CRIM1 cDNA ORF clone, Coturnix japonica(Japanese quail)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	107311287
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Coturnix japonica(Japanese quail)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015857694.1	XP_015713180.1	cysteine-rich motor neuron 1 protein isoform X2
XM_015857693.1	XP_015713179.1	cysteine-rich motor neuron 1 protein isoform X1

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo28179	XM_015857693.1 Latest version!	Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCo28180	XM_015857694.1 Latest version!	Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_015857693.1 ) cDNA ORF clone, Coturnix japonica(Japanese quail) -> XP_015713179.1 Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo28179
Clone ID Related Accession (Same CDS sequence)	XM_015857693.1
Accession Version	XM_015857693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-08
Organism	Coturnix japonica(Japanese quail)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015438621.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Coturnix japonica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG GGCGGAGGAC GCACTCGGAG CGGGGCGAGC CGCAGGAGGA GGCGAGTCGG 
GACTCGGGCT GCGAGCGGGG CACAGGCGCC GCCGCACCCA GGGGCTCGCA GCCGCTCGCG 
CGCGATCGCC GCTCCTCCGG CGTGGACGGG GCCGGAGGGC GAGGAACGCG CGAACCGACG 
GACGCCGCTT CCCGCGAAGG GACAGCGGGC GGAGCCGCCG CCGCCCGGAG CAGAAGTTTG 
GCTGGAGCGG CCGCCGCCGA GAGAGGAAAC TTTCCTCGGG GGGCCGGAGG CGGGGGCGGC 
GGGAAGGAGC CGCCGCTCGG CGGCTTTTCG CGGCGCGCTC GGGGTGCTCC GGCGCGGCTC 
CCCCCCGGCT CGGGCCGGGC GGCGGCGGCG GCCGGGATGT ACCTGGCAGC GGTCTCGGCG 
GGGAGGAGGC GGCCGGGCGG AGACGGCGGC GGAGGTGGCG GCGGCTGGCA CCTGGCGGCG 
GCGGGGTGGC TGCTGCTGCT GGCGTTGCTG CTCGGCCAGC CGGGGACGCG GGCGCTCGTC 
TGCCTGCCCT GCGACGAGTC CAAGTGCGAG GAGCCCAAGA GCTGCCCCGG CAGCGTCGTG 
CTGGGCATCT GCGGCTGCTG CTCCATGTGC GCCCGGCAGC GCAACGAGAG CTGCGGGGGC 
GTCTACGGGC TGCACGGCGC CTGCGACCGG GGGCTGCGCT GTGTCATCCG CCCGCCGCTC 
AACGGCGACT CCATCACCGA GTACGAAGTG GGCGTCTGTG AAGATGAAAA CTGGGATGAT 
GATCAGCTCC TTGGATTTGA GCCATGCAAT GAAAACCTTA TAACAGGTTG TAACATAATC 
AACGGGAAAT GTGAATGTGA CACTATTCGG ACCTGTAACA ATCCATTTGA ATTTCCAAGT 
CGGGATACTT GCCTGTCAGC CTTGAAAAGG ATTGAAGAAG AGAAACCTGA TTGCTCCAAG 
GCCCGCTGTG AGGTCCAGTT TTCTCCTCGC TGTCCAGAAG ATTCCATCTT GATTGAAGGT 
TATGCTCCTC CTGGAGAATG CTGTCCTCTC CCAAGCCGTT GTGTGTGTAA CCCTGCAGGC 
TGCTTGAGGA AGGTTTGCCA GCCTGGCTAT TTGAATATAT TGGTTTCTAA GGCATCAGGA 
AAGCCAGGGG AATGCTGTGA TCTCTATGAA TGTAAACCAG TGTTCAGTGT GGACTGCAGC 
