phlda3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phlda3
Entrez Gene ID	368779
Full Name	pleckstrin homology-like domain, family A, member 3
Synonyms	si:dz182n13.5,zgc:92333
General protein information	Preferred Names pleckstrin homology-like domain, family A, member 3 Names pleckstrin homology-like domain family A member 3 TDAG51/Ipl homolog 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002455.1	NP_001002455.1	pleckstrin homology-like domain family A member 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHLDA3	XP_003308734.1
	LOC709782	XP_001098353.1
Rattus norvegicus (Norway rat)	Phlda3	NP_001012206.1
Homo sapiens (human)	PHLDA3	NP_036528.1
Mus musculus (house mouse)	Phlda3	NP_038778.1
Gallus gallus (chicken)	LOC100859283	XP_003642782.1
Danio rerio (zebrafish)	phlda3	NP_001002455.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Loponte S, Segr? CV, Senese S, Miccolo C, Santaguida S, Deflorian G, Citro S, Mattoscio D, Pisati F, Moser MA, Visintin R, Seiser C, Chiocca S
Scientific reports630213(2016 Jul)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Direct regulation of p53 by miR-142a-3p mediates the survival of hematopoietic stem and progenitor cells in zebrafish.
Lu X, Wei Y, Liu F
Cell discovery115027(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following phlda3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phlda3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37815	NM_001002455.1 Latest version!	Danio rerio pleckstrin homology-like domain, family A, member 3 (phlda3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phlda3 ( NM_001002455.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002455.1 Danio rerio pleckstrin homology-like domain, family A, member 3 (phlda3), mRNA.

Price & Availability↑

CloneID	ODa37815
Clone ID Related Accession (Same CDS sequence)	NM_001002455.1
Accession Version	NM_001002455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology-like domain family A member 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076016.1. ##Evidence-Data-START## Transcript exon combination :: BC076016.1, EV759720.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505374, SAMEA4476817 [ECO:0000348] ##Evidence-Data-END##

ATGAACCAGT GTAAAGTAAT GAATGACGGG TATCTGGAGA AGAGGAGTAA TGGACTTCTC 
CAGCTGTGGA AGAAGAAGCG CTGCGTTCTG TCAGACGAAG GTCTCCGACT GTACGGCTGT 
AAAGGCGACA GCGGTAAAGA GATGCGCTTC GAGCAGATGA CCACTCTGGA TTGTGTGGAG 
TATAAACGGG GTCTGGTGTA CTTCACCATC GTGATGAACG ATGGTAAAGA GGTCGACTTC 
AGGTGCCAGC AGGAGGGAAC GGCGTGGAAC GCAGAGATCG CGCTTGCGCT CGTGCGCTTC 
AAGAACCGCG TCGCCGTTCA GACCGGCAGG AACAGGCATT TGTCACATCT GGGCAGCTGC 
GGGGAAGGAG ACGTCGAACT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002455.1
CDS	221..604
Translation	MNQCKVMNDGYLEKRSNGLLQLWKKKRCVLSDEGLRLYGCKGDSGKEMRFEQMTTLDCVEYKRGLVYFTIVMNDGKEVDFRCQQEGTAWNAEIALALVRFKNRVAVQTGRNRHLSHLGSCGEGDVEL

Target ORF information:

RefSeq Version	NM_001002455.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology-like domain, family A, member 3 (phlda3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002455.1

ORF Insert Sequence:

ATGAACCAGT GTAAAGTAAT GAATGACGGG TATCTGGAGA AGAGGAGTAA TGGACTTCTC 
CAGCTGTGGA AGAAGAAGCG CTGCGTTCTG TCAGACGAAG GTCTCCGACT GTACGGCTGT 
AAAGGCGACA GCGGTAAAGA GATGCGCTTC GAGCAGATGA CCACTCTGGA TTGTGTGGAG 
TATAAACGGG GTCTGGTGTA CTTCACCATC GTGATGAACG ATGGTAAAGA GGTCGACTTC 
AGGTGCCAGC AGGAGGGAAC GGCGTGGAAC GCAGAGATCG CGCTTGCGCT CGTGCGCTTC 
AAGAACCGCG TCGCCGTTCA GACCGGCAGG AACAGGCATT TGTCACATCT GGGCAGCTGC 
GGGGAAGGAG ACGTCGAACT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Scientific reports630213(2016 Jul)
Loponte S,Segr? CV,Senese S,Miccolo C,Santaguida S,Deflorian G,Citro S,Mattoscio D,Pisati F,Moser MA,Visintin R,Seiser C,Chiocca S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Direct regulation of p53 by miR-142a-3p mediates the survival of hematopoietic stem and progenitor cells in zebrafish.
Cell discovery115027(2015)
Lu X,Wei Y,Liu F