itsn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	itsn1
Entrez Gene ID	368504
Full Name	intersectin 1 (SH3 domain protein)
Synonyms	SH3,si:dz173a8.1
General protein information	Preferred Names intersectin 1 (SH3 domain protein) Names intersectin-1 SH3 domain
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170971.4	XP_005171028.1	intersectin-1 isoform X1
XM_005170974.4	XP_005171031.1	intersectin-1 isoform X3
NM_207182.1	NP_997065.1	intersectin-1
XM_005170974.3	XP_005171031.1	intersectin-1 isoform X3
XM_005170971.3	XP_005171028.1	intersectin-1 isoform X1
XM_005170972.3	XP_005171029.1	intersectin-1 isoform X2
XM_005170972.4	XP_005171029.1	intersectin-1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITSN1	NP_003015.2
Canis lupus familiaris (dog)	ITSN1	XP_005638937.1
Drosophila melanogaster (fruit fly)	Dap160	NP_001246118.1
Xenopus tropicalis (tropical clawed frog)	itsn1	XP_002939390.1
Bos taurus (cattle)	ITSN1	XP_002707731.1
Mus musculus (house mouse)	Itsn1	NP_034717.2
Gallus gallus (chicken)	ITSN1	XP_416715.2
	ITSN1	XP_002803192.1
Rattus norvegicus (Norway rat)	Itsn1	NP_001129568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009037	XP_319789.4
Pan troglodytes (chimpanzee)	ITSN1	XP_001166935.1
Canis lupus familiaris (dog)	LOC100856635	XP_003640127.1
Danio rerio (zebrafish)	itsn1	NP_997065.1
Caenorhabditis elegans (roundworm)	itsn-1	NP_001255927.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Dergai M, Tsyba L, Dergai O, Zlatskii I, Skrypkina I, Kovalenko V, Rynditch A
Biochemical and biophysical research communications399(2)307-12(2010 Aug)

olig1 Expression identifies developing oligodendrocytes in zebrafish and requires hedgehog and notch signaling.
Schebesta M, Serluca FC
Developmental dynamics : an official publication of the American Association of Anatomists238(4)887-98(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following itsn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itsn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01709	NM_207182.1 Latest version!	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa06106	XM_005170971.4 Latest version!	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa06107	XM_005170972.4 Latest version!	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa06109	XM_005170974.4 Latest version!	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa06109	XM_005170974.3	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa06106	XM_005170971.3	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa06107	XM_005170972.3	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itsn1 ( NM_207182.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997065.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), mRNA.

