ccne1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccne1
Entrez Gene ID	30188
Full Name	cyclin E1
Synonyms	ccne,cyce,fa18e07,wu:fa18e07
General protein information	Preferred Names cyclin E1 Names G1/S-specific cyclin-E1 cyclin E
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130995.1	NP_571070.1	G1/S-specific cyclin-E1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCNE1	NP_001229.1
Danio rerio (zebrafish)	ccne1	NP_571070.1
Canis lupus familiaris (dog)	CCNE1	XP_005616959.1
Rattus norvegicus (Norway rat)	Ccne1	NP_001094291.1
Xenopus tropicalis (tropical clawed frog)	ccne1	NP_001016328.1
Pan troglodytes (chimpanzee)	CCNE1	XP_512559.4
Mus musculus (house mouse)	Ccne1	NP_031659.2
	CCNE1	XP_001084995.1
Bos taurus (cattle)	CCNE1	NP_001179705.1
Gallus gallus (chicken)	CCNE1	NP_001026529.1
Drosophila melanogaster (fruit fly)	CycE	NP_476960.1

Gene Ontology

The following ccne1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccne1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01914	NM_130995.1 Latest version!	Danio rerio cyclin E1 (ccne1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccne1 ( NM_130995.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571070.1 Danio rerio cyclin E1 (ccne1), mRNA.

Price & Availability↑

CloneID	ODa01914
Clone ID Related Accession (Same CDS sequence)	NM_130995.1
Accession Version	NM_130995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Danio rerio(zebrafish)
Product	G1/S-specific cyclin-E1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X83594.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X83594.1, BC045842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCAAGCA AGAAAGTGCT ACAAACAGAA CATATCAACA CTACAGATGA AGCTCCTAAA 
ACAACTTCAG TGCGTCCCAG GAAGAGAAAA GCAGACGTGG CTATTCATTT ACAAGATCCA 
GATGAAGAGG TAACAGAGAT GACAAGAAAG AAACAATGTG CATCCCAGGC CTGCTGGAAT 
CCCGACACAG GTTACACAAG CCCATGCAGG CGGATCCCCA CACCTGATGA AGTCGAGGAA 
CCGGTTGCTT TTGGCAGCGT GGGATTTACG CAGTATGCAT CAGAAAGCAT TTTCATTACC 
CCCACACGCT CTACCCCTCT TCCGGCTCTT TGCTGGGCAA GCAAAGATGA AGTTTGGAAC 
AACCTGCTCG GAAAAGACAA GCTTTACCTG CGAGATACAC GCGTTATGGA GAGACATCCA 
AATCTTCAAC CCAAAATGAG AGCGATTCTG CTGGACTGGC TAATGGAGGT TTGTGAGGTG 
TACAAGTTAC ACAGAGAAAC ATTTTATTTG GGTCAGGACT ACTTTGATCG CTTCATGGCC 
ACACAAGAAA ATGTCCTGAA AACAACGCTA CAGCTTATAG GCATCTCCTG TCTCTTTATA 
GCTGCCAAAA TGGAAGAAAT TTACCCTCCT AAGGTGCATC AGTTTGCTTA TGTTACTGAT 
GGGGCCTGCA CAGAGGATGA CATTCTTAGC ATGGAGATTA TCATCATGAA GGAGTTAAAC 
TGGAGTTTGA GTCCTTTAAC CCCGGTTGCT TGGCTCAACA TCTATATGCA GATGGCCTAT 
CTAAAGGAGA CTGCTGAGGT TCTCACGGCC CAGTACCCAC AGGCTACATT TGTGCAGATT 
GCAGAGCTCT TGGATCTGTG TATTCTGGAT GTAAGGAGTC TGGAGTTTTC TTACAGCCTA 
CTTGCAGCTT CAGCACTCTT CCACTTTTCT TCTCTGGAGC TAGTGATAAA AGTTTCAGGG 
CTGAAGTGGT GTGATTTGGA GGAGTGTGTA CGATGGATGG TTCCTTTCGC CATGTCTATT 
CGTGAAGCAG GCAGCTCAGC CCTTAAGACA TTCAAAGGAA TAGCAGCAGA TGATATGCAC 
AATATCCAGA CACATGTGCC TTACTTGGAA TGGCTGGGAA AGGTTCACTC CTATCAGCTA 
GTGGACATTG AGAGCAGTCA GAGGTCTCCG GTTCCCACTG GAGTGCTTAC GCCACCACCA 
AGCAGCGAGA AGCCAGAGAG CACCATCTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571070.1
CDS	192..1424
Translation	MPSKKVLQTEHINTTDEAPKTTSVRPRKRKADVAIHLQDPDEEVTEMTRKKQCASQACWNPDTGYTSPCRRIPTPDEVEEPVAFGSVGFTQYASESIFITPTRSTPLPALCWASKDEVWNNLLGKDKLYLRDTRVMERHPNLQPKMRAILLDWLMEVCEVYKLHRETFYLGQDYFDRFMATQENVLKTTLQLIGISCLFIAAKMEEIYPPKVHQFAYVTDGACTEDDILSMEIIIMKELNWSLSPLTPVAWLNIYMQMAYLKETAEVLTAQYPQATFVQIAELLDLCILDVRSLEFSYSLLAASALFHFSSLELVIKVSGLKWCDLEECVRWMVPFAMSIREAGSSALKTFKGIAADDMHNIQTHVPYLEWLGKVHSYQLVDIESSQRSPVPTGVLTPPPSSEKPESTIS

