dfna5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dfna5b
Entrez Gene ID	335722
Full Name	gasdermin Eb
Synonyms	dfna5,dfna5b,fk59f08,wu:fc41e05,wu:fk59f08,zgc:91916
General protein information	Preferred Names gasdermin Eb Names deafness, autosomal dominant 5 b gasdermin Eb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157961.3	XP_005158018.1	deafness, autosomal dominant 5 b isoform X1
XM_017351320.1	XP_017206809.1	deafness, autosomal dominant 5 b isoform X2
XM_005157961.4	XP_005158018.1	deafness, autosomal dominant 5 b isoform X1
XM_021466634.1	XP_021322309.1	deafness, autosomal dominant 5 b isoform X1
NM_001001947.1	NP_001001947.1	gasdermin Eb
XM_017351320.2	XP_017206809.1	deafness, autosomal dominant 5 b isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DFNA5	NP_004394.1
Mus musculus (house mouse)	Dfna5	NP_061239.1
Gallus gallus (chicken)	DFNA5	NP_001006361.1
Rattus norvegicus (Norway rat)	Dfna5	NP_001178678.1
Pan troglodytes (chimpanzee)	DFNA5	XP_001159685.2
	DFNA5	XP_001096330.1
Bos taurus (cattle)	DFNA5	NP_001180041.1
Danio rerio (zebrafish)	dfna5	NP_001001947.1
Canis lupus familiaris (dog)	DFNA5	XP_853956.2
Xenopus tropicalis (tropical clawed frog)	dfna5	XP_002933445.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Phylogenetic and evolutionary relationships and developmental expression patterns of the zebrafish twist gene family.
Yeo GH, Cheah FS, Winkler C, Jabs EW, Venkatesh B, Chong SS
Development genes and evolution219(6)289-300(2009 Jun)

Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish.
Blasiole B, Canfield VA, Vollrath MA, Huss D, Mohideen MA, Dickman JD, Cheng KC, Fekete DM, Levenson R
Developmental biology294(1)148-60(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dfna5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dfna5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21330	NM_001001947.1 Latest version!	Danio rerio gasdermin Eb (gsdmeb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa21330	XM_005157961.4 Latest version!	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa21330	XM_021466634.1 Latest version!	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa55365	XM_017351320.2 Latest version!	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa21330	XM_005157961.3	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa55365	XM_017351320.1	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dfna5b ( NM_001001947.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001001947.1 Danio rerio gasdermin Eb (gsdmeb), mRNA.

Price & Availability↑

CloneID	ODa21330
Clone ID Related Accession (Same CDS sequence)	XM_005157961.3 , XM_005157961.4 , XM_021466634.1 , NM_001001947.1
Accession Version	NM_001001947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	gasdermin Eb
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074049.1. ##Evidence-Data-START## Transcript exon combination :: BC074049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001947.1
CDS	91..1509
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKELVGLEGQFKVLSKLPSSTRFSLFQQISLLLQNSAVISILGSTLEDLCGGTQPDPSTLAEVPELKVTLELIQKSAGKKPSGQHQKQSTLTATHLLISALDEMSKPALFLLKSCCSYTTLQALLHLVQNMALNEKSSLKDAALDVLADEVVFKNITSLFNSCNVMLLKDDNSLITKISNPEDRLPLMLCIAVKGLASLAPHV

Target ORF information:

RefSeq Version	NM_001001947.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gasdermin Eb (gsdmeb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001947.1

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dfna5b ( XM_005157961.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158018.1 Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21330
Clone ID Related Accession (Same CDS sequence)	XM_005157961.3 , XM_005157961.4 , XM_021466634.1 , NM_001001947.1
Accession Version	XM_005157961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deafness, autosomal dominant 5 b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158018.1
CDS	122..1540
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKELVGLEGQFKVLSKLPSSTRFSLFQQISLLLQNSAVISILGSTLEDLCGGTQPDPSTLAEVPELKVTLELIQKSAGKKPSGQHQKQSTLTATHLLISALDEMSKPALFLLKSCCSYTTLQALLHLVQNMALNEKSSLKDAALDVLADEVVFKNITSLFNSCNVMLLKDDNSLITKISNPEDRLPLMLCIAVKGLASLAPHV

