SLF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLF1
Entrez Gene ID	101882450
Full Name	SMC5-SMC6 complex localization factor 1
Synonyms	ankrd32,si:ch73-280o22.1
General protein information	Preferred Names SMC5-SMC6 complex localization factor 1 Names SMC5-SMC6 complex localization factor protein 1 ankyrin repeat domain 32
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165540.4	XP_005165597.2	SMC5-SMC6 complex localization factor protein 1
XM_005165540.3	XP_005165597.2	SMC5-SMC6 complex localization factor protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ANKRD32	NP_001095528.2
Rattus norvegicus (Norway rat)	Ankrd32	NP_001099870.1
Homo sapiens (human)	ANKRD32	NP_115666.2
Pan troglodytes (chimpanzee)	ANKRD32	XP_517654.3
Mus musculus (house mouse)	Ankrd32	NP_598832.3
Xenopus tropicalis (tropical clawed frog)	ankrd32	XP_002933469.2
Canis lupus familiaris (dog)	ANKRD32	XP_003434463.1
Gallus gallus (chicken)	ANKRD32	XP_004949455.1
Danio rerio (zebrafish)	LOC101882450	XP_005165597.1
	ANKRD32	XP_001090273.2

Gene Ontology

The following SLF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa50803	XM_005165540.4 Latest version!	Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa50803	XM_005165540.3	Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLF1 ( XM_005165540.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165597.2 Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.

Price & Availability↑

CloneID	ODa50803
Clone ID Related Accession (Same CDS sequence)	XM_005165540.4 , XM_005165540.3
Accession Version	XM_005165540.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SMC5-SMC6 complex localization factor protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165540.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA ATACATATGT CTTTCAAACG TCTGGCTTCA GGAATAATTC TCAAAAAAGA 
CTTTTACTTC AGGGCATCAA ACATCTAGGG GGCGTATACA TGGGGGGCTC TATTTATAAG 
GAAGCAATCA CTCACCTCAT CGTTGAAAAA GCTTTACCCA GTGAAAAGTT TCTTGCAGCC 
TCTGCGGGAG GGAAGTGGAT AGTGACTCCT GAGTTTGTTC TGGACAGTGT GAAGCACAAG 
AGATGGCTAC AAGAGACTTC ATATGAGATA AACCTAACAG CTCAGACCCC AGAGACGCCT 
AATCCATTGA GAACTTGGAG AGAGAGAGTA GCGAATGGCA CAGTGTCGGG AGCCTTTCAG 
GACTGGGTAG TGCTCATTGA CATTGATGAC CCAACGAGGA GGAGTGTGTT TGTCAGGATA 
