cltca cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cltca
Entrez Gene ID	323579
Full Name	clathrin, heavy chain a (Hc)
Synonyms	cb1033,cltc,wu:fc03e11,wu:fc49a11,wu:fd07f02
General protein information	Preferred Names clathrin, heavy chain a (Hc) Names clathrin, heavy polypeptide a clathrin, heavy polypeptide a (Hc)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005391.2	NP_001005391.2	clathrin, heavy polypeptide a
XM_009305357.2	XP_009303632.1	clathrin, heavy polypeptide a isoform X1
XM_009305358.2	XP_009303633.1	clathrin, heavy polypeptide a isoform X2
XM_005172640.4	XP_005172697.1	clathrin, heavy polypeptide a isoform X3
XM_005172640.3	XP_005172697.1	clathrin, heavy polypeptide a isoform X3
XM_009305357.3	XP_009303632.1	clathrin, heavy polypeptide a isoform X1
XM_009305358.3	XP_009303633.1	clathrin, heavy polypeptide a isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis.
Ellis K, Bagwell J, Bagnat M
The Journal of cell biology200(5)667-79(2013 Mar)

Context-dependent proangiogenic function of bone morphogenetic protein signaling is mediated by disabled homolog 2.
Kim JD, Kang H, Larriv?e B, Lee MY, Mettlen M, Schmid SL, Roman BL, Qyang Y, Eichmann A, Jin SW
Developmental cell23(2)441-8(2012 Aug)

A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Trinh le A, Hochgreb T, Graham M, Wu D, Ruf-Zamojski F, Jayasena CS, Saxena A, Hawk R, Gonzalez-Serricchio A, Dixson A, Chow E, Gonzales C, Leung HY, Solomon I, Bronner-Fraser M, Megason SG, Fraser SE
Genes & development25(21)2306-20(2011 Nov)

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ, Giraldez AJ
Genes & development23(5)619-32(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cltca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cltca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02635	NM_001005391.2 Latest version!	Danio rerio clathrin, heavy chain a (Hc) (cltca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa16203	XM_005172640.4 Latest version!	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa39771	XM_009305357.3 Latest version!	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa39772	XM_009305358.3 Latest version!	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa16203	XM_005172640.3	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa39771	XM_009305357.2	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa39772	XM_009305358.2	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cltca ( NM_001005391.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001005391.2 Danio rerio clathrin, heavy chain a (Hc) (cltca), mRNA.

Price & Availability↑

CloneID	ODa02635
Clone ID Related Accession (Same CDS sequence)	NM_001005391.2
Accession Version	NM_001005391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	clathrin, heavy polypeptide a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB769086.1, BC163714.1, AY648777.1 and CR392341.14. On Apr 15, 2009 this sequence version replaced NM_001005391.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY648777.1, BC163714.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGAAGCTAA ACTCACTGAC CAGCTGCCTC TCATTATTGT GTGCGATCGC 
TTCGACTTCG TCCACGACCT TGTTCTTTAC CTGTACAGAA ACAACCTGCA GAAGTACATC 
GAAATCTACG TCCAGAAGGT AAACCCAAGC CGTCTGCCTG TGGTGATTGG AGGTCTGCTG 
GATGTGGACT GCTCTGAGGA TGTCATCAAA AACCTGATCC TGGTTGTACG GGGACAGTTT 
TCCACCGATG AGCTGGTGGC AGAAGTGGAG AAGAGAAACA GACTGAAGCT GTTGTTGCCC 
TGGCTGGAGT CTCGTATCCA CGAGGGCTGT GAGGAGCCAG CTACCCACAA TGCCCTGGCC 
AAGATCTACA TCGACAGCAA CAACAACCCT GAACGCTTCC TGCGGGAGAA CCCATACTAC 
GATAGCCGCG TGGTGGGGAA ATACTGCGAA AAACGAGACC CTCATCTGGC CTGTGTGGCC 
TATGAGAGAG GAACGTGTGA CCAGGAGCTG ATCAACGTTT GCAATGAGAA CTCGCTGTTC 
AAGAGTTTGT CCCGCTATCT GGTGCGCCGC AGGGACCCTG AGCTATGGGC CAGTGTGCTT 
CTGGAGACCA ACCCCTTCAG GAGGCCCCTT ATTGACCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACTCAGG ACCCAGAGGA GGTGTCAGTC ACAGTGAAGG CCTTCATGAC TGCTGACCTG 
CCCAATGAAC TCATTGAGCT GCTGGAAAAG ATTGTCCTGG ACAACTCCGT CTTCAGTGAA 
CACAGAAACC TGCAGAACCT GCTCATCCTA ACAGCCATCA AAGCTGACCG TACTCGGGTC 
ATGGAGTACA TCAACCGCCT GGACAACTAC GATGCCCCAG ACATCGCCAA CATCGCCATC 
AGCAGCGAGC TGTTTGAGGA GGCCTTCGCC ATCTTCAAGA AATTCGATGT CAACACCTCT 
GCAGTGCAGG TTCTGATCGA GCACATTGGT AATCTGGACC GGGCCTATGA GTTTGCGGAG 
CGCTGCAATG AACCTGCGGT GTGGAGTCAA CTGGCCAAGG CTCAGCTTCA GAAGGGTCTC 
GTCAAAGAGT CCATCGACTC CTACATCAAA GCTGATGATC CTTCAGCTTA CATGGAAGTG 
GTCCAAGCTG CTGACCAAAG TGGAAACTGG GAGGACCTGG TGAAGTTTTT GCAGATGGCC 
AGAAAGAAGG CCAGAGAGTC TTACGTGGAG ACTGAGCTGA TCTTCGCTCT GGCCAAAACC 
AACCGTCTCG CTGAACTGGA GGAGTTTATC AATGGACCAA ATAATGCACA CATCCAGCAG 
GTTGGAGATC GCTGCTATGA TGAGAAGATG TACGAGGCGG CCAAACTTCT CTACAATAAC 
GTCTCCAACT TCGGCCGCTT GGCTTCCACT CTGGTCCACT TGGGCGAGTA TCAAGCCGCT 
GTCGATGGTG CCCGCAAGGC CAACAGCACT CGCACATGGA AAGAGGTTTG CTTTGCCTGT 
GTGGATGGAA AGGAGTTCAG ATTGGCTCAA ATGTGCGGCC TTCATATCGT GGTTCACGCA 
GATGAATTGG AAGAGCTCAT CAACTACTAT CAGGACCGTG GCTACTTTGA GGAGCTGATC 
ACCATGCTGG AAGCGGCGCT GGGGTTGGAG CGCGCTCATA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAATT CAAGCCACAG AAGATGAGGG AGCACCTGGA GCTCTTCTGG 
TCCAGAGTCA ACATCCCAAA GGTTTTGAGG GCTGCAGAGC AGGCTCACCT GTGGGCAGAA 
CTGGTTTTCC TGTACGACAA GTATGAAGAG TTTGATAACG CCATCATCAC CATGATGAGC 
CATCCCACAG ACGCCTGGAA AGAGGGACAG TTCAAGGACA TCATCACCAA GGTGGCCAAT 
GTGGAACTGT ACTACAAAGC CATCCAGTTT TATCTGGAAT TCAGGCCCTT GTTACTGAAC 
GACCTGCTCA TAGTTCTTTC TCCAAGACTC GATCACTCGC GAGCCGTCAA CTTCTTCAGC 
AAGGTTAAGC AGCTTCCTCT GGTCAAGCCT TACCTGCGGT CAGTACAAAA CCACAACAAC 
AAGTCAGTCA ATGAAGCACT TAACAACCTC TTCATCTCAG AGGAGGACTA TCAGGCCCTC 
AGGACGTCCA TAGACGCGTA CGATAACTTC GACAACATCT CTCTGGCTCA GCGTCTGGAG 
AAACACGAGT TAATTGAGTT CAGGAGAATC GCTGCTTACC TCTTCAAGGG GAACAACCGC 
TGGAAGCAGA GCGTGGAGCT GTGCAAGAAA GACAAGCTCT ACAAGGATGC CATGCAATAT 
GCATCGGAGT CAAAGGACAC AGAGCTGGCC GAGGAGCTGC TGCAGTGGTT CCTGGATGAG 
AATAAGAAGG AGTGTTTTGC CGCCTGCCTC TTCACCTGCT ACGATCTGCT CCGGCCTGAC 
GTGGTGCTGG AGACATCCTG GAGGAACAAC ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAATACCT CAGCAAGGTG GACAAGCTAG AGACCTCTGA GTCCTTGAGG 
AAAGAGGAGG AGCAAGCGAC AGAAACTCAA CCAATTGTTT ATGGCACACC CCAGCTCATG 
CTGACAGCAG GCCCCAGTGT GCCCGTCCCA CCACAGCAGG GGTATGGCTA TGGTTACACA 
GCAGCTCCTG GTTATCCCCC TCAGGCTCCT CAGGCCCAGC CGGGCTTCGG TTATGGCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005391.2
CDS	278..5320
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	NM_001005391.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005391.2

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGAAGCTAA ACTCACTGAC CAGCTGCCTC TCATTATTGT GTGCGATCGC 
TTCGACTTCG TCCACGACCT TGTTCTTTAC CTGTACAGAA ACAACCTGCA GAAGTACATC 
GAAATCTACG TCCAGAAGGT AAACCCAAGC CGTCTGCCTG TGGTGATTGG AGGTCTGCTG 
GATGTGGACT GCTCTGAGGA TGTCATCAAA AACCTGATCC TGGTTGTACG GGGACAGTTT 
TCCACCGATG AGCTGGTGGC AGAAGTGGAG AAGAGAAACA GACTGAAGCT GTTGTTGCCC 
TGGCTGGAGT CTCGTATCCA CGAGGGCTGT GAGGAGCCAG CTACCCACAA TGCCCTGGCC 
AAGATCTACA TCGACAGCAA CAACAACCCT GAACGCTTCC TGCGGGAGAA CCCATACTAC 
GATAGCCGCG TGGTGGGGAA ATACTGCGAA AAACGAGACC CTCATCTGGC CTGTGTGGCC 
TATGAGAGAG GAACGTGTGA CCAGGAGCTG ATCAACGTTT GCAATGAGAA CTCGCTGTTC 
AAGAGTTTGT CCCGCTATCT GGTGCGCCGC AGGGACCCTG AGCTATGGGC CAGTGTGCTT 
CTGGAGACCA ACCCCTTCAG GAGGCCCCTT ATTGACCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACTCAGG ACCCAGAGGA GGTGTCAGTC ACAGTGAAGG CCTTCATGAC TGCTGACCTG 
CCCAATGAAC TCATTGAGCT GCTGGAAAAG ATTGTCCTGG ACAACTCCGT CTTCAGTGAA 
CACAGAAACC TGCAGAACCT GCTCATCCTA ACAGCCATCA AAGCTGACCG TACTCGGGTC 
ATGGAGTACA TCAACCGCCT GGACAACTAC GATGCCCCAG ACATCGCCAA CATCGCCATC 
AGCAGCGAGC TGTTTGAGGA GGCCTTCGCC ATCTTCAAGA AATTCGATGT CAACACCTCT 
GCAGTGCAGG TTCTGATCGA GCACATTGGT AATCTGGACC GGGCCTATGA GTTTGCGGAG 
CGCTGCAATG AACCTGCGGT GTGGAGTCAA CTGGCCAAGG CTCAGCTTCA GAAGGGTCTC 
GTCAAAGAGT CCATCGACTC CTACATCAAA GCTGATGATC CTTCAGCTTA CATGGAAGTG 
GTCCAAGCTG CTGACCAAAG TGGAAACTGG GAGGACCTGG TGAAGTTTTT GCAGATGGCC 
AGAAAGAAGG CCAGAGAGTC TTACGTGGAG ACTGAGCTGA TCTTCGCTCT GGCCAAAACC 
AACCGTCTCG CTGAACTGGA GGAGTTTATC AATGGACCAA ATAATGCACA CATCCAGCAG 
GTTGGAGATC GCTGCTATGA TGAGAAGATG TACGAGGCGG CCAAACTTCT CTACAATAAC 
GTCTCCAACT TCGGCCGCTT GGCTTCCACT CTGGTCCACT TGGGCGAGTA TCAAGCCGCT 
GTCGATGGTG CCCGCAAGGC CAACAGCACT CGCACATGGA AAGAGGTTTG CTTTGCCTGT 
GTGGATGGAA AGGAGTTCAG ATTGGCTCAA ATGTGCGGCC TTCATATCGT GGTTCACGCA 
GATGAATTGG AAGAGCTCAT CAACTACTAT CAGGACCGTG GCTACTTTGA GGAGCTGATC 
ACCATGCTGG AAGCGGCGCT GGGGTTGGAG CGCGCTCATA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAATT CAAGCCACAG AAGATGAGGG AGCACCTGGA GCTCTTCTGG 
TCCAGAGTCA ACATCCCAAA GGTTTTGAGG GCTGCAGAGC AGGCTCACCT GTGGGCAGAA 
CTGGTTTTCC TGTACGACAA GTATGAAGAG TTTGATAACG CCATCATCAC CATGATGAGC 
CATCCCACAG ACGCCTGGAA AGAGGGACAG TTCAAGGACA TCATCACCAA GGTGGCCAAT 
GTGGAACTGT ACTACAAAGC CATCCAGTTT TATCTGGAAT TCAGGCCCTT GTTACTGAAC 
GACCTGCTCA TAGTTCTTTC TCCAAGACTC GATCACTCGC GAGCCGTCAA CTTCTTCAGC 
AAGGTTAAGC AGCTTCCTCT GGTCAAGCCT TACCTGCGGT CAGTACAAAA CCACAACAAC 
AAGTCAGTCA ATGAAGCACT TAACAACCTC TTCATCTCAG AGGAGGACTA TCAGGCCCTC 
AGGACGTCCA TAGACGCGTA CGATAACTTC GACAACATCT CTCTGGCTCA GCGTCTGGAG 
AAACACGAGT TAATTGAGTT CAGGAGAATC GCTGCTTACC TCTTCAAGGG GAACAACCGC 
TGGAAGCAGA GCGTGGAGCT GTGCAAGAAA GACAAGCTCT ACAAGGATGC CATGCAATAT 
GCATCGGAGT CAAAGGACAC AGAGCTGGCC GAGGAGCTGC TGCAGTGGTT CCTGGATGAG 
AATAAGAAGG AGTGTTTTGC CGCCTGCCTC TTCACCTGCT ACGATCTGCT CCGGCCTGAC 
GTGGTGCTGG AGACATCCTG GAGGAACAAC ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAATACCT CAGCAAGGTG GACAAGCTAG AGACCTCTGA GTCCTTGAGG 
AAAGAGGAGG AGCAAGCGAC AGAAACTCAA CCAATTGTTT ATGGCACACC CCAGCTCATG 
CTGACAGCAG GCCCCAGTGT GCCCGTCCCA CCACAGCAGG GGTATGGCTA TGGTTACACA 
GCAGCTCCTG GTTATCCCCC TCAGGCTCCT CAGGCCCAGC CGGGCTTCGG TTATGGCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_005172640.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172697.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.

