACG GCCATTCTCG CCAGCAGACT GCTCTGCGGC TGTGGAGCGC 
GGCGACTGCG GAGAGAAGCG TGTGGACTGG TTCTTCGATC CCGCCCGAGG CTCCTGCCAC 
ACATTCACAC ACGGAGGCTG CGAGGGCCGC AACCGCTTCC ACACCTTCGA GGAGTGCCGG 
GCGTCCTGTC AGCGCGAGGG GCAGGCCGTG TGCTCACTGC CCGCCGTGCA GGGACCCTGC 
CGACACTGGC AGGCGCGCTG GTTCTACAAC AGCCTGACGG AGCGATGCGA GGCTTTCCTG 
TACGGCGGCT GCAGCGGCAA CAAGAACAGC TTCGGCACGC GGCGCGAGTG TGACGCGCAC 
TGCCCAACAC ACCGACCGCG GCCCTGCAGG AGCTGCCGGC ACAGGGGACG CATCATGGAC 
ACGCTCTGCA GCAGCGACTT CGCCATAGTG GGCCAGCTCA CAGAACTGAT CCAGGAGCTG 
GACTCGGGAA TGGCGCGGTT CCACCTGCAG CAGGTGTTGC GGGACGAGAA GATGGGCCTG 
CAGCTCTTCA GGACACAACA CCTGGAGGTG CTGCTGCCAC AGGTGGACTG GAGCTGCCCC 
TGCCCCAACA TCACACAGCT CCAGCTCCCC CTGCTGGTGA TGGGGGTGGT GCAGGACGGG 
GCGGCCATAC TGCTGCCCCA CAGCTACGCC CGGCCCGTCT CTGAGCGCCG CCTGATGAAG 
ATCCACGAGG CTCTGGACAA GAACATCTGC GGGACGCTGA GGAGACTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999912.1
CDS	20..1696
Translation	MHSSALLVLLCLWPAAALGPSDADHAGVCPNQLNLHLWVDAQSTCERECHSDQDCSSSEKCCTNVCGLLSCVASRVSDGSSEGQWAAGGSGGSAVSCEGFECRQQGAVCELWEGQPVCKCQDLCGSEPSFTCASDGLTYFNRCYMDAEACLQGVELSEVTCRFHLSSPNSSPLPQDPTPPPAPTDADTQTSAPTLYSSPQQQVTYLGGTVSFHCDVIGQPKPEVTWEKQSDEQEQVVMRADQMFGNVVITSIGQLVVYNAQVWDSGIYSCVARNSAGVLRADFSLSVVSHADQDFFDDPAAGLPLGRPFSPADCSAAVERGDCGEKRVDWFFDPARGSCHTFTHGGCEGRNRFHTFEECRASCQREGQAVCSLPAVQGPCRHWQARWFYNSLTERCEAFLYGGCSGNKNSFGTRRECDAHCPTHRPRPCRSCRHRGRIMDTLCSSDFAIVGQLTELIQELDSGMARFHLQQVLRDEKMGLQLFRTQHLEVLLPQVDWSCPCPNITQLQLPLLVMGVVQDGAAILLPHSYARPVSERRLMKIHEALDKNICGTLRRLQD

Target ORF information:

RefSeq Version	NM_214747.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 (wfikkn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214747.1

ORF Insert Sequence:

