kif1aa gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021477120.1	XP_021332795.1	kinesin-like protein KIF1A isoform X1
XM_021477127.1	XP_021332802.1	kinesin-like protein KIF1A isoform X7
XM_021477128.1	XP_021332803.1	kinesin-like protein KIF1A isoform X8
XM_021477129.1	XP_021332804.1	kinesin-like protein KIF1A isoform X10
XM_005165954.4	XP_005166011.1	kinesin-like protein KIF1A isoform X15
XM_005165952.3	XP_005166009.1	kinesin-like protein KIF1A isoform X8
XM_005165954.3	XP_005166011.1	kinesin-like protein KIF1A isoform X10
XM_005165946.3	XP_005166003.1	kinesin-like protein KIF1A isoform X2
XM_005165949.3	XP_005166006.1	kinesin-like protein KIF1A isoform X5
XM_021477122.1	XP_021332797.1	kinesin-like protein KIF1A isoform X3
XM_021477125.1	XP_021332800.1	kinesin-like protein KIF1A isoform X6
XM_005165945.4	XP_005166002.1	kinesin-like protein KIF1A isoform X9
XM_005165950.4	XP_005166007.1	kinesin-like protein KIF1A isoform X11
XM_021477131.1	XP_021332806.1	kinesin-like protein KIF1A isoform X13
XM_005165950.3	XP_005166007.1	kinesin-like protein KIF1A isoform X6
XM_005165953.3	XP_005166010.1	kinesin-like protein KIF1A isoform X9
XM_021477121.1	XP_021332796.1	kinesin-like protein KIF1A isoform X2
XM_005165955.3	XP_005166012.1	kinesin-like protein KIF1A isoform X17
XM_005165947.3	XP_005166004.1	kinesin-like protein KIF1A isoform X3
XM_009302426.2	XP_009300701.1	kinesin-like protein KIF1A isoform X14
XM_021477123.1	XP_021332798.1	kinesin-like protein KIF1A isoform X4
XM_021477130.1	XP_021332805.1	kinesin-like protein KIF1A isoform X12
XM_021477132.1	XP_021332807.1	kinesin-like protein KIF1A isoform X14
XM_009302425.2	XP_009300700.1	kinesin-like protein KIF1A isoform X16
XM_009302426.3	XP_009300701.1	kinesin-like protein KIF1A isoform X19
XM_005165945.3	XP_005166002.1	kinesin-like protein KIF1A isoform X1
XM_005165948.3	XP_005166005.1	kinesin-like protein KIF1A isoform X4
XM_005165951.3	XP_005166008.1	kinesin-like protein KIF1A isoform X7
XM_021477124.1	XP_021332799.1	kinesin-like protein KIF1A isoform X5
XM_002662438.5	XP_002662484.2	kinesin-like protein KIF1A isoform X18

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143065	XM_021477120.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143066	XM_021477121.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143067	XM_021477122.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143068	XM_021477123.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143069	XM_021477124.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143070	XM_021477125.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143071	XM_021477127.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143072	XM_021477128.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143073	XM_021477130.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143074	XM_021477131.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143075	XM_021477132.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa49317	XM_021477129.1 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa11872	XM_005165945.4 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11873	XM_005165946.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11874	XM_005165947.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11875	XM_005165948.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11876	XM_005165949.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11877	XM_005165950.4 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11878	XM_005165951.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11879	XM_005165952.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11880	XM_005165953.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11881	XM_005165954.4 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11882	XM_005165955.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11883	XM_002662438.5 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49318	XM_009302425.2 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49319	XM_009302426.3 Latest version!	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa11881	XM_005165954.3	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11877	XM_005165950.3	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49319	XM_009302426.2	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11872	XM_005165945.3	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

kif1aa ( XM_021477120.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332795.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143065
Clone ID Related Accession (Same CDS sequence)	XM_021477120.1
Accession Version	XM_021477120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGACA GTTTCTTGAT TCGGACATGC CTAGGACATT CTACCGGTTT 
GAGGCTGCTT GGGATTCATC CATGCACAAC TCTTTGCTGC TAAACCGTGT GACTCCTTAC 
GGAGAGAAAA TCTACATGAC TCTGTCTGCT TACCTGGAGA TGGAGAACTG CACACAACCC 
ACAGTGATTA CCAAAGACTT ATGCATGGTG TTCCACTCAA GAGACGCCAA ACTTCCAGCA 
TCTCGCTCCA TCAGAAACCT CTTCAGCAGT GGCACCTTAA GGCCGTCTGA AGGAAACCGT 
GTAACCGGTG TGTATGAGCT CAGTTTATGC CACCTGGCTG ATGCAGGGAG CCCAGGAATG 
CAAAGGCGGC GCAGGAGGGT GCTGGACACG TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC 
CTGGCTGGCT GGCGGCCCCG CAGTGACAGC CTCATCCTGG ACCATCAGTG GGAGCTGGAG 
AAATTGAGCC TCCTGCAGGA GGTGGAGAAA ACCAGACACT ACCTCCTCCT TAGGGAGAAG 
CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG TCGCTGCTGT CCTGCGGGAG TGAGGATCTA 
ACCGAGTCCT CTTCCCCCTC CACTCACCTC GGCCTGGGCC ACAACCCCGG CCCCGGCCCC 
GACACACCCA ATGAGAGGCA GAGGGAGCTG GCTGCTAAGT GTCTGCGTTT GCTTAACCAC 
ACATTTAACA GGGACTACAG CCATGTGTGT GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG 
ATGTCAGTGA CTCTCCTGAA AGACTCTGCT TCAGTGTGTG CCGAAAGCAC CCTCACACCC 
TCCTCCACAT GTCCTTCACT TGTGGAGAGT CGATACATTC ACAACACTGA TCTCAGACCC 
CCAACACCCC GATCTCGTGC TGTGAGCCCT AATCCTGACT CTGAAACAGA TGGTAAGAAG 
ATTCAAGAGC CCAGATCAAG AACCCGCACT TTTGTGCCAG ACATTCAGGA AATCCGTGTC 
AGCCCAATTG TGTCAAAGAA AGGCTATTTG CACTTTCTGG AGCCCCACAG TAATGGCTGG 
GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT TATGTCTACA TCTACAACTC AGACAGAGAC 
ACAGTGGAAA GGGCCATTCT TAACCTGTCC TCAGCGCAAG TGGAGTATAG CGAAGACCAG 
CAGGCCATGC TAAAGACCCC CTACACATTT GCGGTATGCA CAGAGCATCG TGGGATACTC 
CTTCAAGCCA GTAATGACAA GGAGATGCAT GACTGGCTGT ATGCCTTTAA CCCTCTGCTT 
GCTGGATCTA TCAGGTCTAA ACTGTCCAGA AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332795.1
CDS	352..6048
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477120.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477120.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGACA GTTTCTTGAT TCGGACATGC CTAGGACATT CTACCGGTTT 
GAGGCTGCTT GGGATTCATC CATGCACAAC TCTTTGCTGC TAAACCGTGT GACTCCTTAC 
GGAGAGAAAA TCTACATGAC TCTGTCTGCT TACCTGGAGA TGGAGAACTG CACACAACCC 
ACAGTGATTA CCAAAGACTT ATGCATGGTG TTCCACTCAA GAGACGCCAA ACTTCCAGCA 
TCTCGCTCCA TCAGAAACCT CTTCAGCAGT GGCACCTTAA GGCCGTCTGA AGGAAACCGT 
GTAACCGGTG TGTATGAGCT CAGTTTATGC CACCTGGCTG ATGCAGGGAG CCCAGGAATG 
CAAAGGCGGC GCAGGAGGGT GCTGGACACG TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC 
CTGGCTGGCT GGCGGCCCCG CAGTGACAGC CTCATCCTGG ACCATCAGTG GGAGCTGGAG 
AAATTGAGCC TCCTGCAGGA GGTGGAGAAA ACCAGACACT ACCTCCTCCT TAGGGAGAAG 
CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG TCGCTGCTGT CCTGCGGGAG TGAGGATCTA 
ACCGAGTCCT CTTCCCCCTC CACTCACCTC GGCCTGGGCC ACAACCCCGG CCCCGGCCCC 
GACACACCCA ATGAGAGGCA GAGGGAGCTG GCTGCTAAGT GTCTGCGTTT GCTTAACCAC 
ACATTTAACA GGGACTACAG CCATGTGTGT GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG 
ATGTCAGTGA CTCTCCTGAA AGACTCTGCT TCAGTGTGTG CCGAAAGCAC CCTCACACCC 
TCCTCCACAT GTCCTTCACT TGTGGAGAGT CGATACATTC ACAACACTGA TCTCAGACCC 
CCAACACCCC GATCTCGTGC TGTGAGCCCT AATCCTGACT CTGAAACAGA TGGTAAGAAG 
ATTCAAGAGC CCAGATCAAG AACCCGCACT TTTGTGCCAG ACATTCAGGA AATCCGTGTC 
AGCCCAATTG TGTCAAAGAA AGGCTATTTG CACTTTCTGG AGCCCCACAG TAATGGCTGG 
GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT TATGTCTACA TCTACAACTC AGACAGAGAC 
ACAGTGGAAA GGGCCATTCT TAACCTGTCC TCAGCGCAAG TGGAGTATAG CGAAGACCAG 
CAGGCCATGC TAAAGACCCC CTACACATTT GCGGTATGCA CAGAGCATCG TGGGATACTC 
CTTCAAGCCA GTAATGACAA GGAGATGCAT GACTGGCTGT ATGCCTTTAA CCCTCTGCTT 
GCTGGATCTA TCAGGTCTAA ACTGTCCAGA AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332796.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.

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CloneID	ODa143066
Clone ID Related Accession (Same CDS sequence)	XM_021477121.1
Accession Version	XM_021477121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGCAT TTCCTTGGGG ACTGATTCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332796.1
CDS	352..6045
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDSISLGTDSRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477121.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477121.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGCAT TTCCTTGGGG ACTGATTCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477122.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332797.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.

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CloneID	ODa143067
Clone ID Related Accession (Same CDS sequence)	XM_021477122.1
Accession Version	XM_021477122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG CCGCAGATAA CGACACCCCT CCAGATCGGG AGAAGAACCT GGAGCTGATT 
CGTTACCTGC TGCCTGAGGT CTTCAATGGT TCTTTGGTGG ACTCGCTGTC AAGCCACGCC 
CTCAATGCAG CAGGCGTGGC TGTTTCCTAC CTCCTTGAGG GGGAGGAGCA AGCCAGACTG 
CCAGCTCCGC CCCTGTCTAT CTGTTCTGTA ATAAAAGCGC AGAAGCCGGT CCCTGCTACC 
AAACTGACAG CCCTCACTCG ACCCACAGCA GGACCATGCC ACTGTAAATA TGACCTGATG 
GTCTTCTTTG AGATCTGCGA GCTGGAGGCT AATGGAGACT ATATCCCAGC CGTAGTGGAC 
CACCGAGTAG GCATGCCTTG CCATGGCACA TTCATGTTAC ATCAGGGCAT CCAAAGGAGA 
GTTACAGTCA CCATGGTACA TGAGACAGGA AGAGACATTG ACTGGAAGGA AGTGCGAGAG 
CTGGTTGTAG GACGTATCCG GAACACGCCT GAAGCAGATG AGGCAATCAT CGACCCCAAT 
ATTCTCTCTC TGAACATACT GTCTGCAGGA TACATTCGAC CCGCGCATGA TGACAGACAG 
TTTCTTGATT CGGACATGCC TAGGACATTC TACCGGTTTG AGGCTGCTTG GGATTCATCC 
ATGCACAACT CTTTGCTGCT AAACCGTGTG ACTCCTTACG GAGAGAAAAT CTACATGACT 
CTGTCTGCTT ACCTGGAGAT GGAGAACTGC ACACAACCCA CAGTGATTAC CAAAGACTTA 
TGCATGGTGT TCCACTCAAG AGACGCCAAA CTTCCAGCAT CTCGCTCCAT CAGAAACCTC 
TTCAGCAGTG GCACCTTAAG GCCGTCTGAA GGAAACCGTG TAACCGGTGT GTATGAGCTC 
AGTTTATGCC ACCTGGCTGA TGCAGGGAGC CCAGGAATGC AAAGGCGGCG CAGGAGGGTG 
CTGGACACGT CGGTGGCTTA TGTGCGAGGG GAGGAGAACC TGGCTGGCTG GCGGCCCCGC 
AGTGACAGCC TCATCCTGGA CCATCAGTGG GAGCTGGAGA AATTGAGCCT CCTGCAGGAG 
GTGGAGAAAA CCAGACACTA CCTCCTCCTT AGGGAGAAGC TGGAGTCCAC CCTGCTGCTG 
GGCCAGGAGT CGCTGCTGTC CTGCGGGAGT GAGGATCTAA CCGAGTCCTC TTCCCCCTCC 
ACTCACCTCG GCCTGGGCCA CAACCCCGGC CCCGGCCCCG ACACACCCAA TGAGAGGCAG 
AGGGAGCTGG CTGCTAAGTG TCTGCGTTTG CTTAACCACA CATTTAACAG GGACTACAGC 
CATGTGTGTG TTAGCGCCAG TGAGAGCAAG TTGAGTGAGA TGTCAGTGAC TCTCCTGAAA 
GACTCTGCTT CAGTGTGTGC CGAAAGCACC CTCACACCCT CCTCCACATG TCCTTCACTT 
GTGGAGAGTC GATACATTCA CAACACTGAT CTCAGACCCC CAACACCCCG ATCTCGTGCT 
GTGAGCCCTA ATCCTGACTC TGAAACAGAT GGTAAGAAGA TTCAAGAGCC CAGATCAAGA 
ACCCGCACTT TTGTGCCAGA CATTCAGGAA ATCCGTGTCA GCCCAATTGT GTCAAAGAAA 
GGCTATTTGC ACTTTCTGGA GCCCCACAGT AATGGCTGGG TTAAGAGATA TGTGGTGGTT 
CGTCGGCCTT ATGTCTACAT CTACAACTCA GACAGAGACA CAGTGGAAAG GGCCATTCTT 
AACCTGTCCT CAGCGCAAGT GGAGTATAGC GAAGACCAGC AGGCCATGCT AAAGACCCCC 
TACACATTTG CGGTATGCAC AGAGCATCGT GGGATACTCC TTCAAGCCAG TAATGACAAG 
GAGATGCATG ACTGGCTGTA TGCCTTTAAC CCTCTGCTTG CTGGATCTAT CAGGTCTAAA 
CTGTCCAGAA GGAGAGCTGG GCAGATGAGG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332797.1
CDS	352..6027
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477122.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477122.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG CCGCAGATAA CGACACCCCT CCAGATCGGG AGAAGAACCT GGAGCTGATT 
CGTTACCTGC TGCCTGAGGT CTTCAATGGT TCTTTGGTGG ACTCGCTGTC AAGCCACGCC 
CTCAATGCAG CAGGCGTGGC TGTTTCCTAC CTCCTTGAGG GGGAGGAGCA AGCCAGACTG 
CCAGCTCCGC CCCTGTCTAT CTGTTCTGTA ATAAAAGCGC AGAAGCCGGT CCCTGCTACC 
AAACTGACAG CCCTCACTCG ACCCACAGCA GGACCATGCC ACTGTAAATA TGACCTGATG 
GTCTTCTTTG AGATCTGCGA GCTGGAGGCT AATGGAGACT ATATCCCAGC CGTAGTGGAC 
CACCGAGTAG GCATGCCTTG CCATGGCACA TTCATGTTAC ATCAGGGCAT CCAAAGGAGA 
GTTACAGTCA CCATGGTACA TGAGACAGGA AGAGACATTG ACTGGAAGGA AGTGCGAGAG 
CTGGTTGTAG GACGTATCCG GAACACGCCT GAAGCAGATG AGGCAATCAT CGACCCCAAT 
ATTCTCTCTC TGAACATACT GTCTGCAGGA TACATTCGAC CCGCGCATGA TGACAGACAG 
TTTCTTGATT CGGACATGCC TAGGACATTC TACCGGTTTG AGGCTGCTTG GGATTCATCC 
ATGCACAACT CTTTGCTGCT AAACCGTGTG ACTCCTTACG GAGAGAAAAT CTACATGACT 
CTGTCTGCTT ACCTGGAGAT GGAGAACTGC ACACAACCCA CAGTGATTAC CAAAGACTTA 
TGCATGGTGT TCCACTCAAG AGACGCCAAA CTTCCAGCAT CTCGCTCCAT CAGAAACCTC 
TTCAGCAGTG GCACCTTAAG GCCGTCTGAA GGAAACCGTG TAACCGGTGT GTATGAGCTC 
AGTTTATGCC ACCTGGCTGA TGCAGGGAGC CCAGGAATGC AAAGGCGGCG CAGGAGGGTG 
CTGGACACGT CGGTGGCTTA TGTGCGAGGG GAGGAGAACC TGGCTGGCTG GCGGCCCCGC 
AGTGACAGCC TCATCCTGGA CCATCAGTGG GAGCTGGAGA AATTGAGCCT CCTGCAGGAG 
GTGGAGAAAA CCAGACACTA CCTCCTCCTT AGGGAGAAGC TGGAGTCCAC CCTGCTGCTG 
GGCCAGGAGT CGCTGCTGTC CTGCGGGAGT GAGGATCTAA CCGAGTCCTC TTCCCCCTCC 
ACTCACCTCG GCCTGGGCCA CAACCCCGGC CCCGGCCCCG ACACACCCAA TGAGAGGCAG 
AGGGAGCTGG CTGCTAAGTG TCTGCGTTTG CTTAACCACA CATTTAACAG GGACTACAGC 
CATGTGTGTG TTAGCGCCAG TGAGAGCAAG TTGAGTGAGA TGTCAGTGAC TCTCCTGAAA 
GACTCTGCTT CAGTGTGTGC CGAAAGCACC CTCACACCCT CCTCCACATG TCCTTCACTT 
GTGGAGAGTC GATACATTCA CAACACTGAT CTCAGACCCC CAACACCCCG ATCTCGTGCT 
GTGAGCCCTA ATCCTGACTC TGAAACAGAT GGTAAGAAGA TTCAAGAGCC CAGATCAAGA 
ACCCGCACTT TTGTGCCAGA CATTCAGGAA ATCCGTGTCA GCCCAATTGT GTCAAAGAAA 
GGCTATTTGC ACTTTCTGGA GCCCCACAGT AATGGCTGGG TTAAGAGATA TGTGGTGGTT 
CGTCGGCCTT ATGTCTACAT CTACAACTCA GACAGAGACA CAGTGGAAAG GGCCATTCTT 
AACCTGTCCT CAGCGCAAGT GGAGTATAGC GAAGACCAGC AGGCCATGCT AAAGACCCCC 
TACACATTTG CGGTATGCAC AGAGCATCGT GGGATACTCC TTCAAGCCAG TAATGACAAG 
GAGATGCATG ACTGGCTGTA TGCCTTTAAC CCTCTGCTTG CTGGATCTAT CAGGTCTAAA 
CTGTCCAGAA GGAGAGCTGG GCAGATGAGG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477123.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332798.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.

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CloneID	ODa143068
Clone ID Related Accession (Same CDS sequence)	XM_021477123.1
Accession Version	XM_021477123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGTGA GCAAGCCAGC GACCCCAACT CCTCCCCCAC CCCCTCTGAA 
CCCACCTCCA CCTCTGAACT CACCGAGCTC GTCCAGTCAT GGCCAGACAA GAAGGTGGGG 
ACAGAGCTGG ATGACGATGG TTTCTTATCA GACGACCCGT GCTCGGATTT AGAGGAGGAG 
GGGGAGGATG ATGATGATGA TGAGGTTGAT GATGACGAAG AGCTCTGCAG AAGCTCCGGC 
AAAGCTGAGG ACCCGTTTTA TGATCGTTCA CCATTGTTCA GTGTAGTAGG AAGGGCTTTT 
GTCTATTTGA GTAATCTGCT GTATCCTGTG CCTCTGGTCC ATCGGGTTGC CATCGTCAAT 
GAGAAGGGAG AAGTCAAAGG TTTCCTCAGA GTAGCTGTAC AGGCAATATC AGGTGCTCTG 
GTTTTTCCTC CAGCCGATGA AGAGGCACCA GACTATGGCT CTGGTGTCAG ACAGTCAGGC 
ACGGCCAAGA TTTCTTTTGA GGACCAGCAG TTTGAGAAGT TCCAGTCAGA GTCATGTCAT 
TCTGGTCTTT CGCGTGCTGC CGTTTCTCAG GAAGAGTTGA GGATTGTGGA GGGTGAAGGA 
CAAAGTGCAG AGATGGGACT GTCAGCAGAT GAGGTCAACA ATAATACATG TGCAGCTAAC 
CCAGAGGAGC TGGACAGCCC TGTCAAAACA GGTCTTGATG GGATGCTGGA CGGCGCTCTG 
GATCACTTGA GGATTGGTGA AGTCTTCACA TTCAGAGTTA CTGTTCTACA GGCGTCAAGC 
ATTTCCGCAG AGTACGCAGA CATATTCTGC CAGTTCAATT TTATTCACAG GCACGATGAA 
GCATTTTCCA CCGAGCCGTT GAAGAACACT GGACGTGGTC CACCACTTGG CTTCTATCAT 
GTGCAGAATA TTGCAGTGGA AGTGACTAAG TCCTTTATTG AGTACATAAA AACTCAGCCA 
ATCGTGTTTG AAGTCTTTGG ACACTACCAG AAGCAGCCTT TCCCTGCGCT CTGCAAGGAT 
CTCATTAGCC CATTGCGTCC ATGCAGACGA CAGTTCCCAA GGGTCATGCC TCTTTCCAAA 
CCAGCCGCAG ATAACGACAC CCCTCCAGAT CGGGAGAAGA ACCTGGAGCT GATTCGTTAC 
CTGCTGCCTG AGGTCTTCAA TGGTTCTTTG GTGGACTCGC TGTCAAGCCA CGCCCTCAAT 
GCAGCAGGCG TGGCTGTTTC CTACCTCCTT GAGGGGGAGG AGCAAGCCAG ACTGCCAGCT 
CCGCCCCTGT CTATCTGTTC TGTAATAAAA GCGCAGAAGC CGGTCCCTGC TACCAAACTG 
ACAGCCCTCA CTCGACCCAC AGCAGGACCA TGCCACTGTA AATATGACCT GATGGTCTTC 
TTTGAGATCT GCGAGCTGGA GGCTAATGGA GACTATATCC CAGCCGTAGT GGACCACCGA 
GTAGGCATGC CTTGCCATGG CACATTCATG TTACATCAGG GCATCCAAAG GAGAGTTACA 
GTCACCATGG TACATGAGAC AGGAAGAGAC ATTGACTGGA AGGAAGTGCG AGAGCTGGTT 
GTAGGACGTA TCCGGAACAC GCCTGAAGCA GATGAGGCAA TCATCGACCC CAATATTCTC 
TCTCTGAACA TACTGTCTGC AGGATACATT CGACCCGCGC ATGATGACAG ACAGTTTCTT 
GATTCGGACA TGCCTAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332798.1
CDS	352..6021
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477123.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477123.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGTGA GCAAGCCAGC GACCCCAACT CCTCCCCCAC CCCCTCTGAA 
CCCACCTCCA CCTCTGAACT CACCGAGCTC GTCCAGTCAT GGCCAGACAA GAAGGTGGGG 
ACAGAGCTGG ATGACGATGG TTTCTTATCA GACGACCCGT GCTCGGATTT AGAGGAGGAG 
GGGGAGGATG ATGATGATGA TGAGGTTGAT GATGACGAAG AGCTCTGCAG AAGCTCCGGC 
AAAGCTGAGG ACCCGTTTTA TGATCGTTCA CCATTGTTCA GTGTAGTAGG AAGGGCTTTT 
GTCTATTTGA GTAATCTGCT GTATCCTGTG CCTCTGGTCC ATCGGGTTGC CATCGTCAAT 
GAGAAGGGAG AAGTCAAAGG TTTCCTCAGA GTAGCTGTAC AGGCAATATC AGGTGCTCTG 
GTTTTTCCTC CAGCCGATGA AGAGGCACCA GACTATGGCT CTGGTGTCAG ACAGTCAGGC 
ACGGCCAAGA TTTCTTTTGA GGACCAGCAG TTTGAGAAGT TCCAGTCAGA GTCATGTCAT 
TCTGGTCTTT CGCGTGCTGC CGTTTCTCAG GAAGAGTTGA GGATTGTGGA GGGTGAAGGA 
CAAAGTGCAG AGATGGGACT GTCAGCAGAT GAGGTCAACA ATAATACATG TGCAGCTAAC 
CCAGAGGAGC TGGACAGCCC TGTCAAAACA GGTCTTGATG GGATGCTGGA CGGCGCTCTG 
GATCACTTGA GGATTGGTGA AGTCTTCACA TTCAGAGTTA CTGTTCTACA GGCGTCAAGC 
ATTTCCGCAG AGTACGCAGA CATATTCTGC CAGTTCAATT TTATTCACAG GCACGATGAA 
GCATTTTCCA CCGAGCCGTT GAAGAACACT GGACGTGGTC CACCACTTGG CTTCTATCAT 
GTGCAGAATA TTGCAGTGGA AGTGACTAAG TCCTTTATTG AGTACATAAA AACTCAGCCA 
ATCGTGTTTG AAGTCTTTGG ACACTACCAG AAGCAGCCTT TCCCTGCGCT CTGCAAGGAT 
CTCATTAGCC CATTGCGTCC ATGCAGACGA CAGTTCCCAA GGGTCATGCC TCTTTCCAAA 
CCAGCCGCAG ATAACGACAC CCCTCCAGAT CGGGAGAAGA ACCTGGAGCT GATTCGTTAC 
CTGCTGCCTG AGGTCTTCAA TGGTTCTTTG GTGGACTCGC TGTCAAGCCA CGCCCTCAAT 
GCAGCAGGCG TGGCTGTTTC CTACCTCCTT GAGGGGGAGG AGCAAGCCAG ACTGCCAGCT 
CCGCCCCTGT CTATCTGTTC TGTAATAAAA GCGCAGAAGC CGGTCCCTGC TACCAAACTG 
ACAGCCCTCA CTCGACCCAC AGCAGGACCA TGCCACTGTA AATATGACCT GATGGTCTTC 
TTTGAGATCT GCGAGCTGGA GGCTAATGGA GACTATATCC CAGCCGTAGT GGACCACCGA 
GTAGGCATGC CTTGCCATGG CACATTCATG TTACATCAGG GCATCCAAAG GAGAGTTACA 
GTCACCATGG TACATGAGAC AGGAAGAGAC ATTGACTGGA AGGAAGTGCG AGAGCTGGTT 
GTAGGACGTA TCCGGAACAC GCCTGAAGCA GATGAGGCAA TCATCGACCC CAATATTCTC 
TCTCTGAACA TACTGTCTGC AGGATACATT CGACCCGCGC ATGATGACAG ACAGTTTCTT 
GATTCGGACA TGCCTAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332799.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.

