tnfsf18 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnfsf18
Entrez Gene ID	100148577
Full Name	TNF superfamily member 18
General protein information	Preferred Names TNF superfamily member 18 Names TNF superfamily member 18 gitrl tumor necrosis factor (ligand) superfamily, member 18
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128818.1	NP_001122290.1	TNF superfamily member 18

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Biswas G, Kinoshita S, Kono T, Hikima J, Sakai M
Marine genomics2225-36(2015 Aug)

A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development.
Poulton LD, Nolan KF, Anastasaki C, Waldmann H, Patton EE
The International journal of developmental biology54(5)815-25(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest knock-down of gitrl, but not of gitr, resulted in disruption of early embryogenesis, most clearly revealed during gastrulation, which corresponded to the earliest peak in gitrl mRNA expression (5.25-10 hpf).
Title: A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development.

The following tnfsf18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnfsf18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04958	NM_001128818.1 Latest version!	Danio rerio TNF superfamily member 18 (tnfsf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnfsf18 ( NM_001128818.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122290.1 Danio rerio TNF superfamily member 18 (tnfsf18), mRNA.

Price & Availability↑

CloneID	ODa04958
Clone ID Related Accession (Same CDS sequence)	NM_001128818.1
Accession Version	NM_001128818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-02
Organism	Danio rerio(zebrafish)
Product	TNF superfamily member 18
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU099310.1. On Jul 31, 2008 this sequence version replaced XM_001923074.1. ##Evidence-Data-START## Transcript exon combination :: EU099310.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTGT CTGCCGAACA CTGCCGTGAT ACAAGTGGAG ACAGGGGTGG TGGTGGTGGT 
TTTAATGGTG CTTTGATCCA TCAGAGAACG CTGATTCGGG GACTGATCAT ATGGGTCACG 
CTACTCACTC TTGGCCTGGC AGCTTCAATA TCTCTGCATT TCATCCCTAA AGAATCGCCG 
GCTCCAAGTA AACAGCAGGG TTCGGAAACA ACCAGTAATA ATGTTTATCC TGGAAAGAAA 
ATAGATCTAA TGACATTCAC GCCAAACTGG GAAAACACAG ATGTTGAGCT CCTGCAGTGG 
AATACAGTTG AAGAAAGGTT CATCAAAGGA GAGGGACAGC TGACTGTGAC TCAGGGAGGC 
GCTTATTTTC TGTATCTCCA AGTTACCCTT GACTCGAGGA AAAAAGAAAA CCACACAATC 
ACAGTCCAAA CACAAAAACA AAATGTGATC TTAAAAGGTT ACATCAATGG ATCAAACCTC 
TCAAGTGGCT TCTTGGCTAC TGGAATTTAC CTGGATGATG ATGATACTTT TAATGTGACC 
TGCAAACCCA AAGCCAAAAT CCAGAACTCT CACATTGAAA CCTACATGGG TGTCATCAAG 
CTTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122290.1
CDS	14..622
Translation	MSLSAEHCRDTSGDRGGGGGFNGALIHQRTLIRGLIIWVTLLTLGLAASISLHFIPKESPAPSKQQGSETTSNNVYPGKKIDLMTFTPNWENTDVELLQWNTVEERFIKGEGQLTVTQGGAYFLYLQVTLDSRKKENHTITVQTQKQNVILKGYINGSNLSSGFLATGIYLDDDDTFNVTCKPKAKIQNSHIETYMGVIKLG

Target ORF information:

RefSeq Version	NM_001128818.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TNF superfamily member 18 (tnfsf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128818.1

ORF Insert Sequence:

ATGTCTCTGT CTGCCGAACA CTGCCGTGAT ACAAGTGGAG ACAGGGGTGG TGGTGGTGGT 
TTTAATGGTG CTTTGATCCA TCAGAGAACG CTGATTCGGG GACTGATCAT ATGGGTCACG 
CTACTCACTC TTGGCCTGGC AGCTTCAATA TCTCTGCATT TCATCCCTAA AGAATCGCCG 
GCTCCAAGTA AACAGCAGGG TTCGGAAACA ACCAGTAATA ATGTTTATCC TGGAAAGAAA 
ATAGATCTAA TGACATTCAC GCCAAACTGG GAAAACACAG ATGTTGAGCT CCTGCAGTGG 
AATACAGTTG AAGAAAGGTT CATCAAAGGA GAGGGACAGC TGACTGTGAC TCAGGGAGGC 
GCTTATTTTC TGTATCTCCA AGTTACCCTT GACTCGAGGA AAAAAGAAAA CCACACAATC 
ACAGTCCAAA CACAAAAACA AAATGTGATC TTAAAAGGTT ACATCAATGG ATCAAACCTC 
TCAAGTGGCT TCTTGGCTAC TGGAATTTAC CTGGATGATG ATGATACTTT TAATGTGACC 
TGCAAACCCA AAGCCAAAAT CCAGAACTCT CACATTGAAA CCTACATGGG TGTCATCAAG 
CTTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Marine genomics2225-36(2015 Aug)
Biswas G,Kinoshita S,Kono T,Hikima J,Sakai M

A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development.
The International journal of developmental biology54(5)815-25(2010)
Poulton LD,Nolan KF,Anastasaki C,Waldmann H,Patton EE