slc5a12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc5a12
Entrez Gene ID	393339
Full Name	solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12
Synonyms	smctn,zgc:64037
General protein information	Preferred Names solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12 Names sodium-coupled monocarboxylate transporter 2 electroneutral sodium monocarboxylate cotransporter low-affinity sodium-lactate cotransporter solute carrier family 5 (sodium/glucose cotransporter), member 12 solute carrier family 5 member 12 zSLC5A12 zSMCTn
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298195.2	XP_009296470.1	sodium-coupled monocarboxylate transporter 2 isoform X1
NM_200368.1	NP_956662.1	sodium-coupled monocarboxylate transporter 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc5a12	NP_001171095.1
Rattus norvegicus (Norway rat)	Slc5a12	NP_001102058.1
Gallus gallus (chicken)	SLC5A12	XP_428889.1
Danio rerio (zebrafish)	slc5a12	NP_956662.1
	SLC5A12	XP_001090490.2
Canis lupus familiaris (dog)	SLC5A12	XP_534095.3
Bos taurus (cattle)	SLC5A12	NP_001094529.1
Drosophila melanogaster (fruit fly)	CG10444	NP_611465.2
Caenorhabditis elegans (roundworm)	ZK822.5	NP_001041067.1
Xenopus tropicalis (tropical clawed frog)	slc5a12	XP_004913649.1
Homo sapiens (human)	SLC5A12	NP_848593.2
Pan troglodytes (chimpanzee)	SLC5A12	XP_508335.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004199	XP_313095.4

Gene Ontology
Bibliography

Related articles in PubMed

Insulin and SGK1 reduce the function of Na+/monocarboxylate transporter 1 (SMCT1/SLC5A8).
L?pez-Barradas A, Gonz?lez-Cid T, V?zquez N, Gavi-Maza M, Reyes-Camacho A, Vel?zquez-Villegas LA, Ram?rez V, Zandi-Nejad K, Mount DB, Torres N, Tovar AR, Romero MF, Gamba G, Plata C
American journal of physiology. Cell physiology311(5)C720-C734(2016 Nov)

Solute carriers (SLCs) identified and characterized from kidney transcriptome of golden mahseer (Tor putitora) (Fam: Cyprinidae).
Barat A, Sahoo PK, Kumar R, Pande V
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology20054-61(2016 Oct)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish Slc5a12 encodes an electroneutral sodium monocarboxylate transporter (SMCTn). A comparison with the electrogenic SMCT (SMCTe/Slc5a8).
Plata C, Sussman CR, Sindic A, Liang JO, Mount DB, Josephs ZM, Chang MH, Romero MF
The Journal of biological chemistry282(16)11996-2009(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

zSMCTe is the zebrafish SLC5A8 ortholog, and zSMCTn is a novel, electroneutral SMCT (zSlc5a12)
Title: Zebrafish Slc5a12 encodes an electroneutral sodium monocarboxylate transporter (SMCTn). A comparison with the electrogenic SMCT (SMCTe/Slc5a8).

The following slc5a12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc5a12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03194	NM_200368.1 Latest version!	Danio rerio solute carrier family 5 member 12 (slc5a12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40430	XM_009298195.2 Latest version!	Danio rerio solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12 (slc5a12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc5a12 ( NM_200368.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956662.1 Danio rerio solute carrier family 5 member 12 (slc5a12), mRNA.