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CloneID	ODa16203
Clone ID Related Accession (Same CDS sequence)	XM_005172640.4 , XM_005172640.3
Accession Version	XM_005172640.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	clathrin, heavy polypeptide a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172640.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGAAGC TAAACTCACT GACCAGCTGC CTCTCATTAT TGTGTGCGAT 
CGCTTCGACT TCGTCCACGA CCTTGTTCTT TACCTGTACA GAAACAACCT GCAGAAGTAC 
ATCGAAATCT ACGTCCAGAA GGTAAACCCA AGCCGTCTGC CTGTGGTGAT TGGAGGTCTG 
CTGGATGTGG ACTGCTCTGA GGATGTCATC AAAAACCTGA TCCTGGTTGT ACGGGGACAG 
TTTTCCACCG ATGAGCTGGT GGCAGAAGTG GAGAAGAGAA ACAGACTGAA GCTGTTGTTG 
CCCTGGCTGG AGTCTCGTAT CCACGAGGGC TGTGAGGAGC CAGCTACCCA CAATGCCCTG 
GCCAAGATCT ACATCGACAG CAACAACAAC CCTGAACGCT TCCTGCGGGA GAACCCATAC 
TACGATAGCC GCGTGGTGGG GAAATACTGC GAAAAACGAG ACCCTCATCT GGCCTGTGTG 
GCCTATGAGA GAGGAACGTG TGACCAGGAG CTGATCAACG TTTGCAATGA GAACTCGCTG 
TTCAAGAGTT TGTCCCGCTA TCTGGTGCGC CGCAGGGACC CTGAGCTATG GGCCAGTGTG 
CTTCTGGAGA CCAACCCCTT CAGGAGGCCC CTTATTGACC AGGTGGTGCA GACAGCCCTG 
TCAGAGACTC AGGACCCAGA GGAGGTGTCA GTCACAGTGA AGGCCTTCAT GACTGCTGAC 
CTGCCCAATG AACTCATTGA GCTGCTGGAA AAGATTGTCC TGGACAACTC CGTCTTCAGT 
GAACACAGAA ACCTGCAGAA CCTGCTCATC CTAACAGCCA TCAAAGCTGA CCGTACTCGG 
GTCATGGAGT ACATCAACCG CCTGGACAAC TACGATGCCC CAGACATCGC CAACATCGCC 
ATCAGCAGCG AGCTGTTTGA GGAGGCCTTC GCCATCTTCA AGAAATTCGA TGTCAACACC 
TCTGCAGTGC AGGTTCTGAT CGAGCACATT GGTAATCTGG ACCGGGCCTA TGAGTTTGCG 
GAGCGCTGCA ATGAACCTGC GGTGTGGAGT CAACTGGCCA AGGCTCAGCT TCAGAAGGGT 
CTCGTCAAAG AGTCCATCGA CTCCTACATC AAAGCTGATG ATCCTTCAGC TTACATGGAA 
GTGGTCCAAG CTGCTGACCA AAGTGGAAAC TGGGAGGACC TGGTGAAGTT TTTGCAGATG 
GCCAGAAAGA AGGCCAGAGA GTCTTACGTG GAGACTGAGC TGATCTTCGC TCTGGCCAAA 
ACCAACCGTC TCGCTGAACT GGAGGAGTTT ATCAATGGAC CAAATAATGC ACACATCCAG 
CAGGTTGGAG ATCGCTGCTA TGATGAGAAG ATGTACGAGG CGGCCAAACT TCTCTACAAT 
AACGTCTCCA ACTTCGGCCG CTTGGCTTCC ACTCTGGTCC ACTTGGGCGA GTATCAAGCC 
GCTGTCGATG GTGCCCGCAA GGCCAACAGC ACTCGCACAT GGAAAGAGGT TTGCTTTGCC 
TGTGTGGATG GAAAGGAGTT CAGATTGGCT CAAATGTGCG GCCTTCATAT CGTGGTTCAC 
GCAGATGAAT TGGAAGAGCT CATCAACTAC TATCAGGACC GTGGCTACTT TGAGGAGCTG 
ATCACCATGC TGGAAGCGGC GCTGGGGTTG GAGCGCGCTC ATATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA ATTCAAGCCA CAGAAGATGA GGGAGCACCT GGAGCTCTTC 
TGGTCCAGAG TCAACATCCC AAAGGTTTTG AGGGCTGCAG AGCAGGCTCA CCTGTGGGCA 
GAACTGGTTT TCCTGTACGA CAAGTATGAA GAGTTTGATA ACGCCATCAT CACCATGATG 
AGCCATCCCA CAGACGCCTG GAAAGAGGGA CAGTTCAAGG ACATCATCAC CAAGGTGGCC 
AATGTGGAAC TGTACTACAA AGCCATCCAG TTTTATCTGG AATTCAGGCC CTTGTTACTG 
AACGACCTGC TCATAGTTCT TTCTCCAAGA CTCGATCACT CGCGAGCCGT CAACTTCTTC 
AGCAAGGTTA AGCAGCTTCC TCTGGTCAAG CCTTACCTGC GGTCAGTACA AAACCACAAC 
AACAAGTCAG TCAATGAAGC ACTTAACAAC CTCTTCATCT CAGAGGAGGA CTATCAGGCC 
CTCAGGACGT CCATAGACGC GTACGATAAC TTCGACAACA TCTCTCTGGC TCAGCGTCTG 
GAGAAACACG AGTTAATTGA GTTCAGGAGA ATCGCTGCTT ACCTCTTCAA GGGGAACAAC 
CGCTGGAAGC AGAGCGTGGA GCTGTGCAAG AAAGACAAGC TCTACAAGGA TGCCATGCAA 
TATGCATCGG AGTCAAAGGA CACAGAGCTG GCCGAGGAGC TGCTGCAGTG GTTCCTGGAT 
GAGAATAAGA AGGAGTGTTT TGCCGCCTGC CTCTTCACCT GCTACGATCT GCTCCGGCCT 
GACGTGGTGC TGGAGACATC CTGGAGGAAC AACATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAATA CCTCAGCAAG GTGGACAAGC TAGAGACCTC TGAGTCCTTG 
AGGAAAGAGG AGGAGCAAGC GACAGAAACT CAACCAATTG TTTATGGCAC ACCCCAGCTC 
ATGCTGACAG CAGGCCCCAG TGTGCCCGTC CCACCACAGC AGGGGTATGG CTATGGTTAC 
ACAGCAGCTC CTGGTTATCC CCCTCAGGCT CCTCAGGCCC AGCCGGGCTT CGGTTATGGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172697.1
CDS	268..5313
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_005172640.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172640.4

