chrd cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chrd
Entrez Gene ID	30161
Full Name	chordin
Synonyms	chd,din,fe47d04,wu:fe47d04
General protein information	Preferred Names chordin Names chordin chordino dino (din) protein chordino
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130973.3	NP_571048.2	chordin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRD	XP_005639840.1
Danio rerio (zebrafish)	chd	NP_571048.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000472	XP_310620.4
Bos taurus (cattle)	CHRD	XP_002684938.2
Drosophila melanogaster (fruit fly)	sog	NP_001259578.1
Homo sapiens (human)	CHRD	NP_003732.2
Mus musculus (house mouse)	Chrd	NP_001264970.1
Gallus gallus (chicken)	CHRD	NP_990311.1
Pan troglodytes (chimpanzee)	CHRD	XP_516917.3
	CHRD	XP_001097729.2
Rattus norvegicus (Norway rat)	Chrd	NP_476475.1
Xenopus tropicalis (tropical clawed frog)	chrd	NP_001136129.1

Gene Ontology

The following chrd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chrd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98536	NM_130973.3 Latest version!	Danio rerio chordin (chrd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chrd ( NM_130973.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571048.2 Danio rerio chordin (chrd), mRNA.

Price & Availability↑

CloneID	ODa98536
Clone ID Related Accession (Same CDS sequence)	NM_130973.3
Accession Version	NM_130973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	chordin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LO017831.1 and CR381579.22. On Oct 9, 2016 this sequence version replaced NM_130973.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01003871.1, GDQH01028928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAGG GGCTGCTGTG GATTCTGCTG TCCGTTATTA TCGCATCTGT GCACGGATCG 
AGACTCAAGA CACCCGCGCT GCCCATCCAG CCCGAGAGAG AACCCATGAT TTCCAAAGGA 
TTATCCGGTT GCTCCTTCGG TGGCCGCTTT TACTCTCTGG AAGACACGTG GCATCCAGAT 
CTCGGAGAGC CGTTTGGTGT GATGCACTGC GTTATGTGTC ATTGTGAGCC GCAGAGGAGC 
CGGCGAGGGA AGGTTTTCGG GAAAGTAAGC TGCAGGAATA TGAAACAGGA CTGTCCAGAT 
CCGACCTGCG ACGATCCGGT CCTGCTTCCC GGACACTGCT GCAAAACATG CCCAAAAGGC 
GACTCTGGGA GGAAGGAGGT CGAGTCTCTG TTTGATTTCT TCCAGGAAAA AGATGACGAT 
TTGCACAAAT CCTACAACGA CAGATCGTAC ATCAGCTCCG AGGACACCAG CACTCGAGAC 
AGCACCACCA CCGATTTTGT GGCGCTGCTG ACGGGTGTGA CAGACTCTTG GCTGCCGAGC 
TCCAGTGGTG TCGCGAGGGC CAGATTTACA CTTTCTCGAA CAAGTCTGAC TTTCTCCATC 
ACTTTCCAGA GAATAAACAG ACCGAGTCTC ATTGCATTTC TGGACACTGA CGGAAACACC 
GCGTTTGAGT TCAGAGTCCC GCAGGCCGAT AACGACATGA TCTGCGGCAT CTGGAAAAAT 
GTGCCGAAAC CTCACATGCG GCAACTGGAG GCGGAGCAAC TGCACGTCTC CATGACCACC 
GCTGACAACA GGAAGGAGGA GCTTCAGGGC AGAATAATCA AACACAGAGC ACTGTTTGCT 
GAAACGTTCA GTGCAATCCT GACATCTGAC GAGGTGCATT CTGGGATGGG TGGAATCGCC 
ATGTTGACGC TCAGTGACAC CGAAAACAAT CTGCATTTTA TCCTGATTAT GCAGGGACTC 
GTTCCTCCAG GGAGCTCCAA GGTGCCGGTG CGAGTGAAAC TGCAGTATCG ACAACACCTG 
CTGAGAGAAA TACGAGCAAA CATCACTGCA GACGACTCAG ACTTTGCTGA AGTGCTGGCG 