Price & Availability↑

CloneID	ODa03194
Clone ID Related Accession (Same CDS sequence)	NM_200368.1
Accession Version	NM_200368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	sodium-coupled monocarboxylate transporter 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053215.1. ##Evidence-Data-START## Transcript exon combination :: BC053215.1, AY727860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGACCG TGGGCAGGTT CCAGGCCGGG GATTATGTGG TGTTTGCGTG TCTGTTCGTG 
GTCTCCTCCG GCATCGGCGT CTTCTTCGCC ATCAAGGAGA GAAACAAAGC CCCGTCCAAG 
GAGTTTCTGG TGGGGGGCCG TCAGATGTCC TGCGGGCCCG TGGCTCTGTC CCTCACCGCC 
AGCTTCATGT CTGCTGTGAC TGTGATCGGG GCTCCGGCAG ACGTCTACAG ATTCGGGGCC 
TCGTACGTTA TCTTCGGGGT CGCCTACACG TTTGTGGTGT TTTTTACGGC TGAGCTCTTC 
CTGCCCGTGT TTTACAGGTC AGGAATCACC AGCACATATG AGTACTTGGA GTTGCGTTTC 
TGCAAGCTCG TTCGCGTCGC AGCCACTCTA ATTTACATCA TTCAGACGAT TCTCTACACT 
GGTGTGGTGG TTTATGCTCC TGCTTTAGCT CTCAATCAAG TGACAGGATT TGACCTTTGG 
GGATCTATAT TTGCCACCGG CATTGTCTGC ACCTTTTACT GCACACTGGG TGGGCTGAAG 
GCTGTGGTAT GGACCGATGC CTTCCAGATG GTGGTGATGG TGGTGGGCTT CCTCACTGTG 
CTGATCCAGG GTTCGAGTCG AGCTGGAGGA ATCGAGAATG TCTGGAGCAC CTCTCGCACT 
GGAGGACGAT TGCAGGTGTT TGACTTTGAC GTGAGTCCGC TCAGGAGACA CACGTTCTGG 
ACACTCAGTG TCGGAGGAAC CTTCACCTGG CTGGGGATTT ATGGAGTAAA CCAGTCCACA 
ATTCAGAGAT GCATCTCGTG TAAAACAGAA GGTCACGCAC GCTGGGCTCT GTATCTGAAT 
CTGCTGGGTC TGTGGATTAT TCTGTTCTGC GCTGTGGTTT CTGGCCTGAT CATGTACTCT 
TATTACTCTC ACTGCGATCC CTGGTCATCT GGACTGATCT CTGCGCCAGA TCAGCTCATG 
CCATATTTTG TGATGGAAAT CCTGGGTGCG TTTCCGGGAC TGCCGGGGTT ATTTGTGGCC 
TGTGCCTTCA GCGGCACTCT GAGCACAGTA GCCGCCAGTA TCAATGCTTT GGCCACAGTG 
ATGTACGAGG ACTTTGTGAG CCAGTGTTTC CCAGATCTCT CCAACCGAGC GGCCAGCTGG 
ATCAGCAAAG CGCTCTGTGT GGCGTTTGGT GTGGCCTGCA CTACTATGGC CGTGGCGGCA 
TCATACATGG GAGGAATCGT GCAGGCGGCT CTCAGTATCC ATGGCATGTG CGGAGGGCCG 
GTGCTGGGCC TGTTTTCTCT GGGAATCCTG TTTCCATTCA CTAATCTAAA GGGGGCAGTT 
GGGGGTCTGA TCGTGGGCAT CTCTCTCTCG TTCTGGGTCG GCGTGGGCGC TTTCATTTAC 
CCAGCACCCA GCAATAACAC GCATGCTCTG GAACTCAACA CTGCAGGCTG CAACATCACA 
GCTGCTGCAT TTGAGCCAAC ATCCGCCACA GTGACCCAGC TCACCTCAGA CAGGAATTGG 
CTGGCGGATT CGTGGTACTC CATGTCCTAC CTGTACTACA GTGCAGTGGG CTTTATTGGG 
ACGGTGGCGG CAGGTCTGCT CATCACACTG CTGACAGGCC CAATGGACCC GAAACTGTTG 
AAACCAGGGA TGACTCGATC AGTGAAGGAG GTCATGTGTT TCTGCACTGA GAAATTCACA 
GAAGCTGACC TTGGTGAAGG CAAAGAGGAT GTGGGAGACT TTGGCAAGGC ATGGGAGAAA 
CATCCAGACC AGGGATGCAC CTTAAGAATG GACGAGAAGT TCAGGTGCAG TTGCGATAAC 
CAACAGGAAA ATGGTAACAC TAATGCCGGC TTTGACCATA ACGAAACTAG CATAGTTCAG 
AAAAAGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956662.1
CDS	39..1910
Translation	METVGRFQAGDYVVFACLFVVSSGIGVFFAIKERNKAPSKEFLVGGRQMSCGPVALSLTASFMSAVTVIGAPADVYRFGASYVIFGVAYTFVVFFTAELFLPVFYRSGITSTYEYLELRFCKLVRVAATLIYIIQTILYTGVVVYAPALALNQVTGFDLWGSIFATGIVCTFYCTLGGLKAVVWTDAFQMVVMVVGFLTVLIQGSSRAGGIENVWSTSRTGGRLQVFDFDVSPLRRHTFWTLSVGGTFTWLGIYGVNQSTIQRCISCKTEGHARWALYLNLLGLWIILFCAVVSGLIMYSYYSHCDPWSSGLISAPDQLMPYFVMEILGAFPGLPGLFVACAFSGTLSTVAASINALATVMYEDFVSQCFPDLSNRAASWISKALCVAFGVACTTMAVAASYMGGIVQAALSIHGMCGGPVLGLFSLGILFPFTNLKGAVGGLIVGISLSFWVGVGAFIYPAPSNNTHALELNTAGCNITAAAFEPTSATVTQLTSDRNWLADSWYSMSYLYYSAVGFIGTVAAGLLITLLTGPMDPKLLKPGMTRSVKEVMCFCTEKFTEADLGEGKEDVGDFGKAWEKHPDQGCTLRMDEKFRCSCDNQQENGNTNAGFDHNETSIVQKKL

