LOC101218826 gene cDNA ORF clone, Cucumis sativus(cucumber)

mRNA	Protein	Name
XM_011652507.2	XP_011650809.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_004134305.2	XP_004134353.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_011652509.2	XP_011650811.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X2
XM_011652508.1	XP_011650810.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_004134305.3	XP_004134353.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_031881971.1	XP_031737831.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_011652507.1	XP_011650809.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
XM_011652509.1	XP_011650811.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X2
XM_011652508.2	XP_011650810.1	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu59283	XM_011652509.2 Latest version!	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCu59282	XM_004134305.3 Latest version!	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCu59282	XM_011652508.2 Latest version!	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCu59282	XM_011652507.2 Latest version!	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCu59282	XM_031881971.1 Latest version!	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCu59282	XM_004134305.2	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCu59282	XM_011652507.1	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCu59283	XM_011652509.1	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCu59282	XM_011652508.1	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

LOC101218826 ( XM_011652509.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650811.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCu59283
Clone ID Related Accession (Same CDS sequence)	XM_011652509.2 , XM_011652509.1
Accession Version	XM_011652509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011652509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTACT GGTATTTCGC ACCCTTTGCA AGTGCTGTGT TACAGATGGG AATGAAGGAG 
GATACTGATG AAGTCACAAC CAAGACTCGC ATTTTATCTC TTGAGCTTCT GCAGGGTTTA 
TTAGAAGGGG TTAGCCAGAC CTTTACAAAA GACTTCCATT TTATTGACTC AGTGAAAGCA 
TATCTATCAT ATGCTTTACT GCGAGCTTCT GTCTCACAAC CTCCTGTAAT ATTTCAGTAC 
GCAACTGGAA TATTCTCTGT GCTATTGTTG CGATTCAGGG AAAGTCTTAA AGGTGAAATT 
GGCATATTCT TTCCCTTGAT AGTTCTGAGA TCATTGGATG GCACAGATTT TCCTGTTAAT 
CAAAAAACAA GTGTTTTGAA GATGCTTGAG AAAATATGTA GGGAACCTCA AATCCTTGTT 
GACATATTTG TCAACTATGA CTGTGATCTG GAGGCTCCAA ATTTGTTTGA ACGCATGGTA 
ACCACTTTAT CTAAACTTTC TCAAGGCACC CAAAATGCAG ATCCAAATTT GGCAGCTTTA 
TCCCAAGCAA CTTCAATTAA AGGATCGTCA CTTCAATGCC TTGTGAATGT GCTTAAATCA 
TTAGTTGACT GGGAGAAGTC ACGTCTTCAT TCAGAAAAGG AGGGCTTGGT TCATTCGTCT 
GAAGAAGAGT CTTCAGGCAA TGAAAATCTT GAGGTAAAGA GCAGGGAAGA TGTGACAGGT 
AATTTTGAGA AGGCAAAAGC TCACAAGTCC ACGGTGGAAG CTGCCATCTC GGAGTTCAAT 
AGGAAACCAG TAAAGGGTGT CGAGTATCTG ATTTCAAATA AGTTGGTGGA GAATACACCA 
TCTTCAGTTG CCCTCTTTCT CCGAAATACT CCCAGTTTGG ACAAGACTAT GATTGGCGAT 
TATCTTGGTC AGCATGAAGA GTTTCCTGTT GCAGTCATGC ATGCATATGT AGATTCGATG 
AAATTTTCCG GCATGAAGTT TGATGCTGCA ATTCGTGAAT TTCTCAAAGG TTTCCGTCTT 
CCAGGGGAAG CTCAAAAAAT TGATCGCATC ATGGAAAAAT TTGCAGAACG ATATTGTGCA 
GACAACCCAG GCCTTTTCAA GAATGCAGAC ACTGCATATG TTCTAGCTTA TGCAGTGATT 
ATGTTGAACA CCGATGCCCA TAACCCTATG GTGTGGCCTA AGATGTCGAA GTCCGATTTC 
ACTCGCATGA ATGTCATGAA CGATCCAGAA GATTGTGCCC CTACAGAACT CCTGGAAGAG 
ATATATGATT CTATAGTTAA AGAAGAAATC AAAATGAAAG ATGACCTTCT TGACAAAGCA 
AAAAGCCGCA GGCTGGAGGT TGAAGAGAAG GGAGGCCTTG TGAGTATTCT TAACTTGGCT 
CTTCCAAGAA GAAAGTCCTC TACTGAAGCC CAATCCGAGA GTGAGGCTAT TATTAAGCAA 
ACACAAGTTA TATTCCGAAA TCAAGGAGCA AAGAGAGGCG TTTTTTATAC CTCACAGAGA 
ATTGAACTTG TAAGGCCTAT GGTTGAGGCT GTAGGATGGC CTTTACTTGC TACTTTCTCC 
GTTACCATGG AGGAAGGTGA TAATAAACCT AGGGTTGTTC TTTGTATGGA GGGGTTTAGA 
GCTGGTATAC ATATTACACA TGTTCTTGGA ATGGATACCA TGCGCTATGC ATTTCTAACA 
TCTCTCGTCA GGTTTACTTT CTTGCATGCG CCAAAGGAAA TGCGTAGTAA AAATGTAGAA 
GCATTACGCA CGCTATTGGC TCTGTGCGAT TTAGAGACAG AATCCCTTCA AGACACTTGG 
AATGCAGTTT TAGAATGTGT GTCCCGTCTC GAATTCATCA CATCAACACC TTCAATTGCA 
GCCACTGTCA TGTATGGATC AAATCAGATT TCCAGAGATG CTGTTGTTCA ATCCCTTAGA 
GAGTTGGCTG GGAAGCCTGC AGATCAAGTA TTTGTGAACA GTGTCAAACT TCCCAGTGAC 
TCTGTTGTGG AGTTCTTCAC TGCTCTATGT GGTGTCTCTG CTGAAGAACT GAAACAAACA 
CCTGCACGTG TTTTCAGCTT GCAAAAGCTT GTTGAGATAA GCTATTATAA TATGGCTCGT 
ATACGAATGG TTTGGGCTAG GATATGGTCT GTATTGTCAA ATCACTTTAT TTCTGCTGGG 
AGCCATCATG ATGAGAAGAT TGCCATGTAT GCTATAGATT CTCTGAGGCA GCTTGGGATG 
AAGTACTTGG AGAGAGCTGA ACTTGCCAAC TTCACATTCC AAAATGACAT TCTTAAGCCA 
TTTGTTGTTC TCATGCGTAA CAGTCAAAGT GAATCTATAA GGAGCCTCAT TGTTGACTGC 
ATAGTTCAAA TGATAAAATC AAAAGTTGGT AACATAAAGT CCGGATGGCG AAGTGTTTTC 
ATGATTTTCA CTGCTTCCGC AGATGATGAA TCTGAGTCAA TTGTTGAAAG TGCCTTTGAA 
AATGTTGAAC AGGTTATATT GGAGCACTTT GATCAGGTGG TTGGGGACTG TTTTATGGAC 
TGTGTCAACT GTCTTATAAG GTTCGCCAAT AACAAAAGTT CCCATCGTAT AAGCTTGAAA 
GCTATTGCAC TCCTACGTAT TTGTGAGGAT CGATTGGCAG AGGGCCTCAT ACCCGGTGGT 
GCTTTGAAGC CGATCCACGA CAACGAGAGT GCTGAGCCAG CATTTGATAT GACTGAGCAC 
TATTGGTTCC CAATGCTTGC TGGGTTGTCA GATTTGACAT CAGACCCAAG ACCAGAGGTC 
AGAAGTTGTG CATTGGAGGT TTTGTTTGAC TTGCTGAATG AGAGAGGTTC CAAGTTCTCG 
ATGTCATTTT GGGAGAGCAT TTTTCATCGT GTCTTGTTTC CAATATTTGA TCATTTGAGA 
CATGCTGGGA AGGAAAGTGT GAATTCTAGT GGTGATGAGT GGCTTCGTGA AACAAGTATA 
CACTCGCTTC AGTTGCTTTG CAATCTCTTC AATACATTTT ACAAGGAGGT GTGCTTTATG 
CTACCTCCAC TGTTGAGCTT GCTATTAGAT TGTGCAAAGC AGCCGGAACA ATCTGTGGTC 
TCATTGGCTT TGGGTGCTTT GGTGCATCTA ATTGAAGTGG GTGGACACCA GTTCAGCGAG 
GATGACTGGG ACACATTGTT AAAAAGTATT AGAGATGCTT CATACACAAC TCAACCACTT 
GAATTACTTA ATGCTTTGGG TTTTGAGAAC CCAAGCCATG ATGAGCTGAA TATTGTTGAT 
GATGGTTCCT TGAAGTGGTC TAGCCAGCAA GAAGCAAAGA ACCACCATAT TGATGTAAAT 
GAGCATGGAA AGGTATCTCC TGTTCCCTCA CCAAGGGTTG CAGAAATCAT TACTCGGAGT 
CCCATTGCAG AGTCTGGGTT ACAGATTACC ACAGATGAGT CTGCTGAAGG TATTCCATCG 
CCGTCAACAA GAGCTACAAG AGCTGCTGAA GCTGCAAATC TACAGCGGAG CCAAACAATA 
GGCCAACGAA TTATGGGAAA TATGATGGAC AATATCTTTG TTAGAAGTCT AACTTCAAAA 
TCTAAGGGAC GTGCATCAGA TGCATCTGTG CCATCTTCTC CAATAAGGCT TCCTCCTGAC 
ACTGTGGATC CTGAAGTGAA AGATGATGAG GAAAGTCCAC TATTGGGGAT TGTTCGAGGC 
AAATGCATTA CACAACTACT ACTTCTTGGT GTCATTGATG GAATTCAGAA GAAATACTGG 
GTCAAGCTGA GTGCACCACA AAAGATTGCC ATAATGGACA TTTTGTTATC TTTGCTGGAG 
TTCTCTGCTA CATATAACTC ATACAACAAT CTCAGACAGC GCATGAATCA TATTCCTGAT 
GAGAGGCCTC CACTGAATCT TCTTCGTCAG GAGTTAGCGG GAACTTCAAT TTATCTAGAC 
ATCTTACTTA AAGCAACTTC AGGATTTAAT ACGATTGAAG CAGAACAAGA AAAAATTGCT 
GATAGTCTGG AAGTTGATTC TGAATCACCC AAAGATGATT TAACTTCCAT CCAGGATTCT 
AGTGCAGTAA GCAATGTTGA TGGAATAGCT GAAAACAGGC TAGTGTCATT CTGTGAACAA 
GCTCTTAGGG AGGTATCTGA CCTGCAATCG TCTGCAGTGG AAACCACGCA CATGGATGTT 
CATCGAGTTT TGGAGTTACG TTCTCCAGTG ATTGTTAAGG TGATCAAAGG CATGTGCTTC 
ATGAACAGTC AGATATTCAG AAGACATCTG AGAGAGTTTT ATCCTTTGCT TACAAAACTT 
GTATGCTGTG ACCAGATTGA TATTCGTGGA GCACTTGGTG ATCTCTTCAA GATACAATTG 
AAAGCACTAT TGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650811.1
CDS	181..4518
Translation	MLYWYFAPFASAVLQMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652509.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652509.2