TTGAAAGCAG GCAAAGCTGT TGTCTGCACA GACTGGCCAA CATCATGTGC TGTAACTCAT 
GTGTTAGCCA AAAATCCATC CAAAGAGGGA GCTGTGCAGG GTGCAGTCCA TCGCTATAGC 
GTCAGCTATG TAGCCAAACA TCTTTTTGGG AATATTTGCT CAGAATTAAA CTGGTCCTGT 
GATTTAGAGT CTTTCGATGA AGCAGAGAGC TGTATAACAG CCACCCAGAC TGCTGCAGTC 
ACTGACGAAT CTGTAGATGT CACTTCTGAC TCTTCACATA AAGAACTGGA GGACATGCTC 
AAAGAATACA TCATAATAAT GGAGCAAAGA AAGAAGAAGC TTTTGATGGT GCCACAGTTT 
CACAGCTACT ACACACCCGT CCTGCCAGAT CAGATTCCAG CAGTGGATTT TAGCAATGTG 
CGGAGTTTGG TGGAGTGTTC CTTCTTCCCT CAAGCTGTGG AGGAGATTTT AGGGTCCCTG 
CATCCTGGAG TGATGCCCCC AGCCACACTG CTCCAAGAGC TAATGCGCCA TGCACTGCAT 
GGTGATGCAA AGCCATTGTA CCTCAGCACA TTTATCACAG TTCTCCATAG TATCCTGCGT 
AACAATCCAA CCTGGGGCTG TCCTGGAAAC ACTAAGTACT TCCTTAAGGT CCTCCAGTGT 
CCAGAATGTA AAAACGGTCT CTGGTCACTT CTTGAGACGT CACTTCAAGT GTGTTTGGCA 
AGCGAACCCA CCTGTCACTC ACTGCCAAGC CCACCATCCA CTGAGATGTT TCGCCTCCAC 
TGTAACCTGC AGGAGTTCTT CCTGAGACTG TTTCAGCTGG AGCTTCATGC TGCTAGCTCA 
GGAAGATCAC CTGGAAGCAG TCGAGCATCT GTTCTCTATA GTACTTTCTG GAGCACGTGG 
GAAAAGTCAA CTCTAAACTC CAGAGCAGTT CGGCAGCTCA GCAGACTTTT GATCAAGACT 
TTAATTTGGG CTTACAACTG CAATCAGGAG CGCAGGCTGG GAATGTTGGT CACTCTGCAG 
GAGACACTGT GTGTAGTGGT AGAGTTCTGG GCTCAGGAAC ACAGTCCACT CAACAGCAGT 
CTGGTGCAAA AAGGCTTTAA AGATCTGGCT GAACACATCG CTCTACTGTG CCAAGACCTT 
GACTTTGACA TGTTGAAGGA GGTGGTTCCA GCCCTTCGCT CCGCAAGACT CCGGATGTTC 
ACTGCCGATG CAATTTACAG AAACATCTGT TGCAGCAGGG GCCTGTTCTT CAGCTCTGAA 
CCACTCTGCC TGCTCAAAAT TGTCTCAACC TACCTCAAAG CTCTGGGGAA CTTGTGTGAT 
CGAGAACCAG TCCAAACCCT GAAGGGACAG ACTGACAGAA TCGAGATGAA TCAACCATCT 
ACCAGCAGTG CCACCCGAGT GCCAGTCTCA GAGACTGTGA TGGACTCAGC AGGCGGCTTA 
GGTTCGGGAA GAGAGACTGT CCCGAGAGGT TTTCACAGAG TCAATGCTGC AGGCGAGACA 
CTCCTTCATC GAGCCTGCAA GAGGAACCAG GTGGAGACAC TGCTTCGTAT CCTCACCATG 
CCTGAAACGG ACGTCAACAT TAAAGATCAC GCTGGTTGGA CTCCCCTTCA TGAAGCCTGT 
AACCATGGCA GTGCAGAGTG TGTGCAGGCC CTTCTGCAGC ACTGCCCAAA CCTGCAGCTG 
GGCACTCTGG TCCAGGGAGT CAGTCCACTA CATGACGCCC TGCTGAACAA CCACATCCAT 
ATCGCCAAGA TGCTGCTGAA GCACGGCGGC TCACCTTTAC TGAAGTTACG TAACAATGAC 
GGTCTCACCC CTCTGGACCT GGTCCCTGTA GGGGCTCTCC GTGAGGAGCT CCTCCACTGT 
GCAGAAGAAC GGGACAAATT TCTGGAGTCT TCCTGCCTGG AGGTGCGGGA TCTCCCTTTG 
ATCGAGTGCT GCTCATGGGT GCTCGCCTGC TTGTTCCTCA CCTACACTCA TGAGCAGTAC 
ATATCTACTT ACAAAACCCC CCTCACGCCT CTGGACTTGA GTCCGGATGT GTGCCGCTTG 
