ube4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube4b
Entrez Gene ID	170784
Full Name	ubiquitination factor E4B, UFD2 homolog (S. cerevisiae)
Synonyms	UFD2,zgc:109766
General protein information	Preferred Names ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) Names ubiquitin conjugation factor E4 B UBE4B-II splice UBE4B-III splice isoform III
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_194362.2	NP_919343.2	ubiquitin conjugation factor E4 B
XM_021469719.1	XP_021325394.1	ubiquitin conjugation factor E4 B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells.
Mammen AL, Mahoney JA, St Germain A, Badders N, Taylor JP, Rosen A, Spinette S
PloS one6(12)e28861(2011)

The human homologue of the yeast polyubiquitination factor Ufd2p is cleaved by caspase 6 and granzyme B during apoptosis.
Mahoney JA, Odin JA, White SM, Shaffer D, Koff A, Casciola-Rosen L, Rosen A
The Biochemical journal361(Pt 3)587-95(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The regulated expression of these UFD2a isoforms most likely imparts divergent functions that are important for myogenesis.
Title: A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells.

The following ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa151418	XM_021469719.1 Latest version!	Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa39541	NM_194362.2 Latest version!	Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube4b ( XM_021469719.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325394.1 Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151418
Clone ID Related Accession (Same CDS sequence)	XM_021469719.1
Accession Version	XM_021469719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TCAGTCCTGA AGAGATCCGT AGAAGGCGGT TGGCCCGGTT AGCTGGAGGC 
CAGACATCAC AGCCCAGCAC CCCCCTCAGC ACCCCTCTGA CATCCCCACA GAGAGAGACT 
CCCCCAGGAC CTTTGCCAGG ACCCTCCGGG GCCTCATCAC AGCCTGTCCC ACCAGCTCCA 
TCTCACACTC TCGCACTAAA TGCCCAAAAT GTCACCCCTG CCACCTCTCC TATGGGGGCC 
TCAGGGGTTG CGTACCGCAG CCAGAGCAGC GAGGGGGTCA GTTCTCTCTC CAGCTCTCCA 
TCTAACAGCC TGGAGACACA GTCCCAATCC CTATCCCGCT CCCAGAGCAT GGACATCGAC 
ACAGCCTCCT GCGAGAAGAG CATGTCCCAA GTAGATGTGG ACTCAGGAAT CGAGAATATG 
GAGGTGGAAG ACAGCGATCG CAGGGAGAAA AGGAACCTTG CTGAGAAGGA CTCCTCATCC 
AATCCCGATG TCTCTGAAGA GTTGGCGTTG CAGCTAATCT GTAAGATTTT GCGTGTTTCC 
TGGAAGGAGC AGGACCGTGA TGTCATCTTC CTCCCTTCTC TTGCTGCTGA GTTCCAGAAG 
AACCCTAAAG ATGTTTACTC TGACTTCAAG GACCTGATCG GGCAGATTTT GATGGAGGCT 
CTCATGATGT CCACCCGTTC CCGTGACTGT AACCCATTCG CCAGCTTGAC CGCCACATCT 
CAGCCAATTA CTGCTGCCAG ATCTCCAGAC CATCACCTGA CTCTTGTTCC ACCCTCCAGC 
CAGGGCGGCA GTCCCATGAT GCCCTGCGCT GGTTCATTCG GTGCCAGCTC TCTTTCCAGT 
CTGTATGGTT GTAGCCCAAA CCCACTAGCT CTGAGCTCTG CCAGATTGAG TGCTCCATCT 
GTCCCACCGG CTTCTCCAGC CGCTCTTCCA GTATCCCTCA GTCCGCCGAT CTCTCGCCCT 
CCCGCCAGCC TTCCATCTCC ATCCGCCCCT TTGCCCATCT CCCAGAGATA CAGGCCCTAC 
