LOC105323589 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105323589
Entrez Gene ID	105323589
Full Name	cytoplasmic tRNA 2-thiolation protein 1
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011422646.2	XP_011420948.1	cytoplasmic tRNA 2-thiolation protein 1

Pathways from BioSystems
Gene Ontology

The following LOC105323589 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105323589 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr123953	XM_011422646.2 Latest version!	Crassostrea gigas cytoplasmic tRNA 2-thiolation protein 1 (LOC105323589), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105323589 ( XM_011422646.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011420948.1 Crassostrea gigas cytoplasmic tRNA 2-thiolation protein 1 (LOC105323589), mRNA.

Price & Availability↑

CloneID	OCr123953
Clone ID Related Accession (Same CDS sequence)	XM_011422646.2
Accession Version	XM_011422646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	cytoplasmic tRNA 2-thiolation protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011940545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011422646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATCA AGTGTAACTT GTGTGAAGCG GGAGCTATTT TAAAACGACC TAAAACGGGA 
GATGCTCTGT GTAAGAACTG TTTCTACTTT GCCTTTGAAG AGGAAGTCCA CAAAACCATC 
ACAGATGCCA AACTGTTTGT CCGAGGGGAG ACGGTTGCCA TAGGAGCCTC AGGGGGCAAA 
GATTCCACAG TGCTGGCATA TGTGCTGAAA GCCCTTAACC AGAGATACGA CTACGGCGTG 
AATCTTGTCC TGTTGTCTGT GGACGAGGGG ATCACGGGGT ACAGGGACGA CTCACTGGAG 
ACGGTGAAGA GGAACCAGCA GCAGTATGAC CTGCCCCTGA AGATTGTGTC GTACGAGGAG 
CTGTATGGCT GGACCATGGA CGCCATTGTT AAACAGATCG GCCTCAAAAA TAACTGTACA 
TTCTGTGGAG TGTTTAGACG CCAGGCTTTG GATAGAGGAG CTATCCTGAT GAAAGTGGAT 
AGGATAGTTA CAGGTCACAA TGCAGATGAC ATTGCAGAAA CAGTGATAAT GAACGTGCTT 
CGAGGAGACA TAGGAAGATT GCAGAGATGC ACAGCCATCA CCACGGGGAC AGAGGGGGCC 
CTGCCCAGAA GTAAACCATT CAAGTACACA TACGAAAAGG AGATTGTCAT GTATGCTTAT 
TTCAAGAAGT TGGACTATTT TTCCACTGAA TGTATATATT CCCCAAATGC ATACAGAGGG 
TTTGCTAGAG CCTACATCAA AGATTTAGAG AAGATTAGAC CTAGTGCAAT TATTGACATA 
ATCCACTCGG GAGAGAGTCT GTCTGTGAAG AAAGCAGTGA AACTTCCCAC ACAAGGAACT 
TGTGAGCGGT GTGGCTACAT CTCCAGTAAC CCTCTATGTA AAGCCTGTAT CTTGTTGGAG 
GGGCTCAATA GGGGGCTACC CAGGCTGGGA ATCGGGAAAA GTCACAAAGA AAGAAGGAAG 
CTGAATGAAA TGTTGGCACA GAAAGAAGCG ACTGGTAATG AGAAGACAAC TCCTGACAAC 
TCAAGGGAGA CAACTGAGAA TGCTTCCTCT GAGAAGTCAG CATCAAGGAG CTCAAAACAG 
AAGTGTGAGA ACTGTAGCTG TGGACAGAAA TGTCTGTCAG AGAAAGTGGA GGACTTACAG 
CTGGACAGAA GGAAAATAGA CCCTAACCTC ATAGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011420948.1
CDS	111..1292
Translation	MPIKCNLCEAGAILKRPKTGDALCKNCFYFAFEEEVHKTITDAKLFVRGETVAIGASGGKDSTVLAYVLKALNQRYDYGVNLVLLSVDEGITGYRDDSLETVKRNQQQYDLPLKIVSYEELYGWTMDAIVKQIGLKNNCTFCGVFRRQALDRGAILMKVDRIVTGHNADDIAETVIMNVLRGDIGRLQRCTAITTGTEGALPRSKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFARAYIKDLEKIRPSAIIDIIHSGESLSVKKAVKLPTQGTCERCGYISSNPLCKACILLEGLNRGLPRLGIGKSHKERRKLNEMLAQKEATGNEKTTPDNSRETTENASSEKSASRSSKQKCENCSCGQKCLSEKVEDLQLDRRKIDPNLIDF

Target ORF information:

RefSeq Version	XM_011422646.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas cytoplasmic tRNA 2-thiolation protein 1 (LOC105323589), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011422646.2

ORF Insert Sequence:

ATGCCAATCA AGTGTAACTT GTGTGAAGCG GGAGCTATTT TAAAACGACC TAAAACGGGA 
GATGCTCTGT GTAAGAACTG TTTCTACTTT GCCTTTGAAG AGGAAGTCCA CAAAACCATC 
ACAGATGCCA AACTGTTTGT CCGAGGGGAG ACGGTTGCCA TAGGAGCCTC AGGGGGCAAA 
GATTCCACAG TGCTGGCATA TGTGCTGAAA GCCCTTAACC AGAGATACGA CTACGGCGTG 
AATCTTGTCC TGTTGTCTGT GGACGAGGGG ATCACGGGGT ACAGGGACGA CTCACTGGAG 
ACGGTGAAGA GGAACCAGCA GCAGTATGAC CTGCCCCTGA AGATTGTGTC GTACGAGGAG 
CTGTATGGCT GGACCATGGA CGCCATTGTT AAACAGATCG GCCTCAAAAA TAACTGTACA 
TTCTGTGGAG TGTTTAGACG CCAGGCTTTG GATAGAGGAG CTATCCTGAT GAAAGTGGAT 
AGGATAGTTA CAGGTCACAA TGCAGATGAC ATTGCAGAAA CAGTGATAAT GAACGTGCTT 
CGAGGAGACA TAGGAAGATT GCAGAGATGC ACAGCCATCA CCACGGGGAC AGAGGGGGCC 
CTGCCCAGAA GTAAACCATT CAAGTACACA TACGAAAAGG AGATTGTCAT GTATGCTTAT 
TTCAAGAAGT TGGACTATTT TTCCACTGAA TGTATATATT CCCCAAATGC ATACAGAGGG 
TTTGCTAGAG CCTACATCAA AGATTTAGAG AAGATTAGAC CTAGTGCAAT TATTGACATA 
ATCCACTCGG GAGAGAGTCT GTCTGTGAAG AAAGCAGTGA AACTTCCCAC ACAAGGAACT 
TGTGAGCGGT GTGGCTACAT CTCCAGTAAC CCTCTATGTA AAGCCTGTAT CTTGTTGGAG 
GGGCTCAATA GGGGGCTACC CAGGCTGGGA ATCGGGAAAA GTCACAAAGA AAGAAGGAAG 
CTGAATGAAA TGTTGGCACA GAAAGAAGCG ACTGGTAATG AGAAGACAAC TCCTGACAAC 
TCAAGGGAGA CAACTGAGAA TGCTTCCTCT GAGAAGTCAG CATCAAGGAG CTCAAAACAG 
AAGTGTGAGA ACTGTAGCTG TGGACAGAAA TGTCTGTCAG AGAAAGTGGA GGACTTACAG 
CTGGACAGAA GGAAAATAGA CCCTAACCTC ATAGACTTCT GA