CTTATCCATC TGCGTCCCGG TGCACTGGCC TCCACCTGGG GGGATTCACG TGCGGTGACC 
CTGGCCGAGG ACTTGAGAAC TATGTTAAGT TTGGAGCAAT ACATGTCCAA GGTGAGCCCT 
GCTCTGAGAC GCTGTCAGGG GGATCAGACC AGCCTGTTTG TGCAGCTGCT GGAAGACCTG 
CAGGCAGAGG GAGCGGCACT GATCGCCGGA CAGTCTGATT CTGATTCCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165597.2
CDS	273..2966
Translation	MVDNTYVFQTSGFRNNSQKRLLLQGIKHLGGVYMGGSIYKEAITHLIVEKALPSEKFLAASAGGKWIVTPEFVLDSVKHKRWLQETSYEINLTAQTPETPNPLRTWRERVANGTVSGAFQDWVVLIDIDDPTRRSVFVRILKAGKAVVCTDWPTSCAVTHVLAKNPSKEGAVQGAVHRYSVSYVAKHLFGNICSELNWSCDLESFDEAESCITATQTAAVTDESVDVTSDSSHKELEDMLKEYIIIMEQRKKKLLMVPQFHSYYTPVLPDQIPAVDFSNVRSLVECSFFPQAVEEILGSLHPGVMPPATLLQELMRHALHGDAKPLYLSTFITVLHSILRNNPTWGCPGNTKYFLKVLQCPECKNGLWSLLETSLQVCLASEPTCHSLPSPPSTEMFRLHCNLQEFFLRLFQLELHAASSGRSPGSSRASVLYSTFWSTWEKSTLNSRAVRQLSRLLIKTLIWAYNCNQERRLGMLVTLQETLCVVVEFWAQEHSPLNSSLVQKGFKDLAEHIALLCQDLDFDMLKEVVPALRSARLRMFTADAIYRNICCSRGLFFSSEPLCLLKIVSTYLKALGNLCDREPVQTLKGQTDRIEMNQPSTSSATRVPVSETVMDSAGGLGSGRETVPRGFHRVNAAGETLLHRACKRNQVETLLRILTMPETDVNIKDHAGWTPLHEACNHGSAECVQALLQHCPNLQLGTLVQGVSPLHDALLNNHIHIAKMLLKHGGSPLLKLRNNDGLTPLDLVPVGALREELLHCAEERDKFLESSCLEVRDLPLIECCSWVLACLFLTYTHEQYISTYKTPLTPLDLSPDVCRLLIHLRPGALASTWGDSRAVTLAEDLRTMLSLEQYMSKVSPALRRCQGDQTSLFVQLLEDLQAEGAALIAGQSDSDSF

Target ORF information:

RefSeq Version	XM_005165540.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165540.4

ORF Insert Sequence:

ATGGTGGACA ATACATATGT CTTTCAAACG TCTGGCTTCA GGAATAATTC TCAAAAAAGA 
CTTTTACTTC AGGGCATCAA ACATCTAGGG GGCGTATACA TGGGGGGCTC TATTTATAAG 
GAAGCAATCA CTCACCTCAT CGTTGAAAAA GCTTTACCCA GTGAAAAGTT TCTTGCAGCC 
TCTGCGGGAG GGAAGTGGAT AGTGACTCCT GAGTTTGTTC TGGACAGTGT GAAGCACAAG 
AGATGGCTAC AAGAGACTTC ATATGAGATA AACCTAACAG CTCAGACCCC AGAGACGCCT 
AATCCATTGA GAACTTGGAG AGAGAGAGTA GCGAATGGCA CAGTGTCGGG AGCCTTTCAG 
GACTGGGTAG TGCTCATTGA CATTGATGAC CCAACGAGGA GGAGTGTGTT TGTCAGGATA 
TTGAAAGCAG GCAAAGCTGT TGTCTGCACA GACTGGCCAA CATCATGTGC TGTAACTCAT 
GTGTTAGCCA AAAATCCATC CAAAGAGGGA GCTGTGCAGG GTGCAGTCCA TCGCTATAGC 
GTCAGCTATG TAGCCAAACA TCTTTTTGGG AATATTTGCT CAGAATTAAA CTGGTCCTGT 
GATTTAGAGT CTTTCGATGA AGCAGAGAGC TGTATAACAG CCACCCAGAC TGCTGCAGTC 
ACTGACGAAT CTGTAGATGT CACTTCTGAC TCTTCACATA AAGAACTGGA GGACATGCTC 
AAAGAATACA TCATAATAAT GGAGCAAAGA AAGAAGAAGC TTTTGATGGT GCCACAGTTT 
CACAGCTACT ACACACCCGT CCTGCCAGAT CAGATTCCAG CAGTGGATTT TAGCAATGTG 