ACAGTGGAAT GCCCTCCTGT TCAGCAAGTG GTTTGCCCAC TGGATAGCTA TGAAACCCAA 
GTCAGGCTGA CGGCTGATGG CTGCTGTACC CTACCCACAA GATGCGAGTG TCTCTCTGGT 
TTATGTGGTT TCCCCATGTG CGAGGCAGGC TCCGTTCCTC AAATAGTCTC ACGTGGAGAT 
GGAACACCTG GCAAGTGCTG TGATGTCTTT GAGTGTGTCA ATGAAGTGAA GCCAACTTGC 
ATATTTAACA GCGTGGAATA TTATGATGGA GACATGTTTC GAATGGATGC TTGTCGCTTC 
TGTCGGTGTC AAGGTGGTGT CTCTATTTGC TTCTCTGCCC AGTGTGGTGA GCTACACTGT 
GACAGTTATT ATGTGCCAGA GGGAGAGTGC TGCCCAGTGT GTGAAGACCC AGTTTACCCT 
GTAAATAATC CCGCTGGCTG TTATGCCAAT GGTCAGATCC AAGCTCATGG AGACCGCTGG 
AGAGAGGATG ACTGTACTTT CTGCCAGTGT ATCAACGGAG ATGCTCACTG TGTTGCTACA 
GCCTGTGGGC AGAGCTGTTT GAATCCTGTC AAAGTCCCAG GAGAGTGCTG CCCAGTATGT 
GAAGAGCCGA CTTACATAAC AATAGGTCCA CCCACCTGCG AGCTCTTGGT GAACTGCACT 
TTGACCGAGA AAGACTGTAT CTATAGTTTC AAACTGGATC AAAATGGTTG CCGCATTTGT 
CAGTGCAAAA CCAGAGAAGA GCTGTGTACA GGCCTCATAT CAGGCTGCTC CCTGGAATGT 
TCCTTTGGGT TCCAGACTGA TGCCCACAAC TGTGAGATCT GTCAGTGTCG TCCACGGCCC 
AAGAAATGCA AACCTATTGT ATGTGACAAG TATTGTCCCT TTGGATACGT GAAGAACAAG 
CATGGCTGTG AAATCTGTCG ATGTAAGAAA TGCCCAGAAA TGCCTTGTGG TAAAATCTGC 
CCAATGGGCT TCCAGCAGAA CAGCCACGGG TGTGTTATCT GCAAATGCAG AGAGGCAACT 
GCTTCTCTTA TGCCTCCAGT TAAAACAGGC TCTTGCCTGT CAATGGATGG GCGCCGACAT 
GAAAATGAGG AGAGTTGGCA CGATGGATGC AGGGAATGTT ACTGTCACAA TGGACGAGAA 
ATGTGTGCCT TGATCACCTG CCCTGTTCCT AATTGTGGCA ACCCCACCAT ACATCCGGGG 
CAGTGTTGCC CATCATGTCC AGATGAAATA GTTGTGCAGA AGCCAGAGCT CACTAGTCCA 
TCAATCTGTC ATGCTCCTGG TGGGGAATAC TTTGTAGAAG GAGAAACATG GAATATTGAT 
ACTTGCACAC AATGTACATG CCACAGCGGA CGAGTCCTGT GTGAGACTGA AGTCTGTCCT 
CCTCTTCTTT GTCAAAACCC CACTCGCACA CAGGACTCCT GCTGTCCTCA GTGCCCAGAT 
GAGCCTCTTC AGCCTTCGTC AAGCAATGTC AGCATGCCCA GTTACTGCAA AAATGATGAA 
GGAGATATTT TTCTGACAGC TGAGTCCTGG AAGCCCAACG TCTGCACCAG CTGCATCTGC 
ATGGATGGTG TAATTAGATG CTACTCTGAG TCTTGTCCAC CAGTATCCTG TGAGAGACCT 
GTTTTGAGAA AGGGACAGTG TTGTCCTTAC TGTATTGAAG ATACAGTACC AAAGAAAGTG 
GTCTGCCACT TCAATGGAAA AACATATGCA GATGAGGAAC GCTGGGACAT CGACAGCTGC 
ACGCACTGCT ACTGCCTGCA GGGCCAGACT CTTTGCTCCA CTGTCAGCTG CCCACCCCTC 
CCCTGTGCTG AACCCATCAA TGTGGAGGGA AGCTGCTGTC CCATGTGCCC AGAGATGTAT 
GTACCTGAAC CTACTAACAT CCCCATTGAG AAGACTAATC ATCGAGGAGA CGTTGATCTA 
GAAGTGCCAA ACTGGTCAAC GCCTAGTGAA AATGACATCA TCCACATTCA CAGAGACATG 
AATCATCTCC AGGGAGAGTA CAGAAGTGGA AATGGGCCAC ACCCCAGTGA AGATGCGTCA 
GTTAGCTCTG TTGCCTTGGT GACAATTCCA ATCACGATAG CGCTGTTAGT AATCATCATC 
TTTCTGCTCA TCAATCAGAA AAAGCAGTGG ATTCCAGTGT CCTGCTACAA AGCTCCAACA 
AAGCCATCTT GCCTAAACAA TCAGCTGGTG TATGTGGACT GCAAGAAAGG TACTATGGTC 