Price & Availability↑

CloneID	ODa01709
Clone ID Related Accession (Same CDS sequence)	NM_207182.1
Accession Version	NM_207182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	intersectin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL606751.5. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCTCTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG AGTGCCAGGG 
ATGGGTGTTT CTCCTCCACT GGGCGCATCG GTAACCCCAC CCGTACCATC CCTCGCCAAC 
GGAGCACCCG CAATGATACA ACCCCTGAGT GGATTTTCAC ATCCAGGTGC CGTGCTCAAC 
AAAACCCCCT CATTCAATCG CTCCAGTATC AAGCTACAGA AGGGCCAGTC TGTAGAGGCG 
CCCAGTGCTC CAGCTGCTCC TCCACCCGCT GACTGGGCCG TGTCTCAGTC CTCCAGACTT 
AAGTATCGCC AGCTCTTTAA CAGCCATGAT AAAATGATGA GCGGTTATCT CACAGGTCCA 
CAAGCCAGAA CTATTCTGAT GCAGTCCAGT CTACCACAAG CCCAGCTGGC CACCATCTGG 
AATCTGTCAG ATATAGATCA GGACGGGAAG TTGACTGCTG AAGAGTTTAT TTTGGCCATG 
CACTTGATCG ACATGGCCAT GTCTGGTCTC CCTCTTCCCC CTTTGCTGCC GCCTGACCTT 
ATACCTCCAA CATTCAGGAG AATGCGCTCT GGTAGTGGAG TGTCTGTGAC CAGCATGCAC 
TCGACAGACT TGCGGTCTCA GGAAGAGCCA GAGGAGGAGG AGAAAGAGGC CGAGAAAAAA 
CTTCCAGTCA CATTTGAGGA TAAGAAACGA GAGAACTTTG AGCGCGGAAA CCTGGAGTTG 
GAGAAACGTC GACAGGCACT TCTGGAGCAG CAGAGGAAAG AGCAGGAGCG ACTGGCGGCG 
CTGGAGAAAG AAGAGCAGGA GCGGAAGGAG AGGGAACGAC TGGAGCAGGA GAGACGCAGA 
CAGCAGGAGC TGGACAAACA GCTGGAGAGA CAGAGGGAGC TGGAGCGACA GCGGGAGGAG 
GAGAGACGCA AGGAGATCGA GAGAAGAGAG GCTGCTAAAC GGGAGCTGGA GCGACAGAGG 
CAGCTGGAGT GGGAGAGGAC GCGCAGACAG GAGCTGCTCA CCCAAAGGAA CAGAGAACAG 
GAGAACATCG TCCTGCTTAA AGCACGGAAG AAAACTCTCG AGTTTGAGCT GGAGGCTCTG 
AATGATAAGA AGTCTCAGTT GGAGGGGAAA CTGCAGGATA TTCGGTCCCG TCTCTCGATT 
CAGAGGCATG AGATCGAGAC CACCAATAAG ACTCGGGAGC TCCGCATTGC AGAGATCACA 
AGCCTGCAAC AGCAGCTGCA GGAGTCTCAG CAGTGGTTGA GTCGCTTGAT TCCTGATAAA 
CAGTGTCTGA ATGACCAGCT GAAGCAGGTT CAGCAGAACA GTCTGCACAG AGACTCTTTG 
AACAGTCTTC AGAGGGCCAT TGAAATGAAA GAATCCACCA AACAGCAGCT GAAAGAACAG 
CTGGATGCTG TGGAGAAGGA AACTCGCTCC AAACTATTGG AGATCGACGC CTTCAACACA 
CAGCTGAAGG AGCTGAGAGA AATCCACAAC AAACAGCAGA GGCAGAAACA GAAGGAGCTG 
GAGGCTGACA TCACCCAGCC GTCACATGAC AGGAAGTCCA TAGACTCACA AGACTCGAGG 