CGGAGTTTGG TGGAGTGTTC CTTCTTCCCT CAAGCTGTGG AGGAGATTTT AGGGTCCCTG 
CATCCTGGAG TGATGCCCCC AGCCACACTG CTCCAAGAGC TAATGCGCCA TGCACTGCAT 
GGTGATGCAA AGCCATTGTA CCTCAGCACA TTTATCACAG TTCTCCATAG TATCCTGCGT 
AACAATCCAA CCTGGGGCTG TCCTGGAAAC ACTAAGTACT TCCTTAAGGT CCTCCAGTGT 
CCAGAATGTA AAAACGGTCT CTGGTCACTT CTTGAGACGT CACTTCAAGT GTGTTTGGCA 
AGCGAACCCA CCTGTCACTC ACTGCCAAGC CCACCATCCA CTGAGATGTT TCGCCTCCAC 
TGTAACCTGC AGGAGTTCTT CCTGAGACTG TTTCAGCTGG AGCTTCATGC TGCTAGCTCA 
GGAAGATCAC CTGGAAGCAG TCGAGCATCT GTTCTCTATA GTACTTTCTG GAGCACGTGG 
GAAAAGTCAA CTCTAAACTC CAGAGCAGTT CGGCAGCTCA GCAGACTTTT GATCAAGACT 
TTAATTTGGG CTTACAACTG CAATCAGGAG CGCAGGCTGG GAATGTTGGT CACTCTGCAG 
GAGACACTGT GTGTAGTGGT AGAGTTCTGG GCTCAGGAAC ACAGTCCACT CAACAGCAGT 
CTGGTGCAAA AAGGCTTTAA AGATCTGGCT GAACACATCG CTCTACTGTG CCAAGACCTT 
GACTTTGACA TGTTGAAGGA GGTGGTTCCA GCCCTTCGCT CCGCAAGACT CCGGATGTTC 
ACTGCCGATG CAATTTACAG AAACATCTGT TGCAGCAGGG GCCTGTTCTT CAGCTCTGAA 
CCACTCTGCC TGCTCAAAAT TGTCTCAACC TACCTCAAAG CTCTGGGGAA CTTGTGTGAT 
CGAGAACCAG TCCAAACCCT GAAGGGACAG ACTGACAGAA TCGAGATGAA TCAACCATCT 
ACCAGCAGTG CCACCCGAGT GCCAGTCTCA GAGACTGTGA TGGACTCAGC AGGCGGCTTA 
GGTTCGGGAA GAGAGACTGT CCCGAGAGGT TTTCACAGAG TCAATGCTGC AGGCGAGACA 
CTCCTTCATC GAGCCTGCAA GAGGAACCAG GTGGAGACAC TGCTTCGTAT CCTCACCATG 
CCTGAAACGG ACGTCAACAT TAAAGATCAC GCTGGTTGGA CTCCCCTTCA TGAAGCCTGT 
AACCATGGCA GTGCAGAGTG TGTGCAGGCC CTTCTGCAGC ACTGCCCAAA CCTGCAGCTG 
GGCACTCTGG TCCAGGGAGT CAGTCCACTA CATGACGCCC TGCTGAACAA CCACATCCAT 
ATCGCCAAGA TGCTGCTGAA GCACGGCGGC TCACCTTTAC TGAAGTTACG TAACAATGAC 
GGTCTCACCC CTCTGGACCT GGTCCCTGTA GGGGCTCTCC GTGAGGAGCT CCTCCACTGT 
GCAGAAGAAC GGGACAAATT TCTGGAGTCT TCCTGCCTGG AGGTGCGGGA TCTCCCTTTG 
ATCGAGTGCT GCTCATGGGT GCTCGCCTGC TTGTTCCTCA CCTACACTCA TGAGCAGTAC 
ATATCTACTT ACAAAACCCC CCTCACGCCT CTGGACTTGA GTCCGGATGT GTGCCGCTTG 
CTTATCCATC TGCGTCCCGG TGCACTGGCC TCCACCTGGG GGGATTCACG TGCGGTGACC 
CTGGCCGAGG ACTTGAGAAC TATGTTAAGT TTGGAGCAAT ACATGTCCAA GGTGAGCCCT 
GCTCTGAGAC GCTGTCAGGG GGATCAGACC AGCCTGTTTG TGCAGCTGCT GGAAGACCTG 
CAGGCAGAGG GAGCGGCACT GATCGCCGGA CAGTCTGATT CTGATTCCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLF1 ( XM_005165540.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165597.2 Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.