Target ORF information:

RefSeq Version	NM_200368.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 5 member 12 (slc5a12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200368.1

ORF Insert Sequence:

ATGGAGACCG TGGGCAGGTT CCAGGCCGGG GATTATGTGG TGTTTGCGTG TCTGTTCGTG 
GTCTCCTCCG GCATCGGCGT CTTCTTCGCC ATCAAGGAGA GAAACAAAGC CCCGTCCAAG 
GAGTTTCTGG TGGGGGGCCG TCAGATGTCC TGCGGGCCCG TGGCTCTGTC CCTCACCGCC 
AGCTTCATGT CTGCTGTGAC TGTGATCGGG GCTCCGGCAG ACGTCTACAG ATTCGGGGCC 
TCGTACGTTA TCTTCGGGGT CGCCTACACG TTTGTGGTGT TTTTTACGGC TGAGCTCTTC 
CTGCCCGTGT TTTACAGGTC AGGAATCACC AGCACATATG AGTACTTGGA GTTGCGTTTC 
TGCAAGCTCG TTCGCGTCGC AGCCACTCTA ATTTACATCA TTCAGACGAT TCTCTACACT 
GGTGTGGTGG TTTATGCTCC TGCTTTAGCT CTCAATCAAG TGACAGGATT TGACCTTTGG 
GGATCTATAT TTGCCACCGG CATTGTCTGC ACCTTTTACT GCACACTGGG TGGGCTGAAG 
GCTGTGGTAT GGACCGATGC CTTCCAGATG GTGGTGATGG TGGTGGGCTT CCTCACTGTG 
CTGATCCAGG GTTCGAGTCG AGCTGGAGGA ATCGAGAATG TCTGGAGCAC CTCTCGCACT 
GGAGGACGAT TGCAGGTGTT TGACTTTGAC GTGAGTCCGC TCAGGAGACA CACGTTCTGG 
ACACTCAGTG TCGGAGGAAC CTTCACCTGG CTGGGGATTT ATGGAGTAAA CCAGTCCACA 
ATTCAGAGAT GCATCTCGTG TAAAACAGAA GGTCACGCAC GCTGGGCTCT GTATCTGAAT 
CTGCTGGGTC TGTGGATTAT TCTGTTCTGC GCTGTGGTTT CTGGCCTGAT CATGTACTCT 
TATTACTCTC ACTGCGATCC CTGGTCATCT GGACTGATCT CTGCGCCAGA TCAGCTCATG 
CCATATTTTG TGATGGAAAT CCTGGGTGCG TTTCCGGGAC TGCCGGGGTT ATTTGTGGCC 
TGTGCCTTCA GCGGCACTCT GAGCACAGTA GCCGCCAGTA TCAATGCTTT GGCCACAGTG 
ATGTACGAGG ACTTTGTGAG CCAGTGTTTC CCAGATCTCT CCAACCGAGC GGCCAGCTGG 
ATCAGCAAAG CGCTCTGTGT GGCGTTTGGT GTGGCCTGCA CTACTATGGC CGTGGCGGCA 
TCATACATGG GAGGAATCGT GCAGGCGGCT CTCAGTATCC ATGGCATGTG CGGAGGGCCG 
GTGCTGGGCC TGTTTTCTCT GGGAATCCTG TTTCCATTCA CTAATCTAAA GGGGGCAGTT 
GGGGGTCTGA TCGTGGGCAT CTCTCTCTCG TTCTGGGTCG GCGTGGGCGC TTTCATTTAC 
CCAGCACCCA GCAATAACAC GCATGCTCTG GAACTCAACA CTGCAGGCTG CAACATCACA 
GCTGCTGCAT TTGAGCCAAC ATCCGCCACA GTGACCCAGC TCACCTCAGA CAGGAATTGG 
