ppp2r5ea cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppp2r5ea
Entrez Gene ID	373881
Full Name	protein phosphatase 2, regulatory subunit B', epsilon isoform a
Synonyms	ppp2r5e2,wdb1,zgc:85613
General protein information	Preferred Names protein phosphatase 2, regulatory subunit B', epsilon isoform a Names protein phosphatase 2, regulatory subunit B', epsilon isoform a protein phosphatase 2, regulatory subunit B (B56) 2 widerborst 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294639.2	XP_009292914.1	protein phosphatase 2, regulatory subunit B', epsilon isoform X1
XM_017352443.1	XP_017207932.1	protein phosphatase 2, regulatory subunit B', epsilon isoform X2
NM_194415.2	NP_919396.1	protein phosphatase 2, regulatory subunit B', epsilon isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPP2R5E	XP_003314413.1
Rattus norvegicus (Norway rat)	Ppp2r5e	NP_001100210.1
Danio rerio (zebrafish)	ppp2r5ea	NP_919396.1
Bos taurus (cattle)	PPP2R5E	NP_001076937.1
Caenorhabditis elegans (roundworm)	pptr-1	NP_507133.4
Arabidopsis thaliana (thale cress)	ATB' ALPHA	NP_195967.1
Drosophila melanogaster (fruit fly)	wdb	NP_733216.1
Arabidopsis thaliana (thale cress)	AT3G54930	NP_191053.1
Arabidopsis thaliana (thale cress)	ATB' BETA	NP_187599.1
Homo sapiens (human)	PPP2R5E	NP_001269108.1
	PPP2R5E	XP_002805110.1
Canis lupus familiaris (dog)	PPP2R5E	XP_005623536.1
Mus musculus (house mouse)	Ppp2r5e	NP_036154.1
Gallus gallus (chicken)	PPP2R5E	XP_421412.2
Danio rerio (zebrafish)	ppp2r5eb	NP_919393.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004096	XP_003436712.1
Xenopus tropicalis (tropical clawed frog)	ppp2r5e	NP_989253.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A.
Hannus M, Feiguin F, Heisenberg CP, Eaton S
Development (Cambridge, England)129(14)3493-503(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In zebrafish, two wdb (protein phosphatase 2, regulatory subunit B (B56)) homologs are required for convergent extension during gastrulation.
Title: Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A.

The following ppp2r5ea gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppp2r5ea cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36769	NM_194415.2 Latest version!	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa40306	XM_009294639.2 Latest version!	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa56545	XM_017352443.1 Latest version!	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppp2r5ea ( NM_194415.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919396.1 Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), mRNA.

Price & Availability↑

CloneID	ODa36769
Clone ID Related Accession (Same CDS sequence)	NM_194415.2
Accession Version	NM_194415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	protein phosphatase 2, regulatory subunit B', epsilon isoform a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078645.1. On Aug 23, 2004 this sequence version replaced NM_194415.1. ##Evidence-Data-START## Transcript exon combination :: BC078645.1, BC044398.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCA 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCTAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGGAAAG GAAGAAGGAC 
AAGGAGCGTG AGGACCTGTG GAGGAAGCTG GAGGATCTGG AGCTCAGACG GGGCATCCAA 
AACAGCGACG GCGTCATTCC CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919396.1
CDS	454..1860
Translation	MSSAATTAPSVDKVDGFSRKSVRKARQKRAQSSSQFRSQDKPIELVALPLLKDVSAQEQPELFLKKLQQCCTLFDFMDTLSDLKMKEYKRSTLNELVDYVTLSRGYLTEQTYPEVVKMVSYNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSAAKKYIDQKFVLQLLELFDSEDPRERDCLKTVLHRIYGKFLGLRAFIRKQINNIFLCFVYETERFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLLPLHTVRSLSLFHAQLAYCIVQFLEKDPTLTEPVIRGLLKFWPKTCSQKEVMFLGELEEILDVIEPTQFVKIQELLFKQIARCVSSPHFQVAERALYYWNNEYIMSLIEENCSAILPIMFASLYRISKEHWNPAISALIYNVLKAFMEMNSALFDELAASYKSDRQRERKKDKEREDLWRKLEDLELRRGIQNSDGVIPT

Target ORF information:

RefSeq Version	NM_194415.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194415.2

ORF Insert Sequence:

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCA 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCTAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGGAAAG GAAGAAGGAC 
AAGGAGCGTG AGGACCTGTG GAGGAAGCTG GAGGATCTGG AGCTCAGACG GGGCATCCAA 
AACAGCGACG GCGTCATTCC CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppp2r5ea ( XM_009294639.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292914.1 Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40306
Clone ID Related Accession (Same CDS sequence)	XM_009294639.2
Accession Version	XM_009294639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase 2, regulatory subunit B', epsilon isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCG 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCCAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGGAAAG GAAGAAGGAC 
AAGGAGCGTG AGGACCTGTG GAGGAAGCTG GAGGATCTGG AGCTCAGACG GGGCATCCAA 
AACAGCGACG GCGTCATTCC CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292914.1
CDS	24..1430
Translation	MSSAATTAPSVDKVDGFSRKSVRKARQKRAQSSSQFRSQDKPIELVALPLLKDVSAQEQPELFLKKLQQCCTLFDFMDTLSDLKMKEYKRSTLNELVDYVTLSRGYLTEQTYPEVVKMVSYNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSAAKKYIDQKFVLQLLELFDSEDPRERDCLKTVLHRIYGKFLGLRAFIRKQINNIFLCFVYETERFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLLPLHTVRSLSLFHAQLAYCIVQFLEKDPTLTEPVIRGLLKFWPKTCSQKEVMFLGELEEILDVIEPTQFVKIQELLFKQIARCVSSPHFQVAERALYYWNNEYIMSLIEENCSAILPIMFASLYRISKEHWNPAISALIYNVLKAFMEMNSALFDELAASYKSDRQRERKKDKEREDLWRKLEDLELRRGIQNSDGVIPT

