cap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cap1
Entrez Gene ID	334527
Full Name	CAP, adenylate cyclase-associated protein 1 (yeast)
Synonyms	fj98f01,wu:fj98f01,zgc:63872
General protein information	Preferred Names CAP, adenylate cyclase-associated protein 1 (yeast) Names adenylyl cyclase-associated protein 1 Cap1 CAP, adenylate cyclase-associated protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199909.2	NP_956203.1	adenylyl cyclase-associated protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CAP1	NP_001030182.1
Drosophila melanogaster (fruit fly)	capt	NP_001033870.1
Rattus norvegicus (Norway rat)	Cap1	NP_071778.2
Gallus gallus (chicken)	CAP1	XP_003642632.1
Danio rerio (zebrafish)	cap1	NP_956203.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010175	XP_319349.4
Caenorhabditis elegans (roundworm)	cas-1	NP_510713.1
Caenorhabditis elegans (roundworm)	cas-2	NP_491324.1
Saccharomyces cerevisiae (baker's yeast)	SRV2	NP_014261.1
Xenopus tropicalis (tropical clawed frog)	cap1	NP_001001239.1
Homo sapiens (human)	CAP1	NP_001099000.1
Canis lupus familiaris (dog)	CAP1	XP_005628951.1
Mus musculus (house mouse)	Cap1	NP_031624.2
Kluyveromyces lactis	KLLA0A11638g	XP_451506.1
Schizosaccharomyces pombe (fission yeast)	cap1	NP_587817.1
Magnaporthe oryzae (rice blast fungus)	MGG_01722	XP_003714719.1
Oryza sativa (rice)	Os08g0447900	NP_001061934.1
Pan troglodytes (chimpanzee)	CAP1	NP_001233522.1
	CAP1	XP_001082428.1
Neurospora crassa	NCU08008	XP_962678.2
Arabidopsis thaliana (thale cress)	CAP1	NP_195175.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Proteome mapping of adult zebrafish marrow neutrophils reveals partial cross species conservation to human peripheral neutrophils.
Singh SK, Sethi S, Aravamudhan S, Kr?ger M, Grabher C
PloS one8(9)e73998(2013)

Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2 in zebrafish body development and cancer progression.
Effendi K, Yamazaki K, Mori T, Masugi Y, Makino S, Sakamoto M
Experimental cell research319(1)35-44(2013 Jan)

Proteomic profile of zebrafish brain based on two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization MS/MS analysis.
Singh SK, Sundaram CS, Shanbhag S, Idris MM
Zebrafish7(2)169-77(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The requirement of Cap1 for a cellular apical constriction step suggests a conservation of gene function for a cell biological event critical to many developmental processes.
Title: Control of morphogenetic cell movements in the early zebrafish myotome.

cap1 regulates cortical actin distribution specifically in the anterior mesendoderm of the zebrafish gastrula.
Title: Developmentally restricted actin-regulatory molecules control morphogenetic cell movements in the zebrafish gastrula.

The following cap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03850	NM_199909.2 Latest version!	Danio rerio CAP, adenylate cyclase-associated protein 1 (yeast) (cap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cap1 ( NM_199909.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956203.1 Danio rerio CAP, adenylate cyclase-associated protein 1 (yeast) (cap1), mRNA.