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CloneID	ODa143069
Clone ID Related Accession (Same CDS sequence)	XM_021477124.1
Accession Version	XM_021477124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332799.1
CDS	352..6021
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477124.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477124.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GCCGCAGATA ACGACACCCC TCCAGATCGG 
GAGAAGAACC TGGAGCTGAT TCGTTACCTG CTGCCTGAGG TCTTCAATGG TTCTTTGGTG 
GACTCGCTGT CAAGCCACGC CCTCAATGCA GCAGGCGTGG CTGTTTCCTA CCTCCTTGAG 
GGGGAGGAGC AAGCCAGACT GCCAGCTCCG CCCCTGTCTA TCTGTTCTGT AATAAAAGCG 
CAGAAGCCGG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477125.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332800.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.

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CloneID	ODa143070
Clone ID Related Accession (Same CDS sequence)	XM_021477125.1
Accession Version	XM_021477125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA ACATATCCGA TTACAAGAAC 
CATAACAATA ATGGCCAAGC TCTCAATCAA AGAGGTGATT TCTCCACAGT GACCAATGCT 
ATGACTGGGA TGAGCCCCTC TCCATCACTC TCTGCCCTGT CCAGCCGGGC CGGTTCCATT 
AGCAGCCTGC ACGATCGCAT CATGTTCAGC CCCGGGAGCG AAGAAGCCAT TGAGAGACTC 
AAGGAAACTG AGAAAATCAT TGCAGAGCTC AATGAAACAT GGGAGGAGAA GCTTCGCCGT 
ACAGAGGCCA TCAGGATGGA CAGAGAAGCA CTTCTGGCGG AGATGGGTGT TGCCATGCGT 
GAGGATGGAG GAACTCTTGG AGTCTTTTCC CCAAAAAAGA CTCCCCACCT AGTGAATCTC 
AATGAAGATC CCTTGATGTC TGAATGTCTT CTATACTACA TCAAAGATGG GATTACCAGG 
GTGGGCAGAG AAGATGCCAG TAGTCGGCAG GACATTGTTC TGAGCGGACA CTTCATTAAG 
GAGGAACATT GTACTTTCAC CAGCACCACT GGACCAATGG GAGAAGCTGT CATTCTTGAG 
CCATGTGAGG GAGCAGAAAC ATATGTAAAT GGCAAGAGAG TCACAGAACC CACTGTTCTC 
AAATCAGGCA ACCGAATCAT TTTGGGCAAG AGCCACGTGT TCCGCTTCAA CCATCCAGAA 
CAGGCCCGTC AGGAACGTGA CCGAACCCCC TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC 
TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT AAACAGGGCA TTGACATGAA GCAGGAGATG 
GATCAGAGAC TGCAGGAACT TGAAGATCAG TATCGCAAGG AGAGAGAAGA GGCCAACAAT 
CTACTGGAGC AACAGAGACT AGATTATGAG AGCAGGTTAG AGGCTCTGCA GAAACAGGTC 
GATCGATGTT ATCCTGACGC AGCAGAGGAA GATGAAGAGC CTGAGGAGGA AGTGCAATGG 
ACGGAGAGGG AGACGGAACT GGCAATGTGG GCTTTCCGCA AATGGAGATG TTATCAGTTT 
ACATCCCTTC GAGATCTTCT TTGGGGCAAT GCCATCTTCC TTAAAGAGGC CAATGCCATT 
AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC CAGTTCGTGT TATTGACGGA CACACTTTAC 
TCTCCTCTTC CGCCTGACCT GTTACCGCCT GAAGCAGCTA AAGACAGAGA GAAAAGCCCA 
TTTCCACGCA CCATTGTAGC TGTTGAAGTT CAAGATCAGA AAAACGGAGC CACGCATTAC 
TGGACCCTGG AAAAGCTCAG GCAGAGACTG GATCTGATGA GAGAGATGTA TGATCGAGCT 
GCTGAGGTGC CCAGCAATGC TGTTGAAGAC TGTGACAGTG TGCTGACCGG AGGAGATCCT 
TTCTACGACC GCTTTCCCTG GTTTCGTCTG GTTGGAAGAA ATCCCCTTTT CTCCACATGC 
CTTAGTGAGC AAGCCAGCGA CCCCAACTCC TCCCCCACCC CCTCTGAACC CACCTCCACC 
TCTGAACTCA CCGAGCTCGT CCAGTCATGG CCAGACAAGA AGGTGGGGAC AGAGCTGGAT 
GACGATGGTT TCTTATCAGA CGACCCGTGC TCGGATTTAG AGGAGGAGGG GGAGGATGAT 
GATGATGATG AGGTTGATGA TGACGAAGAG CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC 
CCGTTTTATG ATCGTTCACC ATTGTTCAGT GTAGTAGGAA GGGCTTTTGT CTATTTGAGT 
AATCTGCTGT ATCCTGTGCC TCTGGTCCAT CGGGTTGCCA TCGTCAATGA GAAGGGAGAA 
GTCAAAGGTT TCCTCAGAGT AGCTGTACAG GCAATATCAG GTGCTCTGGT TTTTCCTCCA 
GCCGATGAAG AGGCACCAGA CTATGGCTCT GGTGTCAGAC AGTCAGGCAC GGCCAAGATT 
TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC CAGTCAGAGT CATGTCATTC TGGTCTTTCG 
CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG ATTGTGGAGG GTGAAGGACA AAGTGCAGAG 
ATGGGACTGT CAGCAGATGA GGTCAACAAT AATACATGTG CAGCTAACCC AGAGGAGCTG 
GACAGCCCTG TCAAAACAGG TCTTGATGGG ATGCTGGACG GCGCTCTGGA TCACTTGAGG 
ATTGGTGAAG TCTTCACATT CAGAGTTACT GTTCTACAGG CGTCAAGCAT TTCCGCAGAG 
TACGCAGACA TATTCTGCCA GTTCAATTTT ATTCACAGGC ACGATGAAGC ATTTTCCACC 
GAGCCGTTGA AGAACACTGG ACGTGGTCCA CCACTTGGCT TCTATCATGT GCAGAATATT 
GCAGTGGAAG TGACTAAGTC CTTTATTGAG TACATAAAAA CTCAGCCAAT CGTGTTTGAA 
GTCTTTGGAC ACTACCAGAA GCAGCCTTTC CCTGCGCTCT GCAAGGATCT CATTAGCCCA 
TTGCGTCCAT GCAGACGACA GTTCCCAAGG GTCATGCCTC TTTCCAAACC AGCCGCAGAT 
AACGACACCC CTCCAGATCG GGAGAAGAAC CTGGAGCTGA TTCGTTACCT GCTGCCTGAG 
GTCTTCAATG GTTCTTTGGT GGACTCGCTG TCAAGCCACG CCCTCAATGC AGCAGGCGTG 
GCTGTTTCCT ACCTCCTTGA GGGGGAGGAG CAAGCCAGAC TGCCAGCTCC GCCCCTGTCT 
ATCTGTTCTG TAATAAAAGC GCAGAAGCCG GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332800.1
CDS	352..6009
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIENISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477125.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477125.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA ACATATCCGA TTACAAGAAC 
CATAACAATA ATGGCCAAGC TCTCAATCAA AGAGGTGATT TCTCCACAGT GACCAATGCT 
ATGACTGGGA TGAGCCCCTC TCCATCACTC TCTGCCCTGT CCAGCCGGGC CGGTTCCATT 
AGCAGCCTGC ACGATCGCAT CATGTTCAGC CCCGGGAGCG AAGAAGCCAT TGAGAGACTC 
AAGGAAACTG AGAAAATCAT TGCAGAGCTC AATGAAACAT GGGAGGAGAA GCTTCGCCGT 
ACAGAGGCCA TCAGGATGGA CAGAGAAGCA CTTCTGGCGG AGATGGGTGT TGCCATGCGT 
GAGGATGGAG GAACTCTTGG AGTCTTTTCC CCAAAAAAGA CTCCCCACCT AGTGAATCTC 
AATGAAGATC CCTTGATGTC TGAATGTCTT CTATACTACA TCAAAGATGG GATTACCAGG 
GTGGGCAGAG AAGATGCCAG TAGTCGGCAG GACATTGTTC TGAGCGGACA CTTCATTAAG 
GAGGAACATT GTACTTTCAC CAGCACCACT GGACCAATGG GAGAAGCTGT CATTCTTGAG 
CCATGTGAGG GAGCAGAAAC ATATGTAAAT GGCAAGAGAG TCACAGAACC CACTGTTCTC 
AAATCAGGCA ACCGAATCAT TTTGGGCAAG AGCCACGTGT TCCGCTTCAA CCATCCAGAA 
CAGGCCCGTC AGGAACGTGA CCGAACCCCC TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC 
TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT AAACAGGGCA TTGACATGAA GCAGGAGATG 
GATCAGAGAC TGCAGGAACT TGAAGATCAG TATCGCAAGG AGAGAGAAGA GGCCAACAAT 
CTACTGGAGC AACAGAGACT AGATTATGAG AGCAGGTTAG AGGCTCTGCA GAAACAGGTC 
GATCGATGTT ATCCTGACGC AGCAGAGGAA GATGAAGAGC CTGAGGAGGA AGTGCAATGG 
ACGGAGAGGG AGACGGAACT GGCAATGTGG GCTTTCCGCA AATGGAGATG TTATCAGTTT 
ACATCCCTTC GAGATCTTCT TTGGGGCAAT GCCATCTTCC TTAAAGAGGC CAATGCCATT 
AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC CAGTTCGTGT TATTGACGGA CACACTTTAC 
TCTCCTCTTC CGCCTGACCT GTTACCGCCT GAAGCAGCTA AAGACAGAGA GAAAAGCCCA 
TTTCCACGCA CCATTGTAGC TGTTGAAGTT CAAGATCAGA AAAACGGAGC CACGCATTAC 
TGGACCCTGG AAAAGCTCAG GCAGAGACTG GATCTGATGA GAGAGATGTA TGATCGAGCT 
GCTGAGGTGC CCAGCAATGC TGTTGAAGAC TGTGACAGTG TGCTGACCGG AGGAGATCCT 
TTCTACGACC GCTTTCCCTG GTTTCGTCTG GTTGGAAGAA ATCCCCTTTT CTCCACATGC 
CTTAGTGAGC AAGCCAGCGA CCCCAACTCC TCCCCCACCC CCTCTGAACC CACCTCCACC 
TCTGAACTCA CCGAGCTCGT CCAGTCATGG CCAGACAAGA AGGTGGGGAC AGAGCTGGAT 
GACGATGGTT TCTTATCAGA CGACCCGTGC TCGGATTTAG AGGAGGAGGG GGAGGATGAT 
GATGATGATG AGGTTGATGA TGACGAAGAG CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC 
CCGTTTTATG ATCGTTCACC ATTGTTCAGT GTAGTAGGAA GGGCTTTTGT CTATTTGAGT 
AATCTGCTGT ATCCTGTGCC TCTGGTCCAT CGGGTTGCCA TCGTCAATGA GAAGGGAGAA 
GTCAAAGGTT TCCTCAGAGT AGCTGTACAG GCAATATCAG GTGCTCTGGT TTTTCCTCCA 
GCCGATGAAG AGGCACCAGA CTATGGCTCT GGTGTCAGAC AGTCAGGCAC GGCCAAGATT 
TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC CAGTCAGAGT CATGTCATTC TGGTCTTTCG 
CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG ATTGTGGAGG GTGAAGGACA AAGTGCAGAG 
ATGGGACTGT CAGCAGATGA GGTCAACAAT AATACATGTG CAGCTAACCC AGAGGAGCTG 
GACAGCCCTG TCAAAACAGG TCTTGATGGG ATGCTGGACG GCGCTCTGGA TCACTTGAGG 
ATTGGTGAAG TCTTCACATT CAGAGTTACT GTTCTACAGG CGTCAAGCAT TTCCGCAGAG 
TACGCAGACA TATTCTGCCA GTTCAATTTT ATTCACAGGC ACGATGAAGC ATTTTCCACC 
GAGCCGTTGA AGAACACTGG ACGTGGTCCA CCACTTGGCT TCTATCATGT GCAGAATATT 
GCAGTGGAAG TGACTAAGTC CTTTATTGAG TACATAAAAA CTCAGCCAAT CGTGTTTGAA 
GTCTTTGGAC ACTACCAGAA GCAGCCTTTC CCTGCGCTCT GCAAGGATCT CATTAGCCCA 
TTGCGTCCAT GCAGACGACA GTTCCCAAGG GTCATGCCTC TTTCCAAACC AGCCGCAGAT 
AACGACACCC CTCCAGATCG GGAGAAGAAC CTGGAGCTGA TTCGTTACCT GCTGCCTGAG 
GTCTTCAATG GTTCTTTGGT GGACTCGCTG TCAAGCCACG CCCTCAATGC AGCAGGCGTG 
GCTGTTTCCT ACCTCCTTGA GGGGGAGGAG CAAGCCAGAC TGCCAGCTCC GCCCCTGTCT 
ATCTGTTCTG TAATAAAAGC GCAGAAGCCG GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477127.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332802.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa143071
Clone ID Related Accession (Same CDS sequence)	XM_021477127.1
Accession Version	XM_021477127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATT GACCAATGCT ATGACTGGGA TGAGCCCCTC TCCATCACTC 
TCTGCCCTGT CCAGCCGGGC CGGTTCCATT AGCAGCCTGC ACGATCGCAT CATGTTCAGC 
CCCGGGAGCG AAGAAGCCAT TGAGAGACTC AAGGAAACTG AGAAAATCAT TGCAGAGCTC 
AATGAAACAT GGGAGGAGAA GCTTCGCCGT ACAGAGGCCA TCAGGATGGA CAGAGAAGCA 
CTTCTGGCGG AGATGGGTGT TGCCATGCGT GAGGATGGAG GAACTCTTGG AGTCTTTTCC 
CCAAAAAAGA CTCCCCACCT AGTGAATCTC AATGAAGATC CCTTGATGTC TGAATGTCTT 
CTATACTACA TCAAAGATGG GATTACCAGG GTGGGCAGAG AAGATGCCAG TAGTCGGCAG 
GACATTGTTC TGAGCGGACA CTTCATTAAG GAGGAACATT GTACTTTCAC CAGCACCACT 
GGACCAATGG GAGAAGCTGT CATTCTTGAG CCATGTGAGG GAGCAGAAAC ATATGTAAAT 
GGCAAGAGAG TCACAGAACC CACTGTTCTC AAATCAGGCA ACCGAATCAT TTTGGGCAAG 
AGCCACGTGT TCCGCTTCAA CCATCCAGAA CAGGCCCGTC AGGAACGTGA CCGAACCCCC 
TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT 
AAACAGGGCA TTGACATGAA GCAGGAGATG GATCAGAGAC TGCAGGAACT TGAAGATCAG 
TATCGCAAGG AGAGAGAAGA GGCCAACAAT CTACTGGAGC AACAGAGACT AGATTATGAG 
AGCAGGTTAG AGGCTCTGCA GAAACAGGTC GATCGATGTT ATCCTGACGC AGCAGAGGAA 
GATGAAGAGC CTGAGGAGGA AGTGCAATGG ACGGAGAGGG AGACGGAACT GGCAATGTGG 
GCTTTCCGCA AATGGAGATG TTATCAGTTT ACATCCCTTC GAGATCTTCT TTGGGGCAAT 
GCCATCTTCC TTAAAGAGGC CAATGCCATT AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC 
CAGTTCGTGT TATTGACGGA CACACTTTAC TCTCCTCTTC CGCCTGACCT GTTACCGCCT 
GAAGCAGCTA AAGACAGAGA GAAAAGCCCA TTTCCACGCA CCATTGTAGC TGTTGAAGTT 
CAAGATCAGA AAAACGGAGC CACGCATTAC TGGACCCTGG AAAAGCTCAG GCAGAGACTG 
GATCTGATGA GAGAGATGTA TGATCGAGCT GCTGAGGTGC CCAGCAATGC TGTTGAAGAC 
TGTGACAGTG TGCTGACCGG AGGAGATCCT TTCTACGACC GCTTTCCCTG GTTTCGTCTG 
GTTGGAAGAA ATCCCCTTTT CTCCACATGC CTTAGTGAGC AAGCCAGCGA CCCCAACTCC 
TCCCCCACCC CCTCTGAACC CACCTCCACC TCTGAACTCA CCGAGCTCGT CCAGTCATGG 
CCAGACAAGA AGGTGGGGAC AGAGCTGGAT GACGATGGTT TCTTATCAGA CGACCCGTGC 
TCGGATTTAG AGGAGGAGGG GGAGGATGAT GATGATGATG AGGTTGATGA TGACGAAGAG 
CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC CCGTTTTATG ATCGTTCACC ATTGTTCAGT 
GTAGTAGGAA GGGCTTTTGT CTATTTGAGT AATCTGCTGT ATCCTGTGCC TCTGGTCCAT 
CGGGTTGCCA TCGTCAATGA GAAGGGAGAA GTCAAAGGTT TCCTCAGAGT AGCTGTACAG 
GCAATATCAG GTGCTCTGGT TTTTCCTCCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGCCGCAGAT AACGACACCC CTCCAGATCG GGAGAAGAAC 
CTGGAGCTGA TTCGTTACCT GCTGCCTGAG GTCTTCAATG GTTCTTTGGT GGACTCGCTG 
TCAAGCCACG CCCTCAATGC AGCAGGCGTG GCTGTTTCCT ACCTCCTTGA GGGGGAGGAG 
CAAGCCAGAC TGCCAGCTCC GCCCCTGTCT ATCTGTTCTG TAATAAAAGC GCAGAAGCCG 
GTCCCTGCTA CCAAACTGAC AGCCCTCACT CGACCCACAG CAGGACCATG CCACTGTAAA 
TATGACCTGA TGGTCTTCTT TGAGATCTGC GAGCTGGAGG CTAATGGAGA CTATATCCCA 
GCCGTAGTGG ACCACCGAGT AGGCATGCCT TGCCATGGCA CATTCATGTT ACATCAGGGC 
ATCCAAAGGA GAGTTACAGT CACCATGGTA CATGAGACAG GAAGAGACAT TGACTGGAAG 
GAAGTGCGAG AGCTGGTTGT AGGACGTATC CGGAACACGC CTGAAGCAGA TGAGGCAATC 
ATCGACCCCA ATATTCTCTC TCTGAACATA CTGTCTGCAG GATACATTCG ACCCGCGCAT 
GATGACAGAC AGTTTCTTGA TTCGGACATG CCTAGGACAT TCTACCGGTT TGAGGCTGCT 
TGGGATTCAT CCATGCACAA CTCTTTGCTG CTAAACCGTG TGACTCCTTA CGGAGAGAAA 
ATCTACATGA CTCTGTCTGC TTACCTGGAG ATGGAGAACT GCACACAACC CACAGTGATT 
ACCAAAGACT TATGCATGGT GTTCCACTCA AGAGACGCCA AACTTCCAGC ATCTCGCTCC 
ATCAGAAACC TCTTCAGCAG TGGCACCTTA AGGCCGTCTG AAGGAAACCG TGTAACCGGT 
GTGTATGAGC TCAGTTTATG CCACCTGGCT GATGCAGGGA GCCCAGGAAT GCAAAGGCGG 
CGCAGGAGGG TGCTGGACAC GTCGGTGGCT TATGTGCGAG GGGAGGAGAA CCTGGCTGGC 
TGGCGGCCCC GCAGTGACAG CCTCATCCTG GACCATCAGT GGGAGCTGGA GAAATTGAGC 
CTCCTGCAGG AGGTGGAGAA AACCAGACAC TACCTCCTCC TTAGGGAGAA GCTGGAGTCC 
ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG TCCTGCGGGA GTGAGGATCT AACCGAGTCC 
TCTTCCCCCT CCACTCACCT CGGCCTGGGC CACAACCCCG GCCCCGGCCC CGACACACCC 
AATGAGAGGC AGAGGGAGCT GGCTGCTAAG TGTCTGCGTT TGCTTAACCA CACATTTAAC 
AGGGACTACA GCCATGTGTG TGTTAGCGCC AGTGAGAGCA AGTTGAGTGA GATGTCAGTG 
ACTCTCCTGA AAGACTCTGC TTCAGTGTGT GCCGAAAGCA CCCTCACACC CTCCTCCACA 
TGTCCTTCAC TTGTGGAGAG TCGATACATT CACAACACTG ATCTCAGACC CCCAACACCC 
CGATCTCGTG CTGTGAGCCC TAATCCTGAC TCTGAAACAG ATGGTAAGAA GATTCAAGAG 
CCCAGATCAA GAACCCGCAC TTTTGTGCCA GACATTCAGG AAATCCGTGT CAGCCCAATT 
GTGTCAAAGA AAGGCTATTT GCACTTTCTG GAGCCCCACA GTAATGGCTG GGTTAAGAGA 
TATGTGGTGG TTCGTCGGCC TTATGTCTAC ATCTACAACT CAGACAGAGA CACAGTGGAA 
AGGGCCATTC TTAACCTGTC CTCAGCGCAA GTGGAGTATA GCGAAGACCA GCAGGCCATG 
CTAAAGACCC CCTACACATT TGCGGTATGC ACAGAGCATC GTGGGATACT CCTTCAAGCC 
AGTAATGACA AGGAGATGCA TGACTGGCTG TATGCCTTTA ACCCTCTGCT TGCTGGATCT 
ATCAGGTCTA AACTGTCCAG AAGGAGAGCT GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332802.1
CDS	352..5979
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKLTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477127.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477127.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATT GACCAATGCT ATGACTGGGA TGAGCCCCTC TCCATCACTC 
TCTGCCCTGT CCAGCCGGGC CGGTTCCATT AGCAGCCTGC ACGATCGCAT CATGTTCAGC 
CCCGGGAGCG AAGAAGCCAT TGAGAGACTC AAGGAAACTG AGAAAATCAT TGCAGAGCTC 
AATGAAACAT GGGAGGAGAA GCTTCGCCGT ACAGAGGCCA TCAGGATGGA CAGAGAAGCA 
CTTCTGGCGG AGATGGGTGT TGCCATGCGT GAGGATGGAG GAACTCTTGG AGTCTTTTCC 
CCAAAAAAGA CTCCCCACCT AGTGAATCTC AATGAAGATC CCTTGATGTC TGAATGTCTT 
CTATACTACA TCAAAGATGG GATTACCAGG GTGGGCAGAG AAGATGCCAG TAGTCGGCAG 
GACATTGTTC TGAGCGGACA CTTCATTAAG GAGGAACATT GTACTTTCAC CAGCACCACT 
GGACCAATGG GAGAAGCTGT CATTCTTGAG CCATGTGAGG GAGCAGAAAC ATATGTAAAT 
GGCAAGAGAG TCACAGAACC CACTGTTCTC AAATCAGGCA ACCGAATCAT TTTGGGCAAG 
AGCCACGTGT TCCGCTTCAA CCATCCAGAA CAGGCCCGTC AGGAACGTGA CCGAACCCCC 
TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT 
AAACAGGGCA TTGACATGAA GCAGGAGATG GATCAGAGAC TGCAGGAACT TGAAGATCAG 
TATCGCAAGG AGAGAGAAGA GGCCAACAAT CTACTGGAGC AACAGAGACT AGATTATGAG 
AGCAGGTTAG AGGCTCTGCA GAAACAGGTC GATCGATGTT ATCCTGACGC AGCAGAGGAA 
GATGAAGAGC CTGAGGAGGA AGTGCAATGG ACGGAGAGGG AGACGGAACT GGCAATGTGG 
GCTTTCCGCA AATGGAGATG TTATCAGTTT ACATCCCTTC GAGATCTTCT TTGGGGCAAT 
GCCATCTTCC TTAAAGAGGC CAATGCCATT AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC 
CAGTTCGTGT TATTGACGGA CACACTTTAC TCTCCTCTTC CGCCTGACCT GTTACCGCCT 
GAAGCAGCTA AAGACAGAGA GAAAAGCCCA TTTCCACGCA CCATTGTAGC TGTTGAAGTT 
CAAGATCAGA AAAACGGAGC CACGCATTAC TGGACCCTGG AAAAGCTCAG GCAGAGACTG 
GATCTGATGA GAGAGATGTA TGATCGAGCT GCTGAGGTGC CCAGCAATGC TGTTGAAGAC 
TGTGACAGTG TGCTGACCGG AGGAGATCCT TTCTACGACC GCTTTCCCTG GTTTCGTCTG 
GTTGGAAGAA ATCCCCTTTT CTCCACATGC CTTAGTGAGC AAGCCAGCGA CCCCAACTCC 
TCCCCCACCC CCTCTGAACC CACCTCCACC TCTGAACTCA CCGAGCTCGT CCAGTCATGG 
CCAGACAAGA AGGTGGGGAC AGAGCTGGAT GACGATGGTT TCTTATCAGA CGACCCGTGC 
TCGGATTTAG AGGAGGAGGG GGAGGATGAT GATGATGATG AGGTTGATGA TGACGAAGAG 
CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC CCGTTTTATG ATCGTTCACC ATTGTTCAGT 
GTAGTAGGAA GGGCTTTTGT CTATTTGAGT AATCTGCTGT ATCCTGTGCC TCTGGTCCAT 
CGGGTTGCCA TCGTCAATGA GAAGGGAGAA GTCAAAGGTT TCCTCAGAGT AGCTGTACAG 
GCAATATCAG GTGCTCTGGT TTTTCCTCCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGCCGCAGAT AACGACACCC CTCCAGATCG GGAGAAGAAC 
CTGGAGCTGA TTCGTTACCT GCTGCCTGAG GTCTTCAATG GTTCTTTGGT GGACTCGCTG 
TCAAGCCACG CCCTCAATGC AGCAGGCGTG GCTGTTTCCT ACCTCCTTGA GGGGGAGGAG 
CAAGCCAGAC TGCCAGCTCC GCCCCTGTCT ATCTGTTCTG TAATAAAAGC GCAGAAGCCG 
GTCCCTGCTA CCAAACTGAC AGCCCTCACT CGACCCACAG CAGGACCATG CCACTGTAAA 
TATGACCTGA TGGTCTTCTT TGAGATCTGC GAGCTGGAGG CTAATGGAGA CTATATCCCA 
GCCGTAGTGG ACCACCGAGT AGGCATGCCT TGCCATGGCA CATTCATGTT ACATCAGGGC 
ATCCAAAGGA GAGTTACAGT CACCATGGTA CATGAGACAG GAAGAGACAT TGACTGGAAG 
GAAGTGCGAG AGCTGGTTGT AGGACGTATC CGGAACACGC CTGAAGCAGA TGAGGCAATC 
ATCGACCCCA ATATTCTCTC TCTGAACATA CTGTCTGCAG GATACATTCG ACCCGCGCAT 
GATGACAGAC AGTTTCTTGA TTCGGACATG CCTAGGACAT TCTACCGGTT TGAGGCTGCT 
TGGGATTCAT CCATGCACAA CTCTTTGCTG CTAAACCGTG TGACTCCTTA CGGAGAGAAA 
ATCTACATGA CTCTGTCTGC TTACCTGGAG ATGGAGAACT GCACACAACC CACAGTGATT 
ACCAAAGACT TATGCATGGT GTTCCACTCA AGAGACGCCA AACTTCCAGC ATCTCGCTCC 
ATCAGAAACC TCTTCAGCAG TGGCACCTTA AGGCCGTCTG AAGGAAACCG TGTAACCGGT 
GTGTATGAGC TCAGTTTATG CCACCTGGCT GATGCAGGGA GCCCAGGAAT GCAAAGGCGG 
CGCAGGAGGG TGCTGGACAC GTCGGTGGCT TATGTGCGAG GGGAGGAGAA CCTGGCTGGC 
TGGCGGCCCC GCAGTGACAG CCTCATCCTG GACCATCAGT GGGAGCTGGA GAAATTGAGC 
CTCCTGCAGG AGGTGGAGAA AACCAGACAC TACCTCCTCC TTAGGGAGAA GCTGGAGTCC 
ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG TCCTGCGGGA GTGAGGATCT AACCGAGTCC 
TCTTCCCCCT CCACTCACCT CGGCCTGGGC CACAACCCCG GCCCCGGCCC CGACACACCC 
AATGAGAGGC AGAGGGAGCT GGCTGCTAAG TGTCTGCGTT TGCTTAACCA CACATTTAAC 
AGGGACTACA GCCATGTGTG TGTTAGCGCC AGTGAGAGCA AGTTGAGTGA GATGTCAGTG 
ACTCTCCTGA AAGACTCTGC TTCAGTGTGT GCCGAAAGCA CCCTCACACC CTCCTCCACA 
TGTCCTTCAC TTGTGGAGAG TCGATACATT CACAACACTG ATCTCAGACC CCCAACACCC 
CGATCTCGTG CTGTGAGCCC TAATCCTGAC TCTGAAACAG ATGGTAAGAA GATTCAAGAG 
CCCAGATCAA GAACCCGCAC TTTTGTGCCA GACATTCAGG AAATCCGTGT CAGCCCAATT 
GTGTCAAAGA AAGGCTATTT GCACTTTCTG GAGCCCCACA GTAATGGCTG GGTTAAGAGA 
TATGTGGTGG TTCGTCGGCC TTATGTCTAC ATCTACAACT CAGACAGAGA CACAGTGGAA 
AGGGCCATTC TTAACCTGTC CTCAGCGCAA GTGGAGTATA GCGAAGACCA GCAGGCCATG 
CTAAAGACCC CCTACACATT TGCGGTATGC ACAGAGCATC GTGGGATACT CCTTCAAGCC 
AGTAATGACA AGGAGATGCA TGACTGGCTG TATGCCTTTA ACCCTCTGCT TGCTGGATCT 
ATCAGGTCTA AACTGTCCAG AAGGAGAGCT GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477128.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332803.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa143072
Clone ID Related Accession (Same CDS sequence)	XM_021477128.1
Accession Version	XM_021477128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GGTGCTCTGG TTTTTCCTCC AGCCGATGAA 
GAGGCACCAG ACTATGGCTC TGGTGTCAGA CAGTCAGGCA CGGCCAAGAT TTCTTTTGAG 
GACCAGCAGT TTGAGAAGTT CCAGTCAGAG TCATGTCATT CTGGTCTTTC GCGTGCTGCC 
GTTTCTCAGG AAGAGTTGAG GATTGTGGAG GGTGAAGGAC AAAGTGCAGA GATGGGACTG 
TCAGCAGATG AGGTCAACAA TAATACATGT GCAGCTAACC CAGAGGAGCT GGACAGCCCT 
GTCAAAACAG GTCTTGATGG GATGCTGGAC GGCGCTCTGG ATCACTTGAG GATTGGTGAA 
GTCTTCACAT TCAGAGTTAC TGTTCTACAG GCGTCAAGCA TTTCCGCAGA GTACGCAGAC 
ATATTCTGCC AGTTCAATTT TATTCACAGG CACGATGAAG CATTTTCCAC CGAGCCGTTG 
AAGAACACTG GACGTGGTCC ACCACTTGGC TTCTATCATG TGCAGAATAT TGCAGTGGAA 
GTGACTAAGT CCTTTATTGA GTACATAAAA ACTCAGCCAA TCGTGTTTGA AGTCTTTGGA 
CACTACCAGA AGCAGCCTTT CCCTGCGCTC TGCAAGGATC TCATTAGCCC ATTGCGTCCA 
TGCAGACGAC AGTTCCCAAG GGTCATGCCT CTTTCCAAAC CAGCCGCAGA TAACGACACC 
CCTCCAGATC GGGAGAAGAA CCTGGAGCTG ATTCGTTACC TGCTGCCTGA GGTCTTCAAT 
GGTTCTTTGG TGGACTCGCT GTCAAGCCAC GCCCTCAATG CAGCAGGCGT GGCTGTTTCC 
TACCTCCTTG AGGGGGAGGA GCAAGCCAGA CTGCCAGCTC CGCCCCTGTC TATCTGTTCT 
GTAATAAAAG CGCAGAAGCC GGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332803.1
CDS	352..5940
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477128.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477128.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GGTGCTCTGG TTTTTCCTCC AGCCGATGAA 
GAGGCACCAG ACTATGGCTC TGGTGTCAGA CAGTCAGGCA CGGCCAAGAT TTCTTTTGAG 
GACCAGCAGT TTGAGAAGTT CCAGTCAGAG TCATGTCATT CTGGTCTTTC GCGTGCTGCC 
GTTTCTCAGG AAGAGTTGAG GATTGTGGAG GGTGAAGGAC AAAGTGCAGA GATGGGACTG 
TCAGCAGATG AGGTCAACAA TAATACATGT GCAGCTAACC CAGAGGAGCT GGACAGCCCT 
GTCAAAACAG GTCTTGATGG GATGCTGGAC GGCGCTCTGG ATCACTTGAG GATTGGTGAA 
GTCTTCACAT TCAGAGTTAC TGTTCTACAG GCGTCAAGCA TTTCCGCAGA GTACGCAGAC 
ATATTCTGCC AGTTCAATTT TATTCACAGG CACGATGAAG CATTTTCCAC CGAGCCGTTG 
AAGAACACTG GACGTGGTCC ACCACTTGGC TTCTATCATG TGCAGAATAT TGCAGTGGAA 
GTGACTAAGT CCTTTATTGA GTACATAAAA ACTCAGCCAA TCGTGTTTGA AGTCTTTGGA 
CACTACCAGA AGCAGCCTTT CCCTGCGCTC TGCAAGGATC TCATTAGCCC ATTGCGTCCA 
TGCAGACGAC AGTTCCCAAG GGTCATGCCT CTTTCCAAAC CAGCCGCAGA TAACGACACC 
CCTCCAGATC GGGAGAAGAA CCTGGAGCTG ATTCGTTACC TGCTGCCTGA GGTCTTCAAT 
GGTTCTTTGG TGGACTCGCT GTCAAGCCAC GCCCTCAATG CAGCAGGCGT GGCTGTTTCC 
TACCTCCTTG AGGGGGAGGA GCAAGCCAGA CTGCCAGCTC CGCCCCTGTC TATCTGTTCT 
GTAATAAAAG CGCAGAAGCC GGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477130.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332805.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X12, mRNA.

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CloneID	ODa143073
Clone ID Related Accession (Same CDS sequence)	XM_021477130.1
Accession Version	XM_021477130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGGTGC TCTGGTTTTT CCTCCAGCCG ATGAAGAGGC ACCAGACTAT 
GGCTCTGGTG TCAGACAGTC AGGCACGGCC AAGATTTCTT TTGAGGACCA GCAGTTTGAG 
AAGTTCCAGT CAGAGTCATG TCATTCTGGT CTTTCGCGTG CTGCCGTTTC TCAGGAAGAG 
TTGAGGATTG TGGAGGGTGA AGGACAAAGT GCAGAGATGG GACTGTCAGC AGATGAGGTC 
AACAATAATA CATGTGCAGC TAACCCAGAG GAGCTGGACA GCCCTGTCAA AACAGGTCTT 
GATGGGATGC TGGACGGCGC TCTGGATCAC TTGAGGATTG GTGAAGTCTT CACATTCAGA 
GTTACTGTTC TACAGGCGTC AAGCATTTCC GCAGAGTACG CAGACATATT CTGCCAGTTC 
AATTTTATTC ACAGGCACGA TGAAGCATTT TCCACCGAGC CGTTGAAGAA CACTGGACGT 
GGTCCACCAC TTGGCTTCTA TCATGTGCAG AATATTGCAG TGGAAGTGAC TAAGTCCTTT 
ATTGAGTACA TAAAAACTCA GCCAATCGTG TTTGAAGTCT TTGGACACTA CCAGAAGCAG 
CCTTTCCCTG CGCTCTGCAA GGATCTCATT AGCCCATTGC GTCCATGCAG ACGACAGTTC 
CCAAGGGTCA TGCCTCTTTC CAAACCAGCC GCAGATAACG ACACCCCTCC AGATCGGGAG 
AAGAACCTGG AGCTGATTCG TTACCTGCTG CCTGAGGTCT TCAATGGTTC TTTGGTGGAC 
TCGCTGTCAA GCCACGCCCT CAATGCAGCA GGCGTGGCTG TTTCCTACCT CCTTGAGGGG 
GAGGAGCAAG CCAGACTGCC AGCTCCGCCC CTGTCTATCT GTTCTGTAAT AAAAGCGCAG 
AAGCCGGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGACAGTT TCTTGATTCG GACATGCCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332805.1
CDS	352..5745
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477130.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477130.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGGTGC TCTGGTTTTT CCTCCAGCCG ATGAAGAGGC ACCAGACTAT 
GGCTCTGGTG TCAGACAGTC AGGCACGGCC AAGATTTCTT TTGAGGACCA GCAGTTTGAG 
AAGTTCCAGT CAGAGTCATG TCATTCTGGT CTTTCGCGTG CTGCCGTTTC TCAGGAAGAG 
TTGAGGATTG TGGAGGGTGA AGGACAAAGT GCAGAGATGG GACTGTCAGC AGATGAGGTC 
AACAATAATA CATGTGCAGC TAACCCAGAG GAGCTGGACA GCCCTGTCAA AACAGGTCTT 
GATGGGATGC TGGACGGCGC TCTGGATCAC TTGAGGATTG GTGAAGTCTT CACATTCAGA 
GTTACTGTTC TACAGGCGTC AAGCATTTCC GCAGAGTACG CAGACATATT CTGCCAGTTC 
AATTTTATTC ACAGGCACGA TGAAGCATTT TCCACCGAGC CGTTGAAGAA CACTGGACGT 
GGTCCACCAC TTGGCTTCTA TCATGTGCAG AATATTGCAG TGGAAGTGAC TAAGTCCTTT 
ATTGAGTACA TAAAAACTCA GCCAATCGTG TTTGAAGTCT TTGGACACTA CCAGAAGCAG 
CCTTTCCCTG CGCTCTGCAA GGATCTCATT AGCCCATTGC GTCCATGCAG ACGACAGTTC 
CCAAGGGTCA TGCCTCTTTC CAAACCAGCC GCAGATAACG ACACCCCTCC AGATCGGGAG 
AAGAACCTGG AGCTGATTCG TTACCTGCTG CCTGAGGTCT TCAATGGTTC TTTGGTGGAC 
TCGCTGTCAA GCCACGCCCT CAATGCAGCA GGCGTGGCTG TTTCCTACCT CCTTGAGGGG 
GAGGAGCAAG CCAGACTGCC AGCTCCGCCC CTGTCTATCT GTTCTGTAAT AAAAGCGCAG 
AAGCCGGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGACAGTT TCTTGATTCG GACATGCCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477131.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332806.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X13, mRNA.

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CloneID	ODa143074
Clone ID Related Accession (Same CDS sequence)	XM_021477131.1
Accession Version	XM_021477131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGCCG CAGATAACGA CACCCCTCCA GATCGGGAGA AGAACCTGGA GCTGATTCGT 
TACCTGCTGC CTGAGGTCTT CAATGGTTCT TTGGTGGACT CGCTGTCAAG CCACGCCCTC 
AATGCAGCAG GCGTGGCTGT TTCCTACCTC CTTGAGGGGG AGGAGCAAGC CAGACTGCCA 
GCTCCGCCCC TGTCTATCTG TTCTGTAATA AAAGCGCAGA AGCCGGTCCC TGCTACCAAA 
CTGACAGCCC TCACTCGACC CACAGCAGGA CCATGCCACT GTAAATATGA CCTGATGGTC 
TTCTTTGAGA TCTGCGAGCT GGAGGCTAAT GGAGACTATA TCCCAGCCGT AGTGGACCAC 
CGAGTAGGCA TGCCTTGCCA TGGCACATTC ATGTTACATC AGGGCATCCA AAGGAGAGTT 
ACAGTCACCA TGGTACATGA GACAGGAAGA GACATTGACT GGAAGGAAGT GCGAGAGCTG 
GTTGTAGGAC GTATCCGGAA CACGCCTGAA GCAGATGAGG CAATCATCGA CCCCAATATT 
CTCTCTCTGA ACATACTGTC TGCAGGATAC ATTCGACCCG CGCATGATGA CAGACAGTTT 
CTTGATTCGG ACATGCCTAG GACATTCTAC CGGTTTGAGG CTGCTTGGGA TTCATCCATG 
CACAACTCTT TGCTGCTAAA CCGTGTGACT CCTTACGGAG AGAAAATCTA CATGACTCTG 
TCTGCTTACC TGGAGATGGA GAACTGCACA CAACCCACAG TGATTACCAA AGACTTATGC 
ATGGTGTTCC ACTCAAGAGA CGCCAAACTT CCAGCATCTC GCTCCATCAG AAACCTCTTC 
AGCAGTGGCA CCTTAAGGCC GTCTGAAGGA AACCGTGTAA CCGGTGTGTA TGAGCTCAGT 
TTATGCCACC TGGCTGATGC AGGGAGCCCA GGAATGCAAA GGCGGCGCAG GAGGGTGCTG 
GACACGTCGG TGGCTTATGT GCGAGGGGAG GAGAACCTGG CTGGCTGGCG GCCCCGCAGT 
GACAGCCTCA TCCTGGACCA TCAGTGGGAG CTGGAGAAAT TGAGCCTCCT GCAGGAGGTG 
GAGAAAACCA GACACTACCT CCTCCTTAGG GAGAAGCTGG AGTCCACCCT GCTGCTGGGC 
CAGGAGTCGC TGCTGTCCTG CGGGAGTGAG GATCTAACCG AGTCCTCTTC CCCCTCCACT 
CACCTCGGCC TGGGCCACAA CCCCGGCCCC GGCCCCGACA CACCCAATGA GAGGCAGAGG 
GAGCTGGCTG CTAAGTGTCT GCGTTTGCTT AACCACACAT TTAACAGGGA CTACAGCCAT 
GTGTGTGTTA GCGCCAGTGA GAGCAAGTTG AGTGAGATGT CAGTGACTCT CCTGAAAGAC 
TCTGCTTCAG TGTGTGCCGA AAGCACCCTC ACACCCTCCT CCACATGTCC TTCACTTGTG 
GAGAGTCGAT ACATTCACAA CACTGATCTC AGACCCCCAA CACCCCGATC TCGTGCTGTG 
AGCCCTAATC CTGACTCTGA AACAGATGGT AAGAAGATTC AAGAGCCCAG ATCAAGAACC 
CGCACTTTTG TGCCAGACAT TCAGGAAATC CGTGTCAGCC CAATTGTGTC AAAGAAAGGC 
TATTTGCACT TTCTGGAGCC CCACAGTAAT GGCTGGGTTA AGAGATATGT GGTGGTTCGT 
CGGCCTTATG TCTACATCTA CAACTCAGAC AGAGACACAG TGGAAAGGGC CATTCTTAAC 
CTGTCCTCAG CGCAAGTGGA GTATAGCGAA GACCAGCAGG CCATGCTAAA GACCCCCTAC 
ACATTTGCGG TATGCACAGA GCATCGTGGG ATACTCCTTC AAGCCAGTAA TGACAAGGAG 
ATGCATGACT GGCTGTATGC CTTTAACCCT CTGCTTGCTG GATCTATCAG GTCTAAACTG 
TCCAGAAGGA GAGCTGGGCA GATGAGGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332806.1
CDS	351..5723
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPAADNDTPPDREKNLELIRYLLPEVFNGSLVDSLSSHALNAAGVAVSYLLEGEEQARLPAPPLSICSVIKAQKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477131.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477131.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGCCG CAGATAACGA CACCCCTCCA GATCGGGAGA AGAACCTGGA GCTGATTCGT 
TACCTGCTGC CTGAGGTCTT CAATGGTTCT TTGGTGGACT CGCTGTCAAG CCACGCCCTC 
AATGCAGCAG GCGTGGCTGT TTCCTACCTC CTTGAGGGGG AGGAGCAAGC CAGACTGCCA 
GCTCCGCCCC TGTCTATCTG TTCTGTAATA AAAGCGCAGA AGCCGGTCCC TGCTACCAAA 
CTGACAGCCC TCACTCGACC CACAGCAGGA CCATGCCACT GTAAATATGA CCTGATGGTC 
TTCTTTGAGA TCTGCGAGCT GGAGGCTAAT GGAGACTATA TCCCAGCCGT AGTGGACCAC 
CGAGTAGGCA TGCCTTGCCA TGGCACATTC ATGTTACATC AGGGCATCCA AAGGAGAGTT 
ACAGTCACCA TGGTACATGA GACAGGAAGA GACATTGACT GGAAGGAAGT GCGAGAGCTG 
GTTGTAGGAC GTATCCGGAA CACGCCTGAA GCAGATGAGG CAATCATCGA CCCCAATATT 
CTCTCTCTGA ACATACTGTC TGCAGGATAC ATTCGACCCG CGCATGATGA CAGACAGTTT 
CTTGATTCGG ACATGCCTAG GACATTCTAC CGGTTTGAGG CTGCTTGGGA TTCATCCATG 
CACAACTCTT TGCTGCTAAA CCGTGTGACT CCTTACGGAG AGAAAATCTA CATGACTCTG 
TCTGCTTACC TGGAGATGGA GAACTGCACA CAACCCACAG TGATTACCAA AGACTTATGC 
ATGGTGTTCC ACTCAAGAGA CGCCAAACTT CCAGCATCTC GCTCCATCAG AAACCTCTTC 
AGCAGTGGCA CCTTAAGGCC GTCTGAAGGA AACCGTGTAA CCGGTGTGTA TGAGCTCAGT 
TTATGCCACC TGGCTGATGC AGGGAGCCCA GGAATGCAAA GGCGGCGCAG GAGGGTGCTG 
GACACGTCGG TGGCTTATGT GCGAGGGGAG GAGAACCTGG CTGGCTGGCG GCCCCGCAGT 
GACAGCCTCA TCCTGGACCA TCAGTGGGAG CTGGAGAAAT TGAGCCTCCT GCAGGAGGTG 
GAGAAAACCA GACACTACCT CCTCCTTAGG GAGAAGCTGG AGTCCACCCT GCTGCTGGGC 
CAGGAGTCGC TGCTGTCCTG CGGGAGTGAG GATCTAACCG AGTCCTCTTC CCCCTCCACT 
CACCTCGGCC TGGGCCACAA CCCCGGCCCC GGCCCCGACA CACCCAATGA GAGGCAGAGG 
GAGCTGGCTG CTAAGTGTCT GCGTTTGCTT AACCACACAT TTAACAGGGA CTACAGCCAT 
GTGTGTGTTA GCGCCAGTGA GAGCAAGTTG AGTGAGATGT CAGTGACTCT CCTGAAAGAC 
TCTGCTTCAG TGTGTGCCGA AAGCACCCTC ACACCCTCCT CCACATGTCC TTCACTTGTG 
GAGAGTCGAT ACATTCACAA CACTGATCTC AGACCCCCAA CACCCCGATC TCGTGCTGTG 
AGCCCTAATC CTGACTCTGA AACAGATGGT AAGAAGATTC AAGAGCCCAG ATCAAGAACC 
CGCACTTTTG TGCCAGACAT TCAGGAAATC CGTGTCAGCC CAATTGTGTC AAAGAAAGGC 
TATTTGCACT TTCTGGAGCC CCACAGTAAT GGCTGGGTTA AGAGATATGT GGTGGTTCGT 
CGGCCTTATG TCTACATCTA CAACTCAGAC AGAGACACAG TGGAAAGGGC CATTCTTAAC 
CTGTCCTCAG CGCAAGTGGA GTATAGCGAA GACCAGCAGG CCATGCTAAA GACCCCCTAC 
ACATTTGCGG TATGCACAGA GCATCGTGGG ATACTCCTTC AAGCCAGTAA TGACAAGGAG 
ATGCATGACT GGCTGTATGC CTTTAACCCT CTGCTTGCTG GATCTATCAG GTCTAAACTG 
TCCAGAAGGA GAGCTGGGCA GATGAGGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477132.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332807.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.

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CloneID	ODa143075
Clone ID Related Accession (Same CDS sequence)	XM_021477132.1
Accession Version	XM_021477132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGACAGTT TCTTGATTCG GACATGCCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332807.1
CDS	351..5504
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477132.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477132.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGACAGTT TCTTGATTCG GACATGCCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_021477129.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332804.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.

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CloneID	ODa49317
Clone ID Related Accession (Same CDS sequence)	XM_021477129.1
Accession Version	XM_021477129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGCAT TTCCTTGGGG ACTGATTCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332804.1
CDS	352..5805
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDSISLGTDSRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_021477129.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477129.1

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGCAT TTCCTTGGGG ACTGATTCTA GGACATTCTA CCGGTTTGAG 
GCTGCTTGGG ATTCATCCAT GCACAACTCT TTGCTGCTAA ACCGTGTGAC TCCTTACGGA 
GAGAAAATCT ACATGACTCT GTCTGCTTAC CTGGAGATGG AGAACTGCAC ACAACCCACA 
GTGATTACCA AAGACTTATG CATGGTGTTC CACTCAAGAG ACGCCAAACT TCCAGCATCT 
CGCTCCATCA GAAACCTCTT CAGCAGTGGC ACCTTAAGGC CGTCTGAAGG AAACCGTGTA 
ACCGGTGTGT ATGAGCTCAG TTTATGCCAC CTGGCTGATG CAGGGAGCCC AGGAATGCAA 
AGGCGGCGCA GGAGGGTGCT GGACACGTCG GTGGCTTATG TGCGAGGGGA GGAGAACCTG 
GCTGGCTGGC GGCCCCGCAG TGACAGCCTC ATCCTGGACC ATCAGTGGGA GCTGGAGAAA 
TTGAGCCTCC TGCAGGAGGT GGAGAAAACC AGACACTACC TCCTCCTTAG GGAGAAGCTG 
GAGTCCACCC TGCTGCTGGG CCAGGAGTCG CTGCTGTCCT GCGGGAGTGA GGATCTAACC 
GAGTCCTCTT CCCCCTCCAC TCACCTCGGC CTGGGCCACA ACCCCGGCCC CGGCCCCGAC 
ACACCCAATG AGAGGCAGAG GGAGCTGGCT GCTAAGTGTC TGCGTTTGCT TAACCACACA 
TTTAACAGGG ACTACAGCCA TGTGTGTGTT AGCGCCAGTG AGAGCAAGTT GAGTGAGATG 
TCAGTGACTC TCCTGAAAGA CTCTGCTTCA GTGTGTGCCG AAAGCACCCT CACACCCTCC 
TCCACATGTC CTTCACTTGT GGAGAGTCGA TACATTCACA ACACTGATCT CAGACCCCCA 
ACACCCCGAT CTCGTGCTGT GAGCCCTAAT CCTGACTCTG AAACAGATGG TAAGAAGATT 
CAAGAGCCCA GATCAAGAAC CCGCACTTTT GTGCCAGACA TTCAGGAAAT CCGTGTCAGC 
CCAATTGTGT CAAAGAAAGG CTATTTGCAC TTTCTGGAGC CCCACAGTAA TGGCTGGGTT 
AAGAGATATG TGGTGGTTCG TCGGCCTTAT GTCTACATCT ACAACTCAGA CAGAGACACA 
GTGGAAAGGG CCATTCTTAA CCTGTCCTCA GCGCAAGTGG AGTATAGCGA AGACCAGCAG 
GCCATGCTAA AGACCCCCTA CACATTTGCG GTATGCACAG AGCATCGTGG GATACTCCTT 
CAAGCCAGTA ATGACAAGGA GATGCATGAC TGGCTGTATG CCTTTAACCC TCTGCTTGCT 
GGATCTATCA GGTCTAAACT GTCCAGAAGG AGAGCTGGGC AGATGAGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165945.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166002.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.

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CloneID	ODa11872
Clone ID Related Accession (Same CDS sequence)	XM_005165945.4 , XM_005165945.3
Accession Version	XM_005165945.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165945.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166002.1
CDS	352..5829
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165945.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165945.4

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165945.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166002.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.

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CloneID	ODa11872
Clone ID Related Accession (Same CDS sequence)	XM_005165945.4 , XM_005165945.3
Accession Version	XM_005165945.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166002.1
CDS	350..5827
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165945.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165945.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGAC AGTTTCTTGA TTCGGACATG 
CCTAGGACAT TCTACCGGTT TGAGGCTGCT TGGGATTCAT CCATGCACAA CTCTTTGCTG 
CTAAACCGTG TGACTCCTTA CGGAGAGAAA ATCTACATGA CTCTGTCTGC TTACCTGGAG 
ATGGAGAACT GCACACAACC CACAGTGATT ACCAAAGACT TATGCATGGT GTTCCACTCA 
AGAGACGCCA AACTTCCAGC ATCTCGCTCC ATCAGAAACC TCTTCAGCAG TGGCACCTTA 
AGGCCGTCTG AAGGAAACCG TGTAACCGGT GTGTATGAGC TCAGTTTATG CCACCTGGCT 
GATGCAGGGA GCCCAGGAAT GCAAAGGCGG CGCAGGAGGG TGCTGGACAC GTCGGTGGCT 
TATGTGCGAG GGGAGGAGAA CCTGGCTGGC TGGCGGCCCC GCAGTGACAG CCTCATCCTG 
GACCATCAGT GGGAGCTGGA GAAATTGAGC CTCCTGCAGG AGGTGGAGAA AACCAGACAC 
TACCTCCTCC TTAGGGAGAA GCTGGAGTCC ACCCTGCTGC TGGGCCAGGA GTCGCTGCTG 
TCCTGCGGGA GTGAGGATCT AACCGAGTCC TCTTCCCCCT CCACTCACCT CGGCCTGGGC 
CACAACCCCG GCCCCGGCCC CGACACACCC AATGAGAGGC AGAGGGAGCT GGCTGCTAAG 
TGTCTGCGTT TGCTTAACCA CACATTTAAC AGGGACTACA GCCATGTGTG TGTTAGCGCC 
AGTGAGAGCA AGTTGAGTGA GATGTCAGTG ACTCTCCTGA AAGACTCTGC TTCAGTGTGT 
GCCGAAAGCA CCCTCACACC CTCCTCCACA TGTCCTTCAC TTGTGGAGAG TCGATACATT 
CACAACACTG ATCTCAGACC CCCAACACCC CGATCTCGTG CTGTGAGCCC TAATCCTGAC 
TCTGAAACAG ATGGTAAGAA GATTCAAGAG CCCAGATCAA GAACCCGCAC TTTTGTGCCA 
GACATTCAGG AAATCCGTGT CAGCCCAATT GTGTCAAAGA AAGGCTATTT GCACTTTCTG 
GAGCCCCACA GTAATGGCTG GGTTAAGAGA TATGTGGTGG TTCGTCGGCC TTATGTCTAC 
ATCTACAACT CAGACAGAGA CACAGTGGAA AGGGCCATTC TTAACCTGTC CTCAGCGCAA 
GTGGAGTATA GCGAAGACCA GCAGGCCATG CTAAAGACCC CCTACACATT TGCGGTATGC 
ACAGAGCATC GTGGGATACT CCTTCAAGCC AGTAATGACA AGGAGATGCA TGACTGGCTG 
TATGCCTTTA ACCCTCTGCT TGCTGGATCT ATCAGGTCTA AACTGTCCAG AAGGAGAGCT 
GGGCAGATGA GGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165946.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166003.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.

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CloneID	ODa11873
Clone ID Related Accession (Same CDS sequence)	XM_005165946.3
Accession Version	XM_005165946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGCA TTTCCTTGGG GACTGATTCT 
AGGACATTCT ACCGGTTTGA GGCTGCTTGG GATTCATCCA TGCACAACTC TTTGCTGCTA 
AACCGTGTGA CTCCTTACGG AGAGAAAATC TACATGACTC TGTCTGCTTA CCTGGAGATG 
GAGAACTGCA CACAACCCAC AGTGATTACC AAAGACTTAT GCATGGTGTT CCACTCAAGA 
GACGCCAAAC TTCCAGCATC TCGCTCCATC AGAAACCTCT TCAGCAGTGG CACCTTAAGG 
CCGTCTGAAG GAAACCGTGT AACCGGTGTG TATGAGCTCA GTTTATGCCA CCTGGCTGAT 
GCAGGGAGCC CAGGAATGCA AAGGCGGCGC AGGAGGGTGC TGGACACGTC GGTGGCTTAT 
GTGCGAGGGG AGGAGAACCT GGCTGGCTGG CGGCCCCGCA GTGACAGCCT CATCCTGGAC 
CATCAGTGGG AGCTGGAGAA ATTGAGCCTC CTGCAGGAGG TGGAGAAAAC CAGACACTAC 
CTCCTCCTTA GGGAGAAGCT GGAGTCCACC CTGCTGCTGG GCCAGGAGTC GCTGCTGTCC 
TGCGGGAGTG AGGATCTAAC CGAGTCCTCT TCCCCCTCCA CTCACCTCGG CCTGGGCCAC 
AACCCCGGCC CCGGCCCCGA CACACCCAAT GAGAGGCAGA GGGAGCTGGC TGCTAAGTGT 
CTGCGTTTGC TTAACCACAC ATTTAACAGG GACTACAGCC ATGTGTGTGT TAGCGCCAGT 
GAGAGCAAGT TGAGTGAGAT GTCAGTGACT CTCCTGAAAG ACTCTGCTTC AGTGTGTGCC 
GAAAGCACCC TCACACCCTC CTCCACATGT CCTTCACTTG TGGAGAGTCG ATACATTCAC 
AACACTGATC TCAGACCCCC AACACCCCGA TCTCGTGCTG TGAGCCCTAA TCCTGACTCT 
GAAACAGATG GTAAGAAGAT TCAAGAGCCC AGATCAAGAA CCCGCACTTT TGTGCCAGAC 
ATTCAGGAAA TCCGTGTCAG CCCAATTGTG TCAAAGAAAG GCTATTTGCA CTTTCTGGAG 
CCCCACAGTA ATGGCTGGGT TAAGAGATAT GTGGTGGTTC GTCGGCCTTA TGTCTACATC 
TACAACTCAG ACAGAGACAC AGTGGAAAGG GCCATTCTTA ACCTGTCCTC AGCGCAAGTG 
GAGTATAGCG AAGACCAGCA GGCCATGCTA AAGACCCCCT ACACATTTGC GGTATGCACA 
GAGCATCGTG GGATACTCCT TCAAGCCAGT AATGACAAGG AGATGCATGA CTGGCTGTAT 
GCCTTTAACC CTCTGCTTGC TGGATCTATC AGGTCTAAAC TGTCCAGAAG GAGAGCTGGG 
CAGATGAGGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166003.1
CDS	350..5824
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDSISLGTDSRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165946.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165946.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGCA TTTCCTTGGG GACTGATTCT 
AGGACATTCT ACCGGTTTGA GGCTGCTTGG GATTCATCCA TGCACAACTC TTTGCTGCTA 
AACCGTGTGA CTCCTTACGG AGAGAAAATC TACATGACTC TGTCTGCTTA CCTGGAGATG 
GAGAACTGCA CACAACCCAC AGTGATTACC AAAGACTTAT GCATGGTGTT CCACTCAAGA 
GACGCCAAAC TTCCAGCATC TCGCTCCATC AGAAACCTCT TCAGCAGTGG CACCTTAAGG 
CCGTCTGAAG GAAACCGTGT AACCGGTGTG TATGAGCTCA GTTTATGCCA CCTGGCTGAT 
GCAGGGAGCC CAGGAATGCA AAGGCGGCGC AGGAGGGTGC TGGACACGTC GGTGGCTTAT 
GTGCGAGGGG AGGAGAACCT GGCTGGCTGG CGGCCCCGCA GTGACAGCCT CATCCTGGAC 
CATCAGTGGG AGCTGGAGAA ATTGAGCCTC CTGCAGGAGG TGGAGAAAAC CAGACACTAC 
CTCCTCCTTA GGGAGAAGCT GGAGTCCACC CTGCTGCTGG GCCAGGAGTC GCTGCTGTCC 
TGCGGGAGTG AGGATCTAAC CGAGTCCTCT TCCCCCTCCA CTCACCTCGG CCTGGGCCAC 
AACCCCGGCC CCGGCCCCGA CACACCCAAT GAGAGGCAGA GGGAGCTGGC TGCTAAGTGT 
CTGCGTTTGC TTAACCACAC ATTTAACAGG GACTACAGCC ATGTGTGTGT TAGCGCCAGT 
GAGAGCAAGT TGAGTGAGAT GTCAGTGACT CTCCTGAAAG ACTCTGCTTC AGTGTGTGCC 
GAAAGCACCC TCACACCCTC CTCCACATGT CCTTCACTTG TGGAGAGTCG ATACATTCAC 
AACACTGATC TCAGACCCCC AACACCCCGA TCTCGTGCTG TGAGCCCTAA TCCTGACTCT 
GAAACAGATG GTAAGAAGAT TCAAGAGCCC AGATCAAGAA CCCGCACTTT TGTGCCAGAC 
ATTCAGGAAA TCCGTGTCAG CCCAATTGTG TCAAAGAAAG GCTATTTGCA CTTTCTGGAG 
CCCCACAGTA ATGGCTGGGT TAAGAGATAT GTGGTGGTTC GTCGGCCTTA TGTCTACATC 
TACAACTCAG ACAGAGACAC AGTGGAAAGG GCCATTCTTA ACCTGTCCTC AGCGCAAGTG 
GAGTATAGCG AAGACCAGCA GGCCATGCTA AAGACCCCCT ACACATTTGC GGTATGCACA 
GAGCATCGTG GGATACTCCT TCAAGCCAGT AATGACAAGG AGATGCATGA CTGGCTGTAT 
GCCTTTAACC CTCTGCTTGC TGGATCTATC AGGTCTAAAC TGTCCAGAAG GAGAGCTGGG 
CAGATGAGGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165947.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166004.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.

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CloneID	ODa11874
Clone ID Related Accession (Same CDS sequence)	XM_005165947.3
Accession Version	XM_005165947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGACA GTTTCTTGAT TCGGACATGC CTAGGACATT CTACCGGTTT 
GAGGCTGCTT GGGATTCATC CATGCACAAC TCTTTGCTGC TAAACCGTGT GACTCCTTAC 
GGAGAGAAAA TCTACATGAC TCTGTCTGCT TACCTGGAGA TGGAGAACTG CACACAACCC 
ACAGTGATTA CCAAAGACTT ATGCATGGTG TTCCACTCAA GAGACGCCAA ACTTCCAGCA 
TCTCGCTCCA TCAGAAACCT CTTCAGCAGT GGCACCTTAA GGCCGTCTGA AGGAAACCGT 
GTAACCGGTG TGTATGAGCT CAGTTTATGC CACCTGGCTG ATGCAGGGAG CCCAGGAATG 
CAAAGGCGGC GCAGGAGGGT GCTGGACACG TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC 
CTGGCTGGCT GGCGGCCCCG CAGTGACAGC CTCATCCTGG ACCATCAGTG GGAGCTGGAG 
AAATTGAGCC TCCTGCAGGA GGTGGAGAAA ACCAGACACT ACCTCCTCCT TAGGGAGAAG 
CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG TCGCTGCTGT CCTGCGGGAG TGAGGATCTA 
ACCGAGTCCT CTTCCCCCTC CACTCACCTC GGCCTGGGCC ACAACCCCGG CCCCGGCCCC 
GACACACCCA ATGAGAGGCA GAGGGAGCTG GCTGCTAAGT GTCTGCGTTT GCTTAACCAC 
ACATTTAACA GGGACTACAG CCATGTGTGT GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG 
ATGTCAGTGA CTCTCCTGAA AGACTCTGCT TCAGTGTGTG CCGAAAGCAC CCTCACACCC 
TCCTCCACAT GTCCTTCACT TGTGGAGAGT CGATACATTC ACAACACTGA TCTCAGACCC 
CCAACACCCC GATCTCGTGC TGTGAGCCCT AATCCTGACT CTGAAACAGA TGGTAAGAAG 
ATTCAAGAGC CCAGATCAAG AACCCGCACT TTTGTGCCAG ACATTCAGGA AATCCGTGTC 
AGCCCAATTG TGTCAAAGAA AGGCTATTTG CACTTTCTGG AGCCCCACAG TAATGGCTGG 
GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT TATGTCTACA TCTACAACTC AGACAGAGAC 
ACAGTGGAAA GGGCCATTCT TAACCTGTCC TCAGCGCAAG TGGAGTATAG CGAAGACCAG 
CAGGCCATGC TAAAGACCCC CTACACATTT GCGGTATGCA CAGAGCATCG TGGGATACTC 
CTTCAAGCCA GTAATGACAA GGAGATGCAT GACTGGCTGT ATGCCTTTAA CCCTCTGCTT 
GCTGGATCTA TCAGGTCTAA ACTGTCCAGA AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166004.1
CDS	350..5806
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165947.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165947.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGACA GTTTCTTGAT TCGGACATGC CTAGGACATT CTACCGGTTT 
GAGGCTGCTT GGGATTCATC CATGCACAAC TCTTTGCTGC TAAACCGTGT GACTCCTTAC 
GGAGAGAAAA TCTACATGAC TCTGTCTGCT TACCTGGAGA TGGAGAACTG CACACAACCC 
ACAGTGATTA CCAAAGACTT ATGCATGGTG TTCCACTCAA GAGACGCCAA ACTTCCAGCA 
TCTCGCTCCA TCAGAAACCT CTTCAGCAGT GGCACCTTAA GGCCGTCTGA AGGAAACCGT 
GTAACCGGTG TGTATGAGCT CAGTTTATGC CACCTGGCTG ATGCAGGGAG CCCAGGAATG 
CAAAGGCGGC GCAGGAGGGT GCTGGACACG TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC 
CTGGCTGGCT GGCGGCCCCG CAGTGACAGC CTCATCCTGG ACCATCAGTG GGAGCTGGAG 
AAATTGAGCC TCCTGCAGGA GGTGGAGAAA ACCAGACACT ACCTCCTCCT TAGGGAGAAG 
CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG TCGCTGCTGT CCTGCGGGAG TGAGGATCTA 
ACCGAGTCCT CTTCCCCCTC CACTCACCTC GGCCTGGGCC ACAACCCCGG CCCCGGCCCC 
GACACACCCA ATGAGAGGCA GAGGGAGCTG GCTGCTAAGT GTCTGCGTTT GCTTAACCAC 
ACATTTAACA GGGACTACAG CCATGTGTGT GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG 
ATGTCAGTGA CTCTCCTGAA AGACTCTGCT TCAGTGTGTG CCGAAAGCAC CCTCACACCC 
TCCTCCACAT GTCCTTCACT TGTGGAGAGT CGATACATTC ACAACACTGA TCTCAGACCC 
CCAACACCCC GATCTCGTGC TGTGAGCCCT AATCCTGACT CTGAAACAGA TGGTAAGAAG 
ATTCAAGAGC CCAGATCAAG AACCCGCACT TTTGTGCCAG ACATTCAGGA AATCCGTGTC 
AGCCCAATTG TGTCAAAGAA AGGCTATTTG CACTTTCTGG AGCCCCACAG TAATGGCTGG 
GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT TATGTCTACA TCTACAACTC AGACAGAGAC 
ACAGTGGAAA GGGCCATTCT TAACCTGTCC TCAGCGCAAG TGGAGTATAG CGAAGACCAG 
CAGGCCATGC TAAAGACCCC CTACACATTT GCGGTATGCA CAGAGCATCG TGGGATACTC 
CTTCAAGCCA GTAATGACAA GGAGATGCAT GACTGGCTGT ATGCCTTTAA CCCTCTGCTT 
GCTGGATCTA TCAGGTCTAA ACTGTCCAGA AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165948.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166005.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.

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CloneID	ODa11875
Clone ID Related Accession (Same CDS sequence)	XM_005165948.3
Accession Version	XM_005165948.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGTGA GCAAGCCAGC GACCCCAACT CCTCCCCCAC CCCCTCTGAA 
CCCACCTCCA CCTCTGAACT CACCGAGCTC GTCCAGTCAT GGCCAGACAA GAAGGTGGGG 
ACAGAGCTGG ATGACGATGG TTTCTTATCA GACGACCCGT GCTCGGATTT AGAGGAGGAG 
GGGGAGGATG ATGATGATGA TGAGGTTGAT GATGACGAAG AGCTCTGCAG AAGCTCCGGC 
AAAGCTGAGG ACCCGTTTTA TGATCGTTCA CCATTGTTCA GTGTAGTAGG AAGGGCTTTT 
GTCTATTTGA GTAATCTGCT GTATCCTGTG CCTCTGGTCC ATCGGGTTGC CATCGTCAAT 
GAGAAGGGAG AAGTCAAAGG TTTCCTCAGA GTAGCTGTAC AGGCAATATC AGGTGCTCTG 
GTTTTTCCTC CAGCCGATGA AGAGGCACCA GACTATGGCT CTGGTGTCAG ACAGTCAGGC 
ACGGCCAAGA TTTCTTTTGA GGACCAGCAG TTTGAGAAGT TCCAGTCAGA GTCATGTCAT 
TCTGGTCTTT CGCGTGCTGC CGTTTCTCAG GAAGAGTTGA GGATTGTGGA GGGTGAAGGA 
CAAAGTGCAG AGATGGGACT GTCAGCAGAT GAGGTCAACA ATAATACATG TGCAGCTAAC 
CCAGAGGAGC TGGACAGCCC TGTCAAAACA GGTCTTGATG GGATGCTGGA CGGCGCTCTG 
GATCACTTGA GGATTGGTGA AGTCTTCACA TTCAGAGTTA CTGTTCTACA GGCGTCAAGC 
ATTTCCGCAG AGTACGCAGA CATATTCTGC CAGTTCAATT TTATTCACAG GCACGATGAA 
GCATTTTCCA CCGAGCCGTT GAAGAACACT GGACGTGGTC CACCACTTGG CTTCTATCAT 
GTGCAGAATA TTGCAGTGGA AGTGACTAAG TCCTTTATTG AGTACATAAA AACTCAGCCA 
ATCGTGTTTG AAGTCTTTGG ACACTACCAG AAGCAGCCTT TCCCTGCGCT CTGCAAGGAT 
CTCATTAGCC CATTGCGTCC ATGCAGACGA CAGTTCCCAA GGGTCATGCC TCTTTCCAAA 
CCAGTCCCTG CTACCAAACT GACAGCCCTC ACTCGACCCA CAGCAGGACC ATGCCACTGT 
AAATATGACC TGATGGTCTT CTTTGAGATC TGCGAGCTGG AGGCTAATGG AGACTATATC 
CCAGCCGTAG TGGACCACCG AGTAGGCATG CCTTGCCATG GCACATTCAT GTTACATCAG 
GGCATCCAAA GGAGAGTTAC AGTCACCATG GTACATGAGA CAGGAAGAGA CATTGACTGG 
AAGGAAGTGC GAGAGCTGGT TGTAGGACGT ATCCGGAACA CGCCTGAAGC AGATGAGGCA 
ATCATCGACC CCAATATTCT CTCTCTGAAC ATACTGTCTG CAGGATACAT TCGACCCGCG 
CATGATGACA GACAGTTTCT TGATTCGGAC ATGCCTAGGA CATTCTACCG GTTTGAGGCT 
GCTTGGGATT CATCCATGCA CAACTCTTTG CTGCTAAACC GTGTGACTCC TTACGGAGAG 
AAAATCTACA TGACTCTGTC TGCTTACCTG GAGATGGAGA ACTGCACACA ACCCACAGTG 
ATTACCAAAG ACTTATGCAT GGTGTTCCAC TCAAGAGACG CCAAACTTCC AGCATCTCGC 
TCCATCAGAA ACCTCTTCAG CAGTGGCACC TTAAGGCCGT CTGAAGGAAA CCGTGTAACC 
GGTGTGTATG AGCTCAGTTT ATGCCACCTG GCTGATGCAG GGAGCCCAGG AATGCAAAGG 
CGGCGCAGGA GGGTGCTGGA CACGTCGGTG GCTTATGTGC GAGGGGAGGA GAACCTGGCT 
GGCTGGCGGC CCCGCAGTGA CAGCCTCATC CTGGACCATC AGTGGGAGCT GGAGAAATTG 
AGCCTCCTGC AGGAGGTGGA GAAAACCAGA CACTACCTCC TCCTTAGGGA GAAGCTGGAG 
TCCACCCTGC TGCTGGGCCA GGAGTCGCTG CTGTCCTGCG GGAGTGAGGA TCTAACCGAG 
TCCTCTTCCC CCTCCACTCA CCTCGGCCTG GGCCACAACC CCGGCCCCGG CCCCGACACA 
CCCAATGAGA GGCAGAGGGA GCTGGCTGCT AAGTGTCTGC GTTTGCTTAA CCACACATTT 
AACAGGGACT ACAGCCATGT GTGTGTTAGC GCCAGTGAGA GCAAGTTGAG TGAGATGTCA 
GTGACTCTCC TGAAAGACTC TGCTTCAGTG TGTGCCGAAA GCACCCTCAC ACCCTCCTCC 
ACATGTCCTT CACTTGTGGA GAGTCGATAC ATTCACAACA CTGATCTCAG ACCCCCAACA 
CCCCGATCTC GTGCTGTGAG CCCTAATCCT GACTCTGAAA CAGATGGTAA GAAGATTCAA 
GAGCCCAGAT CAAGAACCCG CACTTTTGTG CCAGACATTC AGGAAATCCG TGTCAGCCCA 
ATTGTGTCAA AGAAAGGCTA TTTGCACTTT CTGGAGCCCC ACAGTAATGG CTGGGTTAAG 
AGATATGTGG TGGTTCGTCG GCCTTATGTC TACATCTACA ACTCAGACAG AGACACAGTG 
GAAAGGGCCA TTCTTAACCT GTCCTCAGCG CAAGTGGAGT ATAGCGAAGA CCAGCAGGCC 
ATGCTAAAGA CCCCCTACAC ATTTGCGGTA TGCACAGAGC ATCGTGGGAT ACTCCTTCAA 
GCCAGTAATG ACAAGGAGAT GCATGACTGG CTGTATGCCT TTAACCCTCT GCTTGCTGGA 
TCTATCAGGT CTAAACTGTC CAGAAGGAGA GCTGGGCAGA TGAGGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166005.1
CDS	350..5800
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165948.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165948.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGTGA GCAAGCCAGC GACCCCAACT CCTCCCCCAC CCCCTCTGAA 
CCCACCTCCA CCTCTGAACT CACCGAGCTC GTCCAGTCAT GGCCAGACAA GAAGGTGGGG 
ACAGAGCTGG ATGACGATGG TTTCTTATCA GACGACCCGT GCTCGGATTT AGAGGAGGAG 
GGGGAGGATG ATGATGATGA TGAGGTTGAT GATGACGAAG AGCTCTGCAG AAGCTCCGGC 
AAAGCTGAGG ACCCGTTTTA TGATCGTTCA CCATTGTTCA GTGTAGTAGG AAGGGCTTTT 
GTCTATTTGA GTAATCTGCT GTATCCTGTG CCTCTGGTCC ATCGGGTTGC CATCGTCAAT 
GAGAAGGGAG AAGTCAAAGG TTTCCTCAGA GTAGCTGTAC AGGCAATATC AGGTGCTCTG 
GTTTTTCCTC CAGCCGATGA AGAGGCACCA GACTATGGCT CTGGTGTCAG ACAGTCAGGC 
ACGGCCAAGA TTTCTTTTGA GGACCAGCAG TTTGAGAAGT TCCAGTCAGA GTCATGTCAT 
TCTGGTCTTT CGCGTGCTGC CGTTTCTCAG GAAGAGTTGA GGATTGTGGA GGGTGAAGGA 
CAAAGTGCAG AGATGGGACT GTCAGCAGAT GAGGTCAACA ATAATACATG TGCAGCTAAC 
CCAGAGGAGC TGGACAGCCC TGTCAAAACA GGTCTTGATG GGATGCTGGA CGGCGCTCTG 
GATCACTTGA GGATTGGTGA AGTCTTCACA TTCAGAGTTA CTGTTCTACA GGCGTCAAGC 
ATTTCCGCAG AGTACGCAGA CATATTCTGC CAGTTCAATT TTATTCACAG GCACGATGAA 
GCATTTTCCA CCGAGCCGTT GAAGAACACT GGACGTGGTC CACCACTTGG CTTCTATCAT 
GTGCAGAATA TTGCAGTGGA AGTGACTAAG TCCTTTATTG AGTACATAAA AACTCAGCCA 
ATCGTGTTTG AAGTCTTTGG ACACTACCAG AAGCAGCCTT TCCCTGCGCT CTGCAAGGAT 
CTCATTAGCC CATTGCGTCC ATGCAGACGA CAGTTCCCAA GGGTCATGCC TCTTTCCAAA 
CCAGTCCCTG CTACCAAACT GACAGCCCTC ACTCGACCCA CAGCAGGACC ATGCCACTGT 
AAATATGACC TGATGGTCTT CTTTGAGATC TGCGAGCTGG AGGCTAATGG AGACTATATC 
CCAGCCGTAG TGGACCACCG AGTAGGCATG CCTTGCCATG GCACATTCAT GTTACATCAG 
GGCATCCAAA GGAGAGTTAC AGTCACCATG GTACATGAGA CAGGAAGAGA CATTGACTGG 
AAGGAAGTGC GAGAGCTGGT TGTAGGACGT ATCCGGAACA CGCCTGAAGC AGATGAGGCA 
ATCATCGACC CCAATATTCT CTCTCTGAAC ATACTGTCTG CAGGATACAT TCGACCCGCG 
CATGATGACA GACAGTTTCT TGATTCGGAC ATGCCTAGGA CATTCTACCG GTTTGAGGCT 
GCTTGGGATT CATCCATGCA CAACTCTTTG CTGCTAAACC GTGTGACTCC TTACGGAGAG 
AAAATCTACA TGACTCTGTC TGCTTACCTG GAGATGGAGA ACTGCACACA ACCCACAGTG 
ATTACCAAAG ACTTATGCAT GGTGTTCCAC TCAAGAGACG CCAAACTTCC AGCATCTCGC 
TCCATCAGAA ACCTCTTCAG CAGTGGCACC TTAAGGCCGT CTGAAGGAAA CCGTGTAACC 
GGTGTGTATG AGCTCAGTTT ATGCCACCTG GCTGATGCAG GGAGCCCAGG AATGCAAAGG 
CGGCGCAGGA GGGTGCTGGA CACGTCGGTG GCTTATGTGC GAGGGGAGGA GAACCTGGCT 
GGCTGGCGGC CCCGCAGTGA CAGCCTCATC CTGGACCATC AGTGGGAGCT GGAGAAATTG 
AGCCTCCTGC AGGAGGTGGA GAAAACCAGA CACTACCTCC TCCTTAGGGA GAAGCTGGAG 
TCCACCCTGC TGCTGGGCCA GGAGTCGCTG CTGTCCTGCG GGAGTGAGGA TCTAACCGAG 
TCCTCTTCCC CCTCCACTCA CCTCGGCCTG GGCCACAACC CCGGCCCCGG CCCCGACACA 
CCCAATGAGA GGCAGAGGGA GCTGGCTGCT AAGTGTCTGC GTTTGCTTAA CCACACATTT 
AACAGGGACT ACAGCCATGT GTGTGTTAGC GCCAGTGAGA GCAAGTTGAG TGAGATGTCA 
GTGACTCTCC TGAAAGACTC TGCTTCAGTG TGTGCCGAAA GCACCCTCAC ACCCTCCTCC 
ACATGTCCTT CACTTGTGGA GAGTCGATAC ATTCACAACA CTGATCTCAG ACCCCCAACA 
CCCCGATCTC GTGCTGTGAG CCCTAATCCT GACTCTGAAA CAGATGGTAA GAAGATTCAA 
GAGCCCAGAT CAAGAACCCG CACTTTTGTG CCAGACATTC AGGAAATCCG TGTCAGCCCA 
ATTGTGTCAA AGAAAGGCTA TTTGCACTTT CTGGAGCCCC ACAGTAATGG CTGGGTTAAG 
AGATATGTGG TGGTTCGTCG GCCTTATGTC TACATCTACA ACTCAGACAG AGACACAGTG 
GAAAGGGCCA TTCTTAACCT GTCCTCAGCG CAAGTGGAGT ATAGCGAAGA CCAGCAGGCC 
ATGCTAAAGA CCCCCTACAC ATTTGCGGTA TGCACAGAGC ATCGTGGGAT ACTCCTTCAA 
GCCAGTAATG ACAAGGAGAT GCATGACTGG CTGTATGCCT TTAACCCTCT GCTTGCTGGA 
TCTATCAGGT CTAAACTGTC CAGAAGGAGA GCTGGGCAGA TGAGGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165949.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166006.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.

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CloneID	ODa11876
Clone ID Related Accession (Same CDS sequence)	XM_005165949.3
Accession Version	XM_005165949.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGGA CATTCTACCG GTTTGAGGCT 
GCTTGGGATT CATCCATGCA CAACTCTTTG CTGCTAAACC GTGTGACTCC TTACGGAGAG 
AAAATCTACA TGACTCTGTC TGCTTACCTG GAGATGGAGA ACTGCACACA ACCCACAGTG 
ATTACCAAAG ACTTATGCAT GGTGTTCCAC TCAAGAGACG CCAAACTTCC AGCATCTCGC 
TCCATCAGAA ACCTCTTCAG CAGTGGCACC TTAAGGCCGT CTGAAGGAAA CCGTGTAACC 
GGTGTGTATG AGCTCAGTTT ATGCCACCTG GCTGATGCAG GGAGCCCAGG AATGCAAAGG 
CGGCGCAGGA GGGTGCTGGA CACGTCGGTG GCTTATGTGC GAGGGGAGGA GAACCTGGCT 
GGCTGGCGGC CCCGCAGTGA CAGCCTCATC CTGGACCATC AGTGGGAGCT GGAGAAATTG 
AGCCTCCTGC AGGAGGTGGA GAAAACCAGA CACTACCTCC TCCTTAGGGA GAAGCTGGAG 
TCCACCCTGC TGCTGGGCCA GGAGTCGCTG CTGTCCTGCG GGAGTGAGGA TCTAACCGAG 
TCCTCTTCCC CCTCCACTCA CCTCGGCCTG GGCCACAACC CCGGCCCCGG CCCCGACACA 
CCCAATGAGA GGCAGAGGGA GCTGGCTGCT AAGTGTCTGC GTTTGCTTAA CCACACATTT 
AACAGGGACT ACAGCCATGT GTGTGTTAGC GCCAGTGAGA GCAAGTTGAG TGAGATGTCA 
GTGACTCTCC TGAAAGACTC TGCTTCAGTG TGTGCCGAAA GCACCCTCAC ACCCTCCTCC 
ACATGTCCTT CACTTGTGGA GAGTCGATAC ATTCACAACA CTGATCTCAG ACCCCCAACA 
CCCCGATCTC GTGCTGTGAG CCCTAATCCT GACTCTGAAA CAGATGGTAA GAAGATTCAA 
GAGCCCAGAT CAAGAACCCG CACTTTTGTG CCAGACATTC AGGAAATCCG TGTCAGCCCA 
ATTGTGTCAA AGAAAGGCTA TTTGCACTTT CTGGAGCCCC ACAGTAATGG CTGGGTTAAG 
AGATATGTGG TGGTTCGTCG GCCTTATGTC TACATCTACA ACTCAGACAG AGACACAGTG 
GAAAGGGCCA TTCTTAACCT GTCCTCAGCG CAAGTGGAGT ATAGCGAAGA CCAGCAGGCC 
ATGCTAAAGA CCCCCTACAC ATTTGCGGTA TGCACAGAGC ATCGTGGGAT ACTCCTTCAA 
GCCAGTAATG ACAAGGAGAT GCATGACTGG CTGTATGCCT TTAACCCTCT GCTTGCTGGA 
TCTATCAGGT CTAAACTGTC CAGAAGGAGA GCTGGGCAGA TGAGGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166006.1
CDS	350..5800
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165949.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165949.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGG TGCTCTGGTT TTTCCTCCAG CCGATGAAGA GGCACCAGAC 
TATGGCTCTG GTGTCAGACA GTCAGGCACG GCCAAGATTT CTTTTGAGGA CCAGCAGTTT 
GAGAAGTTCC AGTCAGAGTC ATGTCATTCT GGTCTTTCGC GTGCTGCCGT TTCTCAGGAA 
GAGTTGAGGA TTGTGGAGGG TGAAGGACAA AGTGCAGAGA TGGGACTGTC AGCAGATGAG 
GTCAACAATA ATACATGTGC AGCTAACCCA GAGGAGCTGG ACAGCCCTGT CAAAACAGGT 
CTTGATGGGA TGCTGGACGG CGCTCTGGAT CACTTGAGGA TTGGTGAAGT CTTCACATTC 
AGAGTTACTG TTCTACAGGC GTCAAGCATT TCCGCAGAGT ACGCAGACAT ATTCTGCCAG 
TTCAATTTTA TTCACAGGCA CGATGAAGCA TTTTCCACCG AGCCGTTGAA GAACACTGGA 
CGTGGTCCAC CACTTGGCTT CTATCATGTG CAGAATATTG CAGTGGAAGT GACTAAGTCC 
TTTATTGAGT ACATAAAAAC TCAGCCAATC GTGTTTGAAG TCTTTGGACA CTACCAGAAG 
CAGCCTTTCC CTGCGCTCTG CAAGGATCTC ATTAGCCCAT TGCGTCCATG CAGACGACAG 
TTCCCAAGGG TCATGCCTCT TTCCAAACCA GTCCCTGCTA CCAAACTGAC AGCCCTCACT 
CGACCCACAG CAGGACCATG CCACTGTAAA TATGACCTGA TGGTCTTCTT TGAGATCTGC 
GAGCTGGAGG CTAATGGAGA CTATATCCCA GCCGTAGTGG ACCACCGAGT AGGCATGCCT 
TGCCATGGCA CATTCATGTT ACATCAGGGC ATCCAAAGGA GAGTTACAGT CACCATGGTA 
CATGAGACAG GAAGAGACAT TGACTGGAAG GAAGTGCGAG AGCTGGTTGT AGGACGTATC 
CGGAACACGC CTGAAGCAGA TGAGGCAATC ATCGACCCCA ATATTCTCTC TCTGAACATA 
CTGTCTGCAG GATACATTCG ACCCGCGCAT GATGACAGGA CATTCTACCG GTTTGAGGCT 
GCTTGGGATT CATCCATGCA CAACTCTTTG CTGCTAAACC GTGTGACTCC TTACGGAGAG 
AAAATCTACA TGACTCTGTC TGCTTACCTG GAGATGGAGA ACTGCACACA ACCCACAGTG 
ATTACCAAAG ACTTATGCAT GGTGTTCCAC TCAAGAGACG CCAAACTTCC AGCATCTCGC 
TCCATCAGAA ACCTCTTCAG CAGTGGCACC TTAAGGCCGT CTGAAGGAAA CCGTGTAACC 
GGTGTGTATG AGCTCAGTTT ATGCCACCTG GCTGATGCAG GGAGCCCAGG AATGCAAAGG 
CGGCGCAGGA GGGTGCTGGA CACGTCGGTG GCTTATGTGC GAGGGGAGGA GAACCTGGCT 
GGCTGGCGGC CCCGCAGTGA CAGCCTCATC CTGGACCATC AGTGGGAGCT GGAGAAATTG 
AGCCTCCTGC AGGAGGTGGA GAAAACCAGA CACTACCTCC TCCTTAGGGA GAAGCTGGAG 
TCCACCCTGC TGCTGGGCCA GGAGTCGCTG CTGTCCTGCG GGAGTGAGGA TCTAACCGAG 
TCCTCTTCCC CCTCCACTCA CCTCGGCCTG GGCCACAACC CCGGCCCCGG CCCCGACACA 
CCCAATGAGA GGCAGAGGGA GCTGGCTGCT AAGTGTCTGC GTTTGCTTAA CCACACATTT 
AACAGGGACT ACAGCCATGT GTGTGTTAGC GCCAGTGAGA GCAAGTTGAG TGAGATGTCA 
GTGACTCTCC TGAAAGACTC TGCTTCAGTG TGTGCCGAAA GCACCCTCAC ACCCTCCTCC 
ACATGTCCTT CACTTGTGGA GAGTCGATAC ATTCACAACA CTGATCTCAG ACCCCCAACA 
CCCCGATCTC GTGCTGTGAG CCCTAATCCT GACTCTGAAA CAGATGGTAA GAAGATTCAA 
GAGCCCAGAT CAAGAACCCG CACTTTTGTG CCAGACATTC AGGAAATCCG TGTCAGCCCA 
ATTGTGTCAA AGAAAGGCTA TTTGCACTTT CTGGAGCCCC ACAGTAATGG CTGGGTTAAG 
AGATATGTGG TGGTTCGTCG GCCTTATGTC TACATCTACA ACTCAGACAG AGACACAGTG 
GAAAGGGCCA TTCTTAACCT GTCCTCAGCG CAAGTGGAGT ATAGCGAAGA CCAGCAGGCC 
ATGCTAAAGA CCCCCTACAC ATTTGCGGTA TGCACAGAGC ATCGTGGGAT ACTCCTTCAA 
GCCAGTAATG ACAAGGAGAT GCATGACTGG CTGTATGCCT TTAACCCTCT GCTTGCTGGA 
TCTATCAGGT CTAAACTGTC CAGAAGGAGA GCTGGGCAGA TGAGGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165950.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166007.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X11, mRNA.

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CloneID	ODa11877
Clone ID Related Accession (Same CDS sequence)	XM_005165950.4 , XM_005165950.3
Accession Version	XM_005165950.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165950.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166007.1
CDS	352..5781
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165950.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165950.4

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165950.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166007.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.

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CloneID	ODa11877
Clone ID Related Accession (Same CDS sequence)	XM_005165950.4 , XM_005165950.3
Accession Version	XM_005165950.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166007.1
CDS	350..5779
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165950.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165950.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGAAA TCCCCTTTTC TCCACATGCC TTAGTGAGCA AGCCAGCGAC 
CCCAACTCCT CCCCCACCCC CTCTGAACCC ACCTCCACCT CTGAACTCAC CGAGCTCGTC 
CAGTCATGGC CAGACAAGAA GGTGGGGACA GAGCTGGATG ACGATGGTTT CTTATCAGAC 
GACCCGTGCT CGGATTTAGA GGAGGAGGGG GAGGATGATG ATGATGATGA GGTTGATGAT 
GACGAAGAGC TCTGCAGAAG CTCCGGCAAA GCTGAGGACC CGTTTTATGA TCGTTCACCA 
TTGTTCAGTG TAGTAGGAAG GGCTTTTGTC TATTTGAGTA ATCTGCTGTA TCCTGTGCCT 
CTGGTCCATC GGGTTGCCAT CGTCAATGAG AAGGGAGAAG TCAAAGGTTT CCTCAGAGTA 
GCTGTACAGG CAATATCAGC CGATGAAGAG GCACCAGACT ATGGCTCTGG TGTCAGACAG 
TCAGGCACGG CCAAGATTTC TTTTGAGGAC CAGCAGTTTG AGAAGTTCCA GTCAGAGTCA 
TGTCATTCTG GTCTTTCGCG TGCTGCCGTT TCTCAGGAAG AGTTGAGGAT TGTGGAGGGT 
GAAGGACAAA GTGCAGAGAT GGGACTGTCA GCAGATGAGG TCAACAATAA TACATGTGCA 
GCTAACCCAG AGGAGCTGGA CAGCCCTGTC AAAACAGGTC TTGATGGGAT GCTGGACGGC 
GCTCTGGATC ACTTGAGGAT TGGTGAAGTC TTCACATTCA GAGTTACTGT TCTACAGGCG 
TCAAGCATTT CCGCAGAGTA CGCAGACATA TTCTGCCAGT TCAATTTTAT TCACAGGCAC 
GATGAAGCAT TTTCCACCGA GCCGTTGAAG AACACTGGAC GTGGTCCACC ACTTGGCTTC 
TATCATGTGC AGAATATTGC AGTGGAAGTG ACTAAGTCCT TTATTGAGTA CATAAAAACT 
CAGCCAATCG TGTTTGAAGT CTTTGGACAC TACCAGAAGC AGCCTTTCCC TGCGCTCTGC 
AAGGATCTCA TTAGCCCATT GCGTCCATGC AGACGACAGT TCCCAAGGGT CATGCCTCTT 
TCCAAACCAG TCCCTGCTAC CAAACTGACA GCCCTCACTC GACCCACAGC AGGACCATGC 
CACTGTAAAT ATGACCTGAT GGTCTTCTTT GAGATCTGCG AGCTGGAGGC TAATGGAGAC 
TATATCCCAG CCGTAGTGGA CCACCGAGTA GGCATGCCTT GCCATGGCAC ATTCATGTTA 
CATCAGGGCA TCCAAAGGAG AGTTACAGTC ACCATGGTAC ATGAGACAGG AAGAGACATT 
GACTGGAAGG AAGTGCGAGA GCTGGTTGTA GGACGTATCC GGAACACGCC TGAAGCAGAT 
GAGGCAATCA TCGACCCCAA TATTCTCTCT CTGAACATAC TGTCTGCAGG ATACATTCGA 
CCCGCGCATG ATGACAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165951.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166008.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.

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CloneID	ODa11878
Clone ID Related Accession (Same CDS sequence)	XM_005165951.3
Accession Version	XM_005165951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATT GACCAATGCT ATGACTGGGA TGAGCCCCTC TCCATCACTC 
TCTGCCCTGT CCAGCCGGGC CGGTTCCATT AGCAGCCTGC ACGATCGCAT CATGTTCAGC 
CCCGGGAGCG AAGAAGCCAT TGAGAGACTC AAGGAAACTG AGAAAATCAT TGCAGAGCTC 
AATGAAACAT GGGAGGAGAA GCTTCGCCGT ACAGAGGCCA TCAGGATGGA CAGAGAAGCA 
CTTCTGGCGG AGATGGGTGT TGCCATGCGT GAGGATGGAG GAACTCTTGG AGTCTTTTCC 
CCAAAAAAGA CTCCCCACCT AGTGAATCTC AATGAAGATC CCTTGATGTC TGAATGTCTT 
CTATACTACA TCAAAGATGG GATTACCAGG GTGGGCAGAG AAGATGCCAG TAGTCGGCAG 
GACATTGTTC TGAGCGGACA CTTCATTAAG GAGGAACATT GTACTTTCAC CAGCACCACT 
GGACCAATGG GAGAAGCTGT CATTCTTGAG CCATGTGAGG GAGCAGAAAC ATATGTAAAT 
GGCAAGAGAG TCACAGAACC CACTGTTCTC AAATCAGGCA ACCGAATCAT TTTGGGCAAG 
AGCCACGTGT TCCGCTTCAA CCATCCAGAA CAGGCCCGTC AGGAACGTGA CCGAACCCCC 
TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT 
AAACAGGGCA TTGACATGAA GCAGGAGATG GATCAGAGAC TGCAGGAACT TGAAGATCAG 
TATCGCAAGG AGAGAGAAGA GGCCAACAAT CTACTGGAGC AACAGAGACT AGATTATGAG 
AGCAGGTTAG AGGCTCTGCA GAAACAGGTC GATCGATGTT ATCCTGACGC AGCAGAGGAA 
GATGAAGAGC CTGAGGAGGA AGTGCAATGG ACGGAGAGGG AGACGGAACT GGCAATGTGG 
GCTTTCCGCA AATGGAGATG TTATCAGTTT ACATCCCTTC GAGATCTTCT TTGGGGCAAT 
GCCATCTTCC TTAAAGAGGC CAATGCCATT AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC 
CAGTTCGTGT TATTGACGGA CACACTTTAC TCTCCTCTTC CGCCTGACCT GTTACCGCCT 
GAAGCAGCTA AAGACAGAGA GAAAAGCCCA TTTCCACGCA CCATTGTAGC TGTTGAAGTT 
CAAGATCAGA AAAACGGAGC CACGCATTAC TGGACCCTGG AAAAGCTCAG GCAGAGACTG 
GATCTGATGA GAGAGATGTA TGATCGAGCT GCTGAGGTGC CCAGCAATGC TGTTGAAGAC 
TGTGACAGTG TGCTGACCGG AGGAGATCCT TTCTACGACC GCTTTCCCTG GTTTCGTCTG 
GTTGGAAGAA ATCCCCTTTT CTCCACATGC CTTAGTGAGC AAGCCAGCGA CCCCAACTCC 
TCCCCCACCC CCTCTGAACC CACCTCCACC TCTGAACTCA CCGAGCTCGT CCAGTCATGG 
CCAGACAAGA AGGTGGGGAC AGAGCTGGAT GACGATGGTT TCTTATCAGA CGACCCGTGC 
TCGGATTTAG AGGAGGAGGG GGAGGATGAT GATGATGATG AGGTTGATGA TGACGAAGAG 
CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC CCGTTTTATG ATCGTTCACC ATTGTTCAGT 
GTAGTAGGAA GGGCTTTTGT CTATTTGAGT AATCTGCTGT ATCCTGTGCC TCTGGTCCAT 
CGGGTTGCCA TCGTCAATGA GAAGGGAGAA GTCAAAGGTT TCCTCAGAGT AGCTGTACAG 
GCAATATCAG GTGCTCTGGT TTTTCCTCCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166008.1
CDS	350..5758
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKLTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165951.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165951.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATT GACCAATGCT ATGACTGGGA TGAGCCCCTC TCCATCACTC 
TCTGCCCTGT CCAGCCGGGC CGGTTCCATT AGCAGCCTGC ACGATCGCAT CATGTTCAGC 
CCCGGGAGCG AAGAAGCCAT TGAGAGACTC AAGGAAACTG AGAAAATCAT TGCAGAGCTC 
AATGAAACAT GGGAGGAGAA GCTTCGCCGT ACAGAGGCCA TCAGGATGGA CAGAGAAGCA 
CTTCTGGCGG AGATGGGTGT TGCCATGCGT GAGGATGGAG GAACTCTTGG AGTCTTTTCC 
CCAAAAAAGA CTCCCCACCT AGTGAATCTC AATGAAGATC CCTTGATGTC TGAATGTCTT 
CTATACTACA TCAAAGATGG GATTACCAGG GTGGGCAGAG AAGATGCCAG TAGTCGGCAG 
GACATTGTTC TGAGCGGACA CTTCATTAAG GAGGAACATT GTACTTTCAC CAGCACCACT 
GGACCAATGG GAGAAGCTGT CATTCTTGAG CCATGTGAGG GAGCAGAAAC ATATGTAAAT 
GGCAAGAGAG TCACAGAACC CACTGTTCTC AAATCAGGCA ACCGAATCAT TTTGGGCAAG 
AGCCACGTGT TCCGCTTCAA CCATCCAGAA CAGGCCCGTC AGGAACGTGA CCGAACCCCC 
TGCGCTGAGA CCCCAGTGGA GCCGGTAGAC TGGGCCTTTG CTCAGAGAGA ACTGCTGGAT 
AAACAGGGCA TTGACATGAA GCAGGAGATG GATCAGAGAC TGCAGGAACT TGAAGATCAG 
TATCGCAAGG AGAGAGAAGA GGCCAACAAT CTACTGGAGC AACAGAGACT AGATTATGAG 
AGCAGGTTAG AGGCTCTGCA GAAACAGGTC GATCGATGTT ATCCTGACGC AGCAGAGGAA 
GATGAAGAGC CTGAGGAGGA AGTGCAATGG ACGGAGAGGG AGACGGAACT GGCAATGTGG 
GCTTTCCGCA AATGGAGATG TTATCAGTTT ACATCCCTTC GAGATCTTCT TTGGGGCAAT 
GCCATCTTCC TTAAAGAGGC CAATGCCATT AGTGTGGAGC TAAAGAAGAA GGTTCAGTTC 
CAGTTCGTGT TATTGACGGA CACACTTTAC TCTCCTCTTC CGCCTGACCT GTTACCGCCT 
GAAGCAGCTA AAGACAGAGA GAAAAGCCCA TTTCCACGCA CCATTGTAGC TGTTGAAGTT 
CAAGATCAGA AAAACGGAGC CACGCATTAC TGGACCCTGG AAAAGCTCAG GCAGAGACTG 
GATCTGATGA GAGAGATGTA TGATCGAGCT GCTGAGGTGC CCAGCAATGC TGTTGAAGAC 
TGTGACAGTG TGCTGACCGG AGGAGATCCT TTCTACGACC GCTTTCCCTG GTTTCGTCTG 
GTTGGAAGAA ATCCCCTTTT CTCCACATGC CTTAGTGAGC AAGCCAGCGA CCCCAACTCC 
TCCCCCACCC CCTCTGAACC CACCTCCACC TCTGAACTCA CCGAGCTCGT CCAGTCATGG 
CCAGACAAGA AGGTGGGGAC AGAGCTGGAT GACGATGGTT TCTTATCAGA CGACCCGTGC 
TCGGATTTAG AGGAGGAGGG GGAGGATGAT GATGATGATG AGGTTGATGA TGACGAAGAG 
CTCTGCAGAA GCTCCGGCAA AGCTGAGGAC CCGTTTTATG ATCGTTCACC ATTGTTCAGT 
GTAGTAGGAA GGGCTTTTGT CTATTTGAGT AATCTGCTGT ATCCTGTGCC TCTGGTCCAT 
CGGGTTGCCA TCGTCAATGA GAAGGGAGAA GTCAAAGGTT TCCTCAGAGT AGCTGTACAG 
GCAATATCAG GTGCTCTGGT TTTTCCTCCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165952.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166009.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.

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CloneID	ODa11879
Clone ID Related Accession (Same CDS sequence)	XM_005165952.3
Accession Version	XM_005165952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GGTGCTCTGG TTTTTCCTCC AGCCGATGAA 
GAGGCACCAG ACTATGGCTC TGGTGTCAGA CAGTCAGGCA CGGCCAAGAT TTCTTTTGAG 
GACCAGCAGT TTGAGAAGTT CCAGTCAGAG TCATGTCATT CTGGTCTTTC GCGTGCTGCC 
GTTTCTCAGG AAGAGTTGAG GATTGTGGAG GGTGAAGGAC AAAGTGCAGA GATGGGACTG 
TCAGCAGATG AGGTCAACAA TAATACATGT GCAGCTAACC CAGAGGAGCT GGACAGCCCT 
GTCAAAACAG GTCTTGATGG GATGCTGGAC GGCGCTCTGG ATCACTTGAG GATTGGTGAA 
GTCTTCACAT TCAGAGTTAC TGTTCTACAG GCGTCAAGCA TTTCCGCAGA GTACGCAGAC 
ATATTCTGCC AGTTCAATTT TATTCACAGG CACGATGAAG CATTTTCCAC CGAGCCGTTG 
AAGAACACTG GACGTGGTCC ACCACTTGGC TTCTATCATG TGCAGAATAT TGCAGTGGAA 
GTGACTAAGT CCTTTATTGA GTACATAAAA ACTCAGCCAA TCGTGTTTGA AGTCTTTGGA 
CACTACCAGA AGCAGCCTTT CCCTGCGCTC TGCAAGGATC TCATTAGCCC ATTGCGTCCA 
TGCAGACGAC AGTTCCCAAG GGTCATGCCT CTTTCCAAAC CAGTCCCTGC TACCAAACTG 
ACAGCCCTCA CTCGACCCAC AGCAGGACCA TGCCACTGTA AATATGACCT GATGGTCTTC 
TTTGAGATCT GCGAGCTGGA GGCTAATGGA GACTATATCC CAGCCGTAGT GGACCACCGA 
GTAGGCATGC CTTGCCATGG CACATTCATG TTACATCAGG GCATCCAAAG GAGAGTTACA 
GTCACCATGG TACATGAGAC AGGAAGAGAC ATTGACTGGA AGGAAGTGCG AGAGCTGGTT 
GTAGGACGTA TCCGGAACAC GCCTGAAGCA GATGAGGCAA TCATCGACCC CAATATTCTC 
TCTCTGAACA TACTGTCTGC AGGATACATT CGACCCGCGC ATGATGACAG ACAGTTTCTT 
GATTCGGACA TGCCTAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166009.1
CDS	350..5719
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165952.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165952.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GGTGCTCTGG TTTTTCCTCC AGCCGATGAA 
GAGGCACCAG ACTATGGCTC TGGTGTCAGA CAGTCAGGCA CGGCCAAGAT TTCTTTTGAG 
GACCAGCAGT TTGAGAAGTT CCAGTCAGAG TCATGTCATT CTGGTCTTTC GCGTGCTGCC 
GTTTCTCAGG AAGAGTTGAG GATTGTGGAG GGTGAAGGAC AAAGTGCAGA GATGGGACTG 
TCAGCAGATG AGGTCAACAA TAATACATGT GCAGCTAACC CAGAGGAGCT GGACAGCCCT 
GTCAAAACAG GTCTTGATGG GATGCTGGAC GGCGCTCTGG ATCACTTGAG GATTGGTGAA 
GTCTTCACAT TCAGAGTTAC TGTTCTACAG GCGTCAAGCA TTTCCGCAGA GTACGCAGAC 
ATATTCTGCC AGTTCAATTT TATTCACAGG CACGATGAAG CATTTTCCAC CGAGCCGTTG 
AAGAACACTG GACGTGGTCC ACCACTTGGC TTCTATCATG TGCAGAATAT TGCAGTGGAA 
GTGACTAAGT CCTTTATTGA GTACATAAAA ACTCAGCCAA TCGTGTTTGA AGTCTTTGGA 
CACTACCAGA AGCAGCCTTT CCCTGCGCTC TGCAAGGATC TCATTAGCCC ATTGCGTCCA 
TGCAGACGAC AGTTCCCAAG GGTCATGCCT CTTTCCAAAC CAGTCCCTGC TACCAAACTG 
ACAGCCCTCA CTCGACCCAC AGCAGGACCA TGCCACTGTA AATATGACCT GATGGTCTTC 
TTTGAGATCT GCGAGCTGGA GGCTAATGGA GACTATATCC CAGCCGTAGT GGACCACCGA 
GTAGGCATGC CTTGCCATGG CACATTCATG TTACATCAGG GCATCCAAAG GAGAGTTACA 
GTCACCATGG TACATGAGAC AGGAAGAGAC ATTGACTGGA AGGAAGTGCG AGAGCTGGTT 
GTAGGACGTA TCCGGAACAC GCCTGAAGCA GATGAGGCAA TCATCGACCC CAATATTCTC 
TCTCTGAACA TACTGTCTGC AGGATACATT CGACCCGCGC ATGATGACAG ACAGTTTCTT 
GATTCGGACA TGCCTAGGAC ATTCTACCGG TTTGAGGCTG CTTGGGATTC ATCCATGCAC 
AACTCTTTGC TGCTAAACCG TGTGACTCCT TACGGAGAGA AAATCTACAT GACTCTGTCT 
GCTTACCTGG AGATGGAGAA CTGCACACAA CCCACAGTGA TTACCAAAGA CTTATGCATG 
GTGTTCCACT CAAGAGACGC CAAACTTCCA GCATCTCGCT CCATCAGAAA CCTCTTCAGC 
AGTGGCACCT TAAGGCCGTC TGAAGGAAAC CGTGTAACCG GTGTGTATGA GCTCAGTTTA 
TGCCACCTGG CTGATGCAGG GAGCCCAGGA ATGCAAAGGC GGCGCAGGAG GGTGCTGGAC 
ACGTCGGTGG CTTATGTGCG AGGGGAGGAG AACCTGGCTG GCTGGCGGCC CCGCAGTGAC 
AGCCTCATCC TGGACCATCA GTGGGAGCTG GAGAAATTGA GCCTCCTGCA GGAGGTGGAG 
AAAACCAGAC ACTACCTCCT CCTTAGGGAG AAGCTGGAGT CCACCCTGCT GCTGGGCCAG 
GAGTCGCTGC TGTCCTGCGG GAGTGAGGAT CTAACCGAGT CCTCTTCCCC CTCCACTCAC 
CTCGGCCTGG GCCACAACCC CGGCCCCGGC CCCGACACAC CCAATGAGAG GCAGAGGGAG 
CTGGCTGCTA AGTGTCTGCG TTTGCTTAAC CACACATTTA ACAGGGACTA CAGCCATGTG 
TGTGTTAGCG CCAGTGAGAG CAAGTTGAGT GAGATGTCAG TGACTCTCCT GAAAGACTCT 
GCTTCAGTGT GTGCCGAAAG CACCCTCACA CCCTCCTCCA CATGTCCTTC ACTTGTGGAG 
AGTCGATACA TTCACAACAC TGATCTCAGA CCCCCAACAC CCCGATCTCG TGCTGTGAGC 
CCTAATCCTG ACTCTGAAAC AGATGGTAAG AAGATTCAAG AGCCCAGATC AAGAACCCGC 
ACTTTTGTGC CAGACATTCA GGAAATCCGT GTCAGCCCAA TTGTGTCAAA GAAAGGCTAT 
TTGCACTTTC TGGAGCCCCA CAGTAATGGC TGGGTTAAGA GATATGTGGT GGTTCGTCGG 
CCTTATGTCT ACATCTACAA CTCAGACAGA GACACAGTGG AAAGGGCCAT TCTTAACCTG 
TCCTCAGCGC AAGTGGAGTA TAGCGAAGAC CAGCAGGCCA TGCTAAAGAC CCCCTACACA 
TTTGCGGTAT GCACAGAGCA TCGTGGGATA CTCCTTCAAG CCAGTAATGA CAAGGAGATG 
CATGACTGGC TGTATGCCTT TAACCCTCTG CTTGCTGGAT CTATCAGGTC TAAACTGTCC 
AGAAGGAGAG CTGGGCAGAT GAGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165953.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166010.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.

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CloneID	ODa11880
Clone ID Related Accession (Same CDS sequence)	XM_005165953.3
Accession Version	XM_005165953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGGTGC TCTGGTTTTT CCTCCAGCCG ATGAAGAGGC ACCAGACTAT 
GGCTCTGGTG TCAGACAGTC AGGCACGGCC AAGATTTCTT TTGAGGACCA GCAGTTTGAG 
AAGTTCCAGT CAGAGTCATG TCATTCTGGT CTTTCGCGTG CTGCCGTTTC TCAGGAAGAG 
TTGAGGATTG TGGAGGGTGA AGGACAAAGT GCAGAGATGG GACTGTCAGC AGATGAGGTC 
AACAATAATA CATGTGCAGC TAACCCAGAG GAGCTGGACA GCCCTGTCAA AACAGGTCTT 
GATGGGATGC TGGACGGCGC TCTGGATCAC TTGAGGATTG GTGAAGTCTT CACATTCAGA 
GTTACTGTTC TACAGGCGTC AAGCATTTCC GCAGAGTACG CAGACATATT CTGCCAGTTC 
AATTTTATTC ACAGGCACGA TGAAGCATTT TCCACCGAGC CGTTGAAGAA CACTGGACGT 
GGTCCACCAC TTGGCTTCTA TCATGTGCAG AATATTGCAG TGGAAGTGAC TAAGTCCTTT 
ATTGAGTACA TAAAAACTCA GCCAATCGTG TTTGAAGTCT TTGGACACTA CCAGAAGCAG 
CCTTTCCCTG CGCTCTGCAA GGATCTCATT AGCCCATTGC GTCCATGCAG ACGACAGTTC 
CCAAGGGTCA TGCCTCTTTC CAAACCAGTC CCTGCTACCA AACTGACAGC CCTCACTCGA 
CCCACAGCAG GACCATGCCA CTGTAAATAT GACCTGATGG TCTTCTTTGA GATCTGCGAG 
CTGGAGGCTA ATGGAGACTA TATCCCAGCC GTAGTGGACC ACCGAGTAGG CATGCCTTGC 
CATGGCACAT TCATGTTACA TCAGGGCATC CAAAGGAGAG TTACAGTCAC CATGGTACAT 
GAGACAGGAA GAGACATTGA CTGGAAGGAA GTGCGAGAGC TGGTTGTAGG ACGTATCCGG 
AACACGCCTG AAGCAGATGA GGCAATCATC GACCCCAATA TTCTCTCTCT GAACATACTG 
TCTGCAGGAT ACATTCGACC CGCGCATGAT GACAGACAGT TTCTTGATTC GGACATGCCT 
AGGACATTCT ACCGGTTTGA GGCTGCTTGG GATTCATCCA TGCACAACTC TTTGCTGCTA 
AACCGTGTGA CTCCTTACGG AGAGAAAATC TACATGACTC TGTCTGCTTA CCTGGAGATG 
GAGAACTGCA CACAACCCAC AGTGATTACC AAAGACTTAT GCATGGTGTT CCACTCAAGA 
GACGCCAAAC TTCCAGCATC TCGCTCCATC AGAAACCTCT TCAGCAGTGG CACCTTAAGG 
CCGTCTGAAG GAAACCGTGT AACCGGTGTG TATGAGCTCA GTTTATGCCA CCTGGCTGAT 
GCAGGGAGCC CAGGAATGCA AAGGCGGCGC AGGAGGGTGC TGGACACGTC GGTGGCTTAT 
GTGCGAGGGG AGGAGAACCT GGCTGGCTGG CGGCCCCGCA GTGACAGCCT CATCCTGGAC 
CATCAGTGGG AGCTGGAGAA ATTGAGCCTC CTGCAGGAGG TGGAGAAAAC CAGACACTAC 
CTCCTCCTTA GGGAGAAGCT GGAGTCCACC CTGCTGCTGG GCCAGGAGTC GCTGCTGTCC 
TGCGGGAGTG AGGATCTAAC CGAGTCCTCT TCCCCCTCCA CTCACCTCGG CCTGGGCCAC 
AACCCCGGCC CCGGCCCCGA CACACCCAAT GAGAGGCAGA GGGAGCTGGC TGCTAAGTGT 
CTGCGTTTGC TTAACCACAC ATTTAACAGG GACTACAGCC ATGTGTGTGT TAGCGCCAGT 
GAGAGCAAGT TGAGTGAGAT GTCAGTGACT CTCCTGAAAG ACTCTGCTTC AGTGTGTGCC 
GAAAGCACCC TCACACCCTC CTCCACATGT CCTTCACTTG TGGAGAGTCG ATACATTCAC 
AACACTGATC TCAGACCCCC AACACCCCGA TCTCGTGCTG TGAGCCCTAA TCCTGACTCT 
GAAACAGATG GTAAGAAGAT TCAAGAGCCC AGATCAAGAA CCCGCACTTT TGTGCCAGAC 
ATTCAGGAAA TCCGTGTCAG CCCAATTGTG TCAAAGAAAG GCTATTTGCA CTTTCTGGAG 
CCCCACAGTA ATGGCTGGGT TAAGAGATAT GTGGTGGTTC GTCGGCCTTA TGTCTACATC 
TACAACTCAG ACAGAGACAC AGTGGAAAGG GCCATTCTTA ACCTGTCCTC AGCGCAAGTG 
GAGTATAGCG AAGACCAGCA GGCCATGCTA AAGACCCCCT ACACATTTGC GGTATGCACA 
GAGCATCGTG GGATACTCCT TCAAGCCAGT AATGACAAGG AGATGCATGA CTGGCTGTAT 
GCCTTTAACC CTCTGCTTGC TGGATCTATC AGGTCTAAAC TGTCCAGAAG GAGAGCTGGG 
CAGATGAGGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166010.1
CDS	350..5524
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISGALVFPPADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165953.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165953.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGGTGC TCTGGTTTTT CCTCCAGCCG ATGAAGAGGC ACCAGACTAT 
GGCTCTGGTG TCAGACAGTC AGGCACGGCC AAGATTTCTT TTGAGGACCA GCAGTTTGAG 
AAGTTCCAGT CAGAGTCATG TCATTCTGGT CTTTCGCGTG CTGCCGTTTC TCAGGAAGAG 
TTGAGGATTG TGGAGGGTGA AGGACAAAGT GCAGAGATGG GACTGTCAGC AGATGAGGTC 
AACAATAATA CATGTGCAGC TAACCCAGAG GAGCTGGACA GCCCTGTCAA AACAGGTCTT 
GATGGGATGC TGGACGGCGC TCTGGATCAC TTGAGGATTG GTGAAGTCTT CACATTCAGA 
GTTACTGTTC TACAGGCGTC AAGCATTTCC GCAGAGTACG CAGACATATT CTGCCAGTTC 
AATTTTATTC ACAGGCACGA TGAAGCATTT TCCACCGAGC CGTTGAAGAA CACTGGACGT 
GGTCCACCAC TTGGCTTCTA TCATGTGCAG AATATTGCAG TGGAAGTGAC TAAGTCCTTT 
ATTGAGTACA TAAAAACTCA GCCAATCGTG TTTGAAGTCT TTGGACACTA CCAGAAGCAG 
CCTTTCCCTG CGCTCTGCAA GGATCTCATT AGCCCATTGC GTCCATGCAG ACGACAGTTC 
CCAAGGGTCA TGCCTCTTTC CAAACCAGTC CCTGCTACCA AACTGACAGC CCTCACTCGA 
CCCACAGCAG GACCATGCCA CTGTAAATAT GACCTGATGG TCTTCTTTGA GATCTGCGAG 
CTGGAGGCTA ATGGAGACTA TATCCCAGCC GTAGTGGACC ACCGAGTAGG CATGCCTTGC 
CATGGCACAT TCATGTTACA TCAGGGCATC CAAAGGAGAG TTACAGTCAC CATGGTACAT 
GAGACAGGAA GAGACATTGA CTGGAAGGAA GTGCGAGAGC TGGTTGTAGG ACGTATCCGG 
AACACGCCTG AAGCAGATGA GGCAATCATC GACCCCAATA TTCTCTCTCT GAACATACTG 
TCTGCAGGAT ACATTCGACC CGCGCATGAT GACAGACAGT TTCTTGATTC GGACATGCCT 
AGGACATTCT ACCGGTTTGA GGCTGCTTGG GATTCATCCA TGCACAACTC TTTGCTGCTA 
AACCGTGTGA CTCCTTACGG AGAGAAAATC TACATGACTC TGTCTGCTTA CCTGGAGATG 
GAGAACTGCA CACAACCCAC AGTGATTACC AAAGACTTAT GCATGGTGTT CCACTCAAGA 
GACGCCAAAC TTCCAGCATC TCGCTCCATC AGAAACCTCT TCAGCAGTGG CACCTTAAGG 
CCGTCTGAAG GAAACCGTGT AACCGGTGTG TATGAGCTCA GTTTATGCCA CCTGGCTGAT 
GCAGGGAGCC CAGGAATGCA AAGGCGGCGC AGGAGGGTGC TGGACACGTC GGTGGCTTAT 
GTGCGAGGGG AGGAGAACCT GGCTGGCTGG CGGCCCCGCA GTGACAGCCT CATCCTGGAC 
CATCAGTGGG AGCTGGAGAA ATTGAGCCTC CTGCAGGAGG TGGAGAAAAC CAGACACTAC 
CTCCTCCTTA GGGAGAAGCT GGAGTCCACC CTGCTGCTGG GCCAGGAGTC GCTGCTGTCC 
TGCGGGAGTG AGGATCTAAC CGAGTCCTCT TCCCCCTCCA CTCACCTCGG CCTGGGCCAC 
AACCCCGGCC CCGGCCCCGA CACACCCAAT GAGAGGCAGA GGGAGCTGGC TGCTAAGTGT 
CTGCGTTTGC TTAACCACAC ATTTAACAGG GACTACAGCC ATGTGTGTGT TAGCGCCAGT 
GAGAGCAAGT TGAGTGAGAT GTCAGTGACT CTCCTGAAAG ACTCTGCTTC AGTGTGTGCC 
GAAAGCACCC TCACACCCTC CTCCACATGT CCTTCACTTG TGGAGAGTCG ATACATTCAC 
AACACTGATC TCAGACCCCC AACACCCCGA TCTCGTGCTG TGAGCCCTAA TCCTGACTCT 
GAAACAGATG GTAAGAAGAT TCAAGAGCCC AGATCAAGAA CCCGCACTTT TGTGCCAGAC 
ATTCAGGAAA TCCGTGTCAG CCCAATTGTG TCAAAGAAAG GCTATTTGCA CTTTCTGGAG 
CCCCACAGTA ATGGCTGGGT TAAGAGATAT GTGGTGGTTC GTCGGCCTTA TGTCTACATC 
TACAACTCAG ACAGAGACAC AGTGGAAAGG GCCATTCTTA ACCTGTCCTC AGCGCAAGTG 
GAGTATAGCG AAGACCAGCA GGCCATGCTA AAGACCCCCT ACACATTTGC GGTATGCACA 
GAGCATCGTG GGATACTCCT TCAAGCCAGT AATGACAAGG AGATGCATGA CTGGCTGTAT 
GCCTTTAACC CTCTGCTTGC TGGATCTATC AGGTCTAAAC TGTCCAGAAG GAGAGCTGGG 
CAGATGAGGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165954.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166011.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X15, mRNA.

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CloneID	ODa11881
Clone ID Related Accession (Same CDS sequence)	XM_005165954.4 , XM_005165954.3
Accession Version	XM_005165954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165954.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGGACATT CTACCGGTTT GAGGCTGCTT GGGATTCATC CATGCACAAC 
TCTTTGCTGC TAAACCGTGT GACTCCTTAC GGAGAGAAAA TCTACATGAC TCTGTCTGCT 
TACCTGGAGA TGGAGAACTG CACACAACCC ACAGTGATTA CCAAAGACTT ATGCATGGTG 
TTCCACTCAA GAGACGCCAA ACTTCCAGCA TCTCGCTCCA TCAGAAACCT CTTCAGCAGT 
GGCACCTTAA GGCCGTCTGA AGGAAACCGT GTAACCGGTG TGTATGAGCT CAGTTTATGC 
CACCTGGCTG ATGCAGGGAG CCCAGGAATG CAAAGGCGGC GCAGGAGGGT GCTGGACACG 
TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC CTGGCTGGCT GGCGGCCCCG CAGTGACAGC 
CTCATCCTGG ACCATCAGTG GGAGCTGGAG AAATTGAGCC TCCTGCAGGA GGTGGAGAAA 
ACCAGACACT ACCTCCTCCT TAGGGAGAAG CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG 
TCGCTGCTGT CCTGCGGGAG TGAGGATCTA ACCGAGTCCT CTTCCCCCTC CACTCACCTC 
GGCCTGGGCC ACAACCCCGG CCCCGGCCCC GACACACCCA ATGAGAGGCA GAGGGAGCTG 
GCTGCTAAGT GTCTGCGTTT GCTTAACCAC ACATTTAACA GGGACTACAG CCATGTGTGT 
GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG ATGTCAGTGA CTCTCCTGAA AGACTCTGCT 
TCAGTGTGTG CCGAAAGCAC CCTCACACCC TCCTCCACAT GTCCTTCACT TGTGGAGAGT 
CGATACATTC ACAACACTGA TCTCAGACCC CCAACACCCC GATCTCGTGC TGTGAGCCCT 
AATCCTGACT CTGAAACAGA TGGTAAGAAG ATTCAAGAGC CCAGATCAAG AACCCGCACT 
TTTGTGCCAG ACATTCAGGA AATCCGTGTC AGCCCAATTG TGTCAAAGAA AGGCTATTTG 
CACTTTCTGG AGCCCCACAG TAATGGCTGG GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT 
TATGTCTACA TCTACAACTC AGACAGAGAC ACAGTGGAAA GGGCCATTCT TAACCTGTCC 
TCAGCGCAAG TGGAGTATAG CGAAGACCAG CAGGCCATGC TAAAGACCCC CTACACATTT 
GCGGTATGCA CAGAGCATCG TGGGATACTC CTTCAAGCCA GTAATGACAA GGAGATGCAT 
GACTGGCTGT ATGCCTTTAA CCCTCTGCTT GCTGGATCTA TCAGGTCTAA ACTGTCCAGA 
AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166011.1
CDS	351..5477
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165954.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165954.4

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGGACATT CTACCGGTTT GAGGCTGCTT GGGATTCATC CATGCACAAC 
TCTTTGCTGC TAAACCGTGT GACTCCTTAC GGAGAGAAAA TCTACATGAC TCTGTCTGCT 
TACCTGGAGA TGGAGAACTG CACACAACCC ACAGTGATTA CCAAAGACTT ATGCATGGTG 
TTCCACTCAA GAGACGCCAA ACTTCCAGCA TCTCGCTCCA TCAGAAACCT CTTCAGCAGT 
GGCACCTTAA GGCCGTCTGA AGGAAACCGT GTAACCGGTG TGTATGAGCT CAGTTTATGC 
CACCTGGCTG ATGCAGGGAG CCCAGGAATG CAAAGGCGGC GCAGGAGGGT GCTGGACACG 
TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC CTGGCTGGCT GGCGGCCCCG CAGTGACAGC 
CTCATCCTGG ACCATCAGTG GGAGCTGGAG AAATTGAGCC TCCTGCAGGA GGTGGAGAAA 
ACCAGACACT ACCTCCTCCT TAGGGAGAAG CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG 
TCGCTGCTGT CCTGCGGGAG TGAGGATCTA ACCGAGTCCT CTTCCCCCTC CACTCACCTC 
GGCCTGGGCC ACAACCCCGG CCCCGGCCCC GACACACCCA ATGAGAGGCA GAGGGAGCTG 
GCTGCTAAGT GTCTGCGTTT GCTTAACCAC ACATTTAACA GGGACTACAG CCATGTGTGT 
GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG ATGTCAGTGA CTCTCCTGAA AGACTCTGCT 
TCAGTGTGTG CCGAAAGCAC CCTCACACCC TCCTCCACAT GTCCTTCACT TGTGGAGAGT 
CGATACATTC ACAACACTGA TCTCAGACCC CCAACACCCC GATCTCGTGC TGTGAGCCCT 
AATCCTGACT CTGAAACAGA TGGTAAGAAG ATTCAAGAGC CCAGATCAAG AACCCGCACT 
TTTGTGCCAG ACATTCAGGA AATCCGTGTC AGCCCAATTG TGTCAAAGAA AGGCTATTTG 
CACTTTCTGG AGCCCCACAG TAATGGCTGG GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT 
TATGTCTACA TCTACAACTC AGACAGAGAC ACAGTGGAAA GGGCCATTCT TAACCTGTCC 
TCAGCGCAAG TGGAGTATAG CGAAGACCAG CAGGCCATGC TAAAGACCCC CTACACATTT 
GCGGTATGCA CAGAGCATCG TGGGATACTC CTTCAAGCCA GTAATGACAA GGAGATGCAT 
GACTGGCTGT ATGCCTTTAA CCCTCTGCTT GCTGGATCTA TCAGGTCTAA ACTGTCCAGA 
AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165954.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166011.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.

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CloneID	ODa11881
Clone ID Related Accession (Same CDS sequence)	XM_005165954.4 , XM_005165954.3
Accession Version	XM_005165954.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGGACATT CTACCGGTTT GAGGCTGCTT GGGATTCATC CATGCACAAC 
TCTTTGCTGC TAAACCGTGT GACTCCTTAC GGAGAGAAAA TCTACATGAC TCTGTCTGCT 
TACCTGGAGA TGGAGAACTG CACACAACCC ACAGTGATTA CCAAAGACTT ATGCATGGTG 
TTCCACTCAA GAGACGCCAA ACTTCCAGCA TCTCGCTCCA TCAGAAACCT CTTCAGCAGT 
GGCACCTTAA GGCCGTCTGA AGGAAACCGT GTAACCGGTG TGTATGAGCT CAGTTTATGC 
CACCTGGCTG ATGCAGGGAG CCCAGGAATG CAAAGGCGGC GCAGGAGGGT GCTGGACACG 
TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC CTGGCTGGCT GGCGGCCCCG CAGTGACAGC 
CTCATCCTGG ACCATCAGTG GGAGCTGGAG AAATTGAGCC TCCTGCAGGA GGTGGAGAAA 
ACCAGACACT ACCTCCTCCT TAGGGAGAAG CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG 
TCGCTGCTGT CCTGCGGGAG TGAGGATCTA ACCGAGTCCT CTTCCCCCTC CACTCACCTC 
GGCCTGGGCC ACAACCCCGG CCCCGGCCCC GACACACCCA ATGAGAGGCA GAGGGAGCTG 
GCTGCTAAGT GTCTGCGTTT GCTTAACCAC ACATTTAACA GGGACTACAG CCATGTGTGT 
GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG ATGTCAGTGA CTCTCCTGAA AGACTCTGCT 
TCAGTGTGTG CCGAAAGCAC CCTCACACCC TCCTCCACAT GTCCTTCACT TGTGGAGAGT 
CGATACATTC ACAACACTGA TCTCAGACCC CCAACACCCC GATCTCGTGC TGTGAGCCCT 
AATCCTGACT CTGAAACAGA TGGTAAGAAG ATTCAAGAGC CCAGATCAAG AACCCGCACT 
TTTGTGCCAG ACATTCAGGA AATCCGTGTC AGCCCAATTG TGTCAAAGAA AGGCTATTTG 
CACTTTCTGG AGCCCCACAG TAATGGCTGG GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT 
TATGTCTACA TCTACAACTC AGACAGAGAC ACAGTGGAAA GGGCCATTCT TAACCTGTCC 
TCAGCGCAAG TGGAGTATAG CGAAGACCAG CAGGCCATGC TAAAGACCCC CTACACATTT 
GCGGTATGCA CAGAGCATCG TGGGATACTC CTTCAAGCCA GTAATGACAA GGAGATGCAT 
GACTGGCTGT ATGCCTTTAA CCCTCTGCTT GCTGGATCTA TCAGGTCTAA ACTGTCCAGA 
AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166011.1
CDS	350..5476
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIETFRGPVEDFAGLKYISDYKNHNNNGQALNQRGDFSTVTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165954.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165954.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA CGTTTCGAGG GCCAGTTGAA 
GATTTTGCTG GTTTGAAATA CATATCCGAT TACAAGAACC ATAACAATAA TGGCCAAGCT 
CTCAATCAAA GAGGTGATTT CTCCACAGTG ACCAATGCTA TGACTGGGAT GAGCCCCTCT 
CCATCACTCT CTGCCCTGTC CAGCCGGGCC GGTTCCATTA GCAGCCTGCA CGATCGCATC 
ATGTTCAGCC CCGGGAGCGA AGAAGCCATT GAGAGACTCA AGGAAACTGA GAAAATCATT 
GCAGAGCTCA ATGAAACATG GGAGGAGAAG CTTCGCCGTA CAGAGGCCAT CAGGATGGAC 
AGAGAAGCAC TTCTGGCGGA GATGGGTGTT GCCATGCGTG AGGATGGAGG AACTCTTGGA 
GTCTTTTCCC CAAAAAAGAC TCCCCACCTA GTGAATCTCA ATGAAGATCC CTTGATGTCT 
GAATGTCTTC TATACTACAT CAAAGATGGG ATTACCAGGG TGGGCAGAGA AGATGCCAGT 
AGTCGGCAGG ACATTGTTCT GAGCGGACAC TTCATTAAGG AGGAACATTG TACTTTCACC 
AGCACCACTG GACCAATGGG AGAAGCTGTC ATTCTTGAGC CATGTGAGGG AGCAGAAACA 
TATGTAAATG GCAAGAGAGT CACAGAACCC ACTGTTCTCA AATCAGGCAA CCGAATCATT 
TTGGGCAAGA GCCACGTGTT CCGCTTCAAC CATCCAGAAC AGGCCCGTCA GGAACGTGAC 
CGAACCCCCT GCGCTGAGAC CCCAGTGGAG CCGGTAGACT GGGCCTTTGC TCAGAGAGAA 
CTGCTGGATA AACAGGGCAT TGACATGAAG CAGGAGATGG ATCAGAGACT GCAGGAACTT 
GAAGATCAGT ATCGCAAGGA GAGAGAAGAG GCCAACAATC TACTGGAGCA ACAGAGACTA 
GATTATGAGA GCAGGTTAGA GGCTCTGCAG AAACAGGTCG ATCGATGTTA TCCTGACGCA 
GCAGAGGAAG ATGAAGAGCC TGAGGAGGAA GTGCAATGGA CGGAGAGGGA GACGGAACTG 
GCAATGTGGG CTTTCCGCAA ATGGAGATGT TATCAGTTTA CATCCCTTCG AGATCTTCTT 
TGGGGCAATG CCATCTTCCT TAAAGAGGCC AATGCCATTA GTGTGGAGCT AAAGAAGAAG 
GTTCAGTTCC AGTTCGTGTT ATTGACGGAC ACACTTTACT CTCCTCTTCC GCCTGACCTG 
TTACCGCCTG AAGCAGCTAA AGACAGAGAG AAAAGCCCAT TTCCACGCAC CATTGTAGCT 
GTTGAAGTTC AAGATCAGAA AAACGGAGCC ACGCATTACT GGACCCTGGA AAAGCTCAGG 
CAGAGACTGG ATCTGATGAG AGAGATGTAT GATCGAGCTG CTGAGGTGCC CAGCAATGCT 
GTTGAAGACT GTGACAGTGT GCTGACCGGA GGAGATCCTT TCTACGACCG CTTTCCCTGG 
TTTCGTCTGG TTGGAAGGGC TTTTGTCTAT TTGAGTAATC TGCTGTATCC TGTGCCTCTG 
GTCCATCGGG TTGCCATCGT CAATGAGAAG GGAGAAGTCA AAGGTTTCCT CAGAGTAGCT 
GTACAGGCAA TATCAGCCGA TGAAGAGGCA CCAGACTATG GCTCTGGTGT CAGACAGTCA 
GGCACGGCCA AGATTTCTTT TGAGGACCAG CAGTTTGAGA AGTTCCAGTC AGAGTCATGT 
CATTCTGGTC TTTCGCGTGC TGCCGTTTCT CAGGAAGAGT TGAGGATTGT GGAGGGTGAA 
GGACAAAGTG CAGAGATGGG ACTGTCAGCA GATGAGGTCA ACAATAATAC ATGTGCAGCT 
AACCCAGAGG AGCTGGACAG CCCTGTCAAA ACAGGTCTTG ATGGGATGCT GGACGGCGCT 
CTGGATCACT TGAGGATTGG TGAAGTCTTC ACATTCAGAG TTACTGTTCT ACAGGCGTCA 
AGCATTTCCG CAGAGTACGC AGACATATTC TGCCAGTTCA ATTTTATTCA CAGGCACGAT 
GAAGCATTTT CCACCGAGCC GTTGAAGAAC ACTGGACGTG GTCCACCACT TGGCTTCTAT 
CATGTGCAGA ATATTGCAGT GGAAGTGACT AAGTCCTTTA TTGAGTACAT AAAAACTCAG 
CCAATCGTGT TTGAAGTCTT TGGACACTAC CAGAAGCAGC CTTTCCCTGC GCTCTGCAAG 
GATCTCATTA GCCCATTGCG TCCATGCAGA CGACAGTTCC CAAGGGTCAT GCCTCTTTCC 
AAACCAGTCC CTGCTACCAA ACTGACAGCC CTCACTCGAC CCACAGCAGG ACCATGCCAC 
TGTAAATATG ACCTGATGGT CTTCTTTGAG ATCTGCGAGC TGGAGGCTAA TGGAGACTAT 
ATCCCAGCCG TAGTGGACCA CCGAGTAGGC ATGCCTTGCC ATGGCACATT CATGTTACAT 
CAGGGCATCC AAAGGAGAGT TACAGTCACC ATGGTACATG AGACAGGAAG AGACATTGAC 
TGGAAGGAAG TGCGAGAGCT GGTTGTAGGA CGTATCCGGA ACACGCCTGA AGCAGATGAG 
GCAATCATCG ACCCCAATAT TCTCTCTCTG AACATACTGT CTGCAGGATA CATTCGACCC 
GCGCATGATG ACAGGACATT CTACCGGTTT GAGGCTGCTT GGGATTCATC CATGCACAAC 
TCTTTGCTGC TAAACCGTGT GACTCCTTAC GGAGAGAAAA TCTACATGAC TCTGTCTGCT 
TACCTGGAGA TGGAGAACTG CACACAACCC ACAGTGATTA CCAAAGACTT ATGCATGGTG 
TTCCACTCAA GAGACGCCAA ACTTCCAGCA TCTCGCTCCA TCAGAAACCT CTTCAGCAGT 
GGCACCTTAA GGCCGTCTGA AGGAAACCGT GTAACCGGTG TGTATGAGCT CAGTTTATGC 
CACCTGGCTG ATGCAGGGAG CCCAGGAATG CAAAGGCGGC GCAGGAGGGT GCTGGACACG 
TCGGTGGCTT ATGTGCGAGG GGAGGAGAAC CTGGCTGGCT GGCGGCCCCG CAGTGACAGC 
CTCATCCTGG ACCATCAGTG GGAGCTGGAG AAATTGAGCC TCCTGCAGGA GGTGGAGAAA 
ACCAGACACT ACCTCCTCCT TAGGGAGAAG CTGGAGTCCA CCCTGCTGCT GGGCCAGGAG 
TCGCTGCTGT CCTGCGGGAG TGAGGATCTA ACCGAGTCCT CTTCCCCCTC CACTCACCTC 
GGCCTGGGCC ACAACCCCGG CCCCGGCCCC GACACACCCA ATGAGAGGCA GAGGGAGCTG 
GCTGCTAAGT GTCTGCGTTT GCTTAACCAC ACATTTAACA GGGACTACAG CCATGTGTGT 
GTTAGCGCCA GTGAGAGCAA GTTGAGTGAG ATGTCAGTGA CTCTCCTGAA AGACTCTGCT 
TCAGTGTGTG CCGAAAGCAC CCTCACACCC TCCTCCACAT GTCCTTCACT TGTGGAGAGT 
CGATACATTC ACAACACTGA TCTCAGACCC CCAACACCCC GATCTCGTGC TGTGAGCCCT 
AATCCTGACT CTGAAACAGA TGGTAAGAAG ATTCAAGAGC CCAGATCAAG AACCCGCACT 
TTTGTGCCAG ACATTCAGGA AATCCGTGTC AGCCCAATTG TGTCAAAGAA AGGCTATTTG 
CACTTTCTGG AGCCCCACAG TAATGGCTGG GTTAAGAGAT ATGTGGTGGT TCGTCGGCCT 
TATGTCTACA TCTACAACTC AGACAGAGAC ACAGTGGAAA GGGCCATTCT TAACCTGTCC 
TCAGCGCAAG TGGAGTATAG CGAAGACCAG CAGGCCATGC TAAAGACCCC CTACACATTT 
GCGGTATGCA CAGAGCATCG TGGGATACTC CTTCAAGCCA GTAATGACAA GGAGATGCAT 
GACTGGCTGT ATGCCTTTAA CCCTCTGCTT GCTGGATCTA TCAGGTCTAA ACTGTCCAGA 
AGGAGAGCTG GGCAGATGAG GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_005165955.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166012.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X17, mRNA.

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CloneID	ODa11882
Clone ID Related Accession (Same CDS sequence)	XM_005165955.3
Accession Version	XM_005165955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGCATT TCCTTGGGGA CTGATTCTAG GACATTCTAC 
CGGTTTGAGG CTGCTTGGGA TTCATCCATG CACAACTCTT TGCTGCTAAA CCGTGTGACT 
CCTTACGGAG AGAAAATCTA CATGACTCTG TCTGCTTACC TGGAGATGGA GAACTGCACA 
CAACCCACAG TGATTACCAA AGACTTATGC ATGGTGTTCC ACTCAAGAGA CGCCAAACTT 
CCAGCATCTC GCTCCATCAG AAACCTCTTC AGCAGTGGCA CCTTAAGGCC GTCTGAAGGA 
AACCGTGTAA CCGGTGTGTA TGAGCTCAGT TTATGCCACC TGGCTGATGC AGGGAGCCCA 
GGAATGCAAA GGCGGCGCAG GAGGGTGCTG GACACGTCGG TGGCTTATGT GCGAGGGGAG 
GAGAACCTGG CTGGCTGGCG GCCCCGCAGT GACAGCCTCA TCCTGGACCA TCAGTGGGAG 
CTGGAGAAAT TGAGCCTCCT GCAGGAGGTG GAGAAAACCA GACACTACCT CCTCCTTAGG 
GAGAAGCTGG AGTCCACCCT GCTGCTGGGC CAGGAGTCGC TGCTGTCCTG CGGGAGTGAG 
GATCTAACCG AGTCCTCTTC CCCCTCCACT CACCTCGGCC TGGGCCACAA CCCCGGCCCC 
GGCCCCGACA CACCCAATGA GAGGCAGAGG GAGCTGGCTG CTAAGTGTCT GCGTTTGCTT 
AACCACACAT TTAACAGGGA CTACAGCCAT GTGTGTGTTA GCGCCAGTGA GAGCAAGTTG 
AGTGAGATGT CAGTGACTCT CCTGAAAGAC TCTGCTTCAG TGTGTGCCGA AAGCACCCTC 
ACACCCTCCT CCACATGTCC TTCACTTGTG GAGAGTCGAT ACATTCACAA CACTGATCTC 
AGACCCCCAA CACCCCGATC TCGTGCTGTG AGCCCTAATC CTGACTCTGA AACAGATGGT 
AAGAAGATTC AAGAGCCCAG ATCAAGAACC CGCACTTTTG TGCCAGACAT TCAGGAAATC 
CGTGTCAGCC CAATTGTGTC AAAGAAAGGC TATTTGCACT TTCTGGAGCC CCACAGTAAT 
GGCTGGGTTA AGAGATATGT GGTGGTTCGT CGGCCTTATG TCTACATCTA CAACTCAGAC 
AGAGACACAG TGGAAAGGGC CATTCTTAAC CTGTCCTCAG CGCAAGTGGA GTATAGCGAA 
GACCAGCAGG CCATGCTAAA GACCCCCTAC ACATTTGCGG TATGCACAGA GCATCGTGGG 
ATACTCCTTC AAGCCAGTAA TGACAAGGAG ATGCATGACT GGCTGTATGC CTTTAACCCT 
CTGCTTGCTG GATCTATCAG GTCTAAACTG TCCAGAAGGA GAGCTGGGCA GATGAGGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166012.1
CDS	350..5392
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDSISLGTDSRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_005165955.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165955.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGCATT TCCTTGGGGA CTGATTCTAG GACATTCTAC 
CGGTTTGAGG CTGCTTGGGA TTCATCCATG CACAACTCTT TGCTGCTAAA CCGTGTGACT 
CCTTACGGAG AGAAAATCTA CATGACTCTG TCTGCTTACC TGGAGATGGA GAACTGCACA 
CAACCCACAG TGATTACCAA AGACTTATGC ATGGTGTTCC ACTCAAGAGA CGCCAAACTT 
CCAGCATCTC GCTCCATCAG AAACCTCTTC AGCAGTGGCA CCTTAAGGCC GTCTGAAGGA 
AACCGTGTAA CCGGTGTGTA TGAGCTCAGT TTATGCCACC TGGCTGATGC AGGGAGCCCA 
GGAATGCAAA GGCGGCGCAG GAGGGTGCTG GACACGTCGG TGGCTTATGT GCGAGGGGAG 
GAGAACCTGG CTGGCTGGCG GCCCCGCAGT GACAGCCTCA TCCTGGACCA TCAGTGGGAG 
CTGGAGAAAT TGAGCCTCCT GCAGGAGGTG GAGAAAACCA GACACTACCT CCTCCTTAGG 
GAGAAGCTGG AGTCCACCCT GCTGCTGGGC CAGGAGTCGC TGCTGTCCTG CGGGAGTGAG 
GATCTAACCG AGTCCTCTTC CCCCTCCACT CACCTCGGCC TGGGCCACAA CCCCGGCCCC 
GGCCCCGACA CACCCAATGA GAGGCAGAGG GAGCTGGCTG CTAAGTGTCT GCGTTTGCTT 
AACCACACAT TTAACAGGGA CTACAGCCAT GTGTGTGTTA GCGCCAGTGA GAGCAAGTTG 
AGTGAGATGT CAGTGACTCT CCTGAAAGAC TCTGCTTCAG TGTGTGCCGA AAGCACCCTC 
ACACCCTCCT CCACATGTCC TTCACTTGTG GAGAGTCGAT ACATTCACAA CACTGATCTC 
AGACCCCCAA CACCCCGATC TCGTGCTGTG AGCCCTAATC CTGACTCTGA AACAGATGGT 
AAGAAGATTC AAGAGCCCAG ATCAAGAACC CGCACTTTTG TGCCAGACAT TCAGGAAATC 
CGTGTCAGCC CAATTGTGTC AAAGAAAGGC TATTTGCACT TTCTGGAGCC CCACAGTAAT 
GGCTGGGTTA AGAGATATGT GGTGGTTCGT CGGCCTTATG TCTACATCTA CAACTCAGAC 
AGAGACACAG TGGAAAGGGC CATTCTTAAC CTGTCCTCAG CGCAAGTGGA GTATAGCGAA 
GACCAGCAGG CCATGCTAAA GACCCCCTAC ACATTTGCGG TATGCACAGA GCATCGTGGG 
ATACTCCTTC AAGCCAGTAA TGACAAGGAG ATGCATGACT GGCTGTATGC CTTTAACCCT 
CTGCTTGCTG GATCTATCAG GTCTAAACTG TCCAGAAGGA GAGCTGGGCA GATGAGGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_002662438.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002662484.2 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ODa11883
Clone ID Related Accession (Same CDS sequence)	XM_002662438.5
Accession Version	XM_002662438.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002662438.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGGACA TTCTACCGGT TTGAGGCTGC TTGGGATTCA 
TCCATGCACA ACTCTTTGCT GCTAAACCGT GTGACTCCTT ACGGAGAGAA AATCTACATG 
ACTCTGTCTG CTTACCTGGA GATGGAGAAC TGCACACAAC CCACAGTGAT TACCAAAGAC 
TTATGCATGG TGTTCCACTC AAGAGACGCC AAACTTCCAG CATCTCGCTC CATCAGAAAC 
CTCTTCAGCA GTGGCACCTT AAGGCCGTCT GAAGGAAACC GTGTAACCGG TGTGTATGAG 
CTCAGTTTAT GCCACCTGGC TGATGCAGGG AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG 
GTGCTGGACA CGTCGGTGGC TTATGTGCGA GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC 
CGCAGTGACA GCCTCATCCT GGACCATCAG TGGGAGCTGG AGAAATTGAG CCTCCTGCAG 
GAGGTGGAGA AAACCAGACA CTACCTCCTC CTTAGGGAGA AGCTGGAGTC CACCCTGCTG 
CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG AGTGAGGATC TAACCGAGTC CTCTTCCCCC 
TCCACTCACC TCGGCCTGGG CCACAACCCC GGCCCCGGCC CCGACACACC CAATGAGAGG 
CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT TTGCTTAACC ACACATTTAA CAGGGACTAC 
AGCCATGTGT GTGTTAGCGC CAGTGAGAGC AAGTTGAGTG AGATGTCAGT GACTCTCCTG 
AAAGACTCTG CTTCAGTGTG TGCCGAAAGC ACCCTCACAC CCTCCTCCAC ATGTCCTTCA 
CTTGTGGAGA GTCGATACAT TCACAACACT GATCTCAGAC CCCCAACACC CCGATCTCGT 
GCTGTGAGCC CTAATCCTGA CTCTGAAACA GATGGTAAGA AGATTCAAGA GCCCAGATCA 
AGAACCCGCA CTTTTGTGCC AGACATTCAG GAAATCCGTG TCAGCCCAAT TGTGTCAAAG 
AAAGGCTATT TGCACTTTCT GGAGCCCCAC AGTAATGGCT GGGTTAAGAG ATATGTGGTG 
GTTCGTCGGC CTTATGTCTA CATCTACAAC TCAGACAGAG ACACAGTGGA AAGGGCCATT 
CTTAACCTGT CCTCAGCGCA AGTGGAGTAT AGCGAAGACC AGCAGGCCAT GCTAAAGACC 
CCCTACACAT TTGCGGTATG CACAGAGCAT CGTGGGATAC TCCTTCAAGC CAGTAATGAC 
AAGGAGATGC ATGACTGGCT GTATGCCTTT AACCCTCTGC TTGCTGGATC TATCAGGTCT 
AAACTGTCCA GAAGGAGAGC TGGGCAGATG AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002662484.2
CDS	350..5368
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_002662438.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002662438.5

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGGACA TTCTACCGGT TTGAGGCTGC TTGGGATTCA 
TCCATGCACA ACTCTTTGCT GCTAAACCGT GTGACTCCTT ACGGAGAGAA AATCTACATG 
ACTCTGTCTG CTTACCTGGA GATGGAGAAC TGCACACAAC CCACAGTGAT TACCAAAGAC 
TTATGCATGG TGTTCCACTC AAGAGACGCC AAACTTCCAG CATCTCGCTC CATCAGAAAC 
CTCTTCAGCA GTGGCACCTT AAGGCCGTCT GAAGGAAACC GTGTAACCGG TGTGTATGAG 
CTCAGTTTAT GCCACCTGGC TGATGCAGGG AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG 
GTGCTGGACA CGTCGGTGGC TTATGTGCGA GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC 
CGCAGTGACA GCCTCATCCT GGACCATCAG TGGGAGCTGG AGAAATTGAG CCTCCTGCAG 
GAGGTGGAGA AAACCAGACA CTACCTCCTC CTTAGGGAGA AGCTGGAGTC CACCCTGCTG 
CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG AGTGAGGATC TAACCGAGTC CTCTTCCCCC 
TCCACTCACC TCGGCCTGGG CCACAACCCC GGCCCCGGCC CCGACACACC CAATGAGAGG 
CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT TTGCTTAACC ACACATTTAA CAGGGACTAC 
AGCCATGTGT GTGTTAGCGC CAGTGAGAGC AAGTTGAGTG AGATGTCAGT GACTCTCCTG 
AAAGACTCTG CTTCAGTGTG TGCCGAAAGC ACCCTCACAC CCTCCTCCAC ATGTCCTTCA 
CTTGTGGAGA GTCGATACAT TCACAACACT GATCTCAGAC CCCCAACACC CCGATCTCGT 
GCTGTGAGCC CTAATCCTGA CTCTGAAACA GATGGTAAGA AGATTCAAGA GCCCAGATCA 
AGAACCCGCA CTTTTGTGCC AGACATTCAG GAAATCCGTG TCAGCCCAAT TGTGTCAAAG 
AAAGGCTATT TGCACTTTCT GGAGCCCCAC AGTAATGGCT GGGTTAAGAG ATATGTGGTG 
GTTCGTCGGC CTTATGTCTA CATCTACAAC TCAGACAGAG ACACAGTGGA AAGGGCCATT 
CTTAACCTGT CCTCAGCGCA AGTGGAGTAT AGCGAAGACC AGCAGGCCAT GCTAAAGACC 
CCCTACACAT TTGCGGTATG CACAGAGCAT CGTGGGATAC TCCTTCAAGC CAGTAATGAC 
AAGGAGATGC ATGACTGGCT GTATGCCTTT AACCCTCTGC TTGCTGGATC TATCAGGTCT 
AAACTGTCCA GAAGGAGAGC TGGGCAGATG AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_009302425.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300700.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X16, mRNA.

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CloneID	ODa49318
Clone ID Related Accession (Same CDS sequence)	XM_009302425.2
Accession Version	XM_009302425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGACAG TTTCTTGATT CGGACATGCC TAGGACATTC 
TACCGGTTTG AGGCTGCTTG GGATTCATCC ATGCACAACT CTTTGCTGCT AAACCGTGTG 
ACTCCTTACG GAGAGAAAAT CTACATGACT CTGTCTGCTT ACCTGGAGAT GGAGAACTGC 
ACACAACCCA CAGTGATTAC CAAAGACTTA TGCATGGTGT TCCACTCAAG AGACGCCAAA 
CTTCCAGCAT CTCGCTCCAT CAGAAACCTC TTCAGCAGTG GCACCTTAAG GCCGTCTGAA 
GGAAACCGTG TAACCGGTGT GTATGAGCTC AGTTTATGCC ACCTGGCTGA TGCAGGGAGC 
CCAGGAATGC AAAGGCGGCG CAGGAGGGTG CTGGACACGT CGGTGGCTTA TGTGCGAGGG 
GAGGAGAACC TGGCTGGCTG GCGGCCCCGC AGTGACAGCC TCATCCTGGA CCATCAGTGG 
GAGCTGGAGA AATTGAGCCT CCTGCAGGAG GTGGAGAAAA CCAGACACTA CCTCCTCCTT 
AGGGAGAAGC TGGAGTCCAC CCTGCTGCTG GGCCAGGAGT CGCTGCTGTC CTGCGGGAGT 
GAGGATCTAA CCGAGTCCTC TTCCCCCTCC ACTCACCTCG GCCTGGGCCA CAACCCCGGC 
CCCGGCCCCG ACACACCCAA TGAGAGGCAG AGGGAGCTGG CTGCTAAGTG TCTGCGTTTG 
CTTAACCACA CATTTAACAG GGACTACAGC CATGTGTGTG TTAGCGCCAG TGAGAGCAAG 
TTGAGTGAGA TGTCAGTGAC TCTCCTGAAA GACTCTGCTT CAGTGTGTGC CGAAAGCACC 
CTCACACCCT CCTCCACATG TCCTTCACTT GTGGAGAGTC GATACATTCA CAACACTGAT 
CTCAGACCCC CAACACCCCG ATCTCGTGCT GTGAGCCCTA ATCCTGACTC TGAAACAGAT 
GGTAAGAAGA TTCAAGAGCC CAGATCAAGA ACCCGCACTT TTGTGCCAGA CATTCAGGAA 
ATCCGTGTCA GCCCAATTGT GTCAAAGAAA GGCTATTTGC ACTTTCTGGA GCCCCACAGT 
AATGGCTGGG TTAAGAGATA TGTGGTGGTT CGTCGGCCTT ATGTCTACAT CTACAACTCA 
GACAGAGACA CAGTGGAAAG GGCCATTCTT AACCTGTCCT CAGCGCAAGT GGAGTATAGC 
GAAGACCAGC AGGCCATGCT AAAGACCCCC TACACATTTG CGGTATGCAC AGAGCATCGT 
GGGATACTCC TTCAAGCCAG TAATGACAAG GAGATGCATG ACTGGCTGTA TGCCTTTAAC 
CCTCTGCTTG CTGGATCTAT CAGGTCTAAA CTGTCCAGAA GGAGAGCTGG GCAGATGAGG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300700.1
CDS	350..5395
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_009302425.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302425.2

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGG GCTTTTGTCT ATTTGAGTAA TCTGCTGTAT 
CCTGTGCCTC TGGTCCATCG GGTTGCCATC GTCAATGAGA AGGGAGAAGT CAAAGGTTTC 
CTCAGAGTAG CTGTACAGGC AATATCAGCC GATGAAGAGG CACCAGACTA TGGCTCTGGT 
GTCAGACAGT CAGGCACGGC CAAGATTTCT TTTGAGGACC AGCAGTTTGA GAAGTTCCAG 
TCAGAGTCAT GTCATTCTGG TCTTTCGCGT GCTGCCGTTT CTCAGGAAGA GTTGAGGATT 
GTGGAGGGTG AAGGACAAAG TGCAGAGATG GGACTGTCAG CAGATGAGGT CAACAATAAT 
ACATGTGCAG CTAACCCAGA GGAGCTGGAC AGCCCTGTCA AAACAGGTCT TGATGGGATG 
CTGGACGGCG CTCTGGATCA CTTGAGGATT GGTGAAGTCT TCACATTCAG AGTTACTGTT 
CTACAGGCGT CAAGCATTTC CGCAGAGTAC GCAGACATAT TCTGCCAGTT CAATTTTATT 
CACAGGCACG ATGAAGCATT TTCCACCGAG CCGTTGAAGA ACACTGGACG TGGTCCACCA 
CTTGGCTTCT ATCATGTGCA GAATATTGCA GTGGAAGTGA CTAAGTCCTT TATTGAGTAC 
ATAAAAACTC AGCCAATCGT GTTTGAAGTC TTTGGACACT ACCAGAAGCA GCCTTTCCCT 
GCGCTCTGCA AGGATCTCAT TAGCCCATTG CGTCCATGCA GACGACAGTT CCCAAGGGTC 
ATGCCTCTTT CCAAACCAGT CCCTGCTACC AAACTGACAG CCCTCACTCG ACCCACAGCA 
GGACCATGCC ACTGTAAATA TGACCTGATG GTCTTCTTTG AGATCTGCGA GCTGGAGGCT 
AATGGAGACT ATATCCCAGC CGTAGTGGAC CACCGAGTAG GCATGCCTTG CCATGGCACA 
TTCATGTTAC ATCAGGGCAT CCAAAGGAGA GTTACAGTCA CCATGGTACA TGAGACAGGA 
AGAGACATTG ACTGGAAGGA AGTGCGAGAG CTGGTTGTAG GACGTATCCG GAACACGCCT 
GAAGCAGATG AGGCAATCAT CGACCCCAAT ATTCTCTCTC TGAACATACT GTCTGCAGGA 
TACATTCGAC CCGCGCATGA TGACAGACAG TTTCTTGATT CGGACATGCC TAGGACATTC 
TACCGGTTTG AGGCTGCTTG GGATTCATCC ATGCACAACT CTTTGCTGCT AAACCGTGTG 
ACTCCTTACG GAGAGAAAAT CTACATGACT CTGTCTGCTT ACCTGGAGAT GGAGAACTGC 
ACACAACCCA CAGTGATTAC CAAAGACTTA TGCATGGTGT TCCACTCAAG AGACGCCAAA 
CTTCCAGCAT CTCGCTCCAT CAGAAACCTC TTCAGCAGTG GCACCTTAAG GCCGTCTGAA 
GGAAACCGTG TAACCGGTGT GTATGAGCTC AGTTTATGCC ACCTGGCTGA TGCAGGGAGC 
CCAGGAATGC AAAGGCGGCG CAGGAGGGTG CTGGACACGT CGGTGGCTTA TGTGCGAGGG 
GAGGAGAACC TGGCTGGCTG GCGGCCCCGC AGTGACAGCC TCATCCTGGA CCATCAGTGG 
GAGCTGGAGA AATTGAGCCT CCTGCAGGAG GTGGAGAAAA CCAGACACTA CCTCCTCCTT 
AGGGAGAAGC TGGAGTCCAC CCTGCTGCTG GGCCAGGAGT CGCTGCTGTC CTGCGGGAGT 
GAGGATCTAA CCGAGTCCTC TTCCCCCTCC ACTCACCTCG GCCTGGGCCA CAACCCCGGC 
CCCGGCCCCG ACACACCCAA TGAGAGGCAG AGGGAGCTGG CTGCTAAGTG TCTGCGTTTG 
CTTAACCACA CATTTAACAG GGACTACAGC CATGTGTGTG TTAGCGCCAG TGAGAGCAAG 
TTGAGTGAGA TGTCAGTGAC TCTCCTGAAA GACTCTGCTT CAGTGTGTGC CGAAAGCACC 
CTCACACCCT CCTCCACATG TCCTTCACTT GTGGAGAGTC GATACATTCA CAACACTGAT 
CTCAGACCCC CAACACCCCG ATCTCGTGCT GTGAGCCCTA ATCCTGACTC TGAAACAGAT 
GGTAAGAAGA TTCAAGAGCC CAGATCAAGA ACCCGCACTT TTGTGCCAGA CATTCAGGAA 
ATCCGTGTCA GCCCAATTGT GTCAAAGAAA GGCTATTTGC ACTTTCTGGA GCCCCACAGT 
AATGGCTGGG TTAAGAGATA TGTGGTGGTT CGTCGGCCTT ATGTCTACAT CTACAACTCA 
GACAGAGACA CAGTGGAAAG GGCCATTCTT AACCTGTCCT CAGCGCAAGT GGAGTATAGC 
GAAGACCAGC AGGCCATGCT AAAGACCCCC TACACATTTG CGGTATGCAC AGAGCATCGT 
GGGATACTCC TTCAAGCCAG TAATGACAAG GAGATGCATG ACTGGCTGTA TGCCTTTAAC 
CCTCTGCTTG CTGGATCTAT CAGGTCTAAA CTGTCCAGAA GGAGAGCTGG GCAGATGAGG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_009302426.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300701.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.

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CloneID	ODa49319
Clone ID Related Accession (Same CDS sequence)	XM_009302426.2 , XM_009302426.3
Accession Version	XM_009302426.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1A isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300701.1
CDS	350..5698
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_009302426.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302426.2

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1aa ( XM_009302426.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300701.1 Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X19, mRNA.

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CloneID	ODa49319
Clone ID Related Accession (Same CDS sequence)	XM_009302426.2 , XM_009302426.3
Accession Version	XM_009302426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300701.1
CDS	351..5699
Translation	MAGASVKVAVRVRPFNSREIGKESKCIIQMSGNTTTIINPKAPKENKSFNFDYSYWSHTSPEDINYASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPLLCEDLFTKINENNDNNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVMSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDSETDNTSEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDSGSNKNKKKKKVESFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLVRELKDEVSRLKDLLYAQGLGDIIEMTNAMTGMSPSPSLSALSSRAGSISSLHDRIMFSPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDASSRQDIVLSGHFIKEEHCTFTSTTGPMGEAVILEPCEGAETYVNGKRVTEPTVLKSGNRIILGKSHVFRFNHPEQARQERDRTPCAETPVEPVDWAFAQRELLDKQGIDMKQEMDQRLQELEDQYRKEREEANNLLEQQRLDYESRLEALQKQVDRCYPDAAEEDEEPEEEVQWTERETELAMWAFRKWRCYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDREKSPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSNAVEDCDSVLTGGDPFYDRFPWFRLVGRNPLFSTCLSEQASDPNSSPTPSEPTSTSELTELVQSWPDKKVGTELDDDGFLSDDPCSDLEEEGEDDDDDEVDDDEELCRSSGKAEDPFYDRSPLFSVVGRAFVYLSNLLYPVPLVHRVAIVNEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFEDQQFEKFQSESCHSGLSRAAVSQEELRIVEGEGQSAEMGLSADEVNNNTCAANPEELDSPVKTGLDGMLDGALDHLRIGEVFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKTQPIVFEVFGHYQKQPFPALCKDLISPLRPCRRQFPRVMPLSKPVPATKLTALTRPTAGPCHCKYDLMVFFEICELEANGDYIPAVVDHRVGMPCHGTFMLHQGIQRRVTVTMVHETGRDIDWKEVRELVVGRIRNTPEADEAIIDPNILSLNILSAGYIRPAHDDRQFLDSDMPRTFYRFEAAWDSSMHNSLLLNRVTPYGEKIYMTLSAYLEMENCTQPTVITKDLCMVFHSRDAKLPASRSIRNLFSSGTLRPSEGNRVTGVYELSLCHLADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLESTLLLGQESLLSCGSEDLTESSSPSTHLGLGHNPGPGPDTPNERQRELAAKCLRLLNHTFNRDYSHVCVSASESKLSEMSVTLLKDSASVCAESTLTPSSTCPSLVESRYIHNTDLRPPTPRSRAVSPNPDSETDGKKIQEPRSRTRTFVPDIQEIRVSPIVSKKGYLHFLEPHSNGWVKRYVVVRRPYVYIYNSDRDTVERAILNLSSAQVEYSEDQQAMLKTPYTFAVCTEHRGILLQASNDKEMHDWLYAFNPLLAGSIRSKLSRRRAGQMRF

Target ORF information:

RefSeq Version	XM_009302426.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1Aa (kif1aa), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302426.3

ORF Insert Sequence:

ATGGCAGGGG CCTCGGTGAA GGTGGCGGTG AGAGTTCGTC CCTTTAACTC TCGGGAGATC 
GGTAAAGAGT CTAAATGCAT CATCCAGATG TCCGGAAACA CAACCACCAT CATCAACCCC 
AAAGCACCCA AAGAAAACAA AAGCTTCAAT TTTGATTACT CCTACTGGTC ACACACTTCG 
CCTGAAGATA TCAACTATGC GTCCCAGCAG CAAGTCTACA GAGACATCGG AGAGGAGATG 
TTGCTCCATG CATTTGAAGG CTACAATGTC TGTATTTTTG CATACGGTCA AACTGGGGCC 
GGTAAATCCT ATACCATGAT GGGCAAACAG GAGAAGGACC AGCAGGGGAT CATTCCACTG 
CTGTGTGAAG ACCTTTTCAC TAAGATAAAT GAGAACAATG ACAACAACAT GTCCTATTCT 
GTGGAGGTTA GTTATATGGA GATCTACTGT GAGAGGGTAC GGGATCTTCT AAACCCTAAA 
AACAAAGGAA ACCTACGTGT GCGAGAACAT CCGTTGCTAG GCCCATATGT AGAAGATCTG 
TCCAAACTTG CCGTCATGTC ATATAATGAC ATCCAAGACC TGATGGACTC CGGGAATAAG 
GCTAGGACAG TAGCAGCCAC AAACATGAAT GAGACCAGCA GTCGTTCACA CGCTGTCTTT 
AACATCATCT TCACCCAGAG GCGGCACGAC TCGGAGACAG ACAACACTTC AGAAAAGGTC 
AGCAAAATCA GTCTGGTGGA TTTAGCGGGC AGTGAGAGAG CAGACTCTAC TGGAGCCAAA 
GGCACACGAC TCAAGGAAGG GGCAAACATC AATAAATCAC TCACCACCCT GGGAAAAGTC 
ATTTCAGCTC TGGCTGAGGT GGACTCTGGC TCTAACAAGA ACAAAAAGAA AAAGAAAGTG 
GAAAGTTTTA TTCCTTACAG GGATTCTGTG CTGACCTGGC TACTGAGAGA GAATCTCGGT 
GGCAACTCTC GAACCGCAAT GGTGGCTGCT CTCAGTCCAG CCGACATCAA CTATGATGAA 
ACCCTCAGTA CTCTCAGGTA TGCAGACAGG GCTAAGCAGA TCCGTTGTAA TGCAGTGATC 
AATGAAGATC CTAATAACCG TCTGGTGCGT GAGCTTAAAG ATGAAGTGTC CCGTCTGAAA 
GACCTCCTCT ATGCCCAGGG CCTGGGTGAC ATCATTGAGA TGACCAATGC TATGACTGGG 
ATGAGCCCCT CTCCATCACT CTCTGCCCTG TCCAGCCGGG CCGGTTCCAT TAGCAGCCTG 
CACGATCGCA TCATGTTCAG CCCCGGGAGC GAAGAAGCCA TTGAGAGACT CAAGGAAACT 
GAGAAAATCA TTGCAGAGCT CAATGAAACA TGGGAGGAGA AGCTTCGCCG TACAGAGGCC 
ATCAGGATGG ACAGAGAAGC ACTTCTGGCG GAGATGGGTG TTGCCATGCG TGAGGATGGA 
GGAACTCTTG GAGTCTTTTC CCCAAAAAAG ACTCCCCACC TAGTGAATCT CAATGAAGAT 
CCCTTGATGT CTGAATGTCT TCTATACTAC ATCAAAGATG GGATTACCAG GGTGGGCAGA 
GAAGATGCCA GTAGTCGGCA GGACATTGTT CTGAGCGGAC ACTTCATTAA GGAGGAACAT 
TGTACTTTCA CCAGCACCAC TGGACCAATG GGAGAAGCTG TCATTCTTGA GCCATGTGAG 
GGAGCAGAAA CATATGTAAA TGGCAAGAGA GTCACAGAAC CCACTGTTCT CAAATCAGGC 
AACCGAATCA TTTTGGGCAA GAGCCACGTG TTCCGCTTCA ACCATCCAGA ACAGGCCCGT 
CAGGAACGTG ACCGAACCCC CTGCGCTGAG ACCCCAGTGG AGCCGGTAGA CTGGGCCTTT 
GCTCAGAGAG AACTGCTGGA TAAACAGGGC ATTGACATGA AGCAGGAGAT GGATCAGAGA 
CTGCAGGAAC TTGAAGATCA GTATCGCAAG GAGAGAGAAG AGGCCAACAA TCTACTGGAG 
CAACAGAGAC TAGATTATGA GAGCAGGTTA GAGGCTCTGC AGAAACAGGT CGATCGATGT 
TATCCTGACG CAGCAGAGGA AGATGAAGAG CCTGAGGAGG AAGTGCAATG GACGGAGAGG 
GAGACGGAAC TGGCAATGTG GGCTTTCCGC AAATGGAGAT GTTATCAGTT TACATCCCTT 
CGAGATCTTC TTTGGGGCAA TGCCATCTTC CTTAAAGAGG CCAATGCCAT TAGTGTGGAG 
CTAAAGAAGA AGGTTCAGTT CCAGTTCGTG TTATTGACGG ACACACTTTA CTCTCCTCTT 
CCGCCTGACC TGTTACCGCC TGAAGCAGCT AAAGACAGAG AGAAAAGCCC ATTTCCACGC 
ACCATTGTAG CTGTTGAAGT TCAAGATCAG AAAAACGGAG CCACGCATTA CTGGACCCTG 
GAAAAGCTCA GGCAGAGACT GGATCTGATG AGAGAGATGT ATGATCGAGC TGCTGAGGTG 
CCCAGCAATG CTGTTGAAGA CTGTGACAGT GTGCTGACCG GAGGAGATCC TTTCTACGAC 
CGCTTTCCCT GGTTTCGTCT GGTTGGAAGA AATCCCCTTT TCTCCACATG CCTTAGTGAG 
CAAGCCAGCG ACCCCAACTC CTCCCCCACC CCCTCTGAAC CCACCTCCAC CTCTGAACTC 
ACCGAGCTCG TCCAGTCATG GCCAGACAAG AAGGTGGGGA CAGAGCTGGA TGACGATGGT 
TTCTTATCAG ACGACCCGTG CTCGGATTTA GAGGAGGAGG GGGAGGATGA TGATGATGAT 
GAGGTTGATG ATGACGAAGA GCTCTGCAGA AGCTCCGGCA AAGCTGAGGA CCCGTTTTAT 
GATCGTTCAC CATTGTTCAG TGTAGTAGGA AGGGCTTTTG TCTATTTGAG TAATCTGCTG 
TATCCTGTGC CTCTGGTCCA TCGGGTTGCC ATCGTCAATG AGAAGGGAGA AGTCAAAGGT 
TTCCTCAGAG TAGCTGTACA GGCAATATCA GCCGATGAAG AGGCACCAGA CTATGGCTCT 
GGTGTCAGAC AGTCAGGCAC GGCCAAGATT TCTTTTGAGG ACCAGCAGTT TGAGAAGTTC 
CAGTCAGAGT CATGTCATTC TGGTCTTTCG CGTGCTGCCG TTTCTCAGGA AGAGTTGAGG 
ATTGTGGAGG GTGAAGGACA AAGTGCAGAG ATGGGACTGT CAGCAGATGA GGTCAACAAT 
AATACATGTG CAGCTAACCC AGAGGAGCTG GACAGCCCTG TCAAAACAGG TCTTGATGGG 
ATGCTGGACG GCGCTCTGGA TCACTTGAGG ATTGGTGAAG TCTTCACATT CAGAGTTACT 
GTTCTACAGG CGTCAAGCAT TTCCGCAGAG TACGCAGACA TATTCTGCCA GTTCAATTTT 
ATTCACAGGC ACGATGAAGC ATTTTCCACC GAGCCGTTGA AGAACACTGG ACGTGGTCCA 
CCACTTGGCT TCTATCATGT GCAGAATATT GCAGTGGAAG TGACTAAGTC CTTTATTGAG 
TACATAAAAA CTCAGCCAAT CGTGTTTGAA GTCTTTGGAC ACTACCAGAA GCAGCCTTTC 
CCTGCGCTCT GCAAGGATCT CATTAGCCCA TTGCGTCCAT GCAGACGACA GTTCCCAAGG 
GTCATGCCTC TTTCCAAACC AGTCCCTGCT ACCAAACTGA CAGCCCTCAC TCGACCCACA 
GCAGGACCAT GCCACTGTAA ATATGACCTG ATGGTCTTCT TTGAGATCTG CGAGCTGGAG 
GCTAATGGAG ACTATATCCC AGCCGTAGTG GACCACCGAG TAGGCATGCC TTGCCATGGC 
ACATTCATGT TACATCAGGG CATCCAAAGG AGAGTTACAG TCACCATGGT ACATGAGACA 
GGAAGAGACA TTGACTGGAA GGAAGTGCGA GAGCTGGTTG TAGGACGTAT CCGGAACACG 
CCTGAAGCAG ATGAGGCAAT CATCGACCCC AATATTCTCT CTCTGAACAT ACTGTCTGCA 
GGATACATTC GACCCGCGCA TGATGACAGA CAGTTTCTTG ATTCGGACAT GCCTAGGACA 
TTCTACCGGT TTGAGGCTGC TTGGGATTCA TCCATGCACA ACTCTTTGCT GCTAAACCGT 
GTGACTCCTT ACGGAGAGAA AATCTACATG ACTCTGTCTG CTTACCTGGA GATGGAGAAC 
TGCACACAAC CCACAGTGAT TACCAAAGAC TTATGCATGG TGTTCCACTC AAGAGACGCC 
AAACTTCCAG CATCTCGCTC CATCAGAAAC CTCTTCAGCA GTGGCACCTT AAGGCCGTCT 
GAAGGAAACC GTGTAACCGG TGTGTATGAG CTCAGTTTAT GCCACCTGGC TGATGCAGGG 
AGCCCAGGAA TGCAAAGGCG GCGCAGGAGG GTGCTGGACA CGTCGGTGGC TTATGTGCGA 
GGGGAGGAGA ACCTGGCTGG CTGGCGGCCC CGCAGTGACA GCCTCATCCT GGACCATCAG 
TGGGAGCTGG AGAAATTGAG CCTCCTGCAG GAGGTGGAGA AAACCAGACA CTACCTCCTC 
CTTAGGGAGA AGCTGGAGTC CACCCTGCTG CTGGGCCAGG AGTCGCTGCT GTCCTGCGGG 
AGTGAGGATC TAACCGAGTC CTCTTCCCCC TCCACTCACC TCGGCCTGGG CCACAACCCC 
GGCCCCGGCC CCGACACACC CAATGAGAGG CAGAGGGAGC TGGCTGCTAA GTGTCTGCGT 
TTGCTTAACC ACACATTTAA CAGGGACTAC AGCCATGTGT GTGTTAGCGC CAGTGAGAGC 
AAGTTGAGTG AGATGTCAGT GACTCTCCTG AAAGACTCTG CTTCAGTGTG TGCCGAAAGC 
ACCCTCACAC CCTCCTCCAC ATGTCCTTCA CTTGTGGAGA GTCGATACAT TCACAACACT 
GATCTCAGAC CCCCAACACC CCGATCTCGT GCTGTGAGCC CTAATCCTGA CTCTGAAACA 
GATGGTAAGA AGATTCAAGA GCCCAGATCA AGAACCCGCA CTTTTGTGCC AGACATTCAG 
GAAATCCGTG TCAGCCCAAT TGTGTCAAAG AAAGGCTATT TGCACTTTCT GGAGCCCCAC 
AGTAATGGCT GGGTTAAGAG ATATGTGGTG GTTCGTCGGC CTTATGTCTA CATCTACAAC 
TCAGACAGAG ACACAGTGGA AAGGGCCATT CTTAACCTGT CCTCAGCGCA AGTGGAGTAT 
AGCGAAGACC AGCAGGCCAT GCTAAAGACC CCCTACACAT TTGCGGTATG CACAGAGCAT 
CGTGGGATAC TCCTTCAAGC CAGTAATGAC AAGGAGATGC ATGACTGGCT GTATGCCTTT 
AACCCTCTGC TTGCTGGATC TATCAGGTCT AAACTGTCCA GAAGGAGAGC TGGGCAGATG 
AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kif1aa
Entrez Gene ID	100330443
Full Name	kinesin family member 1Aa
Synonyms	kif1a
General protein information	Preferred Names kinesin family member 1Aa Names kinesin-like protein KIF1A kinesin family member 1A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	KIF1A	XP_002799156.1
Gallus gallus (chicken)	KIF1A	XP_003641781.1
Rattus norvegicus (Norway rat)	Kif1a	XP_006245623.1
Drosophila melanogaster (fruit fly)	unc-104	NP_001246373.1
Anopheles gambiae (African malaria mosquito)	KIF1A_ANOGA	XP_314493.3
Pan troglodytes (chimpanzee)	KIF1A	XP_003309612.1
Bos taurus (cattle)	KIF1A	XP_005205116.1
Mus musculus (house mouse)	Kif1a	NP_032466.2
Caenorhabditis elegans (roundworm)	unc-104	NP_001022041.1
Homo sapiens (human)	KIF1A	NP_004312.2
Canis lupus familiaris (dog)	KIF1A	NP_001161945.1
Danio rerio (zebrafish)	kif1a	XP_005166002.1
Danio rerio (zebrafish)	kif1ab	XP_005171271.1
Xenopus tropicalis (tropical clawed frog)	kif1a	XP_002933380.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kif1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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