chia.5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chia.5
Entrez Gene ID	100003900
Full Name	chitinase, acidic.5
Synonyms	zgc:173927
General protein information	Preferred Names chitinase, acidic.5 Names uncharacterized protein LOC100003900 chioIIb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110041.3	NP_001103511.2	uncharacterized protein LOC100003900 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:65788	NP_955897.2
Danio rerio (zebrafish)	chia.4	NP_956740.1
Magnaporthe oryzae (rice blast fungus)	MGG_01247	XP_003714154.1
Bos taurus (cattle)	CHIA	NP_777124.1
Gallus gallus (chicken)	CHIA	NP_989760.1
Danio rerio (zebrafish)	chia.3	NP_998378.1
Homo sapiens (human)	CHIA	NP_970615.2
Xenopus tropicalis (tropical clawed frog)	chia	NP_001186489.1
	CHIA	XP_001104487.1
Canis lupus familiaris (dog)	CHIA	XP_537030.3
Mus musculus (house mouse)	Chia1	NP_075675.2
Rattus norvegicus (Norway rat)	Chia	NP_997469.1
Danio rerio (zebrafish)	zgc:173927	NP_001103511.1
Neurospora crassa	NCU03209	XP_965309.2

Gene Ontology
Bibliography

Related articles in PubMed

Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos.
Koch BE, Stougaard J, Spaink HP
Gene expression patterns : GEP14(2)69-77(2014 Mar)

New paralogues and revised time line in the expansion of the vertebrate GH18 family.
Hussain M, Wilson JB
Journal of molecular evolution76(4)240-60(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chia.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chia.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa47857	NM_001110041.3 Latest version!	Danio rerio chitinase, acidic.5 (chia.5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chia.5 ( NM_001110041.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103511.2 Danio rerio chitinase, acidic.5 (chia.5), mRNA.

Price & Availability↑

CloneID	ODa47857
Clone ID Related Accession (Same CDS sequence)	NM_001110041.3
Accession Version	NM_001110041.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100003900 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU234171.6. On Jun 18, 2015 this sequence version replaced NM_001110041.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments of non-reference strain transcripts. ##Evidence-Data-START## Transcript exon combination :: BC153462.1, GDQH01003421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476821, SAMN06350657 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGAT TCATTTTTAT AGCCAGCTTT TCACTGGCAT TGTGTCACTT TGGATTGGCC 
TCCCAACTTA TATGTTACTT CACCAACTGG TCTCAGTACA GACCTGATGT TGGCAAGTAC 
ATGCCATCCA ATGTGGATCC ACATCTGTGC ACTCATTTAA TTTATGCCTT TTCCATTATT 
AATAATGAAA ATAAACTGAC AACCTACGAA TGGAATGATG AAACTCTCTA CCAGTCCTTT 
AATGGCCTAA AACAAAGTAA CTCCAACCTG AAAACTCTGT TGGCTGTTGG TGGATGGGAA 
TTCGGCTCAG CTCAGTTTAG CAGCATGGTC TCCATGCCAC AAAACAGACA GACCTTTATT 
CAGTCCAGTA TCACATTTCT GAGGACCCAT GGATTTGACG GTCTGGATCT GGACTGGGAG 
TATCCTGGTT CTCGTGGAAG TCCTCCAGAA GACAAGCAGA GATTCACTTT GCTCTGTAAG 
GAGCTTGTAG AAGCTTATCA GGCAGAGAGT GCAGCCACTG GACGCCCCAG ACTCATGCTC 
ACTGCTGCAG TGGCTGCTGT GAAAGGAATC ATTGACGCTG GTTATGAAAT TGCAGAAATT 
GCCAAGTATT TGGACTTCAT CAATATCATG ACCTATGATT TCCACGGCTC ACAGGACAAC 
ATCACTGGAC ACAATAGTCC TCTGTACAGA GACTCTGGAG ACACAGGAGA CCAAATCTAC 
TACAATACTG ACTTTGCAAT GAACTACTGG CGAGATCAGG GCGCTCCTGT GGAGAAGCTC 
AGGATGGGCT TTGCTGCGTA TGGAAGAACG TTTCGCCTGT CGTCTGCGGA CAATGGAGTT 
GGAGCTCCAG CTAGTGGTGC TGCTTTAGCT GGAACATACA CCAGAGAAGC TGGATTCTGG 
TCCTATTATG AGATCTGTAC TTTTCTACAA GGAGCCATTG TGCAACAGAT TGTTGATCAG 
AAAGTTCCTT ATGCAACAAA AGGACAGGAG TGGGTGGGAT TTGATGACCA GGAAAGCTAT 
GAGACTAAGG TGGACTACCT GAAGGAGAAA GGCTTTGGAG GAGCTTTTGT CTGGTCTCTG 
GATCTTGATG ACTTCAGTGG TCAGTTCTGT GGGCAGGGCA AATACCCTCT TATTGGACAC 
CTCCACACGC TGCTTAATAT CAGCAACACT GATTTCCCAT CTCTCCCCCC AAGCACAACA 
GATAAGGCCA GTGGGACCTC TACAACAGCA TCTGCTGCTG TACCTCATAC CACAATACCT 
CCTGTTGTAC CGACAGCTCC CTCAGGATCA GACTTCTGTG CAAGCAGATC AGAGGGCATC 
TATGCGAATC CAGCTGATCC CAAAACATTC ATTCAGTGTG CCAATGGCAG GACCTTCATA 
CAGAATTGTC CTGCAACAAC AGTTTTTGAT CCTGACTGCA CATGCTGTAA CTGGCCTAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103511.2
CDS	44..1489
Translation	MSRFIFIASFSLALCHFGLASQLICYFTNWSQYRPDVGKYMPSNVDPHLCTHLIYAFSIINNENKLTTYEWNDETLYQSFNGLKQSNSNLKTLLAVGGWEFGSAQFSSMVSMPQNRQTFIQSSITFLRTHGFDGLDLDWEYPGSRGSPPEDKQRFTLLCKELVEAYQAESAATGRPRLMLTAAVAAVKGIIDAGYEIAEIAKYLDFINIMTYDFHGSQDNITGHNSPLYRDSGDTGDQIYYNTDFAMNYWRDQGAPVEKLRMGFAAYGRTFRLSSADNGVGAPASGAALAGTYTREAGFWSYYEICTFLQGAIVQQIVDQKVPYATKGQEWVGFDDQESYETKVDYLKEKGFGGAFVWSLDLDDFSGQFCGQGKYPLIGHLHTLLNISNTDFPSLPPSTTDKASGTSTTASAAVPHTTIPPVVPTAPSGSDFCASRSEGIYANPADPKTFIQCANGRTFIQNCPATTVFDPDCTCCNWPKK