TTGTCGGGGA CTGATGATGG CGTGTCTCCA GCGTGGAGGG ATGATGGGCT GGGTAAAGCT 
CCTACTCCTC CTGTGTCTCA GGCCTGGATG AGCCGGGTGA GAGAAAAGAA GATTGTCGAT 
GCAGAAAGAC ATGGATTTGA TCAATTCTGC TGCTGCTTTA TGAGAGTAAA TGTCAGAAAT 
CAACATAAGT CATGTGTTTG TGTTCTAGAT AAGATCCCAG TGTCTGGTTT TAATCAGGAG 
AAGGTAAAGG TGGTCTACTA TAGAGCAATG TATCCCTTTG AGGCCCGCAG TCATGATGAG 
ATCACCATCC ATCCTGGAGA CATAGTCATG GTGGATGAGT CTCAGACAGG TGAGCCTGGA 
TGGTTAGGAG GAGAGATTAA AGGGAAGACT GGCTGGTTTC CTGCCAACTA TGCGGAGAAG 
ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG AGGGCCAGCG CCGCTTCTAG CTCCGCCCCT 
AAATTGGGTT CTCACATGTC ATCTGCTTCT TCATCGACCA CGGCCACGCC CATTCTGCCA 
GTCTCCACAG AGCCTGCATC GATAGCCCCG CCCTCTTCAG CCCCACCCCC CACAGGCCCT 
GCCTCCTCCT CTTCCTCAGC ATCCAGCAAT TGGGCAGATT TCAGCACTAC ATGGCCCTCT 
AATTCTGCTG TGGAGAAGCA GGATAGTGAT GGATGGGATG CATGGCCGAC TCAGCCAACT 
CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG CAGATCAGAC AGCGCTCTGC TTTCACGCCT 
GCTACACTCT CCGGCTCCTC GCCCTCACCC GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT 
CTGCAGGCTC AGGCGCTGTA CCCATGGAGA GCCAAGAAAG ACAATCATCT AAACTTCAAC 
AAGAATGACG TGATCACTGT ACTGGAGCAG CAGGACATGT GGTGGTTTGG GGAGGTGCAA 
GGACAGAGAG GATGGTTCCC TAAATCTTAC GTAAAGCTCA TCTCTGGGCC TGTCCGGAAA 
TCTATGAGTA TTGATTCTGG CTCTTCAGAC AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC 
TCTCTAAACA AACCCACAGA GCTCGGAGAA GGTCAGAACT CCAACAGCAA CTCAATGTAT 
CCGTCTAAAG AGTATGTGGC CATGTATACA TATGAAAGCA ATGAGCAGGG TGACTTGACC 
TTCCAGCAAG GTGATGTCAT CACAGTCACC AAGAAAGAAG GAGATTGGTG GACCGGCACT 
GTGAGTGGGA AGATCGGAGT CTTTCCATCG AATTATGTCA AACCTAAAGA ATCCGAGGGG 
TTGGGATCTG CTGGGAAAAC AGGAAGTTTA GGGAAGAAAC CAGAGATTGC CCAAGTCATC 
GCTCCATACA CGGCCACTGG TGCAGAGCAG CTCACTTTAG CACCAGGCCA GCTCATTCTC 
ATTCGCAAGA AAAACCCGGG AGGATGGTGG GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG 
CGGCAGATTG GCTGGTTTCC TGCTAATTAT GTCAAATTAT TGAGTCCCAG TACCAGTAAG 
ACAACACCCA CAGATCCCAA CCCACCCAAG CTGCCGACAC CCAATGCAGT GTGCCAGGTG 
ATTGGCATGT ACGATTACAC TGCTCAGAAT GATGACGAGC TGCCTTTCGG GAAGGGCCAA 
ATCATCAATG TGTTGAGCAG AGAGGATCCC GATTGGTGGA AAGGAGAGTT GAATGGATCC 
