DOLK cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOLK
Entrez Gene ID	104066793
Full Name	dolichol kinase
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009569456.1	XP_009567751.1	dolichol kinase

Pathways from BioSystems
Gene Ontology

The following DOLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu51747	XM_009569456.1 Latest version!	Cuculus canorus dolichol kinase (DOLK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOLK ( XM_009569456.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009567751.1 Cuculus canorus dolichol kinase (DOLK), mRNA.

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CloneID	OCu51747
Clone ID Related Accession (Same CDS sequence)	XM_009569456.1
Accession Version	XM_009569456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	dolichol kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009258105.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAACA AGCCAGTGCT GGCAGAGTCC CTGATTGTGT TCCTCATCGT TCTCTGCGTG 
CACGCAGTAG TGTGGGACCG GTATTCCTGG TGTGCCGTCG CTCTCACCAT CCAGGCTTTT 
TATGTCCAAT TCAAATGGGA CCGTCTGCTC CAGCAGGGTG GGGCTGTATT CCAGTTCCGT 
ACAGCAGCCA ACAGCGGCCT CCTGCCAGCT AGCATGGTCA TCCCCTTGCT GGGGATAGTG 
ATGAAGGAGA GGTGCAGGTC TGCTGGCATC GTGTACTTCG AACGTTTTGG CATAATTGTA 
GCTTCCACCG GCATGCTGGT TGCTCTCTTT TTATCTGTAA TAGCAGTTGG CATCACAAAA 
CCTGTGCCAA CCAACACTTG CATACTTACC GGTATTGCTG GCAGTGTCAT TATCTATACC 
ATGAAGCACT CCTTGACTGT TTCAGAAGTG ATAGAGGTCC TAGAGGTGCT GCTAATTTTT 
GTCTACCTCA GTATGGTCTT GCTGTACTTG TTACCTCGAT GTTTTACTCC CGGAGAAGCA 
TTGCTTGTTC TCGGAGGCAT CAGTTTTGTT CTCAATCAGC TCATTAAACG CTCACTAAAT 
GTAGTTGAGG GCAGGGGTGA TCCCATAGAC TTTTTCCTTC TGGTAGCAGT TGTTGGAGTT 
GTTCTTCTTG GGCTTTTTTT CACTGTGCTC TTCATTTTCA TGGACTCGGG TACGTGGATC 
TCCTCCCTGT TTTTCCACAT GATGACAGCA GTGCTAGGCT TAGGGGTCAT CATGCCTTGG 
CTGTACCGGC TGATCCAGAG GAACCCTTTG TTCTGGCTAC TCCAGTTTCT GTTTCAGACA 
CAGACAAGAG TTTACCTTCT TGTATACTGG ACCTTTTTGG CTGCTTCAGC ATGTGGCGTA 
GTTTTCTACC AGAATGCTAA GAGATCATCC GAATCTAAAA AACACCAGGC CTCGACTATA 
ACTAGGAAAT ATTTCCATTT CATTGTTGTA GCTACTTACG TTCCCGGACT AATCTATGAC 
CGCCAGCTTC TCTACGTTGC TGCAGTACTG TGTCTGGCAG CCTTTATCTT CTTAGAGTAT 
GTTCGGTACT TCAGGATCAA ACCATTTGGC CAAACCCTTA GGCATTTGCT GTCTCTCTTC 
TTGGATGAAA GAGACAGTGG ACCTCTAATC TTGACTCATA TTTATCTTCT TCTTGGCATG 
TCCCTTCCAG TATGGTTGTT CCCCAGATCT TGTGCTCCAA AAGGGACCTT GTCTGGGGCA 
GGAGCACTGG TTCCCTACTC CGGGGTGCTG GCGGTAGGGG TAGGAGACAC CATCGCCTCT 
GTTTTTGGCA GCACAATGGG GGAAATCAAG TGGCCGGGAA CAAAGAAGAC CGTTGAAGGG 
ACAATGACAG CTATTTTTGC TCAGATCATA GCTGTGGCGC TTATTCTAAT CTTTGACAGC 
AGTGTCAACC TGAACTCCAG CTATGCCTGG ATTCTGGCAT CTGTGAGTTT GGTTTCTCTT 
TTGGAAGCTT ACACTACCCA AATCGATAAT CTGCTCTTGC CTCTCTACCT CCAGATAATG 
TTTATGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009567751.1
CDS	127..1698
Translation	MLNKPVLAESLIVFLIVLCVHAVVWDRYSWCAVALTIQAFYVQFKWDRLLQQGGAVFQFRTAANSGLLPASMVIPLLGIVMKERCRSAGIVYFERFGIIVASTGMLVALFLSVIAVGITKPVPTNTCILTGIAGSVIIYTMKHSLTVSEVIEVLEVLLIFVYLSMVLLYLLPRCFTPGEALLVLGGISFVLNQLIKRSLNVVEGRGDPIDFFLLVAVVGVVLLGLFFTVLFIFMDSGTWISSLFFHMMTAVLGLGVIMPWLYRLIQRNPLFWLLQFLFQTQTRVYLLVYWTFLAASACGVVFYQNAKRSSESKKHQASTITRKYFHFIVVATYVPGLIYDRQLLYVAAVLCLAAFIFLEYVRYFRIKPFGQTLRHLLSLFLDERDSGPLILTHIYLLLGMSLPVWLFPRSCAPKGTLSGAGALVPYSGVLAVGVGDTIASVFGSTMGEIKWPGTKKTVEGTMTAIFAQIIAVALILIFDSSVNLNSSYAWILASVSLVSLLEAYTTQIDNLLLPLYLQIMFMA

