chpt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chpt1
Entrez Gene ID	322605
Full Name	choline phosphotransferase 1
Synonyms	fd50f07,sycp3,wu:fb67a04,wu:fd50f07
General protein information	Preferred Names choline phosphotransferase 1 Names cholinephosphotransferase 1 diacylglycerol cholinephosphotransferase 1 synaptonemal complex protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212624.1	NP_997789.1	cholinephosphotransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHPT1	XP_003313944.1
Rattus norvegicus (Norway rat)	Chpt1	NP_001007751.1
Xenopus tropicalis (tropical clawed frog)	chpt1	NP_001135694.1
Homo sapiens (human)	CHPT1	NP_064629.2
Bos taurus (cattle)	CHPT1	NP_001068975.1
Mus musculus (house mouse)	Chpt1	NP_001140162.1
Danio rerio (zebrafish)	chpt1	NP_997789.1
Canis lupus familiaris (dog)	CHPT1	XP_005629349.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish.
Ozaki Y, Saito K, Shinya M, Kawasaki T, Sakai N
Gene expression patterns : GEP11(5-6)309-15(2011 Jun-Jul)

Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3.
Hart DO, Raha T, Lawson ND, Green MR
Nature450(7172)1082-5(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunohistochemical observations revealed that Sycp3 is specifically localized in spermatocytes in typical nuclear patterns at each meiotic stage.
Title: Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish.

The following chpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37896	NM_212624.1 Latest version!	Danio rerio choline phosphotransferase 1 (chpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chpt1 ( NM_212624.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997789.1 Danio rerio choline phosphotransferase 1 (chpt1), mRNA.

Price & Availability↑

CloneID	ODa37896
Clone ID Related Accession (Same CDS sequence)	NM_212624.1
Accession Version	NM_212624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	cholinephosphotransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045345.1. ##Evidence-Data-START## Transcript exon combination :: BC045345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476778 [ECO:0000348] ##Evidence-Data-END##

ATGCCCCAGT GCGAGTGTCC GGAGCCCCTG TCCGCTGTCC AGCTCAAGCG CCTGGAGGAG 
CACAAGTACA GCGCGGCCGG GCGATCTCTC TTCGAGCCGC CCTGTCAGAT ATACTGGAAC 
TGGCTGGTCC AGCAGATTCC GACGTGGGTC GCGCCGAACA CGCTGACCAC CATCGGACTG 
GTTATTAACG TAATAACGAC GGTCATTCTC GTGTATTACA GCCCCACGGC TACAGAGGAG 
GTTCCAGGTT GGGCTTTCTT TCTCAGCGCC TTAGGCCTCT TCATCTACCA ATCACTGGAT 
GCCATTGATG GAAAACAGGC CAGACGGACC AATAGCAGCT CAGCTCTAGG AGAGCTCTTT 
GACCATGGCT GTGATGCAGT TTCTACAGTG TTTGTTGCAG TGGGAACGTG TATATGCTGT 
GGGATCGGCG CATATCCTAA CTGGATGTTT TTTTGTGGCT TTGTGGGCAT GTTCATGTTC 
TTCTGCGCAC ACTGGCAGAC ATACGTGTCT GGAACGCTCC GCTTCGGACT GGTTGACGTG 
ACAGAGGTCC AGATTGCTAT TATAATCATG TATTTGTTGA CAGCATTTAC AGGAGTGAGC 
TTTTGGGAAA TGAGGGTGCC AGTTCTTGGT GTGAATTTGC AAACCTTTCC TATTCTTGGT 
ATCATCGGCG GATTCCTCTA TTCAACCTAT AACTACTTCT TTGTTATTAT GAACGGTGGC 
GTCGGCAAAA ACGGCTCAAC TGTTGCAGAC ACCAGTGTTT TGACTCCTGG TCTCCACATC 
GGACTCATTC TCACACTGGC TTTCATCATC TTTAAAAAGT CGTCCAGTCA CCTTTTCGAG 
CACCATCCTT GCCTATATGT TCTCACGTTT GGCATGGTGA TCGCCAAGAT CTCAAATAAG 
CTAGTGGTGG CCCACATGAC CAAGAGTGAG CTTCATCTTC AAGACACAGC TTTCATCGGT 
CCTGGACTGC TCTTCCTTAA CCAGTATTTC AACAGCTACA TTGATGAACA TATCGTGCTT 
TGGATCGCTA TGGTGCTGTC GCTGGTGGAT CTGGTGCGTT ACTGCACAGC CGTATGTCTG 
CAAATCGCCT CTCATCTACG GATCCGGGTT TTCAGCATCT CCCCACAGGG TCATGCGCAC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997789.1
CDS	155..1303
Translation	MPQCECPEPLSAVQLKRLEEHKYSAAGRSLFEPPCQIYWNWLVQQIPTWVAPNTLTTIGLVINVITTVILVYYSPTATEEVPGWAFFLSALGLFIYQSLDAIDGKQARRTNSSSALGELFDHGCDAVSTVFVAVGTCICCGIGAYPNWMFFCGFVGMFMFFCAHWQTYVSGTLRFGLVDVTEVQIAIIIMYLLTAFTGVSFWEMRVPVLGVNLQTFPILGIIGGFLYSTYNYFFVIMNGGVGKNGSTVADTSVLTPGLHIGLILTLAFIIFKKSSSHLFEHHPCLYVLTFGMVIAKISNKLVVAHMTKSELHLQDTAFIGPGLLFLNQYFNSYIDEHIVLWIAMVLSLVDLVRYCTAVCLQIASHLRIRVFSISPQGHAHKD

Target ORF information:

RefSeq Version	NM_212624.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline phosphotransferase 1 (chpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212624.1

ORF Insert Sequence:

ATGCCCCAGT GCGAGTGTCC GGAGCCCCTG TCCGCTGTCC AGCTCAAGCG CCTGGAGGAG 
CACAAGTACA GCGCGGCCGG GCGATCTCTC TTCGAGCCGC CCTGTCAGAT ATACTGGAAC 
TGGCTGGTCC AGCAGATTCC GACGTGGGTC GCGCCGAACA CGCTGACCAC CATCGGACTG 
GTTATTAACG TAATAACGAC GGTCATTCTC GTGTATTACA GCCCCACGGC TACAGAGGAG 
GTTCCAGGTT GGGCTTTCTT TCTCAGCGCC TTAGGCCTCT TCATCTACCA ATCACTGGAT 
GCCATTGATG GAAAACAGGC CAGACGGACC AATAGCAGCT CAGCTCTAGG AGAGCTCTTT 
GACCATGGCT GTGATGCAGT TTCTACAGTG TTTGTTGCAG TGGGAACGTG TATATGCTGT 
GGGATCGGCG CATATCCTAA CTGGATGTTT TTTTGTGGCT TTGTGGGCAT GTTCATGTTC 
TTCTGCGCAC ACTGGCAGAC ATACGTGTCT GGAACGCTCC GCTTCGGACT GGTTGACGTG 
ACAGAGGTCC AGATTGCTAT TATAATCATG TATTTGTTGA CAGCATTTAC AGGAGTGAGC 
TTTTGGGAAA TGAGGGTGCC AGTTCTTGGT GTGAATTTGC AAACCTTTCC TATTCTTGGT 
ATCATCGGCG GATTCCTCTA TTCAACCTAT AACTACTTCT TTGTTATTAT GAACGGTGGC 
GTCGGCAAAA ACGGCTCAAC TGTTGCAGAC ACCAGTGTTT TGACTCCTGG TCTCCACATC 
GGACTCATTC TCACACTGGC TTTCATCATC TTTAAAAAGT CGTCCAGTCA CCTTTTCGAG 
CACCATCCTT GCCTATATGT TCTCACGTTT GGCATGGTGA TCGCCAAGAT CTCAAATAAG 
CTAGTGGTGG CCCACATGAC CAAGAGTGAG CTTCATCTTC AAGACACAGC TTTCATCGGT 
CCTGGACTGC TCTTCCTTAA CCAGTATTTC AACAGCTACA TTGATGAACA TATCGTGCTT 
TGGATCGCTA TGGTGCTGTC GCTGGTGGAT CTGGTGCGTT ACTGCACAGC CGTATGTCTG 
CAAATCGCCT CTCATCTACG GATCCGGGTT TTCAGCATCT CCCCACAGGG TCATGCGCAC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish.
Gene expression patterns : GEP11(5-6)309-15(2011 Jun-Jul)
Ozaki Y,Saito K,Shinya M,Kawasaki T,Sakai N

Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3.
Nature450(7172)1082-5(2007 Dec)
Hart DO,Raha T,Lawson ND,Green MR