LOC101214895 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101214895
Entrez Gene ID	101214895
Full Name	probable leucine-rich repeat receptor-like protein kinase IMK3
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004146411.3	XP_004146459.1	probable leucine-rich repeat receptor-like protein kinase IMK3
XM_004146411.2	XP_004146459.1	probable leucine-rich repeat receptor-like protein kinase IMK3

Pathways from BioSystems
Gene Ontology

The following LOC101214895 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101214895 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu66478	XM_004146411.3 Latest version!	Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
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OCu66478	XM_004146411.2	Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101214895 ( XM_004146411.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004146459.1 Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.

Price & Availability↑

CloneID	OCu66478
Clone ID Related Accession (Same CDS sequence)	XM_004146411.3 , XM_004146411.2
Accession Version	XM_004146411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	probable leucine-rich repeat receptor-like protein kinase IMK3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026659.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004146411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAA CTCAAACTCC ATTTGATTTC AACTCTTTTT CGAGATACCC ATTTGGGGAT 
TTTGGGGTTT TCTTTACCCT GTGTCTTGAT GTGCTCTGTT TTTCACCTAA GAAGAAGGAG 
AAATGGAAGA ATCTGAGACA AAACCCTAAC GTGTTTGTTT TGTTGCTTTT GTTGCTTAAT 
TTGGTTCCTG TTCTGAGTCA GGATTGGGAT GGTGTGGTTG TGACTCAAGC TGATTTCCAA 
TCCCTTCAGG CATTTAAGCA AGAGCTTGAT GATCCCAAAG GGTTTTTGAA GAGTTGGAAT 
GATAGTGGAT TTGGGGCTTG TTCTGGTGGT TGGGCTGGGA TTAAATGTGC CAAGGGACAA 
GTTATTGTAA TACAGCTTCC ATGGAAGGGA TTAGGAGGTA GAATCACTGA GAAGATTGGC 
CAACTTCAAG CACTTAGGAA GCTTAGTTTA CATGATAACT CTATTGGTGG ATCAATCCCT 
TCTTCATTGG GGCTTCTTCC AAATCTTAGA GGGGTTCAGT TATTCAATAA TCGACTTTCG 
GGTTCGATTC CGGCTTCATT GGGACTTTGT CCCGTACTTC AAACACTTCA CATTAGTAAC 
AATTTGCTCA CTGGAACAAT ACCACCTACT TTGGCTAACT CCACCAAGCT TTATTGGCTT 
AACTTGAGCT TGAACTCGCT TTCGGGTCCG ATTCCAACTA CTCTTACTCG TTCGGTGTCC 
CTTACTTTCC TTGACCTTCA ACATAACAAT CTCTCAGGCA GCATTCCAGA CTCTTGGGGT 
GGTGACGAGC AAAACCGTGT GTTTCAGCTC AAGTCTCTGA CTCTTGATGG CAATCTCCTC 
TCAGGTACCA TACCAACTTC TTTAAGTAAG TTAAGTGAGC TTCAAGTGAT TTCCCTTAGC 
CACAATAGGT TAAATGGTGG CATTCCTGAA GAAATTAGTA GGCTTTCTCT GCTCAAAACA 
TTGGATGTTT CTAATAATTT CCTTAATGGA AGTATGCCCC AAAGTTTTGA TAGATTGAGA 
AACCTCTCTA TACTCAACTT GAGTAGAAAC AGATTCAATG GTCAAATTCC TGAAACTTTA 
