DENND5B cDNA ORF clone, Chrysochloris asiatica(Cape golden mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND5B
Entrez Gene ID	102821246
Full Name	DENN domain containing 5B
Gene Type	protein-coding
Organism	Chrysochloris asiatica(Cape golden mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006874750.1	XP_006874812.1	DENN domain-containing protein 5B isoform X2
XM_006874749.1	XP_006874811.1	DENN domain-containing protein 5B isoform X1

Pathways from BioSystems
Gene Ontology

The following DENND5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh55307	XM_006874750.1 Latest version!	Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh55306	XM_006874749.1 Latest version!	Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND5B ( XM_006874750.1 ) cDNA ORF clone, Chrysochloris asiatica(Cape golden mole) -> XP_006874812.1 Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh55307
Clone ID Related Accession (Same CDS sequence)	XM_006874750.1
Accession Version	XM_006874750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-18
Organism	Chrysochloris asiatica(Cape golden mole)
Product	DENN domain-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006408817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chrysochloris asiatica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGTA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAATAA AGATCCTCAG 
TTCCACTCAT TTATAATTAC CCGTGAAGAT GGTTCTCGTA CCTACGGTTT TGTTCTCACT 
TTTTATGAAG AAGTTACAAG CAAGCAAATC TGCACAGCAA TGCAGACGCT CTACCAGATG 
CACAATGCTG AGCAGTACAG CAGTGTGTAT GCGTCATCCT CCTGCAGCAT GGACTCGCTG 
GCAAGTAGTA TTGATGAGGG AGATGCAACT TCTCTTTTGA AACTCCAGAG ATACAACTCC 
TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGCTTGAT CACACCGCTG 
CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAGCTTT ATGAGGCAGT CACCTCACAG 
CAGCCTCCAC CCTTGCCGCT TGAGAGCTAC ATCCATAATA TTCTTTATGA GGTACCTCTT 
CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT TTGCCAGAGG 
CCTGGGCCCA GTGAACTCCC TCTCTCGGAT TACCCGCTTC GGGAGGCCTT CGAGCTCCTG 
GGATTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTT TAGAGATGCA GATACTTCTC 
TACTCACAAG ATTATCAACG CCTGATGACT GTGGCAGAAG GTATCACCAC ACTTTTGTTC 
CCATTTCAAT GGCAGCATGT TTATGTACCC ATCCTCCCTG CTTCTCTGCT ACATTTTCTT 
GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAA 
CTAGAACTTC CTCAAGAGGC TAATTTGTGT TTTGTGGACA TTGACAACCA TTTTATTGAA 
TTGCCTGAAG AATTTCCGCA GTTCCCCAAT AAGATGGATT TCATCCAGGA ACTCTCTGAA 
GTACTTCTTC AATTTGGGAT TCCTCCTGAA GGTAGCATAC ACTGCAGCGA AAGTGCCACC 
AAATTGAAGA ATCTGGTTCT GAAAGACTTG GTCAATGACA AAAAGAATGG CAATGTTTCC 
ACTAATAACA TCAGCATGTA TGAGTTACTT AAGGGAAATG AAACCATCGC CCGCCTCCAG 