TCCCTTAGCT CATCCATTCC TATAAGCCCA AGCTCCAGAT CTGCTCCATC TGGTATGCTC 
ACTTCCCCTA CAACTTCAGA GACCCCCTCC AGGCCCTCTC CCAGACAACA TAGCACCCAA 
ACCTCTATGC CTTTCCCCTT TCCGCCGGGC TCCCCCACCA CTATGGCCAG AAGGACTGCA 
CTGGCCACCC GGATGCCCTC TAGCAGCCCT TTCTCCCTCC TCTTCTTCGC CTTGTCCGAC 
TTGCCTCAGG ACAGCAGTGA TGAAGAACAA GAGGAAGAAG GAGAGGAGGA TTTTTCAGGG 
GTTCAGTTTG GGTCCAGCCT GGGTGCATCT GCAGGAGGGA TGGCTGGTGA CTCAGGTAGT 
GACCGCTTTA CCATAGAGTC CTGTAAAGAG ACGGAGATGC TCAACTACCT CATCGAGCGC 
TTTGACAGCG TGGGGATGGA GGAGAGAAAG GCCCCAAAGA TGTGCAGCCA ACCAGCTGTC 
AGTCAGCTGC TGAGCAACAT TCGCTCCCAG TGCATTTCCC ATGCTGCACT AGTGCTTCAA 
GGTGCCCTCA CACAGCCCAG AGCCCCTCTT CAGCCCTCCC TGCTGGTTCC CTACATGCTG 
TGCCGGAACC TTCCATACGG TTTCATCCAG GAGCTGGTGC GCATGACTCA CCAGGAGGAG 
GACGTGTTCA AACAGATCTT CGTTCCCATT CTTCAAGGGC TTGCTTTAGC TGTAAAAGAA 
TGTTCTTTTG ACAGTGACAA CTTCAAGTTC CCTCTGATGG CTTTGGCCGA GCTCTGTGAA 
ATCAAGTTCG GGAAGACTCA CCCTGTATGC AATTTGATCA CCTCTCTGCC GTTGTGGTGT 
CCTGACCCTC TCAGTCCCGG GACAGGAAGA GAAATTCAAA GACTCTCCTT CCTCGGAGCG 
TTCTTCAGCC TTTCTGTGTT CGCTGAAGAT GACACTAAAG TGGGAGACAA GTACTTCTCA 
GGCCCTGCCA TCACTATGGA GAACACGCGT GTGGTCAGCC AGTCTCTGCA GCACTATCTG 
GAGTCTGCCA GGGGAGATCT GTTTAAAATC CTGCACAACA TCTTGCTGAA TGGAGAGACA 
AGGGAAGCAG CTCTGAGCTA CATGGCAGCT CTTGTGAATC GTAATGTGAA GAAGGCTCAG 
ATGCAGACCG ATGATAAGTT GGTGTCCACA GACGGCTTCA TGATGAACTT CCTGTGGGTC 
CTGCAGCAGC TCAGTATGAA GATCAAGCTG GAAACAGTGG ATCCTCTCTA TATCTTCCAC 
CCCAAATGTC GGCTGAATGT CAGCCCGGAA GAGACGCGGC TCAAAGCCAC TATGGAGGAA 
CTAAAGAGCT GGCTCACTGA GCTGCATGAA GACCCAACCA AGTTTTCAGA GCCCAAGTTC 
CCAACGGAGT GCTTTTTCTT AACGCTTCAC GCACATCATC TGTCCATCCT CCCATGCTGT 
CGGCGCTACA TAAGGAGACT GAGGGCCATC AGGGATCTGA ACAGAACTGT AGAGGAGCTG 
AAGAACAGTG AGAACCAGTG GAAGGATTCC CCGTTGGCTG GTCGTCATCG GGAGATGCTT 
AAGAGATGTA AAACACAGCT CAAGAAACTG GTGCGCTCTA AAGCGTGTGC AGATGCAGGG 
CTGCTGGATG AGAACCTCCT CCGCAGATGT CTGCAGTTCT TCAGTATGGT CATCCAGCTC 
ATCCTGCGTA TGGTGGAGCC TGCCTTCCCA CATGTATCCC TGCCTCTGAA TCCAGAAATC 
CCCAAAAGCT TTGCAGCACT GCCCGAGTTT TACATTGAAG ATGTGGCTGA GTTCATGCTT 
TTTATTGTGC AGTACTTTCC GCAGGTTCTC TATGAGCCGT GCACTGAAGA CATTGTGACC 
TTCCTGATAG TCTTCATTTG CAGTCAGAAT TATATAAAGA ACCCATACTT AATTGCCAAG 
CTGGTGGAGG TGTTGTTCGT CACCAACCCT GCGGTACAGC CGCGCACTCA GCGTTTCTTC 
GAGATGTTGG AGAACCACCC GCTGTCTGTC AATCAGCTTG TGCCCGCCCT TATGAAATTC 
TACACAGATG TGGAGCACAC TGGAGCCACA AGCGAGTTCT ATGACAAGTT CACCATTCGC 
TATCACATCA GCACCATTTT CAAAAGCCTC TGGCAAAACA TTAACCACCA AGGCACTTTC 
CTGGAGGAGT TCAACTCCGG TAAACAGTTT GTGCGCTACA TCAACATGTT AATAAATGAC 
ACAACGTTTC TGCTGGACGA GAGTTTGGAG TCTCTGAAAC GCATCCATGA GATTCAGGAG 
GAAATGAAGA ATAAGGAGCA ATGGGACTTG CTTCCCAGGG AGCAGCAGCA GAGCAGACAG 
TCTCAGTTGA CTCAGGATGA GCGAGTGTCA CGGTCTTACC TGGCTTTAGC CACAGAAACG 
GTGGATATGT TTCATATCCT CACCAAACAG GTCCAGAAGC CGTTCCTCAG GCCTGAACTG 
GGTCCTCGTC TGGCTGCCAT GTTGAATTAT AACCTGCAGC AGCTATGTGG GCCTAAATGT 
CGAGACTTGA AGGTGGAAAA CCCTGAAAAA TATGGTTTCG AGCCAAAAAA GCTTCTGGAT 