Target ORF information:

RefSeq Version	NM_001110041.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chitinase, acidic.5 (chia.5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110041.3

ORF Insert Sequence:

ATGAGCAGAT TCATTTTTAT AGCCAGCTTT TCACTGGCAT TGTGTCACTT TGGATTGGCC 
TCCCAACTTA TATGTTACTT CACCAACTGG TCTCAGTACA GACCTGATGT TGGCAAGTAC 
ATGCCATCCA ATGTGGATCC ACATCTGTGC ACTCATTTAA TTTATGCCTT TTCCATTATT 
AATAATGAAA ATAAACTGAC AACCTACGAA TGGAATGATG AAACTCTCTA CCAGTCCTTT 
AATGGCCTAA AACAAAGTAA CTCCAACCTG AAAACTCTGT TGGCTGTTGG TGGATGGGAA 
TTCGGCTCAG CTCAGTTTAG CAGCATGGTC TCCATGCCAC AAAACAGACA GACCTTTATT 
CAGTCCAGTA TCACATTTCT GAGGACCCAT GGATTTGACG GTCTGGATCT GGACTGGGAG 
TATCCTGGTT CTCGTGGAAG TCCTCCAGAA GACAAGCAGA GATTCACTTT GCTCTGTAAG 
GAGCTTGTAG AAGCTTATCA GGCAGAGAGT GCAGCCACTG GACGCCCCAG ACTCATGCTC 
ACTGCTGCAG TGGCTGCTGT GAAAGGAATC ATTGACGCTG GTTATGAAAT TGCAGAAATT 
GCCAAGTATT TGGACTTCAT CAATATCATG ACCTATGATT TCCACGGCTC ACAGGACAAC 
ATCACTGGAC ACAATAGTCC TCTGTACAGA GACTCTGGAG ACACAGGAGA CCAAATCTAC 
TACAATACTG ACTTTGCAAT GAACTACTGG CGAGATCAGG GCGCTCCTGT GGAGAAGCTC 
AGGATGGGCT TTGCTGCGTA TGGAAGAACG TTTCGCCTGT CGTCTGCGGA CAATGGAGTT 
GGAGCTCCAG CTAGTGGTGC TGCTTTAGCT GGAACATACA CCAGAGAAGC TGGATTCTGG 
TCCTATTATG AGATCTGTAC TTTTCTACAA GGAGCCATTG TGCAACAGAT TGTTGATCAG 
AAAGTTCCTT ATGCAACAAA AGGACAGGAG TGGGTGGGAT TTGATGACCA GGAAAGCTAT 
GAGACTAAGG TGGACTACCT GAAGGAGAAA GGCTTTGGAG GAGCTTTTGT CTGGTCTCTG 
GATCTTGATG ACTTCAGTGG TCAGTTCTGT GGGCAGGGCA AATACCCTCT TATTGGACAC 
CTCCACACGC TGCTTAATAT CAGCAACACT GATTTCCCAT CTCTCCCCCC AAGCACAACA 
GATAAGGCCA GTGGGACCTC TACAACAGCA TCTGCTGCTG TACCTCATAC CACAATACCT 
CCTGTTGTAC CGACAGCTCC CTCAGGATCA GACTTCTGTG CAAGCAGATC AGAGGGCATC 
TATGCGAATC CAGCTGATCC CAAAACATTC ATTCAGTGTG CCAATGGCAG GACCTTCATA 
CAGAATTGTC CTGCAACAAC AGTTTTTGAT CCTGACTGCA CATGCTGTAA CTGGCCTAAG 
AAATAA