efnb2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	efnb2b
Entrez Gene ID	114402
Full Name	ephrin-B2b
Synonyms	hm:zeh0344,wu:fc10d03,zeh0344
General protein information	Preferred Names ephrin-B2b Names ephrin-B2b fc10d03
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131808.1	NP_571883.1	ephrin-B2b precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Efnb2	NP_001100798.1
Bos taurus (cattle)	EFNB2	XP_002692032.2
Danio rerio (zebrafish)	efnb2a	NP_571098.1
Danio rerio (zebrafish)	LOC100333671	XP_002660391.1
Homo sapiens (human)	EFNB2	NP_004084.1
Danio rerio (zebrafish)	efnb2b	NP_571883.1
Xenopus tropicalis (tropical clawed frog)	efnb2	NP_001093709.1
	EFNB2	XP_001100069.1
Pan troglodytes (chimpanzee)	EFNB2	XP_001134800.1
Canis lupus familiaris (dog)	EFNB2	NP_001107768.1
Mus musculus (house mouse)	Efnb2	NP_034241.2
Gallus gallus (chicken)	EFNB2	NP_990155.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic regulation of left-right asymmetry by DNA methylation.
Wang L, Liu Z, Lin H, Ma D, Tao Q, Liu F
The EMBO journal36(20)2987-2997(2017 10)

Fscn1 is required for the trafficking of TGF-? family type I receptors during endoderm formation.
Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q
Nature communications712603(2016 Aug)

Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
Zhang J, Jiang Z, Liu X, Meng A
Development (Cambridge, England)143(14)2603-15(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eph receptor gene ephb4b is highly expressed in dorsal forerunner cells (DFCs) whereas ephrin ligand genes, including efnb2b, are expressed in cells next to the DFC cluster during zebrafish gastrulation.
Title: Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.

The following efnb2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efnb2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03787	NM_131808.1 Latest version!	Danio rerio ephrin-B2b (efnb2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efnb2b ( NM_131808.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571883.1 Danio rerio ephrin-B2b (efnb2b), mRNA.

Price & Availability↑

CloneID	ODa03787
Clone ID Related Accession (Same CDS sequence)	NM_131808.1
Accession Version	NM_131808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	ephrin-B2b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF375226.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF375226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476772 [ECO:0000348] ##Evidence-Data-END##

ATGGATGCGA CTGTCTGGAG TTATATATTT GGTCTCTTCG TGTTCGCTTG TCGGGTAAAA 
CTGACTCGAT CAACAGTTTT AGAATCAATA TATTGGAATA CTTCCAACAC CAAGTTTGTC 
CCAGGAAGAG GTGTAGTATT ATACCCTCAG ATCGGAGACA AAATGGACAT TGTGTGTCCA 
AGAATCAAGC CAGGCTCCAC TGAGCAGACG AACATTGAAT ACTTCCGGGT CTATCTTGTT 
CCCAAAGAAC AACTCGAGAC CTGCCATGTT ACTAAAAGCG ACATGTTACT GCTCAACTGT 
GACAAGCCGG ACCAGGACGT CAAATTCACC TTCAAGTTTC AAGAGTTCAG CCCCAACCTG 
TGGGGCCTGG AGTTCTTAAG AGGGAAGGAC TATCACATCA TCTCCACCTC TAACAGCACT 
TTTGAAGGAC TGGACAACCA CCATGGTGGC GTTTGCAGGA GCAAATCCAT GAAGCTGGTT 
TTGCGGGTTG GCCAGAGTCC TACAGATTCA TTCTCAGCGA AAAACCATCC CACAAGAAAT 
CCTCCAAAAT ACCCTGAAAA TAAGGACCAA AATACCTTCA GCAAAGAAAA TGATGTCAGT 
CAGATTGACT CCATGCAGAA CGGAGAATCC GGAGGGAAGA GTGGCGAGTC TGTGGGATCT 
GCTGGGTCTG ATGTGGCCCT GTTTGCAGGC GTCGCATCCG GGGCTGTGAT CTTCATCCTC 
ATCATCATCG CACTTGTAGC ACTTCTGCAT CGGCGTCATC AGAAGCACTC GGCGCAGTGC 
TCCGGACAGC TGCCCTTAAA CACGCTGCCC AAACGTGGCA GTGGCGCCAG CGGCGGCAGC 
AACAACAACG GCTCCGAGCC CAGCGACATC ATCTTTCCTA TCCGGACCTC AGGAAGCATG 
TACTGCCCGC ATTACGAGAA GGTTAGCGGA GACTACGGAC ACCCCGTGTA CATCGTCCAA 
GAAATGCCAC CGCAGAATCC GGCAAATATT TACTACAAGG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571883.1
CDS	1..1005
Translation	MDATVWSYIFGLFVFACRVKLTRSTVLESIYWNTSNTKFVPGRGVVLYPQIGDKMDIVCPRIKPGSTEQTNIEYFRVYLVPKEQLETCHVTKSDMLLLNCDKPDQDVKFTFKFQEFSPNLWGLEFLRGKDYHIISTSNSTFEGLDNHHGGVCRSKSMKLVLRVGQSPTDSFSAKNHPTRNPPKYPENKDQNTFSKENDVSQIDSMQNGESGGKSGESVGSAGSDVALFAGVASGAVIFILIIIALVALLHRRHQKHSAQCSGQLPLNTLPKRGSGASGGSNNNGSEPSDIIFPIRTSGSMYCPHYEKVSGDYGHPVYIVQEMPPQNPANIYYKV

