SI:CH211-248G20.5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-248G20.5
Entrez Gene ID	100002227
Full Name	si:ch211-248g20.5
General protein information	Preferred Names si:ch211-248g20.5 Names rho guanine nucleotide exchange factor 17 Rho guanine nucleotide exchange factor (GEF) 17
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003200414.5	XP_003200462.1	rho guanine nucleotide exchange factor 17
XM_003200414.4	XP_003200462.1	rho guanine nucleotide exchange factor 17

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGEF17	XP_003952045.1
Danio rerio (zebrafish)	si:ch211-248g20.5	XP_003200462.1
Homo sapiens (human)	ARHGEF17	NP_055601.2
	ARHGEF17	XP_001115376.1
Rattus norvegicus (Norway rat)	Arhgef17	XP_003748980.1
Gallus gallus (chicken)	ARHGEF17	XP_003640675.2
Xenopus tropicalis (tropical clawed frog)	arhgef17	XP_002943175.2
Canis lupus familiaris (dog)	ARHGEF17	XP_005633593.1
Mus musculus (house mouse)	Arhgef17	NP_001074585.1
Bos taurus (cattle)	ARHGEF17	XP_005201010.1

Gene Ontology

The following SI:CH211-248G20.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-248G20.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23018	XM_003200414.5 Latest version!	Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa23018	XM_003200414.4	Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-248G20.5 ( XM_003200414.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003200462.1 Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.