CTGGCGGATT CGTGGTACTC CATGTCCTAC CTGTACTACA GTGCAGTGGG CTTTATTGGG 
ACGGTGGCGG CAGGTCTGCT CATCACACTG CTGACAGGCC CAATGGACCC GAAACTGTTG 
AAACCAGGGA TGACTCGATC AGTGAAGGAG GTCATGTGTT TCTGCACTGA GAAATTCACA 
GAAGCTGACC TTGGTGAAGG CAAAGAGGAT GTGGGAGACT TTGGCAAGGC ATGGGAGAAA 
CATCCAGACC AGGGATGCAC CTTAAGAATG GACGAGAAGT TCAGGTGCAG TTGCGATAAC 
CAACAGGAAA ATGGTAACAC TAATGCCGGC TTTGACCATA ACGAAACTAG CATAGTTCAG 
AAAAAGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc5a12 ( XM_009298195.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296470.1 Danio rerio solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12 (slc5a12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40430
Clone ID Related Accession (Same CDS sequence)	XM_009298195.2
Accession Version	XM_009298195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sodium-coupled monocarboxylate transporter 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCG TGGGCAGGTT CCAGGCCGGG GATTATGTGG TGTTTGCGTG TCTGTTCGTG 
GTCTCCTCCG GCATCGGCGT CTTCTTCGCC ATCAAGGAGA GAAACAAAGC CCCGTCCAAG 
GAGTTTCTGG TGGGGGGCCG TCAGATGTCC TGCGGGCCCG TGGCTCTGTC CCTCACCGCC 
AGCTTCATGT CTGCTGTGAC TGTGATCGGG GCTCCGGCAG ACGTCTACAG ATTCGGGGCC 
TCGTACGTCA TCTTCGGGGT CGCCTACACG TTTGTGGTGT TTTTTACGGC TGAGCTCTTT 
CTGCCTGTGT TTTACAGGTC AGGAATCACC AGCACATATG AGTATTTGGA GTTGCGTTTC 
TGCAAGCTCG TTCGCGTCGC AGCCACTCTA ATTTACATCA TTCAGACGAT TCTCTACACT 
GGTGTGGTGG TTTATGCTCC TGCTTTAGCT CTCAATCAAG TGACAGGATT TGACCTTTGG 
GGATCTATCT TTGCCACCGG CATTGTCTGC ACTTTTTACT GCACACTGGG TGGGCTGAAG 
GCTGTGGTGT GGACCGATGC CTTCCAGATG GTGGTGATGG TGGTGGGCTT CCTCACTGTG 
CTGATCCAGG GTTCGAGTCG AGCTGGAGGG ATCGAGAATG TCTGGAGCAC CGCTCGCACT 
GGAGGACGAT TGCAGGTGTT TGACTTTGAC GTGAGTCCGC TCAGGAGACA CACGTTCTGG 
ACACTCAGTG TCGGAGGAAC CTTCACCTGG CTGGGGATTT ATGGAGTAAA CCAGTCCACA 
ATTCAGAGAT GCATCTCGTG TAAAACAGAA GGTCACGCAC GCTGGGCTCT GTATCTGAAT 