Target ORF information:

RefSeq Version	XM_009294639.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294639.2

ORF Insert Sequence:

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCG 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCCAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGGAAAG GAAGAAGGAC 
AAGGAGCGTG AGGACCTGTG GAGGAAGCTG GAGGATCTGG AGCTCAGACG GGGCATCCAA 
AACAGCGACG GCGTCATTCC CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppp2r5ea ( XM_017352443.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207932.1 Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56545
Clone ID Related Accession (Same CDS sequence)	XM_017352443.1
Accession Version	XM_017352443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase 2, regulatory subunit B', epsilon isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCG 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCCAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207932.1
CDS	502..1809
Translation	MSSAATTAPSVDKVDGFSRKSVRKARQKRAQSSSQFRSQDKPIELVALPLLKDVSAQEQPELFLKKLQQCCTLFDFMDTLSDLKMKEYKRSTLNELVDYVTLSRGYLTEQTYPEVVKMVSYNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSAAKKYIDQKFVLQLLELFDSEDPRERDCLKTVLHRIYGKFLGLRAFIRKQINNIFLCFVYETERFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLLPLHTVRSLSLFHAQLAYCIVQFLEKDPTLTEPVIRGLLKFWPKTCSQKEVMFLGELEEILDVIEPTQFVKIQELLFKQIARCVSSPHFQVAERALYYWNNEYIMSLIEENCSAILPIMFASLYRISKEHWNPAISALIYNVLKAFMEMNSALFDELAASYKSDRQR

Target ORF information:

RefSeq Version	XM_017352443.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein phosphatase 2, regulatory subunit B', epsilon isoform a (ppp2r5ea), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352443.1

ORF Insert Sequence:

ATGTCTTCAG CAGCCACCAC AGCCCCGTCG GTGGACAAGG TGGACGGCTT CTCCAGGAAG 
TCTGTCAGGA AAGCCCGGCA GAAGCGAGCG CAGAGCTCAT CCCAGTTCAG ATCTCAGGAC 
AAGCCCATAG AGCTGGTGGC GCTGCCTCTG CTGAAAGATG TTTCTGCGCA GGAGCAACCG 
GAGCTGTTCC TCAAGAAGCT GCAGCAGTGC TGTACTCTGT TTGACTTCAT GGATACTCTG 
TCTGACCTCA AAATGAAAGA GTATAAACGT TCCACCCTTA ATGAGCTGGT GGACTACGTC 
ACACTCAGCC GCGGTTACCT GACCGAGCAG ACCTACCCTG AGGTGGTCAA AATGGTGTCC 
TATAATATTT TTCGGACTCT TCCGCCCAGT GACAGTAACG AGTTTGACCC AGAGGAAGAC 
GAGCCCACGC TGGAAGCCTC ATGGCCACAC TTACAGCTTG TTTATGAGTT CTTCATCCGT 
TTCTTGGAAA GTCAGGAATT TCAGCCCAGC GCTGCCAAAA AATACATCGA TCAAAAATTT 
GTTTTACAGC TCTTAGAGCT GTTTGATAGC GAGGACCCAC GAGAGAGGGA CTGTCTGAAG 
ACAGTTCTGC ACAGAATCTA CGGCAAGTTC CTTGGCCTCA GGGCCTTCAT CCGCAAACAA 
ATTAACAACA TTTTTCTTTG TTTTGTGTAT GAGACAGAGC GCTTCAATGG CGTAGCTGAA 
CTGCTGGAGA TTTTGGGAAG CATCATCAAT GGTTTTGCGC TTCCTCTCAA AGCTGAGCAC 
AAACAGTTCT TGGTGAAGGT GCTGCTGCCT CTGCACACAG TGAGGAGCTT ATCTCTCTTC 
CATGCCCAGC TGGCCTATTG TATTGTACAG TTCCTAGAGA AAGACCCCAC GTTAACAGAA 
CCTGTGATCA GAGGTTTGTT GAAGTTTTGG CCAAAAACAT GCAGTCAAAA GGAGGTTATG 
TTTCTTGGAG AGTTGGAGGA GATTCTAGAT GTGATTGAAC CCACTCAGTT TGTTAAGATC 
CAGGAGCTGC TCTTCAAACA GATTGCTAGA TGTGTTTCCA GTCCCCATTT TCAGGTGGCA 
GAGCGAGCCT TGTACTACTG GAACAATGAG TACATCATGA GTCTGATTGA GGAGAACTGT 
AGCGCCATCC TGCCTATCAT GTTTGCCAGT CTCTACAGGA TCTCCAAAGA GCACTGGAAC 
CCGGCGATCT CAGCTTTGAT CTACAATGTG CTCAAAGCAT TCATGGAGAT GAACAGCGCC 
TTGTTTGACG AACTTGCAGC CAGTTACAAA TCAGACAGGC AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A.
Development (Cambridge, England)129(14)3493-503(2002 Jul)
Hannus M,Feiguin F,Heisenberg CP,Eaton S

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ppp2r5ea cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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