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGAAGC TAAACTCACT GACCAGCTGC CTCTCATTAT TGTGTGCGAT 
CGCTTCGACT TCGTCCACGA CCTTGTTCTT TACCTGTACA GAAACAACCT GCAGAAGTAC 
ATCGAAATCT ACGTCCAGAA GGTAAACCCA AGCCGTCTGC CTGTGGTGAT TGGAGGTCTG 
CTGGATGTGG ACTGCTCTGA GGATGTCATC AAAAACCTGA TCCTGGTTGT ACGGGGACAG 
TTTTCCACCG ATGAGCTGGT GGCAGAAGTG GAGAAGAGAA ACAGACTGAA GCTGTTGTTG 
CCCTGGCTGG AGTCTCGTAT CCACGAGGGC TGTGAGGAGC CAGCTACCCA CAATGCCCTG 
GCCAAGATCT ACATCGACAG CAACAACAAC CCTGAACGCT TCCTGCGGGA GAACCCATAC 
TACGATAGCC GCGTGGTGGG GAAATACTGC GAAAAACGAG ACCCTCATCT GGCCTGTGTG 
GCCTATGAGA GAGGAACGTG TGACCAGGAG CTGATCAACG TTTGCAATGA GAACTCGCTG 
TTCAAGAGTT TGTCCCGCTA TCTGGTGCGC CGCAGGGACC CTGAGCTATG GGCCAGTGTG 
CTTCTGGAGA CCAACCCCTT CAGGAGGCCC CTTATTGACC AGGTGGTGCA GACAGCCCTG 
TCAGAGACTC AGGACCCAGA GGAGGTGTCA GTCACAGTGA AGGCCTTCAT GACTGCTGAC 
CTGCCCAATG AACTCATTGA GCTGCTGGAA AAGATTGTCC TGGACAACTC CGTCTTCAGT 
GAACACAGAA ACCTGCAGAA CCTGCTCATC CTAACAGCCA TCAAAGCTGA CCGTACTCGG 
GTCATGGAGT ACATCAACCG CCTGGACAAC TACGATGCCC CAGACATCGC CAACATCGCC 
ATCAGCAGCG AGCTGTTTGA GGAGGCCTTC GCCATCTTCA AGAAATTCGA TGTCAACACC 
TCTGCAGTGC AGGTTCTGAT CGAGCACATT GGTAATCTGG ACCGGGCCTA TGAGTTTGCG 
GAGCGCTGCA ATGAACCTGC GGTGTGGAGT CAACTGGCCA AGGCTCAGCT TCAGAAGGGT 
CTCGTCAAAG AGTCCATCGA CTCCTACATC AAAGCTGATG ATCCTTCAGC TTACATGGAA 
GTGGTCCAAG CTGCTGACCA AAGTGGAAAC TGGGAGGACC TGGTGAAGTT TTTGCAGATG 
GCCAGAAAGA AGGCCAGAGA GTCTTACGTG GAGACTGAGC TGATCTTCGC TCTGGCCAAA 
ACCAACCGTC TCGCTGAACT GGAGGAGTTT ATCAATGGAC CAAATAATGC ACACATCCAG 
CAGGTTGGAG ATCGCTGCTA TGATGAGAAG ATGTACGAGG CGGCCAAACT TCTCTACAAT 
AACGTCTCCA ACTTCGGCCG CTTGGCTTCC ACTCTGGTCC ACTTGGGCGA GTATCAAGCC 
GCTGTCGATG GTGCCCGCAA GGCCAACAGC ACTCGCACAT GGAAAGAGGT TTGCTTTGCC 
TGTGTGGATG GAAAGGAGTT CAGATTGGCT CAAATGTGCG GCCTTCATAT CGTGGTTCAC 
GCAGATGAAT TGGAAGAGCT CATCAACTAC TATCAGGACC GTGGCTACTT TGAGGAGCTG 
ATCACCATGC TGGAAGCGGC GCTGGGGTTG GAGCGCGCTC ATATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA ATTCAAGCCA CAGAAGATGA GGGAGCACCT GGAGCTCTTC 
TGGTCCAGAG TCAACATCCC AAAGGTTTTG AGGGCTGCAG AGCAGGCTCA CCTGTGGGCA 
GAACTGGTTT TCCTGTACGA CAAGTATGAA GAGTTTGATA ACGCCATCAT CACCATGATG 
AGCCATCCCA CAGACGCCTG GAAAGAGGGA CAGTTCAAGG ACATCATCAC CAAGGTGGCC 
AATGTGGAAC TGTACTACAA AGCCATCCAG TTTTATCTGG AATTCAGGCC CTTGTTACTG 
AACGACCTGC TCATAGTTCT TTCTCCAAGA CTCGATCACT CGCGAGCCGT CAACTTCTTC 
AGCAAGGTTA AGCAGCTTCC TCTGGTCAAG CCTTACCTGC GGTCAGTACA AAACCACAAC 
AACAAGTCAG TCAATGAAGC ACTTAACAAC CTCTTCATCT CAGAGGAGGA CTATCAGGCC 
CTCAGGACGT CCATAGACGC GTACGATAAC TTCGACAACA TCTCTCTGGC TCAGCGTCTG 
GAGAAACACG AGTTAATTGA GTTCAGGAGA ATCGCTGCTT ACCTCTTCAA GGGGAACAAC 
CGCTGGAAGC AGAGCGTGGA GCTGTGCAAG AAAGACAAGC TCTACAAGGA TGCCATGCAA 
TATGCATCGG AGTCAAAGGA CACAGAGCTG GCCGAGGAGC TGCTGCAGTG GTTCCTGGAT 
GAGAATAAGA AGGAGTGTTT TGCCGCCTGC CTCTTCACCT GCTACGATCT GCTCCGGCCT 
GACGTGGTGC TGGAGACATC CTGGAGGAAC AACATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAATA CCTCAGCAAG GTGGACAAGC TAGAGACCTC TGAGTCCTTG 
AGGAAAGAGG AGGAGCAAGC GACAGAAACT CAACCAATTG TTTATGGCAC ACCCCAGCTC 
ATGCTGACAG CAGGCCCCAG TGTGCCCGTC CCACCACAGC AGGGGTATGG CTATGGTTAC 
ACAGCAGCTC CTGGTTATCC CCCTCAGGCT CCTCAGGCCC AGCCGGGCTT CGGTTATGGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_005172640.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172697.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa16203
Clone ID Related Accession (Same CDS sequence)	XM_005172640.4 , XM_005172640.3
Accession Version	XM_005172640.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	clathrin, heavy polypeptide a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGAAGC TAAACTCACT GACCAGCTGC CTCTCATTAT TGTGTGCGAT 
CGCTTCGACT TCGTCCACGA CCTTGTTCTT TACCTGTACA GAAACAACCT GCAGAAGTAC 
ATCGAAATCT ACGTCCAGAA GGTAAACCCA AGCCGTCTGC CTGTGGTGAT TGGAGGTCTG 
CTGGATGTGG ACTGCTCTGA GGATGTCATC AAAAACCTGA TCCTGGTTGT ACGGGGACAG 
TTTTCCACCG ATGAGCTGGT GGCAGAAGTG GAGAAGAGAA ACAGACTGAA GCTGTTGTTG 
CCCTGGCTGG AGTCTCGTAT CCACGAGGGC TGTGAGGAGC CAGCTACCCA CAATGCCCTG 
GCCAAGATCT ACATCGACAG CAACAACAAC CCTGAACGCT TCCTGCGGGA GAACCCATAC 
TACGATAGCC GCGTGGTGGG GAAATACTGC GAAAAACGAG ACCCTCATCT GGCCTGTGTG 
GCCTATGAGA GAGGAACGTG TGACCAGGAG CTGATCAACG TTTGCAATGA GAACTCGCTG 
TTCAAGAGTT TGTCCCGCTA TCTGGTGCGC CGCAGGGACC CTGAGCTATG GGCCAGTGTG 
CTTCTGGAGA CCAACCCCTT CAGGAGGCCC CTTATTGACC AGGTGGTGCA GACAGCCCTG 
TCAGAGACTC AGGACCCAGA GGAGGTGTCA GTCACAGTGA AGGCCTTCAT GACTGCTGAC 
CTGCCCAATG AACTCATTGA GCTGCTGGAA AAGATTGTCC TGGACAACTC CGTCTTCAGT 
GAACACAGAA ACCTGCAGAA CCTGCTCATC CTAACAGCCA TCAAAGCTGA CCGTACTCGG 
GTCATGGAGT ACATCAACCG CCTGGACAAC TACGATGCCC CAGACATCGC CAACATCGCC 
ATCAGCAGCG AGCTGTTTGA GGAGGCCTTC GCCATCTTCA AGAAATTCGA TGTCAACACC 
TCTGCAGTGC AGGTTCTGAT CGAGCACATT GGTAATCTGG ACCGGGCCTA TGAGTTTGCG 
GAGCGCTGCA ATGAACCTGC GGTGTGGAGT CAACTGGCCA AGGCTCAGCT TCAGAAGGGT 
CTCGTCAAAG AGTCCATCGA CTCCTACATC AAAGCTGATG ATCCTTCAGC TTACATGGAA 
GTGGTCCAAG CTGCTGACCA AAGTGGAAAC TGGGAGGACC TGGTGAAGTT TTTGCAGATG 
GCCAGAAAGA AGGCCAGAGA GTCTTACGTG GAGACTGAGC TGATCTTCGC TCTGGCCAAA 
ACCAACCGTC TCGCTGAACT GGAGGAGTTT ATCAATGGAC CAAATAATGC ACACATCCAG 
CAGGTTGGAG ATCGCTGCTA TGATGAGAAG ATGTACGAGG CGGCCAAACT TCTCTACAAT 
AACGTCTCCA ACTTCGGCCG CTTGGCTTCC ACTCTGGTCC ACTTGGGCGA GTATCAAGCC 
GCTGTCGATG GTGCCCGCAA GGCCAACAGC ACTCGCACAT GGAAAGAGGT TTGCTTTGCC 
TGTGTGGATG GAAAGGAGTT CAGATTGGCT CAAATGTGCG GCCTTCATAT CGTGGTTCAC 
GCAGATGAAT TGGAAGAGCT CATCAACTAC TATCAGGACC GTGGCTACTT TGAGGAGCTG 
ATCACCATGC TGGAAGCGGC GCTGGGGTTG GAGCGCGCTC ATATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA ATTCAAGCCA CAGAAGATGA GGGAGCACCT GGAGCTCTTC 
TGGTCCAGAG TCAACATCCC AAAGGTTTTG AGGGCTGCAG AGCAGGCTCA CCTGTGGGCA 
GAACTGGTTT TCCTGTACGA CAAGTATGAA GAGTTTGATA ACGCCATCAT CACCATGATG 
AGCCATCCCA CAGACGCCTG GAAAGAGGGA CAGTTCAAGG ACATCATCAC CAAGGTGGCC 
AATGTGGAAC TGTACTACAA AGCCATCCAG TTTTATCTGG AATTCAGGCC CTTGTTACTG 
AACGACCTGC TCATAGTTCT TTCTCCAAGA CTCGATCACT CGCGAGCCGT CAACTTCTTC 
AGCAAGGTTA AGCAGCTTCC TCTGGTCAAG CCTTACCTGC GGTCAGTACA AAACCACAAC 
AACAAGTCAG TCAATGAAGC ACTTAACAAC CTCTTCATCT CAGAGGAGGA CTATCAGGCC 
CTCAGGACGT CCATAGACGC GTACGATAAC TTCGACAACA TCTCTCTGGC TCAGCGTCTG 
GAGAAACACG AGTTAATTGA GTTCAGGAGA ATCGCTGCTT ACCTCTTCAA GGGGAACAAC 
CGCTGGAAGC AGAGCGTGGA GCTGTGCAAG AAAGACAAGC TCTACAAGGA TGCCATGCAA 
TATGCATCGG AGTCAAAGGA CACAGAGCTG GCCGAGGAGC TGCTGCAGTG GTTCCTGGAT 
GAGAATAAGA AGGAGTGTTT TGCCGCCTGC CTCTTCACCT GCTACGATCT GCTCCGGCCT 
GACGTGGTGC TGGAGACATC CTGGAGGAAC AACATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAATA CCTCAGCAAG GTGGACAAGC TAGAGACCTC TGAGTCCTTG 
AGGAAAGAGG AGGAGCAAGC GACAGAAACT CAACCAATTG TTTATGGCAC ACCCCAGCTC 
ATGCTGACAG CAGGCCCCAG TGTGCCCGTC CCACCACAGC AGGGGTATGG CTATGGTTAC 
ACAGCAGCTC CTGGTTATCC CCCTCAGGCT CCTCAGGCCC AGCCGGGCTT CGGTTATGGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172697.1
CDS	283..5328
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_005172640.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172640.3

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGAAGC TAAACTCACT GACCAGCTGC CTCTCATTAT TGTGTGCGAT 
CGCTTCGACT TCGTCCACGA CCTTGTTCTT TACCTGTACA GAAACAACCT GCAGAAGTAC 
ATCGAAATCT ACGTCCAGAA GGTAAACCCA AGCCGTCTGC CTGTGGTGAT TGGAGGTCTG 
CTGGATGTGG ACTGCTCTGA GGATGTCATC AAAAACCTGA TCCTGGTTGT ACGGGGACAG 
TTTTCCACCG ATGAGCTGGT GGCAGAAGTG GAGAAGAGAA ACAGACTGAA GCTGTTGTTG 
CCCTGGCTGG AGTCTCGTAT CCACGAGGGC TGTGAGGAGC CAGCTACCCA CAATGCCCTG 
GCCAAGATCT ACATCGACAG CAACAACAAC CCTGAACGCT TCCTGCGGGA GAACCCATAC 
TACGATAGCC GCGTGGTGGG GAAATACTGC GAAAAACGAG ACCCTCATCT GGCCTGTGTG 
GCCTATGAGA GAGGAACGTG TGACCAGGAG CTGATCAACG TTTGCAATGA GAACTCGCTG 
TTCAAGAGTT TGTCCCGCTA TCTGGTGCGC CGCAGGGACC CTGAGCTATG GGCCAGTGTG 
CTTCTGGAGA CCAACCCCTT CAGGAGGCCC CTTATTGACC AGGTGGTGCA GACAGCCCTG 
TCAGAGACTC AGGACCCAGA GGAGGTGTCA GTCACAGTGA AGGCCTTCAT GACTGCTGAC 
CTGCCCAATG AACTCATTGA GCTGCTGGAA AAGATTGTCC TGGACAACTC CGTCTTCAGT 
GAACACAGAA ACCTGCAGAA CCTGCTCATC CTAACAGCCA TCAAAGCTGA CCGTACTCGG 
GTCATGGAGT ACATCAACCG CCTGGACAAC TACGATGCCC CAGACATCGC CAACATCGCC 
ATCAGCAGCG AGCTGTTTGA GGAGGCCTTC GCCATCTTCA AGAAATTCGA TGTCAACACC 
TCTGCAGTGC AGGTTCTGAT CGAGCACATT GGTAATCTGG ACCGGGCCTA TGAGTTTGCG 
GAGCGCTGCA ATGAACCTGC GGTGTGGAGT CAACTGGCCA AGGCTCAGCT TCAGAAGGGT 
CTCGTCAAAG AGTCCATCGA CTCCTACATC AAAGCTGATG ATCCTTCAGC TTACATGGAA 
GTGGTCCAAG CTGCTGACCA AAGTGGAAAC TGGGAGGACC TGGTGAAGTT TTTGCAGATG 
GCCAGAAAGA AGGCCAGAGA GTCTTACGTG GAGACTGAGC TGATCTTCGC TCTGGCCAAA 
ACCAACCGTC TCGCTGAACT GGAGGAGTTT ATCAATGGAC CAAATAATGC ACACATCCAG 
CAGGTTGGAG ATCGCTGCTA TGATGAGAAG ATGTACGAGG CGGCCAAACT TCTCTACAAT 
AACGTCTCCA ACTTCGGCCG CTTGGCTTCC ACTCTGGTCC ACTTGGGCGA GTATCAAGCC 
GCTGTCGATG GTGCCCGCAA GGCCAACAGC ACTCGCACAT GGAAAGAGGT TTGCTTTGCC 
TGTGTGGATG GAAAGGAGTT CAGATTGGCT CAAATGTGCG GCCTTCATAT CGTGGTTCAC 
GCAGATGAAT TGGAAGAGCT CATCAACTAC TATCAGGACC GTGGCTACTT TGAGGAGCTG 
ATCACCATGC TGGAAGCGGC GCTGGGGTTG GAGCGCGCTC ATATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA ATTCAAGCCA CAGAAGATGA GGGAGCACCT GGAGCTCTTC 
TGGTCCAGAG TCAACATCCC AAAGGTTTTG AGGGCTGCAG AGCAGGCTCA CCTGTGGGCA 
GAACTGGTTT TCCTGTACGA CAAGTATGAA GAGTTTGATA ACGCCATCAT CACCATGATG 
AGCCATCCCA CAGACGCCTG GAAAGAGGGA CAGTTCAAGG ACATCATCAC CAAGGTGGCC 
AATGTGGAAC TGTACTACAA AGCCATCCAG TTTTATCTGG AATTCAGGCC CTTGTTACTG 
AACGACCTGC TCATAGTTCT TTCTCCAAGA CTCGATCACT CGCGAGCCGT CAACTTCTTC 
AGCAAGGTTA AGCAGCTTCC TCTGGTCAAG CCTTACCTGC GGTCAGTACA AAACCACAAC 
AACAAGTCAG TCAATGAAGC ACTTAACAAC CTCTTCATCT CAGAGGAGGA CTATCAGGCC 
CTCAGGACGT CCATAGACGC GTACGATAAC TTCGACAACA TCTCTCTGGC TCAGCGTCTG 
GAGAAACACG AGTTAATTGA GTTCAGGAGA ATCGCTGCTT ACCTCTTCAA GGGGAACAAC 
CGCTGGAAGC AGAGCGTGGA GCTGTGCAAG AAAGACAAGC TCTACAAGGA TGCCATGCAA 
TATGCATCGG AGTCAAAGGA CACAGAGCTG GCCGAGGAGC TGCTGCAGTG GTTCCTGGAT 
GAGAATAAGA AGGAGTGTTT TGCCGCCTGC CTCTTCACCT GCTACGATCT GCTCCGGCCT 
GACGTGGTGC TGGAGACATC CTGGAGGAAC AACATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAATA CCTCAGCAAG GTGGACAAGC TAGAGACCTC TGAGTCCTTG 
AGGAAAGAGG AGGAGCAAGC GACAGAAACT CAACCAATTG TTTATGGCAC ACCCCAGCTC 
ATGCTGACAG CAGGCCCCAG TGTGCCCGTC CCACCACAGC AGGGGTATGG CTATGGTTAC 
ACAGCAGCTC CTGGTTATCC CCCTCAGGCT CCTCAGGCCC AGCCGGGCTT CGGTTATGGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_009305357.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303632.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.

