tlr5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tlr5a
Entrez Gene ID	403138
Full Name	toll-like receptor 5a
General protein information	Preferred Names toll-like receptor 5a Names toll-like receptor 5 toll-like receptor 5a TIR5a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001919017.5	XP_001919052.2	toll-like receptor 5
XM_001919017.6	XP_001919052.2	toll-like receptor 5

Pathways from BioSystems

Homologies

	TLR5	NP_001123901.1
Canis lupus familiaris (dog)	TLR5	NP_001184105.1
Gallus gallus (chicken)	TLR5	NP_001019757.1
Danio rerio (zebrafish)	tlr5b	NP_001124067.1
Xenopus tropicalis (tropical clawed frog)	LOC100496647	XP_002940742.2
Danio rerio (zebrafish)	tlr5a	XP_001919052.2
Homo sapiens (human)	TLR5	NP_003259.2
Bos taurus (cattle)	TLR5	NP_001035591.1
Pan troglodytes (chimpanzee)	TLR5	NP_001123934.1
Mus musculus (house mouse)	Tlr5	NP_058624.2
Rattus norvegicus (Norway rat)	Tlr5	NP_001139300.1

Gene Ontology

The following tlr5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tlr5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25313	XM_001919017.6 Latest version!	Danio rerio toll-like receptor 5a (tlr5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa25313	XM_001919017.5	Danio rerio toll-like receptor 5a (tlr5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tlr5a ( XM_001919017.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919052.2 Danio rerio toll-like receptor 5a (tlr5a), mRNA.