CTGCTGGGTC TGTGGATTAT TCTGTTCTGC GCTGTGGTTT CTGGCCTGAT CATGTACTCT 
TATTACTCTC ACTGCGATCC CTGGTCATCT GGACTGATCT CTGCGCCAGA TCAGCTCATG 
CCATATTTTG TTATGGAAAT CCTGGGTGCG TTTCCGGGAC TGCCGGGGTT ATTTGTGGCC 
TGTGCCTTCA GCGGCACTCT GAGCACAGTA GCCGCCAGTA TCAATGCTTT GGCCACAGTG 
ATGTACGAGG ACTTTGTGAG CCAGTGTTTC CCAGATCTCT CCAACCGAGC GGCCAGCTGG 
ATCAGCAAAG CGCTCTGTGT GGCGTTTGGT GTGGCCTGCA CTACTATGGC CGTGGCGGCA 
TCATACATGG GAGGAATTGT GCAGGCGGCT CTCAGTATCC ATGGCATGTG CGGAGGGCCG 
GTGCTGGGCC TGTTTTCTCT GGGAATCCTG TTTCCATTCA CTAATCTAAA GGGGGCAGTT 
GGGGGTCTGA TCGTGGGCAT CTCTCTCTCG TTCTGGGTCG GCGTGGGCGC TTTCATTTAC 
CCAGCACCCA GCAATAACAC GCATGCTCTG GAACTCAACA CTGCAGGCTG CAACATCACT 
GCTGCTGCAT TTGAGCCAAC ATCCGCCACA GTGACCCAGC TCACCTCAGA CAGGAATTGG 
CTGGCGGATT CGTGGTACTC CATGTCCTAC CTGTACTACA GTGCAGTGGG CTTTATTGGG 
ACGGTGGCGG CAGGTCTGCT CATCACACTG CTGACAGGCC CAATGGACCC GAAACTGTTG 
AAACCAGGGA TGACTCGATC AGTGAAGGAC GTCCTGTGTT TCTGCACTGA GAAATTCACA 
GAAGCTGACC TTGGTGAAGG CAAAGAGGAT GTGGGAGACT TTGGCAAGGC ATGGGAGAAA 
CATCCAGACC AGGGATGCAC CTTAAGAATG GACGAGAAGT TCAGGTGCAG TTGCGATAAC 
CAACAGGAAA ATGGTAACAC TAATGCCGGC TTTGACCATA ACGAAACTAG CATAGTTCAG 
AAAAAGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296470.1
CDS	291..2162
Translation	METVGRFQAGDYVVFACLFVVSSGIGVFFAIKERNKAPSKEFLVGGRQMSCGPVALSLTASFMSAVTVIGAPADVYRFGASYVIFGVAYTFVVFFTAELFLPVFYRSGITSTYEYLELRFCKLVRVAATLIYIIQTILYTGVVVYAPALALNQVTGFDLWGSIFATGIVCTFYCTLGGLKAVVWTDAFQMVVMVVGFLTVLIQGSSRAGGIENVWSTARTGGRLQVFDFDVSPLRRHTFWTLSVGGTFTWLGIYGVNQSTIQRCISCKTEGHARWALYLNLLGLWIILFCAVVSGLIMYSYYSHCDPWSSGLISAPDQLMPYFVMEILGAFPGLPGLFVACAFSGTLSTVAASINALATVMYEDFVSQCFPDLSNRAASWISKALCVAFGVACTTMAVAASYMGGIVQAALSIHGMCGGPVLGLFSLGILFPFTNLKGAVGGLIVGISLSFWVGVGAFIYPAPSNNTHALELNTAGCNITAAAFEPTSATVTQLTSDRNWLADSWYSMSYLYYSAVGFIGTVAAGLLITLLTGPMDPKLLKPGMTRSVKDVLCFCTEKFTEADLGEGKEDVGDFGKAWEKHPDQGCTLRMDEKFRCSCDNQQENGNTNAGFDHNETSIVQKKL