Price & Availability↑

CloneID	ODa50803
Clone ID Related Accession (Same CDS sequence)	XM_005165540.4 , XM_005165540.3
Accession Version	XM_005165540.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SMC5-SMC6 complex localization factor protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA ATACATATGT CTTTCAAACG TCTGGCTTCA GGAATAATTC TCAAAAAAGA 
CTTTTACTTC AGGGCATCAA ACATCTAGGG GGCGTATACA TGGGGGGCTC TATTTATAAG 
GAAGCAATCA CTCACCTCAT CGTTGAAAAA GCTTTACCCA GTGAAAAGTT TCTTGCAGCC 
TCTGCGGGAG GGAAGTGGAT AGTGACTCCT GAGTTTGTTC TGGACAGTGT GAAGCACAAG 
AGATGGCTAC AAGAGACTTC ATATGAGATA AACCTAACAG CTCAGACCCC AGAGACGCCT 
AATCCATTGA GAACTTGGAG AGAGAGAGTA GCGAATGGCA CAGTGTCGGG AGCCTTTCAG 
GACTGGGTAG TGCTCATTGA CATTGATGAC CCAACGAGGA GGAGTGTGTT TGTCAGGATA 
TTGAAAGCAG GCAAAGCTGT TGTCTGCACA GACTGGCCAA CATCATGTGC TGTAACTCAT 
GTGTTAGCCA AAAATCCATC CAAAGAGGGA GCTGTGCAGG GTGCAGTCCA TCGCTATAGC 
GTCAGCTATG TAGCCAAACA TCTTTTTGGG AATATTTGCT CAGAATTAAA CTGGTCCTGT 
GATTTAGAGT CTTTCGATGA AGCAGAGAGC TGTATAACAG CCACCCAGAC TGCTGCAGTC 
ACTGACGAAT CTGTAGATGT CACTTCTGAC TCTTCACATA AAGAACTGGA GGACATGCTC 
AAAGAATACA TCATAATAAT GGAGCAAAGA AAGAAGAAGC TTTTGATGGT GCCACAGTTT 
CACAGCTACT ACACACCCGT CCTGCCAGAT CAGATTCCAG CAGTGGATTT TAGCAATGTG 
CGGAGTTTGG TGGAGTGTTC CTTCTTCCCT CAAGCTGTGG AGGAGATTTT AGGGTCCCTG 
CATCCTGGAG TGATGCCCCC AGCCACACTG CTCCAAGAGC TAATGCGCCA TGCACTGCAT 
GGTGATGCAA AGCCATTGTA CCTCAGCACA TTTATCACAG TTCTCCATAG TATCCTGCGT 
AACAATCCAA CCTGGGGCTG TCCTGGAAAC ACTAAGTACT TCCTTAAGGT CCTCCAGTGT 
CCAGAATGTA AAAACGGTCT CTGGTCACTT CTTGAGACGT CACTTCAAGT GTGTTTGGCA 
AGCGAACCCA CCTGTCACTC ACTGCCAAGC CCACCATCCA CTGAGATGTT TCGCCTCCAC 
TGTAACCTGC AGGAGTTCTT CCTGAGACTG TTTCAGCTGG AGCTTCATGC TGCTAGCTCA 
GGAAGATCAC CTGGAAGCAG TCGAGCATCT GTTCTCTATA GTACTTTCTG GAGCACGTGG 
GAAAAGTCAA CTCTAAACTC CAGAGCAGTT CGGCAGCTCA GCAGACTTTT GATCAAGACT 
TTAATTTGGG CTTACAACTG CAATCAGGAG CGCAGGCTGG GAATGTTGGT CACTCTGCAG 
