mad1l1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mad1l1
Entrez Gene ID	393886
Full Name	mitotic arrest deficient 1 like 1
Synonyms	zgc:56571
General protein information	Preferred Names mitotic arrest deficient 1 like 1 Names mitotic spindle assembly checkpoint protein MAD1 MAD1 mitotic arrest deficient-like 1 mitotic arrest deficient-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200912.2	NP_957206.2	mitotic spindle assembly checkpoint protein MAD1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAD1L1	XP_518933.2
Bos taurus (cattle)	MAD1L1	NP_001095514.1
Gallus gallus (chicken)	MAD1L1	XP_004945193.1
	MAD1L1	XP_001102100.1
Canis lupus familiaris (dog)	MAD1L1	XP_852066.2
Mus musculus (house mouse)	Mad1l1	NP_034882.2
Drosophila melanogaster (fruit fly)	Mad1	NP_610477.2
Homo sapiens (human)	MAD1L1	NP_001013858.1
Rattus norvegicus (Norway rat)	Mad1l1	NP_001102857.1
Danio rerio (zebrafish)	mad1l1	NP_957206.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006632	XP_316663.4
Xenopus tropicalis (tropical clawed frog)	mad1l1	NP_001006927.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
Liu Z, Lin X, Cai Z, Zhang Z, Han C, Jia S, Meng A, Wang Q
The Journal of biological chemistry286(32)28520-32(2011 Aug)

Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos.
Jeong K, Jeong JY, Lee HO, Choi E, Lee H
Developmental biology345(1)34-48(2010 Sep)

Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio).
Miranda CL, Collodi P, Zhao X, Barnes DW, Buhler DR
Archives of biochemistry and biophysics305(2)320-7(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mad1l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mad1l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36029	NM_200912.2 Latest version!	Danio rerio mitotic arrest deficient 1 like 1 (mad1l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mad1l1 ( NM_200912.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957206.2 Danio rerio mitotic arrest deficient 1 like 1 (mad1l1), mRNA.