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CloneID	ODa39771
Clone ID Related Accession (Same CDS sequence)	XM_009305357.2 , XM_009305357.3
Accession Version	XM_009305357.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	clathrin, heavy polypeptide a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGACTT GAATCAGGAA GCTAAACTCA CTGACCAGCT GCCTCTCATT 
ATTGTGTGCG ATCGCTTCGA CTTCGTCCAC GACCTTGTTC TTTACCTGTA CAGAAACAAC 
CTGCAGAAGT ACATCGAAAT CTACGTCCAG AAGGTAAACC CAAGCCGTCT GCCTGTGGTG 
ATTGGAGGTC TGCTGGATGT GGACTGCTCT GAGGATGTCA TCAAAAACCT GATCCTGGTT 
GTACGGGGAC AGTTTTCCAC CGATGAGCTG GTGGCAGAAG TGGAGAAGAG AAACAGACTG 
AAGCTGTTGT TGCCCTGGCT GGAGTCTCGT ATCCACGAGG GCTGTGAGGA GCCAGCTACC 
CACAATGCCC TGGCCAAGAT CTACATCGAC AGCAACAACA ACCCTGAACG CTTCCTGCGG 
GAGAACCCAT ACTACGATAG CCGCGTGGTG GGGAAATACT GCGAAAAACG AGACCCTCAT 
CTGGCCTGTG TGGCCTATGA GAGAGGAACG TGTGACCAGG AGCTGATCAA CGTTTGCAAT 
GAGAACTCGC TGTTCAAGAG TTTGTCCCGC TATCTGGTGC GCCGCAGGGA CCCTGAGCTA 
TGGGCCAGTG TGCTTCTGGA GACCAACCCC TTCAGGAGGC CCCTTATTGA CCAGGTGGTG 
CAGACAGCCC TGTCAGAGAC TCAGGACCCA GAGGAGGTGT CAGTCACAGT GAAGGCCTTC 
ATGACTGCTG ACCTGCCCAA TGAACTCATT GAGCTGCTGG AAAAGATTGT CCTGGACAAC 
TCCGTCTTCA GTGAACACAG AAACCTGCAG AACCTGCTCA TCCTAACAGC CATCAAAGCT 
GACCGTACTC GGGTCATGGA GTACATCAAC CGCCTGGACA ACTACGATGC CCCAGACATC 
GCCAACATCG CCATCAGCAG CGAGCTGTTT GAGGAGGCCT TCGCCATCTT CAAGAAATTC 
GATGTCAACA CCTCTGCAGT GCAGGTTCTG ATCGAGCACA TTGGTAATCT GGACCGGGCC 
TATGAGTTTG CGGAGCGCTG CAATGAACCT GCGGTGTGGA GTCAACTGGC CAAGGCTCAG 
CTTCAGAAGG GTCTCGTCAA AGAGTCCATC GACTCCTACA TCAAAGCTGA TGATCCTTCA 
GCTTACATGG AAGTGGTCCA AGCTGCTGAC CAAAGTGGAA ACTGGGAGGA CCTGGTGAAG 
TTTTTGCAGA TGGCCAGAAA GAAGGCCAGA GAGTCTTACG TGGAGACTGA GCTGATCTTC 
GCTCTGGCCA AAACCAACCG TCTCGCTGAA CTGGAGGAGT TTATCAATGG ACCAAATAAT 
GCACACATCC AGCAGGTTGG AGATCGCTGC TATGATGAGA AGATGTACGA GGCGGCCAAA 
CTTCTCTACA ATAACGTCTC CAACTTCGGC CGCTTGGCTT CCACTCTGGT CCACTTGGGC 
GAGTATCAAG CCGCTGTCGA TGGTGCCCGC AAGGCCAACA GCACTCGCAC ATGGAAAGAG 
GTTTGCTTTG CCTGTGTGGA TGGAAAGGAG TTCAGATTGG CTCAAATGTG CGGCCTTCAT 
ATCGTGGTTC ACGCAGATGA ATTGGAAGAG CTCATCAACT ACTATCAGGA CCGTGGCTAC 
TTTGAGGAGC TGATCACCAT GCTGGAAGCG GCGCTGGGGT TGGAGCGCGC TCATATGGGC 
ATGTTCACAG AGCTCGCCAT CCTCTACTCC AAATTCAAGC CACAGAAGAT GAGGGAGCAC 
CTGGAGCTCT TCTGGTCCAG AGTCAACATC CCAAAGGTTT TGAGGGCTGC AGAGCAGGCT 
CACCTGTGGG CAGAACTGGT TTTCCTGTAC GACAAGTATG AAGAGTTTGA TAACGCCATC 
ATCACCATGA TGAGCCATCC CACAGACGCC TGGAAAGAGG GACAGTTCAA GGACATCATC 
ACCAAGGTGG CCAATGTGGA ACTGTACTAC AAAGCCATCC AGTTTTATCT GGAATTCAGG 
CCCTTGTTAC TGAACGACCT GCTCATAGTT CTTTCTCCAA GACTCGATCA CTCGCGAGCC 
GTCAACTTCT TCAGCAAGGT TAAGCAGCTT CCTCTGGTCA AGCCTTACCT GCGGTCAGTA 
CAAAACCACA ACAACAAGTC AGTCAATGAA GCACTTAACA ACCTCTTCAT CTCAGAGGAG 
GACTATCAGG CCCTCAGGAC GTCCATAGAC GCGTACGATA ACTTCGACAA CATCTCTCTG 
GCTCAGCGTC TGGAGAAACA CGAGTTAATT GAGTTCAGGA GAATCGCTGC TTACCTCTTC 
AAGGGGAACA ACCGCTGGAA GCAGAGCGTG GAGCTGTGCA AGAAAGACAA GCTCTACAAG 
GATGCCATGC AATATGCATC GGAGTCAAAG GACACAGAGC TGGCCGAGGA GCTGCTGCAG 
TGGTTCCTGG ATGAGAATAA GAAGGAGTGT TTTGCCGCCT GCCTCTTCAC CTGCTACGAT 
CTGCTCCGGC CTGACGTGGT GCTGGAGACA TCCTGGAGGA ACAACATCAT GGACTTCGCC 
ATGCCGTACT TCATCCAGGT CATGAGGGAA TACCTCAGCA AGGTGGACAA GCTAGAGACC 
TCTGAGTCCT TGAGGAAAGA GGAGGAGCAA GCGACAGAAA CTCAACCAAT TGTTTATGGC 
ACACCCCAGC TCATGCTGAC AGCAGGCCCC AGTGTGCCCG TCCCACCACA GCAGGGGTAT 
GGCTATGGTT ACACAGCAGC TCCTGGTTAT CCCCCTCAGG CTCCTCAGGC CCAGCCGGGC 
TTCGGTTATG GCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303632.1
CDS	284..5341
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKDLNQEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_009305357.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305357.2

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGACTT GAATCAGGAA GCTAAACTCA CTGACCAGCT GCCTCTCATT 
ATTGTGTGCG ATCGCTTCGA CTTCGTCCAC GACCTTGTTC TTTACCTGTA CAGAAACAAC 
CTGCAGAAGT ACATCGAAAT CTACGTCCAG AAGGTAAACC CAAGCCGTCT GCCTGTGGTG 
ATTGGAGGTC TGCTGGATGT GGACTGCTCT GAGGATGTCA TCAAAAACCT GATCCTGGTT 
GTACGGGGAC AGTTTTCCAC CGATGAGCTG GTGGCAGAAG TGGAGAAGAG AAACAGACTG 
AAGCTGTTGT TGCCCTGGCT GGAGTCTCGT ATCCACGAGG GCTGTGAGGA GCCAGCTACC 
CACAATGCCC TGGCCAAGAT CTACATCGAC AGCAACAACA ACCCTGAACG CTTCCTGCGG 
GAGAACCCAT ACTACGATAG CCGCGTGGTG GGGAAATACT GCGAAAAACG AGACCCTCAT 
CTGGCCTGTG TGGCCTATGA GAGAGGAACG TGTGACCAGG AGCTGATCAA CGTTTGCAAT 
GAGAACTCGC TGTTCAAGAG TTTGTCCCGC TATCTGGTGC GCCGCAGGGA CCCTGAGCTA 
TGGGCCAGTG TGCTTCTGGA GACCAACCCC TTCAGGAGGC CCCTTATTGA CCAGGTGGTG 
CAGACAGCCC TGTCAGAGAC TCAGGACCCA GAGGAGGTGT CAGTCACAGT GAAGGCCTTC 
ATGACTGCTG ACCTGCCCAA TGAACTCATT GAGCTGCTGG AAAAGATTGT CCTGGACAAC 
TCCGTCTTCA GTGAACACAG AAACCTGCAG AACCTGCTCA TCCTAACAGC CATCAAAGCT 
GACCGTACTC GGGTCATGGA GTACATCAAC CGCCTGGACA ACTACGATGC CCCAGACATC 
GCCAACATCG CCATCAGCAG CGAGCTGTTT GAGGAGGCCT TCGCCATCTT CAAGAAATTC 
GATGTCAACA CCTCTGCAGT GCAGGTTCTG ATCGAGCACA TTGGTAATCT GGACCGGGCC 
TATGAGTTTG CGGAGCGCTG CAATGAACCT GCGGTGTGGA GTCAACTGGC CAAGGCTCAG 
CTTCAGAAGG GTCTCGTCAA AGAGTCCATC GACTCCTACA TCAAAGCTGA TGATCCTTCA 
GCTTACATGG AAGTGGTCCA AGCTGCTGAC CAAAGTGGAA ACTGGGAGGA CCTGGTGAAG 
TTTTTGCAGA TGGCCAGAAA GAAGGCCAGA GAGTCTTACG TGGAGACTGA GCTGATCTTC 
GCTCTGGCCA AAACCAACCG TCTCGCTGAA CTGGAGGAGT TTATCAATGG ACCAAATAAT 
GCACACATCC AGCAGGTTGG AGATCGCTGC TATGATGAGA AGATGTACGA GGCGGCCAAA 
CTTCTCTACA ATAACGTCTC CAACTTCGGC CGCTTGGCTT CCACTCTGGT CCACTTGGGC 
GAGTATCAAG CCGCTGTCGA TGGTGCCCGC AAGGCCAACA GCACTCGCAC ATGGAAAGAG 
GTTTGCTTTG CCTGTGTGGA TGGAAAGGAG TTCAGATTGG CTCAAATGTG CGGCCTTCAT 
ATCGTGGTTC ACGCAGATGA ATTGGAAGAG CTCATCAACT ACTATCAGGA CCGTGGCTAC 
TTTGAGGAGC TGATCACCAT GCTGGAAGCG GCGCTGGGGT TGGAGCGCGC TCATATGGGC 
ATGTTCACAG AGCTCGCCAT CCTCTACTCC AAATTCAAGC CACAGAAGAT GAGGGAGCAC 
CTGGAGCTCT TCTGGTCCAG AGTCAACATC CCAAAGGTTT TGAGGGCTGC AGAGCAGGCT 
CACCTGTGGG CAGAACTGGT TTTCCTGTAC GACAAGTATG AAGAGTTTGA TAACGCCATC 
ATCACCATGA TGAGCCATCC CACAGACGCC TGGAAAGAGG GACAGTTCAA GGACATCATC 
ACCAAGGTGG CCAATGTGGA ACTGTACTAC AAAGCCATCC AGTTTTATCT GGAATTCAGG 
CCCTTGTTAC TGAACGACCT GCTCATAGTT CTTTCTCCAA GACTCGATCA CTCGCGAGCC 
GTCAACTTCT TCAGCAAGGT TAAGCAGCTT CCTCTGGTCA AGCCTTACCT GCGGTCAGTA 
CAAAACCACA ACAACAAGTC AGTCAATGAA GCACTTAACA ACCTCTTCAT CTCAGAGGAG 
GACTATCAGG CCCTCAGGAC GTCCATAGAC GCGTACGATA ACTTCGACAA CATCTCTCTG 
GCTCAGCGTC TGGAGAAACA CGAGTTAATT GAGTTCAGGA GAATCGCTGC TTACCTCTTC 
AAGGGGAACA ACCGCTGGAA GCAGAGCGTG GAGCTGTGCA AGAAAGACAA GCTCTACAAG 
GATGCCATGC AATATGCATC GGAGTCAAAG GACACAGAGC TGGCCGAGGA GCTGCTGCAG 
TGGTTCCTGG ATGAGAATAA GAAGGAGTGT TTTGCCGCCT GCCTCTTCAC CTGCTACGAT 
CTGCTCCGGC CTGACGTGGT GCTGGAGACA TCCTGGAGGA ACAACATCAT GGACTTCGCC 
ATGCCGTACT TCATCCAGGT CATGAGGGAA TACCTCAGCA AGGTGGACAA GCTAGAGACC 
TCTGAGTCCT TGAGGAAAGA GGAGGAGCAA GCGACAGAAA CTCAACCAAT TGTTTATGGC 
ACACCCCAGC TCATGCTGAC AGCAGGCCCC AGTGTGCCCG TCCCACCACA GCAGGGGTAT 
GGCTATGGTT ACACAGCAGC TCCTGGTTAT CCCCCTCAGG CTCCTCAGGC CCAGCCGGGC 
TTCGGTTATG GCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_009305357.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303632.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.