CAGGTGGACA GTTCTCAGAG GATGCTAAGA ATTGCAGACC CGGATTCAAG ATACAGCGGA 
TTTTACAGCA TGCAGAAACA GAACAACCTA CAGGCAGATA ATTTCTATCA AACAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015713179.1
CDS	33..3572
Translation	MGEGRRTHSERGEPQEEASRDSGCERGTGAAAPRGSQPLARDRRSSGVDGAGGRGTREPTDAASREGTAGGAAAARSRSLAGAAAAERGNFPRGAGGGGGGKEPPLGGFSRRARGAPARLPPGSGRAAAAAGMYLAAVSAGRRRPGGDGGGGGGGWHLAAAGWLLLLALLLGQPGTRALVCLPCDESKCEEPKSCPGSVVLGICGCCSMCARQRNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLITGCNIINGKCECDTIRTCNNPFEFPSRDTCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSILIEGYAPPGECCPLPSRCVCNPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPMCEAGSVPQIVSRGDGTPGKCCDVFECVNEVKPTCIFNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCDSYYVPEGECCPVCEDPVYPVNNPAGCYANGQIQAHGDRWREDDCTFCQCINGDAHCVATACGQSCLNPVKVPGECCPVCEEPTYITIGPPTCELLVNCTLTEKDCIYSFKLDQNGCRICQCKTREELCTGLISGCSLECSFGFQTDAHNCEICQCRPRPKKCKPIVCDKYCPFGYVKNKHGCEICRCKKCPEMPCGKICPMGFQQNSHGCVICKCREATASLMPPVKTGSCLSMDGRRHENEESWHDGCRECYCHNGREMCALITCPVPNCGNPTIHPGQCCPSCPDEIVVQKPELTSPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSNVSMPSYCKNDEGDIFLTAESWKPNVCTSCICMDGVIRCYSESCPPVSCERPVLRKGQCCPYCIEDTVPKKVVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCAEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDVDLEVPNWSTPSENDIIHIHRDMNHLQGEYRSGNGPHPSEDASVSSVALVTIPITIALLVIIIFLLINQKKQWIPVSCYKAPTKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_015857693.1
Organism	Coturnix japonica(Japanese quail)
Definition	Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015857693.1

ORF Insert Sequence:

ATGGGGGAAG GGCGGAGGAC GCACTCGGAG CGGGGCGAGC CGCAGGAGGA GGCGAGTCGG 
GACTCGGGCT GCGAGCGGGG CACAGGCGCC GCCGCACCCA GGGGCTCGCA GCCGCTCGCG 
CGCGATCGCC GCTCCTCCGG CGTGGACGGG GCCGGAGGGC GAGGAACGCG CGAACCGACG 
GACGCCGCTT CCCGCGAAGG GACAGCGGGC GGAGCCGCCG CCGCCCGGAG CAGAAGTTTG 
GCTGGAGCGG CCGCCGCCGA GAGAGGAAAC TTTCCTCGGG GGGCCGGAGG CGGGGGCGGC 
GGGAAGGAGC CGCCGCTCGG CGGCTTTTCG CGGCGCGCTC GGGGTGCTCC GGCGCGGCTC 
CCCCCCGGCT CGGGCCGGGC GGCGGCGGCG GCCGGGATGT ACCTGGCAGC GGTCTCGGCG 
GGGAGGAGGC GGCCGGGCGG AGACGGCGGC GGAGGTGGCG GCGGCTGGCA CCTGGCGGCG 
GCGGGGTGGC TGCTGCTGCT GGCGTTGCTG CTCGGCCAGC CGGGGACGCG GGCGCTCGTC 
TGCCTGCCCT GCGACGAGTC CAAGTGCGAG GAGCCCAAGA GCTGCCCCGG CAGCGTCGTG 
CTGGGCATCT GCGGCTGCTG CTCCATGTGC GCCCGGCAGC GCAACGAGAG CTGCGGGGGC 
GTCTACGGGC TGCACGGCGC CTGCGACCGG GGGCTGCGCT GTGTCATCCG CCCGCCGCTC 
AACGGCGACT CCATCACCGA GTACGAAGTG GGCGTCTGTG AAGATGAAAA CTGGGATGAT 