Price & Availability↑

CloneID	ODa25313
Clone ID Related Accession (Same CDS sequence)	XM_001919017.5 , XM_001919017.6
Accession Version	XM_001919017.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	toll-like receptor 5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919017.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA CATACACTTT ATTTCTGATG CTATTATTCA TTTGGACTCC AACAGTGAAA 
TCCACCTCAG TGTGTTCAAC TGATGGCTAC TTCGCTTTCT GCATGGACAG AGGTCTTCAA 
GAGGTGCCAA AGATTTCCAC TTACATAACT AACGTGGATT TGAGTAAAAA CAGAATTGCT 
GAACTCAATG AAACATCCTT TTCTCATTTG GAAGCTCTAG AAGTCCTCAT CCTGATGCAC 
CAAACACCAG GTCTTGTGAT CCGGCGCAAT TCATTCATGA GGCTCTCTAA TTTAACATCA 
CTTCAGCTAG ACTACAACCA CCACCTGCGA ATAGATGCAG GAGCATTTAA TGGATTATCC 
GACCTTAAAA ATCTCACTCT CACTCAATGC GGTTTAGATG ACTCCCTACT GTCTGGTAAC 
TTCCTCAAAC CTCTGATGTC TCTTGAGATG CTTGATTTAA GTCGTAACAA CATTCGGAGA 
GTCCAACCGG CGTCTTTCTT TTTAAATATG AGGCGATTTC ATGTGCTGGA TCTGACTTTC 
AACAAAGTGA AGAGCATCTG TGAGGAAGAC CTCCTGAACT TTCAGGGTAA ACATTTCACA 
CTCCTCAGAC TGTCCTCTAT AACACTGCAA GACATGAATG AGTACTGGTT AGGATGGGAG 
AAATGTGGAA ACCCATTTAA GAATACTTCA ATAACCACAT TGGATCTATC TGGAAACGGC 
TTTAACGTTG ACATGGCTAA GAGATTTTTT GATGCTATCT CCAGTACCAA AATCCAAAGT 
CTCATCATCA GTAACACTTA CAGAATCGGC AAATCTTCTG GCGATAATTC GAAAGATCCA 
GACAAATCGA CATTCAGTGG TCTTAAGTCG AGCGGTATTA AGAGTTTTGA TCTGTCCAAT 
TCAAGCATTT TTTCTCTCAC ATATTCTGTA TTTAGTTATC TGTCAGATCT AGAACAAATC 
ACATTAGCAG AAAGTCAGAT CAACAAGATT GAAAACAACG CATTTTTGGG TATGACAAAC 
TTGCTAAAGC TAAACCTGTC CAAAAACTTC CTCGGCTACA TTGATTTTAG GACATTTCAG 
AATCTCAAGG GACTCGAGGT GCTTGATTTG TCTTACAATC ATATTTGGAG ACTTGGGTCT 
CAGTCATTTC AAGGTCTTCC AAATCTACTC AGTCTAAACC TAACAGGAAA TTCTCTTGGA 
CATGTATACA CATTCGCAAC TCTGCCGAAG TTGGAAAAGC TCTACTTAGG AGACAACAAC 
ATTCAGTATG TGTACGAAAT ACCCAACATA GCCAAACATC TCAAAACCCT TGACTTGCAG 
TTTAACCAAA TAGCATCCAT GTCAGAGCTC TACACAATAC TAGATGAATT TCCTCAAATT 
GAGGAAGTAG TTTTTCGAGG AAATCAGCTT CTTTATTGCC CTCAAGACAA CCATGAAGTG 
TTATCACAAA ACATACAAGT CCTCGATCTA TCATTTGCAG GTTTGCAAGT AATCTGGTCA 
GAAGGAAAAT GTTTAAACGT GTTTGACGAT CTTCACCAGC TAGAAGTGCT TCATTTGAGT 
TCCAACTTGC TGCAGTCACT TCCCAAAGAC ATCTTCAAAG ACCTTACATC TTTGCTCATT 