GTCGGCCTCT TCCCCTCGAA CTACGTCAAG CTGACCACCG ACACAGACCC CAGCCAACAA 
TGGTGTGCTG ATCTCCACCT GCTCGACATG CTGACCCCAG TGGAGCGGAA GAGGCAGGGA 
TACATACATG AGCTTATTGT TACAGAGGAG AACTATGTCA ATGACCTGCA GCTGGTCACT 
GAGACCTTCC AGAAGCCTCT GCTGGAGTCT GATTTGTTGA CGGAGAAGGA GGTTGCCATG 
ATCTTTGTCA ACTGGAAAGA GCTGACCATG TGCAACATTA AACTGCTCAA GGCGCTGCGG 
GTGAGGAAGA AGATGTCAGG TGAGCGGATG CCAGTGAAGA TGATTGGTGA CATCCTGACG 
GCTCAGCTTC CTCACATGCA GCCGTACATC CGCTTCTGCA GCTGCCAGCT CAACGGCGCC 
ACCCTCATTC AGCAGAAAAC CGACGAGGTG CCCGAATTCA AGGACTTTGT GAAGAGGTTA 
GCGATGGACC CTCGCTGTAA GGGAATGCCC CTGTCCAGTT TCCTTCTCAA ACCCATGCAG 
AGAGTCACCC GATACCCCCT CATCATCAAA AACATTTTAG AGAACACTCC AGAAAGCCAC 
CCAGACCACA GTCACCTGCG TCTGGCTCTG GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC 
AATGAAGGTG TACGAGAGAA AGAAAACTCC GACCGGCTGG AATGGATACA GGCACATGTG 
CAGTGTGAGG GACTGTCAGA GCAACTTGTG TTCAACTCTG TAACAAACTG TCTGGGCCCT 
CGGAAGTTCC TGCACAGTGG AAAACTCTAC AAAGCCAAGA GCAACAAAGA GCTTTACGGC 
TTCCTTTTTA ACGACTTCCT GCTCCTCACA CAGATAATCA AACCTCTGGG CTCATCCGGG 
ACAGACAAGG TCTTCAGTGC GAAATCCCAT CTGCAGTATC GCATGTACAA AACGCCCATC 
TTTCTCAATG AGGTGCTAGT GAAGCTGCCT ACTGACCCCT CGGGAGATGA ACCCATTTTT 
CACATATCTC ACATCGACAG AGTCTACACT ATACGGGCTG AGAGCATCAA CGAAAGGACA 
GCTTGGGTGC AGAAAATCAA AGCTGCCTCT GAGCTCTTCA TCGAAACCGA AAAGAGGAAG 
AGAGAAAAAG CCTACTTGGT GAGGTCTCAG AGGGCCACTG GCATCGGGCG GCTAATGGTC 
AACATTGTTG AAGGCATCGA ACTGAAGCCC TGCCGCTCAA ATGGTAAGAG TAACCCGTAC 
TGTGAGGTGA CGATGGGCTC TCAGTGTCAC GTCACCAAGA CCCTTCAGGA CACTCTGAAC 
CCCAAGTGGA ACTCAAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA CGTGCTGTGT 
GTCACTGTGT TTGAGAGGGA TCAGTTCTCC CCGGACGATT TCTTGGGCAG GACGGAGATT 
CGGCTGGCCG ACATCAAGAA GGACCAGGGC TCGAAGGGTC CCATCACCAA GCGTCTGCTT 
TTACACGAAG TCCCGACGGG AGAGATTGTG GTGCGACTGG ACCTGCAGCT CTTCGATGAA 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997065.1
CDS	32..5197
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVREKKIVDAERHGFDQFCCCFMRVNVRNQHKSCVCVLDKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIDSGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDES

Target ORF information:

RefSeq Version	NM_207182.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207182.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCTCTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG AGTGCCAGGG 
ATGGGTGTTT CTCCTCCACT GGGCGCATCG GTAACCCCAC CCGTACCATC CCTCGCCAAC 
GGAGCACCCG CAATGATACA ACCCCTGAGT GGATTTTCAC ATCCAGGTGC CGTGCTCAAC 
AAAACCCCCT CATTCAATCG CTCCAGTATC AAGCTACAGA AGGGCCAGTC TGTAGAGGCG 
CCCAGTGCTC CAGCTGCTCC TCCACCCGCT GACTGGGCCG TGTCTCAGTC CTCCAGACTT 
AAGTATCGCC AGCTCTTTAA CAGCCATGAT AAAATGATGA GCGGTTATCT CACAGGTCCA 
CAAGCCAGAA CTATTCTGAT GCAGTCCAGT CTACCACAAG CCCAGCTGGC CACCATCTGG 
AATCTGTCAG ATATAGATCA GGACGGGAAG TTGACTGCTG AAGAGTTTAT TTTGGCCATG 
CACTTGATCG ACATGGCCAT GTCTGGTCTC CCTCTTCCCC CTTTGCTGCC GCCTGACCTT 
ATACCTCCAA CATTCAGGAG AATGCGCTCT GGTAGTGGAG TGTCTGTGAC CAGCATGCAC 
TCGACAGACT TGCGGTCTCA GGAAGAGCCA GAGGAGGAGG AGAAAGAGGC CGAGAAAAAA 
CTTCCAGTCA CATTTGAGGA TAAGAAACGA GAGAACTTTG AGCGCGGAAA CCTGGAGTTG 
GAGAAACGTC GACAGGCACT TCTGGAGCAG CAGAGGAAAG AGCAGGAGCG ACTGGCGGCG 
CTGGAGAAAG AAGAGCAGGA GCGGAAGGAG AGGGAACGAC TGGAGCAGGA GAGACGCAGA 
CAGCAGGAGC TGGACAAACA GCTGGAGAGA CAGAGGGAGC TGGAGCGACA GCGGGAGGAG 
GAGAGACGCA AGGAGATCGA GAGAAGAGAG GCTGCTAAAC GGGAGCTGGA GCGACAGAGG 
CAGCTGGAGT GGGAGAGGAC GCGCAGACAG GAGCTGCTCA CCCAAAGGAA CAGAGAACAG 
GAGAACATCG TCCTGCTTAA AGCACGGAAG AAAACTCTCG AGTTTGAGCT GGAGGCTCTG 
AATGATAAGA AGTCTCAGTT GGAGGGGAAA CTGCAGGATA TTCGGTCCCG TCTCTCGATT 
CAGAGGCATG AGATCGAGAC CACCAATAAG ACTCGGGAGC TCCGCATTGC AGAGATCACA 
AGCCTGCAAC AGCAGCTGCA GGAGTCTCAG CAGTGGTTGA GTCGCTTGAT TCCTGATAAA 
CAGTGTCTGA ATGACCAGCT GAAGCAGGTT CAGCAGAACA GTCTGCACAG AGACTCTTTG 
AACAGTCTTC AGAGGGCCAT TGAAATGAAA GAATCCACCA AACAGCAGCT GAAAGAACAG 
CTGGATGCTG TGGAGAAGGA AACTCGCTCC AAACTATTGG AGATCGACGC CTTCAACACA 
CAGCTGAAGG AGCTGAGAGA AATCCACAAC AAACAGCAGA GGCAGAAACA GAAGGAGCTG 
GAGGCTGACA TCACCCAGCC GTCACATGAC AGGAAGTCCA TAGACTCACA AGACTCGAGG 
TTGTCGGGGA CTGATGATGG CGTGTCTCCA GCGTGGAGGG ATGATGGGCT GGGTAAAGCT 
CCTACTCCTC CTGTGTCTCA GGCCTGGATG AGCCGGGTGA GAGAAAAGAA GATTGTCGAT 
GCAGAAAGAC ATGGATTTGA TCAATTCTGC TGCTGCTTTA TGAGAGTAAA TGTCAGAAAT 
CAACATAAGT CATGTGTTTG TGTTCTAGAT AAGATCCCAG TGTCTGGTTT TAATCAGGAG 
AAGGTAAAGG TGGTCTACTA TAGAGCAATG TATCCCTTTG AGGCCCGCAG TCATGATGAG 
ATCACCATCC ATCCTGGAGA CATAGTCATG GTGGATGAGT CTCAGACAGG TGAGCCTGGA 
TGGTTAGGAG GAGAGATTAA AGGGAAGACT GGCTGGTTTC CTGCCAACTA TGCGGAGAAG 
ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG AGGGCCAGCG CCGCTTCTAG CTCCGCCCCT 
AAATTGGGTT CTCACATGTC ATCTGCTTCT TCATCGACCA CGGCCACGCC CATTCTGCCA 
GTCTCCACAG AGCCTGCATC GATAGCCCCG CCCTCTTCAG CCCCACCCCC CACAGGCCCT 
GCCTCCTCCT CTTCCTCAGC ATCCAGCAAT TGGGCAGATT TCAGCACTAC ATGGCCCTCT 
AATTCTGCTG TGGAGAAGCA GGATAGTGAT GGATGGGATG CATGGCCGAC TCAGCCAACT 
CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG CAGATCAGAC AGCGCTCTGC TTTCACGCCT 
GCTACACTCT CCGGCTCCTC GCCCTCACCC GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT 
CTGCAGGCTC AGGCGCTGTA CCCATGGAGA GCCAAGAAAG ACAATCATCT AAACTTCAAC 
