chia.6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chia.6
Entrez Gene ID	322420
Full Name	chitinase, acidic.6
Synonyms	wu:fb60f06,zgc:65788
General protein information	Preferred Names chitinase, acidic.6 Names acidic chitinase-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199603.2	NP_955897.2	acidic chitinase-like precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:65788	NP_955897.2
Danio rerio (zebrafish)	chia.4	NP_956740.1
Magnaporthe oryzae (rice blast fungus)	MGG_01247	XP_003714154.1
Bos taurus (cattle)	CHIA	NP_777124.1
Gallus gallus (chicken)	CHIA	NP_989760.1
Danio rerio (zebrafish)	chia.3	NP_998378.1
Homo sapiens (human)	CHIA	NP_970615.2
Xenopus tropicalis (tropical clawed frog)	chia	NP_001186489.1
	CHIA	XP_001104487.1
Canis lupus familiaris (dog)	CHIA	XP_537030.3
Mus musculus (house mouse)	Chia1	NP_075675.2
Rattus norvegicus (Norway rat)	Chia	NP_997469.1
Danio rerio (zebrafish)	zgc:173927	NP_001103511.1
Neurospora crassa	NCU03209	XP_965309.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Choksi SP, Babu D, Lau D, Yu X, Roy S
Development (Cambridge, England)141(17)3410-9(2014 Sep)

Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos.
Koch BE, Stougaard J, Spaink HP
Gene expression patterns : GEP14(2)69-77(2014 Mar)

Deficiency in hematopoietic phosphatase ptpn6/Shp1 hyperactivates the innate immune system and impairs control of bacterial infections in zebrafish embryos.
Kanwal Z, Zakrzewska A, den Hertog J, Spaink HP, Schaaf MJ, Meijer AH
Journal of immunology (Baltimore, Md. : 1950)190(4)1631-45(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chia.6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chia.6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30867	NM_199603.2 Latest version!	Danio rerio chitinase, acidic.6 (chia.6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chia.6 ( NM_199603.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955897.2 Danio rerio chitinase, acidic.6 (chia.6), mRNA.

Price & Availability↑

CloneID	ODa30867
Clone ID Related Accession (Same CDS sequence)	NM_199603.2
Accession Version	NM_199603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	acidic chitinase-like precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR352232.20, EH449168.1 and CN509615.1. On Jul 14, 2011 this sequence version replaced NM_199603.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC046004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGAT TCATTTTTAT AGCCGGCTTT TCACTGGCAT TGTGTCACTT TGGATTGGCG 
TCCCAACTTG TATGTTACTT CACCAACTGG TCTCAGTACA GACCTGATGT TGGCAAGTAC 
ATGCCATCTA ATGTGGATCC ACATCTGTGT ACTCATTTAA TTTATGCCTT TTCCATTATT 
AATAATGAAA ATAAACTGAC AACCTATGAA TGGAATGATG AAACTCTCTA CCAGTCCTTT 
AATGGCCTAA AACAAAGCAA CCCCAACCTG AAAACTCTGT TGGCTGTCGG TGGATGGAAC 
TTTGGCACAA CTCAGTTTAG CAGCATGGTG TCCACGCCAC AAAACAGACA GACCTTTATT 
CAGTCCAGTA TCACATTTCT GAGGACTCAT GGATTTGACG GTCTGGATTT GGACTGGGAG 
TATCCTGGTT CACGTGGAAG TCCTCCAGAA GACAAGCAGA GATTCACTTT GCTCTGTAAG 
GAGCTTGTAG AAGCTTATCA GGCAGAGAGT GCAGCTACTG GACGCCCCAG ACTCATGCTC 
ACTGCTGCAG TGGCTGCTGG GAAAGGAAAC ATTGACGCTG GTTATGAAAT TGCAGAAATT 
GCCAAGTATT TGGATTTCAT CAATATCATG ACCTATGATT TCCACGGCTC ATGGGAAAGT 
GTCACTGGAC ACAACAGTCC TCTGTATCGA GGCTCTGGAG ACATAGGAGA CAAAATCTAC 
TACAATACTG ACTTTGCAAT GACCTACTGG CGAGATCAGG GCGCTCCTGT GGAGAAGCTG 
AGGATGGGCT TTGCTGCTTA TGGAAGAACG TTTCGCCTGT CATCTGCAGT CAGTGGAGTT 
GGAGCTCCAG CTAGTGGTGC TGCTTCAGCT GGAACATACA CCAGAGAAGC TGGATTCTGG 
TCCTATTATG AGATCTGTAC TTTTCTAAAA CAAGCCACTG TGCAACAGAT AGTTGATCAG 
AAAGTTCCTT ATGCAACAAA AGGACAGGAG TGGGTGGGAT TTGATAACAT GGAAAGTTAT 
AAGACTAAGG TGGACTATTT GAAGGAGAAA GGCTTTGGAG GAGCTTTTGT CTGGGCTCTG 
GATCTTGATG ACTTCAGTGG TCAGTTCTGT GGGCAGAGCA AATACCCTCT TATTGGACAA 
CTTCGCACAC TGCTTAATAT CAACAACACT GAGTTCCCAT ATAACCCTCC AAGCACAACA 
GTTAAGCCCA GTGGGAGGCC CACAAGAGCA TCTGCTACTG TACCTTATGC CACAAGACCT 
CCTGTTGTAC CGACGGCGCC CTCAGGATCA GACTTCTGTA CAGGCAAATT AGAGGGCATC 
TACGAATACC CAGCTGACAG CATGATCTAC ATTCATTGTG CCAATGGCAG GACCTTCATA 
CAGAGATGTC CTGCAAAGAC AGTCTTTGAT CCCGACTGCA AATGCTGTAA CTGGCCTAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955897.2
CDS	77..1519
Translation	MSRFIFIAGFSLALCHFGLASQLVCYFTNWSQYRPDVGKYMPSNVDPHLCTHLIYAFSIINNENKLTTYEWNDETLYQSFNGLKQSNPNLKTLLAVGGWNFGTTQFSSMVSTPQNRQTFIQSSITFLRTHGFDGLDLDWEYPGSRGSPPEDKQRFTLLCKELVEAYQAESAATGRPRLMLTAAVAAGKGNIDAGYEIAEIAKYLDFINIMTYDFHGSWESVTGHNSPLYRGSGDIGDKIYYNTDFAMTYWRDQGAPVEKLRMGFAAYGRTFRLSSAVSGVGAPASGAASAGTYTREAGFWSYYEICTFLKQATVQQIVDQKVPYATKGQEWVGFDNMESYKTKVDYLKEKGFGGAFVWALDLDDFSGQFCGQSKYPLIGQLRTLLNINNTEFPYNPPSTTVKPSGRPTRASATVPYATRPPVVPTAPSGSDFCTGKLEGIYEYPADSMIYIHCANGRTFIQRCPAKTVFDPDCKCCNWPK

Target ORF information:

RefSeq Version	NM_199603.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chitinase, acidic.6 (chia.6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199603.2

ORF Insert Sequence:

ATGAGCAGAT TCATTTTTAT AGCCGGCTTT TCACTGGCAT TGTGTCACTT TGGATTGGCG 
TCCCAACTTG TATGTTACTT CACCAACTGG TCTCAGTACA GACCTGATGT TGGCAAGTAC 
ATGCCATCTA ATGTGGATCC ACATCTGTGT ACTCATTTAA TTTATGCCTT TTCCATTATT 
AATAATGAAA ATAAACTGAC AACCTATGAA TGGAATGATG AAACTCTCTA CCAGTCCTTT 
AATGGCCTAA AACAAAGCAA CCCCAACCTG AAAACTCTGT TGGCTGTCGG TGGATGGAAC 
TTTGGCACAA CTCAGTTTAG CAGCATGGTG TCCACGCCAC AAAACAGACA GACCTTTATT 
CAGTCCAGTA TCACATTTCT GAGGACTCAT GGATTTGACG GTCTGGATTT GGACTGGGAG 
TATCCTGGTT CACGTGGAAG TCCTCCAGAA GACAAGCAGA GATTCACTTT GCTCTGTAAG 
GAGCTTGTAG AAGCTTATCA GGCAGAGAGT GCAGCTACTG GACGCCCCAG ACTCATGCTC 
ACTGCTGCAG TGGCTGCTGG GAAAGGAAAC ATTGACGCTG GTTATGAAAT TGCAGAAATT 
GCCAAGTATT TGGATTTCAT CAATATCATG ACCTATGATT TCCACGGCTC ATGGGAAAGT 
GTCACTGGAC ACAACAGTCC TCTGTATCGA GGCTCTGGAG ACATAGGAGA CAAAATCTAC 
TACAATACTG ACTTTGCAAT GACCTACTGG CGAGATCAGG GCGCTCCTGT GGAGAAGCTG 
AGGATGGGCT TTGCTGCTTA TGGAAGAACG TTTCGCCTGT CATCTGCAGT CAGTGGAGTT 
GGAGCTCCAG CTAGTGGTGC TGCTTCAGCT GGAACATACA CCAGAGAAGC TGGATTCTGG 
TCCTATTATG AGATCTGTAC TTTTCTAAAA CAAGCCACTG TGCAACAGAT AGTTGATCAG 
AAAGTTCCTT ATGCAACAAA AGGACAGGAG TGGGTGGGAT TTGATAACAT GGAAAGTTAT 
AAGACTAAGG TGGACTATTT GAAGGAGAAA GGCTTTGGAG GAGCTTTTGT CTGGGCTCTG 
GATCTTGATG ACTTCAGTGG TCAGTTCTGT GGGCAGAGCA AATACCCTCT TATTGGACAA 
CTTCGCACAC TGCTTAATAT CAACAACACT GAGTTCCCAT ATAACCCTCC AAGCACAACA 
GTTAAGCCCA GTGGGAGGCC CACAAGAGCA TCTGCTACTG TACCTTATGC CACAAGACCT 
CCTGTTGTAC CGACGGCGCC CTCAGGATCA GACTTCTGTA CAGGCAAATT AGAGGGCATC 
TACGAATACC CAGCTGACAG CATGATCTAC ATTCATTGTG CCAATGGCAG GACCTTCATA 
CAGAGATGTC CTGCAAAGAC AGTCTTTGAT CCCGACTGCA AATGCTGTAA CTGGCCTAAA 
TAA