GAGACACTGT GTGTAGTGGT AGAGTTCTGG GCTCAGGAAC ACAGTCCACT CAACAGCAGT 
CTGGTGCAAA AAGGCTTTAA AGATCTGGCT GAACACATCG CTCTACTGTG CCAAGACCTT 
GACTTTGACA TGTTGAAGGA GGTGGTTCCA GCCCTTCGCT CCGCAAGACT CCGGATGTTC 
ACTGCCGATG CAATTTACAG AAACATCTGT TGCAGCAGGG GCCTGTTCTT CAGCTCTGAA 
CCACTCTGCC TGCTCAAAAT TGTCTCAACC TACCTCAAAG CTCTGGGGAA CTTGTGTGAT 
CGAGAACCAG TCCAAACCCT GAAGGGACAG ACTGACAGAA TCGAGATGAA TCAACCATCT 
ACCAGCAGTG CCACCCGAGT GCCAGTCTCA GAGACTGTGA TGGACTCAGC AGGCGGCTTA 
GGTTCGGGAA GAGAGACTGT CCCGAGAGGT TTTCACAGAG TCAATGCTGC AGGCGAGACA 
CTCCTTCATC GAGCCTGCAA GAGGAACCAG GTGGAGACAC TGCTTCGTAT CCTCACCATG 
CCTGAAACGG ACGTCAACAT TAAAGATCAC GCTGGTTGGA CTCCCCTTCA TGAAGCCTGT 
AACCATGGCA GTGCAGAGTG TGTGCAGGCC CTTCTGCAGC ACTGCCCAAA CCTGCAGCTG 
GGCACTCTGG TCCAGGGAGT CAGTCCACTA CATGACGCCC TGCTGAACAA CCACATCCAT 
ATCGCCAAGA TGCTGCTGAA GCACGGCGGC TCACCTTTAC TGAAGTTACG TAACAATGAC 
GGTCTCACCC CTCTGGACCT GGTCCCTGTA GGGGCTCTCC GTGAGGAGCT CCTCCACTGT 
GCAGAAGAAC GGGACAAATT TCTGGAGTCT TCCTGCCTGG AGGTGCGGGA TCTCCCTTTG 
ATCGAGTGCT GCTCATGGGT GCTCGCCTGC TTGTTCCTCA CCTACACTCA TGAGCAGTAC 
ATATCTACTT ACAAAACCCC CCTCACGCCT CTGGACTTGA GTCCGGATGT GTGCCGCTTG 
CTTATCCATC TGCGTCCCGG TGCACTGGCC TCCACCTGGG GGGATTCACG TGCGGTGACC 
CTGGCCGAGG ACTTGAGAAC TATGTTAAGT TTGGAGCAAT ACATGTCCAA GGTGAGCCCT 
GCTCTGAGAC GCTGTCAGGG GGATCAGACC AGCCTGTTTG TGCAGCTGCT GGAAGACCTG 
CAGGCAGAGG GAGCGGCACT GATCGCCGGA CAGTCTGATT CTGATTCCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165597.2
CDS	263..2956
Translation	MVDNTYVFQTSGFRNNSQKRLLLQGIKHLGGVYMGGSIYKEAITHLIVEKALPSEKFLAASAGGKWIVTPEFVLDSVKHKRWLQETSYEINLTAQTPETPNPLRTWRERVANGTVSGAFQDWVVLIDIDDPTRRSVFVRILKAGKAVVCTDWPTSCAVTHVLAKNPSKEGAVQGAVHRYSVSYVAKHLFGNICSELNWSCDLESFDEAESCITATQTAAVTDESVDVTSDSSHKELEDMLKEYIIIMEQRKKKLLMVPQFHSYYTPVLPDQIPAVDFSNVRSLVECSFFPQAVEEILGSLHPGVMPPATLLQELMRHALHGDAKPLYLSTFITVLHSILRNNPTWGCPGNTKYFLKVLQCPECKNGLWSLLETSLQVCLASEPTCHSLPSPPSTEMFRLHCNLQEFFLRLFQLELHAASSGRSPGSSRASVLYSTFWSTWEKSTLNSRAVRQLSRLLIKTLIWAYNCNQERRLGMLVTLQETLCVVVEFWAQEHSPLNSSLVQKGFKDLAEHIALLCQDLDFDMLKEVVPALRSARLRMFTADAIYRNICCSRGLFFSSEPLCLLKIVSTYLKALGNLCDREPVQTLKGQTDRIEMNQPSTSSATRVPVSETVMDSAGGLGSGRETVPRGFHRVNAAGETLLHRACKRNQVETLLRILTMPETDVNIKDHAGWTPLHEACNHGSAECVQALLQHCPNLQLGTLVQGVSPLHDALLNNHIHIAKMLLKHGGSPLLKLRNNDGLTPLDLVPVGALREELLHCAEERDKFLESSCLEVRDLPLIECCSWVLACLFLTYTHEQYISTYKTPLTPLDLSPDVCRLLIHLRPGALASTWGDSRAVTLAEDLRTMLSLEQYMSKVSPALRRCQGDQTSLFVQLLEDLQAEGAALIAGQSDSDSF

Target ORF information:

RefSeq Version	XM_005165540.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMC5-SMC6 complex localization factor 1 (slf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165540.3

ORF Insert Sequence:

ATGGTGGACA ATACATATGT CTTTCAAACG TCTGGCTTCA GGAATAATTC TCAAAAAAGA 
CTTTTACTTC AGGGCATCAA ACATCTAGGG GGCGTATACA TGGGGGGCTC TATTTATAAG 
GAAGCAATCA CTCACCTCAT CGTTGAAAAA GCTTTACCCA GTGAAAAGTT TCTTGCAGCC 
TCTGCGGGAG GGAAGTGGAT AGTGACTCCT GAGTTTGTTC TGGACAGTGT GAAGCACAAG 
AGATGGCTAC AAGAGACTTC ATATGAGATA AACCTAACAG CTCAGACCCC AGAGACGCCT 
AATCCATTGA GAACTTGGAG AGAGAGAGTA GCGAATGGCA CAGTGTCGGG AGCCTTTCAG 
GACTGGGTAG TGCTCATTGA CATTGATGAC CCAACGAGGA GGAGTGTGTT TGTCAGGATA 
TTGAAAGCAG GCAAAGCTGT TGTCTGCACA GACTGGCCAA CATCATGTGC TGTAACTCAT 
GTGTTAGCCA AAAATCCATC CAAAGAGGGA GCTGTGCAGG GTGCAGTCCA TCGCTATAGC 
GTCAGCTATG TAGCCAAACA TCTTTTTGGG AATATTTGCT CAGAATTAAA CTGGTCCTGT 
GATTTAGAGT CTTTCGATGA AGCAGAGAGC TGTATAACAG CCACCCAGAC TGCTGCAGTC 
ACTGACGAAT CTGTAGATGT CACTTCTGAC TCTTCACATA AAGAACTGGA GGACATGCTC 
AAAGAATACA TCATAATAAT GGAGCAAAGA AAGAAGAAGC TTTTGATGGT GCCACAGTTT 
CACAGCTACT ACACACCCGT CCTGCCAGAT CAGATTCCAG CAGTGGATTT TAGCAATGTG 
CGGAGTTTGG TGGAGTGTTC CTTCTTCCCT CAAGCTGTGG AGGAGATTTT AGGGTCCCTG 
CATCCTGGAG TGATGCCCCC AGCCACACTG CTCCAAGAGC TAATGCGCCA TGCACTGCAT 
GGTGATGCAA AGCCATTGTA CCTCAGCACA TTTATCACAG TTCTCCATAG TATCCTGCGT 