GATCTGAACA GTCGTGAGCT CTTCTGGTTG TCCCGCGGGC AGCTCCAGAT CTCCGTCCAG 
ACTGAAGGTC AAACCCCGCG TCACATCTCG GGATTCATTT CTGGAAGGAG ATCATGTGAC 
ACTCTGCAGA GTGTGTTGTC GAGCGGCGCG GCTCTGACTG CGGGTCAGAC GGGCGGTGTG 
GGCTCTGCTG TCTTCACTCT GCACCCGAAC GGCTCTCTGG ATTACCAGCT GCTGGTGGCG 
GGTCTGAGCA GCGCGGTGCT GAGCGTTAGC ATCGAGATGA AGCCGCGTCG GCGCAACAAA 
CGCAGCGTTC TGTACGAGCT CTCGGCAGTG TTCACAGACC AGCGGGCGGC AGGCAGCTGC 
GGACGCGTGG AGGCCAGACA CACACACATG CTGCTGCAGA ACGAGCTGTT CATCAACATC 
GCCACCGCAC TGCAGCCCGA CGGGGAGCTG CGCGGACAGA TACGCCTGCT GCCATACAAT 
GGACTGGACG CGCGCAGAAA CGAGTTGCCG GTTCCTCTGG CCGGTGTTCT GGTGTCTCCG 
CCGGTGCGCA CTGGTGCAGC GGGTCACGCC TGGGTCTCGG TGGACCCTCA GTGTCATCTG 
CATTACGAGA TCATCGTTAA CGGACTCAGC AAGAGCGAAG ACGCCTCCAT CAGTGCTCAC 
CTGCATGGAT TGGCTGAGAT CGGAGAGATG GACGACTCTT CCACCAATCA CAAGAGACTC 
CTGACTGGTT TCTACGGGCA ACAGGCTCAA GGCGTCCTGA AGGACATCAG TGTTGAATTA 
TTGCGTCATT TAAATGAAGG TACGGCGTAT CTGCAGGTCA GCACCAAGAT GAACCCCAGA 
GGAGAGATTC GCGGACGGAT CCACGTCCCG AACCACTGTG AGTCCCCGGC GCCCCGCGCT 
GAGTTTCTGG AGGAGCCGGA GTTTGAGGAT CTGCTGTTCA CACGGGAGCC GACGGAGCTG 
CGCAAAGACA CACACACTCA CGTACACTCC TGCTTCTTTG AGGGAGAACA ACACACACAC 
GGCTCACAGT GGACACCGCA GTACAACACC TGCTTCACCT GCACCTGCCA GAAAAAGACG 
GTGATCTGTG ACCCAGTGAT GTGTCCTACA CTCTCCTGCA CACACACCGT CCAGCCTGAA 
GACCAATGCT GTCCCATCTG TGAAGAGAAG AAAGAATCTA AAGAGACAGC CGCTGTAGAA 
AAAGTTGAGG AAAATCCTGA AGGCTGTTAT TTCGAAGGCG ATCAGAAAAT GCACGCACCT 
GGAACAACAT GGCATCCGTT CGTCCCGCCG TTCGGCTACA TCAAGTGTGC AGTCTGTACC 
TGCAAGGGCT CGACAGGGGA AGTGCACTGC GAGAAGGTGA CGTGCCCGCC CCTCACCTGC 
AGCCGACCAA TCAGACGAAA CCCTTCAGAC TGCTGTAAGG AGTGTCCTCC TGAAGAAACG 
CCCCCTCTGG AGGACGAGGA GATGATGCAG GCGGACGGGA CGCGGCTCTG CAAATTCGGG 
AAAAACTACT ATCAGAACAG CGAACACTGG CACCCGAGCG TCCCGCTGGT GGGGGAGATG 
AAGTGCATCA CCTGCTGGTG TGACCATGGC GTCACAAAGT GTCAGAGGAA GCAGTGTCCA 
CTGCTGAGCT GCAGAAATCC CATCCGCACA GAGGGAAAAT GCTGTCCGGA ATGCATAGAG 
GACTTCATGG AGAAAGAAGA AATGGCAAAG ATGGCAGAGA AAAAGAAAAG CTGGAGACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571048.2
CDS	150..2972
Translation	MMEGLLWILLSVIIASVHGSRLKTPALPIQPEREPMISKGLSGCSFGGRFYSLEDTWHPDLGEPFGVMHCVMCHCEPQRSRRGKVFGKVSCRNMKQDCPDPTCDDPVLLPGHCCKTCPKGDSGRKEVESLFDFFQEKDDDLHKSYNDRSYISSEDTSTRDSTTTDFVALLTGVTDSWLPSSSGVARARFTLSRTSLTFSITFQRINRPSLIAFLDTDGNTAFEFRVPQADNDMICGIWKNVPKPHMRQLEAEQLHVSMTTADNRKEELQGRIIKHRALFAETFSAILTSDEVHSGMGGIAMLTLSDTENNLHFILIMQGLVPPGSSKVPVRVKLQYRQHLLREIRANITADDSDFAEVLADLNSRELFWLSRGQLQISVQTEGQTPRHISGFISGRRSCDTLQSVLSSGAALTAGQTGGVGSAVFTLHPNGSLDYQLLVAGLSSAVLSVSIEMKPRRRNKRSVLYELSAVFTDQRAAGSCGRVEARHTHMLLQNELFINIATALQPDGELRGQIRLLPYNGLDARRNELPVPLAGVLVSPPVRTGAAGHAWVSVDPQCHLHYEIIVNGLSKSEDASISAHLHGLAEIGEMDDSSTNHKRLLTGFYGQQAQGVLKDISVELLRHLNEGTAYLQVSTKMNPRGEIRGRIHVPNHCESPAPRAEFLEEPEFEDLLFTREPTELRKDTHTHVHSCFFEGEQHTHGSQWTPQYNTCFTCTCQKKTVICDPVMCPTLSCTHTVQPEDQCCPICEEKKESKETAAVEKVEENPEGCYFEGDQKMHAPGTTWHPFVPPFGYIKCAVCTCKGSTGEVHCEKVTCPPLTCSRPIRRNPSDCCKECPPEETPPLEDEEMMQADGTRLCKFGKNYYQNSEHWHPSVPLVGEMKCITCWCDHGVTKCQRKQCPLLSCRNPIRTEGKCCPECIEDFMEKEEMAKMAEKKKSWRH

