tnni2a.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnni2a.2
Entrez Gene ID	394068
Full Name	troponin I type 2a (skeletal, fast), tandem duplicate 2
Synonyms	Tnni2,zgc:56294
General protein information	Preferred Names troponin I type 2a (skeletal, fast), tandem duplicate 2 Names troponin tropI-1b troponin I, skeletal, fast 2a, tandem duplicate 2 troponin I, skeletal, fast 2a.2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201093.1	NP_957387.1	troponin

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnni2	NP_033431.1
Rattus norvegicus (Norway rat)	Tnni2	NP_058881.1
Danio rerio (zebrafish)	tnni2a.1	NP_001007366.1
Danio rerio (zebrafish)	tnni2a.4	NP_001009901.2
Pan troglodytes (chimpanzee)	TNNI2	XP_001153267.1
Bos taurus (cattle)	TNNI2	NP_001179023.1
Xenopus tropicalis (tropical clawed frog)	tnni2	NP_001011110.1
	LOC721041	XP_001117040.1
Canis lupus familiaris (dog)	TNNI2	XP_851068.1
Danio rerio (zebrafish)	tnni2a.3	NP_991138.2
Danio rerio (zebrafish)	tnni2b.1	NP_001129964.1
Danio rerio (zebrafish)	tnni2a.2	NP_957387.1
Homo sapiens (human)	TNNI2	NP_001139301.1
Gallus gallus (chicken)	TNNI2	NP_990748.1
Danio rerio (zebrafish)	tnni2b.2	NP_001003423.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC.
Genge CE, Stevens CM, Davidson WS, Singh G, Peter Tieleman D, Tibbits GF
Genome biology and evolution8(4)994-1011(2016 Apr)

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Shih YH, Zhang Y, Ding Y, Ross CA, Li H, Olson TM, Xu X
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)

An update on MyoD evolution in teleosts and a proposed consensus nomenclature to accommodate the tetraploidization of different vertebrate genomes.
Macqueen DJ, Johnston IA
PloS one3(2)e1567(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnni2a.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnni2a.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02587	NM_201093.1 Latest version!	Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 2 (tnni2a.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnni2a.2 ( NM_201093.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957387.1 Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 2 (tnni2a.2), mRNA.

Price & Availability↑

CloneID	ODa02587
Clone ID Related Accession (Same CDS sequence)	NM_201093.1
Accession Version	NM_201093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	troponin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC047857.1. ##Evidence-Data-START## Transcript exon combination :: BC047857.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END##

ATGCTCCAGG TTGCAAAAGA TTTACTGGAA GCTGAGGCTG TTGAGAAGAA GGAGGAGAGG 
AAGAAATACA TGGAAGAACA TTGTCCTGCA TTTTCATGCC CAAGCTCCAA ACAGGAACTA 
GAGGAGTTCT GCAGAAAATT ACATGAAAAA ATTGAAGTGA TTGATGAGGA AAGATATGTT 
CTCGAGCACA AAGCCACTAT GGTTGTCAAT GAGGTCAAAG ACTTAAACAT CAAGATCGTC 
GACCTAAAGG GCAAGTTCAA GAAGCCTCCT TTGAAGAAAG TGCGCATGTC TGCTGACGCT 
ATGCTTCAGG CTCTGCTGGG CTCCAAACAC AAGGTCTCCA TGGATCTGAG GGCCAACCTC 
AAACAAGTCA AGAAGGAGGT CAAAGAGGAG AATAAAGATT TGCGTGATGT TGGTGACTGG 
CGAAAGAATA TTGAGGATAA AACTGGCATG GGCGGCAGGA AGAAGATGTT TGAATCTGAA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957387.1
CDS	172..657
Translation	MLQVAKDLLEAEAVEKKEERKKYMEEHCPAFSCPSSKQELEEFCRKLHEKIEVIDEERYVLEHKATMVVNEVKDLNIKIVDLKGKFKKPPLKKVRMSADAMLQALLGSKHKVSMDLRANLKQVKKEVKEENKDLRDVGDWRKNIEDKTGMGGRKKMFESEA

Target ORF information:

RefSeq Version	NM_201093.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 2 (tnni2a.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201093.1

ORF Insert Sequence:

ATGCTCCAGG TTGCAAAAGA TTTACTGGAA GCTGAGGCTG TTGAGAAGAA GGAGGAGAGG 
AAGAAATACA TGGAAGAACA TTGTCCTGCA TTTTCATGCC CAAGCTCCAA ACAGGAACTA 
GAGGAGTTCT GCAGAAAATT ACATGAAAAA ATTGAAGTGA TTGATGAGGA AAGATATGTT 
CTCGAGCACA AAGCCACTAT GGTTGTCAAT GAGGTCAAAG ACTTAAACAT CAAGATCGTC 
GACCTAAAGG GCAAGTTCAA GAAGCCTCCT TTGAAGAAAG TGCGCATGTC TGCTGACGCT 
ATGCTTCAGG CTCTGCTGGG CTCCAAACAC AAGGTCTCCA TGGATCTGAG GGCCAACCTC 
AAACAAGTCA AGAAGGAGGT CAAAGAGGAG AATAAAGATT TGCGTGATGT TGGTGACTGG 
CGAAAGAATA TTGAGGATAA AACTGGCATG GGCGGCAGGA AGAAGATGTT TGAATCTGAA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC.
Genome biology and evolution8(4)994-1011(2016 Apr)
Genge CE,Stevens CM,Davidson WS,Singh G,Peter Tieleman D,Tibbits GF

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)
Shih YH,Zhang Y,Ding Y,Ross CA,Li H,Olson TM,Xu X

An update on MyoD evolution in teleosts and a proposed consensus nomenclature to accommodate the tetraploidization of different vertebrate genomes.
PloS one3(2)e1567(2008 Feb)
Macqueen DJ,Johnston IA