AACAATCCAA CCTGGGGCTG TCCTGGAAAC ACTAAGTACT TCCTTAAGGT CCTCCAGTGT 
CCAGAATGTA AAAACGGTCT CTGGTCACTT CTTGAGACGT CACTTCAAGT GTGTTTGGCA 
AGCGAACCCA CCTGTCACTC ACTGCCAAGC CCACCATCCA CTGAGATGTT TCGCCTCCAC 
TGTAACCTGC AGGAGTTCTT CCTGAGACTG TTTCAGCTGG AGCTTCATGC TGCTAGCTCA 
GGAAGATCAC CTGGAAGCAG TCGAGCATCT GTTCTCTATA GTACTTTCTG GAGCACGTGG 
GAAAAGTCAA CTCTAAACTC CAGAGCAGTT CGGCAGCTCA GCAGACTTTT GATCAAGACT 
TTAATTTGGG CTTACAACTG CAATCAGGAG CGCAGGCTGG GAATGTTGGT CACTCTGCAG 
GAGACACTGT GTGTAGTGGT AGAGTTCTGG GCTCAGGAAC ACAGTCCACT CAACAGCAGT 
CTGGTGCAAA AAGGCTTTAA AGATCTGGCT GAACACATCG CTCTACTGTG CCAAGACCTT 
GACTTTGACA TGTTGAAGGA GGTGGTTCCA GCCCTTCGCT CCGCAAGACT CCGGATGTTC 
ACTGCCGATG CAATTTACAG AAACATCTGT TGCAGCAGGG GCCTGTTCTT CAGCTCTGAA 
CCACTCTGCC TGCTCAAAAT TGTCTCAACC TACCTCAAAG CTCTGGGGAA CTTGTGTGAT 
CGAGAACCAG TCCAAACCCT GAAGGGACAG ACTGACAGAA TCGAGATGAA TCAACCATCT 
ACCAGCAGTG CCACCCGAGT GCCAGTCTCA GAGACTGTGA TGGACTCAGC AGGCGGCTTA 
GGTTCGGGAA GAGAGACTGT CCCGAGAGGT TTTCACAGAG TCAATGCTGC AGGCGAGACA 
CTCCTTCATC GAGCCTGCAA GAGGAACCAG GTGGAGACAC TGCTTCGTAT CCTCACCATG 
CCTGAAACGG ACGTCAACAT TAAAGATCAC GCTGGTTGGA CTCCCCTTCA TGAAGCCTGT 
AACCATGGCA GTGCAGAGTG TGTGCAGGCC CTTCTGCAGC ACTGCCCAAA CCTGCAGCTG 
GGCACTCTGG TCCAGGGAGT CAGTCCACTA CATGACGCCC TGCTGAACAA CCACATCCAT 
ATCGCCAAGA TGCTGCTGAA GCACGGCGGC TCACCTTTAC TGAAGTTACG TAACAATGAC 
GGTCTCACCC CTCTGGACCT GGTCCCTGTA GGGGCTCTCC GTGAGGAGCT CCTCCACTGT 
GCAGAAGAAC GGGACAAATT TCTGGAGTCT TCCTGCCTGG AGGTGCGGGA TCTCCCTTTG 
ATCGAGTGCT GCTCATGGGT GCTCGCCTGC TTGTTCCTCA CCTACACTCA TGAGCAGTAC 
ATATCTACTT ACAAAACCCC CCTCACGCCT CTGGACTTGA GTCCGGATGT GTGCCGCTTG 
CTTATCCATC TGCGTCCCGG TGCACTGGCC TCCACCTGGG GGGATTCACG TGCGGTGACC 
CTGGCCGAGG ACTTGAGAAC TATGTTAAGT TTGGAGCAAT ACATGTCCAA GGTGAGCCCT 
GCTCTGAGAC GCTGTCAGGG GGATCAGACC AGCCTGTTTG TGCAGCTGCT GGAAGACCTG 
CAGGCAGAGG GAGCGGCACT GATCGCCGGA CAGTCTGATT CTGATTCCTT CTAG