frem1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	frem1a
Entrez Gene ID	100216326
Full Name	Fras1 related extracellular matrix 1a
Synonyms	rfl
General protein information	Preferred Names Fras1 related extracellular matrix 1a Names FRAS1-related extracellular matrix protein 1 rafels
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190308.1	NP_001177237.1	FRAS1-related extracellular matrix protein 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Nagendran M, Arora P, Gori P, Mulay A, Ray S, Jacob T, Sonawane M
Development (Cambridge, England)142(2)320-30(2015 Jan)

AMACO is a component of the basement membrane-associated Fraser complex.
Richardson RJ, Gebauer JM, Zhang JL, Kobbe B, Keene DR, Karlsen KR, Richetti S, Wohl AP, Sengle G, Neiss WF, Paulsson M, Hammerschmidt M, Wagener R
The Journal of investigative dermatology134(5)1313-1322(2014 May)

Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes.
Carney TJ, Feitosa NM, Sonntag C, Slanchev K, Kluger J, Kiyozumi D, Gebauer JM, Coffin Talbot J, Kimmel CB, Sekiguchi K, Wagener R, Schwarz H, Ingham PW, Hammerschmidt M
PLoS genetics6(4)e1000907(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The fish gene complement includes fras1, frem1a, frem1b, frem2a, frem2b, and frem3, which display complex overlapping and complementary expression patterns in developing tissues including the pharyngeal arches, hypochord, musculature, and otic vesicle.
Title: Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis.

The following frem1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frem1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31903	NM_001190308.1 Latest version!	Danio rerio Fras1 related extracellular matrix 1a (frem1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frem1a ( NM_001190308.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001177237.1 Danio rerio Fras1 related extracellular matrix 1a (frem1a), mRNA.

Price & Availability↑

CloneID	ODa31903
Clone ID Related Accession (Same CDS sequence)	NM_001190308.1
Accession Version	NM_001190308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	FRAS1-related extracellular matrix protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU936659.1. On Jun 16, 2010 this sequence version replaced XM_002667396.1. ##Evidence-Data-START## Transcript exon combination :: GU936659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTAA AAACCAGCAA ATGTGTGTGT TTCTTCCTGC TGGGCCTGAT CCAGCTGATT 
CGAGGCTCTC TGGTCAAAAC CAACGCTGTC CTGAAGGTGA AAAGAGGTCA GTCGGTGTTT 