ATGCACTCCT CTGCACTCCT CGTCCTCCTG TGTTTGTGGC CCGCGGCGGC GCTCGGACCC 
TCGGACGCGG ACCATGCCGG AGTCTGTCCC AACCAGCTGA ACCTGCACCT GTGGGTGGAC 
GCACAGAGCA CCTGCGAGAG GGAGTGCCAC AGTGACCAGG ACTGCTCCTC CTCTGAGAAG 
TGCTGCACTA ATGTGTGTGG TCTGCTGAGC TGCGTGGCGT CCCGTGTCTC TGACGGCTCC 
TCTGAGGGTC AGTGGGCCGC TGGTGGGTCG GGGGGCAGCG CTGTGTCCTG CGAGGGGTTC 
GAGTGCCGCC AGCAGGGGGC GGTGTGTGAA CTGTGGGAAG GCCAGCCGGT GTGTAAGTGT 
CAGGACCTCT GCGGGAGTGA GCCCAGCTTC ACCTGTGCCT CTGACGGACT CACCTACTTC 
AACCGCTGCT ACATGGACGC GGAGGCCTGT CTTCAGGGGG TGGAGCTCTC AGAAGTCACC 
TGCCGGTTCC ACCTGTCCAG CCCAAACTCC AGCCCACTGC CGCAGGACCC CACGCCTCCA 
CCTGCGCCGA CAGACGCAGA CACCCAGACC TCCGCTCCCA CCCTGTACTC CAGCCCACAG 
CAGCAGGTCA CTTACCTGGG CGGCACCGTC AGCTTCCACT GCGATGTGAT TGGCCAGCCG 
AAGCCAGAGG TCACCTGGGA GAAGCAGTCG GATGAGCAGG AGCAGGTCGT CATGCGGGCC 
GATCAGATGT TCGGGAACGT GGTGATAACC AGCATCGGGC AGCTGGTGGT GTATAACGCG 
CAGGTGTGGG ACAGCGGGAT CTACAGCTGT GTGGCGCGGA ACTCTGCGGG CGTCCTGCGG 
GCCGACTTCT CGCTGTCCGT GGTCAGCCAT GCGGATCAGG ACTTCTTCGA TGACCCTGCA 
GCGGGCCTGC CTCTGGGACG GCCATTCTCG CCAGCAGACT GCTCTGCGGC TGTGGAGCGC 
GGCGACTGCG GAGAGAAGCG TGTGGACTGG TTCTTCGATC CCGCCCGAGG CTCCTGCCAC 
ACATTCACAC ACGGAGGCTG CGAGGGCCGC AACCGCTTCC ACACCTTCGA GGAGTGCCGG 
GCGTCCTGTC AGCGCGAGGG GCAGGCCGTG TGCTCACTGC CCGCCGTGCA GGGACCCTGC 
CGACACTGGC AGGCGCGCTG GTTCTACAAC AGCCTGACGG AGCGATGCGA GGCTTTCCTG 
TACGGCGGCT GCAGCGGCAA CAAGAACAGC TTCGGCACGC GGCGCGAGTG TGACGCGCAC 
TGCCCAACAC ACCGACCGCG GCCCTGCAGG AGCTGCCGGC ACAGGGGACG CATCATGGAC 
ACGCTCTGCA GCAGCGACTT CGCCATAGTG GGCCAGCTCA CAGAACTGAT CCAGGAGCTG 
GACTCGGGAA TGGCGCGGTT CCACCTGCAG CAGGTGTTGC GGGACGAGAA GATGGGCCTG 
CAGCTCTTCA GGACACAACA CCTGGAGGTG CTGCTGCCAC AGGTGGACTG GAGCTGCCCC 
TGCCCCAACA TCACACAGCT CCAGCTCCCC CTGCTGGTGA TGGGGGTGGT GCAGGACGGG 
GCGGCCATAC TGCTGCCCCA CAGCTACGCC CGGCCCGTCT CTGAGCGCCG CCTGATGAAG 
ATCCACGAGG CTCTGGACAA GAACATCTGC GGGACGCTGA GGAGACTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Ligand-Specific Transcriptional Mechanisms Underlie Aryl Hydrocarbon Receptor-Mediated Developmental Toxicity of Oxygenated PAHs.
Toxicological sciences : an official journal of the Society of Toxicology147(2)397-411(2015 Oct)
Goodale BC,La Du J,Tilton SC,Sullivan CM,Bisson WH,Waters KM,Tanguay RL

GASP/WFIKKN proteins: evolutionary aspects of their functions.
PloS one7(8)e43710(2012)
Monestier O,Brun C,Cocquempot O,Petit D,Blanquet V

Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays.
PloS one5(12)e15651(2010 Dec)
Lindeman LC,Reiner AH,Mathavan S,Alestr?m P,Collas P