Target ORF information:

RefSeq Version	NM_130973.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chordin (chrd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130973.3

ORF Insert Sequence:

ATGATGGAGG GGCTGCTGTG GATTCTGCTG TCCGTTATTA TCGCATCTGT GCACGGATCG 
AGACTCAAGA CACCCGCGCT GCCCATCCAG CCCGAGAGAG AACCCATGAT TTCCAAAGGA 
TTATCCGGTT GCTCCTTCGG TGGCCGCTTT TACTCTCTGG AAGACACGTG GCATCCAGAT 
CTCGGAGAGC CGTTTGGTGT GATGCACTGC GTTATGTGTC ATTGTGAGCC GCAGAGGAGC 
CGGCGAGGGA AGGTTTTCGG GAAAGTAAGC TGCAGGAATA TGAAACAGGA CTGTCCAGAT 
CCGACCTGCG ACGATCCGGT CCTGCTTCCC GGACACTGCT GCAAAACATG CCCAAAAGGC 
GACTCTGGGA GGAAGGAGGT CGAGTCTCTG TTTGATTTCT TCCAGGAAAA AGATGACGAT 
TTGCACAAAT CCTACAACGA CAGATCGTAC ATCAGCTCCG AGGACACCAG CACTCGAGAC 
AGCACCACCA CCGATTTTGT GGCGCTGCTG ACGGGTGTGA CAGACTCTTG GCTGCCGAGC 
TCCAGTGGTG TCGCGAGGGC CAGATTTACA CTTTCTCGAA CAAGTCTGAC TTTCTCCATC 
ACTTTCCAGA GAATAAACAG ACCGAGTCTC ATTGCATTTC TGGACACTGA CGGAAACACC 
GCGTTTGAGT TCAGAGTCCC GCAGGCCGAT AACGACATGA TCTGCGGCAT CTGGAAAAAT 
GTGCCGAAAC CTCACATGCG GCAACTGGAG GCGGAGCAAC TGCACGTCTC CATGACCACC 
GCTGACAACA GGAAGGAGGA GCTTCAGGGC AGAATAATCA AACACAGAGC ACTGTTTGCT 
GAAACGTTCA GTGCAATCCT GACATCTGAC GAGGTGCATT CTGGGATGGG TGGAATCGCC 
ATGTTGACGC TCAGTGACAC CGAAAACAAT CTGCATTTTA TCCTGATTAT GCAGGGACTC 
GTTCCTCCAG GGAGCTCCAA GGTGCCGGTG CGAGTGAAAC TGCAGTATCG ACAACACCTG 
CTGAGAGAAA TACGAGCAAA CATCACTGCA GACGACTCAG ACTTTGCTGA AGTGCTGGCG 
GATCTGAACA GTCGTGAGCT CTTCTGGTTG TCCCGCGGGC AGCTCCAGAT CTCCGTCCAG 
ACTGAAGGTC AAACCCCGCG TCACATCTCG GGATTCATTT CTGGAAGGAG ATCATGTGAC 
ACTCTGCAGA GTGTGTTGTC GAGCGGCGCG GCTCTGACTG CGGGTCAGAC GGGCGGTGTG 
GGCTCTGCTG TCTTCACTCT GCACCCGAAC GGCTCTCTGG ATTACCAGCT GCTGGTGGCG 