CTGCAGGAGG ACGATCTGCA GTTTCGTATT CCCAGAGTCA AGGATTCCTG CAAGCTGGAG 
GTGGTGCTGA ATGAACCGAT CACACAGCGG GTCGGCTCTC TGACGCCACA GGTTTTTGAC 
TGTCACTTTA TGGCTGATGA GGTGAAATAC ACTCATAATG GATGTCCAAT TTTAAAAGAG 
GACACTGTTA AACTTCGTCT TTACAGATTT ACAGAGACGG AGACGTACAG CGAGCTCTTC 
TCTCTCCGTG TTGAAATCCT GGAGCCTGAA TGTAATGTGA TTCAGTTCGG ATCACAGCAA 
CTCGTGGTGC CTGAGTTTTA CGGCCTCTCG AACGTCCTGG ATGGAAACGT CGTGTCCTTC 
AATTATGAAC ACAGACACAA TATTGAGTGC ACAATTCGGG TGACGGGTCT GGAGACGCAT 
TTACCTGTTC ACGGTCAGCT GGTGACTGGA GAACCAGAGA AAGCAGACGG GCCCAGAGGA 
GATGAACCCG ACAGCTTCGT TTCTCTTAGG AGACAGCTGG ATAATAAAGC CAGAGCTCAC 
TGTAAGACTG ATGACTGCCT GAAGGGTCTC AAACTGCTGA AAATCACCAA AGTGCCATGC 
AATGACTTCC TGATGATGGG CATCCGCTAC CAGCACACCG ACCCTCCGTC TCCACATACA 
GACTACATCT CCATCAGACT GGACCTCACA GACACCCGGA GCAGGAGCAT AATACAGTCA 
GAGCAGGCCT GGATCCCGGT GACCATTACA GGCGCTCTCC CCAACCAGCC GCCCAAACCT 
GCCTTCATGT CCATGTTCAT CCTGGAGGTG GATCAGTTCA TCCTGACTCC TCTCTCCACT 
GCGACTGTGG ACGCGGAGGA CGACGAGACG CCCAAGCAGA GGCTGGTGTT CAACATCACT 
AAACCCCCCA GTGAAGGCTT CATTACTCAC CTGTCGGACC ACACCAAACC AGTGTCCTCC 
TTCAGCTGGA CGGATCTGAA TGGCATGCTG ATCAGCTTCC AGCCTCCCAA CTCCAGCGCC 
ACACATCGCA GGAACTACGA GGTGGAGTTT GAAGTGCATG ATTTCTACTT TGAGAAAAGC 
CAGCCTGTGA CGGTTCACGT GTCCATCAGG ACAGCGGATA CCAATGCRCC AAGAGTCTCC 
TGGAATATGG GTCTCAGTCT GTTGGAAGGT CAGTCTCGCC CAATCACATG GGATCAGCTG 
CAGATTGTTG ACAACGACAA CCTGAAGGCT GTGAAGATCA TCACTGTGGA CGGCCTGCAG 
CACGGCAGAC TCACTGTTAG AGGTGGAAAA GGCTTCATGT TCACTGTGAA CGATATTAAA 
AGCGGCGTCG TGCATTACCA CCATGATGAC AGCGACACTA CTAAAGATTA TATAGTCTTC 
CGGATCACCG ACGGCTTACA TCAAACTAGA CACAAGTTTC CCATCAACAT CCTGCCCAAA 
GATGACAGTC CGCCGTTCCT CATTACCAAC ATGCTGCTGG AGCTTTCTGA AGGCCAGACG 
GCTCTGCTGA GAGGATCCAT CCTGCAGGCC TGCGACATGG ACTCCAGTGA CGACTACATC 
ATGTTCAACG TCACTAGAGC TCCACAGGCT GGAGAAATCA TGAAGATGCC AGGTCCTGGG 
ATCACTGGAT ACCCTGTGAA CCGTTTTCTT CAGAAAGACC TTTTCCATTC YATCATCTAT 
TATCGGCACT CAGGCAGAGA GGTGTTTGAY GACTCTTTTG AGGTGGTGCT GTCTGACTTT 
CATGACCCTC CTAACCTTTC AGAGCCTCAG GTCATTGTGA TTCAGATCCA GCCAGTGCCT 
GATCAGCCTC CACAAGAAGC TCCGGGTGTA TCGAGACACT TAGTCATCAA TGAGACTGAT 
GTTGTCTATT TGACCAAGCA ACAACTGCAC TTTGTAGATT TGGAGTCCCC AGATAGTGAG 
CTTACATACA CCGTCACCAC CCCACCTTTC TACACCACCA CCTATGGAGG GTCTGATGCA 
GGAAGGTTGT TTCTGGTCGA CAGCATCCCC AAATTTAGCA AAGACTCAAG CGCACCGATG 
CTGAGGCTTT TCACTCAGCA CGCAGTCAAC TACATGAAAG TAGCTTACAT GCCCCCCATC 
CCGGACATCG GACCGTACCC TCAGCACATC CAGTTTGTCC TGTCAGTGAC CAACCAGCAA 
GGCAGCACGA CCACCGGGAT CTGCTTTAAC GTAACTGTTC TGCCAGTGGA TAACCAGGCA 
CCAGAGGTGC AAGTGAAGCT GCTGGTAGTG GATGAAGGAG GTGAGATCTG GGTGACTGAA 