CAGCTCACTG ACATCTACCT GCAGCTAGAC TGTCCCCGAT TTGCTAAAGC CATCGCAGAT 
GACCAGCGGT CGTATAGCAG GGAGCTCTTT GAGGAGGTCA TTTCCAAAAT GAGAAAGGCG 
GGTATTAAAT CCACCATCGC TATTGAGAAA TTCAAGCTTC TGTTGGAGAA GGTGGAGGAG 
ATTGTAGCCC GCAATTCTCA GTCAGAGATG GATTACAGTG ACGCTCCGGA TGAGTTTAAA 
GACCCGCTGA TGGACACTCT CATGACCGAT CCCGTGCAAC TTCCCTCGGG CAACATAATG 
GACCGTTCCA TCATCCTGCG ACACCTGCTG AACTCTCCCA CTGACCCCTT CAACAGACAG 
CCTCTGACCG AGAGCATGCT GGAACCAGTC CCAGAGCTGA AGGAGCGAAT CCAGGCCTGG 
ATGAGAGAGA AGCAGACCGG CCGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325394.1
CDS	280..4329
Translation	MEQLSPEEIRRRRLARLAGGQTSQPSTPLSTPLTSPQRETPPGPLPGPSGASSQPVPPAPSHTLALNAQNVTPATSPMGASGVAYRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDTASCEKSMSQVDVDSGIENMEVEDSDRREKRNLAEKDSSSNPDVSEELALQLICKILRVSWKEQDRDVIFLPSLAAEFQKNPKDVYSDFKDLIGQILMEALMMSTRSRDCNPFASLTATSQPITAARSPDHHLTLVPPSSQGGSPMMPCAGSFGASSLSSLYGCSPNPLALSSARLSAPSVPPASPAALPVSLSPPISRPPASLPSPSAPLPISQRYRPYSLSSSIPISPSSRSAPSGMLTSPTTSETPSRPSPRQHSTQTSMPFPFPPGSPTTMARRTALATRMPSSSPFSLLFFALSDLPQDSSDEEQEEEGEEDFSGVQFGSSLGASAGGMAGDSGSDRFTIESCKETEMLNYLIERFDSVGMEERKAPKMCSQPAVSQLLSNIRSQCISHAALVLQGALTQPRAPLQPSLLVPYMLCRNLPYGFIQELVRMTHQEEDVFKQIFVPILQGLALAVKECSFDSDNFKFPLMALAELCEIKFGKTHPVCNLITSLPLWCPDPLSPGTGREIQRLSFLGAFFSLSVFAEDDTKVGDKYFSGPAITMENTRVVSQSLQHYLESARGDLFKILHNILLNGETREAALSYMAALVNRNVKKAQMQTDDKLVSTDGFMMNFLWVLQQLSMKIKLETVDPLYIFHPKCRLNVSPEETRLKATMEELKSWLTELHEDPTKFSEPKFPTECFFLTLHAHHLSILPCCRRYIRRLRAIRDLNRTVEELKNSENQWKDSPLAGRHREMLKRCKTQLKKLVRSKACADAGLLDENLLRRCLQFFSMVIQLILRMVEPAFPHVSLPLNPEIPKSFAALPEFYIEDVAEFMLFIVQYFPQVLYEPCTEDIVTFLIVFICSQNYIKNPYLIAKLVEVLFVTNPAVQPRTQRFFEMLENHPLSVNQLVPALMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNINHQGTFLEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEIQEEMKNKEQWDLLPREQQQSRQSQLTQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNYNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCPRFAKAIADDQRSYSRELFEEVISKMRKAGIKSTIAIEKFKLLLEKVEEIVARNSQSEMDYSDAPDEFKDPLMDTLMTDPVQLPSGNIMDRSIILRHLLNSPTDPFNRQPLTESMLEPVPELKERIQAWMREKQTGRY

Target ORF information:

RefSeq Version	XM_021469719.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469719.1

ORF Insert Sequence:

ATGGAGCAGC TCAGTCCTGA AGAGATCCGT AGAAGGCGGT TGGCCCGGTT AGCTGGAGGC 
CAGACATCAC AGCCCAGCAC CCCCCTCAGC ACCCCTCTGA CATCCCCACA GAGAGAGACT 
CCCCCAGGAC CTTTGCCAGG ACCCTCCGGG GCCTCATCAC AGCCTGTCCC ACCAGCTCCA 
TCTCACACTC TCGCACTAAA TGCCCAAAAT GTCACCCCTG CCACCTCTCC TATGGGGGCC 
TCAGGGGTTG CGTACCGCAG CCAGAGCAGC GAGGGGGTCA GTTCTCTCTC CAGCTCTCCA 
TCTAACAGCC TGGAGACACA GTCCCAATCC CTATCCCGCT CCCAGAGCAT GGACATCGAC 
ACAGCCTCCT GCGAGAAGAG CATGTCCCAA GTAGATGTGG ACTCAGGAAT CGAGAATATG 
GAGGTGGAAG ACAGCGATCG CAGGGAGAAA AGGAACCTTG CTGAGAAGGA CTCCTCATCC 
AATCCCGATG TCTCTGAAGA GTTGGCGTTG CAGCTAATCT GTAAGATTTT GCGTGTTTCC 
TGGAAGGAGC AGGACCGTGA TGTCATCTTC CTCCCTTCTC TTGCTGCTGA GTTCCAGAAG 
AACCCTAAAG ATGTTTACTC TGACTTCAAG GACCTGATCG GGCAGATTTT GATGGAGGCT 