ORF Insert Sequence:

ATGCTCTACT GGTATTTCGC ACCCTTTGCA AGTGCTGTGT TACAGATGGG AATGAAGGAG 
GATACTGATG AAGTCACAAC CAAGACTCGC ATTTTATCTC TTGAGCTTCT GCAGGGTTTA 
TTAGAAGGGG TTAGCCAGAC CTTTACAAAA GACTTCCATT TTATTGACTC AGTGAAAGCA 
TATCTATCAT ATGCTTTACT GCGAGCTTCT GTCTCACAAC CTCCTGTAAT ATTTCAGTAC 
GCAACTGGAA TATTCTCTGT GCTATTGTTG CGATTCAGGG AAAGTCTTAA AGGTGAAATT 
GGCATATTCT TTCCCTTGAT AGTTCTGAGA TCATTGGATG GCACAGATTT TCCTGTTAAT 
CAAAAAACAA GTGTTTTGAA GATGCTTGAG AAAATATGTA GGGAACCTCA AATCCTTGTT 
GACATATTTG TCAACTATGA CTGTGATCTG GAGGCTCCAA ATTTGTTTGA ACGCATGGTA 
ACCACTTTAT CTAAACTTTC TCAAGGCACC CAAAATGCAG ATCCAAATTT GGCAGCTTTA 
TCCCAAGCAA CTTCAATTAA AGGATCGTCA CTTCAATGCC TTGTGAATGT GCTTAAATCA 
TTAGTTGACT GGGAGAAGTC ACGTCTTCAT TCAGAAAAGG AGGGCTTGGT TCATTCGTCT 
GAAGAAGAGT CTTCAGGCAA TGAAAATCTT GAGGTAAAGA GCAGGGAAGA TGTGACAGGT 
AATTTTGAGA AGGCAAAAGC TCACAAGTCC ACGGTGGAAG CTGCCATCTC GGAGTTCAAT 
AGGAAACCAG TAAAGGGTGT CGAGTATCTG ATTTCAAATA AGTTGGTGGA GAATACACCA 
TCTTCAGTTG CCCTCTTTCT CCGAAATACT CCCAGTTTGG ACAAGACTAT GATTGGCGAT 
TATCTTGGTC AGCATGAAGA GTTTCCTGTT GCAGTCATGC ATGCATATGT AGATTCGATG 
AAATTTTCCG GCATGAAGTT TGATGCTGCA ATTCGTGAAT TTCTCAAAGG TTTCCGTCTT 
CCAGGGGAAG CTCAAAAAAT TGATCGCATC ATGGAAAAAT TTGCAGAACG ATATTGTGCA 
GACAACCCAG GCCTTTTCAA GAATGCAGAC ACTGCATATG TTCTAGCTTA TGCAGTGATT 
ATGTTGAACA CCGATGCCCA TAACCCTATG GTGTGGCCTA AGATGTCGAA GTCCGATTTC 
ACTCGCATGA ATGTCATGAA CGATCCAGAA GATTGTGCCC CTACAGAACT CCTGGAAGAG 
ATATATGATT CTATAGTTAA AGAAGAAATC AAAATGAAAG ATGACCTTCT TGACAAAGCA 
AAAAGCCGCA GGCTGGAGGT TGAAGAGAAG GGAGGCCTTG TGAGTATTCT TAACTTGGCT 
CTTCCAAGAA GAAAGTCCTC TACTGAAGCC CAATCCGAGA GTGAGGCTAT TATTAAGCAA 
ACACAAGTTA TATTCCGAAA TCAAGGAGCA AAGAGAGGCG TTTTTTATAC CTCACAGAGA 
ATTGAACTTG TAAGGCCTAT GGTTGAGGCT GTAGGATGGC CTTTACTTGC TACTTTCTCC 
GTTACCATGG AGGAAGGTGA TAATAAACCT AGGGTTGTTC TTTGTATGGA GGGGTTTAGA 
GCTGGTATAC ATATTACACA TGTTCTTGGA ATGGATACCA TGCGCTATGC ATTTCTAACA 
TCTCTCGTCA GGTTTACTTT CTTGCATGCG CCAAAGGAAA TGCGTAGTAA AAATGTAGAA 
GCATTACGCA CGCTATTGGC TCTGTGCGAT TTAGAGACAG AATCCCTTCA AGACACTTGG 
AATGCAGTTT TAGAATGTGT GTCCCGTCTC GAATTCATCA CATCAACACC TTCAATTGCA 
GCCACTGTCA TGTATGGATC AAATCAGATT TCCAGAGATG CTGTTGTTCA ATCCCTTAGA 
GAGTTGGCTG GGAAGCCTGC AGATCAAGTA TTTGTGAACA GTGTCAAACT TCCCAGTGAC 
TCTGTTGTGG AGTTCTTCAC TGCTCTATGT GGTGTCTCTG CTGAAGAACT GAAACAAACA 
CCTGCACGTG TTTTCAGCTT GCAAAAGCTT GTTGAGATAA GCTATTATAA TATGGCTCGT 
ATACGAATGG TTTGGGCTAG GATATGGTCT GTATTGTCAA ATCACTTTAT TTCTGCTGGG 
AGCCATCATG ATGAGAAGAT TGCCATGTAT GCTATAGATT CTCTGAGGCA GCTTGGGATG 
AAGTACTTGG AGAGAGCTGA ACTTGCCAAC TTCACATTCC AAAATGACAT TCTTAAGCCA 
TTTGTTGTTC TCATGCGTAA CAGTCAAAGT GAATCTATAA GGAGCCTCAT TGTTGACTGC 
ATAGTTCAAA TGATAAAATC AAAAGTTGGT AACATAAAGT CCGGATGGCG AAGTGTTTTC 
ATGATTTTCA CTGCTTCCGC AGATGATGAA TCTGAGTCAA TTGTTGAAAG TGCCTTTGAA 
AATGTTGAAC AGGTTATATT GGAGCACTTT GATCAGGTGG TTGGGGACTG TTTTATGGAC 
TGTGTCAACT GTCTTATAAG GTTCGCCAAT AACAAAAGTT CCCATCGTAT AAGCTTGAAA 
GCTATTGCAC TCCTACGTAT TTGTGAGGAT CGATTGGCAG AGGGCCTCAT ACCCGGTGGT 
GCTTTGAAGC CGATCCACGA CAACGAGAGT GCTGAGCCAG CATTTGATAT GACTGAGCAC 
TATTGGTTCC CAATGCTTGC TGGGTTGTCA GATTTGACAT CAGACCCAAG ACCAGAGGTC 
AGAAGTTGTG CATTGGAGGT TTTGTTTGAC TTGCTGAATG AGAGAGGTTC CAAGTTCTCG 
ATGTCATTTT GGGAGAGCAT TTTTCATCGT GTCTTGTTTC CAATATTTGA TCATTTGAGA 
CATGCTGGGA AGGAAAGTGT GAATTCTAGT GGTGATGAGT GGCTTCGTGA AACAAGTATA 
CACTCGCTTC AGTTGCTTTG CAATCTCTTC AATACATTTT ACAAGGAGGT GTGCTTTATG 
CTACCTCCAC TGTTGAGCTT GCTATTAGAT TGTGCAAAGC AGCCGGAACA ATCTGTGGTC 