Price & Availability↑

CloneID	ODa23018
Clone ID Related Accession (Same CDS sequence)	XM_003200414.5 , XM_003200414.4
Accession Version	XM_003200414.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200414.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGA TGGTACGGAC CCTCGCCCAG TTCACCATCA CCCTGGAGGA GATGCAGGAG 
AGCGGAGAGA GCATCAGTGA TGATGGTGGA GGTGTGGGTG AAGAGGTGGA GCGCTCCGTC 
ACTGCCGAGC CCAATGGAAA CGGCATTGTG GGAAACACTG GAGCTCCTCA GACCATTGCT 
GAGGATATGA GAAGACATGT GATGATGAAT CTGCTGGACG CTGAACAGTC TTACGTGGAG 
TCTCTGCGCA CACTCATTCA GGGCTACATG AAGCCGCTGA AGCATCCAGA AAACCCGCCT 
TTGTGTGACC CGTCACTTGT GGACGAGATG TTTTACCAGA TCCCAGAAAT CCTGGAGCAC 
CATGAGCAGT TTCTGGAGCA GGTGCTGGAC TGTGTTCACC AGTGGCACGA CAAGCAGACC 
ATCGGACACG TGCTCATTCA GTCGTTTTCC AAAGAGACTC TGGCTAATAT TTATTCTGCA 
TACATCGACA ATTTCATGAA CGCCAAAGAC GCTGTGAGAA TCGCCAAGGA GGCCAAACCT 
GCTTTCCTCA AGTTTCTGGA GCAAAGCATG CGTGAGAACA GAGAGAAACA AGCTCTGGGT 
GACCTGATGA TAAAGCCGGT TCAGAGAATC CCTCGATACG AGCTGCTGGT CAAGGATCTG 
CTGAAACACA CTCCTGAGGA TCATCCAGAT TACCTGTTTC TGTTGGATGC TCAGAAGAAC 
ATCAAGCGTT TGGCGGAGCG GATCAACAAG GGCAGACGCA GTGCAGAGGA GCTGGAGAGA 
GAAACACGCG TCATGCAGGA GATCGAGGCG CACATCGAGG GTGTAGAACA TATCCTAAAC 
CCACAGAGAA AGTTCCTGAG ACAAGAGATG GTGGTGGAAG CGAAAACGGT GGGCGGGAAG 
AAGGACCGCT CTCTGTTTCT GTTCAGCGAT CTGCTGATCT GCACCACACT CAAGAGGAAA 
TCTGGCTCTC TGCGCAGGAG CTCCATGAGT TTATATTCTG CTGCTAGTGT CATCGACACC 
TCCAGTAAAT ACAAGTTCCT GTGGAAACTG CCTTTAGAAG ACATAGAGGT GGTGAAAGGT 
TCTAATCAAG CCACTAACAG AGAAAATGTC CAGAAAACCA TCAGTCGTCT GGATGAAGAT 
CTCAGCACAC TTGGACAAGT CAGCAAACTG TCAGAAACAC TCAGTTTCCC CCATCAGAGT 
TTGGACGATG TGATTAAGGA TCTGATGGCG TCGGTTCACC GTGAGCTGGC CGAGAAGCAG 
TCTCTGGCCT TCAGCATGAC TTTACTGCCC ACTAAACTGG AGCTGAGCTG CACTTCAGCC 
GAGAGCAGCT TCATCTTTGA GTTCAGCAGT CCGGACACTC GCAGCAACTT CGAGCAGGCC 
TTCGAGGAGG CCAAGAAGAA ACTCGCCATG AATAAGGACC AGTGGGACCC GGAGTTCCTG 
AAGGCCATCC CGATCATGAA GACGCGCAGC GGGATGCAGT TTTCCTGTGC GTCTCCCAGC 
CACAGCTGCC CGGAGAACAC GTATGAGGTG TGGGTGTGTA ACAGCGATGG TTACGTTGGT 
CAGGTGTGCC TGCTGAACAT CCGGGACGAG CCCACGGTGG AGGCCTGCAT CGCCGTCTGC 
TCCGCTCGCA TCATCTGCAT CGCCACTGTT CCGGGACTCA AGAACAGAGA GCGTGTGGTT 
CACTCCACAC CGACGGCTCC ATCAGATTCA AACTCTGCAG CGACAACTCA ACCACAGCTC 
CACATCTGCA TCTCTGGCTC GTCTCTGGAG CTCAGCGAGC CGCCAGCGGT GCCCACTGCA 
GAACTCGTGC CCTTCGACAG CGACGACACT GACGATGAGG ACTCGCCCAG CCCTTCATCA 
ACACTGCAAA GCCAGGCCTC ACACTCCACC ATATCATCCA GCTTCGGCAA TGATGAAGCG 