CTCATGATGT CCACCCGTTC CCGTGACTGT AACCCATTCG CCAGCTTGAC CGCCACATCT 
CAGCCAATTA CTGCTGCCAG ATCTCCAGAC CATCACCTGA CTCTTGTTCC ACCCTCCAGC 
CAGGGCGGCA GTCCCATGAT GCCCTGCGCT GGTTCATTCG GTGCCAGCTC TCTTTCCAGT 
CTGTATGGTT GTAGCCCAAA CCCACTAGCT CTGAGCTCTG CCAGATTGAG TGCTCCATCT 
GTCCCACCGG CTTCTCCAGC CGCTCTTCCA GTATCCCTCA GTCCGCCGAT CTCTCGCCCT 
CCCGCCAGCC TTCCATCTCC ATCCGCCCCT TTGCCCATCT CCCAGAGATA CAGGCCCTAC 
TCCCTTAGCT CATCCATTCC TATAAGCCCA AGCTCCAGAT CTGCTCCATC TGGTATGCTC 
ACTTCCCCTA CAACTTCAGA GACCCCCTCC AGGCCCTCTC CCAGACAACA TAGCACCCAA 
ACCTCTATGC CTTTCCCCTT TCCGCCGGGC TCCCCCACCA CTATGGCCAG AAGGACTGCA 
CTGGCCACCC GGATGCCCTC TAGCAGCCCT TTCTCCCTCC TCTTCTTCGC CTTGTCCGAC 
TTGCCTCAGG ACAGCAGTGA TGAAGAACAA GAGGAAGAAG GAGAGGAGGA TTTTTCAGGG 
GTTCAGTTTG GGTCCAGCCT GGGTGCATCT GCAGGAGGGA TGGCTGGTGA CTCAGGTAGT 
GACCGCTTTA CCATAGAGTC CTGTAAAGAG ACGGAGATGC TCAACTACCT CATCGAGCGC 
TTTGACAGCG TGGGGATGGA GGAGAGAAAG GCCCCAAAGA TGTGCAGCCA ACCAGCTGTC 
AGTCAGCTGC TGAGCAACAT TCGCTCCCAG TGCATTTCCC ATGCTGCACT AGTGCTTCAA 
GGTGCCCTCA CACAGCCCAG AGCCCCTCTT CAGCCCTCCC TGCTGGTTCC CTACATGCTG 
TGCCGGAACC TTCCATACGG TTTCATCCAG GAGCTGGTGC GCATGACTCA CCAGGAGGAG 
GACGTGTTCA AACAGATCTT CGTTCCCATT CTTCAAGGGC TTGCTTTAGC TGTAAAAGAA 
TGTTCTTTTG ACAGTGACAA CTTCAAGTTC CCTCTGATGG CTTTGGCCGA GCTCTGTGAA 
ATCAAGTTCG GGAAGACTCA CCCTGTATGC AATTTGATCA CCTCTCTGCC GTTGTGGTGT 
CCTGACCCTC TCAGTCCCGG GACAGGAAGA GAAATTCAAA GACTCTCCTT CCTCGGAGCG 
TTCTTCAGCC TTTCTGTGTT CGCTGAAGAT GACACTAAAG TGGGAGACAA GTACTTCTCA 
GGCCCTGCCA TCACTATGGA GAACACGCGT GTGGTCAGCC AGTCTCTGCA GCACTATCTG 
GAGTCTGCCA GGGGAGATCT GTTTAAAATC CTGCACAACA TCTTGCTGAA TGGAGAGACA 
AGGGAAGCAG CTCTGAGCTA CATGGCAGCT CTTGTGAATC GTAATGTGAA GAAGGCTCAG 
ATGCAGACCG ATGATAAGTT GGTGTCCACA GACGGCTTCA TGATGAACTT CCTGTGGGTC 
CTGCAGCAGC TCAGTATGAA GATCAAGCTG GAAACAGTGG ATCCTCTCTA TATCTTCCAC 
CCCAAATGTC GGCTGAATGT CAGCCCGGAA GAGACGCGGC TCAAAGCCAC TATGGAGGAA 
CTAAAGAGCT GGCTCACTGA GCTGCATGAA GACCCAACCA AGTTTTCAGA GCCCAAGTTC 
CCAACGGAGT GCTTTTTCTT AACGCTTCAC GCACATCATC TGTCCATCCT CCCATGCTGT 
CGGCGCTACA TAAGGAGACT GAGGGCCATC AGGGATCTGA ACAGAACTGT AGAGGAGCTG 
AAGAACAGTG AGAACCAGTG GAAGGATTCC CCGTTGGCTG GTCGTCATCG GGAGATGCTT 
AAGAGATGTA AAACACAGCT CAAGAAACTG GTGCGCTCTA AAGCGTGTGC AGATGCAGGG 
CTGCTGGATG AGAACCTCCT CCGCAGATGT CTGCAGTTCT TCAGTATGGT CATCCAGCTC 
ATCCTGCGTA TGGTGGAGCC TGCCTTCCCA CATGTATCCC TGCCTCTGAA TCCAGAAATC 
CCCAAAAGCT TTGCAGCACT GCCCGAGTTT TACATTGAAG ATGTGGCTGA GTTCATGCTT 
TTTATTGTGC AGTACTTTCC GCAGGTTCTC TATGAGCCGT GCACTGAAGA CATTGTGACC 
TTCCTGATAG TCTTCATTTG CAGTCAGAAT TATATAAAGA ACCCATACTT AATTGCCAAG 
CTGGTGGAGG TGTTGTTCGT CACCAACCCT GCGGTACAGC CGCGCACTCA GCGTTTCTTC 
GAGATGTTGG AGAACCACCC GCTGTCTGTC AATCAGCTTG TGCCCGCCCT TATGAAATTC 
TACACAGATG TGGAGCACAC TGGAGCCACA AGCGAGTTCT ATGACAAGTT CACCATTCGC 
TATCACATCA GCACCATTTT CAAAAGCCTC TGGCAAAACA TTAACCACCA AGGCACTTTC 
CTGGAGGAGT TCAACTCCGG TAAACAGTTT GTGCGCTACA TCAACATGTT AATAAATGAC 