Target ORF information:

RefSeq Version	XM_009298195.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12 (slc5a12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298195.2

ORF Insert Sequence:

ATGGAGACCG TGGGCAGGTT CCAGGCCGGG GATTATGTGG TGTTTGCGTG TCTGTTCGTG 
GTCTCCTCCG GCATCGGCGT CTTCTTCGCC ATCAAGGAGA GAAACAAAGC CCCGTCCAAG 
GAGTTTCTGG TGGGGGGCCG TCAGATGTCC TGCGGGCCCG TGGCTCTGTC CCTCACCGCC 
AGCTTCATGT CTGCTGTGAC TGTGATCGGG GCTCCGGCAG ACGTCTACAG ATTCGGGGCC 
TCGTACGTCA TCTTCGGGGT CGCCTACACG TTTGTGGTGT TTTTTACGGC TGAGCTCTTT 
CTGCCTGTGT TTTACAGGTC AGGAATCACC AGCACATATG AGTATTTGGA GTTGCGTTTC 
TGCAAGCTCG TTCGCGTCGC AGCCACTCTA ATTTACATCA TTCAGACGAT TCTCTACACT 
GGTGTGGTGG TTTATGCTCC TGCTTTAGCT CTCAATCAAG TGACAGGATT TGACCTTTGG 
GGATCTATCT TTGCCACCGG CATTGTCTGC ACTTTTTACT GCACACTGGG TGGGCTGAAG 
GCTGTGGTGT GGACCGATGC CTTCCAGATG GTGGTGATGG TGGTGGGCTT CCTCACTGTG 
CTGATCCAGG GTTCGAGTCG AGCTGGAGGG ATCGAGAATG TCTGGAGCAC CGCTCGCACT 
GGAGGACGAT TGCAGGTGTT TGACTTTGAC GTGAGTCCGC TCAGGAGACA CACGTTCTGG 
ACACTCAGTG TCGGAGGAAC CTTCACCTGG CTGGGGATTT ATGGAGTAAA CCAGTCCACA 
ATTCAGAGAT GCATCTCGTG TAAAACAGAA GGTCACGCAC GCTGGGCTCT GTATCTGAAT 
CTGCTGGGTC TGTGGATTAT TCTGTTCTGC GCTGTGGTTT CTGGCCTGAT CATGTACTCT 
TATTACTCTC ACTGCGATCC CTGGTCATCT GGACTGATCT CTGCGCCAGA TCAGCTCATG 
CCATATTTTG TTATGGAAAT CCTGGGTGCG TTTCCGGGAC TGCCGGGGTT ATTTGTGGCC 
TGTGCCTTCA GCGGCACTCT GAGCACAGTA GCCGCCAGTA TCAATGCTTT GGCCACAGTG 
ATGTACGAGG ACTTTGTGAG CCAGTGTTTC CCAGATCTCT CCAACCGAGC GGCCAGCTGG 
ATCAGCAAAG CGCTCTGTGT GGCGTTTGGT GTGGCCTGCA CTACTATGGC CGTGGCGGCA 
TCATACATGG GAGGAATTGT GCAGGCGGCT CTCAGTATCC ATGGCATGTG CGGAGGGCCG 
GTGCTGGGCC TGTTTTCTCT GGGAATCCTG TTTCCATTCA CTAATCTAAA GGGGGCAGTT 
GGGGGTCTGA TCGTGGGCAT CTCTCTCTCG TTCTGGGTCG GCGTGGGCGC TTTCATTTAC 
CCAGCACCCA GCAATAACAC GCATGCTCTG GAACTCAACA CTGCAGGCTG CAACATCACT 
GCTGCTGCAT TTGAGCCAAC ATCCGCCACA GTGACCCAGC TCACCTCAGA CAGGAATTGG 
CTGGCGGATT CGTGGTACTC CATGTCCTAC CTGTACTACA GTGCAGTGGG CTTTATTGGG 
ACGGTGGCGG CAGGTCTGCT CATCACACTG CTGACAGGCC CAATGGACCC GAAACTGTTG 
AAACCAGGGA TGACTCGATC AGTGAAGGAC GTCCTGTGTT TCTGCACTGA GAAATTCACA 
GAAGCTGACC TTGGTGAAGG CAAAGAGGAT GTGGGAGACT TTGGCAAGGC ATGGGAGAAA 
CATCCAGACC AGGGATGCAC CTTAAGAATG GACGAGAAGT TCAGGTGCAG TTGCGATAAC 
CAACAGGAAA ATGGTAACAC TAATGCCGGC TTTGACCATA ACGAAACTAG CATAGTTCAG 
AAAAAGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insulin and SGK1 reduce the function of Na+/monocarboxylate transporter 1 (SMCT1/SLC5A8).
American journal of physiology. Cell physiology311(5)C720-C734(2016 Nov)
L?pez-Barradas A,Gonz?lez-Cid T,V?zquez N,Gavi-Maza M,Reyes-Camacho A,Vel?zquez-Villegas LA,Ram?rez V,Zandi-Nejad K,Mount DB,Torres N,Tovar AR,Romero MF,Gamba G,Plata C

Solute carriers (SLCs) identified and characterized from kidney transcriptome of golden mahseer (Tor putitora) (Fam: Cyprinidae).
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology20054-61(2016 Oct)
Barat A,Sahoo PK,Kumar R,Pande V

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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