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CloneID	ODa39771
Clone ID Related Accession (Same CDS sequence)	XM_009305357.2 , XM_009305357.3
Accession Version	XM_009305357.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	clathrin, heavy polypeptide a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGACTT GAATCAGGAA GCTAAACTCA CTGACCAGCT GCCTCTCATT 
ATTGTGTGCG ATCGCTTCGA CTTCGTCCAC GACCTTGTTC TTTACCTGTA CAGAAACAAC 
CTGCAGAAGT ACATCGAAAT CTACGTCCAG AAGGTAAACC CAAGCCGTCT GCCTGTGGTG 
ATTGGAGGTC TGCTGGATGT GGACTGCTCT GAGGATGTCA TCAAAAACCT GATCCTGGTT 
GTACGGGGAC AGTTTTCCAC CGATGAGCTG GTGGCAGAAG TGGAGAAGAG AAACAGACTG 
AAGCTGTTGT TGCCCTGGCT GGAGTCTCGT ATCCACGAGG GCTGTGAGGA GCCAGCTACC 
CACAATGCCC TGGCCAAGAT CTACATCGAC AGCAACAACA ACCCTGAACG CTTCCTGCGG 
GAGAACCCAT ACTACGATAG CCGCGTGGTG GGGAAATACT GCGAAAAACG AGACCCTCAT 
CTGGCCTGTG TGGCCTATGA GAGAGGAACG TGTGACCAGG AGCTGATCAA CGTTTGCAAT 
GAGAACTCGC TGTTCAAGAG TTTGTCCCGC TATCTGGTGC GCCGCAGGGA CCCTGAGCTA 
TGGGCCAGTG TGCTTCTGGA GACCAACCCC TTCAGGAGGC CCCTTATTGA CCAGGTGGTG 
CAGACAGCCC TGTCAGAGAC TCAGGACCCA GAGGAGGTGT CAGTCACAGT GAAGGCCTTC 
ATGACTGCTG ACCTGCCCAA TGAACTCATT GAGCTGCTGG AAAAGATTGT CCTGGACAAC 
TCCGTCTTCA GTGAACACAG AAACCTGCAG AACCTGCTCA TCCTAACAGC CATCAAAGCT 
GACCGTACTC GGGTCATGGA GTACATCAAC CGCCTGGACA ACTACGATGC CCCAGACATC 
GCCAACATCG CCATCAGCAG CGAGCTGTTT GAGGAGGCCT TCGCCATCTT CAAGAAATTC 
GATGTCAACA CCTCTGCAGT GCAGGTTCTG ATCGAGCACA TTGGTAATCT GGACCGGGCC 
TATGAGTTTG CGGAGCGCTG CAATGAACCT GCGGTGTGGA GTCAACTGGC CAAGGCTCAG 
CTTCAGAAGG GTCTCGTCAA AGAGTCCATC GACTCCTACA TCAAAGCTGA TGATCCTTCA 
GCTTACATGG AAGTGGTCCA AGCTGCTGAC CAAAGTGGAA ACTGGGAGGA CCTGGTGAAG 
TTTTTGCAGA TGGCCAGAAA GAAGGCCAGA GAGTCTTACG TGGAGACTGA GCTGATCTTC 
GCTCTGGCCA AAACCAACCG TCTCGCTGAA CTGGAGGAGT TTATCAATGG ACCAAATAAT 
GCACACATCC AGCAGGTTGG AGATCGCTGC TATGATGAGA AGATGTACGA GGCGGCCAAA 
CTTCTCTACA ATAACGTCTC CAACTTCGGC CGCTTGGCTT CCACTCTGGT CCACTTGGGC 
GAGTATCAAG CCGCTGTCGA TGGTGCCCGC AAGGCCAACA GCACTCGCAC ATGGAAAGAG 
GTTTGCTTTG CCTGTGTGGA TGGAAAGGAG TTCAGATTGG CTCAAATGTG CGGCCTTCAT 
ATCGTGGTTC ACGCAGATGA ATTGGAAGAG CTCATCAACT ACTATCAGGA CCGTGGCTAC 
TTTGAGGAGC TGATCACCAT GCTGGAAGCG GCGCTGGGGT TGGAGCGCGC TCATATGGGC 
ATGTTCACAG AGCTCGCCAT CCTCTACTCC AAATTCAAGC CACAGAAGAT GAGGGAGCAC 
CTGGAGCTCT TCTGGTCCAG AGTCAACATC CCAAAGGTTT TGAGGGCTGC AGAGCAGGCT 
CACCTGTGGG CAGAACTGGT TTTCCTGTAC GACAAGTATG AAGAGTTTGA TAACGCCATC 
ATCACCATGA TGAGCCATCC CACAGACGCC TGGAAAGAGG GACAGTTCAA GGACATCATC 
ACCAAGGTGG CCAATGTGGA ACTGTACTAC AAAGCCATCC AGTTTTATCT GGAATTCAGG 
CCCTTGTTAC TGAACGACCT GCTCATAGTT CTTTCTCCAA GACTCGATCA CTCGCGAGCC 
GTCAACTTCT TCAGCAAGGT TAAGCAGCTT CCTCTGGTCA AGCCTTACCT GCGGTCAGTA 
CAAAACCACA ACAACAAGTC AGTCAATGAA GCACTTAACA ACCTCTTCAT CTCAGAGGAG 
GACTATCAGG CCCTCAGGAC GTCCATAGAC GCGTACGATA ACTTCGACAA CATCTCTCTG 
GCTCAGCGTC TGGAGAAACA CGAGTTAATT GAGTTCAGGA GAATCGCTGC TTACCTCTTC 
AAGGGGAACA ACCGCTGGAA GCAGAGCGTG GAGCTGTGCA AGAAAGACAA GCTCTACAAG 
GATGCCATGC AATATGCATC GGAGTCAAAG GACACAGAGC TGGCCGAGGA GCTGCTGCAG 
TGGTTCCTGG ATGAGAATAA GAAGGAGTGT TTTGCCGCCT GCCTCTTCAC CTGCTACGAT 
CTGCTCCGGC CTGACGTGGT GCTGGAGACA TCCTGGAGGA ACAACATCAT GGACTTCGCC 
ATGCCGTACT TCATCCAGGT CATGAGGGAA TACCTCAGCA AGGTGGACAA GCTAGAGACC 
TCTGAGTCCT TGAGGAAAGA GGAGGAGCAA GCGACAGAAA CTCAACCAAT TGTTTATGGC 
ACACCCCAGC TCATGCTGAC AGCAGGCCCC AGTGTGCCCG TCCCACCACA GCAGGGGTAT 
GGCTATGGTT ACACAGCAGC TCCTGGTTAT CCCCCTCAGG CTCCTCAGGC CCAGCCGGGC 
TTCGGTTATG GCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303632.1
CDS	268..5325
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKDLNQEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_009305357.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305357.3