GTGGTGGCGA AGGACATGGC CACAGAGACC ACCAGCTCAG AGGAGGAGCA GGAGTTCCCA 
GTGCCCAGCT CTTTTGGAGC CCCTCGCCTC AGTGCTGAGA GCCCCATGGA TGGACGAGCC 
ATGCGCCGCT CCAGTCGAGG CTCCTTCACC CGCGCCAGCC TGGAGGACCT GCTGAGCATC 
GACCCCGAGG CCTACCAGAG CTCCGTGTGG CTCGGCACCG AGGATGGATG CATCCATGTT 
TACCAGTCAT CAGACAACAT CAGAAACCGG AAGAACAGTA TGAAGATGCA ACACTCGGCT 
TCAGTCCTTT GTATCCTGTA TCTTGACAAT AAAGTGTTTG TGTCTCTGGC AAACGGAGAG 
GTTATTGTGT ATCAGCGGGA GGCAGGGAGT TTCTGGGATC CTCAGAGCTC TCAGACTCTG 
TGTCTCGGGT CTCCAAGCGG ACCTGTGACT AAAATGGTTC CTGTGGCGGG AAAACTGTGG 
TGTGGCTGTC AGAACCGCAT CCTTATCATC AACACCAGCA CTTTAACACA AGAGCACTGG 
TTTCAGGTGG GTCAGGACAG CGGGCGCTGC GTGACCAGTA TGGTGTGTTT TGGTAAAGGT 
GTCTGGATCG CTCTGCAGGG CAGCGCTCAG GTGCGTCTCT ATCACTCCTC CACATACGAG 
AGTCTCACTG AGGTCGACGT CGCTCCAGCC GTTCACAAGA TGCTGGCAGG CTCTGATGCT 
ATTATACGTC AGCACAAGGC GGCGTGTTTG CGGATCACAG CTCTTCTGGC CTGTAAGGAT 
CTGCTGTGGA TCGGCACCAG TGCAGGGGTG GTGTTGACTC TGGTGGTCCC GCCGGTGTCC 
TCCAGCACAG GATCTGGATC TCTGCGTGTC CCGCTGATCC CCATGGGCTC TGCTCACGGA 
CACACTGGAC ATGTGCGCTT TCTGACCTGC ATCGAGCTGC CAGAGGGATT CGACGTGTCT 
TTTCCTCCGC CAGCAGAGTC AGCATCACAG AACAGCTCAG ACAGCGGCAC GGGAGGCCTA 
CAGAGGCGAG ATTCAGCCAG ACGACGCGCC TCCACCCTCC TCCCCACCAA ATCCAACCTG 
CTGGTCATCA GCGGCGGCGA TGGCTATGAA GACTTCAGAC TGACCAATAG CAGCGAGACG 
GTGGGGCGGG ACGACAGCAC CAACCACCTA TTGCTGTGGC GTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200462.1
CDS	390..3497
Translation	MLQMVRTLAQFTITLEEMQESGESISDDGGGVGEEVERSVTAEPNGNGIVGNTGAPQTIAEDMRRHVMMNLLDAEQSYVESLRTLIQGYMKPLKHPENPPLCDPSLVDEMFYQIPEILEHHEQFLEQVLDCVHQWHDKQTIGHVLIQSFSKETLANIYSAYIDNFMNAKDAVRIAKEAKPAFLKFLEQSMRENREKQALGDLMIKPVQRIPRYELLVKDLLKHTPEDHPDYLFLLDAQKNIKRLAERINKGRRSAEELERETRVMQEIEAHIEGVEHILNPQRKFLRQEMVVEAKTVGGKKDRSLFLFSDLLICTTLKRKSGSLRRSSMSLYSAASVIDTSSKYKFLWKLPLEDIEVVKGSNQATNRENVQKTISRLDEDLSTLGQVSKLSETLSFPHQSLDDVIKDLMASVHRELAEKQSLAFSMTLLPTKLELSCTSAESSFIFEFSSPDTRSNFEQAFEEAKKKLAMNKDQWDPEFLKAIPIMKTRSGMQFSCASPSHSCPENTYEVWVCNSDGYVGQVCLLNIRDEPTVEACIAVCSARIICIATVPGLKNRERVVHSTPTAPSDSNSAATTQPQLHICISGSSLELSEPPAVPTAELVPFDSDDTDDEDSPSPSSTLQSQASHSTISSSFGNDEAVVAKDMATETTSSEEEQEFPVPSSFGAPRLSAESPMDGRAMRRSSRGSFTRASLEDLLSIDPEAYQSSVWLGTEDGCIHVYQSSDNIRNRKNSMKMQHSASVLCILYLDNKVFVSLANGEVIVYQREAGSFWDPQSSQTLCLGSPSGPVTKMVPVAGKLWCGCQNRILIINTSTLTQEHWFQVGQDSGRCVTSMVCFGKGVWIALQGSAQVRLYHSSTYESLTEVDVAPAVHKMLAGSDAIIRQHKAACLRITALLACKDLLWIGTSAGVVLTLVVPPVSSSTGSGSLRVPLIPMGSAHGHTGHVRFLTCIELPEGFDVSFPPPAESASQNSSDSGTGGLQRRDSARRRASTLLPTKSNLLVISGGDGYEDFRLTNSSETVGRDDSTNHLLLWRV