GCTCTAGCTA AGAGAACTGG TGTGACTGTG GAAAAAATGG ACCTCTCTGC CACTCTGGAT 
GAAAAAGAAA AAGATGTAAA ACTTCACTGT GAGGAGGCAG AACTGAGGGA CTACAAGCTC 
AATGTCCAGC TCCGAGAGGT CTTTGCTAAC CGGTTTACGC AGATGTTTTC AGATTATGAA 
GCTTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TAACCAATCG GGAACAGATG 
CAGAACTTTG ACAAAGCTTC CTTTCTGTCT GACCAGCCTG AGCCTTACCT ACCATTTCTT 
TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAATAAAAT TATGTCTCAG 
TGGGAAGAGA AAGATCCCTT GCTTCGAGTC TTTGACTCTC GTATTGAGAA GATAAGACTG 
TATAATGTAA GGGCGCCCAC CATGCGGACG TCTGTCTATC AGAAATGCAG CACTTTAAAA 
GAAGCAGCCC AATCGATTGA GCAGCGACTG ATGAAAATGG ATCACACGGC AATCCACCCC 
CATCTACTAG ATATGAAAAT TGGTCAAGGC AAATATGAGC GTGGGTTCTT CCCAAAGTTA 
CAGGCCGATG TCTTGGCAAC AGGACCAGCC AATAACAACC GCTGGATAAG TCGGGGTGCC 
ACCACACAGC GCAGGAAAGA ACGCCTTCGC CAGCATTCTG AGCACATTGG AATAGACAAC 
GACCTGAGAG AGAAATATAT GCAAGAAGCA CGAAGTTTAG GAAAAAACCT GAGACAACCC 
AAATTGTCAG ACCTCTCTCC TGCAGTGATT GCACAGACCA ACTGGAAATT TGTCGAAGGC 
TTATTAAAAG AGTGTAGAAT GAAGACAAAG CGCATGTTAG TGGAGAAGAT GGGACATGAA 
GCAGTGGAAC TGGGTCATGG AGAAGCAAAC ATAACCGGCT TGGAAGAGAA TACCTTGATC 
GCCAGCCTCT GTGACCTACT GGAAAGGCTA TGGAGCCACG GCTTGCAGAT CAAGCAGGGG 
AAGTCGGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAAAA ACAAGAACAC 
CTTGCAGAAT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG 
TTACCAACAC TTAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG 
ATCAAGACTG ATGTTGGACG AGCTCGGGCG TGGATCAGAT TGTCTCTAGA AAAGAAGCTC 
TTGTCCCAGC ATCTCAAACA GTTGCTCTCT AACCAGCCAC TCACCAAGAA GCTTTATAAG 
CGGTATGCTT TTCTACGCTG TGAAGAAGAA AGAGAACAGT TTCTTTACCA TCTCCTTTCC 
CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTGTTCA CCACTATCAT GATTCCATAT 
AGGTCAGTGA TCATCCCAAT CAAAAAGTTG AGCAATGCGA TCATCACATC TAACCCTTGG 
ATTTGTGTGG CAGGAGAGCT GGGAGATACA GGAGTAATGC AGATTCCAAA AAACCTCCTT 
GAAATGACTT TTGAGTGTCA GAACTTGGGG AAGCTGACCA CTGTGCAGAT TGGTCATGAC 
AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA 
GGACATACCT ACAGGTTCCC ATGTGGACGA TGGCTGGGGA AGGGTATTGA TGATGGAAGC 
CTGGAGAGAA TTCTCATCGG GGAATTGATG CCCTCTGCAT CAGATGAGGA CTTGGCAAAG 
CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACCTCAG CTAGGAGATT AAGCATTACT 
TCACTGACAG GAAAAACTAG CAAGCCTAAC ACTGGACAGA TCCAAGAAGG AATTGGAGAA 
GCTGTGAACA ATATTGTGAA ACATTTTCAC AAACCTGAGA AAGAGAGAGG AAGCCTCACA 
GTATTGCTTT GTGGAGAAAA TGGCCTGGTT GCAGCACTTG AGCAAGTTTT CCACCACGGG 
TTCAAATCCG CCCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTTAT AGAGAGAGCT 
GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGACAATG AAGATGATGT CCTTCTTCAG 
AAATCATCCT GTAAAACCTT CTGCCACTAT GTGCACGCTA TTAATACAGC ACCCAGGAAT 
ATTGGAAAGG ACGGCAAGTT CCAGATTTTA GTATGCCTCG GAACACGGGA TCGCCTGCTC 