AAGAATGACG TGATCACTGT ACTGGAGCAG CAGGACATGT GGTGGTTTGG GGAGGTGCAA 
GGACAGAGAG GATGGTTCCC TAAATCTTAC GTAAAGCTCA TCTCTGGGCC TGTCCGGAAA 
TCTATGAGTA TTGATTCTGG CTCTTCAGAC AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC 
TCTCTAAACA AACCCACAGA GCTCGGAGAA GGTCAGAACT CCAACAGCAA CTCAATGTAT 
CCGTCTAAAG AGTATGTGGC CATGTATACA TATGAAAGCA ATGAGCAGGG TGACTTGACC 
TTCCAGCAAG GTGATGTCAT CACAGTCACC AAGAAAGAAG GAGATTGGTG GACCGGCACT 
GTGAGTGGGA AGATCGGAGT CTTTCCATCG AATTATGTCA AACCTAAAGA ATCCGAGGGG 
TTGGGATCTG CTGGGAAAAC AGGAAGTTTA GGGAAGAAAC CAGAGATTGC CCAAGTCATC 
GCTCCATACA CGGCCACTGG TGCAGAGCAG CTCACTTTAG CACCAGGCCA GCTCATTCTC 
ATTCGCAAGA AAAACCCGGG AGGATGGTGG GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG 
CGGCAGATTG GCTGGTTTCC TGCTAATTAT GTCAAATTAT TGAGTCCCAG TACCAGTAAG 
ACAACACCCA CAGATCCCAA CCCACCCAAG CTGCCGACAC CCAATGCAGT GTGCCAGGTG 
ATTGGCATGT ACGATTACAC TGCTCAGAAT GATGACGAGC TGCCTTTCGG GAAGGGCCAA 
ATCATCAATG TGTTGAGCAG AGAGGATCCC GATTGGTGGA AAGGAGAGTT GAATGGATCC 
GTCGGCCTCT TCCCCTCGAA CTACGTCAAG CTGACCACCG ACACAGACCC CAGCCAACAA 
TGGTGTGCTG ATCTCCACCT GCTCGACATG CTGACCCCAG TGGAGCGGAA GAGGCAGGGA 
TACATACATG AGCTTATTGT TACAGAGGAG AACTATGTCA ATGACCTGCA GCTGGTCACT 
GAGACCTTCC AGAAGCCTCT GCTGGAGTCT GATTTGTTGA CGGAGAAGGA GGTTGCCATG 
ATCTTTGTCA ACTGGAAAGA GCTGACCATG TGCAACATTA AACTGCTCAA GGCGCTGCGG 
GTGAGGAAGA AGATGTCAGG TGAGCGGATG CCAGTGAAGA TGATTGGTGA CATCCTGACG 
GCTCAGCTTC CTCACATGCA GCCGTACATC CGCTTCTGCA GCTGCCAGCT CAACGGCGCC 
ACCCTCATTC AGCAGAAAAC CGACGAGGTG CCCGAATTCA AGGACTTTGT GAAGAGGTTA 
GCGATGGACC CTCGCTGTAA GGGAATGCCC CTGTCCAGTT TCCTTCTCAA ACCCATGCAG 
AGAGTCACCC GATACCCCCT CATCATCAAA AACATTTTAG AGAACACTCC AGAAAGCCAC 
CCAGACCACA GTCACCTGCG TCTGGCTCTG GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC 
AATGAAGGTG TACGAGAGAA AGAAAACTCC GACCGGCTGG AATGGATACA GGCACATGTG 
CAGTGTGAGG GACTGTCAGA GCAACTTGTG TTCAACTCTG TAACAAACTG TCTGGGCCCT 
CGGAAGTTCC TGCACAGTGG AAAACTCTAC AAAGCCAAGA GCAACAAAGA GCTTTACGGC 
TTCCTTTTTA ACGACTTCCT GCTCCTCACA CAGATAATCA AACCTCTGGG CTCATCCGGG 
ACAGACAAGG TCTTCAGTGC GAAATCCCAT CTGCAGTATC GCATGTACAA AACGCCCATC 
TTTCTCAATG AGGTGCTAGT GAAGCTGCCT ACTGACCCCT CGGGAGATGA ACCCATTTTT 
CACATATCTC ACATCGACAG AGTCTACACT ATACGGGCTG AGAGCATCAA CGAAAGGACA 
GCTTGGGTGC AGAAAATCAA AGCTGCCTCT GAGCTCTTCA TCGAAACCGA AAAGAGGAAG 
AGAGAAAAAG CCTACTTGGT GAGGTCTCAG AGGGCCACTG GCATCGGGCG GCTAATGGTC 
AACATTGTTG AAGGCATCGA ACTGAAGCCC TGCCGCTCAA ATGGTAAGAG TAACCCGTAC 
TGTGAGGTGA CGATGGGCTC TCAGTGTCAC GTCACCAAGA CCCTTCAGGA CACTCTGAAC 
CCCAAGTGGA ACTCAAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA CGTGCTGTGT 
GTCACTGTGT TTGAGAGGGA TCAGTTCTCC CCGGACGATT TCTTGGGCAG GACGGAGATT 
CGGCTGGCCG ACATCAAGAA GGACCAGGGC TCGAAGGGTC CCATCACCAA GCGTCTGCTT 
TTACACGAAG TCCCGACGGG AGAGATTGTG GTGCGACTGG ACCTGCAGCT CTTCGATGAA 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170971.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171028.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