Target ORF information:

RefSeq Version	XM_003200414.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200414.5

ORF Insert Sequence:

ATGCTGCAGA TGGTACGGAC CCTCGCCCAG TTCACCATCA CCCTGGAGGA GATGCAGGAG 
AGCGGAGAGA GCATCAGTGA TGATGGTGGA GGTGTGGGTG AAGAGGTGGA GCGCTCCGTC 
ACTGCCGAGC CCAATGGAAA CGGCATTGTG GGAAACACTG GAGCTCCTCA GACCATTGCT 
GAGGATATGA GAAGACATGT GATGATGAAT CTGCTGGACG CTGAACAGTC TTACGTGGAG 
TCTCTGCGCA CACTCATTCA GGGCTACATG AAGCCGCTGA AGCATCCAGA AAACCCGCCT 
TTGTGTGACC CGTCACTTGT GGACGAGATG TTTTACCAGA TCCCAGAAAT CCTGGAGCAC 
CATGAGCAGT TTCTGGAGCA GGTGCTGGAC TGTGTTCACC AGTGGCACGA CAAGCAGACC 
ATCGGACACG TGCTCATTCA GTCGTTTTCC AAAGAGACTC TGGCTAATAT TTATTCTGCA 
TACATCGACA ATTTCATGAA CGCCAAAGAC GCTGTGAGAA TCGCCAAGGA GGCCAAACCT 
GCTTTCCTCA AGTTTCTGGA GCAAAGCATG CGTGAGAACA GAGAGAAACA AGCTCTGGGT 
GACCTGATGA TAAAGCCGGT TCAGAGAATC CCTCGATACG AGCTGCTGGT CAAGGATCTG 
CTGAAACACA CTCCTGAGGA TCATCCAGAT TACCTGTTTC TGTTGGATGC TCAGAAGAAC 
ATCAAGCGTT TGGCGGAGCG GATCAACAAG GGCAGACGCA GTGCAGAGGA GCTGGAGAGA 
GAAACACGCG TCATGCAGGA GATCGAGGCG CACATCGAGG GTGTAGAACA TATCCTAAAC 
CCACAGAGAA AGTTCCTGAG ACAAGAGATG GTGGTGGAAG CGAAAACGGT GGGCGGGAAG 
AAGGACCGCT CTCTGTTTCT GTTCAGCGAT CTGCTGATCT GCACCACACT CAAGAGGAAA 
TCTGGCTCTC TGCGCAGGAG CTCCATGAGT TTATATTCTG CTGCTAGTGT CATCGACACC 
TCCAGTAAAT ACAAGTTCCT GTGGAAACTG CCTTTAGAAG ACATAGAGGT GGTGAAAGGT 
TCTAATCAAG CCACTAACAG AGAAAATGTC CAGAAAACCA TCAGTCGTCT GGATGAAGAT 
CTCAGCACAC TTGGACAAGT CAGCAAACTG TCAGAAACAC TCAGTTTCCC CCATCAGAGT 
TTGGACGATG TGATTAAGGA TCTGATGGCG TCGGTTCACC GTGAGCTGGC CGAGAAGCAG 
TCTCTGGCCT TCAGCATGAC TTTACTGCCC ACTAAACTGG AGCTGAGCTG CACTTCAGCC 
GAGAGCAGCT TCATCTTTGA GTTCAGCAGT CCGGACACTC GCAGCAACTT CGAGCAGGCC 
TTCGAGGAGG CCAAGAAGAA ACTCGCCATG AATAAGGACC AGTGGGACCC GGAGTTCCTG 
AAGGCCATCC CGATCATGAA GACGCGCAGC GGGATGCAGT TTTCCTGTGC GTCTCCCAGC 
CACAGCTGCC CGGAGAACAC GTATGAGGTG TGGGTGTGTA ACAGCGATGG TTACGTTGGT 