GATCAGCTCC TTGGATTTGA GCCATGCAAT GAAAACCTTA TAACAGGTTG TAACATAATC 
AACGGGAAAT GTGAATGTGA CACTATTCGG ACCTGTAACA ATCCATTTGA ATTTCCAAGT 
CGGGATACTT GCCTGTCAGC CTTGAAAAGG ATTGAAGAAG AGAAACCTGA TTGCTCCAAG 
GCCCGCTGTG AGGTCCAGTT TTCTCCTCGC TGTCCAGAAG ATTCCATCTT GATTGAAGGT 
TATGCTCCTC CTGGAGAATG CTGTCCTCTC CCAAGCCGTT GTGTGTGTAA CCCTGCAGGC 
TGCTTGAGGA AGGTTTGCCA GCCTGGCTAT TTGAATATAT TGGTTTCTAA GGCATCAGGA 
AAGCCAGGGG AATGCTGTGA TCTCTATGAA TGTAAACCAG TGTTCAGTGT GGACTGCAGC 
ACAGTGGAAT GCCCTCCTGT TCAGCAAGTG GTTTGCCCAC TGGATAGCTA TGAAACCCAA 
GTCAGGCTGA CGGCTGATGG CTGCTGTACC CTACCCACAA GATGCGAGTG TCTCTCTGGT 
TTATGTGGTT TCCCCATGTG CGAGGCAGGC TCCGTTCCTC AAATAGTCTC ACGTGGAGAT 
GGAACACCTG GCAAGTGCTG TGATGTCTTT GAGTGTGTCA ATGAAGTGAA GCCAACTTGC 
ATATTTAACA GCGTGGAATA TTATGATGGA GACATGTTTC GAATGGATGC TTGTCGCTTC 
TGTCGGTGTC AAGGTGGTGT CTCTATTTGC TTCTCTGCCC AGTGTGGTGA GCTACACTGT 
GACAGTTATT ATGTGCCAGA GGGAGAGTGC TGCCCAGTGT GTGAAGACCC AGTTTACCCT 
GTAAATAATC CCGCTGGCTG TTATGCCAAT GGTCAGATCC AAGCTCATGG AGACCGCTGG 
AGAGAGGATG ACTGTACTTT CTGCCAGTGT ATCAACGGAG ATGCTCACTG TGTTGCTACA 
GCCTGTGGGC AGAGCTGTTT GAATCCTGTC AAAGTCCCAG GAGAGTGCTG CCCAGTATGT 
GAAGAGCCGA CTTACATAAC AATAGGTCCA CCCACCTGCG AGCTCTTGGT GAACTGCACT 
TTGACCGAGA AAGACTGTAT CTATAGTTTC AAACTGGATC AAAATGGTTG CCGCATTTGT 
CAGTGCAAAA CCAGAGAAGA GCTGTGTACA GGCCTCATAT CAGGCTGCTC CCTGGAATGT 
TCCTTTGGGT TCCAGACTGA TGCCCACAAC TGTGAGATCT GTCAGTGTCG TCCACGGCCC 
AAGAAATGCA AACCTATTGT ATGTGACAAG TATTGTCCCT TTGGATACGT GAAGAACAAG 
CATGGCTGTG AAATCTGTCG ATGTAAGAAA TGCCCAGAAA TGCCTTGTGG TAAAATCTGC 
CCAATGGGCT TCCAGCAGAA CAGCCACGGG TGTGTTATCT GCAAATGCAG AGAGGCAACT 
GCTTCTCTTA TGCCTCCAGT TAAAACAGGC TCTTGCCTGT CAATGGATGG GCGCCGACAT 
GAAAATGAGG AGAGTTGGCA CGATGGATGC AGGGAATGTT ACTGTCACAA TGGACGAGAA 
ATGTGTGCCT TGATCACCTG CCCTGTTCCT AATTGTGGCA ACCCCACCAT ACATCCGGGG 
CAGTGTTGCC CATCATGTCC AGATGAAATA GTTGTGCAGA AGCCAGAGCT CACTAGTCCA 
TCAATCTGTC ATGCTCCTGG TGGGGAATAC TTTGTAGAAG GAGAAACATG GAATATTGAT 
ACTTGCACAC AATGTACATG CCACAGCGGA CGAGTCCTGT GTGAGACTGA AGTCTGTCCT 
CCTCTTCTTT GTCAAAACCC CACTCGCACA CAGGACTCCT GCTGTCCTCA GTGCCCAGAT 
GAGCCTCTTC AGCCTTCGTC AAGCAATGTC AGCATGCCCA GTTACTGCAA AAATGATGAA 
GGAGATATTT TTCTGACAGC TGAGTCCTGG AAGCCCAACG TCTGCACCAG CTGCATCTGC 
ATGGATGGTG TAATTAGATG CTACTCTGAG TCTTGTCCAC CAGTATCCTG TGAGAGACCT 
GTTTTGAGAA AGGGACAGTG TTGTCCTTAC TGTATTGAAG ATACAGTACC AAAGAAAGTG 
GTCTGCCACT TCAATGGAAA AACATATGCA GATGAGGAAC GCTGGGACAT CGACAGCTGC 
ACGCACTGCT ACTGCCTGCA GGGCCAGACT CTTTGCTCCA CTGTCAGCTG CCCACCCCTC 
