MME cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	MME
Entrez Gene ID	104065231
Full Name	membrane metalloendopeptidase
Synonyms	Neprilysin
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009567675.1	XP_009565970.1	neprilysin

Pathways from BioSystems
Gene Ontology

The following MME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu50306	XM_009567675.1 Latest version!	Cuculus canorus membrane metallo-endopeptidase (MME), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MME ( XM_009567675.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009565970.1 Cuculus canorus membrane metallo-endopeptidase (MME), mRNA.

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CloneID	OCu50306
Clone ID Related Accession (Same CDS sequence)	XM_009567675.1
Accession Version	XM_009567675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	neprilysin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009246299.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAGT CAGAAAGTCA GATGGATATA ACTGAAATGA ACACTCCAAA ACCAAAGAAA 
AAACTGCGAT GGAGTGCCCT GGAGATAGGG CTGGCTGTTG TGGTGGTCCT GCTGGCCATT 
GCCACCATCA CCATGAGTGT TCTGTATGCA ACATATGATG ATGGGGTTTG CAAGACATCA 
GACTGTATAA AATCAGCAGC CCGAATCCTT GAGAACATGG ACACCACCGC AGAGCCTTGT 
AATGATTTTT ACCAATATGC CTGTGGAGGA TGGCTTAAGA GGAACGTCAT TCCAGAAACC 
AGCTCCCGTT ATAGTAACTT TGACATTTTA AGAGACGAAC TAGAAGTTGT TTTAAAAGAT 
GTCCTCGACA CACCGAGCAC TAATGACATA ACAGCAGTGC AGAAGGCAAA AACTCTGTAC 
AGATCATGCA TAAATGAAAC TGCCATCGAC AGCAGAGGTG GAATGCCTTT AATCAGTTTA 
TTGCCAAATG TATCTGACTG GCCTGTAGCA ACAAGTAACT GGGAAGCTTC CTATGGTACT 
GCTTGGACAG CTGAGAGAGC TATTGCACAG CTGAACTCCA GATATGGGAA AAAAGTCCTT 
ATTAATTTTT TTGTTGGCAC GGACGATAAA AATTCCTCAG CATACATCAT TCATATTGAT 
CAGCCCGGGC TTGGCCTGCC TTCTCGTGAC TACTACGAGT GCACTGGTGC ATACAGAGAG 
GCGTGTTCTG CCTATGTTGA TTTCATGATT TCTGTAGCTA AACTAATCCT ACAAGAAAGA 
AACATTTCTG TCAATGAGAA TGAGATTTCT GAACAAATGG GAAGAGTTAT GGAGCTGGAG 
AAAGAGATTG CCAATGCAAC GACAAAATCT GAAGACAGAA ATGATCCCCT TTTGCAATAC 
AATAAAATGA CCCTGGCACA ACTCCAAAGC AACTTCTCAT TGGAAATTGA TAAAAAGGTA 
TTCGACTGGT CAAAATTCAT AAATGATATA ATGTCAACTG TACAAATTAA CATTGAGAAC 
ACTGAATATG TCATTGTTTA TGATCCAGAC TACCTTATCA AGCTGAAGTC TATTCTTAAT 