CCACAGTGGA TTCCATTATT AGCTGAGTGT CCTGCCATTA CCCGAATGTA TGAAGAGAGC 
GCCCTCCTGC GAGACCACAG GACTGTCAAC TCCCTCATCC GCATTCTGCA GACTATTCAG 
GACTTTACCA TCATCCTAGA AGGATCCCTT ATCAAAGGAG TGGATGTGAA TCCAATTGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006874812.1
CDS	168..3770
Translation	MLCMPKGLSFRTQTDNKDPQFHSFIITREDGSRTYGFVLTFYEEVTSKQICTAMQTLYQMHNAEQYSSVYASSSCSMDSLASSIDEGDATSLLKLQRYNSYDISRDTLYVSKSICLITPLPFMQACKKFLIQLYEAVTSQQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEPVICQRPGPSELPLSDYPLREAFELLGLENLVQVFTCVLLEMQILLYSQDYQRLMTVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKMDFIQELSEVLLQFGIPPEGSIHCSESATKLKNLVLKDLVNDKKNGNVSTNNISMYELLKGNETIARLQALAKRTGVTVEKMDLSATLDEKEKDVKLHCEEAELRDYKLNVQLREVFANRFTQMFSDYEAFVIQTAQDLESWLTNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSQWEEKDPLLRVFDSRIEKIRLYNVRAPTMRTSVYQKCSTLKEAAQSIEQRLMKMDHTAIHPHLLDMKIGQGKYERGFFPKLQADVLATGPANNNRWISRGATTQRRKERLRQHSEHIGIDNDLREKYMQEARSLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERLWSHGLQIKQGKSALWSHLIQFQDREEKQEHLAESPVALGPERRKSDSGVMLPTLRVSLIQDMRHIQNMSEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQPLTKKLYKRYAFLRCEEEREQFLYHLLSLNAVDYFCFTSVFTTIMIPYRSVIIPIKKLSNAIITSNPWICVAGELGDTGVMQIPKNLLEMTFECQNLGKLTTVQIGHDNSGLLAKWLVDCVMVRNEITGHTYRFPCGRWLGKGIDDGSLERILIGELMPSASDEDLAKQCRTPPQQKSPTSARRLSITSLTGKTSKPNTGQIQEGIGEAVNNIVKHFHKPEKERGSLTVLLCGENGLVAALEQVFHHGFKSARIFHKNVFIWDFIERAVAYFETTDQILDNEDDVLLQKSSCKTFCHYVHAINTAPRNIGKDGKFQILVCLGTRDRLLPQWIPLLAECPAITRMYEESALLRDHRTVNSLIRILQTIQDFTIILEGSLIKGVDVNPIG

Target ORF information:

RefSeq Version	XM_006874750.1
Organism	Chrysochloris asiatica(Cape golden mole)
Definition	Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006874750.1

ORF Insert Sequence:

ATGCTGTGTA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAATAA AGATCCTCAG 
TTCCACTCAT TTATAATTAC CCGTGAAGAT GGTTCTCGTA CCTACGGTTT TGTTCTCACT 
TTTTATGAAG AAGTTACAAG CAAGCAAATC TGCACAGCAA TGCAGACGCT CTACCAGATG 
CACAATGCTG AGCAGTACAG CAGTGTGTAT GCGTCATCCT CCTGCAGCAT GGACTCGCTG 
GCAAGTAGTA TTGATGAGGG AGATGCAACT TCTCTTTTGA AACTCCAGAG ATACAACTCC 
TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGCTTGAT CACACCGCTG 
CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAGCTTT ATGAGGCAGT CACCTCACAG 
CAGCCTCCAC CCTTGCCGCT TGAGAGCTAC ATCCATAATA TTCTTTATGA GGTACCTCTT 
CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT TTGCCAGAGG 
CCTGGGCCCA GTGAACTCCC TCTCTCGGAT TACCCGCTTC GGGAGGCCTT CGAGCTCCTG 
GGATTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTT TAGAGATGCA GATACTTCTC 
TACTCACAAG ATTATCAACG CCTGATGACT GTGGCAGAAG GTATCACCAC ACTTTTGTTC 
CCATTTCAAT GGCAGCATGT TTATGTACCC ATCCTCCCTG CTTCTCTGCT ACATTTTCTT 
GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAA 
CTAGAACTTC CTCAAGAGGC TAATTTGTGT TTTGTGGACA TTGACAACCA TTTTATTGAA 
TTGCCTGAAG AATTTCCGCA GTTCCCCAAT AAGATGGATT TCATCCAGGA ACTCTCTGAA 
GTACTTCTTC AATTTGGGAT TCCTCCTGAA GGTAGCATAC ACTGCAGCGA AAGTGCCACC 
AAATTGAAGA ATCTGGTTCT GAAAGACTTG GTCAATGACA AAAAGAATGG CAATGTTTCC 
ACTAATAACA TCAGCATGTA TGAGTTACTT AAGGGAAATG AAACCATCGC CCGCCTCCAG 
GCTCTAGCTA AGAGAACTGG TGTGACTGTG GAAAAAATGG ACCTCTCTGC CACTCTGGAT 
GAAAAAGAAA AAGATGTAAA ACTTCACTGT GAGGAGGCAG AACTGAGGGA CTACAAGCTC 
AATGTCCAGC TCCGAGAGGT CTTTGCTAAC CGGTTTACGC AGATGTTTTC AGATTATGAA 
GCTTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TAACCAATCG GGAACAGATG 
CAGAACTTTG ACAAAGCTTC CTTTCTGTCT GACCAGCCTG AGCCTTACCT ACCATTTCTT 
TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAATAAAAT TATGTCTCAG 
TGGGAAGAGA AAGATCCCTT GCTTCGAGTC TTTGACTCTC GTATTGAGAA GATAAGACTG 
TATAATGTAA GGGCGCCCAC CATGCGGACG TCTGTCTATC AGAAATGCAG CACTTTAAAA 
GAAGCAGCCC AATCGATTGA GCAGCGACTG ATGAAAATGG ATCACACGGC AATCCACCCC 
CATCTACTAG ATATGAAAAT TGGTCAAGGC AAATATGAGC GTGGGTTCTT CCCAAAGTTA 
CAGGCCGATG TCTTGGCAAC AGGACCAGCC AATAACAACC GCTGGATAAG TCGGGGTGCC 
ACCACACAGC GCAGGAAAGA ACGCCTTCGC CAGCATTCTG AGCACATTGG AATAGACAAC 
GACCTGAGAG AGAAATATAT GCAAGAAGCA CGAAGTTTAG GAAAAAACCT GAGACAACCC 
AAATTGTCAG ACCTCTCTCC TGCAGTGATT GCACAGACCA ACTGGAAATT TGTCGAAGGC 
TTATTAAAAG AGTGTAGAAT GAAGACAAAG CGCATGTTAG TGGAGAAGAT GGGACATGAA 
GCAGTGGAAC TGGGTCATGG AGAAGCAAAC ATAACCGGCT TGGAAGAGAA TACCTTGATC 
GCCAGCCTCT GTGACCTACT GGAAAGGCTA TGGAGCCACG GCTTGCAGAT CAAGCAGGGG 
AAGTCGGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAAAA ACAAGAACAC 
CTTGCAGAAT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG 
TTACCAACAC TTAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG 
ATCAAGACTG ATGTTGGACG AGCTCGGGCG TGGATCAGAT TGTCTCTAGA AAAGAAGCTC 
TTGTCCCAGC ATCTCAAACA GTTGCTCTCT AACCAGCCAC TCACCAAGAA GCTTTATAAG 
CGGTATGCTT TTCTACGCTG TGAAGAAGAA AGAGAACAGT TTCTTTACCA TCTCCTTTCC 
CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTGTTCA CCACTATCAT GATTCCATAT 
AGGTCAGTGA TCATCCCAAT CAAAAAGTTG AGCAATGCGA TCATCACATC TAACCCTTGG 
ATTTGTGTGG CAGGAGAGCT GGGAGATACA GGAGTAATGC AGATTCCAAA AAACCTCCTT 
GAAATGACTT TTGAGTGTCA GAACTTGGGG AAGCTGACCA CTGTGCAGAT TGGTCATGAC 
AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA 
GGACATACCT ACAGGTTCCC ATGTGGACGA TGGCTGGGGA AGGGTATTGA TGATGGAAGC 
CTGGAGAGAA TTCTCATCGG GGAATTGATG CCCTCTGCAT CAGATGAGGA CTTGGCAAAG 
CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACCTCAG CTAGGAGATT AAGCATTACT 
TCACTGACAG GAAAAACTAG CAAGCCTAAC ACTGGACAGA TCCAAGAAGG AATTGGAGAA 