CCCTGTGCTG AACCCATCAA TGTGGAGGGA AGCTGCTGTC CCATGTGCCC AGAGATGTAT 
GTACCTGAAC CTACTAACAT CCCCATTGAG AAGACTAATC ATCGAGGAGA CGTTGATCTA 
GAAGTGCCAA ACTGGTCAAC GCCTAGTGAA AATGACATCA TCCACATTCA CAGAGACATG 
AATCATCTCC AGGGAGAGTA CAGAAGTGGA AATGGGCCAC ACCCCAGTGA AGATGCGTCA 
GTTAGCTCTG TTGCCTTGGT GACAATTCCA ATCACGATAG CGCTGTTAGT AATCATCATC 
TTTCTGCTCA TCAATCAGAA AAAGCAGTGG ATTCCAGTGT CCTGCTACAA AGCTCCAACA 
AAGCCATCTT GCCTAAACAA TCAGCTGGTG TATGTGGACT GCAAGAAAGG TACTATGGTC 
CAGGTGGACA GTTCTCAGAG GATGCTAAGA ATTGCAGACC CGGATTCAAG ATACAGCGGA 
TTTTACAGCA TGCAGAAACA GAACAACCTA CAGGCAGATA ATTTCTATCA AACAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_015857694.1 ) cDNA ORF clone, Coturnix japonica(Japanese quail) -> XP_015713180.1 Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo28180
Clone ID Related Accession (Same CDS sequence)	XM_015857694.1
Accession Version	XM_015857694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-08
Organism	Coturnix japonica(Japanese quail)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015438621.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Coturnix japonica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG GGCGGAGGAC GCACTCGGAG CGGGGCGAGC CGCAGGAGGA GGCGAGTCGG 
GACTCGGGCT GCGAGCGGGG CACAGGCGCC GCCGCACCCA GGGGCTCGCA GCCGCTCGCG 
CGCGATCGCC GCTCCTCCGG CGTGGACGGG GCCGGAGGGC GAGGAACGCG CGAACCGACG 
GACGCCGCTT CCCGCGAAGG GACAGCGGGC GGAGCCGCCG CCGCCCGGAG CAGAAGTTTG 
GCTGGAGCGG CCGCCGCCGA GAGAGGAAAC TTTCCTCGGG GGGCCGGAGG CGGGGGCGGC 
GGGAAGGAGC CGCCGCTCGG CGGCTTTTCG CGGCGCGCTC GGGGTGCTCC GGCGCGGCTC 
CCCCCCGGCT CGGGCCGGGC GGCGGCGGCG GCCGGGATGT ACCTGGCAGC GGTCTCGGCG 
GGGAGGAGGC GGCCGGGCGG AGACGGCGGC GGAGGTGGCG GCGGCTGGCA CCTGGCGGCG 
GCGGGGTGGC TGCTGCTGCT GGCGTTGCTG CTCGGCCAGC CGGGGACGCG GGCGCTCGTC 
TGCCTGCCCT GCGACGAGTC CAAGTGCGAG GAGCCCAAGA GCTGCCCCGG CAGCGTCGTG 
CTGGGCATCT GCGGCTGCTG CTCCATGTGC GCCCGGCAGC GCAACGAGAG CTGCGGGGGC 
GTCTACGGGC TGCACGGCGC CTGCGACCGG GGGCTGCGCT GTGTCATCCG CCCGCCGCTC 
AACGGCGACT CCATCACCGA GTACGAAGTG GGCGTCTGTG AAGATGAAAA CTGGGATGAT 
GATCAGCTCC TTGGATTTGA GCCATGCAAT GAAAACCTTA TAACAGGTTG TAACATAATC 
AACGGGAAAT GTGAATGTGA CACTATTCGG ACCTGTAACA ATCCATTTGA ATTTCCAAGT 
CGGGATACTT GCCTGTCAGC CTTGAAAAGG ATTGAAGTGT TCAGTGTGGA CTGCAGCACA 
GTGGAATGCC CTCCTGTTCA GCAAGTGGTT TGCCCACTGG ATAGCTATGA AACCCAAGTC 
AGGCTGACGG CTGATGGCTG CTGTACCCTA CCCACAAGAT GCGAGTGTCT CTCTGGTTTA 
TGTGGTTTCC CCATGTGCGA GGCAGGCTCC GTTCCTCAAA TAGTCTCACG TGGAGATGGA 
ACACCTGGCA AGTGCTGTGA TGTCTTTGAG TGTGTCAATG AAGTGAAGCC AACTTGCATA 