Price & Availability↑

CloneID	ODa36029
Clone ID Related Accession (Same CDS sequence)	NM_200912.2
Accession Version	NM_200912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mitotic spindle assembly checkpoint protein MAD1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX927386.11 and BX649473.9. On Jul 17, 2010 this sequence version replaced NM_200912.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01019544.1, GFIL01025728.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACGATA TAGAGGATGA CACCACAATC TTCTCAACGC TTAAATCTTT CAAGAGTTTA 
ATATCATGCC CAGAACGTTC CTTGCTGGAA ACTGAGCCAG CCTATGGAAG AAGTGACCTT 
CAGAAGCTCT ATACTAAAAG AGTGGAGTTG GAGGAGGCTG CCGAGCGCGT TCGTTCCCAC 
ACCAGCCTGA TTCAGCTCAC TCAAGAGAAA CAACAGATGG AGCTGAGTCA CAAACGAGCC 
CGCATAGAGC TGGAGAAAGA GGCGCACAGC AGCTCCAGAG ACTTACAGAG GGAAATTGAC 
CGTAACAGGG ACTTGCAGAT GAAGATCAGA CGTCTGGAAG AAAGAGAAGA GAAAGCAAAT 
CAAGCCCTTA ATGAACAAAT GGAAAACAAC AAAGCTTTAA AGCGCAACCT CGAGGAACTC 
CACAAGAAAG CAAATGAAAA GGACAGCAAA CTTACCGAGG CAGAGCAGAT TATCAGTGAG 
TTGAAAGATG AGACGAGAAG GCTCGGTCAG CAGATCCAGA TTCAGGAGTC TAAATTCTCC 
ACCCAGAACC TGGAGATCCA GGCCTTGCAG GAACAACTGG ATGTGCAGCG CAAGAAGTTT 
CAGGACATCA GTCAGAAGTA CCAAAATCTG CAGTCCTCTC ATTCAACCAG CACAGAGAGC 
GAACTCAAGA TGAAGGAGTT GGAGAGGCGT TTGGCTCTTC AGGAACAGGA CTCCGTGATT 
GTGAAGAACA TGAAATCTGA AGTGGCCAGA CTGCCTGAAA TGGAGCGGGA AATTAAACGT 
CTGCGTGAGG AAAACTCTTT CTTAAGGGAG ACAAAAGAGA ACAACAGTTT GCTGAAGGAA 
GAGACCGAGG GCTTGAGGAG GAAGCTGGAG AGGCTGGAGA AAGTGATGGA GGAGAAAATC 
AAAGTGGAGT TGGAGAAAGA GAAAATGGAA CGCGAGCTGC AGGCTTGGGA AAACATTGGC 
CAGGCTACAG GACTTAATAT TAGGAAACCT GAGGATTTAT CCAGGGAGGT GATACAGATT 
CAGCAGCGAG AACTGAACTT CAAACAGCAG AACTATAGCC TCAATAGCAG TGTTCGCTCT 
GTGGAAAAGG CCCGCTCTGA GCTCCTGACC GAGATTGCTC AGCTGCGCGC CAAGGCTCAA 
GAAGAACAGA AGAAACGAGA GAACCAGGAC TCACTGGTCC GTCGCTTGCA GAAGAGAGTC 
TTACTGCTCA CCAAGGAGCG CGATGGAATG AGGGCCATAT TAGAGTCGTA CGACAGCGAG 
CTGGCCACAA GTGAATACTC TCCTCAGCTC ACACTCCGGG TCAAGGAGGC AGAGGACATG 
CTGCAGAAAG TCCAGGCTCA TAATGCAGAG ATGGAGACTC AACTGTCCAA AGCCCAGGAG 
GAGGCTGGCT CCTTCAAATT ACAAGCTCAG ATGGTGGCTG CTGAACTTGA GGCTCTGAAA 
GAGCAGCAGG TGTCTAATGC CGAGAGAAGC AGCTCAGTGA CAGCAGAGGA AATCAGCTCT 
CTCAGGCAGA AGATTGAGGA GTTGGAAGCA GAACGTCAGC GTTTGGAAGA GCAAAATAAC 
ATTCTGGAGA TGAGACTAGA GAGGCATAAC CTCCAGGGAG ACTACGACCC TGTTAAGACG 
AAGGTGGTCC ACCTGCAGAT GAACCCCACC AGCATGGCAA AGCAGCAGCG GGCGGATGAG 
GTGGAGCAGC TCAGGGTGGA GTGTCAGCGT TTGAGGGACC GACTCCGCAA AATCGAAGTG 
GCTGGTGGCA TGACAACAGA TGACACTACC CTCATCATCC CACCGTCACA GGAAATACTG 
GACCTGCGTA AGCAAATGGA AAGTGCAGAA CTGAAGAACC AGCGCTTGAA GGAGGTGTTT 
CAGAAGAAGA TTCAAGAGTT TCGTACTGCA TGCTACGTCC TGACTGGGTA CCAGATCGAC 
ATCACTGTGG AGAACCAGTA CCGGCTCACT TCCGTCTACG CCGAGCATAT GGAGGATTCA 
TTACTGTTTA AGTCTACTGG TCCAGTGGGT AGCGGCAGCA TGCAGCTGTT GGAGACGGAT 
TTCTCCCGCA CTCTAACTGG ACTGGTAGAT CTGCACCTCT TCCATCAGAA GAGCATCCCC 
GTGTTCCTCA GCGCGGTCAC CATCGAGCTG TTCAGCCGCC AGACTGTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957206.2
CDS	107..2260
Translation	MDDIEDDTTIFSTLKSFKSLISCPERSLLETEPAYGRSDLQKLYTKRVELEEAAERVRSHTSLIQLTQEKQQMELSHKRARIELEKEAHSSSRDLQREIDRNRDLQMKIRRLEEREEKANQALNEQMENNKALKRNLEELHKKANEKDSKLTEAEQIISELKDETRRLGQQIQIQESKFSTQNLEIQALQEQLDVQRKKFQDISQKYQNLQSSHSTSTESELKMKELERRLALQEQDSVIVKNMKSEVARLPEMEREIKRLREENSFLRETKENNSLLKEETEGLRRKLERLEKVMEEKIKVELEKEKMERELQAWENIGQATGLNIRKPEDLSREVIQIQQRELNFKQQNYSLNSSVRSVEKARSELLTEIAQLRAKAQEEQKKRENQDSLVRRLQKRVLLLTKERDGMRAILESYDSELATSEYSPQLTLRVKEAEDMLQKVQAHNAEMETQLSKAQEEAGSFKLQAQMVAAELEALKEQQVSNAERSSSVTAEEISSLRQKIEELEAERQRLEEQNNILEMRLERHNLQGDYDPVKTKVVHLQMNPTSMAKQQRADEVEQLRVECQRLRDRLRKIEVAGGMTTDDTTLIIPPSQEILDLRKQMESAELKNQRLKEVFQKKIQEFRTACYVLTGYQIDITVENQYRLTSVYAEHMEDSLLFKSTGPVGSGSMQLLETDFSRTLTGLVDLHLFHQKSIPVFLSAVTIELFSRQTVA

Target ORF information:

RefSeq Version	NM_200912.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitotic arrest deficient 1 like 1 (mad1l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200912.2

ORF Insert Sequence:

ATGGACGATA TAGAGGATGA CACCACAATC TTCTCAACGC TTAAATCTTT CAAGAGTTTA 
ATATCATGCC CAGAACGTTC CTTGCTGGAA ACTGAGCCAG CCTATGGAAG AAGTGACCTT 
CAGAAGCTCT ATACTAAAAG AGTGGAGTTG GAGGAGGCTG CCGAGCGCGT TCGTTCCCAC 
ACCAGCCTGA TTCAGCTCAC TCAAGAGAAA CAACAGATGG AGCTGAGTCA CAAACGAGCC 
CGCATAGAGC TGGAGAAAGA GGCGCACAGC AGCTCCAGAG ACTTACAGAG GGAAATTGAC 
CGTAACAGGG ACTTGCAGAT GAAGATCAGA CGTCTGGAAG AAAGAGAAGA GAAAGCAAAT 
CAAGCCCTTA ATGAACAAAT GGAAAACAAC AAAGCTTTAA AGCGCAACCT CGAGGAACTC 
CACAAGAAAG CAAATGAAAA GGACAGCAAA CTTACCGAGG CAGAGCAGAT TATCAGTGAG 
TTGAAAGATG AGACGAGAAG GCTCGGTCAG CAGATCCAGA TTCAGGAGTC TAAATTCTCC 
ACCCAGAACC TGGAGATCCA GGCCTTGCAG GAACAACTGG ATGTGCAGCG CAAGAAGTTT 
CAGGACATCA GTCAGAAGTA CCAAAATCTG CAGTCCTCTC ATTCAACCAG CACAGAGAGC 
GAACTCAAGA TGAAGGAGTT GGAGAGGCGT TTGGCTCTTC AGGAACAGGA CTCCGTGATT 
GTGAAGAACA TGAAATCTGA AGTGGCCAGA CTGCCTGAAA TGGAGCGGGA AATTAAACGT 
CTGCGTGAGG AAAACTCTTT CTTAAGGGAG ACAAAAGAGA ACAACAGTTT GCTGAAGGAA 
GAGACCGAGG GCTTGAGGAG GAAGCTGGAG AGGCTGGAGA AAGTGATGGA GGAGAAAATC 
AAAGTGGAGT TGGAGAAAGA GAAAATGGAA CGCGAGCTGC AGGCTTGGGA AAACATTGGC 
CAGGCTACAG GACTTAATAT TAGGAAACCT GAGGATTTAT CCAGGGAGGT GATACAGATT 
CAGCAGCGAG AACTGAACTT CAAACAGCAG AACTATAGCC TCAATAGCAG TGTTCGCTCT 
GTGGAAAAGG CCCGCTCTGA GCTCCTGACC GAGATTGCTC AGCTGCGCGC CAAGGCTCAA 
GAAGAACAGA AGAAACGAGA GAACCAGGAC TCACTGGTCC GTCGCTTGCA GAAGAGAGTC 
TTACTGCTCA CCAAGGAGCG CGATGGAATG AGGGCCATAT TAGAGTCGTA CGACAGCGAG 
CTGGCCACAA GTGAATACTC TCCTCAGCTC ACACTCCGGG TCAAGGAGGC AGAGGACATG 
CTGCAGAAAG TCCAGGCTCA TAATGCAGAG ATGGAGACTC AACTGTCCAA AGCCCAGGAG 
GAGGCTGGCT CCTTCAAATT ACAAGCTCAG ATGGTGGCTG CTGAACTTGA GGCTCTGAAA 
GAGCAGCAGG TGTCTAATGC CGAGAGAAGC AGCTCAGTGA CAGCAGAGGA AATCAGCTCT 
CTCAGGCAGA AGATTGAGGA GTTGGAAGCA GAACGTCAGC GTTTGGAAGA GCAAAATAAC 
ATTCTGGAGA TGAGACTAGA GAGGCATAAC CTCCAGGGAG ACTACGACCC TGTTAAGACG 
AAGGTGGTCC ACCTGCAGAT GAACCCCACC AGCATGGCAA AGCAGCAGCG GGCGGATGAG 
GTGGAGCAGC TCAGGGTGGA GTGTCAGCGT TTGAGGGACC GACTCCGCAA AATCGAAGTG 
GCTGGTGGCA TGACAACAGA TGACACTACC CTCATCATCC CACCGTCACA GGAAATACTG 
GACCTGCGTA AGCAAATGGA AAGTGCAGAA CTGAAGAACC AGCGCTTGAA GGAGGTGTTT 
CAGAAGAAGA TTCAAGAGTT TCGTACTGCA TGCTACGTCC TGACTGGGTA CCAGATCGAC 
ATCACTGTGG AGAACCAGTA CCGGCTCACT TCCGTCTACG CCGAGCATAT GGAGGATTCA 
TTACTGTTTA AGTCTACTGG TCCAGTGGGT AGCGGCAGCA TGCAGCTGTT GGAGACGGAT 
TTCTCCCGCA CTCTAACTGG ACTGGTAGAT CTGCACCTCT TCCATCAGAA GAGCATCCCC 
GTGTTCCTCA GCGCGGTCAC CATCGAGCTG TTCAGCCGCC AGACTGTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
The Journal of biological chemistry286(32)28520-32(2011 Aug)
Liu Z,Lin X,Cai Z,Zhang Z,Han C,Jia S,Meng A,Wang Q

Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos.
Developmental biology345(1)34-48(2010 Sep)
Jeong K,Jeong JY,Lee HO,Choi E,Lee H

Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio).
Archives of biochemistry and biophysics305(2)320-7(1993 Sep)
Miranda CL,Collodi P,Zhao X,Barnes DW,Buhler DR