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CloneID	ODa06106
Clone ID Related Accession (Same CDS sequence)	XM_005170971.4 , XM_005170971.3
Accession Version	XM_005170971.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170971.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG 
CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG 
AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT 
GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG 
TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG 
CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG 
CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC 
CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA 
AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG 
GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC 
GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG 
TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC 
AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA 
CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT 
CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT 
ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT 
ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT 
GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG 
AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC 
TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC 
GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC 
ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC 
CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC 
TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG 
GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171028.1
CDS	227..5482
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_005170971.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170971.4

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG 
CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG 
AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT 
GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG 
TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG 
CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG 
CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC 
CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA 
AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG 
GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC 
GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG 
TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC 
AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA 
CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT 
CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT 
ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT 
ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT 
GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG 
AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC 
TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC 
GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC 
ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC 
CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC 
TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG 
GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170971.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171028.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06106
Clone ID Related Accession (Same CDS sequence)	XM_005170971.4 , XM_005170971.3
Accession Version	XM_005170971.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	intersectin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG 
CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG 
AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT 
GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG 
TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG 
CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG 
CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC 
CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA 
AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG 
GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC 
GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG 
TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC 
AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA 
CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT 
CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT 
ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT 
ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT 
GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG 
AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC 
TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC 
GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC 
ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC 
CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC 
TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG 
GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171028.1
CDS	123..5378
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_005170971.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170971.3

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG 
CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG 
AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT 
GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG 
TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG 
CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG 
CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC 
CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA 
AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG 
GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC 
GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG 
TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC 
AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA 
CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT 
CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT 
ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT 
ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT 
GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG 
AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC 
TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC 
GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC 
ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC 
CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC 
TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG 
GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170972.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171029.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

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CloneID	ODa06107
Clone ID Related Accession (Same CDS sequence)	XM_005170972.3 , XM_005170972.4
Accession Version	XM_005170972.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	intersectin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG 
ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC 
AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT 
TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC 
ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA 
CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC 
ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG 
CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC 
TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT 
GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT 
CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG 
TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT 
GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA 
AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC 
AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG 
CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT 
TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT 
AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG 
ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA 
GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG 
GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT 
CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT 
GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT 
TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA 
GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA 
GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG 
CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC 
CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG 
AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG 
CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT 
GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC 
CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC 
TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT 
CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC 
ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG 
CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG 
ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA 
AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC 
AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT 
CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG 
TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA 
GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC 
ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA 
ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC 
GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT 
AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT 
CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG 
CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG 
GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC 
ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG 
CAGCTCTTCG ATGAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171029.1
CDS	123..5363
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_005170972.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170972.3