CAGGTGTGCC TGCTGAACAT CCGGGACGAG CCCACGGTGG AGGCCTGCAT CGCCGTCTGC 
TCCGCTCGCA TCATCTGCAT CGCCACTGTT CCGGGACTCA AGAACAGAGA GCGTGTGGTT 
CACTCCACAC CGACGGCTCC ATCAGATTCA AACTCTGCAG CGACAACTCA ACCACAGCTC 
CACATCTGCA TCTCTGGCTC GTCTCTGGAG CTCAGCGAGC CGCCAGCGGT GCCCACTGCA 
GAACTCGTGC CCTTCGACAG CGACGACACT GACGATGAGG ACTCGCCCAG CCCTTCATCA 
ACACTGCAAA GCCAGGCCTC ACACTCCACC ATATCATCCA GCTTCGGCAA TGATGAAGCG 
GTGGTGGCGA AGGACATGGC CACAGAGACC ACCAGCTCAG AGGAGGAGCA GGAGTTCCCA 
GTGCCCAGCT CTTTTGGAGC CCCTCGCCTC AGTGCTGAGA GCCCCATGGA TGGACGAGCC 
ATGCGCCGCT CCAGTCGAGG CTCCTTCACC CGCGCCAGCC TGGAGGACCT GCTGAGCATC 
GACCCCGAGG CCTACCAGAG CTCCGTGTGG CTCGGCACCG AGGATGGATG CATCCATGTT 
TACCAGTCAT CAGACAACAT CAGAAACCGG AAGAACAGTA TGAAGATGCA ACACTCGGCT 
TCAGTCCTTT GTATCCTGTA TCTTGACAAT AAAGTGTTTG TGTCTCTGGC AAACGGAGAG 
GTTATTGTGT ATCAGCGGGA GGCAGGGAGT TTCTGGGATC CTCAGAGCTC TCAGACTCTG 
TGTCTCGGGT CTCCAAGCGG ACCTGTGACT AAAATGGTTC CTGTGGCGGG AAAACTGTGG 
TGTGGCTGTC AGAACCGCAT CCTTATCATC AACACCAGCA CTTTAACACA AGAGCACTGG 
TTTCAGGTGG GTCAGGACAG CGGGCGCTGC GTGACCAGTA TGGTGTGTTT TGGTAAAGGT 
GTCTGGATCG CTCTGCAGGG CAGCGCTCAG GTGCGTCTCT ATCACTCCTC CACATACGAG 
AGTCTCACTG AGGTCGACGT CGCTCCAGCC GTTCACAAGA TGCTGGCAGG CTCTGATGCT 
ATTATACGTC AGCACAAGGC GGCGTGTTTG CGGATCACAG CTCTTCTGGC CTGTAAGGAT 
CTGCTGTGGA TCGGCACCAG TGCAGGGGTG GTGTTGACTC TGGTGGTCCC GCCGGTGTCC 
TCCAGCACAG GATCTGGATC TCTGCGTGTC CCGCTGATCC CCATGGGCTC TGCTCACGGA 
CACACTGGAC ATGTGCGCTT TCTGACCTGC ATCGAGCTGC CAGAGGGATT CGACGTGTCT 
TTTCCTCCGC CAGCAGAGTC AGCATCACAG AACAGCTCAG ACAGCGGCAC GGGAGGCCTA 
CAGAGGCGAG ATTCAGCCAG ACGACGCGCC TCCACCCTCC TCCCCACCAA ATCCAACCTG 
CTGGTCATCA GCGGCGGCGA TGGCTATGAA GACTTCAGAC TGACCAATAG CAGCGAGACG 
GTGGGGCGGG ACGACAGCAC CAACCACCTA TTGCTGTGGC GTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-248G20.5 ( XM_003200414.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003200462.1 Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.