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG ACGCTGCCAA AACCCTTCAG ATCTTTAATA TCGAGATGAA GAGTAAGATG 
AAAGCCCACA CCATGACCGA TGACGTTACT TTCTGGAAGT GGATCTCCCT CAACACTGTT 
GCCCTCGTGA CCGATAACGC CGTCTATCAT TGGAGCATGG AGGGAGACTC TCAGCCGGTC 
AAAGTGTTTG ACAGACATTC CAGTCTGGCA GGCTGTCAAA TCATCAACTA CCGCACTGAT 
GCCAAGCAGA AGTGGCTGCT GCTCATTGGC ATTTCTGCTC AGCAAAACAG AGTTGTTGGA 
GCCATGCAGC TCTACTCTGT TGATAGGAAG GTGTCACAGC CTATTGAGGG GCACGCCGCT 
GGCTTTGGTC AATTCAAAAT GGAGGGAAAC ACTGAAGAAT CCACTCTCTT CTGTTTCGCT 
GTGCGAGGTC AGGCAGGCGG AAAGCTTCAC ATTATTGAAG TGGGCACACC ACCAACAGGC 
AACCAGCAAT TCCCAAAGAA AGCCGTGGAT GTGTTCTTCC CACCAGAAGC ACAGAATGAC 
TTCCCTGTAG CCATGCAGAT CAGCTCCAAA CATGATGTTG TGTATCTCAT CACTAAATAT 
GGCTACATTC ATCTGTATGA CCTGGAGACG GGCACCTGCA TCTACATGAA CCGAATCAGC 
GGGGAGACCA TTTTTGTGAC TGCGCCCCAT GATGCCACAG CTGGGATTAT CGGCGTCAAC 
AGGAAAGGAC AGGTGTTGTC AGTGTGTGTT GAGGAGGAAA ACATCATCCA GTACATTACC 
AATGTGCTGC AGAATCCAGA CCTGGCTTTA CGGATGGCAG TCCGCAATAA TCTGGCTGGA 
GCTGAGGAGC TGTTCGGACG CAAGTTTAAC AACCTCTTTG CCGGTGGAAA TTTTGCAGAG 
GCAGCTAAAG TAGCTGCTAA TGCTCCAAAG GGTATCCTGC GCACCCCTGA CACCATACGC 
CGGTTCCAGA GTGTCCCGGC TCAACCAGGC CAGACGTCCC CACTGCTGCA GTATTTTGGG 
ATCCTCCTGG ACCAGGGCCA GCTCAACAAG TTTGAGTCTT TGGAGCTCTG CAGACCCGTC 
CTTCAGCAGG GCCGCAAACA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GCTTGAATGC 
TCAGAGGAGC TGGGTGACCT GGTGAAGTCT GTGGACCCGA CTCTTGCTCT CAGCGTCTAT 
CTGAGGGCCA ACGTACCCAA CAAAGTCATT CAGTGTTTTG CAGAAACTGG CCAGTTCCCT 
AAGATTGTCC TGTACGCCAA AAAGGTGGGC TACACTCCAG ACTGGATCTT CCTGCTGAGG 
AACGTGATGC GGATCAGTCC AGATCAGGGT CTGCAGTTTG CTCAGATGCT TGTTCAGGAC 
GAGGAACCGC TGGCTGACAT CACACAGATC GTTGATGTGT TTATGGAGTA TAACCTGGTC 
CAGCAGTGCA CTTCCTTCCT TTTAGATGCT TTGAAGAACA ACCGGCCATC AGAAGGACCA 
CTGCAGACCC GTCTGCTGGA GATGAACCTG ATGCACGCAC CACAGGTTGC AGATGCCATC 
CTAGGAAACC AGATGTTCAC CAACTACGAT CGTGCCCACA TCGCCCAACT GTGTGAGAAA 
GCAGGACTTC TTCAGAGAGC TTTAGAGCAT TACACCGACT TGTATGACAT CAAACGAGCT 
GTCGTGCACA CACACCTGCT CAACCCGGAG TGGCTGGTCA ACTACTTTGG CTCTCTGTCA 
GTGGAGGACT CTCTGGAGTG TCTGAGGGCC ATGCTGTCTG CTAACATCAG ACAGAATCTC 
CAGATCTGCG TCCAGGTGGC GTCCAAGTAT CACGAGCAGC TGAGCACACA GTCCCTCACC 
GAGCTCTTTG AGTCGTTCAA GAGTTTTGAG GGCCTTTTCT ACTTTCTTGG CTCAATCGTA 
AACTTCAGCC AGGACCCAGA GGTGCATTTC AAATACATCC AGGCCGCATG CAAGACGGGC 
CAAATCAAAG AGGTGGAGCG CATCTGTCGG GAAAGCAACT GCTACAACCC TGAACGTGTC 
AAGAACTTTC TCAAGGACTT GAATCAGGAA GCTAAACTCA CTGACCAGCT GCCTCTCATT 
ATTGTGTGCG ATCGCTTCGA CTTCGTCCAC GACCTTGTTC TTTACCTGTA CAGAAACAAC 
CTGCAGAAGT ACATCGAAAT CTACGTCCAG AAGGTAAACC CAAGCCGTCT GCCTGTGGTG 
ATTGGAGGTC TGCTGGATGT GGACTGCTCT GAGGATGTCA TCAAAAACCT GATCCTGGTT 
GTACGGGGAC AGTTTTCCAC CGATGAGCTG GTGGCAGAAG TGGAGAAGAG AAACAGACTG 
AAGCTGTTGT TGCCCTGGCT GGAGTCTCGT ATCCACGAGG GCTGTGAGGA GCCAGCTACC 
CACAATGCCC TGGCCAAGAT CTACATCGAC AGCAACAACA ACCCTGAACG CTTCCTGCGG 
GAGAACCCAT ACTACGATAG CCGCGTGGTG GGGAAATACT GCGAAAAACG AGACCCTCAT 
CTGGCCTGTG TGGCCTATGA GAGAGGAACG TGTGACCAGG AGCTGATCAA CGTTTGCAAT 
GAGAACTCGC TGTTCAAGAG TTTGTCCCGC TATCTGGTGC GCCGCAGGGA CCCTGAGCTA 
TGGGCCAGTG TGCTTCTGGA GACCAACCCC TTCAGGAGGC CCCTTATTGA CCAGGTGGTG 
CAGACAGCCC TGTCAGAGAC TCAGGACCCA GAGGAGGTGT CAGTCACAGT GAAGGCCTTC 
ATGACTGCTG ACCTGCCCAA TGAACTCATT GAGCTGCTGG AAAAGATTGT CCTGGACAAC 
TCCGTCTTCA GTGAACACAG AAACCTGCAG AACCTGCTCA TCCTAACAGC CATCAAAGCT 
GACCGTACTC GGGTCATGGA GTACATCAAC CGCCTGGACA ACTACGATGC CCCAGACATC 
GCCAACATCG CCATCAGCAG CGAGCTGTTT GAGGAGGCCT TCGCCATCTT CAAGAAATTC 
GATGTCAACA CCTCTGCAGT GCAGGTTCTG ATCGAGCACA TTGGTAATCT GGACCGGGCC 
TATGAGTTTG CGGAGCGCTG CAATGAACCT GCGGTGTGGA GTCAACTGGC CAAGGCTCAG 
CTTCAGAAGG GTCTCGTCAA AGAGTCCATC GACTCCTACA TCAAAGCTGA TGATCCTTCA 
GCTTACATGG AAGTGGTCCA AGCTGCTGAC CAAAGTGGAA ACTGGGAGGA CCTGGTGAAG 
TTTTTGCAGA TGGCCAGAAA GAAGGCCAGA GAGTCTTACG TGGAGACTGA GCTGATCTTC 
GCTCTGGCCA AAACCAACCG TCTCGCTGAA CTGGAGGAGT TTATCAATGG ACCAAATAAT 
GCACACATCC AGCAGGTTGG AGATCGCTGC TATGATGAGA AGATGTACGA GGCGGCCAAA 
CTTCTCTACA ATAACGTCTC CAACTTCGGC CGCTTGGCTT CCACTCTGGT CCACTTGGGC 
GAGTATCAAG CCGCTGTCGA TGGTGCCCGC AAGGCCAACA GCACTCGCAC ATGGAAAGAG 
GTTTGCTTTG CCTGTGTGGA TGGAAAGGAG TTCAGATTGG CTCAAATGTG CGGCCTTCAT 
ATCGTGGTTC ACGCAGATGA ATTGGAAGAG CTCATCAACT ACTATCAGGA CCGTGGCTAC 
TTTGAGGAGC TGATCACCAT GCTGGAAGCG GCGCTGGGGT TGGAGCGCGC TCATATGGGC 
ATGTTCACAG AGCTCGCCAT CCTCTACTCC AAATTCAAGC CACAGAAGAT GAGGGAGCAC 
CTGGAGCTCT TCTGGTCCAG AGTCAACATC CCAAAGGTTT TGAGGGCTGC AGAGCAGGCT 
CACCTGTGGG CAGAACTGGT TTTCCTGTAC GACAAGTATG AAGAGTTTGA TAACGCCATC 
ATCACCATGA TGAGCCATCC CACAGACGCC TGGAAAGAGG GACAGTTCAA GGACATCATC 
ACCAAGGTGG CCAATGTGGA ACTGTACTAC AAAGCCATCC AGTTTTATCT GGAATTCAGG 
CCCTTGTTAC TGAACGACCT GCTCATAGTT CTTTCTCCAA GACTCGATCA CTCGCGAGCC 
GTCAACTTCT TCAGCAAGGT TAAGCAGCTT CCTCTGGTCA AGCCTTACCT GCGGTCAGTA 
CAAAACCACA ACAACAAGTC AGTCAATGAA GCACTTAACA ACCTCTTCAT CTCAGAGGAG 
GACTATCAGG CCCTCAGGAC GTCCATAGAC GCGTACGATA ACTTCGACAA CATCTCTCTG 
GCTCAGCGTC TGGAGAAACA CGAGTTAATT GAGTTCAGGA GAATCGCTGC TTACCTCTTC 
AAGGGGAACA ACCGCTGGAA GCAGAGCGTG GAGCTGTGCA AGAAAGACAA GCTCTACAAG 
GATGCCATGC AATATGCATC GGAGTCAAAG GACACAGAGC TGGCCGAGGA GCTGCTGCAG 
TGGTTCCTGG ATGAGAATAA GAAGGAGTGT TTTGCCGCCT GCCTCTTCAC CTGCTACGAT 
CTGCTCCGGC CTGACGTGGT GCTGGAGACA TCCTGGAGGA ACAACATCAT GGACTTCGCC 
ATGCCGTACT TCATCCAGGT CATGAGGGAA TACCTCAGCA AGGTGGACAA GCTAGAGACC 
TCTGAGTCCT TGAGGAAAGA GGAGGAGCAA GCGACAGAAA CTCAACCAAT TGTTTATGGC 
ACACCCCAGC TCATGCTGAC AGCAGGCCCC AGTGTGCCCG TCCCACCACA GCAGGGGTAT 
GGCTATGGTT ACACAGCAGC TCCTGGTTAT CCCCCTCAGG CTCCTCAGGC CCAGCCGGGC 
TTCGGTTATG GCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_009305358.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303633.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.

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CloneID	ODa39772
Clone ID Related Accession (Same CDS sequence)	XM_009305358.2 , XM_009305358.3
Accession Version	XM_009305358.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	clathrin, heavy polypeptide a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGACTTGAA TCAGGAAGCT AAACTCACTG ACCAGCTGCC TCTCATTATT 
GTGTGCGATC GCTTCGACTT CGTCCACGAC CTTGTTCTTT ACCTGTACAG AAACAACCTG 
CAGAAGTACA TCGAAATCTA CGTCCAGAAG GTAAACCCAA GCCGTCTGCC TGTGGTGATT 
GGAGGTCTGC TGGATGTGGA CTGCTCTGAG GATGTCATCA AAAACCTGAT CCTGGTTGTA 
CGGGGACAGT TTTCCACCGA TGAGCTGGTG GCAGAAGTGG AGAAGAGAAA CAGACTGAAG 
CTGTTGTTGC CCTGGCTGGA GTCTCGTATC CACGAGGGCT GTGAGGAGCC AGCTACCCAC 
AATGCCCTGG CCAAGATCTA CATCGACAGC AACAACAACC CTGAACGCTT CCTGCGGGAG 
AACCCATACT ACGATAGCCG CGTGGTGGGG AAATACTGCG AAAAACGAGA CCCTCATCTG 
GCCTGTGTGG CCTATGAGAG AGGAACGTGT GACCAGGAGC TGATCAACGT TTGCAATGAG 
AACTCGCTGT TCAAGAGTTT GTCCCGCTAT CTGGTGCGCC GCAGGGACCC TGAGCTATGG 
GCCAGTGTGC TTCTGGAGAC CAACCCCTTC AGGAGGCCCC TTATTGACCA GGTGGTGCAG 
ACAGCCCTGT CAGAGACTCA GGACCCAGAG GAGGTGTCAG TCACAGTGAA GGCCTTCATG 
ACTGCTGACC TGCCCAATGA ACTCATTGAG CTGCTGGAAA AGATTGTCCT GGACAACTCC 
GTCTTCAGTG AACACAGAAA CCTGCAGAAC CTGCTCATCC TAACAGCCAT CAAAGCTGAC 
CGTACTCGGG TCATGGAGTA CATCAACCGC CTGGACAACT ACGATGCCCC AGACATCGCC 
AACATCGCCA TCAGCAGCGA GCTGTTTGAG GAGGCCTTCG CCATCTTCAA GAAATTCGAT 
GTCAACACCT CTGCAGTGCA GGTTCTGATC GAGCACATTG GTAATCTGGA CCGGGCCTAT 
GAGTTTGCGG AGCGCTGCAA TGAACCTGCG GTGTGGAGTC AACTGGCCAA GGCTCAGCTT 
CAGAAGGGTC TCGTCAAAGA GTCCATCGAC TCCTACATCA AAGCTGATGA TCCTTCAGCT 
TACATGGAAG TGGTCCAAGC TGCTGACCAA AGTGGAAACT GGGAGGACCT GGTGAAGTTT 
TTGCAGATGG CCAGAAAGAA GGCCAGAGAG TCTTACGTGG AGACTGAGCT GATCTTCGCT 
CTGGCCAAAA CCAACCGTCT CGCTGAACTG GAGGAGTTTA TCAATGGACC AAATAATGCA 
CACATCCAGC AGGTTGGAGA TCGCTGCTAT GATGAGAAGA TGTACGAGGC GGCCAAACTT 
CTCTACAATA ACGTCTCCAA CTTCGGCCGC TTGGCTTCCA CTCTGGTCCA CTTGGGCGAG 
TATCAAGCCG CTGTCGATGG TGCCCGCAAG GCCAACAGCA CTCGCACATG GAAAGAGGTT 
TGCTTTGCCT GTGTGGATGG AAAGGAGTTC AGATTGGCTC AAATGTGCGG CCTTCATATC 
GTGGTTCACG CAGATGAATT GGAAGAGCTC ATCAACTACT ATCAGGACCG TGGCTACTTT 
GAGGAGCTGA TCACCATGCT GGAAGCGGCG CTGGGGTTGG AGCGCGCTCA TATGGGCATG 
TTCACAGAGC TCGCCATCCT CTACTCCAAA TTCAAGCCAC AGAAGATGAG GGAGCACCTG 
GAGCTCTTCT GGTCCAGAGT CAACATCCCA AAGGTTTTGA GGGCTGCAGA GCAGGCTCAC 
CTGTGGGCAG AACTGGTTTT CCTGTACGAC AAGTATGAAG AGTTTGATAA CGCCATCATC 
ACCATGATGA GCCATCCCAC AGACGCCTGG AAAGAGGGAC AGTTCAAGGA CATCATCACC 
AAGGTGGCCA ATGTGGAACT GTACTACAAA GCCATCCAGT TTTATCTGGA ATTCAGGCCC 
TTGTTACTGA ACGACCTGCT CATAGTTCTT TCTCCAAGAC TCGATCACTC GCGAGCCGTC 
AACTTCTTCA GCAAGGTTAA GCAGCTTCCT CTGGTCAAGC CTTACCTGCG GTCAGTACAA 
AACCACAACA ACAAGTCAGT CAATGAAGCA CTTAACAACC TCTTCATCTC AGAGGAGGAC 
TATCAGGCCC TCAGGACGTC CATAGACGCG TACGATAACT TCGACAACAT CTCTCTGGCT 
CAGCGTCTGG AGAAACACGA GTTAATTGAG TTCAGGAGAA TCGCTGCTTA CCTCTTCAAG 
GGGAACAACC GCTGGAAGCA GAGCGTGGAG CTGTGCAAGA AAGACAAGCT CTACAAGGAT 
GCCATGCAAT ATGCATCGGA GTCAAAGGAC ACAGAGCTGG CCGAGGAGCT GCTGCAGTGG 
TTCCTGGATG AGAATAAGAA GGAGTGTTTT GCCGCCTGCC TCTTCACCTG CTACGATCTG 
CTCCGGCCTG ACGTGGTGCT GGAGACATCC TGGAGGAACA ACATCATGGA CTTCGCCATG 
CCGTACTTCA TCCAGGTCAT GAGGGAATAC CTCAGCAAGG TGGACAAGCT AGAGACCTCT 
GAGTCCTTGA GGAAAGAGGA GGAGCAAGCG ACAGAAACTC AACCAATTGT TTATGGCACA 
CCCCAGCTCA TGCTGACAGC AGGCCCCAGT GTGCCCGTCC CACCACAGCA GGGGTATGGC 
TATGGTTACA CAGCAGCTCC TGGTTATCCC CCTCAGGCTC CTCAGGCCCA GCCGGGCTTC 
GGTTATGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303633.1
CDS	284..5338
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKDLNQEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_009305358.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305358.2