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG 
ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC 
AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT 
TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC 
ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA 
CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC 
ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG 
CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC 
TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT 
GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT 
CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG 
TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT 
GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA 
AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC 
AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG 
CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT 
TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT 
AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG 
ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA 
GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG 
GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT 
CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT 
GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT 
TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA 
GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA 
GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG 
CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC 
CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG 
AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG 
CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT 
GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC 
CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC 
TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT 
CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC 
ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG 
CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG 
ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA 
AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC 
AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT 
CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG 
TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA 
GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC 
ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA 
ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC 
GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT 
AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT 
CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG 
CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG 
GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC 
ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG 
CAGCTCTTCG ATGAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170972.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171029.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

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CloneID	ODa06107
Clone ID Related Accession (Same CDS sequence)	XM_005170972.3 , XM_005170972.4
Accession Version	XM_005170972.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170972.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG 
ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC 
AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT 
TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC 
ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA 
CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC 
ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG 
CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC 
TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT 
GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT 
CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG 
TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT 
GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA 
AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC 
AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG 
CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT 
TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT 
AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG 
ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA 
GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG 
GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT 
CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT 
GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT 
TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA 
GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA 
GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG 
CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC 
CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG 
AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG 
CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT 
GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC 
CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC 
TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT 
CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC 
ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG 
CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG 
ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA 
AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC 
AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT 
CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG 
TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA 
GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC 
ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA 
ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC 
GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT 
AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT 
CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG 
CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG 
GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC 
ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG 
CAGCTCTTCG ATGAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171029.1
CDS	227..5467
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_005170972.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170972.4

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG 
ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC 
AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT 
TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC 
ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA 
CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC 
ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG 
CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC 
TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT 
GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT 
CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG 
TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT 
GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA 
AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC 
AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG 
CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT 
TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT 
AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG 
ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA 
GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG 
GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT 
CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT 
GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT 
TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA 
GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA 
GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG 
CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC 
CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG 
AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG 
CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT 
GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC 
CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC 
TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT 
CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC 
ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG 
CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG 
ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA 
AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC 
AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT 
CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG 
TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA 
GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC 
ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA 
ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC 
GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT 
AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT 
CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG 
CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG 
GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC 
ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG 
CAGCTCTTCG ATGAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170974.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171031.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

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CloneID	ODa06109
Clone ID Related Accession (Same CDS sequence)	XM_005170974.4 , XM_005170974.3
Accession Version	XM_005170974.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171031.1
CDS	226..3978
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_005170974.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170974.4

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_005170974.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171031.1 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

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CloneID	ODa06109
Clone ID Related Accession (Same CDS sequence)	XM_005170974.4 , XM_005170974.3
Accession Version	XM_005170974.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	intersectin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171031.1
CDS	123..3875
Translation	MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPLPPGVPGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHSTDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQLEWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIETTNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKESTKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSHDRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGTVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_005170974.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170974.3

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC 
ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC 
CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC 
ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC 
CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT 
ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA 
GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA 
TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT 
GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG 
TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG 
TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT 
CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG 
GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT 
ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG 
CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG 
ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG 
GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA 
AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG 
CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG 
GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA 
CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG 
GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG 
AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG 
CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC 
CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT 
GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG 
ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC 
AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG 
CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC 
GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA 
CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA 
CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG 
CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA 
GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA 
CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT 
GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC 
CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG 
GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT 
GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG 
AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT 
TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG 
CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT 
TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT 
GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG 
CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC 
GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA 
GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG 
CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC 
GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC 
AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA 
GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA 
TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC 
AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG 
AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA 
GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG 
CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG 
GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT 
GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG 
CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT 
GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC 
GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG 
CTGACCACCG ACACAGACCC CAGCCAACAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Biochemical and biophysical research communications399(2)307-12(2010 Aug)
Dergai M,Tsyba L,Dergai O,Zlatskii I,Skrypkina I,Kovalenko V,Rynditch A

olig1 Expression identifies developing oligodendrocytes in zebrafish and requires hedgehog and notch signaling.
Developmental dynamics : an official publication of the American Association of Anatomists238(4)887-98(2009 Apr)
Schebesta M,Serluca FC

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itsn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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