ACAACGTTTC TGCTGGACGA GAGTTTGGAG TCTCTGAAAC GCATCCATGA GATTCAGGAG 
GAAATGAAGA ATAAGGAGCA ATGGGACTTG CTTCCCAGGG AGCAGCAGCA GAGCAGACAG 
TCTCAGTTGA CTCAGGATGA GCGAGTGTCA CGGTCTTACC TGGCTTTAGC CACAGAAACG 
GTGGATATGT TTCATATCCT CACCAAACAG GTCCAGAAGC CGTTCCTCAG GCCTGAACTG 
GGTCCTCGTC TGGCTGCCAT GTTGAATTAT AACCTGCAGC AGCTATGTGG GCCTAAATGT 
CGAGACTTGA AGGTGGAAAA CCCTGAAAAA TATGGTTTCG AGCCAAAAAA GCTTCTGGAT 
CAGCTCACTG ACATCTACCT GCAGCTAGAC TGTCCCCGAT TTGCTAAAGC CATCGCAGAT 
GACCAGCGGT CGTATAGCAG GGAGCTCTTT GAGGAGGTCA TTTCCAAAAT GAGAAAGGCG 
GGTATTAAAT CCACCATCGC TATTGAGAAA TTCAAGCTTC TGTTGGAGAA GGTGGAGGAG 
ATTGTAGCCC GCAATTCTCA GTCAGAGATG GATTACAGTG ACGCTCCGGA TGAGTTTAAA 
GACCCGCTGA TGGACACTCT CATGACCGAT CCCGTGCAAC TTCCCTCGGG CAACATAATG 
GACCGTTCCA TCATCCTGCG ACACCTGCTG AACTCTCCCA CTGACCCCTT CAACAGACAG 
CCTCTGACCG AGAGCATGCT GGAACCAGTC CCAGAGCTGA AGGAGCGAAT CCAGGCCTGG 
ATGAGAGAGA AGCAGACCGG CCGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( NM_194362.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919343.2 Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), mRNA.

Price & Availability↑

CloneID	ODa39541
Clone ID Related Accession (Same CDS sequence)	NM_194362.2
Accession Version	NM_194362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ubiquitin conjugation factor E4 B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571850.7, EH441473.1, EH459986.1 and EH552968.1. On Jun 26, 2015 this sequence version replaced NM_194362.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01004662.1, GFIL01042580.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCAGC TCAGTCCTGA AGAGATCCGT AGAAGGCGGT TGGCCCGGTT AGCTGGAGGC 
CAGACATCAC AGCCCAGCAC CCCCCTCAGC ACCCCTCTGA CATCCCCACA GAGAGAGACT 
CCCCCAGGAC CTTTGCCAGG ACCCTCCGGG GCCTCATCAC AGCCTGTCCC ACCAGCTCCA 
TCTCACACTC TCGCACTAAA TGCCCAAAAT GTCACCCCTG CCACCTCTCC TATGGGGGCC 
TCAGGGGTTG CGTACCGCAG CCAGAGCAGC GAGGGGGTCA GTTCTCTCTC CAGCTCTCCA 
TCTAACAGCC TGGAGACACA GTCCCAATCC CTATCCCGCT CCCAGAGCAT GGACATCGAC 
ACAGCCTCCT GCGAGAAGAG CATGTCCCAA GTAGATGTGG ACTCAGGAAT CGAGAATATG 
GAGGTGGAAG ACAGCGATCG CAGGGAGAAA AGGAACCTTG CTGAGAAGGA CTCCTCATCC 
AATCCCGATG TCTCTGAAGA GTTGGCGTTG CAGCTAATCT GTAAGATTTT GCGTGTTTCC 
TGGAAGGAGC AGGACCGTGA TGTCATCTTC CTCCCTTCTC TTGCTGCTGA GTTCCAGAAG 
AACCCTAAAG ATGTTTACTC TGACTTCAAG GACCTGATCG GGCAGATTTT GATGGAGGCT 
CTCATGATGT CCACCCGTTC CCGTGACTGT AACCCATTCG CCAGCTTGAC CGCCACATCT 
CAGCCAATTA CTGCTGCCAG ATCTCCAGAC CATCACCTGA CTCTTGTTCC ACCCTCCAGC 
CAGGGCGGCA GTCCCATGAT GCCCTGCGCT GGTTCATTCG GTGCCAGCTC TCTTTCCAGC 
CTGGGTGCAT CTGCAGGAGG GATGGCTGGT GACTCAGGTA GTGACCGCTT TACCATAGAG 
TCCTGTAAAG AGACGGAGAT GCTCAACTAC CTCATCGAGC GCTTTGACAG CGTGGGGATG 
GAGGAGAGAA AGGCCCCAAA GATGTGCAGC CAACCAGCTG TCAGTCAGCT GCTGAGCAAC 
ATTCGCTCCC AGTGCATTTC CCATGCTGCA CTAGTGCTTC AAGGTGCCCT CACACAGCCC 
AGAGCCCCTC TTCAGCCCTC CCTGCTGGTT CCCTACATGC TGTGCCGGAA CCTTCCATAC 