TCATTGGCTT TGGGTGCTTT GGTGCATCTA ATTGAAGTGG GTGGACACCA GTTCAGCGAG 
GATGACTGGG ACACATTGTT AAAAAGTATT AGAGATGCTT CATACACAAC TCAACCACTT 
GAATTACTTA ATGCTTTGGG TTTTGAGAAC CCAAGCCATG ATGAGCTGAA TATTGTTGAT 
GATGGTTCCT TGAAGTGGTC TAGCCAGCAA GAAGCAAAGA ACCACCATAT TGATGTAAAT 
GAGCATGGAA AGGTATCTCC TGTTCCCTCA CCAAGGGTTG CAGAAATCAT TACTCGGAGT 
CCCATTGCAG AGTCTGGGTT ACAGATTACC ACAGATGAGT CTGCTGAAGG TATTCCATCG 
CCGTCAACAA GAGCTACAAG AGCTGCTGAA GCTGCAAATC TACAGCGGAG CCAAACAATA 
GGCCAACGAA TTATGGGAAA TATGATGGAC AATATCTTTG TTAGAAGTCT AACTTCAAAA 
TCTAAGGGAC GTGCATCAGA TGCATCTGTG CCATCTTCTC CAATAAGGCT TCCTCCTGAC 
ACTGTGGATC CTGAAGTGAA AGATGATGAG GAAAGTCCAC TATTGGGGAT TGTTCGAGGC 
AAATGCATTA CACAACTACT ACTTCTTGGT GTCATTGATG GAATTCAGAA GAAATACTGG 
GTCAAGCTGA GTGCACCACA AAAGATTGCC ATAATGGACA TTTTGTTATC TTTGCTGGAG 
TTCTCTGCTA CATATAACTC ATACAACAAT CTCAGACAGC GCATGAATCA TATTCCTGAT 
GAGAGGCCTC CACTGAATCT TCTTCGTCAG GAGTTAGCGG GAACTTCAAT TTATCTAGAC 
ATCTTACTTA AAGCAACTTC AGGATTTAAT ACGATTGAAG CAGAACAAGA AAAAATTGCT 
GATAGTCTGG AAGTTGATTC TGAATCACCC AAAGATGATT TAACTTCCAT CCAGGATTCT 
AGTGCAGTAA GCAATGTTGA TGGAATAGCT GAAAACAGGC TAGTGTCATT CTGTGAACAA 
GCTCTTAGGG AGGTATCTGA CCTGCAATCG TCTGCAGTGG AAACCACGCA CATGGATGTT 
CATCGAGTTT TGGAGTTACG TTCTCCAGTG ATTGTTAAGG TGATCAAAGG CATGTGCTTC 
ATGAACAGTC AGATATTCAG AAGACATCTG AGAGAGTTTT ATCCTTTGCT TACAAAACTT 
GTATGCTGTG ACCAGATTGA TATTCGTGGA GCACTTGGTG ATCTCTTCAA GATACAATTG 
AAAGCACTAT TGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_011652509.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650811.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCu59283
Clone ID Related Accession (Same CDS sequence)	XM_011652509.2 , XM_011652509.1
Accession Version	XM_011652509.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTACT GGTATTTCGC ACCCTTTGCA AGTGCTGTGT TACAGATGGG AATGAAGGAG 
GATACTGATG AAGTCACAAC CAAGACTCGC ATTTTATCTC TTGAGCTTCT GCAGGGTTTA 
TTAGAAGGGG TTAGCCAGAC CTTTACAAAA GACTTCCATT TTATTGACTC AGTGAAAGCA 
TATCTATCAT ATGCTTTACT GCGAGCTTCT GTCTCACAAC CTCCTGTAAT ATTTCAGTAC 
GCAACTGGAA TATTCTCTGT GCTATTGTTG CGATTCAGGG AAAGTCTTAA AGGTGAAATT 
GGCATATTCT TTCCCTTGAT AGTTCTGAGA TCATTGGATG GCACAGATTT TCCTGTTAAT 
CAAAAAACAA GTGTTTTGAA GATGCTTGAG AAAATATGTA GGGAACCTCA AATCCTTGTT 
GACATATTTG TCAACTATGA CTGTGATCTG GAGGCTCCAA ATTTGTTTGA ACGCATGGTA 
ACCACTTTAT CTAAACTTTC TCAAGGCACC CAAAATGCAG ATCCAAATTT GGCAGCTTTA 
TCCCAAGCAA CTTCAATTAA AGGATCGTCA CTTCAATGCC TTGTGAATGT GCTTAAATCA 
TTAGTTGACT GGGAGAAGTC ACGTCTTCAT TCAGAAAAGG AGGGCTTGGT TCATTCGTCT 
GAAGAAGAGT CTTCAGGCAA TGAAAATCTT GAGGTAAAGA GCAGGGAAGA TGTGACAGGT 
AATTTTGAGA AGGCAAAAGC TCACAAGTCC ACGGTGGAAG CTGCCATCTC GGAGTTCAAT 
AGGAAACCAG TAAAGGGTGT CGAGTATCTG ATTTCAAATA AGTTGGTGGA GAATACACCA 
TCTTCAGTTG CCCTCTTTCT CCGAAATACT CCCAGTTTGG ACAAGACTAT GATTGGCGAT 
TATCTTGGTC AGCATGAAGA GTTTCCTGTT GCAGTCATGC ATGCATATGT AGATTCGATG 
AAATTTTCCG GCATGAAGTT TGATGCTGCA ATTCGTGAAT TTCTCAAAGG TTTCCGTCTT 
CCAGGGGAAG CTCAAAAAAT TGATCGCATC ATGGAAAAAT TTGCAGAACG ATATTGTGCA 
GACAACCCAG GCCTTTTCAA GAATGCAGAC ACTGCATATG TTCTAGCTTA TGCAGTGATT 
ATGTTGAACA CCGATGCCCA TAACCCTATG GTGTGGCCTA AGATGTCGAA GTCCGATTTC 
ACTCGCATGA ATGTCATGAA CGATCCAGAA GATTGTGCCC CTACAGAACT CCTGGAAGAG 
ATATATGATT CTATAGTTAA AGAAGAAATC AAAATGAAAG ATGACCTTCT TGACAAAGCA 
AAAAGCCGCA GGCTGGAGGT TGAAGAGAAG GGAGGCCTTG TGAGTATTCT TAACTTGGCT 
CTTCCAAGAA GAAAGTCCTC TACTGAAGCC CAATCCGAGA GTGAGGCTAT TATTAAGCAA 
ACACAAGTTA TATTCCGAAA TCAAGGAGCA AAGAGAGGCG TTTTTTATAC CTCACAGAGA 