Target ORF information:

RefSeq Version	XM_005157961.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157961.3

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dfna5b ( XM_005157961.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158018.1 Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21330
Clone ID Related Accession (Same CDS sequence)	XM_005157961.3 , XM_005157961.4 , XM_021466634.1 , NM_001001947.1
Accession Version	XM_005157961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deafness, autosomal dominant 5 b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158018.1
CDS	147..1565
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKELVGLEGQFKVLSKLPSSTRFSLFQQISLLLQNSAVISILGSTLEDLCGGTQPDPSTLAEVPELKVTLELIQKSAGKKPSGQHQKQSTLTATHLLISALDEMSKPALFLLKSCCSYTTLQALLHLVQNMALNEKSSLKDAALDVLADEVVFKNITSLFNSCNVMLLKDDNSLITKISNPEDRLPLMLCIAVKGLASLAPHV

Target ORF information:

RefSeq Version	XM_005157961.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157961.4

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dfna5b ( XM_021466634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322309.1 Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21330
Clone ID Related Accession (Same CDS sequence)	XM_005157961.3 , XM_005157961.4 , XM_021466634.1 , NM_001001947.1
Accession Version	XM_021466634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deafness, autosomal dominant 5 b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322309.1
CDS	284..1702
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKELVGLEGQFKVLSKLPSSTRFSLFQQISLLLQNSAVISILGSTLEDLCGGTQPDPSTLAEVPELKVTLELIQKSAGKKPSGQHQKQSTLTATHLLISALDEMSKPALFLLKSCCSYTTLQALLHLVQNMALNEKSSLKDAALDVLADEVVFKNITSLFNSCNVMLLKDDNSLITKISNPEDRLPLMLCIAVKGLASLAPHV

Target ORF information:

RefSeq Version	XM_021466634.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466634.1

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGAACTTG TAGGACTCGA AGGCCAGTTT 
AAAGTTCTGT CTAAACTGCC GTCTAGCACA CGCTTCTCGT TATTCCAGCA GATCTCTCTA 
CTCCTGCAGA ACAGCGCAGT CATTAGCATT TTGGGGAGCA CACTAGAGGA CCTGTGTGGA 
GGGACACAGC CTGATCCCAG TACATTAGCC GAGGTGCCTG AACTGAAGGT TACACTGGAG 
CTCATACAGA AAAGTGCTGG TAAAAAACCC TCTGGACAAC ATCAGAAGCA AAGCACACTT 
ACTGCCACAC ACCTGCTCAT CAGTGCTCTA GACGAGATGA GTAAGCCAGC ACTCTTCCTG 
CTCAAATCCT GCTGCAGTTA CACAACCCTG CAAGCTCTGC TCCATCTTGT GCAGAATATG 
GCTTTAAATG AGAAATCCTC TCTGAAAGAC GCTGCTCTGG ATGTTTTAGC CGATGAGGTG 
GTTTTCAAAA ACATTACATC ACTCTTTAAT TCCTGCAACG TGATGCTGCT CAAGGATGAC 
AATTCACTTA TTACAAAAAT CAGCAACCCA GAAGATCGTC TTCCCCTCAT GTTGTGCATT 
GCAGTCAAGG GTTTGGCTTC TCTTGCACCC CATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dfna5b ( XM_017351320.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206809.1 Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55365
Clone ID Related Accession (Same CDS sequence)	XM_017351320.1 , XM_017351320.2
Accession Version	XM_017351320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deafness, autosomal dominant 5 b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGCTAGAG GACCTGTGTG GAGGGACACA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206809.1
CDS	131..976
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKARGPVWRDTA

Target ORF information:

RefSeq Version	XM_017351320.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351320.1

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGCTAGAG GACCTGTGTG GAGGGACACA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dfna5b ( XM_017351320.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206809.1 Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55365
Clone ID Related Accession (Same CDS sequence)	XM_017351320.1 , XM_017351320.2
Accession Version	XM_017351320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deafness, autosomal dominant 5 b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGCTAGAG GACCTGTGTG GAGGGACACA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206809.1
CDS	136..981
Translation	MFAKATKNLLSEIDSEGFLIPVLCLNDSDGLSPQALVIKRNRYWFWQQPKYKPTDFKLSDVLVGDPINPVVVETEFLTYKGKVMDTKSGSAVAELGPGTINIGGSGSSKLQSSFGNLKKQELDLQKLLHDSKSRVLDMQHSLIQQTRNAKTEVLAVVKERIITTQPCTITEEVQEGGSCTGMFGFNKTIKVSSNDKGKPSIAYDTDVSIDIPPKTTLAYSVIELDIAHTGHYELCLLPAVKGGFEIDGPVKVKQAVTVSAAPGNPTHNMQKARGPVWRDTA

Target ORF information:

RefSeq Version	XM_017351320.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deafness, autosomal dominant 5 b (dfna5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351320.2

ORF Insert Sequence:

ATGTTTGCAA AAGCCACTAA GAACCTGCTC TCTGAGATAG ACTCTGAAGG TTTCCTGATC 
CCAGTGTTGT GCCTGAATGA CTCAGATGGT CTTAGTCCTC AGGCGCTGGT CATCAAGCGT 
AACCGATACT GGTTTTGGCA GCAACCCAAA TACAAGCCAA CAGACTTCAA ACTGAGTGAT 
GTTCTAGTTG GAGATCCCAT AAATCCAGTT GTGGTTGAAA CTGAATTTTT GACCTATAAA 
GGCAAAGTTA TGGATACTAA AAGTGGAAGT GCTGTAGCAG AACTTGGGCC AGGGACCATT 
AATATAGGTG GCTCCGGATC CAGCAAACTG CAGTCATCAT TTGGAAACTT AAAGAAACAG 
GAGCTGGATT TGCAGAAGCT CTTACATGAT TCCAAATCCA GAGTTCTAGA CATGCAGCAC 
TCTCTGATCC AGCAGACACG GAACGCAAAG ACGGAGGTGT TGGCGGTAGT GAAGGAACGA 
ATCATTACCA CTCAGCCCTG CACCATCACT GAGGAGGTTC AGGAAGGTGG TTCCTGCACT 
GGAATGTTTG GGTTCAATAA GACAATAAAG GTTTCATCTA ATGATAAAGG AAAGCCTTCA 
ATTGCGTACG ACACTGATGT GTCTATCGAC ATCCCCCCCA AAACCACCCT TGCTTACAGC 
GTGATTGAAT TAGACATTGC TCACACAGGA CACTATGAAC TGTGTCTGCT GCCAGCAGTC 
AAAGGTGGAT TTGAAATTGA TGGCCCAGTC AAGGTGAAAC AAGCAGTCAC AGTTAGTGCT 
GCACCAGGGA ATCCAACCCA CAACATGCAG AAAGCTAGAG GACCTGTGTG GAGGGACACA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Phylogenetic and evolutionary relationships and developmental expression patterns of the zebrafish twist gene family.
Development genes and evolution219(6)289-300(2009 Jun)
Yeo GH,Cheah FS,Winkler C,Jabs EW,Venkatesh B,Chong SS

Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish.
Developmental biology294(1)148-60(2006 Jun)
Blasiole B,Canfield VA,Vollrath MA,Huss D,Mohideen MA,Dickman JD,Cheng KC,Fekete DM,Levenson R

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dfna5b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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