GGTTTCATCC AGGAGCTGGT GCGCATGACT CACCAGGAGG AGGACGTGTT CAAACAGATC 
TTCGTTCCCA TTCTTCAAGG GCTTGCTTTA GCTGTAAAAG AATGTTCTTT TGACAGTGAC 
AACTTCAAGT TCCCTCTGAT GGCTTTGGCC GAGCTCTGTG AAATCAAGTT CGGGAAGACT 
CACCCTGTAT GCAATTTGAT CACCTCTCTG CCGTTGTGGT GTCCTGACCC TCTCAGTCCC 
GGGACAGGAA GAGAAATTCA AAGACTCTCC TTCCTCGGAG CGTTCTTCAG CCTTTCTGTG 
TTCGCTGAAG ATGACACTAA AGTGGGAGAC AAGTACTTCT CAGGCCCTGC CATCACTATG 
GAGAACACGC GTGTGGTCAG CCAGTCTCTG CAGCACTATC TGGAGTCTGC CAGGGGAGAT 
CTGTTTAAAA TCCTGCACAA CATCTTGCTG AATGGAGAGA CAAGGGAAGC AGCTCTGAGC 
TACATGGCAG CTCTTGTGAA TCGTAATGTG AAGAAGGCTC AGATGCAGAC CGATGATAAG 
TTGGTGTCCA CAGACGGCTT CATGATGAAC TTCCTGTGGG TCCTGCAGCA GCTCAGTATG 
AAGATCAAGC TGGAAACAGT GGATCCTCTC TATATCTTCC ACCCCAAATG TCGGCTGAAT 
GTCAGCCCGG AAGAGACGCG GCTCAAAGCC ACTATGGAGG AACTAAAGAG CTGGCTCACT 
GAGCTGCATG AAGACCCAAC CAAGTTTTCA GAGCCCAAGT TCCCAACGGA GTGCTTTTTC 
TTAACGCTTC ACGCACATCA TCTGTCCATC CTCCCATGCT GTCGGCGCTA CATAAGGAGA 
CTGAGGGCCA TCAGGGATCT GAACAGAACT GTAGAGGAGC TGAAGAACAG TGAGAACCAG 
TGGAAGGATT CCCCGTTGGC TGGTCGTCAT CGGGAGATGC TTAAGAGATG TAAAACACAG 
CTCAAGAAAC TGGTGCGCTC TAAAGCGTGT GCAGATGCAG GGCTGCTGGA TGAGAACCTC 
CTCCGCAGAT GTCTGCAGTT CTTCAGTATG GTCATCCAGC TCATCCTGCG TATGGTGGAG 
CCTGCCTTCC CACATGTATC CCTGCCTCTG AATCCAGAAA TCCCCAAAAG CTTTGCAGCA 
CTGCCCGAGT TTTACATTGA AGATGTGGCT GAGTTCATGC TTTTTATTGT GCAGTACTTT 
CCGCAGGTTC TCTATGAGCC GTGCACTGAA GACATTGTGA CCTTCCTGAT AGTCTTCATT 
TGCAGTCAGA ATTATATAAA GAACCCATAC TTAATTGCCA AGCTGGTGGA GGTGTTGTTC 
GTCACCAACC CTGCGGTACA GCCGCGCACT CAGCGTTTCT TCGAGATGTT GGAGAACCAC 
CCGCTGTCTG TCAATCAGCT TGTGCCCGCC CTTATGAAAT TCTACACAGA TGTGGAGCAC 
ACTGGAGCCA CAAGCGAGTT CTATGACAAG TTCACCATTC GCTATCACAT CAGCACCATT 
TTCAAAAGCC TCTGGCAAAA CATTAACCAC CAAGGCACTT TCCTGGAGGA GTTCAACTCC 
GGTAAACAGT TTGTGCGCTA CATCAACATG TTAATAAATG ACACAACGTT TCTGCTGGAC 
GAGAGTTTGG AGTCTCTGAA ACGCATCCAT GAGATTCAGG AGGAAATGAA GAATAAGGAG 
CAATGGGACT TGCTTCCCAG GGAGCAGCAG CAGAGCAGAC AGTCTCAGTT GACTCAGGAT 
GAGCGAGTGT CACGGTCTTA CCTGGCTTTA GCCACAGAAA CGGTGGATAT GTTTCATATC 
CTCACCAAAC AGGTCCAGAA GCCGTTCCTC AGGCCTGAAC TGGGTCCTCG TCTGGCTGCC 
ATGTTGAATT ATAACCTGCA GCAGCTATGT GGGCCTAAAT GTCGAGACTT GAAGGTGGAA 
AACCCTGAAA AATATGGTTT CGAGCCAAAA AAGCTTCTGG ATCAGCTCAC TGACATCTAC 
CTGCAGCTAG ACTGTCCCCG ATTTGCTAAA GCCATCGCAG ATGACCAGCG GTCGTATAGC 
AGGGAGCTCT TTGAGGAGGT CATTTCCAAA ATGAGAAAGG CGGGTATTAA ATCCACCATC 
GCTATTGAGA AATTCAAGCT TCTGTTGGAG AAGGTGGAGG AGATTGTAGC CCGCAATTCT 
CAGTCAGAGA TGGATTACAG TGACGCTCCG GATGAGTTTA AAGACCCGCT GATGGACACT 
CTCATGACCG ATCCCGTGCA ACTTCCCTCG GGCAACATAA TGGACCGTTC CATCATCCTG 
CGACACCTGC TGAACTCTCC CACTGACCCC TTCAACAGAC AGCCTCTGAC CGAGAGCATG 
CTGGAACCAG TCCCAGAGCT GAAGGAGCGA ATCCAGGCCT GGATGAGAGA GAAGCAGACC 
GGCCGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919343.2
CDS	283..3834
Translation	MEQLSPEEIRRRRLARLAGGQTSQPSTPLSTPLTSPQRETPPGPLPGPSGASSQPVPPAPSHTLALNAQNVTPATSPMGASGVAYRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDTASCEKSMSQVDVDSGIENMEVEDSDRREKRNLAEKDSSSNPDVSEELALQLICKILRVSWKEQDRDVIFLPSLAAEFQKNPKDVYSDFKDLIGQILMEALMMSTRSRDCNPFASLTATSQPITAARSPDHHLTLVPPSSQGGSPMMPCAGSFGASSLSSLGASAGGMAGDSGSDRFTIESCKETEMLNYLIERFDSVGMEERKAPKMCSQPAVSQLLSNIRSQCISHAALVLQGALTQPRAPLQPSLLVPYMLCRNLPYGFIQELVRMTHQEEDVFKQIFVPILQGLALAVKECSFDSDNFKFPLMALAELCEIKFGKTHPVCNLITSLPLWCPDPLSPGTGREIQRLSFLGAFFSLSVFAEDDTKVGDKYFSGPAITMENTRVVSQSLQHYLESARGDLFKILHNILLNGETREAALSYMAALVNRNVKKAQMQTDDKLVSTDGFMMNFLWVLQQLSMKIKLETVDPLYIFHPKCRLNVSPEETRLKATMEELKSWLTELHEDPTKFSEPKFPTECFFLTLHAHHLSILPCCRRYIRRLRAIRDLNRTVEELKNSENQWKDSPLAGRHREMLKRCKTQLKKLVRSKACADAGLLDENLLRRCLQFFSMVIQLILRMVEPAFPHVSLPLNPEIPKSFAALPEFYIEDVAEFMLFIVQYFPQVLYEPCTEDIVTFLIVFICSQNYIKNPYLIAKLVEVLFVTNPAVQPRTQRFFEMLENHPLSVNQLVPALMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNINHQGTFLEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEIQEEMKNKEQWDLLPREQQQSRQSQLTQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNYNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCPRFAKAIADDQRSYSRELFEEVISKMRKAGIKSTIAIEKFKLLLEKVEEIVARNSQSEMDYSDAPDEFKDPLMDTLMTDPVQLPSGNIMDRSIILRHLLNSPTDPFNRQPLTESMLEPVPELKERIQAWMREKQTGRY

Target ORF information:

RefSeq Version	NM_194362.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (ube4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194362.2

ORF Insert Sequence:

ATGGAGCAGC TCAGTCCTGA AGAGATCCGT AGAAGGCGGT TGGCCCGGTT AGCTGGAGGC 
CAGACATCAC AGCCCAGCAC CCCCCTCAGC ACCCCTCTGA CATCCCCACA GAGAGAGACT 
CCCCCAGGAC CTTTGCCAGG ACCCTCCGGG GCCTCATCAC AGCCTGTCCC ACCAGCTCCA 
TCTCACACTC TCGCACTAAA TGCCCAAAAT GTCACCCCTG CCACCTCTCC TATGGGGGCC 
TCAGGGGTTG CGTACCGCAG CCAGAGCAGC GAGGGGGTCA GTTCTCTCTC CAGCTCTCCA 
TCTAACAGCC TGGAGACACA GTCCCAATCC CTATCCCGCT CCCAGAGCAT GGACATCGAC 
ACAGCCTCCT GCGAGAAGAG CATGTCCCAA GTAGATGTGG ACTCAGGAAT CGAGAATATG 
GAGGTGGAAG ACAGCGATCG CAGGGAGAAA AGGAACCTTG CTGAGAAGGA CTCCTCATCC 
AATCCCGATG TCTCTGAAGA GTTGGCGTTG CAGCTAATCT GTAAGATTTT GCGTGTTTCC 
TGGAAGGAGC AGGACCGTGA TGTCATCTTC CTCCCTTCTC TTGCTGCTGA GTTCCAGAAG 
AACCCTAAAG ATGTTTACTC TGACTTCAAG GACCTGATCG GGCAGATTTT GATGGAGGCT 
CTCATGATGT CCACCCGTTC CCGTGACTGT AACCCATTCG CCAGCTTGAC CGCCACATCT 
CAGCCAATTA CTGCTGCCAG ATCTCCAGAC CATCACCTGA CTCTTGTTCC ACCCTCCAGC 
CAGGGCGGCA GTCCCATGAT GCCCTGCGCT GGTTCATTCG GTGCCAGCTC TCTTTCCAGC 
CTGGGTGCAT CTGCAGGAGG GATGGCTGGT GACTCAGGTA GTGACCGCTT TACCATAGAG 
TCCTGTAAAG AGACGGAGAT GCTCAACTAC CTCATCGAGC GCTTTGACAG CGTGGGGATG 
GAGGAGAGAA AGGCCCCAAA GATGTGCAGC CAACCAGCTG TCAGTCAGCT GCTGAGCAAC 
ATTCGCTCCC AGTGCATTTC CCATGCTGCA CTAGTGCTTC AAGGTGCCCT CACACAGCCC 
AGAGCCCCTC TTCAGCCCTC CCTGCTGGTT CCCTACATGC TGTGCCGGAA CCTTCCATAC 
GGTTTCATCC AGGAGCTGGT GCGCATGACT CACCAGGAGG AGGACGTGTT CAAACAGATC 
TTCGTTCCCA TTCTTCAAGG GCTTGCTTTA GCTGTAAAAG AATGTTCTTT TGACAGTGAC 
AACTTCAAGT TCCCTCTGAT GGCTTTGGCC GAGCTCTGTG AAATCAAGTT CGGGAAGACT 
CACCCTGTAT GCAATTTGAT CACCTCTCTG CCGTTGTGGT GTCCTGACCC TCTCAGTCCC 
GGGACAGGAA GAGAAATTCA AAGACTCTCC TTCCTCGGAG CGTTCTTCAG CCTTTCTGTG 
TTCGCTGAAG ATGACACTAA AGTGGGAGAC AAGTACTTCT CAGGCCCTGC CATCACTATG 
GAGAACACGC GTGTGGTCAG CCAGTCTCTG CAGCACTATC TGGAGTCTGC CAGGGGAGAT 
CTGTTTAAAA TCCTGCACAA CATCTTGCTG AATGGAGAGA CAAGGGAAGC AGCTCTGAGC 
TACATGGCAG CTCTTGTGAA TCGTAATGTG AAGAAGGCTC AGATGCAGAC CGATGATAAG 
TTGGTGTCCA CAGACGGCTT CATGATGAAC TTCCTGTGGG TCCTGCAGCA GCTCAGTATG 
AAGATCAAGC TGGAAACAGT GGATCCTCTC TATATCTTCC ACCCCAAATG TCGGCTGAAT 
GTCAGCCCGG AAGAGACGCG GCTCAAAGCC ACTATGGAGG AACTAAAGAG CTGGCTCACT 
GAGCTGCATG AAGACCCAAC CAAGTTTTCA GAGCCCAAGT TCCCAACGGA GTGCTTTTTC 
TTAACGCTTC ACGCACATCA TCTGTCCATC CTCCCATGCT GTCGGCGCTA CATAAGGAGA 
CTGAGGGCCA TCAGGGATCT GAACAGAACT GTAGAGGAGC TGAAGAACAG TGAGAACCAG 
TGGAAGGATT CCCCGTTGGC TGGTCGTCAT CGGGAGATGC TTAAGAGATG TAAAACACAG 
CTCAAGAAAC TGGTGCGCTC TAAAGCGTGT GCAGATGCAG GGCTGCTGGA TGAGAACCTC 
CTCCGCAGAT GTCTGCAGTT CTTCAGTATG GTCATCCAGC TCATCCTGCG TATGGTGGAG 
CCTGCCTTCC CACATGTATC CCTGCCTCTG AATCCAGAAA TCCCCAAAAG CTTTGCAGCA 
CTGCCCGAGT TTTACATTGA AGATGTGGCT GAGTTCATGC TTTTTATTGT GCAGTACTTT 
CCGCAGGTTC TCTATGAGCC GTGCACTGAA GACATTGTGA CCTTCCTGAT AGTCTTCATT 
TGCAGTCAGA ATTATATAAA GAACCCATAC TTAATTGCCA AGCTGGTGGA GGTGTTGTTC 
GTCACCAACC CTGCGGTACA GCCGCGCACT CAGCGTTTCT TCGAGATGTT GGAGAACCAC 
CCGCTGTCTG TCAATCAGCT TGTGCCCGCC CTTATGAAAT TCTACACAGA TGTGGAGCAC 
ACTGGAGCCA CAAGCGAGTT CTATGACAAG TTCACCATTC GCTATCACAT CAGCACCATT 
TTCAAAAGCC TCTGGCAAAA CATTAACCAC CAAGGCACTT TCCTGGAGGA GTTCAACTCC 
GGTAAACAGT TTGTGCGCTA CATCAACATG TTAATAAATG ACACAACGTT TCTGCTGGAC 
GAGAGTTTGG AGTCTCTGAA ACGCATCCAT GAGATTCAGG AGGAAATGAA GAATAAGGAG 
CAATGGGACT TGCTTCCCAG GGAGCAGCAG CAGAGCAGAC AGTCTCAGTT GACTCAGGAT 
GAGCGAGTGT CACGGTCTTA CCTGGCTTTA GCCACAGAAA CGGTGGATAT GTTTCATATC 
CTCACCAAAC AGGTCCAGAA GCCGTTCCTC AGGCCTGAAC TGGGTCCTCG TCTGGCTGCC 
ATGTTGAATT ATAACCTGCA GCAGCTATGT GGGCCTAAAT GTCGAGACTT GAAGGTGGAA 
AACCCTGAAA AATATGGTTT CGAGCCAAAA AAGCTTCTGG ATCAGCTCAC TGACATCTAC 
CTGCAGCTAG ACTGTCCCCG ATTTGCTAAA GCCATCGCAG ATGACCAGCG GTCGTATAGC 
AGGGAGCTCT TTGAGGAGGT CATTTCCAAA ATGAGAAAGG CGGGTATTAA ATCCACCATC 
GCTATTGAGA AATTCAAGCT TCTGTTGGAG AAGGTGGAGG AGATTGTAGC CCGCAATTCT 
CAGTCAGAGA TGGATTACAG TGACGCTCCG GATGAGTTTA AAGACCCGCT GATGGACACT 
CTCATGACCG ATCCCGTGCA ACTTCCCTCG GGCAACATAA TGGACCGTTC CATCATCCTG 
CGACACCTGC TGAACTCTCC CACTGACCCC TTCAACAGAC AGCCTCTGAC CGAGAGCATG 
CTGGAACCAG TCCCAGAGCT GAAGGAGCGA ATCCAGGCCT GGATGAGAGA GAAGCAGACC 
GGCCGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells.
PloS one6(12)e28861(2011)
Mammen AL,Mahoney JA,St Germain A,Badders N,Taylor JP,Rosen A,Spinette S

The human homologue of the yeast polyubiquitination factor Ufd2p is cleaved by caspase 6 and granzyme B during apoptosis.
The Biochemical journal361(Pt 3)587-95(2002 Feb)
Mahoney JA,Odin JA,White SM,Shaffer D,Koff A,Casciola-Rosen L,Rosen A