GGGAACGTTT CAACACTTAA ACAACTTGAT TTATCTCAGA ACAATCTAAG TGGAGAAATT 
CCAGCTTCCC TTGCTGATTT GCAAGGTCTT CAATCCTTAA ATGTCTCATA CAACAACCTT 
TCGGGTTCGG TTCCTCGAGC TCTCGCTGAA AAATTCAATG CAAGTTCCTT TGTTGGGAAC 
CTTCAGCTCT GTGGGTTCAG TGGTTCAATT CTGTGCCCGT CCCCAGCCCC TTCTCAAGAA 
GCTCCTGCTC CTCCACCAGA AAGTTCGACA ACCCGACATC GGAAGTTAAG TACTAAAGAT 
ATAATCCTCA TAGCAGCAGG AGCCCTCCTT CTGGTATTGG TTATAGTTTT CTTTATATTG 
CTCTGCTGCT TGATAAGGAA AAGGGCTGCT TCAAAAGGGA AGGATGGTGG TGAAGCTGGA 
GCAGCTGGGG CTGCGAGGGC CGAGAAGGGC GTCCCCCCGA CTAGCAGTGA AGTTGAAGCG 
GCTGGAGGTG GGGATGCTGG TGGGAAGCTC GTCCATTTCG ACGGGCAAAC GGTCTTCACA 
GCAGATGATC TTTTGTGTGC AACAGCAGAA ATAATGGGAA AGAGTACTTA TGGGACTGTT 
TACAAGGCCA CGTTGGAGGA TGGAAATCAA GTTGCGGTGA AGAGATTGAG AGAGAAAATC 
ACTAAAAGCC AAAAGGAATT TGAAGCAGAG GTCAACATAC TTGGCAAGAT CAGACATCCA 
AATCTATTAG CTTTAAGAGC TTATTACTTG GGACCCAAAG GAGAGAAGCT TCTCGTTTTC 
GATTATATGC CGAATGGAAG TCTCGCGACG TTCCTGCATG CTCGTGGACC GGACACGTCC 
ATCGATTGGC CAACAAGGAT GAAGATAGCT CAGGGTATGA CACGTGGCCT GTGTCACCTT 
CACACGCACG AGAACAGCAT CCATGGAAAC CTGACATCAA GCAACATTCT ACTAGATGAG 
TACATAAACG CTAAGATTGC AGATTTTGGC TTGTCAAGGT TGATGACAGC AGCTGCAAGC 
TCAAATGTAA TAGCAACAGC CGGGGCACTT GGGTACCGAG CACCAGAACT CTCAAAGTTG 
AAGAAAGCAA ACACAAAAAC CGATATTTAC AGCCTTGGAG TCATCATATT GGAACTCTTG 
ACTGGTAAGT CCCCCGGTGA AGCCATGAAC GGAGTTGATT TACCTCAATG GGTCGCATCG 
ATAGTCAAAG AGGAGTGGAC TAATGAAGTT TTTGATTTGG AACTGATGAG AGATGCATCT 
ACCATTGGAG ATGAGTTACT AAACACTCTG AAACTCGCTT TGCATTGTGT CGACCCTTCA 
CCGTCAGCGA GACCGGAAGT TCAACAAGTT TTGCAACAAC TGGAAGAGAT AAGGCCAGAA 
ACTGCTCCAA GTTCTGGGGA TGATGGAATA GGAATAGGAG GAGGAGGAGG AGGAGCCCCT 
TCAACAAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004146459.1
CDS	378..2912
Translation	MGKTQTPFDFNSFSRYPFGDFGVFFTLCLDVLCFSPKKKEKWKNLRQNPNVFVLLLLLLNLVPVLSQDWDGVVVTQADFQSLQAFKQELDDPKGFLKSWNDSGFGACSGGWAGIKCAKGQVIVIQLPWKGLGGRITEKIGQLQALRKLSLHDNSIGGSIPSSLGLLPNLRGVQLFNNRLSGSIPASLGLCPVLQTLHISNNLLTGTIPPTLANSTKLYWLNLSLNSLSGPIPTTLTRSVSLTFLDLQHNNLSGSIPDSWGGDEQNRVFQLKSLTLDGNLLSGTIPTSLSKLSELQVISLSHNRLNGGIPEEISRLSLLKTLDVSNNFLNGSMPQSFDRLRNLSILNLSRNRFNGQIPETLGNVSTLKQLDLSQNNLSGEIPASLADLQGLQSLNVSYNNLSGSVPRALAEKFNASSFVGNLQLCGFSGSILCPSPAPSQEAPAPPPESSTTRHRKLSTKDIILIAAGALLLVLVIVFFILLCCLIRKRAASKGKDGGEAGAAGAARAEKGVPPTSSEVEAAGGGDAGGKLVHFDGQTVFTADDLLCATAEIMGKSTYGTVYKATLEDGNQVAVKRLREKITKSQKEFEAEVNILGKIRHPNLLALRAYYLGPKGEKLLVFDYMPNGSLATFLHARGPDTSIDWPTRMKIAQGMTRGLCHLHTHENSIHGNLTSSNILLDEYINAKIADFGLSRLMTAAASSNVIATAGALGYRAPELSKLKKANTKTDIYSLGVIILELLTGKSPGEAMNGVDLPQWVASIVKEEWTNEVFDLELMRDASTIGDELLNTLKLALHCVDPSPSARPEVQQVLQQLEEIRPETAPSSGDDGIGIGGGGGGAPSTSE

Target ORF information:

RefSeq Version	XM_004146411.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004146411.3

ORF Insert Sequence:

ATGGGTAAAA CTCAAACTCC ATTTGATTTC AACTCTTTTT CGAGATACCC ATTTGGGGAT 
TTTGGGGTTT TCTTTACCCT GTGTCTTGAT GTGCTCTGTT TTTCACCTAA GAAGAAGGAG 
AAATGGAAGA ATCTGAGACA AAACCCTAAC GTGTTTGTTT TGTTGCTTTT GTTGCTTAAT 
TTGGTTCCTG TTCTGAGTCA GGATTGGGAT GGTGTGGTTG TGACTCAAGC TGATTTCCAA 
TCCCTTCAGG CATTTAAGCA AGAGCTTGAT GATCCCAAAG GGTTTTTGAA GAGTTGGAAT 
GATAGTGGAT TTGGGGCTTG TTCTGGTGGT TGGGCTGGGA TTAAATGTGC CAAGGGACAA 
GTTATTGTAA TACAGCTTCC ATGGAAGGGA TTAGGAGGTA GAATCACTGA GAAGATTGGC 
CAACTTCAAG CACTTAGGAA GCTTAGTTTA CATGATAACT CTATTGGTGG ATCAATCCCT 
TCTTCATTGG GGCTTCTTCC AAATCTTAGA GGGGTTCAGT TATTCAATAA TCGACTTTCG 
GGTTCGATTC CGGCTTCATT GGGACTTTGT CCCGTACTTC AAACACTTCA CATTAGTAAC 
AATTTGCTCA CTGGAACAAT ACCACCTACT TTGGCTAACT CCACCAAGCT TTATTGGCTT 
AACTTGAGCT TGAACTCGCT TTCGGGTCCG ATTCCAACTA CTCTTACTCG TTCGGTGTCC 
CTTACTTTCC TTGACCTTCA ACATAACAAT CTCTCAGGCA GCATTCCAGA CTCTTGGGGT 
GGTGACGAGC AAAACCGTGT GTTTCAGCTC AAGTCTCTGA CTCTTGATGG CAATCTCCTC 
TCAGGTACCA TACCAACTTC TTTAAGTAAG TTAAGTGAGC TTCAAGTGAT TTCCCTTAGC 
CACAATAGGT TAAATGGTGG CATTCCTGAA GAAATTAGTA GGCTTTCTCT GCTCAAAACA 
TTGGATGTTT CTAATAATTT CCTTAATGGA AGTATGCCCC AAAGTTTTGA TAGATTGAGA 
AACCTCTCTA TACTCAACTT GAGTAGAAAC AGATTCAATG GTCAAATTCC TGAAACTTTA 
GGGAACGTTT CAACACTTAA ACAACTTGAT TTATCTCAGA ACAATCTAAG TGGAGAAATT 
CCAGCTTCCC TTGCTGATTT GCAAGGTCTT CAATCCTTAA ATGTCTCATA CAACAACCTT 
TCGGGTTCGG TTCCTCGAGC TCTCGCTGAA AAATTCAATG CAAGTTCCTT TGTTGGGAAC 
CTTCAGCTCT GTGGGTTCAG TGGTTCAATT CTGTGCCCGT CCCCAGCCCC TTCTCAAGAA 
GCTCCTGCTC CTCCACCAGA AAGTTCGACA ACCCGACATC GGAAGTTAAG TACTAAAGAT 
ATAATCCTCA TAGCAGCAGG AGCCCTCCTT CTGGTATTGG TTATAGTTTT CTTTATATTG 
CTCTGCTGCT TGATAAGGAA AAGGGCTGCT TCAAAAGGGA AGGATGGTGG TGAAGCTGGA 
GCAGCTGGGG CTGCGAGGGC CGAGAAGGGC GTCCCCCCGA CTAGCAGTGA AGTTGAAGCG 
GCTGGAGGTG GGGATGCTGG TGGGAAGCTC GTCCATTTCG ACGGGCAAAC GGTCTTCACA 
GCAGATGATC TTTTGTGTGC AACAGCAGAA ATAATGGGAA AGAGTACTTA TGGGACTGTT 
TACAAGGCCA CGTTGGAGGA TGGAAATCAA GTTGCGGTGA AGAGATTGAG AGAGAAAATC 
ACTAAAAGCC AAAAGGAATT TGAAGCAGAG GTCAACATAC TTGGCAAGAT CAGACATCCA 
AATCTATTAG CTTTAAGAGC TTATTACTTG GGACCCAAAG GAGAGAAGCT TCTCGTTTTC 
GATTATATGC CGAATGGAAG TCTCGCGACG TTCCTGCATG CTCGTGGACC GGACACGTCC 
ATCGATTGGC CAACAAGGAT GAAGATAGCT CAGGGTATGA CACGTGGCCT GTGTCACCTT 
CACACGCACG AGAACAGCAT CCATGGAAAC CTGACATCAA GCAACATTCT ACTAGATGAG 
TACATAAACG CTAAGATTGC AGATTTTGGC TTGTCAAGGT TGATGACAGC AGCTGCAAGC 
TCAAATGTAA TAGCAACAGC CGGGGCACTT GGGTACCGAG CACCAGAACT CTCAAAGTTG 
AAGAAAGCAA ACACAAAAAC CGATATTTAC AGCCTTGGAG TCATCATATT GGAACTCTTG 
ACTGGTAAGT CCCCCGGTGA AGCCATGAAC GGAGTTGATT TACCTCAATG GGTCGCATCG 
ATAGTCAAAG AGGAGTGGAC TAATGAAGTT TTTGATTTGG AACTGATGAG AGATGCATCT 
ACCATTGGAG ATGAGTTACT AAACACTCTG AAACTCGCTT TGCATTGTGT CGACCCTTCA 
CCGTCAGCGA GACCGGAAGT TCAACAAGTT TTGCAACAAC TGGAAGAGAT AAGGCCAGAA 
ACTGCTCCAA GTTCTGGGGA TGATGGAATA GGAATAGGAG GAGGAGGAGG AGGAGCCCCT 
TCAACAAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101214895 ( XM_004146411.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004146459.1 Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.

Price & Availability↑

CloneID	OCu66478
Clone ID Related Accession (Same CDS sequence)	XM_004146411.3 , XM_004146411.2
Accession Version	XM_004146411.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	probable leucine-rich repeat receptor-like protein kinase IMK3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953807.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004146411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAA CTCAAACTCC ATTTGATTTC AACTCTTTTT CGAGATACCC ATTTGGGGAT 
TTTGGGGTTT TCTTTACCCT GTGTCTTGAT GTGCTCTGTT TTTCACCTAA GAAGAAGGAG 
AAATGGAAGA ATCTGAGACA AAACCCTAAC GTGTTTGTTT TGTTGCTTTT GTTGCTTAAT 
TTGGTTCCTG TTCTGAGTCA GGATTGGGAT GGTGTGGTTG TGACTCAAGC TGATTTCCAA 
TCCCTTCAGG CATTTAAGCA AGAGCTTGAT GATCCCAAAG GGTTTTTGAA GAGTTGGAAT 
GATAGTGGAT TTGGGGCTTG TTCTGGTGGT TGGGCTGGGA TTAAATGTGC CAAGGGACAA 
GTTATTGTAA TACAGCTTCC ATGGAAGGGA TTAGGAGGTA GAATCACTGA GAAGATTGGC 
CAACTTCAAG CACTTAGGAA GCTTAGTTTA CATGATAACT CTATTGGTGG ATCAATCCCT 
TCTTCATTGG GGCTTCTTCC AAATCTTAGA GGGGTTCAGT TATTCAATAA TCGACTTTCG 
GGTTCGATTC CGGCTTCATT GGGACTTTGT CCCGTACTTC AAACACTTCA CATTAGTAAC 
AATTTGCTCA CTGGAACAAT ACCACCTACT TTGGCTAACT CCACCAAGCT TTATTGGCTT 
AACTTGAGCT TGAACTCGCT TTCGGGTCCG ATTCCAACTA CTCTTACTCG TTCGGTGTCC 
CTTACTTTCC TTGACCTTCA ACATAACAAT CTCTCAGGCA GCATTCCAGA CTCTTGGGGT 
GGTGACGAGC AAAACCGTGT GTTTCAGCTC AAGTCTCTGA CTCTTGATGG CAATCTCCTC 
TCAGGTACCA TACCAACTTC TTTAAGTAAG TTAAGTGAGC TTCAAGTGAT TTCCCTTAGC 
CACAATAGGT TAAATGGTGG CATTCCTGAA GAAATTAGTA GGCTTTCTCT GCTCAAAACA 
TTGGATGTTT CTAATAATTT CCTTAATGGA AGTATGCCCC AAAGTTTTGA TAGATTGAGA 
AACCTCTCTA TACTCAACTT GAGTAGAAAC AGATTCAATG GTCAAATTCC TGAAACTTTA 
GGGAACGTTT CAACACTTAA ACAACTTGAT TTATCTCAGA ACAATCTAAG TGGAGAAATT 
CCAGCTTCCC TTGCTGATTT GCAAGGTCTT CAATCCTTAA ATGTCTCATA CAACAACCTT 
TCGGGTTCGG TTCCTCGAGC TCTCGCTGAA AAATTCAATG CAAGTTCCTT TGTTGGGAAC 
CTTCAGCTCT GTGGGTTCAG TGGTTCAATT CTGTGCCCGT CCCCAGCCCC TTCTCAAGAA 
GCTCCTGCTC CTCCACCAGA AAGTTCGACA ACCCGACATC GGAAGTTAAG TACTAAAGAT 
ATAATCCTCA TAGCAGCAGG AGCCCTCCTT CTGGTATTGG TTATAGTTTT CTTTATATTG 
CTCTGCTGCT TGATAAGGAA AAGGGCTGCT TCAAAAGGGA AGGATGGTGG TGAAGCTGGA 
GCAGCTGGGG CTGCGAGGGC CGAGAAGGGC GTCCCCCCGA CTAGCAGTGA AGTTGAAGCG 
GCTGGAGGTG GGGATGCTGG TGGGAAGCTC GTCCATTTCG ACGGGCAAAC GGTCTTCACA 
GCAGATGATC TTTTGTGTGC AACAGCAGAA ATAATGGGAA AGAGTACTTA TGGGACTGTT 
TACAAGGCCA CGTTGGAGGA TGGAAATCAA GTTGCGGTGA AGAGATTGAG AGAGAAAATC 
ACTAAAAGCC AAAAGGAATT TGAAGCAGAG GTCAACATAC TTGGCAAGAT CAGACATCCA 
AATCTATTAG CTTTAAGAGC TTATTACTTG GGACCCAAAG GAGAGAAGCT TCTCGTTTTC 
GATTATATGC CGAATGGAAG TCTCGCGACG TTCCTGCATG CTCGTGGACC GGACACGTCC 
ATCGATTGGC CAACAAGGAT GAAGATAGCT CAGGGTATGA CACGTGGCCT GTGTCACCTT 
CACACGCACG AGAACAGCAT CCATGGAAAC CTGACATCAA GCAACATTCT ACTAGATGAG 
TACATAAACG CTAAGATTGC AGATTTTGGC TTGTCAAGGT TGATGACAGC AGCTGCAAGC 
TCAAATGTAA TAGCAACAGC CGGGGCACTT GGGTACCGAG CACCAGAACT CTCAAAGTTG 
AAGAAAGCAA ACACAAAAAC CGATATTTAC AGCCTTGGAG TCATCATATT GGAACTCTTG 
ACTGGTAAGT CCCCCGGTGA AGCCATGAAC GGAGTTGATT TACCTCAATG GGTCGCATCG 
ATAGTCAAAG AGGAGTGGAC TAATGAAGTT TTTGATTTGG AACTGATGAG AGATGCATCT 
ACCATTGGAG ATGAGTTACT AAACACTCTG AAACTCGCTT TGCATTGTGT CGACCCTTCA 
CCGTCAGCGA GACCGGAAGT TCAACAAGTT TTGCAACAAC TGGAAGAGAT AAGGCCAGAA 
ACTGCTCCAA GTTCTGGGGA TGATGGAATA GGAATAGGAG GAGGAGGAGG AGGAGCCCCT 
TCAACAAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004146459.1
CDS	506..3040
Translation	MGKTQTPFDFNSFSRYPFGDFGVFFTLCLDVLCFSPKKKEKWKNLRQNPNVFVLLLLLLNLVPVLSQDWDGVVVTQADFQSLQAFKQELDDPKGFLKSWNDSGFGACSGGWAGIKCAKGQVIVIQLPWKGLGGRITEKIGQLQALRKLSLHDNSIGGSIPSSLGLLPNLRGVQLFNNRLSGSIPASLGLCPVLQTLHISNNLLTGTIPPTLANSTKLYWLNLSLNSLSGPIPTTLTRSVSLTFLDLQHNNLSGSIPDSWGGDEQNRVFQLKSLTLDGNLLSGTIPTSLSKLSELQVISLSHNRLNGGIPEEISRLSLLKTLDVSNNFLNGSMPQSFDRLRNLSILNLSRNRFNGQIPETLGNVSTLKQLDLSQNNLSGEIPASLADLQGLQSLNVSYNNLSGSVPRALAEKFNASSFVGNLQLCGFSGSILCPSPAPSQEAPAPPPESSTTRHRKLSTKDIILIAAGALLLVLVIVFFILLCCLIRKRAASKGKDGGEAGAAGAARAEKGVPPTSSEVEAAGGGDAGGKLVHFDGQTVFTADDLLCATAEIMGKSTYGTVYKATLEDGNQVAVKRLREKITKSQKEFEAEVNILGKIRHPNLLALRAYYLGPKGEKLLVFDYMPNGSLATFLHARGPDTSIDWPTRMKIAQGMTRGLCHLHTHENSIHGNLTSSNILLDEYINAKIADFGLSRLMTAAASSNVIATAGALGYRAPELSKLKKANTKTDIYSLGVIILELLTGKSPGEAMNGVDLPQWVASIVKEEWTNEVFDLELMRDASTIGDELLNTLKLALHCVDPSPSARPEVQQVLQQLEEIRPETAPSSGDDGIGIGGGGGGAPSTSE

Target ORF information:

RefSeq Version	XM_004146411.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable leucine-rich repeat receptor-like protein kinase IMK3 (LOC101214895), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004146411.2

ORF Insert Sequence:

ATGGGTAAAA CTCAAACTCC ATTTGATTTC AACTCTTTTT CGAGATACCC ATTTGGGGAT 
TTTGGGGTTT TCTTTACCCT GTGTCTTGAT GTGCTCTGTT TTTCACCTAA GAAGAAGGAG 
AAATGGAAGA ATCTGAGACA AAACCCTAAC GTGTTTGTTT TGTTGCTTTT GTTGCTTAAT 
TTGGTTCCTG TTCTGAGTCA GGATTGGGAT GGTGTGGTTG TGACTCAAGC TGATTTCCAA 
TCCCTTCAGG CATTTAAGCA AGAGCTTGAT GATCCCAAAG GGTTTTTGAA GAGTTGGAAT 
GATAGTGGAT TTGGGGCTTG TTCTGGTGGT TGGGCTGGGA TTAAATGTGC CAAGGGACAA 
GTTATTGTAA TACAGCTTCC ATGGAAGGGA TTAGGAGGTA GAATCACTGA GAAGATTGGC 
CAACTTCAAG CACTTAGGAA GCTTAGTTTA CATGATAACT CTATTGGTGG ATCAATCCCT 
TCTTCATTGG GGCTTCTTCC AAATCTTAGA GGGGTTCAGT TATTCAATAA TCGACTTTCG 
GGTTCGATTC CGGCTTCATT GGGACTTTGT CCCGTACTTC AAACACTTCA CATTAGTAAC 
AATTTGCTCA CTGGAACAAT ACCACCTACT TTGGCTAACT CCACCAAGCT TTATTGGCTT 
AACTTGAGCT TGAACTCGCT TTCGGGTCCG ATTCCAACTA CTCTTACTCG TTCGGTGTCC 
CTTACTTTCC TTGACCTTCA ACATAACAAT CTCTCAGGCA GCATTCCAGA CTCTTGGGGT 
GGTGACGAGC AAAACCGTGT GTTTCAGCTC AAGTCTCTGA CTCTTGATGG CAATCTCCTC 
TCAGGTACCA TACCAACTTC TTTAAGTAAG TTAAGTGAGC TTCAAGTGAT TTCCCTTAGC 
CACAATAGGT TAAATGGTGG CATTCCTGAA GAAATTAGTA GGCTTTCTCT GCTCAAAACA 
TTGGATGTTT CTAATAATTT CCTTAATGGA AGTATGCCCC AAAGTTTTGA TAGATTGAGA 
AACCTCTCTA TACTCAACTT GAGTAGAAAC AGATTCAATG GTCAAATTCC TGAAACTTTA 
GGGAACGTTT CAACACTTAA ACAACTTGAT TTATCTCAGA ACAATCTAAG TGGAGAAATT 
CCAGCTTCCC TTGCTGATTT GCAAGGTCTT CAATCCTTAA ATGTCTCATA CAACAACCTT 
TCGGGTTCGG TTCCTCGAGC TCTCGCTGAA AAATTCAATG CAAGTTCCTT TGTTGGGAAC 
CTTCAGCTCT GTGGGTTCAG TGGTTCAATT CTGTGCCCGT CCCCAGCCCC TTCTCAAGAA 
GCTCCTGCTC CTCCACCAGA AAGTTCGACA ACCCGACATC GGAAGTTAAG TACTAAAGAT 
ATAATCCTCA TAGCAGCAGG AGCCCTCCTT CTGGTATTGG TTATAGTTTT CTTTATATTG 
CTCTGCTGCT TGATAAGGAA AAGGGCTGCT TCAAAAGGGA AGGATGGTGG TGAAGCTGGA 
GCAGCTGGGG CTGCGAGGGC CGAGAAGGGC GTCCCCCCGA CTAGCAGTGA AGTTGAAGCG 
GCTGGAGGTG GGGATGCTGG TGGGAAGCTC GTCCATTTCG ACGGGCAAAC GGTCTTCACA 
GCAGATGATC TTTTGTGTGC AACAGCAGAA ATAATGGGAA AGAGTACTTA TGGGACTGTT 
TACAAGGCCA CGTTGGAGGA TGGAAATCAA GTTGCGGTGA AGAGATTGAG AGAGAAAATC 
ACTAAAAGCC AAAAGGAATT TGAAGCAGAG GTCAACATAC TTGGCAAGAT CAGACATCCA 
AATCTATTAG CTTTAAGAGC TTATTACTTG GGACCCAAAG GAGAGAAGCT TCTCGTTTTC 
GATTATATGC CGAATGGAAG TCTCGCGACG TTCCTGCATG CTCGTGGACC GGACACGTCC 
ATCGATTGGC CAACAAGGAT GAAGATAGCT CAGGGTATGA CACGTGGCCT GTGTCACCTT 
CACACGCACG AGAACAGCAT CCATGGAAAC CTGACATCAA GCAACATTCT ACTAGATGAG 
TACATAAACG CTAAGATTGC AGATTTTGGC TTGTCAAGGT TGATGACAGC AGCTGCAAGC 
TCAAATGTAA TAGCAACAGC CGGGGCACTT GGGTACCGAG CACCAGAACT CTCAAAGTTG 
AAGAAAGCAA ACACAAAAAC CGATATTTAC AGCCTTGGAG TCATCATATT GGAACTCTTG 
ACTGGTAAGT CCCCCGGTGA AGCCATGAAC GGAGTTGATT TACCTCAATG GGTCGCATCG 
ATAGTCAAAG AGGAGTGGAC TAATGAAGTT TTTGATTTGG AACTGATGAG AGATGCATCT 
ACCATTGGAG ATGAGTTACT AAACACTCTG AAACTCGCTT TGCATTGTGT CGACCCTTCA 
CCGTCAGCGA GACCGGAAGT TCAACAAGTT TTGCAACAAC TGGAAGAGAT AAGGCCAGAA 
ACTGCTCCAA GTTCTGGGGA TGATGGAATA GGAATAGGAG GAGGAGGAGG AGGAGCCCCT 
TCAACAAGTG AGTGA