brf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	brf1b
Entrez Gene ID	334316
Full Name	BRF1, RNA polymerase III transcription initiation factor b
Synonyms	fi40c10,wu:fi40c10,zgc:63497
General protein information	Preferred Names BRF1, RNA polymerase III transcription initiation factor b Names transcription factor IIIB 90 kDa subunit BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIBb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199889.1	NP_956183.1	transcription factor IIIB 90 kDa subunit

Pathways from BioSystems

Homologies

	BRF1	XP_001098066.2
Bos taurus (cattle)	BRF1	XP_005222395.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004233	XP_313145.5
Caenorhabditis elegans (roundworm)	brf-1	NP_495526.1
Saccharomyces cerevisiae (baker's yeast)	BRF1	NP_011762.1
Kluyveromyces lactis	KLLA0A05434g	XP_451240.1
Eremothecium gossypii	AGOS_AEL213W	NP_984648.1
Schizosaccharomyces pombe (fission yeast)	brf1	NP_596265.2
Xenopus tropicalis (tropical clawed frog)	brf1	NP_001182550.1
Homo sapiens (human)	BRF1	NP_001510.2
Mus musculus (house mouse)	Brf1	NP_082469.2
Gallus gallus (chicken)	BRF1	XP_004936477.1
Drosophila melanogaster (fruit fly)	Brf	NP_650616.2
Canis lupus familiaris (dog)	BRF1	XP_005623949.1
Rattus norvegicus (Norway rat)	Brf1	NP_001100231.1
Danio rerio (zebrafish)	brf1b	NP_956183.1

Gene Ontology
Bibliography

Related articles in PubMed

ZNHIT3 is defective in PEHO syndrome, a severe encephalopathy with cerebellar granule neuron loss.
Anttonen AK, Laari A, Kousi M, Yang YJ, J??skel?inen T, Somer M, Siintola E, Jakkula E, Muona M, Tegelberg S, L?nnqvist T, Pihko H, Valanne L, Paetau A, Lun MP, H?stbacka J, Kopra O, Joensuu T, Katsanis N, Lehtinen MK, Palvimo JJ, Lehesjoki AE
Brain : a journal of neurology140(5)1267-1279(2017 05)

BRF1 mutations alter RNA polymerase III-dependent transcription and cause neurodevelopmental anomalies.
Borck G, H?g F, Dentici ML, Tan PL, Sowada N, Medeira A, Gueneau L, Thiele H, Kousi M, Lepri F, Wenzeck L, Blumenthal I, Radicioni A, Schwarzenberg TL, Mandriani B, Fischetto R, Morris-Rosendahl DJ, Altm?ller J, Reymond A, N?rnberg P, Merla G, Dallapiccola B, Katsanis N, Cramer P, Kubisch C
Genome research25(2)155-66(2015 Feb)

A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis.
Bielczyk-Maczy?ska E, Serbanovic-Canic J, Ferreira L, Soranzo N, Stemple DL, Ouwehand WH, Cvejic A
PLoS genetics10(7)e1004450(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following brf1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03738	NM_199889.1 Latest version!	Danio rerio BRF1 RNA polymerase III transcription initiation factor subunit b (brf1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brf1b ( NM_199889.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956183.1 Danio rerio BRF1 RNA polymerase III transcription initiation factor subunit b (brf1b), mRNA.