ATTGAACTTG TAAGGCCTAT GGTTGAGGCT GTAGGATGGC CTTTACTTGC TACTTTCTCC 
GTTACCATGG AGGAAGGTGA TAATAAACCT AGGGTTGTTC TTTGTATGGA GGGGTTTAGA 
GCTGGTATAC ATATTACACA TGTTCTTGGA ATGGATACCA TGCGCTATGC ATTTCTAACA 
TCTCTCGTCA GGTTTACTTT CTTGCATGCG CCAAAGGAAA TGCGTAGTAA AAATGTAGAA 
GCATTACGCA CGCTATTGGC TCTGTGCGAT TTAGAGACAG AATCCCTTCA AGACACTTGG 
AATGCAGTTT TAGAATGTGT GTCCCGTCTC GAATTCATCA CATCAACACC TTCAATTGCA 
GCCACTGTCA TGTATGGATC AAATCAGATT TCCAGAGATG CTGTTGTTCA ATCCCTTAGA 
GAGTTGGCTG GGAAGCCTGC AGATCAAGTA TTTGTGAACA GTGTCAAACT TCCCAGTGAC 
TCTGTTGTGG AGTTCTTCAC TGCTCTATGT GGTGTCTCTG CTGAAGAACT GAAACAAACA 
CCTGCACGTG TTTTCAGCTT GCAAAAGCTT GTTGAGATAA GCTATTATAA TATGGCTCGT 
ATACGAATGG TTTGGGCTAG GATATGGTCT GTATTGTCAA ATCACTTTAT TTCTGCTGGG 
AGCCATCATG ATGAGAAGAT TGCCATGTAT GCTATAGATT CTCTGAGGCA GCTTGGGATG 
AAGTACTTGG AGAGAGCTGA ACTTGCCAAC TTCACATTCC AAAATGACAT TCTTAAGCCA 
TTTGTTGTTC TCATGCGTAA CAGTCAAAGT GAATCTATAA GGAGCCTCAT TGTTGACTGC 
ATAGTTCAAA TGATAAAATC AAAAGTTGGT AACATAAAGT CCGGATGGCG AAGTGTTTTC 
ATGATTTTCA CTGCTTCCGC AGATGATGAA TCTGAGTCAA TTGTTGAAAG TGCCTTTGAA 
AATGTTGAAC AGGTTATATT GGAGCACTTT GATCAGGTGG TTGGGGACTG TTTTATGGAC 
TGTGTCAACT GTCTTATAAG GTTCGCCAAT AACAAAAGTT CCCATCGTAT AAGCTTGAAA 
GCTATTGCAC TCCTACGTAT TTGTGAGGAT CGATTGGCAG AGGGCCTCAT ACCCGGTGGT 
GCTTTGAAGC CGATCCACGA CAACGAGAGT GCTGAGCCAG CATTTGATAT GACTGAGCAC 
TATTGGTTCC CAATGCTTGC TGGGTTGTCA GATTTGACAT CAGACCCAAG ACCAGAGGTC 
AGAAGTTGTG CATTGGAGGT TTTGTTTGAC TTGCTGAATG AGAGAGGTTC CAAGTTCTCG 
ATGTCATTTT GGGAGAGCAT TTTTCATCGT GTCTTGTTTC CAATATTTGA TCATTTGAGA 
CATGCTGGGA AGGAAAGTGT GAATTCTAGT GGTGATGAGT GGCTTCGTGA AACAAGTATA 
CACTCGCTTC AGTTGCTTTG CAATCTCTTC AATACATTTT ACAAGGAGGT GTGCTTTATG 
CTACCTCCAC TGTTGAGCTT GCTATTAGAT TGTGCAAAGC AGCCGGAACA ATCTGTGGTC 
TCATTGGCTT TGGGTGCTTT GGTGCATCTA ATTGAAGTGG GTGGACACCA GTTCAGCGAG 
GATGACTGGG ACACATTGTT AAAAAGTATT AGAGATGCTT CATACACAAC TCAACCACTT 
GAATTACTTA ATGCTTTGGG TTTTGAGAAC CCAAGCCATG ATGAGCTGAA TATTGTTGAT 
GATGGTTCCT TGAAGTGGTC TAGCCAGCAA GAAGCAAAGA ACCACCATAT TGATGTAAAT 
GAGCATGGAA AGGTATCTCC TGTTCCCTCA CCAAGGGTTG CAGAAATCAT TACTCGGAGT 
CCCATTGCAG AGTCTGGGTT ACAGATTACC ACAGATGAGT CTGCTGAAGG TATTCCATCG 
CCGTCAACAA GAGCTACAAG AGCTGCTGAA GCTGCAAATC TACAGCGGAG CCAAACAATA 
GGCCAACGAA TTATGGGAAA TATGATGGAC AATATCTTTG TTAGAAGTCT AACTTCAAAA 
TCTAAGGGAC GTGCATCAGA TGCATCTGTG CCATCTTCTC CAATAAGGCT TCCTCCTGAC 
ACTGTGGATC CTGAAGTGAA AGATGATGAG GAAAGTCCAC TATTGGGGAT TGTTCGAGGC 
AAATGCATTA CACAACTACT ACTTCTTGGT GTCATTGATG GAATTCAGAA GAAATACTGG 
GTCAAGCTGA GTGCACCACA AAAGATTGCC ATAATGGACA TTTTGTTATC TTTGCTGGAG 
TTCTCTGCTA CATATAACTC ATACAACAAT CTCAGACAGC GCATGAATCA TATTCCTGAT 
GAGAGGCCTC CACTGAATCT TCTTCGTCAG GAGTTAGCGG GAACTTCAAT TTATCTAGAC 
ATCTTACTTA AAGCAACTTC AGGATTTAAT ACGATTGAAG CAGAACAAGA AAAAATTGCT 
GATAGTCTGG AAGTTGATTC TGAATCACCC AAAGATGATT TAACTTCCAT CCAGGATTCT 
AGTGCAGTAA GCAATGTTGA TGGAATAGCT GAAAACAGGC TAGTGTCATT CTGTGAACAA 
GCTCTTAGGG AGGTATCTGA CCTGCAATCG TCTGCAGTGG AAACCACGCA CATGGATGTT 
CATCGAGTTT TGGAGTTACG TTCTCCAGTG ATTGTTAAGG TGATCAAAGG CATGTGCTTC 
ATGAACAGTC AGATATTCAG AAGACATCTG AGAGAGTTTT ATCCTTTGCT TACAAAACTT 
GTATGCTGTG ACCAGATTGA TATTCGTGGA GCACTTGGTG ATCTCTTCAA GATACAATTG 
AAAGCACTAT TGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650811.1
CDS	98..4435
Translation	MLYWYFAPFASAVLQMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652509.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652509.1

ORF Insert Sequence:

ATGCTCTACT GGTATTTCGC ACCCTTTGCA AGTGCTGTGT TACAGATGGG AATGAAGGAG 
GATACTGATG AAGTCACAAC CAAGACTCGC ATTTTATCTC TTGAGCTTCT GCAGGGTTTA 
TTAGAAGGGG TTAGCCAGAC CTTTACAAAA GACTTCCATT TTATTGACTC AGTGAAAGCA 
TATCTATCAT ATGCTTTACT GCGAGCTTCT GTCTCACAAC CTCCTGTAAT ATTTCAGTAC 
GCAACTGGAA TATTCTCTGT GCTATTGTTG CGATTCAGGG AAAGTCTTAA AGGTGAAATT 
GGCATATTCT TTCCCTTGAT AGTTCTGAGA TCATTGGATG GCACAGATTT TCCTGTTAAT 
CAAAAAACAA GTGTTTTGAA GATGCTTGAG AAAATATGTA GGGAACCTCA AATCCTTGTT 
GACATATTTG TCAACTATGA CTGTGATCTG GAGGCTCCAA ATTTGTTTGA ACGCATGGTA 
ACCACTTTAT CTAAACTTTC TCAAGGCACC CAAAATGCAG ATCCAAATTT GGCAGCTTTA 
TCCCAAGCAA CTTCAATTAA AGGATCGTCA CTTCAATGCC TTGTGAATGT GCTTAAATCA 
TTAGTTGACT GGGAGAAGTC ACGTCTTCAT TCAGAAAAGG AGGGCTTGGT TCATTCGTCT 
GAAGAAGAGT CTTCAGGCAA TGAAAATCTT GAGGTAAAGA GCAGGGAAGA TGTGACAGGT 
AATTTTGAGA AGGCAAAAGC TCACAAGTCC ACGGTGGAAG CTGCCATCTC GGAGTTCAAT 
AGGAAACCAG TAAAGGGTGT CGAGTATCTG ATTTCAAATA AGTTGGTGGA GAATACACCA 
TCTTCAGTTG CCCTCTTTCT CCGAAATACT CCCAGTTTGG ACAAGACTAT GATTGGCGAT 
TATCTTGGTC AGCATGAAGA GTTTCCTGTT GCAGTCATGC ATGCATATGT AGATTCGATG 
AAATTTTCCG GCATGAAGTT TGATGCTGCA ATTCGTGAAT TTCTCAAAGG TTTCCGTCTT 
CCAGGGGAAG CTCAAAAAAT TGATCGCATC ATGGAAAAAT TTGCAGAACG ATATTGTGCA 
GACAACCCAG GCCTTTTCAA GAATGCAGAC ACTGCATATG TTCTAGCTTA TGCAGTGATT 
ATGTTGAACA CCGATGCCCA TAACCCTATG GTGTGGCCTA AGATGTCGAA GTCCGATTTC 
ACTCGCATGA ATGTCATGAA CGATCCAGAA GATTGTGCCC CTACAGAACT CCTGGAAGAG 
ATATATGATT CTATAGTTAA AGAAGAAATC AAAATGAAAG ATGACCTTCT TGACAAAGCA 
AAAAGCCGCA GGCTGGAGGT TGAAGAGAAG GGAGGCCTTG TGAGTATTCT TAACTTGGCT 
CTTCCAAGAA GAAAGTCCTC TACTGAAGCC CAATCCGAGA GTGAGGCTAT TATTAAGCAA 
ACACAAGTTA TATTCCGAAA TCAAGGAGCA AAGAGAGGCG TTTTTTATAC CTCACAGAGA 
ATTGAACTTG TAAGGCCTAT GGTTGAGGCT GTAGGATGGC CTTTACTTGC TACTTTCTCC 
GTTACCATGG AGGAAGGTGA TAATAAACCT AGGGTTGTTC TTTGTATGGA GGGGTTTAGA 
GCTGGTATAC ATATTACACA TGTTCTTGGA ATGGATACCA TGCGCTATGC ATTTCTAACA 
TCTCTCGTCA GGTTTACTTT CTTGCATGCG CCAAAGGAAA TGCGTAGTAA AAATGTAGAA 
GCATTACGCA CGCTATTGGC TCTGTGCGAT TTAGAGACAG AATCCCTTCA AGACACTTGG 
AATGCAGTTT TAGAATGTGT GTCCCGTCTC GAATTCATCA CATCAACACC TTCAATTGCA 
GCCACTGTCA TGTATGGATC AAATCAGATT TCCAGAGATG CTGTTGTTCA ATCCCTTAGA 
GAGTTGGCTG GGAAGCCTGC AGATCAAGTA TTTGTGAACA GTGTCAAACT TCCCAGTGAC 
TCTGTTGTGG AGTTCTTCAC TGCTCTATGT GGTGTCTCTG CTGAAGAACT GAAACAAACA 
CCTGCACGTG TTTTCAGCTT GCAAAAGCTT GTTGAGATAA GCTATTATAA TATGGCTCGT 
ATACGAATGG TTTGGGCTAG GATATGGTCT GTATTGTCAA ATCACTTTAT TTCTGCTGGG 
AGCCATCATG ATGAGAAGAT TGCCATGTAT GCTATAGATT CTCTGAGGCA GCTTGGGATG 
AAGTACTTGG AGAGAGCTGA ACTTGCCAAC TTCACATTCC AAAATGACAT TCTTAAGCCA 
TTTGTTGTTC TCATGCGTAA CAGTCAAAGT GAATCTATAA GGAGCCTCAT TGTTGACTGC 
ATAGTTCAAA TGATAAAATC AAAAGTTGGT AACATAAAGT CCGGATGGCG AAGTGTTTTC 
ATGATTTTCA CTGCTTCCGC AGATGATGAA TCTGAGTCAA TTGTTGAAAG TGCCTTTGAA 
AATGTTGAAC AGGTTATATT GGAGCACTTT GATCAGGTGG TTGGGGACTG TTTTATGGAC 
TGTGTCAACT GTCTTATAAG GTTCGCCAAT AACAAAAGTT CCCATCGTAT AAGCTTGAAA 
GCTATTGCAC TCCTACGTAT TTGTGAGGAT CGATTGGCAG AGGGCCTCAT ACCCGGTGGT 
GCTTTGAAGC CGATCCACGA CAACGAGAGT GCTGAGCCAG CATTTGATAT GACTGAGCAC 
TATTGGTTCC CAATGCTTGC TGGGTTGTCA GATTTGACAT CAGACCCAAG ACCAGAGGTC 
AGAAGTTGTG CATTGGAGGT TTTGTTTGAC TTGCTGAATG AGAGAGGTTC CAAGTTCTCG 
ATGTCATTTT GGGAGAGCAT TTTTCATCGT GTCTTGTTTC CAATATTTGA TCATTTGAGA 
CATGCTGGGA AGGAAAGTGT GAATTCTAGT GGTGATGAGT GGCTTCGTGA AACAAGTATA 
CACTCGCTTC AGTTGCTTTG CAATCTCTTC AATACATTTT ACAAGGAGGT GTGCTTTATG 
CTACCTCCAC TGTTGAGCTT GCTATTAGAT TGTGCAAAGC AGCCGGAACA ATCTGTGGTC 
TCATTGGCTT TGGGTGCTTT GGTGCATCTA ATTGAAGTGG GTGGACACCA GTTCAGCGAG 
GATGACTGGG ACACATTGTT AAAAAGTATT AGAGATGCTT CATACACAAC TCAACCACTT 
GAATTACTTA ATGCTTTGGG TTTTGAGAAC CCAAGCCATG ATGAGCTGAA TATTGTTGAT 
GATGGTTCCT TGAAGTGGTC TAGCCAGCAA GAAGCAAAGA ACCACCATAT TGATGTAAAT 
GAGCATGGAA AGGTATCTCC TGTTCCCTCA CCAAGGGTTG CAGAAATCAT TACTCGGAGT 
CCCATTGCAG AGTCTGGGTT ACAGATTACC ACAGATGAGT CTGCTGAAGG TATTCCATCG 
CCGTCAACAA GAGCTACAAG AGCTGCTGAA GCTGCAAATC TACAGCGGAG CCAAACAATA 
GGCCAACGAA TTATGGGAAA TATGATGGAC AATATCTTTG TTAGAAGTCT AACTTCAAAA 
TCTAAGGGAC GTGCATCAGA TGCATCTGTG CCATCTTCTC CAATAAGGCT TCCTCCTGAC 
ACTGTGGATC CTGAAGTGAA AGATGATGAG GAAAGTCCAC TATTGGGGAT TGTTCGAGGC 
AAATGCATTA CACAACTACT ACTTCTTGGT GTCATTGATG GAATTCAGAA GAAATACTGG 
GTCAAGCTGA GTGCACCACA AAAGATTGCC ATAATGGACA TTTTGTTATC TTTGCTGGAG 
TTCTCTGCTA CATATAACTC ATACAACAAT CTCAGACAGC GCATGAATCA TATTCCTGAT 
GAGAGGCCTC CACTGAATCT TCTTCGTCAG GAGTTAGCGG GAACTTCAAT TTATCTAGAC 
ATCTTACTTA AAGCAACTTC AGGATTTAAT ACGATTGAAG CAGAACAAGA AAAAATTGCT 
GATAGTCTGG AAGTTGATTC TGAATCACCC AAAGATGATT TAACTTCCAT CCAGGATTCT 
AGTGCAGTAA GCAATGTTGA TGGAATAGCT GAAAACAGGC TAGTGTCATT CTGTGAACAA 
GCTCTTAGGG AGGTATCTGA CCTGCAATCG TCTGCAGTGG AAACCACGCA CATGGATGTT 
CATCGAGTTT TGGAGTTACG TTCTCCAGTG ATTGTTAAGG TGATCAAAGG CATGTGCTTC 
ATGAACAGTC AGATATTCAG AAGACATCTG AGAGAGTTTT ATCCTTTGCT TACAAAACTT 
GTATGCTGTG ACCAGATTGA TATTCGTGGA GCACTTGGTG ATCTCTTCAA GATACAATTG 
AAAGCACTAT TGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_011652507.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650809.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_011652507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011652507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650809.1
CDS	256..5607
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652507.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652507.2

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_004134305.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004134353.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_004134305.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004134305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004134353.1
CDS	182..5533
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_004134305.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004134305.2

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_011652508.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650810.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_011652508.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650810.1
CDS	257..5608
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652508.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652508.1

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_004134305.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004134353.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_004134305.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004134305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004134353.1
CDS	230..5581
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_004134305.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004134305.3

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_031881971.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_031737831.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_031881971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031737831.1
CDS	233..5584
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_031881971.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031881971.1

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_011652507.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650809.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_011652507.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650809.1
CDS	243..5594
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652507.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652507.1

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218826 ( XM_011652508.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650810.1 Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.

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CloneID	OCu59282
Clone ID Related Accession (Same CDS sequence)	XM_011652507.2 , XM_004134305.2 , XM_011652508.1 , XM_004134305.3 , XM_031881971.1 , XM_011652507.1 , XM_011652508.2
Accession Version	XM_011652508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011652508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650810.1
CDS	254..5605
Translation	MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAGDTSETGGEADESQTAQSAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRLAFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEELQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSLVDWEKSRLHSEKEGLVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKAKSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVNEHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRGKCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPDERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDSSAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALLP

Target ORF information:

RefSeq Version	XM_011652508.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus brefeldin A-inhibited guanine nucleotide-exchange protein 5 (LOC101218826), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652508.2

ORF Insert Sequence:

ATGGCTGCTG GTGGTTTTGT TACGCGGGCT TTCGAGTCCA TGCTTAAAGA ATGCTCTGGC 
GGCAAGAAGT ATCCGGCTCT TCAGAAAGCT ATTCAGGCAT TTCTAGATGC TACGAAGGAG 
GTCAATCGGA GTCAACAAGC TACACCCATC GAGACAAATC AACCGGCAGC TTCAGCTGGA 
GATACCAGTG AAACTGGAGG GGAGGCAGAT GAGTCTCAGA CAGCACAATC TGCACAGGAG 
GTTGAGAATA ATGGCAAGAA AGCTGCTCCG CGGGAGCATA TATCAATTGT GTTAGCAAAT 
GCTGGCCATG TCTTGCATGG AGATGATGCA GAGCTTGTTT TGAGTCCACT TCGCCTCGCA 
TTTGACACAA AGCATTTGAA AGTCCTAGAA CTTGCTTTGG ATTGTCTCCA TAAACTCATT 
GCTTACGATC ACTTAGAAGG TGATCCTGGT TTAGAAGGTG GTAAAAATGT CTCTCTTTTC 
ACTGACATTC TGAACATGAT TTGCGGTTGT ATTGACAATT CATCGCCTGA TAGTACAATT 
TTGCAAGTGT TGAAGGTTCT TCTTACTGCA GTTGCATCTG CAAAGTTCAG AGTACACGGG 
GAACCTTTGT TAGGAGTCAT TAGGGTTTGC TACAACATTG CTCTAAATAG CAAAAGTCCC 
ATAAATCAAG CTACTTCAAA AGCAATGTTG ACTCAGATGA TCAGCATTAT ATTTAGGAGA 
ATGGAAACCG ACCAGGTTAG TTTATCTACT TCCTCTGGAA CAAAAGATTC TTCCTCTGCA 
GAAGTTTCCA GTGTGGTAGA TGAAGAAACT ACTGTGAATG AAGAAAATGA CAAAGAGACA 
ACTCTTGGAG ATGCACTCAA TTCAGTTAAA GACACATCTA TTGCATCAGT AGAGGAGCTT 
CAGAATCTTG CAGGTGGTGC TGATATCAAG GGTCTAGAGG CTGTTCTCGA CAAAGCTGTT 
CATATTGAAG ATGGTAAAAA GATGTCACGA GGGATTGATC TGGAGAGTGT GAATATTATT 
CAACGTGATG CTCTACTGGT ATTTCGCACC CTTTGCAAGA TGGGAATGAA GGAGGATACT 
GATGAAGTCA CAACCAAGAC TCGCATTTTA TCTCTTGAGC TTCTGCAGGG TTTATTAGAA 
GGGGTTAGCC AGACCTTTAC AAAAGACTTC CATTTTATTG ACTCAGTGAA AGCATATCTA 
TCATATGCTT TACTGCGAGC TTCTGTCTCA CAACCTCCTG TAATATTTCA GTACGCAACT 
GGAATATTCT CTGTGCTATT GTTGCGATTC AGGGAAAGTC TTAAAGGTGA AATTGGCATA 
TTCTTTCCCT TGATAGTTCT GAGATCATTG GATGGCACAG ATTTTCCTGT TAATCAAAAA 
ACAAGTGTTT TGAAGATGCT TGAGAAAATA TGTAGGGAAC CTCAAATCCT TGTTGACATA 
TTTGTCAACT ATGACTGTGA TCTGGAGGCT CCAAATTTGT TTGAACGCAT GGTAACCACT 
TTATCTAAAC TTTCTCAAGG CACCCAAAAT GCAGATCCAA ATTTGGCAGC TTTATCCCAA 
GCAACTTCAA TTAAAGGATC GTCACTTCAA TGCCTTGTGA ATGTGCTTAA ATCATTAGTT 
GACTGGGAGA AGTCACGTCT TCATTCAGAA AAGGAGGGCT TGGTTCATTC GTCTGAAGAA 
GAGTCTTCAG GCAATGAAAA TCTTGAGGTA AAGAGCAGGG AAGATGTGAC AGGTAATTTT 
GAGAAGGCAA AAGCTCACAA GTCCACGGTG GAAGCTGCCA TCTCGGAGTT CAATAGGAAA 
CCAGTAAAGG GTGTCGAGTA TCTGATTTCA AATAAGTTGG TGGAGAATAC ACCATCTTCA 
GTTGCCCTCT TTCTCCGAAA TACTCCCAGT TTGGACAAGA CTATGATTGG CGATTATCTT 
GGTCAGCATG AAGAGTTTCC TGTTGCAGTC ATGCATGCAT ATGTAGATTC GATGAAATTT 
TCCGGCATGA AGTTTGATGC TGCAATTCGT GAATTTCTCA AAGGTTTCCG TCTTCCAGGG 
GAAGCTCAAA AAATTGATCG CATCATGGAA AAATTTGCAG AACGATATTG TGCAGACAAC 
CCAGGCCTTT TCAAGAATGC AGACACTGCA TATGTTCTAG CTTATGCAGT GATTATGTTG 
AACACCGATG CCCATAACCC TATGGTGTGG CCTAAGATGT CGAAGTCCGA TTTCACTCGC 
ATGAATGTCA TGAACGATCC AGAAGATTGT GCCCCTACAG AACTCCTGGA AGAGATATAT 
GATTCTATAG TTAAAGAAGA AATCAAAATG AAAGATGACC TTCTTGACAA AGCAAAAAGC 
CGCAGGCTGG AGGTTGAAGA GAAGGGAGGC CTTGTGAGTA TTCTTAACTT GGCTCTTCCA 
AGAAGAAAGT CCTCTACTGA AGCCCAATCC GAGAGTGAGG CTATTATTAA GCAAACACAA 
GTTATATTCC GAAATCAAGG AGCAAAGAGA GGCGTTTTTT ATACCTCACA GAGAATTGAA 
CTTGTAAGGC CTATGGTTGA GGCTGTAGGA TGGCCTTTAC TTGCTACTTT CTCCGTTACC 
ATGGAGGAAG GTGATAATAA ACCTAGGGTT GTTCTTTGTA TGGAGGGGTT TAGAGCTGGT 
ATACATATTA CACATGTTCT TGGAATGGAT ACCATGCGCT ATGCATTTCT AACATCTCTC 
GTCAGGTTTA CTTTCTTGCA TGCGCCAAAG GAAATGCGTA GTAAAAATGT AGAAGCATTA 
CGCACGCTAT TGGCTCTGTG CGATTTAGAG ACAGAATCCC TTCAAGACAC TTGGAATGCA 
GTTTTAGAAT GTGTGTCCCG TCTCGAATTC ATCACATCAA CACCTTCAAT TGCAGCCACT 
GTCATGTATG GATCAAATCA GATTTCCAGA GATGCTGTTG TTCAATCCCT TAGAGAGTTG 
GCTGGGAAGC CTGCAGATCA AGTATTTGTG AACAGTGTCA AACTTCCCAG TGACTCTGTT 
GTGGAGTTCT TCACTGCTCT ATGTGGTGTC TCTGCTGAAG AACTGAAACA AACACCTGCA 
CGTGTTTTCA GCTTGCAAAA GCTTGTTGAG ATAAGCTATT ATAATATGGC TCGTATACGA 
ATGGTTTGGG CTAGGATATG GTCTGTATTG TCAAATCACT TTATTTCTGC TGGGAGCCAT 
CATGATGAGA AGATTGCCAT GTATGCTATA GATTCTCTGA GGCAGCTTGG GATGAAGTAC 
TTGGAGAGAG CTGAACTTGC CAACTTCACA TTCCAAAATG ACATTCTTAA GCCATTTGTT 
GTTCTCATGC GTAACAGTCA AAGTGAATCT ATAAGGAGCC TCATTGTTGA CTGCATAGTT 
CAAATGATAA AATCAAAAGT TGGTAACATA AAGTCCGGAT GGCGAAGTGT TTTCATGATT 
TTCACTGCTT CCGCAGATGA TGAATCTGAG TCAATTGTTG AAAGTGCCTT TGAAAATGTT 
GAACAGGTTA TATTGGAGCA CTTTGATCAG GTGGTTGGGG ACTGTTTTAT GGACTGTGTC 
AACTGTCTTA TAAGGTTCGC CAATAACAAA AGTTCCCATC GTATAAGCTT GAAAGCTATT 
GCACTCCTAC GTATTTGTGA GGATCGATTG GCAGAGGGCC TCATACCCGG TGGTGCTTTG 
AAGCCGATCC ACGACAACGA GAGTGCTGAG CCAGCATTTG ATATGACTGA GCACTATTGG 
TTCCCAATGC TTGCTGGGTT GTCAGATTTG ACATCAGACC CAAGACCAGA GGTCAGAAGT 
TGTGCATTGG AGGTTTTGTT TGACTTGCTG AATGAGAGAG GTTCCAAGTT CTCGATGTCA 
TTTTGGGAGA GCATTTTTCA TCGTGTCTTG TTTCCAATAT TTGATCATTT GAGACATGCT 
GGGAAGGAAA GTGTGAATTC TAGTGGTGAT GAGTGGCTTC GTGAAACAAG TATACACTCG 
CTTCAGTTGC TTTGCAATCT CTTCAATACA TTTTACAAGG AGGTGTGCTT TATGCTACCT 
CCACTGTTGA GCTTGCTATT AGATTGTGCA AAGCAGCCGG AACAATCTGT GGTCTCATTG 
GCTTTGGGTG CTTTGGTGCA TCTAATTGAA GTGGGTGGAC ACCAGTTCAG CGAGGATGAC 
TGGGACACAT TGTTAAAAAG TATTAGAGAT GCTTCATACA CAACTCAACC ACTTGAATTA 
CTTAATGCTT TGGGTTTTGA GAACCCAAGC CATGATGAGC TGAATATTGT TGATGATGGT 
TCCTTGAAGT GGTCTAGCCA GCAAGAAGCA AAGAACCACC ATATTGATGT AAATGAGCAT 
GGAAAGGTAT CTCCTGTTCC CTCACCAAGG GTTGCAGAAA TCATTACTCG GAGTCCCATT 
GCAGAGTCTG GGTTACAGAT TACCACAGAT GAGTCTGCTG AAGGTATTCC ATCGCCGTCA 
ACAAGAGCTA CAAGAGCTGC TGAAGCTGCA AATCTACAGC GGAGCCAAAC AATAGGCCAA 
CGAATTATGG GAAATATGAT GGACAATATC TTTGTTAGAA GTCTAACTTC AAAATCTAAG 
GGACGTGCAT CAGATGCATC TGTGCCATCT TCTCCAATAA GGCTTCCTCC TGACACTGTG 
GATCCTGAAG TGAAAGATGA TGAGGAAAGT CCACTATTGG GGATTGTTCG AGGCAAATGC 
ATTACACAAC TACTACTTCT TGGTGTCATT GATGGAATTC AGAAGAAATA CTGGGTCAAG 
CTGAGTGCAC CACAAAAGAT TGCCATAATG GACATTTTGT TATCTTTGCT GGAGTTCTCT 
GCTACATATA ACTCATACAA CAATCTCAGA CAGCGCATGA ATCATATTCC TGATGAGAGG 
CCTCCACTGA ATCTTCTTCG TCAGGAGTTA GCGGGAACTT CAATTTATCT AGACATCTTA 
CTTAAAGCAA CTTCAGGATT TAATACGATT GAAGCAGAAC AAGAAAAAAT TGCTGATAGT 
CTGGAAGTTG ATTCTGAATC ACCCAAAGAT GATTTAACTT CCATCCAGGA TTCTAGTGCA 
GTAAGCAATG TTGATGGAAT AGCTGAAAAC AGGCTAGTGT CATTCTGTGA ACAAGCTCTT 
AGGGAGGTAT CTGACCTGCA ATCGTCTGCA GTGGAAACCA CGCACATGGA TGTTCATCGA 
GTTTTGGAGT TACGTTCTCC AGTGATTGTT AAGGTGATCA AAGGCATGTG CTTCATGAAC 
AGTCAGATAT TCAGAAGACA TCTGAGAGAG TTTTATCCTT TGCTTACAAA ACTTGTATGC 
TGTGACCAGA TTGATATTCG TGGAGCACTT GGTGATCTCT TCAAGATACA ATTGAAAGCA 
CTATTGCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC101218826 cDNA ORF clone, Cucumis sativus(cucumber)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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