CTGGATTTGT CTTTCAACTC TTTGAAATAC CTCCCCACTG ATGTATTCCC CAAAACTCTT 
CAAGTACTTC ATCTTGACTA TAATTCGATT TATTCAGTAG ATCCAAATCT CTTCAGCACC 
CTCAGCTACC TCAGCCTGAT GAATAACGAT TTCCGATGTG ATTGTGACTT GAAGGATTTC 
CAAACATGGC TAAATCAAAC CAACATAACC TTCGTTCACT CTATTGAGGA TGTGACGTGT 
GCCAGTCCTG AGGATCAGTA CATGGTTCCG GTTGTGAGTT CCAGCATACA ATGTGAGGAT 
GAAGAGGAGG AGAGAAGAAC TGAAAAACTA AGACTTGTGC TTTTTATTTC CTGCACTGTA 
TTGATTATAT TATTCAGCGC CAGCACCATT GTTTACATCA GTCGACGCGG TGTCATCTTC 
AAGATATACA AAAAACTAAT TGGCAAATTT GACAATAAAC CTCAAGAGGA ACCTGATGCA 
AATGGATTTT TGTACGATGT GTACCTGTGT TTCAGTTCCA GAGACATGAA GTGGGTAGAA 
AGAGCGTTGT TGAAGAGGCT GGACTCTCAG TTTTCAGAGC ACAACACACT CCGCTGCTGC 
TTCGAGGAGC GAGACTTCAT CCCCGGGGAG GACCATCTTA CCAATATGAG AAATGCCATC 
CAGCATAGTC GAAAAACCAT TTGCGTGGTG TCTAAACACT TTCTGAAGGA CGGCTGGTGT 
CTAGAAACAT TCACCCTGGC ACAATGTAGG ATGCTAGTGG AGCTTAAGGA CATTCTGGTG 
GTGCTGGTTG TAGGAAACAT ACCGCAGTAC AGGTTATTAA AGTATGAACA ACTGAGATCC 
TTCATTGAGA ACAGAAGTTA CCTCGTGTGG CCTGATGACG GTCAAGACTT GGAGTGGTTT 
TATGACCAAC TTCTGCACAA GATAATCAGA AACACCAAGG TTAAACAAAC AAACATTAAA 
GAAAAAGACA AGGTGGATGA AAACAATCCT GAAGCAGCAG ATGTTCAAGC AGACACTGCA 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919052.2
CDS	147..2792
Translation	MAATYTLFLMLLFIWTPTVKSTSVCSTDGYFAFCMDRGLQEVPKISTYITNVDLSKNRIAELNETSFSHLEALEVLILMHQTPGLVIRRNSFMRLSNLTSLQLDYNHHLRIDAGAFNGLSDLKNLTLTQCGLDDSLLSGNFLKPLMSLEMLDLSRNNIRRVQPASFFLNMRRFHVLDLTFNKVKSICEEDLLNFQGKHFTLLRLSSITLQDMNEYWLGWEKCGNPFKNTSITTLDLSGNGFNVDMAKRFFDAISSTKIQSLIISNTYRIGKSSGDNSKDPDKSTFSGLKSSGIKSFDLSNSSIFSLTYSVFSYLSDLEQITLAESQINKIENNAFLGMTNLLKLNLSKNFLGYIDFRTFQNLKGLEVLDLSYNHIWRLGSQSFQGLPNLLSLNLTGNSLGHVYTFATLPKLEKLYLGDNNIQYVYEIPNIAKHLKTLDLQFNQIASMSELYTILDEFPQIEEVVFRGNQLLYCPQDNHEVLSQNIQVLDLSFAGLQVIWSEGKCLNVFDDLHQLEVLHLSSNLLQSLPKDIFKDLTSLLILDLSFNSLKYLPTDVFPKTLQVLHLDYNSIYSVDPNLFSTLSYLSLMNNDFRCDCDLKDFQTWLNQTNITFVHSIEDVTCASPEDQYMVPVVSSSIQCEDEEEERRTEKLRLVLFISCTVLIILFSASTIVYISRRGVIFKIYKKLIGKFDNKPQEEPDANGFLYDVYLCFSSRDMKWVERALLKRLDSQFSEHNTLRCCFEERDFIPGEDHLTNMRNAIQHSRKTICVVSKHFLKDGWCLETFTLAQCRMLVELKDILVVLVVGNIPQYRLLKYEQLRSFIENRSYLVWPDDGQDLEWFYDQLLHKIIRNTKVKQTNIKEKDKVDENNPEAADVQADTAV

