ptpra cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptpra
Entrez Gene ID	140818
Full Name	protein tyrosine phosphatase, receptor type, A
Synonyms	RPTP[a]
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, A Names receptor-type tyrosine-protein phosphatase alpha protein-tyrosine phosphatase alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468747.1	XP_021324422.1	receptor-type tyrosine-protein phosphatase alpha isoform X1
NM_131888.2	NP_571963.2	receptor-type tyrosine-protein phosphatase alpha precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PTPRA	NP_001193428.1
Homo sapiens (human)	PTPRA	NP_002827.1
Mus musculus (house mouse)	Ptpra	NP_033006.2
Pan troglodytes (chimpanzee)	PTPRA	XP_001158174.2
	PTPRA	XP_002798264.1
Xenopus tropicalis (tropical clawed frog)	ptpra	NP_001121422.1
Canis lupus familiaris (dog)	PTPRA	XP_003433272.2
Rattus norvegicus (Norway rat)	Ptpra	NP_036895.2
Gallus gallus (chicken)	PTPRA	NP_990485.1
Danio rerio (zebrafish)	ptpra	NP_571963.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Wu W, Hale AJ, Lemeer S, den Hertog J
Scientific reports7(1)8460(2017 Aug)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebrafish as a model to study PTPs during development.
Paardekooper Overman J, den Hertog J
Methods (San Diego, Calif.)65(2)247-53(2014 Jan)

Identification and expression of the family of classical protein-tyrosine phosphatases in zebrafish.
van Eekelen M, Overvoorde J, van Rooijen C, den Hertog J
PloS one5(9)e12573(2010 Sep)

RPTPalpha and PTPepsilon signaling via Fyn/Yes and RhoA is essential for zebrafish convergence and extension cell movements during gastrulation.
van Eekelen M, Runtuwene V, Overvoorde J, den Hertog J
Developmental biology340(2)626-39(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ptpra protein, zebrafish, signaling is essential for convergence and extension cell movements during gastrulation.
Title: RPTPalpha and PTPepsilon signaling via Fyn/Yes and RhoA is essential for zebrafish convergence and extension cell movements during gastrulation.

The following ptpra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptpra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53183	NM_131888.2 Latest version!	Danio rerio protein tyrosine phosphatase receptor type A (ptpra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150833	XM_021468747.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, A (ptpra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptpra ( NM_131888.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571963.2 Danio rerio protein tyrosine phosphatase receptor type A (ptpra), mRNA.

Price & Availability↑

CloneID	ODa53183
Clone ID Related Accession (Same CDS sequence)	NM_131888.2
Accession Version	NM_131888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase alpha precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR847785.15 and EB928445.1. On Sep 1, 2015 this sequence version replaced NM_131888.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: Y15874.2, BC163526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505372, SAMEA3505393 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGTTT CGTTTCTCCA GGGCAGTATG GGTGTGTGTC CCCTGCTCCT GCATGTGGCC 
TTTGTGGCCC TCAGCACGGC CCAGACTCTC CCTCACCTTT CAGGTGCGGT CCAGACCACC 
AAACCTACAC CCCATCCTCC CACGACACCC CTAATCATAG TCACCAATGG ATCCCCAACA 
GCCGCTCCAA CATCCACACC GTCCCCACAA CTTATTGCAA CAAAAGCCTT CAATGACTCT 
GAGGGTACTG CGGCCCCAGA GGCTACCACT GCTCCTCCAG TCGTCCCTCC TCCAGCTGTG 
CCCATCCCTA CTGTGGTGCT ACCTGTACCG CCAACCCCAA CTGCCGCATC CCCGTCTCTC 
ACCACCAACA AAACTGGAGA TCACCAGACC ACCGTTTTGG CTCCAGCAGG GACGCCAACG 
CCTGATCCCA CATCCACCCA GTCCTCCTCA GAGCCCGAGA CGGAGACCCC GTATACCCCA 
GCTGATGCCA CCACTGCTGA CGTCAACAAG CCAGAAGGTG AAGAGCAAGA TGACACAGCG 
ATCATGGCAG TGATGGTTGC CTTATCCTCT CTGCTGGTCA TTGTCTTCAT CATCATCATC 
CTTTACATGC TCAGGTTTAA GAAGTACAAG CAGGCAGGGA GCCACTCCAA CTCCTTCCGC 
CTGACCAACG GCAGAGCTGA CGACACAGAA CTCCAGAGTG TACCGTTATT GGCTCGCTCT 
CCAAGCACCA ACAGGAAGTA CCCGCCTCTT CCTGTCGACA AGCTGGAGGA GGAAATGAAC 
CGTCGAATGG CTGACGACAA TAAACTCTTC CGGGAAGAAT TCAATGCGCT GCCGGTGTGT 
CCCATTCAAG CCTCATGTGA TGCCGCCTCC AAGGAGGAGA ATAAGGAGAA GAACAGATAT 
GTCAACATCT TGCCCTATGA TCATTCTAGA GTTCATTTGA CCTCTCTGGA GGGTGTTCCT 
GACTCAGACT ATATCAACGC TTCCTACATA AATGGATACC AGGAGAAAAA CAAATTCATT 
GCTGCACAAG GACCAAAAGA AGAGACAGTC AATGACTACT GGAGGATGAT CTGGGAGCAA 
AACACTGCCA CCATCGTCAT GGTCACCAAT CTGAAGGAGC GGAAAGAGTG TAAATGTGCT 
CAATACTGGC CTGACCAGGG ATGCTGGACT TACGGAAACA TCCGCGTGTC GGTAGAGGAC 
ACGATGATCT TGGTTGACTA CACCATCCGG AAGTTCTGCA TTCAGCAGGT GGGGGATGTT 
TCCGGTAAGA AGCCTCAGAG GCTGGTCACA CAGTTTCATT TCACCAGCTG GCCTGATTTT 
GGGGTTCCTT TCACTCCGAT TGGCATGCTG AAGTTCCTAA AGAAGGTCAA GAACTGCAAC 
CCACAGTATG CTGGACCCAT AATGGTTCAC TGCAGTGCGG GTGTTGGCAG GACGGGCACC 
TTTATCGTGA TAGACGCAAT GCTGGACATG ATGGGTGCAG AGAGAAAGGT GGACGTTTTC 
GGGTTTGTGA CGCGGATCAG AGCCCAGCGC TGTCAGATGG TTCAGACCGA TATGCAGTAT 
GTGTTTATTT TCCAAGCTTT GCTGGAGCAC TACCTGTACG GGGACACGGA GCTGGAAGTG 
ACGTCTCTGG AGACTCACAT GAATAAACTC TACGCTCCTT TACCTGGAGC TGGCTGTGGA 
GGCCTGGAGG CAGAGTTTAA GAAACTTACC TCCATTAAAA TCCAGAATGA CAAGATGAGA 
ACAGGAAACC TGCCTGCCAA CATGAAGAAG AATCGTGTTT TGCAAATCAT TCCATATGAG 
TTTAACAGAG TGATCATCCC TGTAAAACGT GGAGAGGAGA ACACGGATTA CATCAACGCC 
TCTTTTATAG ATGGTTACAG GCAGAAAGAC TCATACATGG CGTGTCAGGG GCCGCTCCAG 
CACACCATCG AGGACTACTG GAGGATGATA TGGGAGTGGA GGAGCTGCTC TATCGTTATG 
CTGACCGAGC TGGAGGAGAG AGGACAGGAG AAGTGTGCTC AGTATTGGCC TACAGATGGT 
GTGATGGTTT GTGGAGACAT GTCCATCGAG CTGAAGAGGG AGGAGGAAAG TGAGAGCTAC 
ACTGTTAGGG ACCTTCTAGT CACCAATAAC AGAGAAAATA AGTCTCGCGC TGTGAGGCAG 
TTTCATTTCC ATGGCTGGCC TGAGGTGGGC ATCCCGTCTG ATGGGAAGGG CATGATCAAC 
ATCATCGCTG CAGTTCAGAA GCAGCAGCAA CAGTCTGGAA ACCACCCAAT CACTGTGCAC 
TGCAGTGCTG GTGCAGGACG CACTGGGACA TTCTGTGCTC TCAGCACAGT CCTGGAGCGG 
GTGAAGGCAG AGGGCATTCT GGACGTTTTC CAGACAGTGA AGAGTCTAAG ACTACAGAGA 
CCGCACATGG TGCAGACACT GGAGCAGTAC GAGTTCTGCT ACAAAGTGGT CCAGGAATAC 
ATCGATGCCT TCTCTGACTA CGCCAACTTT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571963.2
CDS	366..2861
Translation	MQVSFLQGSMGVCPLLLHVAFVALSTAQTLPHLSGAVQTTKPTPHPPTTPLIIVTNGSPTAAPTSTPSPQLIATKAFNDSEGTAAPEATTAPPVVPPPAVPIPTVVLPVPPTPTAASPSLTTNKTGDHQTTVLAPAGTPTPDPTSTQSSSEPETETPYTPADATTADVNKPEGEEQDDTAIMAVMVALSSLLVIVFIIIILYMLRFKKYKQAGSHSNSFRLTNGRADDTELQSVPLLARSPSTNRKYPPLPVDKLEEEMNRRMADDNKLFREEFNALPVCPIQASCDAASKEENKEKNRYVNILPYDHSRVHLTSLEGVPDSDYINASYINGYQEKNKFIAAQGPKEETVNDYWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDTMILVDYTIRKFCIQQVGDVSGKKPQRLVTQFHFTSWPDFGVPFTPIGMLKFLKKVKNCNPQYAGPIMVHCSAGVGRTGTFIVIDAMLDMMGAERKVDVFGFVTRIRAQRCQMVQTDMQYVFIFQALLEHYLYGDTELEVTSLETHMNKLYAPLPGAGCGGLEAEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYINASFIDGYRQKDSYMACQGPLQHTIEDYWRMIWEWRSCSIVMLTELEERGQEKCAQYWPTDGVMVCGDMSIELKREEESESYTVRDLLVTNNRENKSRAVRQFHFHGWPEVGIPSDGKGMINIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

Target ORF information:

RefSeq Version	NM_131888.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase receptor type A (ptpra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131888.2

ORF Insert Sequence:

ATGCAGGTTT CGTTTCTCCA GGGCAGTATG GGTGTGTGTC CCCTGCTCCT GCATGTGGCC 
TTTGTGGCCC TCAGCACGGC CCAGACTCTC CCTCACCTTT CAGGTGCGGT CCAGACCACC 
AAACCTACAC CCCATCCTCC CACGACACCC CTAATCATAG TCACCAATGG ATCCCCAACA 
GCCGCTCCAA CATCCACACC GTCCCCACAA CTTATTGCAA CAAAAGCCTT CAATGACTCT 
GAGGGTACTG CGGCCCCAGA GGCTACCACT GCTCCTCCAG TCGTCCCTCC TCCAGCTGTG 
CCCATCCCTA CTGTGGTGCT ACCTGTACCG CCAACCCCAA CTGCCGCATC CCCGTCTCTC 
ACCACCAACA AAACTGGAGA TCACCAGACC ACCGTTTTGG CTCCAGCAGG GACGCCAACG 
CCTGATCCCA CATCCACCCA GTCCTCCTCA GAGCCCGAGA CGGAGACCCC GTATACCCCA 
GCTGATGCCA CCACTGCTGA CGTCAACAAG CCAGAAGGTG AAGAGCAAGA TGACACAGCG 
ATCATGGCAG TGATGGTTGC CTTATCCTCT CTGCTGGTCA TTGTCTTCAT CATCATCATC 
CTTTACATGC TCAGGTTTAA GAAGTACAAG CAGGCAGGGA GCCACTCCAA CTCCTTCCGC 
CTGACCAACG GCAGAGCTGA CGACACAGAA CTCCAGAGTG TACCGTTATT GGCTCGCTCT 
CCAAGCACCA ACAGGAAGTA CCCGCCTCTT CCTGTCGACA AGCTGGAGGA GGAAATGAAC 
CGTCGAATGG CTGACGACAA TAAACTCTTC CGGGAAGAAT TCAATGCGCT GCCGGTGTGT 
CCCATTCAAG CCTCATGTGA TGCCGCCTCC AAGGAGGAGA ATAAGGAGAA GAACAGATAT 
GTCAACATCT TGCCCTATGA TCATTCTAGA GTTCATTTGA CCTCTCTGGA GGGTGTTCCT 
GACTCAGACT ATATCAACGC TTCCTACATA AATGGATACC AGGAGAAAAA CAAATTCATT 
GCTGCACAAG GACCAAAAGA AGAGACAGTC AATGACTACT GGAGGATGAT CTGGGAGCAA 
AACACTGCCA CCATCGTCAT GGTCACCAAT CTGAAGGAGC GGAAAGAGTG TAAATGTGCT 
CAATACTGGC CTGACCAGGG ATGCTGGACT TACGGAAACA TCCGCGTGTC GGTAGAGGAC 
ACGATGATCT TGGTTGACTA CACCATCCGG AAGTTCTGCA TTCAGCAGGT GGGGGATGTT 
TCCGGTAAGA AGCCTCAGAG GCTGGTCACA CAGTTTCATT TCACCAGCTG GCCTGATTTT 
GGGGTTCCTT TCACTCCGAT TGGCATGCTG AAGTTCCTAA AGAAGGTCAA GAACTGCAAC 
CCACAGTATG CTGGACCCAT AATGGTTCAC TGCAGTGCGG GTGTTGGCAG GACGGGCACC 
TTTATCGTGA TAGACGCAAT GCTGGACATG ATGGGTGCAG AGAGAAAGGT GGACGTTTTC 
GGGTTTGTGA CGCGGATCAG AGCCCAGCGC TGTCAGATGG TTCAGACCGA TATGCAGTAT 
GTGTTTATTT TCCAAGCTTT GCTGGAGCAC TACCTGTACG GGGACACGGA GCTGGAAGTG 
ACGTCTCTGG AGACTCACAT GAATAAACTC TACGCTCCTT TACCTGGAGC TGGCTGTGGA 
GGCCTGGAGG CAGAGTTTAA GAAACTTACC TCCATTAAAA TCCAGAATGA CAAGATGAGA 
ACAGGAAACC TGCCTGCCAA CATGAAGAAG AATCGTGTTT TGCAAATCAT TCCATATGAG 
TTTAACAGAG TGATCATCCC TGTAAAACGT GGAGAGGAGA ACACGGATTA CATCAACGCC 
TCTTTTATAG ATGGTTACAG GCAGAAAGAC TCATACATGG CGTGTCAGGG GCCGCTCCAG 
CACACCATCG AGGACTACTG GAGGATGATA TGGGAGTGGA GGAGCTGCTC TATCGTTATG 
CTGACCGAGC TGGAGGAGAG AGGACAGGAG AAGTGTGCTC AGTATTGGCC TACAGATGGT 
GTGATGGTTT GTGGAGACAT GTCCATCGAG CTGAAGAGGG AGGAGGAAAG TGAGAGCTAC 
ACTGTTAGGG ACCTTCTAGT CACCAATAAC AGAGAAAATA AGTCTCGCGC TGTGAGGCAG 
TTTCATTTCC ATGGCTGGCC TGAGGTGGGC ATCCCGTCTG ATGGGAAGGG CATGATCAAC 
ATCATCGCTG CAGTTCAGAA GCAGCAGCAA CAGTCTGGAA ACCACCCAAT CACTGTGCAC 
TGCAGTGCTG GTGCAGGACG CACTGGGACA TTCTGTGCTC TCAGCACAGT CCTGGAGCGG 
GTGAAGGCAG AGGGCATTCT GGACGTTTTC CAGACAGTGA AGAGTCTAAG ACTACAGAGA 
CCGCACATGG TGCAGACACT GGAGCAGTAC GAGTTCTGCT ACAAAGTGGT CCAGGAATAC 
ATCGATGCCT TCTCTGACTA CGCCAACTTT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpra ( XM_021468747.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324422.1 Danio rerio protein tyrosine phosphatase, receptor type, A (ptpra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150833
Clone ID Related Accession (Same CDS sequence)	XM_021468747.1
Accession Version	XM_021468747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTT CGTTTCTCCA GGGCAGTATG GGTGTGTGTC CCCTGCTCCT GCATGTGGCC 
TTTGTGGCCC TCAGCACGGC CCAGACTCTC CCTCACCTTT CAGGTGCGGT CCAGACCACC 
AAACCTACAC CCCATCCTCC CACGACACCC CTAATCATAG TCACCAATGG ATCCCCAACA 
GCCGCTCCAA CATCCACACC GTCCCCACAA CTTATTGCAA CAAAAGCCTT CAATGACTCT 
GAGGGTACTG CGGCCCCAGA GGCTACCACT GCTCCTCCAG TCGTCCCTCC TCCAGCTGTG 
CCCATCCCTA CTGTGGTGCT ACCTGTACCG CCAACCCCAA CTGCCGCATC CCCGTCTCTC 
ACCACCAACA AAACTGGAGA TCACCAGACC ACCGTTTTGG CTCCAGCAGG GACGCCAACG 
CCTGATCCCA CATCCACCCA GTCCTCCTCA GAGCCCGAGA CGGAGACCCC GTATACCCCA 
GCTGATGCCA CCACTGCTGA CGTCAACAAG CCAGAAGATG ACACAGCGAT CATGGCAGTG 
ATGGTTGCCT TATCCTCTCT GCTGGTCATT GTCTTCATCA TCATCATCCT TTACATGCTC 
AGGTTTAAGA AGTACAAGCA GGCAGGGAGC CACTCCAACT CCTTCCGCCT GACCAACGGC 
AGAGCTGACG ACACAGAACT CCAGAGTGTA CCGTTATTGG CTCGCTCTCC AAGCACCAAC 
AGGAAGTACC CGCCTCTTCC TGTCGACAAG CTGGAGGAGG AAATGAACCG TCGAATGGCT 
GACGACAATA AACTCTTCCG GGAAGAATTC AATGCGCTGC CGGTGTGTCC CATTCAAGCC 
TCATGTGATG CCGCCTCCAA GGAGGAGAAT AAGGAGAAGA ACAGATATGT CAACATCTTG 
CCCTATGATC ATTCTAGAGT TCATTTGACC TCTCTGGAGG GTGTTCCTGA CTCAGACTAT 
ATCAACGCTT CCTACATAAA TGGATACCAG GAGAAAAACA AATTCATTGC TGCACAAGGA 
CCAAAAGAAG AGACAGTCAA TGACTACTGG AGGATGATCT GGGAGCAAAA CACTGCCACC 
ATCGTCATGG TCACCAATCT GAAGGAGCGG AAAGAGTGTA AATGTGCTCA ATACTGGCCT 
GACCAGGGAT GCTGGACTTA CGGAAACATC CGCGTGTCGG TAGAGGACAC GATGATCTTG 
GTTGACTACA CCATCCGGAA GTTCTGCATT CAGCAGGTGG GGGATGTTTC CGGTAAGAAG 
CCTCAGAGGC TGGTCACACA GTTTCATTTC ACCAGCTGGC CTGATTTTGG GGTTCCTTTC 
ACTCCGATTG GCATGCTGAA GTTCCTAAAG AAGGTCAAGA ACTGCAACCC ACAGTATGCT 
GGACCCATAA TGGTTCACTG CAGTGCGGGT GTTGGCAGGA CGGGCACCTT TATCGTGATA 
GACGCAATGC TGGACATGAT GGGTGCAGAG AGAAAGGTGG ACGTTTTCGG GTTTGTGACG 
CGGATCAGAG CCCAGCGCTG TCAGATGGTT CAGACCGATA TGCAGTATGT GTTTATTTTC 
CAAGCTTTGC TGGAGCACTA CCTGTACGGG GACACGGAGC TGGAAGTGAC GTCTCTGGAG 
ACTCACATGA ATAAACTCTA CGCTCCTTTA CCTGGAGCTG GCTGTGGAGG CCTGGAGGCA 
GAGTTTAAGA AACTTACCTC CATTAAAATC CAGAATGACA AGATGAGAAC AGGAAACCTG 
CCTGCCAACA TGAAGAAGAA TCGTGTTTTG CAAATCATTC CATATGAGTT TAACAGAGTG 
ATCATCCCTG TAAAACGTGG AGAGGAGAAC ACGGATTACA TCAACGCCTC TTTTATAGAT 
GGTTACAGGC AGAAAGACTC ATACATGGCG TGTCAGGGGC CGCTCCAGCA CACCATCGAG 
GACTACTGGA GGATGATATG GGAGTGGAGG AGCTGCTCTA TCGTTATGCT GACCGAGCTG 
GAGGAGAGAG GACAGGAGAA GTGTGCTCAG TATTGGCCTA CAGATGGTGT GATGGTTTGT 
GGAGACATGT CCATCGAGCT GAAGAGGGAG GAGGAAAGTG AGAGCTACAC TGTTAGGGAC 
CTTCTAGTCA CCAATAACAG AGAAAATAAG TCTCGCGCTG TGAGGCAGTT TCATTTCCAT 
GGCTGGCCTG AGGTGGGCAT CCCGTCTGAT GGGAAGGGCA TGATCAACAT CATCGCTGCA 
GTTCAGAAGC AGCAGCAACA GTCTGGAAAC CACCCAATCA CTGTGCACTG CAGTGCTGGT 
GCAGGACGCA CTGGGACATT CTGTGCTCTC AGCACAGTCC TGGAGCGGGT GAAGGCAGAG 
GGCATTCTGG ACGTTTTCCA GACAGTGAAG AGTCTAAGAC TACAGAGACC GCACATGGTG 
CAGACACTGG AGCAGTACGA GTTCTGCTAC AAAGTGGTCC AGGAATACAT CGATGCCTTC 
TCTGACTACG CCAACTTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324422.1
CDS	431..2914
Translation	MQVSFLQGSMGVCPLLLHVAFVALSTAQTLPHLSGAVQTTKPTPHPPTTPLIIVTNGSPTAAPTSTPSPQLIATKAFNDSEGTAAPEATTAPPVVPPPAVPIPTVVLPVPPTPTAASPSLTTNKTGDHQTTVLAPAGTPTPDPTSTQSSSEPETETPYTPADATTADVNKPEDDTAIMAVMVALSSLLVIVFIIIILYMLRFKKYKQAGSHSNSFRLTNGRADDTELQSVPLLARSPSTNRKYPPLPVDKLEEEMNRRMADDNKLFREEFNALPVCPIQASCDAASKEENKEKNRYVNILPYDHSRVHLTSLEGVPDSDYINASYINGYQEKNKFIAAQGPKEETVNDYWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDTMILVDYTIRKFCIQQVGDVSGKKPQRLVTQFHFTSWPDFGVPFTPIGMLKFLKKVKNCNPQYAGPIMVHCSAGVGRTGTFIVIDAMLDMMGAERKVDVFGFVTRIRAQRCQMVQTDMQYVFIFQALLEHYLYGDTELEVTSLETHMNKLYAPLPGAGCGGLEAEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYINASFIDGYRQKDSYMACQGPLQHTIEDYWRMIWEWRSCSIVMLTELEERGQEKCAQYWPTDGVMVCGDMSIELKREEESESYTVRDLLVTNNRENKSRAVRQFHFHGWPEVGIPSDGKGMINIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

Target ORF information:

RefSeq Version	XM_021468747.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, A (ptpra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468747.1

ORF Insert Sequence:

ATGCAGGTTT CGTTTCTCCA GGGCAGTATG GGTGTGTGTC CCCTGCTCCT GCATGTGGCC 
TTTGTGGCCC TCAGCACGGC CCAGACTCTC CCTCACCTTT CAGGTGCGGT CCAGACCACC 
AAACCTACAC CCCATCCTCC CACGACACCC CTAATCATAG TCACCAATGG ATCCCCAACA 
GCCGCTCCAA CATCCACACC GTCCCCACAA CTTATTGCAA CAAAAGCCTT CAATGACTCT 
GAGGGTACTG CGGCCCCAGA GGCTACCACT GCTCCTCCAG TCGTCCCTCC TCCAGCTGTG 
CCCATCCCTA CTGTGGTGCT ACCTGTACCG CCAACCCCAA CTGCCGCATC CCCGTCTCTC 
ACCACCAACA AAACTGGAGA TCACCAGACC ACCGTTTTGG CTCCAGCAGG GACGCCAACG 
CCTGATCCCA CATCCACCCA GTCCTCCTCA GAGCCCGAGA CGGAGACCCC GTATACCCCA 
GCTGATGCCA CCACTGCTGA CGTCAACAAG CCAGAAGATG ACACAGCGAT CATGGCAGTG 
ATGGTTGCCT TATCCTCTCT GCTGGTCATT GTCTTCATCA TCATCATCCT TTACATGCTC 
AGGTTTAAGA AGTACAAGCA GGCAGGGAGC CACTCCAACT CCTTCCGCCT GACCAACGGC 
AGAGCTGACG ACACAGAACT CCAGAGTGTA CCGTTATTGG CTCGCTCTCC AAGCACCAAC 
AGGAAGTACC CGCCTCTTCC TGTCGACAAG CTGGAGGAGG AAATGAACCG TCGAATGGCT 
GACGACAATA AACTCTTCCG GGAAGAATTC AATGCGCTGC CGGTGTGTCC CATTCAAGCC 
TCATGTGATG CCGCCTCCAA GGAGGAGAAT AAGGAGAAGA ACAGATATGT CAACATCTTG 
CCCTATGATC ATTCTAGAGT TCATTTGACC TCTCTGGAGG GTGTTCCTGA CTCAGACTAT 
ATCAACGCTT CCTACATAAA TGGATACCAG GAGAAAAACA AATTCATTGC TGCACAAGGA 
CCAAAAGAAG AGACAGTCAA TGACTACTGG AGGATGATCT GGGAGCAAAA CACTGCCACC 
ATCGTCATGG TCACCAATCT GAAGGAGCGG AAAGAGTGTA AATGTGCTCA ATACTGGCCT 
GACCAGGGAT GCTGGACTTA CGGAAACATC CGCGTGTCGG TAGAGGACAC GATGATCTTG 
GTTGACTACA CCATCCGGAA GTTCTGCATT CAGCAGGTGG GGGATGTTTC CGGTAAGAAG 
CCTCAGAGGC TGGTCACACA GTTTCATTTC ACCAGCTGGC CTGATTTTGG GGTTCCTTTC 
ACTCCGATTG GCATGCTGAA GTTCCTAAAG AAGGTCAAGA ACTGCAACCC ACAGTATGCT 
GGACCCATAA TGGTTCACTG CAGTGCGGGT GTTGGCAGGA CGGGCACCTT TATCGTGATA 
GACGCAATGC TGGACATGAT GGGTGCAGAG AGAAAGGTGG ACGTTTTCGG GTTTGTGACG 
CGGATCAGAG CCCAGCGCTG TCAGATGGTT CAGACCGATA TGCAGTATGT GTTTATTTTC 
CAAGCTTTGC TGGAGCACTA CCTGTACGGG GACACGGAGC TGGAAGTGAC GTCTCTGGAG 
ACTCACATGA ATAAACTCTA CGCTCCTTTA CCTGGAGCTG GCTGTGGAGG CCTGGAGGCA 
GAGTTTAAGA AACTTACCTC CATTAAAATC CAGAATGACA AGATGAGAAC AGGAAACCTG 
CCTGCCAACA TGAAGAAGAA TCGTGTTTTG CAAATCATTC CATATGAGTT TAACAGAGTG 
ATCATCCCTG TAAAACGTGG AGAGGAGAAC ACGGATTACA TCAACGCCTC TTTTATAGAT 
GGTTACAGGC AGAAAGACTC ATACATGGCG TGTCAGGGGC CGCTCCAGCA CACCATCGAG 
GACTACTGGA GGATGATATG GGAGTGGAGG AGCTGCTCTA TCGTTATGCT GACCGAGCTG 
GAGGAGAGAG GACAGGAGAA GTGTGCTCAG TATTGGCCTA CAGATGGTGT GATGGTTTGT 
GGAGACATGT CCATCGAGCT GAAGAGGGAG GAGGAAAGTG AGAGCTACAC TGTTAGGGAC 
CTTCTAGTCA CCAATAACAG AGAAAATAAG TCTCGCGCTG TGAGGCAGTT TCATTTCCAT 
GGCTGGCCTG AGGTGGGCAT CCCGTCTGAT GGGAAGGGCA TGATCAACAT CATCGCTGCA 
GTTCAGAAGC AGCAGCAACA GTCTGGAAAC CACCCAATCA CTGTGCACTG CAGTGCTGGT 
GCAGGACGCA CTGGGACATT CTGTGCTCTC AGCACAGTCC TGGAGCGGGT GAAGGCAGAG 
GGCATTCTGG ACGTTTTCCA GACAGTGAAG AGTCTAAGAC TACAGAGACC GCACATGGTG 
CAGACACTGG AGCAGTACGA GTTCTGCTAC AAAGTGGTCC AGGAATACAT CGATGCCTTC 
TCTGACTACG CCAACTTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Scientific reports7(1)8460(2017 Aug)
Wu W,Hale AJ,Lemeer S,den Hertog J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebrafish as a model to study PTPs during development.
Methods (San Diego, Calif.)65(2)247-53(2014 Jan)
Paardekooper Overman J,den Hertog J

Identification and expression of the family of classical protein-tyrosine phosphatases in zebrafish.
PloS one5(9)e12573(2010 Sep)
van Eekelen M,Overvoorde J,van Rooijen C,den Hertog J

RPTPalpha and PTPepsilon signaling via Fyn/Yes and RhoA is essential for zebrafish convergence and extension cell movements during gastrulation.
Developmental biology340(2)626-39(2010 Apr)
van Eekelen M,Runtuwene V,Overvoorde J,den Hertog J