Target ORF information:

RefSeq Version	NM_130995.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin E1 (ccne1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130995.1

ORF Insert Sequence:

ATGCCAAGCA AGAAAGTGCT ACAAACAGAA CATATCAACA CTACAGATGA AGCTCCTAAA 
ACAACTTCAG TGCGTCCCAG GAAGAGAAAA GCAGACGTGG CTATTCATTT ACAAGATCCA 
GATGAAGAGG TAACAGAGAT GACAAGAAAG AAACAATGTG CATCCCAGGC CTGCTGGAAT 
CCCGACACAG GTTACACAAG CCCATGCAGG CGGATCCCCA CACCTGATGA AGTCGAGGAA 
CCGGTTGCTT TTGGCAGCGT GGGATTTACG CAGTATGCAT CAGAAAGCAT TTTCATTACC 
CCCACACGCT CTACCCCTCT TCCGGCTCTT TGCTGGGCAA GCAAAGATGA AGTTTGGAAC 
AACCTGCTCG GAAAAGACAA GCTTTACCTG CGAGATACAC GCGTTATGGA GAGACATCCA 
AATCTTCAAC CCAAAATGAG AGCGATTCTG CTGGACTGGC TAATGGAGGT TTGTGAGGTG 
TACAAGTTAC ACAGAGAAAC ATTTTATTTG GGTCAGGACT ACTTTGATCG CTTCATGGCC 
ACACAAGAAA ATGTCCTGAA AACAACGCTA CAGCTTATAG GCATCTCCTG TCTCTTTATA 
GCTGCCAAAA TGGAAGAAAT TTACCCTCCT AAGGTGCATC AGTTTGCTTA TGTTACTGAT 
GGGGCCTGCA CAGAGGATGA CATTCTTAGC ATGGAGATTA TCATCATGAA GGAGTTAAAC 
TGGAGTTTGA GTCCTTTAAC CCCGGTTGCT TGGCTCAACA TCTATATGCA GATGGCCTAT 
CTAAAGGAGA CTGCTGAGGT TCTCACGGCC CAGTACCCAC AGGCTACATT TGTGCAGATT 
GCAGAGCTCT TGGATCTGTG TATTCTGGAT GTAAGGAGTC TGGAGTTTTC TTACAGCCTA 
CTTGCAGCTT CAGCACTCTT CCACTTTTCT TCTCTGGAGC TAGTGATAAA AGTTTCAGGG 
CTGAAGTGGT GTGATTTGGA GGAGTGTGTA CGATGGATGG TTCCTTTCGC CATGTCTATT 
CGTGAAGCAG GCAGCTCAGC CCTTAAGACA TTCAAAGGAA TAGCAGCAGA TGATATGCAC 
AATATCCAGA CACATGTGCC TTACTTGGAA TGGCTGGGAA AGGTTCACTC CTATCAGCTA 
GTGGACATTG AGAGCAGTCA GAGGTCTCCG GTTCCCACTG GAGTGCTTAC GCCACCACCA 
AGCAGCGAGA AGCCAGAGAG CACCATCTCT TGA