Target ORF information:

RefSeq Version	XM_001919017.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio toll-like receptor 5a (tlr5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919017.5

ORF Insert Sequence:

ATGGCAGCTA CATACACTTT ATTTCTGATG CTATTATTCA TTTGGACTCC AACAGTGAAA 
TCCACCTCAG TGTGTTCAAC TGATGGCTAC TTCGCTTTCT GCATGGACAG AGGTCTTCAA 
GAGGTGCCAA AGATTTCCAC TTACATAACT AACGTGGATT TGAGTAAAAA CAGAATTGCT 
GAACTCAATG AAACATCCTT TTCTCATTTG GAAGCTCTAG AAGTCCTCAT CCTGATGCAC 
CAAACACCAG GTCTTGTGAT CCGGCGCAAT TCATTCATGA GGCTCTCTAA TTTAACATCA 
CTTCAGCTAG ACTACAACCA CCACCTGCGA ATAGATGCAG GAGCATTTAA TGGATTATCC 
GACCTTAAAA ATCTCACTCT CACTCAATGC GGTTTAGATG ACTCCCTACT GTCTGGTAAC 
TTCCTCAAAC CTCTGATGTC TCTTGAGATG CTTGATTTAA GTCGTAACAA CATTCGGAGA 
GTCCAACCGG CGTCTTTCTT TTTAAATATG AGGCGATTTC ATGTGCTGGA TCTGACTTTC 
AACAAAGTGA AGAGCATCTG TGAGGAAGAC CTCCTGAACT TTCAGGGTAA ACATTTCACA 
CTCCTCAGAC TGTCCTCTAT AACACTGCAA GACATGAATG AGTACTGGTT AGGATGGGAG 
AAATGTGGAA ACCCATTTAA GAATACTTCA ATAACCACAT TGGATCTATC TGGAAACGGC 
TTTAACGTTG ACATGGCTAA GAGATTTTTT GATGCTATCT CCAGTACCAA AATCCAAAGT 
CTCATCATCA GTAACACTTA CAGAATCGGC AAATCTTCTG GCGATAATTC GAAAGATCCA 
GACAAATCGA CATTCAGTGG TCTTAAGTCG AGCGGTATTA AGAGTTTTGA TCTGTCCAAT 
TCAAGCATTT TTTCTCTCAC ATATTCTGTA TTTAGTTATC TGTCAGATCT AGAACAAATC 
ACATTAGCAG AAAGTCAGAT CAACAAGATT GAAAACAACG CATTTTTGGG TATGACAAAC 
TTGCTAAAGC TAAACCTGTC CAAAAACTTC CTCGGCTACA TTGATTTTAG GACATTTCAG 
AATCTCAAGG GACTCGAGGT GCTTGATTTG TCTTACAATC ATATTTGGAG ACTTGGGTCT 
CAGTCATTTC AAGGTCTTCC AAATCTACTC AGTCTAAACC TAACAGGAAA TTCTCTTGGA 
CATGTATACA CATTCGCAAC TCTGCCGAAG TTGGAAAAGC TCTACTTAGG AGACAACAAC 
ATTCAGTATG TGTACGAAAT ACCCAACATA GCCAAACATC TCAAAACCCT TGACTTGCAG 