TTTAACAGCG TGGAATATTA TGATGGAGAC ATGTTTCGAA TGGATGCTTG TCGCTTCTGT 
CGGTGTCAAG GTGGTGTCTC TATTTGCTTC TCTGCCCAGT GTGGTGAGCT ACACTGTGAC 
AGTTATTATG TGCCAGAGGG AGAGTGCTGC CCAGTGTGTG AAGACCCAGT TTACCCTGTA 
AATAATCCCG CTGGCTGTTA TGCCAATGGT CAGATCCAAG CTCATGGAGA CCGCTGGAGA 
GAGGATGACT GTACTTTCTG CCAGTGTATC AACGGAGATG CTCACTGTGT TGCTACAGCC 
TGTGGGCAGA GCTGTTTGAA TCCTGTCAAA GTCCCAGGAG AGTGCTGCCC AGTATGTGAA 
GAGCCGACTT ACATAACAAT AGGTCCACCC ACCTGCGAGC TCTTGGTGAA CTGCACTTTG 
ACCGAGAAAG ACTGTATCTA TAGTTTCAAA CTGGATCAAA ATGGTTGCCG CATTTGTCAG 
TGCAAAACCA GAGAAGAGCT GTGTACAGGC CTCATATCAG GCTGCTCCCT GGAATGTTCC 
TTTGGGTTCC AGACTGATGC CCACAACTGT GAGATCTGTC AGTGTCGTCC ACGGCCCAAG 
AAATGCAAAC CTATTGTATG TGACAAGTAT TGTCCCTTTG GATACGTGAA GAACAAGCAT 
GGCTGTGAAA TCTGTCGATG TAAGAAATGC CCAGAAATGC CTTGTGGTAA AATCTGCCCA 
ATGGGCTTCC AGCAGAACAG CCACGGGTGT GTTATCTGCA AATGCAGAGA GGCAACTGCT 
TCTCTTATGC CTCCAGTTAA AACAGGCTCT TGCCTGTCAA TGGATGGGCG CCGACATGAA 
AATGAGGAGA GTTGGCACGA TGGATGCAGG GAATGTTACT GTCACAATGG ACGAGAAATG 
TGTGCCTTGA TCACCTGCCC TGTTCCTAAT TGTGGCAACC CCACCATACA TCCGGGGCAG 
TGTTGCCCAT CATGTCCAGA TGAAATAGTT GTGCAGAAGC CAGAGCTCAC TAGTCCATCA 
ATCTGTCATG CTCCTGGTGG GGAATACTTT GTAGAAGGAG AAACATGGAA TATTGATACT 
TGCACACAAT GTACATGCCA CAGCGGACGA GTCCTGTGTG AGACTGAAGT CTGTCCTCCT 
CTTCTTTGTC AAAACCCCAC TCGCACACAG GACTCCTGCT GTCCTCAGTG CCCAGATGAG 
CCTCTTCAGC CTTCGTCAAG CAATGTCAGC ATGCCCAGTT ACTGCAAAAA TGATGAAGGA 
GATATTTTTC TGACAGCTGA GTCCTGGAAG CCCAACGTCT GCACCAGCTG CATCTGCATG 
GATGGTGTAA TTAGATGCTA CTCTGAGTCT TGTCCACCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAGG GACAGTGTTG TCCTTACTGT ATTGAAGATA CAGTACCAAA GAAAGTGGTC 
TGCCACTTCA ATGGAAAAAC ATATGCAGAT GAGGAACGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACTCTT TGCTCCACTG TCAGCTGCCC ACCCCTCCCC 
TGTGCTGAAC CCATCAATGT GGAGGGAAGC TGCTGTCCCA TGTGCCCAGA GATGTATGTA 
CCTGAACCTA CTAACATCCC CATTGAGAAG ACTAATCATC GAGGAGACGT TGATCTAGAA 
GTGCCAAACT GGTCAACGCC TAGTGAAAAT GACATCATCC ACATTCACAG AGACATGAAT 
CATCTCCAGG GAGAGTACAG AAGTGGAAAT GGGCCACACC CCAGTGAAGA TGCGTCAGTT 
AGCTCTGTTG CCTTGGTGAC AATTCCAATC ACGATAGCGC TGTTAGTAAT CATCATCTTT 
CTGCTCATCA ATCAGAAAAA GCAGTGGATT CCAGTGTCCT GCTACAAAGC TCCAACAAAG 
CCATCTTGCC TAAACAATCA GCTGGTGTAT GTGGACTGCA AGAAAGGTAC TATGGTCCAG 
GTGGACAGTT CTCAGAGGAT GCTAAGAATT GCAGACCCGG ATTCAAGATA CAGCGGATTT 
TACAGCATGC AGAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015713180.1
CDS	33..3329
