bmp6 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp6
Entrez Gene ID	103376944
Full Name	bone morphogenetic protein 6
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328247.1	NP_001315176.1	bone morphogenetic protein 6 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cloning, expression profiling and promoter functional analysis of Bone morphogenetic protein 6 and 7 in tongue sole (Cynoglossus semilaevis).
Ma Q, Feng W, Zhuang Z, Liu S
Fish physiology and biochemistry43(2)435-454(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

As a first approach to elucidating the role of BMPs in regulating fish bone formation and growth, we describe the cloning, expression profiling and promoter functional analysis of bmp6 and bmp7 in tongue sole (Cynoglossus semilaevis). The results revealed that bmp6 was predominantly expressed in the gonad, and bmp7 exhibited the highest expression level in the dorsal fin [BMP7]
Title: Cloning, expression profiling and promoter functional analysis of Bone morphogenetic protein 6 and 7 in tongue sole (Cynoglossus semilaevis).

The following bmp6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy63114	NM_001328247.1 Latest version!	Cynoglossus semilaevis bone morphogenetic protein 6 (bmp6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp6 ( NM_001328247.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> NP_001315176.1 Cynoglossus semilaevis bone morphogenetic protein 6 (bmp6), mRNA.

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CloneID	OCy63114
Clone ID Related Accession (Same CDS sequence)	NM_001328247.1
Accession Version	NM_001328247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Cynoglossus semilaevis(tongue sole)
Product	bone morphogenetic protein 6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KC422340.1. On Jun 9, 2016 this sequence version replaced XM_008307517.2. ##Evidence-Data-START## Transcript exon combination :: KC422340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN08193245 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAACT GCTGTCTTGC TTTAGTCGGA TTGTGGTGGA GCGCTTATTA CTGCATCGTC 
CTGGTGTCGG GGAGTAATTA TTCACTGGAT GGGAACAACG AAGTGCACCA GAGTTTCATC 
CACCGGCGGC TGCGGACGCA CGAGAAGAGG GAGATGCAGA AGGAAATTCT CTCCATCCTG 
GGCCTGCCGC ACAGACCCAG ACCGCACATA TCGCACGGAA AGTTCAACTC CGCTCCACTT 
TTCATGCTGG ACTTGTACAA CACCATATCC ACGGACGAGA AAAGCCATGT GGAAGGCTTG 
CTGGACCGAT ATCAGTCGGC GCGCACAACT CAGAGCCCTC ATCTGGCCAC GTATCAAGAA 
ACTGCCTTTC TGAATGATGC AGATATGGTG ATGAGCTTCG TCAATCTGGT GGAGTACGAC 
AGCGAGTTCT CCCCACAGCG CCGGCACCAC AAGGAGTTTA AGTTCAACCT CTCGCAGATC 
CCGGAGGGCG AGGCCGTCAC TGCAGCCGAG TTCCGTCTCT ACAAGGAGTG TGTGAGTCGC 
ACATTCCGCA ATGACACCTT CATACTTAAA GTATACCAGG TGGTCAAGGA GCACCCAGAC 
AGGGAGGCAG ACTTGTTCCT GCTGGAATCT CGCCACTTAT GGGCAGCAGA AGAAGGCTGG 
CTGGAGTTCG ACATCACAGC CACCAGTAAC CTGTGGGTGA TGAGTCCAGA GCACAACCTG 
GGGCTGCAGA TCAGTCTGGA GACCAGCAGT GGACAGAGCA TCACACCCAA GGAGGCGGGG 
CTCGTGGGAC GTGATGGCGC GCTGGAGAAA CAACCTTTCA TGGTGGCATT CTTCAAAGTC 
AGTGAAGTGT ACGTCCGCAC CACCCGCTCC ACTGGAGGCA AAAGGCGCCA GCAGAATCGC 
AACAGGTCCA CGCAACCACA GGAGGGATCC AGAACGTTGG GCCTTGCAGA TTATAACAGC 
AGTGATCAGA AAACAGCCTG CAGGAGGCAT GAGCTCTACG TCAGCTTCCG AGAGCTGGGC 
TGGCAGGACT GGATCATTGC TCCTGAGGGC TACGCTGCAA ACTACTGTGA TGGAGAGTGT 
TCCTTTCCCC TCAACGCCCA CATGAATGCC ACCAATCACG CTATTGTACA GACACTGGTG 
CACCCAATGA ATCCTGAGAA CGTACCGAAG CCGTGCTGCG CCCCCACCAA ACTCCACGCC 
ATCTCGGTGC TTTACTTTGA CGACAACTCC AATGTCATTC TGAAAAAATA CAAGAACATG 
GTAGTGCGGG CCTGCGGCTG CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315176.1
CDS	472..1758
Translation	MSNCCLALVGLWWSAYYCIVLVSGSNYSLDGNNEVHQSFIHRRLRTHEKREMQKEILSILGLPHRPRPHISHGKFNSAPLFMLDLYNTISTDEKSHVEGLLDRYQSARTTQSPHLATYQETAFLNDADMVMSFVNLVEYDSEFSPQRRHHKEFKFNLSQIPEGEAVTAAEFRLYKECVSRTFRNDTFILKVYQVVKEHPDREADLFLLESRHLWAAEEGWLEFDITATSNLWVMSPEHNLGLQISLETSSGQSITPKEAGLVGRDGALEKQPFMVAFFKVSEVYVRTTRSTGGKRRQQNRNRSTQPQEGSRTLGLADYNSSDQKTACRRHELYVSFRELGWQDWIIAPEGYAANYCDGECSFPLNAHMNATNHAIVQTLVHPMNPENVPKPCCAPTKLHAISVLYFDDNSNVILKKYKNMVVRACGCH

