s1pr4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	s1pr4
Entrez Gene ID	402857
Full Name	sphingosine-1-phosphate receptor 4
Synonyms	edg6,im:6805837,si:ch211-35n24.1
General protein information	Preferred Names sphingosine-1-phosphate receptor 4 Names sphingosine 1-phosphate receptor 4 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001118891.1	NP_001112363.1	sphingosine 1-phosphate receptor 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	S1PR4	XP_512266.2
Bos taurus (cattle)	S1PR4	XP_005209070.1
Xenopus tropicalis (tropical clawed frog)	s1pr4	XP_002936376.1
Canis lupus familiaris (dog)	S1PR4	XP_854902.1
	S1PR4	XP_001100113.1
Mus musculus (house mouse)	S1pr4	NP_034232.1
Homo sapiens (human)	S1PR4	NP_003766.1
Rattus norvegicus (Norway rat)	S1pr4	NP_001101545.1
Gallus gallus (chicken)	S1PR4	XP_004948783.1
Danio rerio (zebrafish)	s1pr4	NP_001112363.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

Meta-analysis of rare and common exome chip variants identifies S1PR4 and other loci influencing blood cell traits.

Nature genetics48(8)867-76(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A nonclassical MHC class I U lineage locus in zebrafish with a null haplotypic variant.
Dirscherl H, Yoder JA
Immunogenetics67(9)501-13(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following s1pr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the s1pr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31348	NM_001118891.1 Latest version!	Danio rerio sphingosine-1-phosphate receptor 4 (s1pr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

s1pr4 ( NM_001118891.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001112363.1 Danio rerio sphingosine-1-phosphate receptor 4 (s1pr4), mRNA.

Price & Availability↑

CloneID	ODa31348
Clone ID Related Accession (Same CDS sequence)	NM_001118891.1
Accession Version	NM_001118891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	sphingosine 1-phosphate receptor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU855875.13. On Mar 7, 2008 this sequence version replaced XM_001334806.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01034324.1, GDQH01019372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA4476734 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGTCC TGCGTTCCTT CAGCTCCGCC TCATCCTGTC CCGACGTCTA CAACTGGACC 
AGCAACAGCC TCATCCTGGA GCACTACAAC TACACGGGAC GCCTCCACCA CCGCAAGCCC 
TCAAAGAAAA GCGTGAGCGC TGTCACCGTC CTGTTCCTGT GCTTCAGCGT CTTCATCATC 
CTGGAGAACC TCATGGTGCT CCTAGCCGTG CTGTTTCGGG TGCGTCTGCG CCACCGTTGG 
ATATTCATCT GCATTGCCAA CATTGCACTG AGTGACTTGT TGACGGGAAC ATCATACGTG 
GTCAACATCT GCATGTCCGG CGAACAAACC TTCAGGCTAA CCCCAATTCT CTGGCTATTC 
CGAGAAGGAC TTTTATTTGT AGCTTTAGCG GCATCCATCT TTAGCCTTCT CTTGATCGCT 
GTAGAGCGAT ATGCGACTAT GATGAAACCC GTCCACCAGA AAACCGCAAC AAAGACCTAC 
CGAGTCTACA TCATGATGCT GTTATGCTGG ATAACGGCGT TTATAATCGG ATTCCTTCCA 
CTTATGGGCT GGAACTGCGT TTGCGATCTG AGAAGTTGCT CGACACTTCT TCCGCTCTAC 
TCCAAGAGCT ACATCTTGTT CGCATTGGTC ATCTTCTTCA TAATTTTGCT CACAATCGGC 
GTACTCTATT TTGCAATCTA TTGCCATGTA CGAAGTAGCA CTGAAACGAT ATCCGCACGC 
AGTCGCAAGC GTTCGCTACG CCTCCTGAAA GCCGTCATTT CAATTGTTGG GGTCTTCATG 
GTATGTTGGG GTCCACTGTT CATCCTCTTA TTAGTGGATT TCTTTTGTTA CTCTCGAAGT 
TGCAGCCCTC TTTTCAATCC CGAATGGGTC ATTGCGCTAG CCGTACTCAA CTCTGCCATG 
AATCCGCTCA TTTATTCATT CGGCAGTCTG GAGCTTCGCA AAGCCATCGC GACGCTACTG 
TGCTGCAGTT GTCTGAAGGC AGGATTGTGC GATCCCAAAA CCTTCATGTC CAAGGAGACC 
TCCAGCACCT CAGGGAGTCG ACACAGCAGC TTGCGCAACA GCTTCAGCAA GGTGCGAAAC 
CTGAGCACGA GTCCTCCGCC CGAACCGAAG AACGGCAAAA AAACACGCCT CAGCTCCACC 
ACTTCTTGTT TATCTGCGTC AAGCGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001112363.1
CDS	184..1353
Translation	MEVLRSFSSASSCPDVYNWTSNSLILEHYNYTGRLHHRKPSKKSVSAVTVLFLCFSVFIILENLMVLLAVLFRVRLRHRWIFICIANIALSDLLTGTSYVVNICMSGEQTFRLTPILWLFREGLLFVALAASIFSLLLIAVERYATMMKPVHQKTATKTYRVYIMMLLCWITAFIIGFLPLMGWNCVCDLRSCSTLLPLYSKSYILFALVIFFIILLTIGVLYFAIYCHVRSSTETISARSRKRSLRLLKAVISIVGVFMVCWGPLFILLLVDFFCYSRSCSPLFNPEWVIALAVLNSAMNPLIYSFGSLELRKAIATLLCCSCLKAGLCDPKTFMSKETSSTSGSRHSSLRNSFSKVRNLSTSPPPEPKNGKKTRLSSTTSCLSASSG