TTTAACCAAA TAGCATCCAT GTCAGAGCTC TACACAATAC TAGATGAATT TCCTCAAATT 
GAGGAAGTAG TTTTTCGAGG AAATCAGCTT CTTTATTGCC CTCAAGACAA CCATGAAGTG 
TTATCACAAA ACATACAAGT CCTCGATCTA TCATTTGCAG GTTTGCAAGT AATCTGGTCA 
GAAGGAAAAT GTTTAAACGT GTTTGACGAT CTTCACCAGC TAGAAGTGCT TCATTTGAGT 
TCCAACTTGC TGCAGTCACT TCCCAAAGAC ATCTTCAAAG ACCTTACATC TTTGCTCATT 
CTGGATTTGT CTTTCAACTC TTTGAAATAC CTCCCCACTG ATGTATTCCC CAAAACTCTT 
CAAGTACTTC ATCTTGACTA TAATTCGATT TATTCAGTAG ATCCAAATCT CTTCAGCACC 
CTCAGCTACC TCAGCCTGAT GAATAACGAT TTCCGATGTG ATTGTGACTT GAAGGATTTC 
CAAACATGGC TAAATCAAAC CAACATAACC TTCGTTCACT CTATTGAGGA TGTGACGTGT 
GCCAGTCCTG AGGATCAGTA CATGGTTCCG GTTGTGAGTT CCAGCATACA ATGTGAGGAT 
GAAGAGGAGG AGAGAAGAAC TGAAAAACTA AGACTTGTGC TTTTTATTTC CTGCACTGTA 
TTGATTATAT TATTCAGCGC CAGCACCATT GTTTACATCA GTCGACGCGG TGTCATCTTC 
AAGATATACA AAAAACTAAT TGGCAAATTT GACAATAAAC CTCAAGAGGA ACCTGATGCA 
AATGGATTTT TGTACGATGT GTACCTGTGT TTCAGTTCCA GAGACATGAA GTGGGTAGAA 
AGAGCGTTGT TGAAGAGGCT GGACTCTCAG TTTTCAGAGC ACAACACACT CCGCTGCTGC 
TTCGAGGAGC GAGACTTCAT CCCCGGGGAG GACCATCTTA CCAATATGAG AAATGCCATC 
CAGCATAGTC GAAAAACCAT TTGCGTGGTG TCTAAACACT TTCTGAAGGA CGGCTGGTGT 
CTAGAAACAT TCACCCTGGC ACAATGTAGG ATGCTAGTGG AGCTTAAGGA CATTCTGGTG 
GTGCTGGTTG TAGGAAACAT ACCGCAGTAC AGGTTATTAA AGTATGAACA ACTGAGATCC 
TTCATTGAGA ACAGAAGTTA CCTCGTGTGG CCTGATGACG GTCAAGACTT GGAGTGGTTT 
TATGACCAAC TTCTGCACAA GATAATCAGA AACACCAAGG TTAAACAAAC AAACATTAAA 
GAAAAAGACA AGGTGGATGA AAACAATCCT GAAGCAGCAG ATGTTCAAGC AGACACTGCA 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlr5a ( XM_001919017.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919052.2 Danio rerio toll-like receptor 5a (tlr5a), mRNA.

