nucb2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nucb2a
Entrez Gene ID	373078
Full Name	nucleobindin 2a
Synonyms	cb638,nucb2,wu:fa03d10
General protein information	Preferred Names nucleobindin 2a Names nucleobindin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201493.1	NP_958901.1	nucleobindin-2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NUCB2	NP_001068849.1
Gallus gallus (chicken)	NUCB2	NP_001006468.1
Xenopus tropicalis (tropical clawed frog)	nucb2	NP_001015824.1
Pan troglodytes (chimpanzee)	NUCB2	XP_001172631.1
	NUCB2	XP_002808120.1
Canis lupus familiaris (dog)	NUCB2	XP_005633774.1
Rattus norvegicus (Norway rat)	Nucb2	NP_067695.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005608	XP_315620.4
Danio rerio (zebrafish)	nucb2b	NP_958887.1
Homo sapiens (human)	NUCB2	NP_005004.1
Drosophila melanogaster (fruit fly)	NUCB1	NP_649008.2
Mus musculus (house mouse)	Nucb2	NP_001123951.1
Danio rerio (zebrafish)	nucb2a	NP_958901.1
Caenorhabditis elegans (roundworm)	nucb-1	NP_741886.1

Gene Ontology
Bibliography

Related articles in PubMed

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish.
Falcinelli S, Rodiles A, Hatef A, Picchietti S, Cossignani L, Merrifield DL, Unniappan S, Carnevali O
Scientific reports7(1)5512(2017 Jul)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Ultrasonography reveals in vivo dose-dependent inhibition of end systolic and diastolic volumes, heart rate and cardiac output by nesfatin-1 in zebrafish.
Nair N, Gerger C, Hatef A, Weber LP, Unniappan S
General and comparative endocrinology234142-50(2016 Aug)

Probiotic treatment reduces appetite and glucose level in the zebrafish model.
Falcinelli S, Rodiles A, Unniappan S, Picchietti S, Gioacchini G, Merrifield DL, Carnevali O
Scientific reports618061(2016 Jan)

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain.
Hatef A, Shajan S, Unniappan S
General and comparative endocrinology21551-60(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nucb2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nucb2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37044	NM_201493.1 Latest version!	Danio rerio nucleobindin 2a (nucb2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nucb2a ( NM_201493.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_958901.1 Danio rerio nucleobindin 2a (nucb2a), mRNA.