GACCACATGC ATCTCTCTGA CCGGGACTCA CTGCAGGACT CAATACACGT GGAGCTGAAG 
ACTGCACCTC AACATGGCAG TGTTTATCTG GACGGCAAAC TCATGGTTCC AGGCCAGACT 
TTCACTGTGA GGGATCTGAA AAGCCTAAAA GTGAGATATC GTCACGACAG TTCTGAGACA 
GAACGTGATG AAATTAAATT CATTGCTACG GATGGCACAA ATACAGCAGA TTTCATATTR 
CGCATCAAGG TGACGCTGGT CAACGACGAG GTGCCAGTTY TAGTGGCAGG ACTGAAACCC 
ATGTTGTGGT GTGCAGAGGG TCAAGAAGTG TTTATTACCA TCGAGTACAT CTGTGCCACA 
GATCCAGACA GCGATGATCG ATCTCTCATG TATATGATCG CTCGACAGCC GTATCATGGA 
GTCGTTCAGA GAAACGGCAT CATTGTAGAC CGCTTCATTC AAGCAGATCT AAACGCTGGT 
TTCATTTCAT ACAGACACAC AGGRCAAGAG ATTGGGCTTC GTCCACGTCA TGACGTCGTC 
ACCTTTGTGA TCTCTGATGG AGAAACGGAG CCAATTTCTC CATGYTGCAG TGAAAGAGAC 
ACATTATTCA ACCCACAAAA CTCTCTGCCT GTTTATGACC TTAATATCAC CGTCTATCCT 
GTCAACAACC AGCCACCATC GATTGCTACA GGTGAGGTGT TTATGGTGGA TGAGGGTGGA 
TCAGCCTCCA TCACTGTGAC CCATCTGACT RTTGTAGACC AGGACTCTGC GCCTGAGGAT 
CTCCAGCTCA TCCTGGTTTC TCCTCCACAC TTTGGCTACA TTGAAAACAT CCTGCCCAGT 
CCAGGATTTG AAAAGAGCAA CGTGGGAATC AGTATTGCCT CGTTCACCTA CAGGGATGTG 
TTGGATGGAC ACATTAACTA TGTCCAGTCC AGACACCAGA GGATGGAGCC CACCACTGAC 
CAGCTCATGC TGCAGGTGTC TGATGGGAAA CAGCAGTCAT CTCCAGTCCC GCTTTACATC 
ATCATCAGTC CAACTAATGA CGAGATTCCA GACTTCCAGA CTCAAAACAT CACGGTTTTT 
GAAGGAGACA GGACAGAGTT AAACTCGTCT GTAATCAGTG CGGTGGATTT GGATGTTCCC 
CAAGAGCGTC TGATGTTCAG CATCGTCCAG AATCCTCAGC ACGGCTCTAT CATCAGTCTG 
CCACACAGRA ATCAAGTCTC ACATTACAAG CGTGGAGCTG AGGAGCCTGT CCAGCATTTT 
TGCATGGATG ACCTCAAGAG CGGCATGACT CTGATGTATG TACACGATGA CTCTGAGAGT 
GAGCAGGATG AATTCATCCT TCAGCTCTCT GATGGAAAAC ACAAGCTCCA GAAACACGTG 
CAGGTGAAAA TACTGCCTGT CAAYGACGAG GAGCCGCAAA TCATCCGGAA CACTGGGATT 
GAGATGGAGG CTGGAGAGTC CAGGCTGATC TCCAGTGCTG TTCTCAGCGC TCATGATGAA 
GATACTCCTC CTGACCACAT CCTCTATGTG TTCGAGAGCT TACCGACTCA TGGCGCCATT 
CAGATGAAGG TGGGCTCAGA CTGGCTGTCT GTGTCTGCAG GAGTGAACTG CTCTCAGAAG 
GTGCTGGATA TGAATCTGTT GCGTTACCTT CACACCGCTC ATCCCGGAGA ACCCACTGCC 
GATTTCTTTG TCTTCCACCT GTTTGATGGG AAGAACCGCT CTCCTCCTCA GCACTTCCAC 
ATTACAGTCA AACAGCTAGA AAAAGGAGAT ATAGCGCTGT TCCTGAAGCC TGTGCGGGTC 
AGTCGTGGAG ATCGTGTGGT TTTTACTACA GACGTCCTGC TGKCTGTGGA TGGTGCTGAC 
CAGCCGGAGG AGCTGCTRTA TGTGATCACT GCACCTCCAG CCCATGGACA CGTGGAGCTY 
ATCAAACACA GCGGAGTGCA GATTCAGTCC TTCAGTCAGC TGGACATCGC AGCAAACCTG 
GTGTGTTACG TCCATGATAA CAGAGCCACG GCACCCAGAG AGAGCCTGCA GTTCATGGTC 
AGCAATGGGA TATCAACTCG CAACGGGACC CTGGAGATCT TAGTGGACCT GACGGATAAA 
GTTCTCCCCA CACTAGTGCA CAACAGCGGC CTGCGGGTTC CTCAGGGCTC CACGGTTGTT 
TTATCCACTG CTGTTTTATC CCTGTCTGAC CCCGACAGTC CAGCGTCCAC CCTGAGCTAC 
AGGCTGATTC AGACCCCTCA GTACGGACAG TTGCTCCTGA AGGGGCAGGC GCTGGTYGCT 