Price & Availability↑

CloneID	ODa23018
Clone ID Related Accession (Same CDS sequence)	XM_003200414.5 , XM_003200414.4
Accession Version	XM_003200414.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho guanine nucleotide exchange factor 17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200414.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGA TGGTACGGAC CCTCGCCCAG TTCACCATCA CCCTGGAGGA GATGCAGGAG 
AGCGGAGAGA GCATCAGTGA TGATGGTGGA GGTGTGGGTG AAGAGGTGGA GCGCTCCGTC 
ACTGCCGAGC CCAATGGAAA CGGCATTGTG GGAAACACTG GAGCTCCTCA GACCATTGCT 
GAGGATATGA GAAGACATGT GATGATGAAT CTGCTGGACG CTGAACAGTC TTACGTGGAG 
TCTCTGCGCA CACTCATTCA GGGCTACATG AAGCCGCTGA AGCATCCAGA AAACCCGCCT 
TTGTGTGACC CGTCACTTGT GGACGAGATG TTTTACCAGA TCCCAGAAAT CCTGGAGCAC 
CATGAGCAGT TTCTGGAGCA GGTGCTGGAC TGTGTTCACC AGTGGCACGA CAAGCAGACC 
ATCGGACACG TGCTCATTCA GTCGTTTTCC AAAGAGACTC TGGCTAATAT TTATTCTGCA 
TACATCGACA ATTTCATGAA CGCCAAAGAC GCTGTGAGAA TCGCCAAGGA GGCCAAACCT 
GCTTTCCTCA AGTTTCTGGA GCAAAGCATG CGTGAGAACA GAGAGAAACA AGCTCTGGGT 
GACCTGATGA TAAAGCCGGT TCAGAGAATC CCTCGATACG AGCTGCTGGT CAAGGATCTG 
CTGAAACACA CTCCTGAGGA TCATCCAGAT TACCTGTTTC TGTTGGATGC TCAGAAGAAC 
ATCAAGCGTT TGGCGGAGCG GATCAACAAG GGCAGACGCA GTGCAGAGGA GCTGGAGAGA 
GAAACACGCG TCATGCAGGA GATCGAGGCG CACATCGAGG GTGTAGAACA TATCCTAAAC 
CCACAGAGAA AGTTCCTGAG ACAAGAGATG GTGGTGGAAG CGAAAACGGT GGGCGGGAAG 
AAGGACCGCT CTCTGTTTCT GTTCAGCGAT CTGCTGATCT GCACCACACT CAAGAGGAAA 
TCTGGCTCTC TGCGCAGGAG CTCCATGAGT TTATATTCTG CTGCTAGTGT CATCGACACC 
TCCAGTAAAT ACAAGTTCCT GTGGAAACTG CCTTTAGAAG ACATAGAGGT GGTGAAAGGT 
TCTAATCAAG CCACTAACAG AGAAAATGTC CAGAAAACCA TCAGTCGTCT GGATGAAGAT 
CTCAGCACAC TTGGACAAGT CAGCAAACTG TCAGAAACAC TCAGTTTCCC CCATCAGAGT 
TTGGACGATG TGATTAAGGA TCTGATGGCG TCGGTTCACC GTGAGCTGGC CGAGAAGCAG 
TCTCTGGCCT TCAGCATGAC TTTACTGCCC ACTAAACTGG AGCTGAGCTG CACTTCAGCC 
GAGAGCAGCT TCATCTTTGA GTTCAGCAGT CCGGACACTC GCAGCAACTT CGAGCAGGCC 
TTCGAGGAGG CCAAGAAGAA ACTCGCCATG AATAAGGACC AGTGGGACCC GGAGTTCCTG 
AAGGCCATCC CGATCATGAA GACGCGCAGC GGGATGCAGT TTTCCTGTGC GTCTCCCAGC 
CACAGCTGCC CGGAGAACAC GTATGAGGTG TGGGTGTGTA ACAGCGATGG TTACGTTGGT 
CAGGTGTGCC TGCTGAACAT CCGGGACGAG CCCACGGTGG AGGCCTGCAT CGCCGTCTGC 
TCCGCTCGCA TCATCTGCAT CGCCACTGTT CCGGGACTCA AGAACAGAGA GCGTGTGGTT 
CACTCCACAC CGACGGCTCC ATCAGATTCA AACTCTGCAG CGACAACTCA ACCACAGCTC 
CACATCTGCA TCTCTGGCTC GTCTCTGGAG CTCAGCGAGC CGCCAGCGGT GCCCACTGCA 
GAACTCGTGC CCTTCGACAG CGACGACACT GACGATGAGG ACTCGCCCAG CCCTTCATCA 
ACACTGCAAA GCCAGGCCTC ACACTCCACC ATATCATCCA GCTTCGGCAA TGATGAAGCG 
