ndufb8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ndufb8
Entrez Gene ID	393829
Full Name	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8
Synonyms	nduf8,zgc:77718
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200855.1	NP_957149.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Pathways from BioSystems

Homologies

	NDUFB8	XP_001108340.1
Bos taurus (cattle)	NDUFB8	NP_777092.1
Rattus norvegicus (Norway rat)	Ndufb8	NP_001099830.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002020	XP_321035.4
Pan troglodytes (chimpanzee)	NDUFB8	NP_001138336.1
Mus musculus (house mouse)	Ndufb8	NP_080337.1
Homo sapiens (human)	NDUFB8	NP_004995.1
Danio rerio (zebrafish)	ndufb8	NP_957149.1
Drosophila melanogaster (fruit fly)	CG3192	NP_001027048.1
Xenopus tropicalis (tropical clawed frog)	ndufb8	XP_002936399.2
Canis lupus familiaris (dog)	NDUFB8	XP_005637678.1
Gallus gallus (chicken)	NDUFB8	NP_001006502.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Opa1 is required for proper mitochondrial metabolism in early development.
Rahn JJ, Stackley KD, Chan SS
PloS one8(3)e59218(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ndufb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ndufb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37215	NM_200855.1 Latest version!	Danio rerio NADH:ubiquinone oxidoreductase subunit B8 (ndufb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ndufb8 ( NM_200855.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957149.1 Danio rerio NADH:ubiquinone oxidoreductase subunit B8 (ndufb8), mRNA.

Price & Availability↑

CloneID	ODa37215
Clone ID Related Accession (Same CDS sequence)	NM_200855.1
Accession Version	NM_200855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062842.1. ##Evidence-Data-START## Transcript exon combination :: BC062842.1, EH505049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGT TCAGAGCGGG TCTCCTGACC CCAGCTCTAA CTAAAGGAAA ATCTTCTTCA 
ATCTCTGCTA TTATCCGCGG AGCGAGAGCA GCTTCTGGTT CTTCTAAGGA CAGTCTGCCC 
GGTCCGTACC CCAAAACCGC AGAGGAGAAA GCAGCAGCGG CCAAGAAATA CAACATGAGG 
CCTGAGGATT ATCAGCCCTA TCCTGATGAT GGCTGGGGGA GTGGCGATTA CCCCATGCTG 
CCCAGCCGAT CACAGCACGA GAGAGACCCC TATTATCAGT GGGACCATCC AGATCTCAGG 
AGAAACTGGG GAGAACCGAT GCATTATCAC TTCGACATGT ACATCAGGAA CCGGGTGGAC 
ACGTCTCCCA CACCCCTGGA CTGGCGCACC ATGAGACTGT GGCTGTTTGG GTTTCTGGGT 
CTCACCATGT TCATGTTTGG GATGGGGGAG GTTTTACCCT CCTACCAACC CGTGGCAGCC 
AAACAGTATC CCTACAACAA CCTGTATCTG GAGAGAGGAG GAGATCCAGA GAAAGAGCCT 
GAGCCTGTCA AAAACTACGA GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957149.1
CDS	11..577
Translation	MAAFRAGLLTPALTKGKSSSISAIIRGARAASGSSKDSLPGPYPKTAEEKAAAAKKYNMRPEDYQPYPDDGWGSGDYPMLPSRSQHERDPYYQWDHPDLRRNWGEPMHYHFDMYIRNRVDTSPTPLDWRTMRLWLFGFLGLTMFMFGMGEVLPSYQPVAAKQYPYNNLYLERGGDPEKEPEPVKNYEI

Target ORF information:

RefSeq Version	NM_200855.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NADH:ubiquinone oxidoreductase subunit B8 (ndufb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200855.1

ORF Insert Sequence:

ATGGCGGCGT TCAGAGCGGG TCTCCTGACC CCAGCTCTAA CTAAAGGAAA ATCTTCTTCA 
ATCTCTGCTA TTATCCGCGG AGCGAGAGCA GCTTCTGGTT CTTCTAAGGA CAGTCTGCCC 
GGTCCGTACC CCAAAACCGC AGAGGAGAAA GCAGCAGCGG CCAAGAAATA CAACATGAGG 
CCTGAGGATT ATCAGCCCTA TCCTGATGAT GGCTGGGGGA GTGGCGATTA CCCCATGCTG 
CCCAGCCGAT CACAGCACGA GAGAGACCCC TATTATCAGT GGGACCATCC AGATCTCAGG 
AGAAACTGGG GAGAACCGAT GCATTATCAC TTCGACATGT ACATCAGGAA CCGGGTGGAC 
ACGTCTCCCA CACCCCTGGA CTGGCGCACC ATGAGACTGT GGCTGTTTGG GTTTCTGGGT 
CTCACCATGT TCATGTTTGG GATGGGGGAG GTTTTACCCT CCTACCAACC CGTGGCAGCC 
AAACAGTATC CCTACAACAA CCTGTATCTG GAGAGAGGAG GAGATCCAGA GAAAGAGCCT 
GAGCCTGTCA AAAACTACGA GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Opa1 is required for proper mitochondrial metabolism in early development.
PloS one8(3)e59218(2013)
Rahn JJ,Stackley KD,Chan SS