Price & Availability↑

CloneID	ODa03738
Clone ID Related Accession (Same CDS sequence)	NM_199889.1
Accession Version	NM_199889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	transcription factor IIIB 90 kDa subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060523.1. ##Evidence-Data-START## Transcript exon combination :: BC060523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTCGCC AGTGCAAGAA CTGTGGTGGA TCGGACATCG ACACAGATCC AGCCCGGGGG 
AGTGCAGTCT GCACAGCCTG TGGTTCGGTT CTGGAGGATA ACATCATTGT GTCTGAGGTT 
CAGTTTGTGG AAAACAGTGG AGGAGTCTCT TCAGCCGTCG GCCAGTTTGT GTCTGCAGAT 
GGCACTTCCA AAGCTCCAGT CTTGGGCAGT GGCTTCCACA CCAGCCTGGG AAAAGAATCA 
CGAGCTCAGA CTCTCCAGAA TGGGAAACGG CAGATCCACA ACCTCGGCAG TCAACTTCAG 
CTCAACCAGC ACTGCCTGGA CACGGCCTTC AATTTCTTCA AGATGGTGGT GAGCAAACAC 
TTGACGCGAG GTCGAAAGAT GACACACGTG ATCGCTGCCT GTCTCTACCT GGTGTGCAGG 
ACAGAAGGAA CGCCACACAT GCTCCTGGAC CTGAGTGATC TTTTACAGGT TAATGTTTAC 
ATTCTGGGCA AGACCTTTCT CCTGTTGGCA AGAGAGCTCT GCATCAATGC ACCGGCTGTC 
GATCCATGTC TGTACATTCC ACGATTCGCC CACATGTTGG AGTTTGGTGA AAAGACCCAT 
GAGGTTTCGA TGACTGCTCT CAGACTGCTG CAGAGGATGA AAAGAGATTG GATGCACACA 
GGACGGCGAC CATCAGGATT ATGTGGTGCA GCTCTGCTCG TGGCGGCTCG CATGCATGAA 
TTCCGGCGCA CGGTGAAGGA GGTCATATCT GTTGTAAAGG TGTGCGAGAC TACACTGAGA 
AAAAGACTGA CAGAGTTTGA GGAGACACCC ACCAGTTCCC TGACAATTGA TGAGTTCATG 
CGAGTAGACC TTGAAAAAGA ATGTGATCCT CCATCTTTTG TTGCAGGACA GAAAAAAGTC 
AAGATGCAGC AGCTGGAGCA GGAGTTGGTC AAGAAACTTG AGGAATATCA AGGTGAGATC 
GACTCATACC GCGATGAGAT TGAGACACAG TTGGCAGGAA GCAGCAGAAC CAAAATGAAA 
GGGATTTATG CCTCCTACGC AAGAGAAGAT GATGACTCTT TGTCTGTGAC TTCTGGATTG 
ACTGGAGATG AAGATGCAGA GGATGAGGAA ATGGAGGCAG CAGCTAAACA TCTTAACCAA 
GAGTTCATAA GTCAGGTGCT TAACCGAAGA GACCAGGGAG GAAAGAAGGA TTTGGATGGA 
AGAAAGAACA CAGTCCCGCT TCCTAATCAG CCCAAACATG TGCCCCTGAC TGCCCTCCTC 