Price & Availability↑

CloneID	ODa37044
Clone ID Related Accession (Same CDS sequence)	NM_201493.1
Accession Version	NM_201493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleobindin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046077.1. ##Evidence-Data-START## Transcript exon combination :: BC046077.1, BC065437.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTGTT TAAAGGGGCT CCTCACCAGC TGCCTTGTGT TGGCGAGTGT GCTCTCCTGG 
GCCCAAGCAG TGCCAATAAG TATAGATAAA ACAAAGGTGA AGATCCCTGA GGAAACAGTC 
AAAGAGCCCC CTCAGAGTGT GGACACTGGA CTACATTATG ACCGCTATCT CAGAGAAGTT 
ATTGATTTCC TTGAAAAAGA TCAACATTTC AGAGAAAAGC TCCACAACAC GGACATGGAA 
GATATAAAGC AAGGGAAGTT GGCTGAAGAG CTCGACTTTG TCAGCCACCA TGTGCGAAGC 
AAGCTGGATG AACTAAAAAG GCAAGAAGTC AGTCGCCTGA GAACCCTGAT CAAGGCCAAG 
CAAGACATCG AAGGAGGAAA CGATATTGCG GTGGATCACC AGGCGCTACT CAAACAGTTT 
GAATATCTGA ACCACATGAA CCCGCATACA TTTGAGGTTG AGGATCTGGA CAGACTCATC 
AAATCGGCTA CAAAAGATCT GGAGAACTAC GACAAGGAGC GGCATGAAGA ATTTAAAAAG 
TACGAGATGA TGAAAGAGCA CGAGAGAAGG GAACACCTGA AGACACTAGA TGAAGATGGC 
AGGAAAAAAG AGGAAGAACA CTATGAGGAG ATGAAAAAGA AACATGCCGA TCACCCCAAA 
GTCAACCATC CCGGTAGCAA AGACCAGCTA AAAGAAGTGT GGGAGGAGGC AGATGGCCTT 
GACCCTGAAG ATTTTGACCC CAAAACGTTT TTCAACCTGC ATGACACAAA CGGAGATGGC 
TTCTTTGATG AACAAGAACT GGAATCCCTA TTCACTAAGG AGCTGGAGAA GATCTATGAT 
CCAACTAATG AAGAGGATGA CATGGTGGAA ATGGAAGAGG AGAGGCTGCG AATGAGAGAA 
CACGTCATGA ACGAGGTCGA CTCCAATAAA GACAGACTGG TGTCCCTAGA TGAGTTTCTG 
GTTGCCACAA AGAAAAAGGA GTTTCTTGAA CCAGACAGCT GGGAAACTCT GGAACAGAAC 
CAGGCATACA CTGAAGAAGA GATGAGAGAG TTTGAGGAAC AGTTGGTCAG ACAGGAGGAA 
GACCTGAACC AGAAAGCAGC CGATCTTCAG AAGCAGAGAG AGGATCTGGA GCGGCAGCAG 
GAGCAGCTCA ATGCACAGAA AATTGAGCTG CAGCAAGCTG TGGAGCATAT GGAGAGGATA 
AAAACACAAA AGGTGCCACC TCCATCTGAG ATCTTGGAAG GAAATGCAGT CCCAGAGTCA 
GTAGGACAAG ACCAGCCTCC AGTTCCCCTT GAGCACCAGC CATTACCCCC AGGACATCAA 
GATGTCCCAC CACCAGCAGC ACAACAACAT CACGATGAAC TCCAGAATCA ACAAGCTCTA 
GGACAGGAAC ACCAAAATAC ACCCCATGAA AACCAGCCAC TGCCTCCAGG ACACAACAAC 
CCACCCGAAG ACCCTCCACT GGTGCCCCTT GACCACAACA TTGTGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958901.1
CDS	106..1596
Translation	MSCLKGLLTSCLVLASVLSWAQAVPISIDKTKVKIPEETVKEPPQSVDTGLHYDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAEELDFVSHHVRSKLDELKRQEVSRLRTLIKAKQDIEGGNDIAVDHQALLKQFEYLNHMNPHTFEVEDLDRLIKSATKDLENYDKERHEEFKKYEMMKEHERREHLKTLDEDGRKKEEEHYEEMKKKHADHPKVNHPGSKDQLKEVWEEADGLDPEDFDPKTFFNLHDTNGDGFFDEQELESLFTKELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDSNKDRLVSLDEFLVATKKKEFLEPDSWETLEQNQAYTEEEMREFEEQLVRQEEDLNQKAADLQKQREDLERQQEQLNAQKIELQQAVEHMERIKTQKVPPPSEILEGNAVPESVGQDQPPVPLEHQPLPPGHQDVPPPAAQQHHDELQNQQALGQEHQNTPHENQPLPPGHNNPPEDPPLVPLDHNIVP

Target ORF information:

RefSeq Version	NM_201493.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleobindin 2a (nucb2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201493.1

ORF Insert Sequence:

ATGTCCTGTT TAAAGGGGCT CCTCACCAGC TGCCTTGTGT TGGCGAGTGT GCTCTCCTGG 
GCCCAAGCAG TGCCAATAAG TATAGATAAA ACAAAGGTGA AGATCCCTGA GGAAACAGTC 
AAAGAGCCCC CTCAGAGTGT GGACACTGGA CTACATTATG ACCGCTATCT CAGAGAAGTT 
ATTGATTTCC TTGAAAAAGA TCAACATTTC AGAGAAAAGC TCCACAACAC GGACATGGAA 
GATATAAAGC AAGGGAAGTT GGCTGAAGAG CTCGACTTTG TCAGCCACCA TGTGCGAAGC 
AAGCTGGATG AACTAAAAAG GCAAGAAGTC AGTCGCCTGA GAACCCTGAT CAAGGCCAAG 
CAAGACATCG AAGGAGGAAA CGATATTGCG GTGGATCACC AGGCGCTACT CAAACAGTTT 
GAATATCTGA ACCACATGAA CCCGCATACA TTTGAGGTTG AGGATCTGGA CAGACTCATC 
AAATCGGCTA CAAAAGATCT GGAGAACTAC GACAAGGAGC GGCATGAAGA ATTTAAAAAG 
TACGAGATGA TGAAAGAGCA CGAGAGAAGG GAACACCTGA AGACACTAGA TGAAGATGGC 
AGGAAAAAAG AGGAAGAACA CTATGAGGAG ATGAAAAAGA AACATGCCGA TCACCCCAAA 
GTCAACCATC CCGGTAGCAA AGACCAGCTA AAAGAAGTGT GGGAGGAGGC AGATGGCCTT 
GACCCTGAAG ATTTTGACCC CAAAACGTTT TTCAACCTGC ATGACACAAA CGGAGATGGC 
TTCTTTGATG AACAAGAACT GGAATCCCTA TTCACTAAGG AGCTGGAGAA GATCTATGAT 
CCAACTAATG AAGAGGATGA CATGGTGGAA ATGGAAGAGG AGAGGCTGCG AATGAGAGAA 
CACGTCATGA ACGAGGTCGA CTCCAATAAA GACAGACTGG TGTCCCTAGA TGAGTTTCTG 
GTTGCCACAA AGAAAAAGGA GTTTCTTGAA CCAGACAGCT GGGAAACTCT GGAACAGAAC 
CAGGCATACA CTGAAGAAGA GATGAGAGAG TTTGAGGAAC AGTTGGTCAG ACAGGAGGAA 
GACCTGAACC AGAAAGCAGC CGATCTTCAG AAGCAGAGAG AGGATCTGGA GCGGCAGCAG 
GAGCAGCTCA ATGCACAGAA AATTGAGCTG CAGCAAGCTG TGGAGCATAT GGAGAGGATA 
AAAACACAAA AGGTGCCACC TCCATCTGAG ATCTTGGAAG GAAATGCAGT CCCAGAGTCA 
GTAGGACAAG ACCAGCCTCC AGTTCCCCTT GAGCACCAGC CATTACCCCC AGGACATCAA 
GATGTCCCAC CACCAGCAGC ACAACAACAT CACGATGAAC TCCAGAATCA ACAAGCTCTA 
GGACAGGAAC ACCAAAATAC ACCCCATGAA AACCAGCCAC TGCCTCCAGG ACACAACAAC 
CCACCCGAAG ACCCTCCACT GGTGCCCCTT GACCACAACA TTGTGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish.
Scientific reports7(1)5512(2017 Jul)
Falcinelli S,Rodiles A,Hatef A,Picchietti S,Cossignani L,Merrifield DL,Unniappan S,Carnevali O

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Ultrasonography reveals in vivo dose-dependent inhibition of end systolic and diastolic volumes, heart rate and cardiac output by nesfatin-1 in zebrafish.
General and comparative endocrinology234142-50(2016 Aug)
Nair N,Gerger C,Hatef A,Weber LP,Unniappan S

Probiotic treatment reduces appetite and glucose level in the zebrafish model.
Scientific reports618061(2016 Jan)
Falcinelli S,Rodiles A,Unniappan S,Picchietti S,Gioacchini G,Merrifield DL,Carnevali O

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain.
General and comparative endocrinology21551-60(2015 May)
Hatef A,Shajan S,Unniappan S