GTGGTGGCGA AGGACATGGC CACAGAGACC ACCAGCTCAG AGGAGGAGCA GGAGTTCCCA 
GTGCCCAGCT CTTTTGGAGC CCCTCGCCTC AGTGCTGAGA GCCCCATGGA TGGACGAGCC 
ATGCGCCGCT CCAGTCGAGG CTCCTTCACC CGCGCCAGCC TGGAGGACCT GCTGAGCATC 
GACCCCGAGG CCTACCAGAG CTCCGTGTGG CTCGGCACCG AGGATGGATG CATCCATGTT 
TACCAGTCAT CAGACAACAT CAGAAACCGG AAGAACAGTA TGAAGATGCA ACACTCGGCT 
TCAGTCCTTT GTATCCTGTA TCTTGACAAT AAAGTGTTTG TGTCTCTGGC AAACGGAGAG 
GTTATTGTGT ATCAGCGGGA GGCAGGGAGT TTCTGGGATC CTCAGAGCTC TCAGACTCTG 
TGTCTCGGGT CTCCAAGCGG ACCTGTGACT AAAATGGTTC CTGTGGCGGG AAAACTGTGG 
TGTGGCTGTC AGAACCGCAT CCTTATCATC AACACCAGCA CTTTAACACA AGAGCACTGG 
TTTCAGGTGG GTCAGGACAG CGGGCGCTGC GTGACCAGTA TGGTGTGTTT TGGTAAAGGT 
GTCTGGATCG CTCTGCAGGG CAGCGCTCAG GTGCGTCTCT ATCACTCCTC CACATACGAG 
AGTCTCACTG AGGTCGACGT CGCTCCAGCC GTTCACAAGA TGCTGGCAGG CTCTGATGCT 
ATTATACGTC AGCACAAGGC GGCGTGTTTG CGGATCACAG CTCTTCTGGC CTGTAAGGAT 
CTGCTGTGGA TCGGCACCAG TGCAGGGGTG GTGTTGACTC TGGTGGTCCC GCCGGTGTCC 
TCCAGCACAG GATCTGGATC TCTGCGTGTC CCGCTGATCC CCATGGGCTC TGCTCACGGA 
CACACTGGAC ATGTGCGCTT TCTGACCTGC ATCGAGCTGC CAGAGGGATT CGACGTGTCT 
TTTCCTCCGC CAGCAGAGTC AGCATCACAG AACAGCTCAG ACAGCGGCAC GGGAGGCCTA 
CAGAGGCGAG ATTCAGCCAG ACGACGCGCC TCCACCCTCC TCCCCACCAA ATCCAACCTG 
CTGGTCATCA GCGGCGGCGA TGGCTATGAA GACTTCAGAC TGACCAATAG CAGCGAGACG 
GTGGGGCGGG ACGACAGCAC CAACCACCTA TTGCTGTGGC GTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200462.1
CDS	411..3518
Translation	MLQMVRTLAQFTITLEEMQESGESISDDGGGVGEEVERSVTAEPNGNGIVGNTGAPQTIAEDMRRHVMMNLLDAEQSYVESLRTLIQGYMKPLKHPENPPLCDPSLVDEMFYQIPEILEHHEQFLEQVLDCVHQWHDKQTIGHVLIQSFSKETLANIYSAYIDNFMNAKDAVRIAKEAKPAFLKFLEQSMRENREKQALGDLMIKPVQRIPRYELLVKDLLKHTPEDHPDYLFLLDAQKNIKRLAERINKGRRSAEELERETRVMQEIEAHIEGVEHILNPQRKFLRQEMVVEAKTVGGKKDRSLFLFSDLLICTTLKRKSGSLRRSSMSLYSAASVIDTSSKYKFLWKLPLEDIEVVKGSNQATNRENVQKTISRLDEDLSTLGQVSKLSETLSFPHQSLDDVIKDLMASVHRELAEKQSLAFSMTLLPTKLELSCTSAESSFIFEFSSPDTRSNFEQAFEEAKKKLAMNKDQWDPEFLKAIPIMKTRSGMQFSCASPSHSCPENTYEVWVCNSDGYVGQVCLLNIRDEPTVEACIAVCSARIICIATVPGLKNRERVVHSTPTAPSDSNSAATTQPQLHICISGSSLELSEPPAVPTAELVPFDSDDTDDEDSPSPSSTLQSQASHSTISSSFGNDEAVVAKDMATETTSSEEEQEFPVPSSFGAPRLSAESPMDGRAMRRSSRGSFTRASLEDLLSIDPEAYQSSVWLGTEDGCIHVYQSSDNIRNRKNSMKMQHSASVLCILYLDNKVFVSLANGEVIVYQREAGSFWDPQSSQTLCLGSPSGPVTKMVPVAGKLWCGCQNRILIINTSTLTQEHWFQVGQDSGRCVTSMVCFGKGVWIALQGSAQVRLYHSSTYESLTEVDVAPAVHKMLAGSDAIIRQHKAACLRITALLACKDLLWIGTSAGVVLTLVVPPVSSSTGSGSLRVPLIPMGSAHGHTGHVRFLTCIELPEGFDVSFPPPAESASQNSSDSGTGGLQRRDSARRRASTLLPTKSNLLVISGGDGYEDFRLTNSSETVGRDDSTNHLLLWRV

