tgm2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tgm2b
Entrez Gene ID	323856
Full Name	transglutaminase 2b
Synonyms	TGM2,fc12b04,wu:fc12b04,zgc:136700
General protein information	Preferred Names transglutaminase 2b Names protein-glutamine gamma-glutamyltransferase 2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212656.2	NP_997821.2	protein-glutamine gamma-glutamyltransferase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TGM2	XP_001144553.2
Canis lupus familiaris (dog)	TGM2	XP_542991.4
Gallus gallus (chicken)	TGM2	NP_990779.1
Danio rerio (zebrafish)	tgm2b	NP_997821.2
Homo sapiens (human)	TGM2	NP_004604.2
Bos taurus (cattle)	TGM2	NP_803473.1
Rattus norvegicus (Norway rat)	Tgm2	NP_062259.2
Xenopus tropicalis (tropical clawed frog)	tgm2	NP_001123852.1
Mus musculus (house mouse)	Tgm2	NP_033399.1

Gene Ontology
Bibliography

Related articles in PubMed

Ikk2 regulates cytokinesis during vertebrate development.
Shen H, Shin EM, Lee S, Mathavan S, Koh H, Osato M, Choi H, Tergaonkar V, Korzh V
Scientific reports7(1)8094(2017 Aug)

Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos.
Rauwerda H, Pagano JF, de Leeuw WC, Ensink W, Nehrdich U, de Jong M, Jonker M, Spaink HP, Breit TM
BMC genomics18(1)287(2017 04)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of the transglutaminase gene family in zebrafish and in vivo analysis of transglutaminase-dependent bone mineralization.
Deasey S, Grichenko O, Du S, Nurminskaya M
Amino acids42(2-3)1065-75(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tgm2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tgm2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30807	NM_212656.2 Latest version!	Danio rerio transglutaminase 2b (tgm2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tgm2b ( NM_212656.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997821.2 Danio rerio transglutaminase 2b (tgm2b), mRNA.

