ttll12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttll12
Entrez Gene ID	393222
Full Name	tubulin tyrosine ligase-like family, member 12
Synonyms	zgc:55343
General protein information	Preferred Names tubulin tyrosine ligase-like family, member 12 Names tubulin--tyrosine ligase-like protein 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200252.1	NP_956546.1	tubulin--tyrosine ligase-like protein 12

Pathways from BioSystems

Homologies

	TTLL12	XP_001104848.2
Mus musculus (house mouse)	Ttll12	NP_898838.2
Gallus gallus (chicken)	TTLL12	NP_001012558.1
Xenopus tropicalis (tropical clawed frog)	LOC100486512	XP_002944634.2
Pan troglodytes (chimpanzee)	TTLL12	XP_001171890.1
Bos taurus (cattle)	TTLL12	NP_001068978.1
Arabidopsis thaliana (thale cress)	AT1G77550	NP_177879.3
Homo sapiens (human)	TTLL12	NP_055955.1
Rattus norvegicus (Norway rat)	Ttll12	NP_001129394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004284	XP_313558.5
Caenorhabditis elegans (roundworm)	ttll-12	NP_495990.1
Canis lupus familiaris (dog)	TTLL12	XP_531703.4
Danio rerio (zebrafish)	ttll12	NP_956546.1
Drosophila melanogaster (fruit fly)	CG1550	NP_610325.1
Oryza sativa (rice)	Os03g0179000	NP_001049155.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Tubulin tyrosine ligase-like genes ttll3 and ttll6 maintain zebrafish cilia structure and motility.
Pathak N, Austin CA, Drummond IA
The Journal of biological chemistry286(13)11685-95(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ttll12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttll12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31929	NM_200252.1 Latest version!	Danio rerio tubulin tyrosine ligase-like family, member 12 (ttll12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttll12 ( NM_200252.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956546.1 Danio rerio tubulin tyrosine ligase-like family, member 12 (ttll12), mRNA.