Translation	MGEGRRTHSERGEPQEEASRDSGCERGTGAAAPRGSQPLARDRRSSGVDGAGGRGTREPTDAASREGTAGGAAAARSRSLAGAAAAERGNFPRGAGGGGGGKEPPLGGFSRRARGAPARLPPGSGRAAAAAGMYLAAVSAGRRRPGGDGGGGGGGWHLAAAGWLLLLALLLGQPGTRALVCLPCDESKCEEPKSCPGSVVLGICGCCSMCARQRNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLITGCNIINGKCECDTIRTCNNPFEFPSRDTCLSALKRIEVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPMCEAGSVPQIVSRGDGTPGKCCDVFECVNEVKPTCIFNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCDSYYVPEGECCPVCEDPVYPVNNPAGCYANGQIQAHGDRWREDDCTFCQCINGDAHCVATACGQSCLNPVKVPGECCPVCEEPTYITIGPPTCELLVNCTLTEKDCIYSFKLDQNGCRICQCKTREELCTGLISGCSLECSFGFQTDAHNCEICQCRPRPKKCKPIVCDKYCPFGYVKNKHGCEICRCKKCPEMPCGKICPMGFQQNSHGCVICKCREATASLMPPVKTGSCLSMDGRRHENEESWHDGCRECYCHNGREMCALITCPVPNCGNPTIHPGQCCPSCPDEIVVQKPELTSPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSNVSMPSYCKNDEGDIFLTAESWKPNVCTSCICMDGVIRCYSESCPPVSCERPVLRKGQCCPYCIEDTVPKKVVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCAEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDVDLEVPNWSTPSENDIIHIHRDMNHLQGEYRSGNGPHPSEDASVSSVALVTIPITIALLVIIIFLLINQKKQWIPVSCYKAPTKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_015857694.1
Organism	Coturnix japonica(Japanese quail)
Definition	Coturnix japonica cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015857694.1

ORF Insert Sequence:

ATGGGGGAAG GGCGGAGGAC GCACTCGGAG CGGGGCGAGC CGCAGGAGGA GGCGAGTCGG 
GACTCGGGCT GCGAGCGGGG CACAGGCGCC GCCGCACCCA GGGGCTCGCA GCCGCTCGCG 
CGCGATCGCC GCTCCTCCGG CGTGGACGGG GCCGGAGGGC GAGGAACGCG CGAACCGACG 
GACGCCGCTT CCCGCGAAGG GACAGCGGGC GGAGCCGCCG CCGCCCGGAG CAGAAGTTTG 
GCTGGAGCGG CCGCCGCCGA GAGAGGAAAC TTTCCTCGGG GGGCCGGAGG CGGGGGCGGC 
GGGAAGGAGC CGCCGCTCGG CGGCTTTTCG CGGCGCGCTC GGGGTGCTCC GGCGCGGCTC 
CCCCCCGGCT CGGGCCGGGC GGCGGCGGCG GCCGGGATGT ACCTGGCAGC GGTCTCGGCG 
GGGAGGAGGC GGCCGGGCGG AGACGGCGGC GGAGGTGGCG GCGGCTGGCA CCTGGCGGCG 
GCGGGGTGGC TGCTGCTGCT GGCGTTGCTG CTCGGCCAGC CGGGGACGCG GGCGCTCGTC 
TGCCTGCCCT GCGACGAGTC CAAGTGCGAG GAGCCCAAGA GCTGCCCCGG CAGCGTCGTG 
CTGGGCATCT GCGGCTGCTG CTCCATGTGC GCCCGGCAGC GCAACGAGAG CTGCGGGGGC 
GTCTACGGGC TGCACGGCGC CTGCGACCGG GGGCTGCGCT GTGTCATCCG CCCGCCGCTC 
AACGGCGACT CCATCACCGA GTACGAAGTG GGCGTCTGTG AAGATGAAAA CTGGGATGAT 
GATCAGCTCC TTGGATTTGA GCCATGCAAT GAAAACCTTA TAACAGGTTG TAACATAATC 
AACGGGAAAT GTGAATGTGA CACTATTCGG ACCTGTAACA ATCCATTTGA ATTTCCAAGT 
CGGGATACTT GCCTGTCAGC CTTGAAAAGG ATTGAAGTGT TCAGTGTGGA CTGCAGCACA 
GTGGAATGCC CTCCTGTTCA GCAAGTGGTT TGCCCACTGG ATAGCTATGA AACCCAAGTC 
AGGCTGACGG CTGATGGCTG CTGTACCCTA CCCACAAGAT GCGAGTGTCT CTCTGGTTTA 
