lef1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lef1
Entrez Gene ID	30701
Full Name	lymphoid enhancer-binding factor 1
General protein information	Preferred Names lymphoid enhancer-binding factor 1 Names lymphoid enhancer-binding factor 1 etID310027.1 lymphocyte enhancer binding factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131426.1	NP_571501.1	lymphoid enhancer-binding factor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LEF1	XP_001136065.1
Bos taurus (cattle)	LEF1	NP_001179785.1
Rattus norvegicus (Norway rat)	Lef1	NP_569113.1
Canis lupus familiaris (dog)	LEF1	XP_863334.2
Mus musculus (house mouse)	Lef1	NP_034833.2
Gallus gallus (chicken)	LEF1	NP_990344.1
Xenopus tropicalis (tropical clawed frog)	lef1	NP_001230763.1
Homo sapiens (human)	LEF1	NP_057353.1
Danio rerio (zebrafish)	lef1	NP_571501.1

Gene Ontology

The following lef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03375	NM_131426.1 Latest version!	Danio rerio lymphoid enhancer-binding factor 1 (lef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lef1 ( NM_131426.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571501.1 Danio rerio lymphoid enhancer-binding factor 1 (lef1), mRNA.

Price & Availability↑

CloneID	ODa03375
Clone ID Related Accession (Same CDS sequence)	NM_131426.1
Accession Version	NM_131426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	lymphoid enhancer-binding factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF136454.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF136454.1, BC162097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCAGT TGTCAGGTGG AGGAGGCGGC GGGGACCCGG AGCTCTGCGC CACCGATGAA 
ATGATCCCGT TCAAAGACGA GGGAGACCCG CACAAGGAGC AAATCTTCGC GGAGATCAGC 
CACTCCGAGG AGGAGGGAGA CTTAGCGGAG ATCAAATCAT CTCTGGTCAA CGAGACAGAA 
ATCAGTCCCA ACAGTAACAG TCACGACGCA GCTAGACAGT CCCAAATAAC ACCAGACTCT 
TACCATGAGA AGCACAGGGA CCATCCGGAT GATGGCAAAC TCCAAGACTT GTACAGCAAA 
GGCCACCCGT ACCCGAGTTA TCCCGGATAC ATCATGATGA CCAATATGAA CAATGAACCC 
TACATGAACA ACGGCTCTCT CTCGCCTCCC ATTCCCAGAA CGTCGAATAA GGTGCCGGTG 
GTCCAGCCGT CCCATGCCGT TCACCCACTC ACCCCTCTGA TCACATACAG TGACGAGCAC 
TTCGCCCCCG GCCCACATTC GGGACACCAC CCACAAGATG TCAACCCCAA GCAAGCAGGT 
ATGCCCAGGC ATCATCCTGG ACCAGACATT CCCAATTTCT ATCCCCTTTC CCCGGGGGGA 
GTTGGACAGA TGACCCCTCC TCTGGGTTGG TTCTCACATC ACATGGTACC AGGCCCTCCT 
GGACCCCACG CCACAGGAAT CCCCCATCCA GCCATTGTCA ACCCACAGGT GAAACAGGAG 
CACGACACAG ACCTGATGCA CATGAAACCT CAGCACGAGC AGAGAAAGGA GCAGGAGCCC 
AAAAGACCTC ACATCAAGAA ACCTCTAAAC GCTTTCATGC TGTATATGAA AGAGATGCGC 
GCCAATGTGG TGGCCGAATG CACGCTGAAG GAGAGCGCCG CTATCAATCA GATCCTCGGC 
CGGAGGTGGC ATGCTTTATC TCGGGAAGAG CAAGCTAAGT ATTACGAATT AGCCCGCAAG 
GAACGGCAGC TCCATATGCA GCTTTACCCA GGATGGTCTG CCAGAGACAA TTATGGAAAG 
AAAAAAAAGC GGAAGAGGGA AAAGATCCAG GAACCTGCTT CAGGTACAGG CCAGAGAATG 
AAAACGGCGT ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571501.1
CDS	408..1505
Translation	MPQLSGGGGGGDPELCATDEMIPFKDEGDPHKEQIFAEISHSEEEGDLAEIKSSLVNETEISPNSNSHDAARQSQITPDSYHEKHRDHPDDGKLQDLYSKGHPYPSYPGYIMMTNMNNEPYMNNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFAPGPHSGHHPQDVNPKQAGMPRHHPGPDIPNFYPLSPGGVGQMTPPLGWFSHHMVPGPPGPHATGIPHPAIVNPQVKQEHDTDLMHMKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKIQEPASGTGQRMKTAYI

Target ORF information:

RefSeq Version	NM_131426.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lymphoid enhancer-binding factor 1 (lef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131426.1

ORF Insert Sequence:

ATGCCGCAGT TGTCAGGTGG AGGAGGCGGC GGGGACCCGG AGCTCTGCGC CACCGATGAA 
ATGATCCCGT TCAAAGACGA GGGAGACCCG CACAAGGAGC AAATCTTCGC GGAGATCAGC 
CACTCCGAGG AGGAGGGAGA CTTAGCGGAG ATCAAATCAT CTCTGGTCAA CGAGACAGAA 
ATCAGTCCCA ACAGTAACAG TCACGACGCA GCTAGACAGT CCCAAATAAC ACCAGACTCT 
TACCATGAGA AGCACAGGGA CCATCCGGAT GATGGCAAAC TCCAAGACTT GTACAGCAAA 
GGCCACCCGT ACCCGAGTTA TCCCGGATAC ATCATGATGA CCAATATGAA CAATGAACCC 
TACATGAACA ACGGCTCTCT CTCGCCTCCC ATTCCCAGAA CGTCGAATAA GGTGCCGGTG 
GTCCAGCCGT CCCATGCCGT TCACCCACTC ACCCCTCTGA TCACATACAG TGACGAGCAC 
TTCGCCCCCG GCCCACATTC GGGACACCAC CCACAAGATG TCAACCCCAA GCAAGCAGGT 
ATGCCCAGGC ATCATCCTGG ACCAGACATT CCCAATTTCT ATCCCCTTTC CCCGGGGGGA 
GTTGGACAGA TGACCCCTCC TCTGGGTTGG TTCTCACATC ACATGGTACC AGGCCCTCCT 
GGACCCCACG CCACAGGAAT CCCCCATCCA GCCATTGTCA ACCCACAGGT GAAACAGGAG 
CACGACACAG ACCTGATGCA CATGAAACCT CAGCACGAGC AGAGAAAGGA GCAGGAGCCC 
AAAAGACCTC ACATCAAGAA ACCTCTAAAC GCTTTCATGC TGTATATGAA AGAGATGCGC 
GCCAATGTGG TGGCCGAATG CACGCTGAAG GAGAGCGCCG CTATCAATCA GATCCTCGGC 
CGGAGGTGGC ATGCTTTATC TCGGGAAGAG CAAGCTAAGT ATTACGAATT AGCCCGCAAG 
GAACGGCAGC TCCATATGCA GCTTTACCCA GGATGGTCTG CCAGAGACAA TTATGGAAAG 
AAAAAAAAGC GGAAGAGGGA AAAGATCCAG GAACCTGCTT CAGGTACAGG CCAGAGAATG 
AAAACGGCGT ACATCTGA