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGACTTGAA TCAGGAAGCT AAACTCACTG ACCAGCTGCC TCTCATTATT 
GTGTGCGATC GCTTCGACTT CGTCCACGAC CTTGTTCTTT ACCTGTACAG AAACAACCTG 
CAGAAGTACA TCGAAATCTA CGTCCAGAAG GTAAACCCAA GCCGTCTGCC TGTGGTGATT 
GGAGGTCTGC TGGATGTGGA CTGCTCTGAG GATGTCATCA AAAACCTGAT CCTGGTTGTA 
CGGGGACAGT TTTCCACCGA TGAGCTGGTG GCAGAAGTGG AGAAGAGAAA CAGACTGAAG 
CTGTTGTTGC CCTGGCTGGA GTCTCGTATC CACGAGGGCT GTGAGGAGCC AGCTACCCAC 
AATGCCCTGG CCAAGATCTA CATCGACAGC AACAACAACC CTGAACGCTT CCTGCGGGAG 
AACCCATACT ACGATAGCCG CGTGGTGGGG AAATACTGCG AAAAACGAGA CCCTCATCTG 
GCCTGTGTGG CCTATGAGAG AGGAACGTGT GACCAGGAGC TGATCAACGT TTGCAATGAG 
AACTCGCTGT TCAAGAGTTT GTCCCGCTAT CTGGTGCGCC GCAGGGACCC TGAGCTATGG 
GCCAGTGTGC TTCTGGAGAC CAACCCCTTC AGGAGGCCCC TTATTGACCA GGTGGTGCAG 
ACAGCCCTGT CAGAGACTCA GGACCCAGAG GAGGTGTCAG TCACAGTGAA GGCCTTCATG 
ACTGCTGACC TGCCCAATGA ACTCATTGAG CTGCTGGAAA AGATTGTCCT GGACAACTCC 
GTCTTCAGTG AACACAGAAA CCTGCAGAAC CTGCTCATCC TAACAGCCAT CAAAGCTGAC 
CGTACTCGGG TCATGGAGTA CATCAACCGC CTGGACAACT ACGATGCCCC AGACATCGCC 
AACATCGCCA TCAGCAGCGA GCTGTTTGAG GAGGCCTTCG CCATCTTCAA GAAATTCGAT 
GTCAACACCT CTGCAGTGCA GGTTCTGATC GAGCACATTG GTAATCTGGA CCGGGCCTAT 
GAGTTTGCGG AGCGCTGCAA TGAACCTGCG GTGTGGAGTC AACTGGCCAA GGCTCAGCTT 
CAGAAGGGTC TCGTCAAAGA GTCCATCGAC TCCTACATCA AAGCTGATGA TCCTTCAGCT 
TACATGGAAG TGGTCCAAGC TGCTGACCAA AGTGGAAACT GGGAGGACCT GGTGAAGTTT 
TTGCAGATGG CCAGAAAGAA GGCCAGAGAG TCTTACGTGG AGACTGAGCT GATCTTCGCT 
CTGGCCAAAA CCAACCGTCT CGCTGAACTG GAGGAGTTTA TCAATGGACC AAATAATGCA 
CACATCCAGC AGGTTGGAGA TCGCTGCTAT GATGAGAAGA TGTACGAGGC GGCCAAACTT 
CTCTACAATA ACGTCTCCAA CTTCGGCCGC TTGGCTTCCA CTCTGGTCCA CTTGGGCGAG 
TATCAAGCCG CTGTCGATGG TGCCCGCAAG GCCAACAGCA CTCGCACATG GAAAGAGGTT 
TGCTTTGCCT GTGTGGATGG AAAGGAGTTC AGATTGGCTC AAATGTGCGG CCTTCATATC 
GTGGTTCACG CAGATGAATT GGAAGAGCTC ATCAACTACT ATCAGGACCG TGGCTACTTT 
GAGGAGCTGA TCACCATGCT GGAAGCGGCG CTGGGGTTGG AGCGCGCTCA TATGGGCATG 
TTCACAGAGC TCGCCATCCT CTACTCCAAA TTCAAGCCAC AGAAGATGAG GGAGCACCTG 
GAGCTCTTCT GGTCCAGAGT CAACATCCCA AAGGTTTTGA GGGCTGCAGA GCAGGCTCAC 
CTGTGGGCAG AACTGGTTTT CCTGTACGAC AAGTATGAAG AGTTTGATAA CGCCATCATC 
ACCATGATGA GCCATCCCAC AGACGCCTGG AAAGAGGGAC AGTTCAAGGA CATCATCACC 
AAGGTGGCCA ATGTGGAACT GTACTACAAA GCCATCCAGT TTTATCTGGA ATTCAGGCCC 
TTGTTACTGA ACGACCTGCT CATAGTTCTT TCTCCAAGAC TCGATCACTC GCGAGCCGTC 
AACTTCTTCA GCAAGGTTAA GCAGCTTCCT CTGGTCAAGC CTTACCTGCG GTCAGTACAA 
AACCACAACA ACAAGTCAGT CAATGAAGCA CTTAACAACC TCTTCATCTC AGAGGAGGAC 
TATCAGGCCC TCAGGACGTC CATAGACGCG TACGATAACT TCGACAACAT CTCTCTGGCT 
CAGCGTCTGG AGAAACACGA GTTAATTGAG TTCAGGAGAA TCGCTGCTTA CCTCTTCAAG 
GGGAACAACC GCTGGAAGCA GAGCGTGGAG CTGTGCAAGA AAGACAAGCT CTACAAGGAT 
GCCATGCAAT ATGCATCGGA GTCAAAGGAC ACAGAGCTGG CCGAGGAGCT GCTGCAGTGG 
TTCCTGGATG AGAATAAGAA GGAGTGTTTT GCCGCCTGCC TCTTCACCTG CTACGATCTG 
CTCCGGCCTG ACGTGGTGCT GGAGACATCC TGGAGGAACA ACATCATGGA CTTCGCCATG 
CCGTACTTCA TCCAGGTCAT GAGGGAATAC CTCAGCAAGG TGGACAAGCT AGAGACCTCT 
GAGTCCTTGA GGAAAGAGGA GGAGCAAGCG ACAGAAACTC AACCAATTGT TTATGGCACA 
CCCCAGCTCA TGCTGACAGC AGGCCCCAGT GTGCCCGTCC CACCACAGCA GGGGTATGGC 
TATGGTTACA CAGCAGCTCC TGGTTATCCC CCTCAGGCTC CTCAGGCCCA GCCGGGCTTC 
GGTTATGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cltca ( XM_009305358.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303633.1 Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.

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CloneID	ODa39772
Clone ID Related Accession (Same CDS sequence)	XM_009305358.2 , XM_009305358.3
Accession Version	XM_009305358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	clathrin, heavy polypeptide a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGACTTGAA TCAGGAAGCT AAACTCACTG ACCAGCTGCC TCTCATTATT 
GTGTGCGATC GCTTCGACTT CGTCCACGAC CTTGTTCTTT ACCTGTACAG AAACAACCTG 
CAGAAGTACA TCGAAATCTA CGTCCAGAAG GTAAACCCAA GCCGTCTGCC TGTGGTGATT 
GGAGGTCTGC TGGATGTGGA CTGCTCTGAG GATGTCATCA AAAACCTGAT CCTGGTTGTA 
CGGGGACAGT TTTCCACCGA TGAGCTGGTG GCAGAAGTGG AGAAGAGAAA CAGACTGAAG 
CTGTTGTTGC CCTGGCTGGA GTCTCGTATC CACGAGGGCT GTGAGGAGCC AGCTACCCAC 
AATGCCCTGG CCAAGATCTA CATCGACAGC AACAACAACC CTGAACGCTT CCTGCGGGAG 
AACCCATACT ACGATAGCCG CGTGGTGGGG AAATACTGCG AAAAACGAGA CCCTCATCTG 
GCCTGTGTGG CCTATGAGAG AGGAACGTGT GACCAGGAGC TGATCAACGT TTGCAATGAG 
AACTCGCTGT TCAAGAGTTT GTCCCGCTAT CTGGTGCGCC GCAGGGACCC TGAGCTATGG 
GCCAGTGTGC TTCTGGAGAC CAACCCCTTC AGGAGGCCCC TTATTGACCA GGTGGTGCAG 
ACAGCCCTGT CAGAGACTCA GGACCCAGAG GAGGTGTCAG TCACAGTGAA GGCCTTCATG 
ACTGCTGACC TGCCCAATGA ACTCATTGAG CTGCTGGAAA AGATTGTCCT GGACAACTCC 
GTCTTCAGTG AACACAGAAA CCTGCAGAAC CTGCTCATCC TAACAGCCAT CAAAGCTGAC 
CGTACTCGGG TCATGGAGTA CATCAACCGC CTGGACAACT ACGATGCCCC AGACATCGCC 
AACATCGCCA TCAGCAGCGA GCTGTTTGAG GAGGCCTTCG CCATCTTCAA GAAATTCGAT 
GTCAACACCT CTGCAGTGCA GGTTCTGATC GAGCACATTG GTAATCTGGA CCGGGCCTAT 
GAGTTTGCGG AGCGCTGCAA TGAACCTGCG GTGTGGAGTC AACTGGCCAA GGCTCAGCTT 
CAGAAGGGTC TCGTCAAAGA GTCCATCGAC TCCTACATCA AAGCTGATGA TCCTTCAGCT 
TACATGGAAG TGGTCCAAGC TGCTGACCAA AGTGGAAACT GGGAGGACCT GGTGAAGTTT 
TTGCAGATGG CCAGAAAGAA GGCCAGAGAG TCTTACGTGG AGACTGAGCT GATCTTCGCT 
CTGGCCAAAA CCAACCGTCT CGCTGAACTG GAGGAGTTTA TCAATGGACC AAATAATGCA 
CACATCCAGC AGGTTGGAGA TCGCTGCTAT GATGAGAAGA TGTACGAGGC GGCCAAACTT 
CTCTACAATA ACGTCTCCAA CTTCGGCCGC TTGGCTTCCA CTCTGGTCCA CTTGGGCGAG 
TATCAAGCCG CTGTCGATGG TGCCCGCAAG GCCAACAGCA CTCGCACATG GAAAGAGGTT 
TGCTTTGCCT GTGTGGATGG AAAGGAGTTC AGATTGGCTC AAATGTGCGG CCTTCATATC 
GTGGTTCACG CAGATGAATT GGAAGAGCTC ATCAACTACT ATCAGGACCG TGGCTACTTT 
GAGGAGCTGA TCACCATGCT GGAAGCGGCG CTGGGGTTGG AGCGCGCTCA TATGGGCATG 
TTCACAGAGC TCGCCATCCT CTACTCCAAA TTCAAGCCAC AGAAGATGAG GGAGCACCTG 
GAGCTCTTCT GGTCCAGAGT CAACATCCCA AAGGTTTTGA GGGCTGCAGA GCAGGCTCAC 
CTGTGGGCAG AACTGGTTTT CCTGTACGAC AAGTATGAAG AGTTTGATAA CGCCATCATC 
ACCATGATGA GCCATCCCAC AGACGCCTGG AAAGAGGGAC AGTTCAAGGA CATCATCACC 
AAGGTGGCCA ATGTGGAACT GTACTACAAA GCCATCCAGT TTTATCTGGA ATTCAGGCCC 
TTGTTACTGA ACGACCTGCT CATAGTTCTT TCTCCAAGAC TCGATCACTC GCGAGCCGTC 
AACTTCTTCA GCAAGGTTAA GCAGCTTCCT CTGGTCAAGC CTTACCTGCG GTCAGTACAA 
AACCACAACA ACAAGTCAGT CAATGAAGCA CTTAACAACC TCTTCATCTC AGAGGAGGAC 
TATCAGGCCC TCAGGACGTC CATAGACGCG TACGATAACT TCGACAACAT CTCTCTGGCT 
CAGCGTCTGG AGAAACACGA GTTAATTGAG TTCAGGAGAA TCGCTGCTTA CCTCTTCAAG 
GGGAACAACC GCTGGAAGCA GAGCGTGGAG CTGTGCAAGA AAGACAAGCT CTACAAGGAT 
GCCATGCAAT ATGCATCGGA GTCAAAGGAC ACAGAGCTGG CCGAGGAGCT GCTGCAGTGG 
TTCCTGGATG AGAATAAGAA GGAGTGTTTT GCCGCCTGCC TCTTCACCTG CTACGATCTG 
CTCCGGCCTG ACGTGGTGCT GGAGACATCC TGGAGGAACA ACATCATGGA CTTCGCCATG 
CCGTACTTCA TCCAGGTCAT GAGGGAATAC CTCAGCAAGG TGGACAAGCT AGAGACCTCT 
GAGTCCTTGA GGAAAGAGGA GGAGCAAGCG ACAGAAACTC AACCAATTGT TTATGGCACA 
CCCCAGCTCA TGCTGACAGC AGGCCCCAGT GTGCCCGTCC CACCACAGCA GGGGTATGGC 
TATGGTTACA CAGCAGCTCC TGGTTATCCC CCTCAGGCTC CTCAGGCCCA GCCGGGCTTC 
GGTTATGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303633.1
CDS	268..5322
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICVREKVGEQAQVVIIDMADPNTPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGDSQPVKVFDRHSSLAGCQIINYRTDAKQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAGFGQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQQFPKKAVDVFFPPEAQNDFPVAMQISSKHDVVYLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHDATAGIIGVNRKGQVLSVCVEEENIIQYITNVLQNPDLALRMAVRNNLAGAEELFGRKFNNLFAGGNFAEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQFPKIVLYAKKVGYTPDWIFLLRNVMRISPDQGLQFAQMLVQDEEPLADITQIVDVFMEYNLVQQCTSFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTNYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLTELFESFKSFEGLFYFLGSIVNFSQDPEVHFKYIQAACKTGQIKEVERICRESNCYNPERVKNFLKDLNQEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGTCDQELINVCNENSLFKSLSRYLVRRRDPELWASVLLETNPFRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISSELFEEAFAIFKKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGLVKESIDSYIKADDPSAYMEVVQAADQSGNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYEAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEFDNAIITMMSHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFRPLLLNDLLIVLSPRLDHSRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNEALNNLFISEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDKLYKDAMQYASESKDTELAEELLQWFLDENKKECFAACLFTCYDLLRPDVVLETSWRNNIMDFAMPYFIQVMREYLSKVDKLETSESLRKEEEQATETQPIVYGTPQLMLTAGPSVPVPPQQGYGYGYTAAPGYPPQAPQAQPGFGYGM