GGTCTGAGCA GCGCGGTGCT GAGCGTTAGC ATCGAGATGA AGCCGCGTCG GCGCAACAAA 
CGCAGCGTTC TGTACGAGCT CTCGGCAGTG TTCACAGACC AGCGGGCGGC AGGCAGCTGC 
GGACGCGTGG AGGCCAGACA CACACACATG CTGCTGCAGA ACGAGCTGTT CATCAACATC 
GCCACCGCAC TGCAGCCCGA CGGGGAGCTG CGCGGACAGA TACGCCTGCT GCCATACAAT 
GGACTGGACG CGCGCAGAAA CGAGTTGCCG GTTCCTCTGG CCGGTGTTCT GGTGTCTCCG 
CCGGTGCGCA CTGGTGCAGC GGGTCACGCC TGGGTCTCGG TGGACCCTCA GTGTCATCTG 
CATTACGAGA TCATCGTTAA CGGACTCAGC AAGAGCGAAG ACGCCTCCAT CAGTGCTCAC 
CTGCATGGAT TGGCTGAGAT CGGAGAGATG GACGACTCTT CCACCAATCA CAAGAGACTC 
CTGACTGGTT TCTACGGGCA ACAGGCTCAA GGCGTCCTGA AGGACATCAG TGTTGAATTA 
TTGCGTCATT TAAATGAAGG TACGGCGTAT CTGCAGGTCA GCACCAAGAT GAACCCCAGA 
GGAGAGATTC GCGGACGGAT CCACGTCCCG AACCACTGTG AGTCCCCGGC GCCCCGCGCT 
GAGTTTCTGG AGGAGCCGGA GTTTGAGGAT CTGCTGTTCA CACGGGAGCC GACGGAGCTG 
CGCAAAGACA CACACACTCA CGTACACTCC TGCTTCTTTG AGGGAGAACA ACACACACAC 
GGCTCACAGT GGACACCGCA GTACAACACC TGCTTCACCT GCACCTGCCA GAAAAAGACG 
GTGATCTGTG ACCCAGTGAT GTGTCCTACA CTCTCCTGCA CACACACCGT CCAGCCTGAA 
GACCAATGCT GTCCCATCTG TGAAGAGAAG AAAGAATCTA AAGAGACAGC CGCTGTAGAA 
AAAGTTGAGG AAAATCCTGA AGGCTGTTAT TTCGAAGGCG ATCAGAAAAT GCACGCACCT 
GGAACAACAT GGCATCCGTT CGTCCCGCCG TTCGGCTACA TCAAGTGTGC AGTCTGTACC 
TGCAAGGGCT CGACAGGGGA AGTGCACTGC GAGAAGGTGA CGTGCCCGCC CCTCACCTGC 
AGCCGACCAA TCAGACGAAA CCCTTCAGAC TGCTGTAAGG AGTGTCCTCC TGAAGAAACG 
CCCCCTCTGG AGGACGAGGA GATGATGCAG GCGGACGGGA CGCGGCTCTG CAAATTCGGG 
AAAAACTACT ATCAGAACAG CGAACACTGG CACCCGAGCG TCCCGCTGGT GGGGGAGATG 
AAGTGCATCA CCTGCTGGTG TGACCATGGC GTCACAAAGT GTCAGAGGAA GCAGTGTCCA 
CTGCTGAGCT GCAGAAATCC CATCCGCACA GAGGGAAAAT GCTGTCCGGA ATGCATAGAG 
GACTTCATGG AGAAAGAAGA AATGGCAAAG ATGGCAGAGA AAAAGAAAAG CTGGAGACAC 
TGA