AAATACAGTC CCAGAGACCT TCAAAATTAC ATGGTCTGGC GATTCATAAT GGATCTTGTC 
AACAGTCTAA GCCGTAATTA CAAGGACACA AGGAATGCGT TTCGCAAGGC ACTTTATGGC 
ACGACATCAG AAACTGCTGT GTGGCGGCGT TGCGCAAACT ATGTCAATGG CAACATGGAG 
AACGCGGTGG GACGATTATA TGTGAAGGAA GCATTTGCAG GAGACAATAA ACATGTGGTC 
GAGGAAATGA TTGCAGATAT ACGCGATGTT TTTATAAAAA CCTTAGATGA GCTTACCTGG 
ATGGATGCAG AAACCAAAAA GAAAGCAGAA CAAAAAGCCA CAGCCATTAG AGAGAGGATC 
GGTTATCCGG ATGAGATCGT GACTGATGAC AATAAGCTGA ACAGTGAGTA CCAGGAGCTG 
AACTACAAAG AAGAAGAATA CTTTGAAAAC ATGATTCAAA ATTTAGTATT TACCCAGAAG 
AAGACGTTGA AAAAGCTTAG AGAAAAGGTG GACAAAGAGG AGTGGATAAG TGGAGCTGCT 
GTGGTCAATG CTTTTTATTC ATCGAGCAGA AATCAGATAG TCTTCCCTGC TGGCATTTTG 
CAGCCCCCTT TCTTCAGTTC ATCCCAGCCC AAATCTCTGA ATTATGGTGG CATTGGCATG 
GTCATCGGTC ATGAAATCAC ACATGGTTTT GATGACAATG GCAGAAATTT TAATGAGAAT 
GGAGACCTTG TCGACTGGTG GACTGAAGAA TCAGCACGCA AATTTAAGGA GCTATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTTCC TGGGATCTAG CAGGTGGACA GCACCTGAGT 
GGAATCAACA CACTAGGAGA AAACATTGCT GATAATGGGG GTGTTAGACA AGCATATAAG 
GCCTATGAAA ATTTTGTGAA AAAGCATGGA ACAGAAAAAC TTCTTCCTGG TCTTGAAATG 
AACCATAAGC AGCTGTTTTT TCTGAACTTC GCACAGGTAT GGTGTGGAAC ATACAGACCA 
GAATATGCTG TGAACTCCAT TAAAACTGAC TTGCACAGCC CAGGCAAATT CAGGGTCATA 
GGATCATTGC AAAACTCTGT GGAGTTTGCT GAAGCGTTTT CATGCACCAA GACAAACTAC 
ATGGATCCTG CGAAAAAGTG CCGAGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009565970.1
CDS	2..2254
Translation	MGKSESQMDITEMNTPKPKKKLRWSALEIGLAVVVVLLAIATITMSVLYATYDDGVCKTSDCIKSAARILENMDTTAEPCNDFYQYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLDTPSTNDITAVQKAKTLYRSCINETAIDSRGGMPLISLLPNVSDWPVATSNWEASYGTAWTAERAIAQLNSRYGKKVLINFFVGTDDKNSSAYIIHIDQPGLGLPSRDYYECTGAYREACSAYVDFMISVAKLILQERNISVNENEISEQMGRVMELEKEIANATTKSEDRNDPLLQYNKMTLAQLQSNFSLEIDKKVFDWSKFINDIMSTVQINIENTEYVIVYDPDYLIKLKSILNKYSPRDLQNYMVWRFIMDLVNSLSRNYKDTRNAFRKALYGTTSETAVWRRCANYVNGNMENAVGRLYVKEAFAGDNKHVVEEMIADIRDVFIKTLDELTWMDAETKKKAEQKATAIRERIGYPDEIVTDDNKLNSEYQELNYKEEEYFENMIQNLVFTQKKTLKKLREKVDKEEWISGAAVVNAFYSSSRNQIVFPAGILQPPFFSSSQPKSLNYGGIGMVIGHEITHGFDDNGRNFNENGDLVDWWTEESARKFKELSQCMVYQYGNFSWDLAGGQHLSGINTLGENIADNGGVRQAYKAYENFVKKHGTEKLLPGLEMNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDLHSPGKFRVIGSLQNSVEFAEAFSCTKTNYMDPAKKCRVW