GGAGCTCATT TCACCCAGCA GGACCTGCAG GATCTGGACG TCACCTACAA ACACTCTGGA 
GGAGCTTCTC AGATAGACCG CTTCACTTTC ACAGCCTCAG ACAGCACAGA TAGAGGATTC 
CTGCTGGACG GGAGAGTGCA GACAGAGCCC GTCTTCTTCA CACTGCAGAT TGAAGCTCTG 
GACAGCTCCG CTCCACAGGT GGCAGTGCTG GAAACCCTGT GGAAAGTGGA GCTCCTGAAA 
GATGGCCGCT ACGGGATCTT CATTTCCTCC AGGGAGCTGA AGGCTGAAGA TACAAACACT 
GCAGATGCAG ATCTGACCTT TCACATCCTC AGAGCTCCAT ACATCGGCTA TCTGGAGAAC 
CACACCAGCG GTGAGTTTGT GAGGCAGCGT TTCTCTCAGA TGGATCTGAA CAGAAGGAAT 
ATTCTCTACG TCATAAACTC CGCTCTGGAC GCTTTATCTG ACAGTCTAGA GTTTGCCGTG 
TCTGACGCAC TGGGCAACAC CGGACTCTCT CACAAATTGA ACTTCAGCTG GGCCGGTGTT 
GAGCTGACCA GGACTCAGTA TACAGTATGT GAAAGTCAAG GCTCAGTTTC ACTAACCATC 
CAGAGGAAAG GGAACATGCA GGACTCCTCT TATGTGACCG TACAGGTGAA AGAGCTCACC 
GCATCTGCTG GGAAAGACTT TATACCAGCT TCATCTCTGA TTCAGTTTGA CCCAGGGGTG 
GCCAGTCGAG TTTGGAAAGT GGAGATTGTT CAGGACTCAT TGGAGGAAGC GGATGAGAAA 
TTTGAGGTTA GCTTGGCGTC TCCTGTAGCT GCGGTGCTGA AGGACAATTC RAGAGCCACA 
ATCTCCATTA AAGACTCAAA TGGGCAGTGT GTGGAGCGGC AGATCACAGC GGGCAGCAGT 
CTTCAGGGTC GTGAGGTTCG TCCAGGACCG TATCCTCAAC ACGGCTCCAT CCAGCTGGAG 
ACTTTACCSC TYTCTCCTGA GGGAGTCATC AGAGGTGATG GAGATACAAC AGATCAAGCT 
CCTGCTGCTC CCAAGAAAAG ACTCCGAGTC ACTGGGAATG GGAAAGTAGT TCAGCCTTCT 
GAAACCATCC GTCGTGGATC TGAGCTCATT TATAAATATC ATGGTTTGGT GTCGATGGCG 
GTGGAGGAGG ATGAATCGGC CTCCAGGACT GACAGACAGG CAGAGGTTCA GGTCAACAGC 
AGGGGGCAGC AGCAGCGTCT TCTTACTGTC CAGACTAAAC CACAGCAAAC CTCCAGGCAC 
ACCAGCAGCA GACTCCTGCA GCGCACACAC ACTCAGACGT CCTCCAAGCG CTGTTCTCCA 
GATCTCTCAG GTCTCCTGCA TTATAACCAG AGCTCTGGTC GGCTGTTTCG CTGTAATGGA 
GACTCATGGA GGCCCTGGAC CCCCACTAAT GAGACTGTGA AAGCGCAGAG ATGTCCTCAG 
GGATGGACGT ACCACAGTAA TAACTGTTAT TTACTGAGCT CAGAGCGTAA AGCCTCATGG 
AGTACTGCAG CGCGGGCCTG CAGAGAGAGG TTCAGTGGTC ATCTGGTCAG TGTTCAGTCT 
AAAGCACACA TGGCCTGGCT GTGGGACTTC AGTCAGAGGA AACCCTTTTG GATCGGWCTG 
CATGATGGAG AAAGCAGRGG CCGMTGGGAG TGGATGGACG GACAATCGGT CACGTACACC 
AACTGGAGAC GAAACCCGCC AAAAAACCAG AAGAAGAAGG AGACGAGGAG GTGCGTCCTG 
GTGTGGAAGA AGAGTCGATG GCAGATCCGA GACTGTCAGA GAGGAAAAGG ACACCGCTAT 
GTGTGTTACA CTCAACTCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177237.1
CDS	242..6745
Translation	MTVKTSKCVCFFLLGLIQLIRGSLVKTNAVLKVKRGQSVFLQEDDLQFRIPRVKDSCKLEVVLNEPITQRVGSLTPQVFDCHFMADEVKYTHNGCPILKEDTVKLRLYRFTETETYSELFSLRVEILEPECNVIQFGSQQLVVPEFYGLSNVLDGNVVSFNYEHRHNIECTIRVTGLETHLPVHGQLVTGEPEKADGPRGDEPDSFVSLRRQLDNKARAHCKTDDCLKGLKLLKITKVPCNDFLMMGIRYQHTDPPSPHTDYISIRLDLTDTRSRSIIQSEQAWIPVTITGALPNQPPKPAFMSMFILEVDQFILTPLSTATVDAEDDETPKQRLVFNITKPPSEGFITHLSDHTKPVSSFSWTDLNGMLISFQPPNSSATHRRNYEVEFEVHDFYFEKSQPVTVHVSIRTADTNnullPRVSWNMGLSLLEGQSRPITWDQLQIVDNDNLKAVKIITVDGLQHGRLTVRGGKGFMFTVNDIKSGVVHYHHDDSDTTKDYIVFRITDGLHQTRHKFPINILPKDDSPPFLITNMLLELSEGQTALLRGSILQACDMDSSDDYIMFNVTRAPQAGEIMKMPGPGITGYPVNRFLQKDLFHnullIIYYRHSGREVFnullDSFEVVLSDFHDPPNLSEPQVIVIQIQPVPDQPPQEAPGVSRHLVINETDVVYLTKQQLHFVDLESPDSELTYTVTTPPFYTTTYGGSDAGRLFLVDSIPKFSKDSSAPMLRLFTQHAVNYMKVAYMPPIPDIGPYPQHIQFVLSVTNQQGSTTTGICFNVTVLPVDNQAPEVQVKLLVVDEGGEIWVTEDHMHLSDRDSLQDSIHVELKTAPQHGSVYLDGKLMVPGQTFTVRDLKSLKVRYRHDSSETERDEIKFIATDGTNTADFInullRIKVTLVNDEVPVnullVAGLKPMLWCAEGQEVFITIEYICATDPDSDDRSLMYMIARQPYHGVVQRNGIIVDRFIQADLNAGFISYRHTnullQEIGLRPRHDVVTFVISDGETEPISPnullCSERDTLFNPQNSLPVYDLNITVYPVNNQPPSIATGEVFMVDEGGSASITVTHLTnullVDQDSAPEDLQLILVSPPHFGYIENILPSPGFEKSNVGISIASFTYRDVLDGHINYVQSRHQRMEPTTDQLMLQVSDGKQQSSPVPLYIIISPTNDEIPDFQTQNITVFEGDRTELNSSVISAVDLDVPQERLMFSIVQNPQHGSIISLPHnullNQVSHYKRGAEEPVQHFCMDDLKSGMTLMYVHDDSESEQDEFILQLSDGKHKLQKHVQVKILPVnullDEEPQIIRNTGIEMEAGESRLISSAVLSAHDEDTPPDHILYVFESLPTHGAIQMKVGSDWLSVSAGVNCSQKVLDMNLLRYLHTAHPGEPTADFFVFHLFDGKNRSPPQHFHITVKQLEKGDIALFLKPVRVSRGDRVVFTTDVLLnullVDGADQPEELnullYVITAPPAHGHVEnullIKHSGVQIQSFSQLDIAANLVCYVHDNRATAPRESLQFMVSNGISTRNGTLEILVDLTDKVLPTLVHNSGLRVPQGSTVVLSTAVLSLSDPDSPASTLSYRLIQTPQYGQLLLKGQALnullAGAHFTQQDLQDLDVTYKHSGGASQIDRFTFTASDSTDRGFLLDGRVQTEPVFFTLQIEALDSSAPQVAVLETLWKVELLKDGRYGIFISSRELKAEDTNTADADLTFHILRAPYIGYLENHTSGEFVRQRFSQMDLNRRNILYVINSALDALSDSLEFAVSDALGNTGLSHKLNFSWAGVELTRTQYTVCESQGSVSLTIQRKGNMQDSSYVTVQVKELTASAGKDFIPASSLIQFDPGVASRVWKVEIVQDSLEEADEKFEVSLASPVAAVLKDNnullRATISIKDSNGQCVERQITAGSSLQGREVRPGPYPQHGSIQLETLnullnullSPEGVIRGDGDTTDQAPAAPKKRLRVTGNGKVVQPSETIRRGSELIYKYHGLVSMAVEEDESASRTDRQAEVQVNSRGQQQRLLTVQTKPQQTSRHTSSRLLQRTHTQTSSKRCSPDLSGLLHYNQSSGRLFRCNGDSWRPWTPTNETVKAQRCPQGWTYHSNNCYLLSSERKASWSTAARACRERFSGHLVSVQSKAHMAWLWDFSQRKPFWInullLHDGESnullGnullWEWMDGQSVTYTNWRRNPPKNQKKKETRRCVLVWKKSRWQIRDCQRGKGHRYVCYTQLH

Target ORF information:

RefSeq Version	NM_001190308.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Fras1 related extracellular matrix 1a (frem1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190308.1

ORF Insert Sequence:

ATGACTGTAA AAACCAGCAA ATGTGTGTGT TTCTTCCTGC TGGGCCTGAT CCAGCTGATT 
CGAGGCTCTC TGGTCAAAAC CAACGCTGTC CTGAAGGTGA AAAGAGGTCA GTCGGTGTTT 
CTGCAGGAGG ACGATCTGCA GTTTCGTATT CCCAGAGTCA AGGATTCCTG CAAGCTGGAG 
GTGGTGCTGA ATGAACCGAT CACACAGCGG GTCGGCTCTC TGACGCCACA GGTTTTTGAC 
TGTCACTTTA TGGCTGATGA GGTGAAATAC ACTCATAATG GATGTCCAAT TTTAAAAGAG 
GACACTGTTA AACTTCGTCT TTACAGATTT ACAGAGACGG AGACGTACAG CGAGCTCTTC 
TCTCTCCGTG TTGAAATCCT GGAGCCTGAA TGTAATGTGA TTCAGTTCGG ATCACAGCAA 
CTCGTGGTGC CTGAGTTTTA CGGCCTCTCG AACGTCCTGG ATGGAAACGT CGTGTCCTTC 
AATTATGAAC ACAGACACAA TATTGAGTGC ACAATTCGGG TGACGGGTCT GGAGACGCAT 
TTACCTGTTC ACGGTCAGCT GGTGACTGGA GAACCAGAGA AAGCAGACGG GCCCAGAGGA 
GATGAACCCG ACAGCTTCGT TTCTCTTAGG AGACAGCTGG ATAATAAAGC CAGAGCTCAC 
TGTAAGACTG ATGACTGCCT GAAGGGTCTC AAACTGCTGA AAATCACCAA AGTGCCATGC 
AATGACTTCC TGATGATGGG CATCCGCTAC CAGCACACCG ACCCTCCGTC TCCACATACA 
GACTACATCT CCATCAGACT GGACCTCACA GACACCCGGA GCAGGAGCAT AATACAGTCA 
GAGCAGGCCT GGATCCCGGT GACCATTACA GGCGCTCTCC CCAACCAGCC GCCCAAACCT 
GCCTTCATGT CCATGTTCAT CCTGGAGGTG GATCAGTTCA TCCTGACTCC TCTCTCCACT 
GCGACTGTGG ACGCGGAGGA CGACGAGACG CCCAAGCAGA GGCTGGTGTT CAACATCACT 
AAACCCCCCA GTGAAGGCTT CATTACTCAC CTGTCGGACC ACACCAAACC AGTGTCCTCC 
TTCAGCTGGA CGGATCTGAA TGGCATGCTG ATCAGCTTCC AGCCTCCCAA CTCCAGCGCC 
ACACATCGCA GGAACTACGA GGTGGAGTTT GAAGTGCATG ATTTCTACTT TGAGAAAAGC 
CAGCCTGTGA CGGTTCACGT GTCCATCAGG ACAGCGGATA CCAATGCRCC AAGAGTCTCC 
TGGAATATGG GTCTCAGTCT GTTGGAAGGT CAGTCTCGCC CAATCACATG GGATCAGCTG 
CAGATTGTTG ACAACGACAA CCTGAAGGCT GTGAAGATCA TCACTGTGGA CGGCCTGCAG 
CACGGCAGAC TCACTGTTAG AGGTGGAAAA GGCTTCATGT TCACTGTGAA CGATATTAAA 
AGCGGCGTCG TGCATTACCA CCATGATGAC AGCGACACTA CTAAAGATTA TATAGTCTTC 
CGGATCACCG ACGGCTTACA TCAAACTAGA CACAAGTTTC CCATCAACAT CCTGCCCAAA 
GATGACAGTC CGCCGTTCCT CATTACCAAC ATGCTGCTGG AGCTTTCTGA AGGCCAGACG 
GCTCTGCTGA GAGGATCCAT CCTGCAGGCC TGCGACATGG ACTCCAGTGA CGACTACATC 
ATGTTCAACG TCACTAGAGC TCCACAGGCT GGAGAAATCA TGAAGATGCC AGGTCCTGGG 
ATCACTGGAT ACCCTGTGAA CCGTTTTCTT CAGAAAGACC TTTTCCATTC YATCATCTAT 
TATCGGCACT CAGGCAGAGA GGTGTTTGAY GACTCTTTTG AGGTGGTGCT GTCTGACTTT 
CATGACCCTC CTAACCTTTC AGAGCCTCAG GTCATTGTGA TTCAGATCCA GCCAGTGCCT 
GATCAGCCTC CACAAGAAGC TCCGGGTGTA TCGAGACACT TAGTCATCAA TGAGACTGAT 
GTTGTCTATT TGACCAAGCA ACAACTGCAC TTTGTAGATT TGGAGTCCCC AGATAGTGAG 
CTTACATACA CCGTCACCAC CCCACCTTTC TACACCACCA CCTATGGAGG GTCTGATGCA 
GGAAGGTTGT TTCTGGTCGA CAGCATCCCC AAATTTAGCA AAGACTCAAG CGCACCGATG 
CTGAGGCTTT TCACTCAGCA CGCAGTCAAC TACATGAAAG TAGCTTACAT GCCCCCCATC 
CCGGACATCG GACCGTACCC TCAGCACATC CAGTTTGTCC TGTCAGTGAC CAACCAGCAA 
GGCAGCACGA CCACCGGGAT CTGCTTTAAC GTAACTGTTC TGCCAGTGGA TAACCAGGCA 
CCAGAGGTGC AAGTGAAGCT GCTGGTAGTG GATGAAGGAG GTGAGATCTG GGTGACTGAA 
GACCACATGC ATCTCTCTGA CCGGGACTCA CTGCAGGACT CAATACACGT GGAGCTGAAG 
ACTGCACCTC AACATGGCAG TGTTTATCTG GACGGCAAAC TCATGGTTCC AGGCCAGACT 
TTCACTGTGA GGGATCTGAA AAGCCTAAAA GTGAGATATC GTCACGACAG TTCTGAGACA 
GAACGTGATG AAATTAAATT CATTGCTACG GATGGCACAA ATACAGCAGA TTTCATATTR 
CGCATCAAGG TGACGCTGGT CAACGACGAG GTGCCAGTTY TAGTGGCAGG ACTGAAACCC 
ATGTTGTGGT GTGCAGAGGG TCAAGAAGTG TTTATTACCA TCGAGTACAT CTGTGCCACA 
GATCCAGACA GCGATGATCG ATCTCTCATG TATATGATCG CTCGACAGCC GTATCATGGA 
GTCGTTCAGA GAAACGGCAT CATTGTAGAC CGCTTCATTC AAGCAGATCT AAACGCTGGT 
TTCATTTCAT ACAGACACAC AGGRCAAGAG ATTGGGCTTC GTCCACGTCA TGACGTCGTC 
ACCTTTGTGA TCTCTGATGG AGAAACGGAG CCAATTTCTC CATGYTGCAG TGAAAGAGAC 
ACATTATTCA ACCCACAAAA CTCTCTGCCT GTTTATGACC TTAATATCAC CGTCTATCCT 
GTCAACAACC AGCCACCATC GATTGCTACA GGTGAGGTGT TTATGGTGGA TGAGGGTGGA 
TCAGCCTCCA TCACTGTGAC CCATCTGACT RTTGTAGACC AGGACTCTGC GCCTGAGGAT 
CTCCAGCTCA TCCTGGTTTC TCCTCCACAC TTTGGCTACA TTGAAAACAT CCTGCCCAGT 
CCAGGATTTG AAAAGAGCAA CGTGGGAATC AGTATTGCCT CGTTCACCTA CAGGGATGTG 
TTGGATGGAC ACATTAACTA TGTCCAGTCC AGACACCAGA GGATGGAGCC CACCACTGAC 
CAGCTCATGC TGCAGGTGTC TGATGGGAAA CAGCAGTCAT CTCCAGTCCC GCTTTACATC 
ATCATCAGTC CAACTAATGA CGAGATTCCA GACTTCCAGA CTCAAAACAT CACGGTTTTT 
GAAGGAGACA GGACAGAGTT AAACTCGTCT GTAATCAGTG CGGTGGATTT GGATGTTCCC 
CAAGAGCGTC TGATGTTCAG CATCGTCCAG AATCCTCAGC ACGGCTCTAT CATCAGTCTG 
CCACACAGRA ATCAAGTCTC ACATTACAAG CGTGGAGCTG AGGAGCCTGT CCAGCATTTT 
TGCATGGATG ACCTCAAGAG CGGCATGACT CTGATGTATG TACACGATGA CTCTGAGAGT 
GAGCAGGATG AATTCATCCT TCAGCTCTCT GATGGAAAAC ACAAGCTCCA GAAACACGTG 
CAGGTGAAAA TACTGCCTGT CAAYGACGAG GAGCCGCAAA TCATCCGGAA CACTGGGATT 
GAGATGGAGG CTGGAGAGTC CAGGCTGATC TCCAGTGCTG TTCTCAGCGC TCATGATGAA 
GATACTCCTC CTGACCACAT CCTCTATGTG TTCGAGAGCT TACCGACTCA TGGCGCCATT 
CAGATGAAGG TGGGCTCAGA CTGGCTGTCT GTGTCTGCAG GAGTGAACTG CTCTCAGAAG 
GTGCTGGATA TGAATCTGTT GCGTTACCTT CACACCGCTC ATCCCGGAGA ACCCACTGCC 
GATTTCTTTG TCTTCCACCT GTTTGATGGG AAGAACCGCT CTCCTCCTCA GCACTTCCAC 
ATTACAGTCA AACAGCTAGA AAAAGGAGAT ATAGCGCTGT TCCTGAAGCC TGTGCGGGTC 
AGTCGTGGAG ATCGTGTGGT TTTTACTACA GACGTCCTGC TGKCTGTGGA TGGTGCTGAC 
CAGCCGGAGG AGCTGCTRTA TGTGATCACT GCACCTCCAG CCCATGGACA CGTGGAGCTY 
ATCAAACACA GCGGAGTGCA GATTCAGTCC TTCAGTCAGC TGGACATCGC AGCAAACCTG 
GTGTGTTACG TCCATGATAA CAGAGCCACG GCACCCAGAG AGAGCCTGCA GTTCATGGTC 
AGCAATGGGA TATCAACTCG CAACGGGACC CTGGAGATCT TAGTGGACCT GACGGATAAA 
GTTCTCCCCA CACTAGTGCA CAACAGCGGC CTGCGGGTTC CTCAGGGCTC CACGGTTGTT 
TTATCCACTG CTGTTTTATC CCTGTCTGAC CCCGACAGTC CAGCGTCCAC CCTGAGCTAC 
AGGCTGATTC AGACCCCTCA GTACGGACAG TTGCTCCTGA AGGGGCAGGC GCTGGTYGCT 
GGAGCTCATT TCACCCAGCA GGACCTGCAG GATCTGGACG TCACCTACAA ACACTCTGGA 
GGAGCTTCTC AGATAGACCG CTTCACTTTC ACAGCCTCAG ACAGCACAGA TAGAGGATTC 
CTGCTGGACG GGAGAGTGCA GACAGAGCCC GTCTTCTTCA CACTGCAGAT TGAAGCTCTG 
GACAGCTCCG CTCCACAGGT GGCAGTGCTG GAAACCCTGT GGAAAGTGGA GCTCCTGAAA 
GATGGCCGCT ACGGGATCTT CATTTCCTCC AGGGAGCTGA AGGCTGAAGA TACAAACACT 