Price & Availability↑

CloneID	ODa25313
Clone ID Related Accession (Same CDS sequence)	XM_001919017.5 , XM_001919017.6
Accession Version	XM_001919017.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	toll-like receptor 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919017.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA CATACACTTT ATTTCTGATG CTATTATTCA TTTGGACTCC AACAGTGAAA 
TCCACCTCAG TGTGTTCAAC TGATGGCTAC TTCGCTTTCT GCATGGACAG AGGTCTTCAA 
GAGGTGCCAA AGATTTCCAC TTACATAACT AACGTGGATT TGAGTAAAAA CAGAATTGCT 
GAACTCAATG AAACATCCTT TTCTCATTTG GAAGCTCTAG AAGTCCTCAT CCTGATGCAC 
CAAACACCAG GTCTTGTGAT CCGGCGCAAT TCATTCATGA GGCTCTCTAA TTTAACATCA 
CTTCAGCTAG ACTACAACCA CCACCTGCGA ATAGATGCAG GAGCATTTAA TGGATTATCC 
GACCTTAAAA ATCTCACTCT CACTCAATGC GGTTTAGATG ACTCCCTACT GTCTGGTAAC 
TTCCTCAAAC CTCTGATGTC TCTTGAGATG CTTGATTTAA GTCGTAACAA CATTCGGAGA 
GTCCAACCGG CGTCTTTCTT TTTAAATATG AGGCGATTTC ATGTGCTGGA TCTGACTTTC 
AACAAAGTGA AGAGCATCTG TGAGGAAGAC CTCCTGAACT TTCAGGGTAA ACATTTCACA 
CTCCTCAGAC TGTCCTCTAT AACACTGCAA GACATGAATG AGTACTGGTT AGGATGGGAG 
AAATGTGGAA ACCCATTTAA GAATACTTCA ATAACCACAT TGGATCTATC TGGAAACGGC 
TTTAACGTTG ACATGGCTAA GAGATTTTTT GATGCTATCT CCAGTACCAA AATCCAAAGT 
CTCATCATCA GTAACACTTA CAGAATCGGC AAATCTTCTG GCGATAATTC GAAAGATCCA 
GACAAATCGA CATTCAGTGG TCTTAAGTCG AGCGGTATTA AGAGTTTTGA TCTGTCCAAT 
TCAAGCATTT TTTCTCTCAC ATATTCTGTA TTTAGTTATC TGTCAGATCT AGAACAAATC 
ACATTAGCAG AAAGTCAGAT CAACAAGATT GAAAACAACG CATTTTTGGG TATGACAAAC 
TTGCTAAAGC TAAACCTGTC CAAAAACTTC CTCGGCTACA TTGATTTTAG GACATTTCAG 
AATCTCAAGG GACTCGAGGT GCTTGATTTG TCTTACAATC ATATTTGGAG ACTTGGGTCT 
CAGTCATTTC AAGGTCTTCC AAATCTACTC AGTCTAAACC TAACAGGAAA TTCTCTTGGA 
CATGTATACA CATTCGCAAC TCTGCCGAAG TTGGAAAAGC TCTACTTAGG AGACAACAAC 
ATTCAGTATG TGTACGAAAT ACCCAACATA GCCAAACATC TCAAAACCCT TGACTTGCAG 
TTTAACCAAA TAGCATCCAT GTCAGAGCTC TACACAATAC TAGATGAATT TCCTCAAATT 
GAGGAAGTAG TTTTTCGAGG AAATCAGCTT CTTTATTGCC CTCAAGACAA CCATGAAGTG 
TTATCACAAA ACATACAAGT CCTCGATCTA TCATTTGCAG GTTTGCAAGT AATCTGGTCA 
GAAGGAAAAT GTTTAAACGT GTTTGACGAT CTTCACCAGC TAGAAGTGCT TCATTTGAGT 
TCCAACTTGC TGCAGTCACT TCCCAAAGAC ATCTTCAAAG ACCTTACATC TTTGCTCATT 