Target ORF information:

RefSeq Version	XM_009305358.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio clathrin, heavy chain a (Hc) (cltca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305358.3

ORF Insert Sequence:

ATGGCTCAAA TCCTGCCCAT CAGATTCCAG GAGCACCTGC AGCTCCAAAA TTTGGGGATC 
AACCCAGCCA ACATTGGGTT CAGCACCCTG ACAATGGAAT CGGATAAGTT CATCTGTGTC 
CGGGAGAAGG TTGGAGAACA GGCGCAGGTG GTGATCATTG ATATGGCGGA CCCAAACACC 
CCCATCCGCA GGCCCATCTC TGCAGATAGT GCCATCATGA ACCCTGCGAG CAAAGTCATT 
GCCCTCAAAG CTGCCAAAAC CCTTCAGATC TTTAATATCG AGATGAAGAG TAAGATGAAA 
GCCCACACCA TGACCGATGA CGTTACTTTC TGGAAGTGGA TCTCCCTCAA CACTGTTGCC 
CTCGTGACCG ATAACGCCGT CTATCATTGG AGCATGGAGG GAGACTCTCA GCCGGTCAAA 
GTGTTTGACA GACATTCCAG TCTGGCAGGC TGTCAAATCA TCAACTACCG CACTGATGCC 
AAGCAGAAGT GGCTGCTGCT CATTGGCATT TCTGCTCAGC AAAACAGAGT TGTTGGAGCC 
ATGCAGCTCT ACTCTGTTGA TAGGAAGGTG TCACAGCCTA TTGAGGGGCA CGCCGCTGGC 
TTTGGTCAAT TCAAAATGGA GGGAAACACT GAAGAATCCA CTCTCTTCTG TTTCGCTGTG 
CGAGGTCAGG CAGGCGGAAA GCTTCACATT ATTGAAGTGG GCACACCACC AACAGGCAAC 
CAGCAATTCC CAAAGAAAGC CGTGGATGTG TTCTTCCCAC CAGAAGCACA GAATGACTTC 
CCTGTAGCCA TGCAGATCAG CTCCAAACAT GATGTTGTGT ATCTCATCAC TAAATATGGC 
TACATTCATC TGTATGACCT GGAGACGGGC ACCTGCATCT ACATGAACCG AATCAGCGGG 
GAGACCATTT TTGTGACTGC GCCCCATGAT GCCACAGCTG GGATTATCGG CGTCAACAGG 
AAAGGACAGG TGTTGTCAGT GTGTGTTGAG GAGGAAAACA TCATCCAGTA CATTACCAAT 
GTGCTGCAGA ATCCAGACCT GGCTTTACGG ATGGCAGTCC GCAATAATCT GGCTGGAGCT 
GAGGAGCTGT TCGGACGCAA GTTTAACAAC CTCTTTGCCG GTGGAAATTT TGCAGAGGCA 
GCTAAAGTAG CTGCTAATGC TCCAAAGGGT ATCCTGCGCA CCCCTGACAC CATACGCCGG 
TTCCAGAGTG TCCCGGCTCA ACCAGGCCAG ACGTCCCCAC TGCTGCAGTA TTTTGGGATC 
CTCCTGGACC AGGGCCAGCT CAACAAGTTT GAGTCTTTGG AGCTCTGCAG ACCCGTCCTT 
CAGCAGGGCC GCAAACAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGCT TGAATGCTCA 
GAGGAGCTGG GTGACCTGGT GAAGTCTGTG GACCCGACTC TTGCTCTCAG CGTCTATCTG 
AGGGCCAACG TACCCAACAA AGTCATTCAG TGTTTTGCAG AAACTGGCCA GTTCCCTAAG 
ATTGTCCTGT ACGCCAAAAA GGTGGGCTAC ACTCCAGACT GGATCTTCCT GCTGAGGAAC 
GTGATGCGGA TCAGTCCAGA TCAGGGTCTG CAGTTTGCTC AGATGCTTGT TCAGGACGAG 
GAACCGCTGG CTGACATCAC ACAGATCGTT GATGTGTTTA TGGAGTATAA CCTGGTCCAG 
CAGTGCACTT CCTTCCTTTT AGATGCTTTG AAGAACAACC GGCCATCAGA AGGACCACTG 
CAGACCCGTC TGCTGGAGAT GAACCTGATG CACGCACCAC AGGTTGCAGA TGCCATCCTA 
GGAAACCAGA TGTTCACCAA CTACGATCGT GCCCACATCG CCCAACTGTG TGAGAAAGCA 
GGACTTCTTC AGAGAGCTTT AGAGCATTAC ACCGACTTGT ATGACATCAA ACGAGCTGTC 
GTGCACACAC ACCTGCTCAA CCCGGAGTGG CTGGTCAACT ACTTTGGCTC TCTGTCAGTG 
GAGGACTCTC TGGAGTGTCT GAGGGCCATG CTGTCTGCTA ACATCAGACA GAATCTCCAG 
ATCTGCGTCC AGGTGGCGTC CAAGTATCAC GAGCAGCTGA GCACACAGTC CCTCACCGAG 
CTCTTTGAGT CGTTCAAGAG TTTTGAGGGC CTTTTCTACT TTCTTGGCTC AATCGTAAAC 
TTCAGCCAGG ACCCAGAGGT GCATTTCAAA TACATCCAGG CCGCATGCAA GACGGGCCAA 
ATCAAAGAGG TGGAGCGCAT CTGTCGGGAA AGCAACTGCT ACAACCCTGA ACGTGTCAAG 
AACTTTCTCA AGGACTTGAA TCAGGAAGCT AAACTCACTG ACCAGCTGCC TCTCATTATT 
GTGTGCGATC GCTTCGACTT CGTCCACGAC CTTGTTCTTT ACCTGTACAG AAACAACCTG 
CAGAAGTACA TCGAAATCTA CGTCCAGAAG GTAAACCCAA GCCGTCTGCC TGTGGTGATT 
GGAGGTCTGC TGGATGTGGA CTGCTCTGAG GATGTCATCA AAAACCTGAT CCTGGTTGTA 
CGGGGACAGT TTTCCACCGA TGAGCTGGTG GCAGAAGTGG AGAAGAGAAA CAGACTGAAG 
CTGTTGTTGC CCTGGCTGGA GTCTCGTATC CACGAGGGCT GTGAGGAGCC AGCTACCCAC 
AATGCCCTGG CCAAGATCTA CATCGACAGC AACAACAACC CTGAACGCTT CCTGCGGGAG 
AACCCATACT ACGATAGCCG CGTGGTGGGG AAATACTGCG AAAAACGAGA CCCTCATCTG 
GCCTGTGTGG CCTATGAGAG AGGAACGTGT GACCAGGAGC TGATCAACGT TTGCAATGAG 
AACTCGCTGT TCAAGAGTTT GTCCCGCTAT CTGGTGCGCC GCAGGGACCC TGAGCTATGG 
GCCAGTGTGC TTCTGGAGAC CAACCCCTTC AGGAGGCCCC TTATTGACCA GGTGGTGCAG 
ACAGCCCTGT CAGAGACTCA GGACCCAGAG GAGGTGTCAG TCACAGTGAA GGCCTTCATG 
ACTGCTGACC TGCCCAATGA ACTCATTGAG CTGCTGGAAA AGATTGTCCT GGACAACTCC 
GTCTTCAGTG AACACAGAAA CCTGCAGAAC CTGCTCATCC TAACAGCCAT CAAAGCTGAC 
CGTACTCGGG TCATGGAGTA CATCAACCGC CTGGACAACT ACGATGCCCC AGACATCGCC 
AACATCGCCA TCAGCAGCGA GCTGTTTGAG GAGGCCTTCG CCATCTTCAA GAAATTCGAT 
GTCAACACCT CTGCAGTGCA GGTTCTGATC GAGCACATTG GTAATCTGGA CCGGGCCTAT 
GAGTTTGCGG AGCGCTGCAA TGAACCTGCG GTGTGGAGTC AACTGGCCAA GGCTCAGCTT 
CAGAAGGGTC TCGTCAAAGA GTCCATCGAC TCCTACATCA AAGCTGATGA TCCTTCAGCT 
TACATGGAAG TGGTCCAAGC TGCTGACCAA AGTGGAAACT GGGAGGACCT GGTGAAGTTT 
TTGCAGATGG CCAGAAAGAA GGCCAGAGAG TCTTACGTGG AGACTGAGCT GATCTTCGCT 
CTGGCCAAAA CCAACCGTCT CGCTGAACTG GAGGAGTTTA TCAATGGACC AAATAATGCA 
CACATCCAGC AGGTTGGAGA TCGCTGCTAT GATGAGAAGA TGTACGAGGC GGCCAAACTT 
CTCTACAATA ACGTCTCCAA CTTCGGCCGC TTGGCTTCCA CTCTGGTCCA CTTGGGCGAG 
TATCAAGCCG CTGTCGATGG TGCCCGCAAG GCCAACAGCA CTCGCACATG GAAAGAGGTT 
TGCTTTGCCT GTGTGGATGG AAAGGAGTTC AGATTGGCTC AAATGTGCGG CCTTCATATC 
GTGGTTCACG CAGATGAATT GGAAGAGCTC ATCAACTACT ATCAGGACCG TGGCTACTTT 
GAGGAGCTGA TCACCATGCT GGAAGCGGCG CTGGGGTTGG AGCGCGCTCA TATGGGCATG 
TTCACAGAGC TCGCCATCCT CTACTCCAAA TTCAAGCCAC AGAAGATGAG GGAGCACCTG 
GAGCTCTTCT GGTCCAGAGT CAACATCCCA AAGGTTTTGA GGGCTGCAGA GCAGGCTCAC 
CTGTGGGCAG AACTGGTTTT CCTGTACGAC AAGTATGAAG AGTTTGATAA CGCCATCATC 
ACCATGATGA GCCATCCCAC AGACGCCTGG AAAGAGGGAC AGTTCAAGGA CATCATCACC 
AAGGTGGCCA ATGTGGAACT GTACTACAAA GCCATCCAGT TTTATCTGGA ATTCAGGCCC 
TTGTTACTGA ACGACCTGCT CATAGTTCTT TCTCCAAGAC TCGATCACTC GCGAGCCGTC 
AACTTCTTCA GCAAGGTTAA GCAGCTTCCT CTGGTCAAGC CTTACCTGCG GTCAGTACAA 
AACCACAACA ACAAGTCAGT CAATGAAGCA CTTAACAACC TCTTCATCTC AGAGGAGGAC 
TATCAGGCCC TCAGGACGTC CATAGACGCG TACGATAACT TCGACAACAT CTCTCTGGCT 
CAGCGTCTGG AGAAACACGA GTTAATTGAG TTCAGGAGAA TCGCTGCTTA CCTCTTCAAG 
GGGAACAACC GCTGGAAGCA GAGCGTGGAG CTGTGCAAGA AAGACAAGCT CTACAAGGAT 
GCCATGCAAT ATGCATCGGA GTCAAAGGAC ACAGAGCTGG CCGAGGAGCT GCTGCAGTGG 
TTCCTGGATG AGAATAAGAA GGAGTGTTTT GCCGCCTGCC TCTTCACCTG CTACGATCTG 
CTCCGGCCTG ACGTGGTGCT GGAGACATCC TGGAGGAACA ACATCATGGA CTTCGCCATG 
CCGTACTTCA TCCAGGTCAT GAGGGAATAC CTCAGCAAGG TGGACAAGCT AGAGACCTCT 
GAGTCCTTGA GGAAAGAGGA GGAGCAAGCG ACAGAAACTC AACCAATTGT TTATGGCACA 
CCCCAGCTCA TGCTGACAGC AGGCCCCAGT GTGCCCGTCC CACCACAGCA GGGGTATGGC 
TATGGTTACA CAGCAGCTCC TGGTTATCCC CCTCAGGCTC CTCAGGCCCA GCCGGGCTTC 
GGTTATGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis.
The Journal of cell biology200(5)667-79(2013 Mar)
Ellis K,Bagwell J,Bagnat M

Context-dependent proangiogenic function of bone morphogenetic protein signaling is mediated by disabled homolog 2.
Developmental cell23(2)441-8(2012 Aug)
Kim JD,Kang H,Larriv?e B,Lee MY,Mettlen M,Schmid SL,Roman BL,Qyang Y,Eichmann A,Jin SW

A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Genes & development25(21)2306-20(2011 Nov)
Trinh le A,Hochgreb T,Graham M,Wu D,Ruf-Zamojski F,Jayasena CS,Saxena A,Hawk R,Gonzalez-Serricchio A,Dixson A,Chow E,Gonzales C,Leung HY,Solomon I,Bronner-Fraser M,Megason SG,Fraser SE

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Genes & development23(5)619-32(2009 Mar)
Mishima Y,Abreu-Goodger C,Staton AA,Stahlhut C,Shou C,Cheng C,Gerstein M,Enright AJ,Giraldez AJ

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cltca cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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