Target ORF information:

RefSeq Version	XM_009569456.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus dolichol kinase (DOLK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009569456.1

ORF Insert Sequence:

ATGTTAAACA AGCCAGTGCT GGCAGAGTCC CTGATTGTGT TCCTCATCGT TCTCTGCGTG 
CACGCAGTAG TGTGGGACCG GTATTCCTGG TGTGCCGTCG CTCTCACCAT CCAGGCTTTT 
TATGTCCAAT TCAAATGGGA CCGTCTGCTC CAGCAGGGTG GGGCTGTATT CCAGTTCCGT 
ACAGCAGCCA ACAGCGGCCT CCTGCCAGCT AGCATGGTCA TCCCCTTGCT GGGGATAGTG 
ATGAAGGAGA GGTGCAGGTC TGCTGGCATC GTGTACTTCG AACGTTTTGG CATAATTGTA 
GCTTCCACCG GCATGCTGGT TGCTCTCTTT TTATCTGTAA TAGCAGTTGG CATCACAAAA 
CCTGTGCCAA CCAACACTTG CATACTTACC GGTATTGCTG GCAGTGTCAT TATCTATACC 
ATGAAGCACT CCTTGACTGT TTCAGAAGTG ATAGAGGTCC TAGAGGTGCT GCTAATTTTT 
GTCTACCTCA GTATGGTCTT GCTGTACTTG TTACCTCGAT GTTTTACTCC CGGAGAAGCA 
TTGCTTGTTC TCGGAGGCAT CAGTTTTGTT CTCAATCAGC TCATTAAACG CTCACTAAAT 
GTAGTTGAGG GCAGGGGTGA TCCCATAGAC TTTTTCCTTC TGGTAGCAGT TGTTGGAGTT 
GTTCTTCTTG GGCTTTTTTT CACTGTGCTC TTCATTTTCA TGGACTCGGG TACGTGGATC 
TCCTCCCTGT TTTTCCACAT GATGACAGCA GTGCTAGGCT TAGGGGTCAT CATGCCTTGG 
CTGTACCGGC TGATCCAGAG GAACCCTTTG TTCTGGCTAC TCCAGTTTCT GTTTCAGACA 
CAGACAAGAG TTTACCTTCT TGTATACTGG ACCTTTTTGG CTGCTTCAGC ATGTGGCGTA 
GTTTTCTACC AGAATGCTAA GAGATCATCC GAATCTAAAA AACACCAGGC CTCGACTATA 
ACTAGGAAAT ATTTCCATTT CATTGTTGTA GCTACTTACG TTCCCGGACT AATCTATGAC 
CGCCAGCTTC TCTACGTTGC TGCAGTACTG TGTCTGGCAG CCTTTATCTT CTTAGAGTAT 
GTTCGGTACT TCAGGATCAA ACCATTTGGC CAAACCCTTA GGCATTTGCT GTCTCTCTTC 
TTGGATGAAA GAGACAGTGG ACCTCTAATC TTGACTCATA TTTATCTTCT TCTTGGCATG 
TCCCTTCCAG TATGGTTGTT CCCCAGATCT TGTGCTCCAA AAGGGACCTT GTCTGGGGCA 
GGAGCACTGG TTCCCTACTC CGGGGTGCTG GCGGTAGGGG TAGGAGACAC CATCGCCTCT 
GTTTTTGGCA GCACAATGGG GGAAATCAAG TGGCCGGGAA CAAAGAAGAC CGTTGAAGGG 
ACAATGACAG CTATTTTTGC TCAGATCATA GCTGTGGCGC TTATTCTAAT CTTTGACAGC 
AGTGTCAACC TGAACTCCAG CTATGCCTGG ATTCTGGCAT CTGTGAGTTT GGTTTCTCTT 
TTGGAAGCTT ACACTACCCA AATCGATAAT CTGCTCTTGC CTCTCTACCT CCAGATAATG 
TTTATGGCAT AA