Target ORF information:

RefSeq Version	XM_009567675.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus membrane metallo-endopeptidase (MME), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009567675.1

ORF Insert Sequence:

ATGGGCAAGT CAGAAAGTCA GATGGATATA ACTGAAATGA ACACTCCAAA ACCAAAGAAA 
AAACTGCGAT GGAGTGCCCT GGAGATAGGG CTGGCTGTTG TGGTGGTCCT GCTGGCCATT 
GCCACCATCA CCATGAGTGT TCTGTATGCA ACATATGATG ATGGGGTTTG CAAGACATCA 
GACTGTATAA AATCAGCAGC CCGAATCCTT GAGAACATGG ACACCACCGC AGAGCCTTGT 
AATGATTTTT ACCAATATGC CTGTGGAGGA TGGCTTAAGA GGAACGTCAT TCCAGAAACC 
AGCTCCCGTT ATAGTAACTT TGACATTTTA AGAGACGAAC TAGAAGTTGT TTTAAAAGAT 
GTCCTCGACA CACCGAGCAC TAATGACATA ACAGCAGTGC AGAAGGCAAA AACTCTGTAC 
AGATCATGCA TAAATGAAAC TGCCATCGAC AGCAGAGGTG GAATGCCTTT AATCAGTTTA 
TTGCCAAATG TATCTGACTG GCCTGTAGCA ACAAGTAACT GGGAAGCTTC CTATGGTACT 
GCTTGGACAG CTGAGAGAGC TATTGCACAG CTGAACTCCA GATATGGGAA AAAAGTCCTT 
ATTAATTTTT TTGTTGGCAC GGACGATAAA AATTCCTCAG CATACATCAT TCATATTGAT 
CAGCCCGGGC TTGGCCTGCC TTCTCGTGAC TACTACGAGT GCACTGGTGC ATACAGAGAG 
GCGTGTTCTG CCTATGTTGA TTTCATGATT TCTGTAGCTA AACTAATCCT ACAAGAAAGA 
AACATTTCTG TCAATGAGAA TGAGATTTCT GAACAAATGG GAAGAGTTAT GGAGCTGGAG 
AAAGAGATTG CCAATGCAAC GACAAAATCT GAAGACAGAA ATGATCCCCT TTTGCAATAC 
AATAAAATGA CCCTGGCACA ACTCCAAAGC AACTTCTCAT TGGAAATTGA TAAAAAGGTA 
TTCGACTGGT CAAAATTCAT AAATGATATA ATGTCAACTG TACAAATTAA CATTGAGAAC 
ACTGAATATG TCATTGTTTA TGATCCAGAC TACCTTATCA AGCTGAAGTC TATTCTTAAT 
AAATACAGTC CCAGAGACCT TCAAAATTAC ATGGTCTGGC GATTCATAAT GGATCTTGTC 
AACAGTCTAA GCCGTAATTA CAAGGACACA AGGAATGCGT TTCGCAAGGC ACTTTATGGC 
ACGACATCAG AAACTGCTGT GTGGCGGCGT TGCGCAAACT ATGTCAATGG CAACATGGAG 
AACGCGGTGG GACGATTATA TGTGAAGGAA GCATTTGCAG GAGACAATAA ACATGTGGTC 
GAGGAAATGA TTGCAGATAT ACGCGATGTT TTTATAAAAA CCTTAGATGA GCTTACCTGG 
ATGGATGCAG AAACCAAAAA GAAAGCAGAA CAAAAAGCCA CAGCCATTAG AGAGAGGATC 
GGTTATCCGG ATGAGATCGT GACTGATGAC AATAAGCTGA ACAGTGAGTA CCAGGAGCTG 
AACTACAAAG AAGAAGAATA CTTTGAAAAC ATGATTCAAA ATTTAGTATT TACCCAGAAG 
AAGACGTTGA AAAAGCTTAG AGAAAAGGTG GACAAAGAGG AGTGGATAAG TGGAGCTGCT 
GTGGTCAATG CTTTTTATTC ATCGAGCAGA AATCAGATAG TCTTCCCTGC TGGCATTTTG 
CAGCCCCCTT TCTTCAGTTC ATCCCAGCCC AAATCTCTGA ATTATGGTGG CATTGGCATG 
GTCATCGGTC ATGAAATCAC ACATGGTTTT GATGACAATG GCAGAAATTT TAATGAGAAT 
GGAGACCTTG TCGACTGGTG GACTGAAGAA TCAGCACGCA AATTTAAGGA GCTATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTTCC TGGGATCTAG CAGGTGGACA GCACCTGAGT 
GGAATCAACA CACTAGGAGA AAACATTGCT GATAATGGGG GTGTTAGACA AGCATATAAG 
GCCTATGAAA ATTTTGTGAA AAAGCATGGA ACAGAAAAAC TTCTTCCTGG TCTTGAAATG 
AACCATAAGC AGCTGTTTTT TCTGAACTTC GCACAGGTAT GGTGTGGAAC ATACAGACCA 
GAATATGCTG TGAACTCCAT TAAAACTGAC TTGCACAGCC CAGGCAAATT CAGGGTCATA 
GGATCATTGC AAAACTCTGT GGAGTTTGCT GAAGCGTTTT CATGCACCAA GACAAACTAC 
ATGGATCCTG CGAAAAAGTG CCGAGTTTGG TGA