GGCCCTCTGC CCAGTGCTGC CAGCCTTGGT TTAACAGACT CCATTAGACA ATGCATCACA 
GAGACAACAA ATGTGGAAGA AAAATCAGAC AGTGGGGAGC TAGATTTGGA TGGGATTGAC 
GAAGAGGAAA TTGAAAAGTA CATTTTGAAT GAGATCGAGG TCGAGGCGAA GACAGAGCTG 
TGGATGAAAC AGAACGAGGA GTATTTGAGG GAACAGAAAG AAAAAGAGGA GAGAATTGCT 
AAAGAGAAGG AACAGGGAAC CTATAAGGAG AAGCCAAAGA AGCCATCCAA GAAGCGCGAA 
CCAATCCTGG CCAGCACTGC GGGAGAGGCT ATAGAGAAGA TGCTGGAGCA GAAAAAGATC 
TCCAGTAAGA TCAATTATGA TGTATTGCGG GACCTCAACA GCAAGGGCAG CGCGAGCAGC 
AGCACCCAAC AAGCCGAGGA TGTGCCCACC AGTGGCTCCG GACGCAAGAG GTTGACCCGC 
CGCAAGAAGC AGCCAAACAA GAGCAATCTC GGACTTGCCA AACCTGCTAG CCTCATGGGC 
AAGAGGCTAC GGCCCTTAAT TTCCTCCGTT CCAAAGAAGA AGAGGACAGC AGCTGCACTG 
TTCACTCCCA GCATGACCAT GATGTTGCCT CCTGCGGCCC CAGAGCCACC TCCGGCCCCC 
ACCCCTCCCC CTGTGCTGGA AAGCGGGCCC GTGGCTTATG ATGAACCAGC CGAGGAAGAG 
GAGGAAATCG AGGAAGAGGC GGAGGAGTCT TGTGTTAGCG CCATGGAGCT GATCGGTGGA 
AATGAATATG GGTGTGAAGC TGTAGAAGAG GAGGTTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956183.1
CDS	232..2313
Translation	MSRQCKNCGGSDIDTDPARGSAVCTACGSVLEDNIIVSEVQFVENSGGVSSAVGQFVSADGTSKAPVLGSGFHTSLGKESRAQTLQNGKRQIHNLGSQLQLNQHCLDTAFNFFKMVVSKHLTRGRKMTHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYILGKTFLLLARELCINAPAVDPCLYIPRFAHMLEFGEKTHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHEFRRTVKEVISVVKVCETTLRKRLTEFEETPTSSLTIDEFMRVDLEKECDPPSFVAGQKKVKMQQLEQELVKKLEEYQGEIDSYRDEIETQLAGSSRTKMKGIYASYAREDDDSLSVTSGLTGDEDAEDEEMEAAAKHLNQEFISQVLNRRDQGGKKDLDGRKNTVPLPNQPKHVPLTALLGPLPSAASLGLTDSIRQCITETTNVEEKSDSGELDLDGIDEEEIEKYILNEIEVEAKTELWMKQNEEYLREQKEKEERIAKEKEQGTYKEKPKKPSKKREPILASTAGEAIEKMLEQKKISSKINYDVLRDLNSKGSASSSTQQAEDVPTSGSGRKRLTRRKKQPNKSNLGLAKPASLMGKRLRPLISSVPKKKRTAAALFTPSMTMMLPPAAPEPPPAPTPPPVLESGPVAYDEPAEEEEEIEEEAEESCVSAMELIGGNEYGCEAVEEEVF

Target ORF information:

RefSeq Version	NM_199889.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRF1 RNA polymerase III transcription initiation factor subunit b (brf1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199889.1

ORF Insert Sequence:

ATGAGTCGCC AGTGCAAGAA CTGTGGTGGA TCGGACATCG ACACAGATCC AGCCCGGGGG 
AGTGCAGTCT GCACAGCCTG TGGTTCGGTT CTGGAGGATA ACATCATTGT GTCTGAGGTT 
CAGTTTGTGG AAAACAGTGG AGGAGTCTCT TCAGCCGTCG GCCAGTTTGT GTCTGCAGAT 
GGCACTTCCA AAGCTCCAGT CTTGGGCAGT GGCTTCCACA CCAGCCTGGG AAAAGAATCA 
CGAGCTCAGA CTCTCCAGAA TGGGAAACGG CAGATCCACA ACCTCGGCAG TCAACTTCAG 
CTCAACCAGC ACTGCCTGGA CACGGCCTTC AATTTCTTCA AGATGGTGGT GAGCAAACAC 
TTGACGCGAG GTCGAAAGAT GACACACGTG ATCGCTGCCT GTCTCTACCT GGTGTGCAGG 
ACAGAAGGAA CGCCACACAT GCTCCTGGAC CTGAGTGATC TTTTACAGGT TAATGTTTAC 
ATTCTGGGCA AGACCTTTCT CCTGTTGGCA AGAGAGCTCT GCATCAATGC ACCGGCTGTC 
GATCCATGTC TGTACATTCC ACGATTCGCC CACATGTTGG AGTTTGGTGA AAAGACCCAT 
GAGGTTTCGA TGACTGCTCT CAGACTGCTG CAGAGGATGA AAAGAGATTG GATGCACACA 
GGACGGCGAC CATCAGGATT ATGTGGTGCA GCTCTGCTCG TGGCGGCTCG CATGCATGAA 
TTCCGGCGCA CGGTGAAGGA GGTCATATCT GTTGTAAAGG TGTGCGAGAC TACACTGAGA 
AAAAGACTGA CAGAGTTTGA GGAGACACCC ACCAGTTCCC TGACAATTGA TGAGTTCATG 
CGAGTAGACC TTGAAAAAGA ATGTGATCCT CCATCTTTTG TTGCAGGACA GAAAAAAGTC 
AAGATGCAGC AGCTGGAGCA GGAGTTGGTC AAGAAACTTG AGGAATATCA AGGTGAGATC 
GACTCATACC GCGATGAGAT TGAGACACAG TTGGCAGGAA GCAGCAGAAC CAAAATGAAA 
GGGATTTATG CCTCCTACGC AAGAGAAGAT GATGACTCTT TGTCTGTGAC TTCTGGATTG 
ACTGGAGATG AAGATGCAGA GGATGAGGAA ATGGAGGCAG CAGCTAAACA TCTTAACCAA 
GAGTTCATAA GTCAGGTGCT TAACCGAAGA GACCAGGGAG GAAAGAAGGA TTTGGATGGA 
AGAAAGAACA CAGTCCCGCT TCCTAATCAG CCCAAACATG TGCCCCTGAC TGCCCTCCTC 
GGCCCTCTGC CCAGTGCTGC CAGCCTTGGT TTAACAGACT CCATTAGACA ATGCATCACA 
GAGACAACAA ATGTGGAAGA AAAATCAGAC AGTGGGGAGC TAGATTTGGA TGGGATTGAC 
GAAGAGGAAA TTGAAAAGTA CATTTTGAAT GAGATCGAGG TCGAGGCGAA GACAGAGCTG 
TGGATGAAAC AGAACGAGGA GTATTTGAGG GAACAGAAAG AAAAAGAGGA GAGAATTGCT 
AAAGAGAAGG AACAGGGAAC CTATAAGGAG AAGCCAAAGA AGCCATCCAA GAAGCGCGAA 
CCAATCCTGG CCAGCACTGC GGGAGAGGCT ATAGAGAAGA TGCTGGAGCA GAAAAAGATC 
TCCAGTAAGA TCAATTATGA TGTATTGCGG GACCTCAACA GCAAGGGCAG CGCGAGCAGC 
AGCACCCAAC AAGCCGAGGA TGTGCCCACC AGTGGCTCCG GACGCAAGAG GTTGACCCGC 
CGCAAGAAGC AGCCAAACAA GAGCAATCTC GGACTTGCCA AACCTGCTAG CCTCATGGGC 
AAGAGGCTAC GGCCCTTAAT TTCCTCCGTT CCAAAGAAGA AGAGGACAGC AGCTGCACTG 
TTCACTCCCA GCATGACCAT GATGTTGCCT CCTGCGGCCC CAGAGCCACC TCCGGCCCCC 
ACCCCTCCCC CTGTGCTGGA AAGCGGGCCC GTGGCTTATG ATGAACCAGC CGAGGAAGAG 
GAGGAAATCG AGGAAGAGGC GGAGGAGTCT TGTGTTAGCG CCATGGAGCT GATCGGTGGA 
AATGAATATG GGTGTGAAGC TGTAGAAGAG GAGGTTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZNHIT3 is defective in PEHO syndrome, a severe encephalopathy with cerebellar granule neuron loss.
Brain : a journal of neurology140(5)1267-1279(2017 05)
Anttonen AK,Laari A,Kousi M,Yang YJ,J??skel?inen T,Somer M,Siintola E,Jakkula E,Muona M,Tegelberg S,L?nnqvist T,Pihko H,Valanne L,Paetau A,Lun MP,H?stbacka J,Kopra O,Joensuu T,Katsanis N,Lehtinen MK,Palvimo JJ,Lehesjoki AE

BRF1 mutations alter RNA polymerase III-dependent transcription and cause neurodevelopmental anomalies.
Genome research25(2)155-66(2015 Feb)
Borck G,H?g F,Dentici ML,Tan PL,Sowada N,Medeira A,Gueneau L,Thiele H,Kousi M,Lepri F,Wenzeck L,Blumenthal I,Radicioni A,Schwarzenberg TL,Mandriani B,Fischetto R,Morris-Rosendahl DJ,Altm?ller J,Reymond A,N?rnberg P,Merla G,Dallapiccola B,Katsanis N,Cramer P,Kubisch C

A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis.
PLoS genetics10(7)e1004450(2014 Jul)
Bielczyk-Maczy?ska E,Serbanovic-Canic J,Ferreira L,Soranzo N,Stemple DL,Ouwehand WH,Cvejic A

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brf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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