Price & Availability↑

CloneID	ODa31929
Clone ID Related Accession (Same CDS sequence)	NM_200252.1
Accession Version	NM_200252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	tubulin--tyrosine ligase-like protein 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048032.1. On Jul 6, 2005 this sequence version replaced XM_691151.1. ##Evidence-Data-START## Transcript exon combination :: BC048032.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAACG GCAGTGTAAA CACAGAGGAG GAAAAAGAGT ATCAGCTCTT CCTCTCTCTG 
CACAGTCCCG CTTTAAAGGC TGCACGAATC CCTCCGCTTT ACTGGAAGAG TTTGCATTTA 
AAACTTGCCA ATGAGGTTTT TGATGCCGGT GATGTGTTTG GCATCATGCA AGTGGAGGAA 
GATGAAGGTG GGGATGAGGA GAAAGAAAAG AGACTCAATC CTGGAGATGA GATCGCTTAT 
AAAGTGATTG TGACCAGAGA GAGTGGTCTG CAGGCTGCGG ATCCCACCAG TGTGTTTCTG 
GTCGATCATG CCTGGACATA TAGGGTGGAA AGCGCACGTC AGCAGCTTCT GGAGATTCCT 
GGCCTGCTGA TCAGAATGGC CAGTCTAATG TGTTTGCCTT TCCACGGAGA GGCTCCAGAT 
CCAGATATTG TGGATCTGAT ACTGGACAGC ATGTGGAAAT ACAACCAAAC CTATCAGCTA 
TCCCAGGGAT CTGCAGAAGA AAAGGTGCCT GTTTGGTACA TCATGGATGA ATTTGGATCT 
CGGGTTCAGC ACTCTTCCCA GCCGACTAGC TGTATGGCTC CTCTGTTTTA CGCTCCGCAA 
CAGATCGCTT ATTCTGTGCT GTGGCCACTG CAGAACCTGG AATATGGAGA TGAGGTGACA 
CGTGATTTTG CGTATGGAGA GACAGACCCT TTAGTCAGGA GATGCCGTCT GCTACCGTGG 
CTGCCTGAAG AACTGGATGC TGTGAGCGAT GACGTGCAGG AACCGTCTGA CCTTTACTAT 
GAAACTATTC TACAGGAGAA CAAAGAAAAG CTTCCCGTAG ACATCCAGCC TTATTGTGTA 
CCAGATGGAA AAGTGCTGAA GGTTTACACT GAGATGAAGC AGGTTTTCGA TCATCTGAAA 
CATCCACGCT TCCAGTTCAC TGAAAATGAG GAAGAGGCTG ACATAATCTG GGCTTTCTCC 
CATATCCGAG AATATCGGAA ACTAAGTGAG GAACGGCCTC ATGTTCTGCT CAACCAGTTC 
CCATGTGAGA CGATTCTTAC TACTAAAGAC TGCTTGGCGT CTGTGGCGCG GAGAGCTGGG 
GGAGGTCAGG AGCCCCCATG GCTCCCTAGA ACCTTCAATT TACAGACTGA GCTGCCGCAG 
TTTATCAGAC ATTACCAGGA GAGGCAAGAG AGAGCGCTGG ATAACCACTG GATCTGCAAG 
CCCTGGAATT TGGCTCGAGG TCTGGACACA CACATCACAG ACAGCCTCGA CTACATAATC 
AGACAGCGAG AGAGCACACC TAAGGTGGTC AGTAAATATC TGGAGGACCC CGTGTTGTTT 
CACAGAGAGG AGGTGGGCAT GGTGAAGTTT GACATCCACT ACATGGTGCT GCTGCGCTCT 
GTTGAACCTC TGCGCCTTTA TGCATACAAC GTCTTCTGGC TCCGTTTTGC TAACAAACCC 
TTCTCTCTAG ATCATTTTGA TGATTATCAG AAGCATTACA CAGTGATGAA TTATGCCGAG 
GATGTGCAGC TCAAGCAGAT TCACTATGAT GAATTCATCC CTATGTTTGA GAGCCAGTAT 
CCGCAGTATG CATGGAAGCA GGTTGAGGCG GATATCTTTA GAGCATTTTC AGAGCTCTTC 
CAGGCAGCGT CCTGTCGTCC CGCTCCATAC GGGATCTGTG CGTATCCCTC CTCCAGAGCC 
ATATACGCCA TCGACCTCAT GCTGAAATGG GACCAGACCG CACAAGGGGA ACGTGTGATG 
CAGCCTCAGA TACTGGAGGT GAATTTTAAC CCAGATTGTA AGAGGGCCTG TCGGTATCAT 
CCCAGCTTTT ACGACCACAT GTTCCAAACG CTGTTCCTGG ATCAGGCAGA AGAATGTCCT 
GTCAGTCACA TTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956546.1
CDS	49..1926
Translation	MQNGSVNTEEEKEYQLFLSLHSPALKAARIPPLYWKSLHLKLANEVFDAGDVFGIMQVEEDEGGDEEKEKRLNPGDEIAYKVIVTRESGLQAADPTSVFLVDHAWTYRVESARQQLLEIPGLLIRMASLMCLPFHGEAPDPDIVDLILDSMWKYNQTYQLSQGSAEEKVPVWYIMDEFGSRVQHSSQPTSCMAPLFYAPQQIAYSVLWPLQNLEYGDEVTRDFAYGETDPLVRRCRLLPWLPEELDAVSDDVQEPSDLYYETILQENKEKLPVDIQPYCVPDGKVLKVYTEMKQVFDHLKHPRFQFTENEEEADIIWAFSHIREYRKLSEERPHVLLNQFPCETILTTKDCLASVARRAGGGQEPPWLPRTFNLQTELPQFIRHYQERQERALDNHWICKPWNLARGLDTHITDSLDYIIRQRESTPKVVSKYLEDPVLFHREEVGMVKFDIHYMVLLRSVEPLRLYAYNVFWLRFANKPFSLDHFDDYQKHYTVMNYAEDVQLKQIHYDEFIPMFESQYPQYAWKQVEADIFRAFSELFQAASCRPAPYGICAYPSSRAIYAIDLMLKWDQTAQGERVMQPQILEVNFNPDCKRACRYHPSFYDHMFQTLFLDQAEECPVSHIL

Target ORF information:

RefSeq Version	NM_200252.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tubulin tyrosine ligase-like family, member 12 (ttll12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200252.1

ORF Insert Sequence:

ATGCAGAACG GCAGTGTAAA CACAGAGGAG GAAAAAGAGT ATCAGCTCTT CCTCTCTCTG 
CACAGTCCCG CTTTAAAGGC TGCACGAATC CCTCCGCTTT ACTGGAAGAG TTTGCATTTA 
AAACTTGCCA ATGAGGTTTT TGATGCCGGT GATGTGTTTG GCATCATGCA AGTGGAGGAA 
GATGAAGGTG GGGATGAGGA GAAAGAAAAG AGACTCAATC CTGGAGATGA GATCGCTTAT 
AAAGTGATTG TGACCAGAGA GAGTGGTCTG CAGGCTGCGG ATCCCACCAG TGTGTTTCTG 
GTCGATCATG CCTGGACATA TAGGGTGGAA AGCGCACGTC AGCAGCTTCT GGAGATTCCT 
GGCCTGCTGA TCAGAATGGC CAGTCTAATG TGTTTGCCTT TCCACGGAGA GGCTCCAGAT 
CCAGATATTG TGGATCTGAT ACTGGACAGC ATGTGGAAAT ACAACCAAAC CTATCAGCTA 
TCCCAGGGAT CTGCAGAAGA AAAGGTGCCT GTTTGGTACA TCATGGATGA ATTTGGATCT 
CGGGTTCAGC ACTCTTCCCA GCCGACTAGC TGTATGGCTC CTCTGTTTTA CGCTCCGCAA 
CAGATCGCTT ATTCTGTGCT GTGGCCACTG CAGAACCTGG AATATGGAGA TGAGGTGACA 
CGTGATTTTG CGTATGGAGA GACAGACCCT TTAGTCAGGA GATGCCGTCT GCTACCGTGG 
CTGCCTGAAG AACTGGATGC TGTGAGCGAT GACGTGCAGG AACCGTCTGA CCTTTACTAT 
GAAACTATTC TACAGGAGAA CAAAGAAAAG CTTCCCGTAG ACATCCAGCC TTATTGTGTA 
CCAGATGGAA AAGTGCTGAA GGTTTACACT GAGATGAAGC AGGTTTTCGA TCATCTGAAA 
CATCCACGCT TCCAGTTCAC TGAAAATGAG GAAGAGGCTG ACATAATCTG GGCTTTCTCC 
CATATCCGAG AATATCGGAA ACTAAGTGAG GAACGGCCTC ATGTTCTGCT CAACCAGTTC 
CCATGTGAGA CGATTCTTAC TACTAAAGAC TGCTTGGCGT CTGTGGCGCG GAGAGCTGGG 
GGAGGTCAGG AGCCCCCATG GCTCCCTAGA ACCTTCAATT TACAGACTGA GCTGCCGCAG 
TTTATCAGAC ATTACCAGGA GAGGCAAGAG AGAGCGCTGG ATAACCACTG GATCTGCAAG 
CCCTGGAATT TGGCTCGAGG TCTGGACACA CACATCACAG ACAGCCTCGA CTACATAATC 
AGACAGCGAG AGAGCACACC TAAGGTGGTC AGTAAATATC TGGAGGACCC CGTGTTGTTT 
CACAGAGAGG AGGTGGGCAT GGTGAAGTTT GACATCCACT ACATGGTGCT GCTGCGCTCT 
GTTGAACCTC TGCGCCTTTA TGCATACAAC GTCTTCTGGC TCCGTTTTGC TAACAAACCC 
TTCTCTCTAG ATCATTTTGA TGATTATCAG AAGCATTACA CAGTGATGAA TTATGCCGAG 
GATGTGCAGC TCAAGCAGAT TCACTATGAT GAATTCATCC CTATGTTTGA GAGCCAGTAT 
CCGCAGTATG CATGGAAGCA GGTTGAGGCG GATATCTTTA GAGCATTTTC AGAGCTCTTC 
CAGGCAGCGT CCTGTCGTCC CGCTCCATAC GGGATCTGTG CGTATCCCTC CTCCAGAGCC 
ATATACGCCA TCGACCTCAT GCTGAAATGG GACCAGACCG CACAAGGGGA ACGTGTGATG 
CAGCCTCAGA TACTGGAGGT GAATTTTAAC CCAGATTGTA AGAGGGCCTG TCGGTATCAT 
CCCAGCTTTT ACGACCACAT GTTCCAAACG CTGTTCCTGG ATCAGGCAGA AGAATGTCCT 
GTCAGTCACA TTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Tubulin tyrosine ligase-like genes ttll3 and ttll6 maintain zebrafish cilia structure and motility.
The Journal of biological chemistry286(13)11685-95(2011 Apr)
Pathak N,Austin CA,Drummond IA