Price & Availability↑

CloneID	ODa03850
Clone ID Related Accession (Same CDS sequence)	NM_199909.2
Accession Version	NM_199909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	adenylyl cyclase-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY162326.1. On Jan 23, 2004 this sequence version replaced NM_199909.1. ##Evidence-Data-START## Transcript exon combination :: AY162326.1, BC150356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGATC TGGCGGGTCT AGTGCAGCGT CTGGAGGTGG CTGTGGGCCG TCTGGAGTCC 
ATGTCCAGTT CTGGCAGTGG AGGCGGCCCT GCTGCTGGAT CTGGAGCCGT GGGAGTGTAC 
GTTGAAGCCT ATGACGGCCT GATCTCTGGT CCTTTGGCTC AGTACTTTAC CCTCAGTCAG 
AAGATAGGGG GTGACGTCCT GAAGCATGCT GAGATGATGA AGCAGGCCTT TACCAGTCAA 
CGACAGCTGC TGGCCACTGC TTCCTGCTCT CAGAAACCCT CTGATCCTGT TCTGAATGCT 
CTGCTGGCCC CCGTGTCTAA AGCCATCCAG GATGTTCAGG CGTTTCGAGA GAAGAACCGC 
TCCTCTCCTT TCTTTAACCA CCTCTCTGCC GTCAGCGAAA GTGTTCCTGC TCTCGGCTGG 
GTTGCCATGG CCCCAAAGCC TGGCCCCTAT GTGAAGGAGA TGCAGGACGC AGCCATGTTT 
TACACAAACC GTGTGCTCAA GGATTACAAG GAGAAGGATA AGACTCATGT GGAATGGGTC 
AATGCTTATG TGACCATTTG GACCGAGCTG CAAGCCTACA TCAAGCAGCA CCACACCACT 
GGACTGAGCT GGAGCAAGAC TGGTCCCGTA GCTGCTGCTG CTGGAGGAGG AGCCCCAGCA 
CCGCCTCCTC CAGGACCTCC TCCACCCCCA ATGGACCTAA ACAAGTCATC AGGTGGAGAC 
TCCGAACCGA CCAATCGAAA TGCTCTCTTT GCCTCCATCA ACAAAGGATC TGACATCACT 
AAAGGCCTGA AGCATGTGTC TGATGACCAG AAGACCCATA AGAATCCTGG GCTCAGAGGT 
CAGACAGGGC CTTCAGTGTC CGGTCCTAAA CCCTTCACTC CACGGCCCAC AGCCACCGCC 
AGCCCGGCCC GCTCCCTGCC CCCAGTGCTG GAGCTGGACG GCAAGAAATG GAAAGTGGAA 
AATCAAGAAG GCGCCCAGAA TCTGGTGATC AGCGACACGG AGCTGAAACA GGTGGTTTAT 
GGCTTTAAGT GCAACAACAG CACTTTACAG GTGAAGGGGA AGATCAACTC CATCACTCTA 
GATAACTGCA AGAAGATGGG GCTCGTCTTT GATGATGTTG TGGGAATTGT TGAGGTGATC 
AACTGCAGAG ATGTTAAAAT CCAGGTTATT GGTAAAGTGC CAACCATCTC CATCAACAAG 
ACCGATGGCT GCCACGTGTA CCTGAGCAAA GACTCCCTGT CGTGTGAAAT CGTCAGCGCC 
AAGAGCTCCG AGATGAACGT TCTGGTGCCC AACAAAGATG GAGAATTCAC TGAGATCCCA 
GTCCCTGAAC AGTTCAAAAC CGTATGGGAT GGCAGTAAAC TGGTCACCAC AGCCACCGAG 
ATCGCCGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956203.1
CDS	63..1454
Translation	MADLAGLVQRLEVAVGRLESMSSSGSGGGPAAGSGAVGVYVEAYDGLISGPLAQYFTLSQKIGGDVLKHAEMMKQAFTSQRQLLATASCSQKPSDPVLNALLAPVSKAIQDVQAFREKNRSSPFFNHLSAVSESVPALGWVAMAPKPGPYVKEMQDAAMFYTNRVLKDYKEKDKTHVEWVNAYVTIWTELQAYIKQHHTTGLSWSKTGPVAAAAGGGAPAPPPPGPPPPPMDLNKSSGGDSEPTNRNALFASINKGSDITKGLKHVSDDQKTHKNPGLRGQTGPSVSGPKPFTPRPTATASPARSLPPVLELDGKKWKVENQEGAQNLVISDTELKQVVYGFKCNNSTLQVKGKINSITLDNCKKMGLVFDDVVGIVEVINCRDVKIQVIGKVPTISINKTDGCHVYLSKDSLSCEIVSAKSSEMNVLVPNKDGEFTEIPVPEQFKTVWDGSKLVTTATEIAG

Target ORF information:

RefSeq Version	NM_199909.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CAP, adenylate cyclase-associated protein 1 (yeast) (cap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199909.2