Target ORF information:

RefSeq Version	XM_003200414.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-248g20.5 (si:ch211-248g20.5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200414.4

ORF Insert Sequence:

ATGCTGCAGA TGGTACGGAC CCTCGCCCAG TTCACCATCA CCCTGGAGGA GATGCAGGAG 
AGCGGAGAGA GCATCAGTGA TGATGGTGGA GGTGTGGGTG AAGAGGTGGA GCGCTCCGTC 
ACTGCCGAGC CCAATGGAAA CGGCATTGTG GGAAACACTG GAGCTCCTCA GACCATTGCT 
GAGGATATGA GAAGACATGT GATGATGAAT CTGCTGGACG CTGAACAGTC TTACGTGGAG 
TCTCTGCGCA CACTCATTCA GGGCTACATG AAGCCGCTGA AGCATCCAGA AAACCCGCCT 
TTGTGTGACC CGTCACTTGT GGACGAGATG TTTTACCAGA TCCCAGAAAT CCTGGAGCAC 
CATGAGCAGT TTCTGGAGCA GGTGCTGGAC TGTGTTCACC AGTGGCACGA CAAGCAGACC 
ATCGGACACG TGCTCATTCA GTCGTTTTCC AAAGAGACTC TGGCTAATAT TTATTCTGCA 
TACATCGACA ATTTCATGAA CGCCAAAGAC GCTGTGAGAA TCGCCAAGGA GGCCAAACCT 
GCTTTCCTCA AGTTTCTGGA GCAAAGCATG CGTGAGAACA GAGAGAAACA AGCTCTGGGT 
GACCTGATGA TAAAGCCGGT TCAGAGAATC CCTCGATACG AGCTGCTGGT CAAGGATCTG 
CTGAAACACA CTCCTGAGGA TCATCCAGAT TACCTGTTTC TGTTGGATGC TCAGAAGAAC 
ATCAAGCGTT TGGCGGAGCG GATCAACAAG GGCAGACGCA GTGCAGAGGA GCTGGAGAGA 
GAAACACGCG TCATGCAGGA GATCGAGGCG CACATCGAGG GTGTAGAACA TATCCTAAAC 
CCACAGAGAA AGTTCCTGAG ACAAGAGATG GTGGTGGAAG CGAAAACGGT GGGCGGGAAG 
AAGGACCGCT CTCTGTTTCT GTTCAGCGAT CTGCTGATCT GCACCACACT CAAGAGGAAA 
TCTGGCTCTC TGCGCAGGAG CTCCATGAGT TTATATTCTG CTGCTAGTGT CATCGACACC 
TCCAGTAAAT ACAAGTTCCT GTGGAAACTG CCTTTAGAAG ACATAGAGGT GGTGAAAGGT 
TCTAATCAAG CCACTAACAG AGAAAATGTC CAGAAAACCA TCAGTCGTCT GGATGAAGAT 
CTCAGCACAC TTGGACAAGT CAGCAAACTG TCAGAAACAC TCAGTTTCCC CCATCAGAGT 
TTGGACGATG TGATTAAGGA TCTGATGGCG TCGGTTCACC GTGAGCTGGC CGAGAAGCAG 
TCTCTGGCCT TCAGCATGAC TTTACTGCCC ACTAAACTGG AGCTGAGCTG CACTTCAGCC 
GAGAGCAGCT TCATCTTTGA GTTCAGCAGT CCGGACACTC GCAGCAACTT CGAGCAGGCC 
TTCGAGGAGG CCAAGAAGAA ACTCGCCATG AATAAGGACC AGTGGGACCC GGAGTTCCTG 
AAGGCCATCC CGATCATGAA GACGCGCAGC GGGATGCAGT TTTCCTGTGC GTCTCCCAGC 
CACAGCTGCC CGGAGAACAC GTATGAGGTG TGGGTGTGTA ACAGCGATGG TTACGTTGGT 
CAGGTGTGCC TGCTGAACAT CCGGGACGAG CCCACGGTGG AGGCCTGCAT CGCCGTCTGC 
TCCGCTCGCA TCATCTGCAT CGCCACTGTT CCGGGACTCA AGAACAGAGA GCGTGTGGTT 
CACTCCACAC CGACGGCTCC ATCAGATTCA AACTCTGCAG CGACAACTCA ACCACAGCTC 
CACATCTGCA TCTCTGGCTC GTCTCTGGAG CTCAGCGAGC CGCCAGCGGT GCCCACTGCA 
GAACTCGTGC CCTTCGACAG CGACGACACT GACGATGAGG ACTCGCCCAG CCCTTCATCA 
ACACTGCAAA GCCAGGCCTC ACACTCCACC ATATCATCCA GCTTCGGCAA TGATGAAGCG 
GTGGTGGCGA AGGACATGGC CACAGAGACC ACCAGCTCAG AGGAGGAGCA GGAGTTCCCA 
GTGCCCAGCT CTTTTGGAGC CCCTCGCCTC AGTGCTGAGA GCCCCATGGA TGGACGAGCC 
ATGCGCCGCT CCAGTCGAGG CTCCTTCACC CGCGCCAGCC TGGAGGACCT GCTGAGCATC 
GACCCCGAGG CCTACCAGAG CTCCGTGTGG CTCGGCACCG AGGATGGATG CATCCATGTT 
TACCAGTCAT CAGACAACAT CAGAAACCGG AAGAACAGTA TGAAGATGCA ACACTCGGCT 
TCAGTCCTTT GTATCCTGTA TCTTGACAAT AAAGTGTTTG TGTCTCTGGC AAACGGAGAG 
GTTATTGTGT ATCAGCGGGA GGCAGGGAGT TTCTGGGATC CTCAGAGCTC TCAGACTCTG 
TGTCTCGGGT CTCCAAGCGG ACCTGTGACT AAAATGGTTC CTGTGGCGGG AAAACTGTGG 
TGTGGCTGTC AGAACCGCAT CCTTATCATC AACACCAGCA CTTTAACACA AGAGCACTGG 
TTTCAGGTGG GTCAGGACAG CGGGCGCTGC GTGACCAGTA TGGTGTGTTT TGGTAAAGGT 
GTCTGGATCG CTCTGCAGGG CAGCGCTCAG GTGCGTCTCT ATCACTCCTC CACATACGAG 
AGTCTCACTG AGGTCGACGT CGCTCCAGCC GTTCACAAGA TGCTGGCAGG CTCTGATGCT 
ATTATACGTC AGCACAAGGC GGCGTGTTTG CGGATCACAG CTCTTCTGGC CTGTAAGGAT 
CTGCTGTGGA TCGGCACCAG TGCAGGGGTG GTGTTGACTC TGGTGGTCCC GCCGGTGTCC 
TCCAGCACAG GATCTGGATC TCTGCGTGTC CCGCTGATCC CCATGGGCTC TGCTCACGGA 
CACACTGGAC ATGTGCGCTT TCTGACCTGC ATCGAGCTGC CAGAGGGATT CGACGTGTCT 
TTTCCTCCGC CAGCAGAGTC AGCATCACAG AACAGCTCAG ACAGCGGCAC GGGAGGCCTA 
CAGAGGCGAG ATTCAGCCAG ACGACGCGCC TCCACCCTCC TCCCCACCAA ATCCAACCTG 
CTGGTCATCA GCGGCGGCGA TGGCTATGAA GACTTCAGAC TGACCAATAG CAGCGAGACG 
GTGGGGCGGG ACGACAGCAC CAACCACCTA TTGCTGTGGC GTGTCTAA