Target ORF information:

RefSeq Version	NM_001328247.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis bone morphogenetic protein 6 (bmp6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328247.1

ORF Insert Sequence:

ATGTCCAACT GCTGTCTTGC TTTAGTCGGA TTGTGGTGGA GCGCTTATTA CTGCATCGTC 
CTGGTGTCGG GGAGTAATTA TTCACTGGAT GGGAACAACG AAGTGCACCA GAGTTTCATC 
CACCGGCGGC TGCGGACGCA CGAGAAGAGG GAGATGCAGA AGGAAATTCT CTCCATCCTG 
GGCCTGCCGC ACAGACCCAG ACCGCACATA TCGCACGGAA AGTTCAACTC CGCTCCACTT 
TTCATGCTGG ACTTGTACAA CACCATATCC ACGGACGAGA AAAGCCATGT GGAAGGCTTG 
CTGGACCGAT ATCAGTCGGC GCGCACAACT CAGAGCCCTC ATCTGGCCAC GTATCAAGAA 
ACTGCCTTTC TGAATGATGC AGATATGGTG ATGAGCTTCG TCAATCTGGT GGAGTACGAC 
AGCGAGTTCT CCCCACAGCG CCGGCACCAC AAGGAGTTTA AGTTCAACCT CTCGCAGATC 
CCGGAGGGCG AGGCCGTCAC TGCAGCCGAG TTCCGTCTCT ACAAGGAGTG TGTGAGTCGC 
ACATTCCGCA ATGACACCTT CATACTTAAA GTATACCAGG TGGTCAAGGA GCACCCAGAC 
AGGGAGGCAG ACTTGTTCCT GCTGGAATCT CGCCACTTAT GGGCAGCAGA AGAAGGCTGG 
CTGGAGTTCG ACATCACAGC CACCAGTAAC CTGTGGGTGA TGAGTCCAGA GCACAACCTG 
GGGCTGCAGA TCAGTCTGGA GACCAGCAGT GGACAGAGCA TCACACCCAA GGAGGCGGGG 
CTCGTGGGAC GTGATGGCGC GCTGGAGAAA CAACCTTTCA TGGTGGCATT CTTCAAAGTC 
AGTGAAGTGT ACGTCCGCAC CACCCGCTCC ACTGGAGGCA AAAGGCGCCA GCAGAATCGC 
AACAGGTCCA CGCAACCACA GGAGGGATCC AGAACGTTGG GCCTTGCAGA TTATAACAGC 
AGTGATCAGA AAACAGCCTG CAGGAGGCAT GAGCTCTACG TCAGCTTCCG AGAGCTGGGC 
TGGCAGGACT GGATCATTGC TCCTGAGGGC TACGCTGCAA ACTACTGTGA TGGAGAGTGT 
TCCTTTCCCC TCAACGCCCA CATGAATGCC ACCAATCACG CTATTGTACA GACACTGGTG 
CACCCAATGA ATCCTGAGAA CGTACCGAAG CCGTGCTGCG CCCCCACCAA ACTCCACGCC 
ATCTCGGTGC TTTACTTTGA CGACAACTCC AATGTCATTC TGAAAAAATA CAAGAACATG 
GTAGTGCGGG CCTGCGGCTG CCACTGA