CTGGATTTGT CTTTCAACTC TTTGAAATAC CTCCCCACTG ATGTATTCCC CAAAACTCTT 
CAAGTACTTC ATCTTGACTA TAATTCGATT TATTCAGTAG ATCCAAATCT CTTCAGCACC 
CTCAGCTACC TCAGCCTGAT GAATAACGAT TTCCGATGTG ATTGTGACTT GAAGGATTTC 
CAAACATGGC TAAATCAAAC CAACATAACC TTCGTTCACT CTATTGAGGA TGTGACGTGT 
GCCAGTCCTG AGGATCAGTA CATGGTTCCG GTTGTGAGTT CCAGCATACA ATGTGAGGAT 
GAAGAGGAGG AGAGAAGAAC TGAAAAACTA AGACTTGTGC TTTTTATTTC CTGCACTGTA 
TTGATTATAT TATTCAGCGC CAGCACCATT GTTTACATCA GTCGACGCGG TGTCATCTTC 
AAGATATACA AAAAACTAAT TGGCAAATTT GACAATAAAC CTCAAGAGGA ACCTGATGCA 
AATGGATTTT TGTACGATGT GTACCTGTGT TTCAGTTCCA GAGACATGAA GTGGGTAGAA 
AGAGCGTTGT TGAAGAGGCT GGACTCTCAG TTTTCAGAGC ACAACACACT CCGCTGCTGC 
TTCGAGGAGC GAGACTTCAT CCCCGGGGAG GACCATCTTA CCAATATGAG AAATGCCATC 
CAGCATAGTC GAAAAACCAT TTGCGTGGTG TCTAAACACT TTCTGAAGGA CGGCTGGTGT 
CTAGAAACAT TCACCCTGGC ACAATGTAGG ATGCTAGTGG AGCTTAAGGA CATTCTGGTG 
GTGCTGGTTG TAGGAAACAT ACCGCAGTAC AGGTTATTAA AGTATGAACA ACTGAGATCC 
TTCATTGAGA ACAGAAGTTA CCTCGTGTGG CCTGATGACG GTCAAGACTT GGAGTGGTTT 
TATGACCAAC TTCTGCACAA GATAATCAGA AACACCAAGG TTAAACAAAC AAACATTAAA 
GAAAAAGACA AGGTGGATGA AAACAATCCT GAAGCAGCAG ATGTTCAAGC AGACACTGCA 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919052.2
CDS	689..3334
Translation	MAATYTLFLMLLFIWTPTVKSTSVCSTDGYFAFCMDRGLQEVPKISTYITNVDLSKNRIAELNETSFSHLEALEVLILMHQTPGLVIRRNSFMRLSNLTSLQLDYNHHLRIDAGAFNGLSDLKNLTLTQCGLDDSLLSGNFLKPLMSLEMLDLSRNNIRRVQPASFFLNMRRFHVLDLTFNKVKSICEEDLLNFQGKHFTLLRLSSITLQDMNEYWLGWEKCGNPFKNTSITTLDLSGNGFNVDMAKRFFDAISSTKIQSLIISNTYRIGKSSGDNSKDPDKSTFSGLKSSGIKSFDLSNSSIFSLTYSVFSYLSDLEQITLAESQINKIENNAFLGMTNLLKLNLSKNFLGYIDFRTFQNLKGLEVLDLSYNHIWRLGSQSFQGLPNLLSLNLTGNSLGHVYTFATLPKLEKLYLGDNNIQYVYEIPNIAKHLKTLDLQFNQIASMSELYTILDEFPQIEEVVFRGNQLLYCPQDNHEVLSQNIQVLDLSFAGLQVIWSEGKCLNVFDDLHQLEVLHLSSNLLQSLPKDIFKDLTSLLILDLSFNSLKYLPTDVFPKTLQVLHLDYNSIYSVDPNLFSTLSYLSLMNNDFRCDCDLKDFQTWLNQTNITFVHSIEDVTCASPEDQYMVPVVSSSIQCEDEEEERRTEKLRLVLFISCTVLIILFSASTIVYISRRGVIFKIYKKLIGKFDNKPQEEPDANGFLYDVYLCFSSRDMKWVERALLKRLDSQFSEHNTLRCCFEERDFIPGEDHLTNMRNAIQHSRKTICVVSKHFLKDGWCLETFTLAQCRMLVELKDILVVLVVGNIPQYRLLKYEQLRSFIENRSYLVWPDDGQDLEWFYDQLLHKIIRNTKVKQTNIKEKDKVDENNPEAADVQADTAV