TGTGGTTTCC CCATGTGCGA GGCAGGCTCC GTTCCTCAAA TAGTCTCACG TGGAGATGGA 
ACACCTGGCA AGTGCTGTGA TGTCTTTGAG TGTGTCAATG AAGTGAAGCC AACTTGCATA 
TTTAACAGCG TGGAATATTA TGATGGAGAC ATGTTTCGAA TGGATGCTTG TCGCTTCTGT 
CGGTGTCAAG GTGGTGTCTC TATTTGCTTC TCTGCCCAGT GTGGTGAGCT ACACTGTGAC 
AGTTATTATG TGCCAGAGGG AGAGTGCTGC CCAGTGTGTG AAGACCCAGT TTACCCTGTA 
AATAATCCCG CTGGCTGTTA TGCCAATGGT CAGATCCAAG CTCATGGAGA CCGCTGGAGA 
GAGGATGACT GTACTTTCTG CCAGTGTATC AACGGAGATG CTCACTGTGT TGCTACAGCC 
TGTGGGCAGA GCTGTTTGAA TCCTGTCAAA GTCCCAGGAG AGTGCTGCCC AGTATGTGAA 
GAGCCGACTT ACATAACAAT AGGTCCACCC ACCTGCGAGC TCTTGGTGAA CTGCACTTTG 
ACCGAGAAAG ACTGTATCTA TAGTTTCAAA CTGGATCAAA ATGGTTGCCG CATTTGTCAG 
TGCAAAACCA GAGAAGAGCT GTGTACAGGC CTCATATCAG GCTGCTCCCT GGAATGTTCC 
TTTGGGTTCC AGACTGATGC CCACAACTGT GAGATCTGTC AGTGTCGTCC ACGGCCCAAG 
AAATGCAAAC CTATTGTATG TGACAAGTAT TGTCCCTTTG GATACGTGAA GAACAAGCAT 
GGCTGTGAAA TCTGTCGATG TAAGAAATGC CCAGAAATGC CTTGTGGTAA AATCTGCCCA 
ATGGGCTTCC AGCAGAACAG CCACGGGTGT GTTATCTGCA AATGCAGAGA GGCAACTGCT 
TCTCTTATGC CTCCAGTTAA AACAGGCTCT TGCCTGTCAA TGGATGGGCG CCGACATGAA 
AATGAGGAGA GTTGGCACGA TGGATGCAGG GAATGTTACT GTCACAATGG ACGAGAAATG 
TGTGCCTTGA TCACCTGCCC TGTTCCTAAT TGTGGCAACC CCACCATACA TCCGGGGCAG 
TGTTGCCCAT CATGTCCAGA TGAAATAGTT GTGCAGAAGC CAGAGCTCAC TAGTCCATCA 
ATCTGTCATG CTCCTGGTGG GGAATACTTT GTAGAAGGAG AAACATGGAA TATTGATACT 
TGCACACAAT GTACATGCCA CAGCGGACGA GTCCTGTGTG AGACTGAAGT CTGTCCTCCT 
CTTCTTTGTC AAAACCCCAC TCGCACACAG GACTCCTGCT GTCCTCAGTG CCCAGATGAG 
CCTCTTCAGC CTTCGTCAAG CAATGTCAGC ATGCCCAGTT ACTGCAAAAA TGATGAAGGA 
GATATTTTTC TGACAGCTGA GTCCTGGAAG CCCAACGTCT GCACCAGCTG CATCTGCATG 
GATGGTGTAA TTAGATGCTA CTCTGAGTCT TGTCCACCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAGG GACAGTGTTG TCCTTACTGT ATTGAAGATA CAGTACCAAA GAAAGTGGTC 
TGCCACTTCA ATGGAAAAAC ATATGCAGAT GAGGAACGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACTCTT TGCTCCACTG TCAGCTGCCC ACCCCTCCCC 
TGTGCTGAAC CCATCAATGT GGAGGGAAGC TGCTGTCCCA TGTGCCCAGA GATGTATGTA 
CCTGAACCTA CTAACATCCC CATTGAGAAG ACTAATCATC GAGGAGACGT TGATCTAGAA 
GTGCCAAACT GGTCAACGCC TAGTGAAAAT GACATCATCC ACATTCACAG AGACATGAAT 
CATCTCCAGG GAGAGTACAG AAGTGGAAAT GGGCCACACC CCAGTGAAGA TGCGTCAGTT 
AGCTCTGTTG CCTTGGTGAC AATTCCAATC ACGATAGCGC TGTTAGTAAT CATCATCTTT 
CTGCTCATCA ATCAGAAAAA GCAGTGGATT CCAGTGTCCT GCTACAAAGC TCCAACAAAG 
CCATCTTGCC TAAACAATCA GCTGGTGTAT GTGGACTGCA AGAAAGGTAC TATGGTCCAG 
GTGGACAGTT CTCAGAGGAT GCTAAGAATT GCAGACCCGG ATTCAAGATA CAGCGGATTT 
TACAGCATGC AGAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA