cnn3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cnn3a
Entrez Gene ID	326931
Full Name	calponin 3, acidic a
Synonyms	cnn3,wu:fe49h10,zgc:64211
General protein information	Preferred Names calponin 3, acidic a Names calponin-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199753.1	NP_956047.1	calponin-3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CNN3	XP_537063.4
Bos taurus (cattle)	CNN3	NP_001033268.1
Pan troglodytes (chimpanzee)	CNN3	XP_001156968.1
Rattus norvegicus (Norway rat)	Cnn3	NP_062232.1
Danio rerio (zebrafish)	cnn3b	NP_001019244.1
Drosophila melanogaster (fruit fly)	Chd64	NP_647860.1
	CNN3	XP_001103736.1
Gallus gallus (chicken)	CNN3	XP_422326.3
Xenopus tropicalis (tropical clawed frog)	cnn3	NP_989257.1
Homo sapiens (human)	CNN3	NP_001830.1
Mus musculus (house mouse)	Cnn3	NP_082320.1
Danio rerio (zebrafish)	cnn3a	NP_956047.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001313	XP_321834.4

Gene Ontology
Bibliography

Related articles in PubMed

Multiple bioanalytical method to reveal developmental biological responses in zebrafish embryos exposed to triclocarban.
Dong X, Xu H, Wu X, Yang L
Chemosphere193251-258(2018 Feb)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Evolution of the vertebrate paralemmin gene family: ancient origin of gene duplicates suggests distinct functions.
Hultqvist G, Ocampo Daza D, Larhammar D, Kilimann MW
PloS one7(7)e41850(2012)

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ, Giraldez AJ
Genes & development23(5)619-32(2009 Mar)

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Hoffmann JL, Thomason RG, Lee DM, Brill JL, Price BB, Carr GJ, Versteeg DJ
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cnn3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cnn3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32069	NM_199753.1 Latest version!	Danio rerio calponin 3, acidic a (cnn3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cnn3a ( NM_199753.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956047.1 Danio rerio calponin 3, acidic a (cnn3a), mRNA.

Price & Availability↑

CloneID	ODa32069
Clone ID Related Accession (Same CDS sequence)	NM_199753.1
Accession Version	NM_199753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Danio rerio(zebrafish)
Product	calponin-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053309.1. ##Evidence-Data-START## Transcript exon combination :: BC053309.1, EH998541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476730 [ECO:0000348] ##Evidence-Data-END##

ATGACCCAGT TTAACAAGGG ACCTGCCTAC GGATTATCCG CGGAGGTGAA AAACAAGATT 
GCACAGAAAT ATGACCTACA GAAGGAAGAG GAACTTCGTT TCTGGATTGA GGAGGTGACC 
GGTATGCCGA TCGGAGATCA CTTCCAGAAA GGGCTCAAGG ATGGCGTCAT ACTGTGCGAA 
TTGATAAACA AGCTTCAGCC TGGATCTATA AAGAAAATAA ACCATTCACA GTTAAACTGG 
CACAAGTTAG AGAATCTTGG GAATTTCATC AAAGCCATTC TAGCCTACGG GCTGAAGCCC 
AATGACATCT TTGAAGCCAA TGACCTCTTC GAAAACGGGA ACATGACACA AGTTCAGACC 
ACACTTCTCG CTTTGGCCAG TATGGCAAAA ACCAAAGGCA TGGAAACTAA CATTGACATT 
GGTGTAAAAT ACGCAGACAA GCAGCAAAGA AATTTTGATG ATGAGAAGAT GAAGGCTGGC 
CAGTGTGTCA TCGGACTTCA GATGGGTACC AACAAATGCG CAAGTCAGGC TGGAATGACG 
GCCTACGGGA CCAGGAGACA TCTTTACGAC CCCAAAACAC AAACAGAGAA ACCCTATGAC 
CAGACCACAA TCAGCCTGCA AATGGGCACC AACAAAGGTG CAAGCCAGGC TGGTATGTCC 
GCTCCAGGCA CCAGAAGGGA CATCTATGAC CAGAAGGCAG CTCTGCAACC AATGGACAAC 
TCCACCATTT CTTTGCAAAT GGGAACCAAT AAGGTGGCCT CTCAGAAAGG CATGAGCGTG 
TACGGGTTGG GACGGCAGGT TTACGACCCC AAGTATTGCG CCACTCCAAC AGAGCCTATG 
ATCCACGCCA ATGGCAGCCA GGGAACAGGC ACAAACGGCT CAGAAATCAG TGATAGTGAC 
TATCAGGCGG AATACCAGGA GGAGGAGTAC CAGGGCGAAT ACCATGATGA ATACAGAGGG 
CACTATAACG ACCAGGGCAT CGACTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956047.1
CDS	132..1121
Translation	MTQFNKGPAYGLSAEVKNKIAQKYDLQKEEELRFWIEEVTGMPIGDHFQKGLKDGVILCELINKLQPGSIKKINHSQLNWHKLENLGNFIKAILAYGLKPNDIFEANDLFENGNMTQVQTTLLALASMAKTKGMETNIDIGVKYADKQQRNFDDEKMKAGQCVIGLQMGTNKCASQAGMTAYGTRRHLYDPKTQTEKPYDQTTISLQMGTNKGASQAGMSAPGTRRDIYDQKAALQPMDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCATPTEPMIHANGSQGTGTNGSEISDSDYQAEYQEEEYQGEYHDEYRGHYNDQGIDY