Price & Availability↑

CloneID	ODa30807
Clone ID Related Accession (Same CDS sequence)	NM_212656.2
Accession Version	NM_212656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	protein-glutamine gamma-glutamyltransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC117635.1. On May 25, 2007 this sequence version replaced NM_212656.1. ##Evidence-Data-START## Transcript exon combination :: BC117635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCTGG ACATCGGCTC ATGGGATCTG GCGTGTAAAT TTAATAACAC GGACCACCAC 
ACGGAGCTGA ACGGCACAGA CCGGCTCATC GTGAGGAGAG GACAGGCCTT CACCATCAAC 
CTGCAGCTCA ACTCCGGCTC ATACCAGCCC GGATACAGTC AGATCAACAT CACTGCAGAG 
ACTGGTCCTG ATCCGCAGCA GCAATACGGC ACCAGAGCCG TGTTCAGCCT GAGCTCAGAG 
GTGGACAGTT CATGTTGGAG CGCCGCCGTC TCCAGTCCAC CAGGAGAATC CGTCTGTCTG 
TCCATCTGCG CCGCTCCGGA TGCTCCAATC GGACACTACA CGCTGACTCT AGACGAGCGG 
ATCCAGATCC AATTCATTCT GCTCTTCAAT CCCTGGTGTC CACTTGATGT GGTGTACATG 
GACAATGAGG AGAAGCTGGC GGAGTATGTT TTGGCTCAGG ATGGCATCAT CTTCAGGGGC 
GATGCCGGAT ACCCCGTCCC GCTGGCCTGG AACTTTGGAC AGTTTGAGGA GGGAATCCTG 
GACGCGTGTT TCAGGATCCT CGACATGAAC CCAAAACACA GAAGAAACCC TGCCAAAGAC 
TGTTCTGGAC GCAGAAACGT CATTTATGTC ACCAGAGTGC TGAGTGCTAT GATCAACAGT 
AATGACCAGG ATTCAGGGGT CCTGGAGGGC TGCTGGAGGG ACACATTTGA CGGCGGCGTG 
AGCCCCATGT CCTGGGGAGG AAGCGTTCAG ATACTAAGGA CGTGGGACAG GAGCTCGTGT 
CTGCCAGTGC GGTATGGCCA GTGCTGGGTG TTTGCAGCCG TCGCCTGCAC AGTGGCTCGT 
GCTGTCGGGA TCCCGTGCAG AGTTGTAACA AACTATTATT CAGCCCACGA CACCAACAGC 
AACCTGCTGA TCGAGCGCTA CGTCAACGAG AAAGGAGAAG TTGACCACAG CAGCACCAGA 
GACATGATAT GGAACTATCA CTGTTGGGTC GAGAGCTGGA TGGGTCGTTC CGATCTCCCG 
CCGGGTTTCG ATGGCTGGCA GGCAAGTGAT CCAACGCCGC AGGAGAAAAG TGAAGGTGTG 
TTCTGCTGCG GGCCGGTTCC TGTACGGGCC ATAAAAGAAG GAGAGCTCAC CTTTAAATAC 
GACGCCCCGT TCGTGTTCGC GGAGGTGAAC GCAGATTTAG TGTACTTCCT CAAGTCTAAA 
GACGGGAGCA CAAGAAAGAT CAACTATGAC CAGAAAGTGG GCCAGAAGAT CAGCACCAAG 
AGCGTCGGCC GAGACGAGAG AGAAGACATC ACACACCTCT ACAAATACCC TGAAGGCTCA 
GCGGATGAGA GGCGAGTGTT TGAGAAAGCC AATCATCAGA ATAAGCTGCT CCAGGAGAAG 
CAAAACACGG GTCTGAACAT CACCATCAAA CTGTCCAGCG GCGTCAGGAA GGGCTGCGAC 
TTTGACGTGT TCGCCATCGT GACCAACGGC ACAGCGGAGG AGAAGAAGTG TCGTCTGGTG 
TTCGCGTCCC GAGCGGTGTC GTATAACGGA GTGATCGGCA GAGAGTGCGG CTTTAAAGAC 
TTGCTCAATG TGGAGCTGCC GCCTGGAGGA GAGAGGAAGG TTCCTCTGAG GCTGAACTAC 
AGTAAATACT GCAATAACCT AACAGAAGAT AACCTCATCC GTCTCGGGGC TCTGCTGATC 
GACTACAGCA CCAGAGACGC CATCATGGCC ATGCGGGACA TCGTGCTGGA CGACCCTGAG 
ATCAAGATCA GGATTCTGGG AGAACCCAAG GAGAACCGTA AGCTGGCGGC GGAGCTCACT 
ATCCAGAACC CACTGCCTGA AGCCTTACAG AGCTGCTGCT TCACTATAGA GGGAGCAAAC 
CTGACCGGTG GAGACAGCAT CACACACACA CTGGATTCCA GCATTGAACC GGGGCAGGAG 
GCCAAAGCCA AAATCTACTT CACTCCCACT CAGTCTGGCC TGCGCAAACT CCTCGTGGAC 
TTCAACAGCG ACAAGCTGGG CCACGTTCGA GGATACAGGA ACGTCATCAT CGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997821.2
CDS	35..2074
Translation	MALDIGSWDLACKFNNTDHHTELNGTDRLIVRRGQAFTINLQLNSGSYQPGYSQINITAETGPDPQQQYGTRAVFSLSSEVDSSCWSAAVSSPPGESVCLSICAAPDAPIGHYTLTLDERIQIQFILLFNPWCPLDVVYMDNEEKLAEYVLAQDGIIFRGDAGYPVPLAWNFGQFEEGILDACFRILDMNPKHRRNPAKDCSGRRNVIYVTRVLSAMINSNDQDSGVLEGCWRDTFDGGVSPMSWGGSVQILRTWDRSSCLPVRYGQCWVFAAVACTVARAVGIPCRVVTNYYSAHDTNSNLLIERYVNEKGEVDHSSTRDMIWNYHCWVESWMGRSDLPPGFDGWQASDPTPQEKSEGVFCCGPVPVRAIKEGELTFKYDAPFVFAEVNADLVYFLKSKDGSTRKINYDQKVGQKISTKSVGRDEREDITHLYKYPEGSADERRVFEKANHQNKLLQEKQNTGLNITIKLSSGVRKGCDFDVFAIVTNGTAEEKKCRLVFASRAVSYNGVIGRECGFKDLLNVELPPGGERKVPLRLNYSKYCNNLTEDNLIRLGALLIDYSTRDAIMAMRDIVLDDPEIKIRILGEPKENRKLAAELTIQNPLPEALQSCCFTIEGANLTGGDSITHTLDSSIEPGQEAKAKIYFTPTQSGLRKLLVDFNSDKLGHVRGYRNVIIGK

Target ORF information:

RefSeq Version	NM_212656.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transglutaminase 2b (tgm2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212656.2

ORF Insert Sequence:

ATGGCTCTGG ACATCGGCTC ATGGGATCTG GCGTGTAAAT TTAATAACAC GGACCACCAC 
ACGGAGCTGA ACGGCACAGA CCGGCTCATC GTGAGGAGAG GACAGGCCTT CACCATCAAC 
CTGCAGCTCA ACTCCGGCTC ATACCAGCCC GGATACAGTC AGATCAACAT CACTGCAGAG 
ACTGGTCCTG ATCCGCAGCA GCAATACGGC ACCAGAGCCG TGTTCAGCCT GAGCTCAGAG 
GTGGACAGTT CATGTTGGAG CGCCGCCGTC TCCAGTCCAC CAGGAGAATC CGTCTGTCTG 
TCCATCTGCG CCGCTCCGGA TGCTCCAATC GGACACTACA CGCTGACTCT AGACGAGCGG 
ATCCAGATCC AATTCATTCT GCTCTTCAAT CCCTGGTGTC CACTTGATGT GGTGTACATG 
GACAATGAGG AGAAGCTGGC GGAGTATGTT TTGGCTCAGG ATGGCATCAT CTTCAGGGGC 
GATGCCGGAT ACCCCGTCCC GCTGGCCTGG AACTTTGGAC AGTTTGAGGA GGGAATCCTG 
GACGCGTGTT TCAGGATCCT CGACATGAAC CCAAAACACA GAAGAAACCC TGCCAAAGAC 
TGTTCTGGAC GCAGAAACGT CATTTATGTC ACCAGAGTGC TGAGTGCTAT GATCAACAGT 
AATGACCAGG ATTCAGGGGT CCTGGAGGGC TGCTGGAGGG ACACATTTGA CGGCGGCGTG 
AGCCCCATGT CCTGGGGAGG AAGCGTTCAG ATACTAAGGA CGTGGGACAG GAGCTCGTGT 
CTGCCAGTGC GGTATGGCCA GTGCTGGGTG TTTGCAGCCG TCGCCTGCAC AGTGGCTCGT 
GCTGTCGGGA TCCCGTGCAG AGTTGTAACA AACTATTATT CAGCCCACGA CACCAACAGC 
AACCTGCTGA TCGAGCGCTA CGTCAACGAG AAAGGAGAAG TTGACCACAG CAGCACCAGA 
GACATGATAT GGAACTATCA CTGTTGGGTC GAGAGCTGGA TGGGTCGTTC CGATCTCCCG 
CCGGGTTTCG ATGGCTGGCA GGCAAGTGAT CCAACGCCGC AGGAGAAAAG TGAAGGTGTG 
TTCTGCTGCG GGCCGGTTCC TGTACGGGCC ATAAAAGAAG GAGAGCTCAC CTTTAAATAC 
GACGCCCCGT TCGTGTTCGC GGAGGTGAAC GCAGATTTAG TGTACTTCCT CAAGTCTAAA 
GACGGGAGCA CAAGAAAGAT CAACTATGAC CAGAAAGTGG GCCAGAAGAT CAGCACCAAG 
AGCGTCGGCC GAGACGAGAG AGAAGACATC ACACACCTCT ACAAATACCC TGAAGGCTCA 
GCGGATGAGA GGCGAGTGTT TGAGAAAGCC AATCATCAGA ATAAGCTGCT CCAGGAGAAG 
CAAAACACGG GTCTGAACAT CACCATCAAA CTGTCCAGCG GCGTCAGGAA GGGCTGCGAC 
TTTGACGTGT TCGCCATCGT GACCAACGGC ACAGCGGAGG AGAAGAAGTG TCGTCTGGTG 
TTCGCGTCCC GAGCGGTGTC GTATAACGGA GTGATCGGCA GAGAGTGCGG CTTTAAAGAC 
TTGCTCAATG TGGAGCTGCC GCCTGGAGGA GAGAGGAAGG TTCCTCTGAG GCTGAACTAC 
AGTAAATACT GCAATAACCT AACAGAAGAT AACCTCATCC GTCTCGGGGC TCTGCTGATC 
GACTACAGCA CCAGAGACGC CATCATGGCC ATGCGGGACA TCGTGCTGGA CGACCCTGAG 
ATCAAGATCA GGATTCTGGG AGAACCCAAG GAGAACCGTA AGCTGGCGGC GGAGCTCACT 
ATCCAGAACC CACTGCCTGA AGCCTTACAG AGCTGCTGCT TCACTATAGA GGGAGCAAAC 
CTGACCGGTG GAGACAGCAT CACACACACA CTGGATTCCA GCATTGAACC GGGGCAGGAG 
GCCAAAGCCA AAATCTACTT CACTCCCACT CAGTCTGGCC TGCGCAAACT CCTCGTGGAC 
TTCAACAGCG ACAAGCTGGG CCACGTTCGA GGATACAGGA ACGTCATCAT CGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ikk2 regulates cytokinesis during vertebrate development.
Scientific reports7(1)8094(2017 Aug)
Shen H,Shin EM,Lee S,Mathavan S,Koh H,Osato M,Choi H,Tergaonkar V,Korzh V

Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos.
BMC genomics18(1)287(2017 04)
Rauwerda H,Pagano JF,de Leeuw WC,Ensink W,Nehrdich U,de Jong M,Jonker M,Spaink HP,Breit TM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of the transglutaminase gene family in zebrafish and in vivo analysis of transglutaminase-dependent bone mineralization.
Amino acids42(2-3)1065-75(2012 Feb)
Deasey S,Grichenko O,Du S,Nurminskaya M