Target ORF information:

RefSeq Version	NM_001118891.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sphingosine-1-phosphate receptor 4 (s1pr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001118891.1

ORF Insert Sequence:

ATGGAAGTCC TGCGTTCCTT CAGCTCCGCC TCATCCTGTC CCGACGTCTA CAACTGGACC 
AGCAACAGCC TCATCCTGGA GCACTACAAC TACACGGGAC GCCTCCACCA CCGCAAGCCC 
TCAAAGAAAA GCGTGAGCGC TGTCACCGTC CTGTTCCTGT GCTTCAGCGT CTTCATCATC 
CTGGAGAACC TCATGGTGCT CCTAGCCGTG CTGTTTCGGG TGCGTCTGCG CCACCGTTGG 
ATATTCATCT GCATTGCCAA CATTGCACTG AGTGACTTGT TGACGGGAAC ATCATACGTG 
GTCAACATCT GCATGTCCGG CGAACAAACC TTCAGGCTAA CCCCAATTCT CTGGCTATTC 
CGAGAAGGAC TTTTATTTGT AGCTTTAGCG GCATCCATCT TTAGCCTTCT CTTGATCGCT 
GTAGAGCGAT ATGCGACTAT GATGAAACCC GTCCACCAGA AAACCGCAAC AAAGACCTAC 
CGAGTCTACA TCATGATGCT GTTATGCTGG ATAACGGCGT TTATAATCGG ATTCCTTCCA 
CTTATGGGCT GGAACTGCGT TTGCGATCTG AGAAGTTGCT CGACACTTCT TCCGCTCTAC 
TCCAAGAGCT ACATCTTGTT CGCATTGGTC ATCTTCTTCA TAATTTTGCT CACAATCGGC 
GTACTCTATT TTGCAATCTA TTGCCATGTA CGAAGTAGCA CTGAAACGAT ATCCGCACGC 
AGTCGCAAGC GTTCGCTACG CCTCCTGAAA GCCGTCATTT CAATTGTTGG GGTCTTCATG 
GTATGTTGGG GTCCACTGTT CATCCTCTTA TTAGTGGATT TCTTTTGTTA CTCTCGAAGT 
TGCAGCCCTC TTTTCAATCC CGAATGGGTC ATTGCGCTAG CCGTACTCAA CTCTGCCATG 
AATCCGCTCA TTTATTCATT CGGCAGTCTG GAGCTTCGCA AAGCCATCGC GACGCTACTG 
TGCTGCAGTT GTCTGAAGGC AGGATTGTGC GATCCCAAAA CCTTCATGTC CAAGGAGACC 
TCCAGCACCT CAGGGAGTCG ACACAGCAGC TTGCGCAACA GCTTCAGCAA GGTGCGAAAC 
CTGAGCACGA GTCCTCCGCC CGAACCGAAG AACGGCAAAA AAACACGCCT CAGCTCCACC 
ACTTCTTGTT TATCTGCGTC AAGCGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

Meta-analysis of rare and common exome chip variants identifies S1PR4 and other loci influencing blood cell traits.
Nature genetics48(8)867-76(2016 08)

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A nonclassical MHC class I U lineage locus in zebrafish with a null haplotypic variant.
Immunogenetics67(9)501-13(2015 Sep)
Dirscherl H,Yoder JA