Target ORF information:

RefSeq Version	NM_199753.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calponin 3, acidic a (cnn3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199753.1

ORF Insert Sequence:

ATGACCCAGT TTAACAAGGG ACCTGCCTAC GGATTATCCG CGGAGGTGAA AAACAAGATT 
GCACAGAAAT ATGACCTACA GAAGGAAGAG GAACTTCGTT TCTGGATTGA GGAGGTGACC 
GGTATGCCGA TCGGAGATCA CTTCCAGAAA GGGCTCAAGG ATGGCGTCAT ACTGTGCGAA 
TTGATAAACA AGCTTCAGCC TGGATCTATA AAGAAAATAA ACCATTCACA GTTAAACTGG 
CACAAGTTAG AGAATCTTGG GAATTTCATC AAAGCCATTC TAGCCTACGG GCTGAAGCCC 
AATGACATCT TTGAAGCCAA TGACCTCTTC GAAAACGGGA ACATGACACA AGTTCAGACC 
ACACTTCTCG CTTTGGCCAG TATGGCAAAA ACCAAAGGCA TGGAAACTAA CATTGACATT 
GGTGTAAAAT ACGCAGACAA GCAGCAAAGA AATTTTGATG ATGAGAAGAT GAAGGCTGGC 
CAGTGTGTCA TCGGACTTCA GATGGGTACC AACAAATGCG CAAGTCAGGC TGGAATGACG 
GCCTACGGGA CCAGGAGACA TCTTTACGAC CCCAAAACAC AAACAGAGAA ACCCTATGAC 
CAGACCACAA TCAGCCTGCA AATGGGCACC AACAAAGGTG CAAGCCAGGC TGGTATGTCC 
GCTCCAGGCA CCAGAAGGGA CATCTATGAC CAGAAGGCAG CTCTGCAACC AATGGACAAC 
TCCACCATTT CTTTGCAAAT GGGAACCAAT AAGGTGGCCT CTCAGAAAGG CATGAGCGTG 
TACGGGTTGG GACGGCAGGT TTACGACCCC AAGTATTGCG CCACTCCAAC AGAGCCTATG 
ATCCACGCCA ATGGCAGCCA GGGAACAGGC ACAAACGGCT CAGAAATCAG TGATAGTGAC 
TATCAGGCGG AATACCAGGA GGAGGAGTAC CAGGGCGAAT ACCATGATGA ATACAGAGGG 
CACTATAACG ACCAGGGCAT CGACTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple bioanalytical method to reveal developmental biological responses in zebrafish embryos exposed to triclocarban.
Chemosphere193251-258(2018 Feb)
Dong X,Xu H,Wu X,Yang L

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Evolution of the vertebrate paralemmin gene family: ancient origin of gene duplicates suggests distinct functions.
PloS one7(7)e41850(2012)
Hultqvist G,Ocampo Daza D,Larhammar D,Kilimann MW

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Genes & development23(5)619-32(2009 Mar)
Mishima Y,Abreu-Goodger C,Staton AA,Stahlhut C,Shou C,Cheng C,Gerstein M,Enright AJ,Giraldez AJ

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)
Hoffmann JL,Thomason RG,Lee DM,Brill JL,Price BB,Carr GJ,Versteeg DJ