Target ORF information:

RefSeq Version	XM_001919017.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio toll-like receptor 5a (tlr5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919017.6

ORF Insert Sequence:

ATGGCAGCTA CATACACTTT ATTTCTGATG CTATTATTCA TTTGGACTCC AACAGTGAAA 
TCCACCTCAG TGTGTTCAAC TGATGGCTAC TTCGCTTTCT GCATGGACAG AGGTCTTCAA 
GAGGTGCCAA AGATTTCCAC TTACATAACT AACGTGGATT TGAGTAAAAA CAGAATTGCT 
GAACTCAATG AAACATCCTT TTCTCATTTG GAAGCTCTAG AAGTCCTCAT CCTGATGCAC 
CAAACACCAG GTCTTGTGAT CCGGCGCAAT TCATTCATGA GGCTCTCTAA TTTAACATCA 
CTTCAGCTAG ACTACAACCA CCACCTGCGA ATAGATGCAG GAGCATTTAA TGGATTATCC 
GACCTTAAAA ATCTCACTCT CACTCAATGC GGTTTAGATG ACTCCCTACT GTCTGGTAAC 
TTCCTCAAAC CTCTGATGTC TCTTGAGATG CTTGATTTAA GTCGTAACAA CATTCGGAGA 
GTCCAACCGG CGTCTTTCTT TTTAAATATG AGGCGATTTC ATGTGCTGGA TCTGACTTTC 
AACAAAGTGA AGAGCATCTG TGAGGAAGAC CTCCTGAACT TTCAGGGTAA ACATTTCACA 
CTCCTCAGAC TGTCCTCTAT AACACTGCAA GACATGAATG AGTACTGGTT AGGATGGGAG 
AAATGTGGAA ACCCATTTAA GAATACTTCA ATAACCACAT TGGATCTATC TGGAAACGGC 
TTTAACGTTG ACATGGCTAA GAGATTTTTT GATGCTATCT CCAGTACCAA AATCCAAAGT 
CTCATCATCA GTAACACTTA CAGAATCGGC AAATCTTCTG GCGATAATTC GAAAGATCCA 
GACAAATCGA CATTCAGTGG TCTTAAGTCG AGCGGTATTA AGAGTTTTGA TCTGTCCAAT 
TCAAGCATTT TTTCTCTCAC ATATTCTGTA TTTAGTTATC TGTCAGATCT AGAACAAATC 
ACATTAGCAG AAAGTCAGAT CAACAAGATT GAAAACAACG CATTTTTGGG TATGACAAAC 
TTGCTAAAGC TAAACCTGTC CAAAAACTTC CTCGGCTACA TTGATTTTAG GACATTTCAG 
AATCTCAAGG GACTCGAGGT GCTTGATTTG TCTTACAATC ATATTTGGAG ACTTGGGTCT 
CAGTCATTTC AAGGTCTTCC AAATCTACTC AGTCTAAACC TAACAGGAAA TTCTCTTGGA 
CATGTATACA CATTCGCAAC TCTGCCGAAG TTGGAAAAGC TCTACTTAGG AGACAACAAC 
ATTCAGTATG TGTACGAAAT ACCCAACATA GCCAAACATC TCAAAACCCT TGACTTGCAG 
TTTAACCAAA TAGCATCCAT GTCAGAGCTC TACACAATAC TAGATGAATT TCCTCAAATT 
GAGGAAGTAG TTTTTCGAGG AAATCAGCTT CTTTATTGCC CTCAAGACAA CCATGAAGTG 
TTATCACAAA ACATACAAGT CCTCGATCTA TCATTTGCAG GTTTGCAAGT AATCTGGTCA 
GAAGGAAAAT GTTTAAACGT GTTTGACGAT CTTCACCAGC TAGAAGTGCT TCATTTGAGT 
TCCAACTTGC TGCAGTCACT TCCCAAAGAC ATCTTCAAAG ACCTTACATC TTTGCTCATT 
CTGGATTTGT CTTTCAACTC TTTGAAATAC CTCCCCACTG ATGTATTCCC CAAAACTCTT 
CAAGTACTTC ATCTTGACTA TAATTCGATT TATTCAGTAG ATCCAAATCT CTTCAGCACC 
CTCAGCTACC TCAGCCTGAT GAATAACGAT TTCCGATGTG ATTGTGACTT GAAGGATTTC 
CAAACATGGC TAAATCAAAC CAACATAACC TTCGTTCACT CTATTGAGGA TGTGACGTGT 
GCCAGTCCTG AGGATCAGTA CATGGTTCCG GTTGTGAGTT CCAGCATACA ATGTGAGGAT 
GAAGAGGAGG AGAGAAGAAC TGAAAAACTA AGACTTGTGC TTTTTATTTC CTGCACTGTA 
TTGATTATAT TATTCAGCGC CAGCACCATT GTTTACATCA GTCGACGCGG TGTCATCTTC 
AAGATATACA AAAAACTAAT TGGCAAATTT GACAATAAAC CTCAAGAGGA ACCTGATGCA 
AATGGATTTT TGTACGATGT GTACCTGTGT TTCAGTTCCA GAGACATGAA GTGGGTAGAA 
AGAGCGTTGT TGAAGAGGCT GGACTCTCAG TTTTCAGAGC ACAACACACT CCGCTGCTGC 
TTCGAGGAGC GAGACTTCAT CCCCGGGGAG GACCATCTTA CCAATATGAG AAATGCCATC 
CAGCATAGTC GAAAAACCAT TTGCGTGGTG TCTAAACACT TTCTGAAGGA CGGCTGGTGT 
CTAGAAACAT TCACCCTGGC ACAATGTAGG ATGCTAGTGG AGCTTAAGGA CATTCTGGTG 
GTGCTGGTTG TAGGAAACAT ACCGCAGTAC AGGTTATTAA AGTATGAACA ACTGAGATCC 
TTCATTGAGA ACAGAAGTTA CCTCGTGTGG CCTGATGACG GTCAAGACTT GGAGTGGTTT 
TATGACCAAC TTCTGCACAA GATAATCAGA AACACCAAGG TTAAACAAAC AAACATTAAA 
GAAAAAGACA AGGTGGATGA AAACAATCCT GAAGCAGCAG ATGTTCAAGC AGACACTGCA 
GTTTAA