Target ORF information:

RefSeq Version	NM_131808.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ephrin-B2b (efnb2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131808.1

ORF Insert Sequence:

ATGGATGCGA CTGTCTGGAG TTATATATTT GGTCTCTTCG TGTTCGCTTG TCGGGTAAAA 
CTGACTCGAT CAACAGTTTT AGAATCAATA TATTGGAATA CTTCCAACAC CAAGTTTGTC 
CCAGGAAGAG GTGTAGTATT ATACCCTCAG ATCGGAGACA AAATGGACAT TGTGTGTCCA 
AGAATCAAGC CAGGCTCCAC TGAGCAGACG AACATTGAAT ACTTCCGGGT CTATCTTGTT 
CCCAAAGAAC AACTCGAGAC CTGCCATGTT ACTAAAAGCG ACATGTTACT GCTCAACTGT 
GACAAGCCGG ACCAGGACGT CAAATTCACC TTCAAGTTTC AAGAGTTCAG CCCCAACCTG 
TGGGGCCTGG AGTTCTTAAG AGGGAAGGAC TATCACATCA TCTCCACCTC TAACAGCACT 
TTTGAAGGAC TGGACAACCA CCATGGTGGC GTTTGCAGGA GCAAATCCAT GAAGCTGGTT 
TTGCGGGTTG GCCAGAGTCC TACAGATTCA TTCTCAGCGA AAAACCATCC CACAAGAAAT 
CCTCCAAAAT ACCCTGAAAA TAAGGACCAA AATACCTTCA GCAAAGAAAA TGATGTCAGT 
CAGATTGACT CCATGCAGAA CGGAGAATCC GGAGGGAAGA GTGGCGAGTC TGTGGGATCT 
GCTGGGTCTG ATGTGGCCCT GTTTGCAGGC GTCGCATCCG GGGCTGTGAT CTTCATCCTC 
ATCATCATCG CACTTGTAGC ACTTCTGCAT CGGCGTCATC AGAAGCACTC GGCGCAGTGC 
TCCGGACAGC TGCCCTTAAA CACGCTGCCC AAACGTGGCA GTGGCGCCAG CGGCGGCAGC 
AACAACAACG GCTCCGAGCC CAGCGACATC ATCTTTCCTA TCCGGACCTC AGGAAGCATG 
TACTGCCCGC ATTACGAGAA GGTTAGCGGA GACTACGGAC ACCCCGTGTA CATCGTCCAA 
GAAATGCCAC CGCAGAATCC GGCAAATATT TACTACAAGG TTTGA