GCAGATGCAG ATCTGACCTT TCACATCCTC AGAGCTCCAT ACATCGGCTA TCTGGAGAAC 
CACACCAGCG GTGAGTTTGT GAGGCAGCGT TTCTCTCAGA TGGATCTGAA CAGAAGGAAT 
ATTCTCTACG TCATAAACTC CGCTCTGGAC GCTTTATCTG ACAGTCTAGA GTTTGCCGTG 
TCTGACGCAC TGGGCAACAC CGGACTCTCT CACAAATTGA ACTTCAGCTG GGCCGGTGTT 
GAGCTGACCA GGACTCAGTA TACAGTATGT GAAAGTCAAG GCTCAGTTTC ACTAACCATC 
CAGAGGAAAG GGAACATGCA GGACTCCTCT TATGTGACCG TACAGGTGAA AGAGCTCACC 
GCATCTGCTG GGAAAGACTT TATACCAGCT TCATCTCTGA TTCAGTTTGA CCCAGGGGTG 
GCCAGTCGAG TTTGGAAAGT GGAGATTGTT CAGGACTCAT TGGAGGAAGC GGATGAGAAA 
TTTGAGGTTA GCTTGGCGTC TCCTGTAGCT GCGGTGCTGA AGGACAATTC RAGAGCCACA 
ATCTCCATTA AAGACTCAAA TGGGCAGTGT GTGGAGCGGC AGATCACAGC GGGCAGCAGT 
CTTCAGGGTC GTGAGGTTCG TCCAGGACCG TATCCTCAAC ACGGCTCCAT CCAGCTGGAG 
ACTTTACCSC TYTCTCCTGA GGGAGTCATC AGAGGTGATG GAGATACAAC AGATCAAGCT 
CCTGCTGCTC CCAAGAAAAG ACTCCGAGTC ACTGGGAATG GGAAAGTAGT TCAGCCTTCT 
GAAACCATCC GTCGTGGATC TGAGCTCATT TATAAATATC ATGGTTTGGT GTCGATGGCG 
GTGGAGGAGG ATGAATCGGC CTCCAGGACT GACAGACAGG CAGAGGTTCA GGTCAACAGC 
AGGGGGCAGC AGCAGCGTCT TCTTACTGTC CAGACTAAAC CACAGCAAAC CTCCAGGCAC 
ACCAGCAGCA GACTCCTGCA GCGCACACAC ACTCAGACGT CCTCCAAGCG CTGTTCTCCA 
GATCTCTCAG GTCTCCTGCA TTATAACCAG AGCTCTGGTC GGCTGTTTCG CTGTAATGGA 
GACTCATGGA GGCCCTGGAC CCCCACTAAT GAGACTGTGA AAGCGCAGAG ATGTCCTCAG 
GGATGGACGT ACCACAGTAA TAACTGTTAT TTACTGAGCT CAGAGCGTAA AGCCTCATGG 
AGTACTGCAG CGCGGGCCTG CAGAGAGAGG TTCAGTGGTC ATCTGGTCAG TGTTCAGTCT 
AAAGCACACA TGGCCTGGCT GTGGGACTTC AGTCAGAGGA AACCCTTTTG GATCGGWCTG 
CATGATGGAG AAAGCAGRGG CCGMTGGGAG TGGATGGACG GACAATCGGT CACGTACACC 
AACTGGAGAC GAAACCCGCC AAAAAACCAG AAGAAGAAGG AGACGAGGAG GTGCGTCCTG 
GTGTGGAAGA AGAGTCGATG GCAGATCCGA GACTGTCAGA GAGGAAAAGG ACACCGCTAT 
GTGTGTTACA CTCAACTCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Development (Cambridge, England)142(2)320-30(2015 Jan)
Nagendran M,Arora P,Gori P,Mulay A,Ray S,Jacob T,Sonawane M

AMACO is a component of the basement membrane-associated Fraser complex.
The Journal of investigative dermatology134(5)1313-1322(2014 May)
Richardson RJ,Gebauer JM,Zhang JL,Kobbe B,Keene DR,Karlsen KR,Richetti S,Wohl AP,Sengle G,Neiss WF,Paulsson M,Hammerschmidt M,Wagener R

Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes.
PLoS genetics6(4)e1000907(2010 Apr)
Carney TJ,Feitosa NM,Sonntag C,Slanchev K,Kluger J,Kiyozumi D,Gebauer JM,Coffin Talbot J,Kimmel CB,Sekiguchi K,Wagener R,Schwarz H,Ingham PW,Hammerschmidt M

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frem1a cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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