ORF Insert Sequence:

ATGGCAGATC TGGCGGGTCT AGTGCAGCGT CTGGAGGTGG CTGTGGGCCG TCTGGAGTCC 
ATGTCCAGTT CTGGCAGTGG AGGCGGCCCT GCTGCTGGAT CTGGAGCCGT GGGAGTGTAC 
GTTGAAGCCT ATGACGGCCT GATCTCTGGT CCTTTGGCTC AGTACTTTAC CCTCAGTCAG 
AAGATAGGGG GTGACGTCCT GAAGCATGCT GAGATGATGA AGCAGGCCTT TACCAGTCAA 
CGACAGCTGC TGGCCACTGC TTCCTGCTCT CAGAAACCCT CTGATCCTGT TCTGAATGCT 
CTGCTGGCCC CCGTGTCTAA AGCCATCCAG GATGTTCAGG CGTTTCGAGA GAAGAACCGC 
TCCTCTCCTT TCTTTAACCA CCTCTCTGCC GTCAGCGAAA GTGTTCCTGC TCTCGGCTGG 
GTTGCCATGG CCCCAAAGCC TGGCCCCTAT GTGAAGGAGA TGCAGGACGC AGCCATGTTT 
TACACAAACC GTGTGCTCAA GGATTACAAG GAGAAGGATA AGACTCATGT GGAATGGGTC 
AATGCTTATG TGACCATTTG GACCGAGCTG CAAGCCTACA TCAAGCAGCA CCACACCACT 
GGACTGAGCT GGAGCAAGAC TGGTCCCGTA GCTGCTGCTG CTGGAGGAGG AGCCCCAGCA 
CCGCCTCCTC CAGGACCTCC TCCACCCCCA ATGGACCTAA ACAAGTCATC AGGTGGAGAC 
TCCGAACCGA CCAATCGAAA TGCTCTCTTT GCCTCCATCA ACAAAGGATC TGACATCACT 
AAAGGCCTGA AGCATGTGTC TGATGACCAG AAGACCCATA AGAATCCTGG GCTCAGAGGT 
CAGACAGGGC CTTCAGTGTC CGGTCCTAAA CCCTTCACTC CACGGCCCAC AGCCACCGCC 
AGCCCGGCCC GCTCCCTGCC CCCAGTGCTG GAGCTGGACG GCAAGAAATG GAAAGTGGAA 
AATCAAGAAG GCGCCCAGAA TCTGGTGATC AGCGACACGG AGCTGAAACA GGTGGTTTAT 
GGCTTTAAGT GCAACAACAG CACTTTACAG GTGAAGGGGA AGATCAACTC CATCACTCTA 
GATAACTGCA AGAAGATGGG GCTCGTCTTT GATGATGTTG TGGGAATTGT TGAGGTGATC 
AACTGCAGAG ATGTTAAAAT CCAGGTTATT GGTAAAGTGC CAACCATCTC CATCAACAAG 
ACCGATGGCT GCCACGTGTA CCTGAGCAAA GACTCCCTGT CGTGTGAAAT CGTCAGCGCC 
AAGAGCTCCG AGATGAACGT TCTGGTGCCC AACAAAGATG GAGAATTCAC TGAGATCCCA 
GTCCCTGAAC AGTTCAAAAC CGTATGGGAT GGCAGTAAAC TGGTCACCAC AGCCACCGAG 
ATCGCCGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Proteome mapping of adult zebrafish marrow neutrophils reveals partial cross species conservation to human peripheral neutrophils.
PloS one8(9)e73998(2013)
Singh SK,Sethi S,Aravamudhan S,Kr?ger M,Grabher C

Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2 in zebrafish body development and cancer progression.
Experimental cell research319(1)35-44(2013 Jan)
Effendi K,Yamazaki K,Mori T,Masugi Y,Makino S,Sakamoto M

Proteomic profile of zebrafish brain based on two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization MS/MS analysis.
Zebrafish7(2)169-77(2010 Jun)
Singh SK,Sundaram CS,Shanbhag S,Idris MM