GCTGTGAACA ATATTGTGAA ACATTTTCAC AAACCTGAGA AAGAGAGAGG AAGCCTCACA 
GTATTGCTTT GTGGAGAAAA TGGCCTGGTT GCAGCACTTG AGCAAGTTTT CCACCACGGG 
TTCAAATCCG CCCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTTAT AGAGAGAGCT 
GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGACAATG AAGATGATGT CCTTCTTCAG 
AAATCATCCT GTAAAACCTT CTGCCACTAT GTGCACGCTA TTAATACAGC ACCCAGGAAT 
ATTGGAAAGG ACGGCAAGTT CCAGATTTTA GTATGCCTCG GAACACGGGA TCGCCTGCTC 
CCACAGTGGA TTCCATTATT AGCTGAGTGT CCTGCCATTA CCCGAATGTA TGAAGAGAGC 
GCCCTCCTGC GAGACCACAG GACTGTCAAC TCCCTCATCC GCATTCTGCA GACTATTCAG 
GACTTTACCA TCATCCTAGA AGGATCCCTT ATCAAAGGAG TGGATGTGAA TCCAATTGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5B ( XM_006874749.1 ) cDNA ORF clone, Chrysochloris asiatica(Cape golden mole) -> XP_006874811.1 Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh55306
Clone ID Related Accession (Same CDS sequence)	XM_006874749.1
Accession Version	XM_006874749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-18
Organism	Chrysochloris asiatica(Cape golden mole)
Product	DENN domain-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006408817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chrysochloris asiatica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGA GCGGCGCGGC GCCGGGGCCG GGCTCGGGCT CCTCCCCAGC CGCCTGCCGC 
TTCGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCCGACGAG 
CTGGCGGGTG AAAATTTTGA CCAGAGTCCT CTGAGAAGAA CATTTAAATC CAAAGTTCTG 
GCCCACTATC CTCAGAATAT AGAATGGAAT CCTTTTGATC AAGATGCTGT GAACATGCTG 
TGTATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ATAAAGATCC TCAGTTCCAC 
TCATTTATAA TTACCCGTGA AGATGGTTCT CGTACCTACG GTTTTGTTCT CACTTTTTAT 
GAAGAAGTTA CAAGCAAGCA AATCTGCACA GCAATGCAGA CGCTCTACCA GATGCACAAT 
GCTGAGCAGT ACAGCAGTGT GTATGCGTCA TCCTCCTGCA GCATGGACTC GCTGGCAAGT 
AGTATTGATG AGGGAGATGC AACTTCTCTT TTGAAACTCC AGAGATACAA CTCCTATGAC 
ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGCT TGATCACACC GCTGCCTTTC 
ATGCAGGCCT GCAAGAAATT CCTCATCCAG CTTTATGAGG CAGTCACCTC ACAGCAGCCT 
CCACCCTTGC CGCTTGAGAG CTACATCCAT AATATTCTTT ATGAGGTACC TCTTCCACCT 
CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATTTGCCA GAGGCCTGGG 
CCCAGTGAAC TCCCTCTCTC GGATTACCCG CTTCGGGAGG CCTTCGAGCT CCTGGGATTA 
GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTTTAGAGA TGCAGATACT TCTCTACTCA 
CAAGATTATC AACGCCTGAT GACTGTGGCA GAAGGTATCA CCACACTTTT GTTCCCATTT 
CAATGGCAGC ATGTTTATGT ACCCATCCTC CCTGCTTCTC TGCTACATTT TCTTGATGCT 
CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAACTAGAA 
CTTCCTCAAG AGGCTAATTT GTGTTTTGTG GACATTGACA ACCATTTTAT TGAATTGCCT 
GAAGAATTTC CGCAGTTCCC CAATAAGATG GATTTCATCC AGGAACTCTC TGAAGTACTT 
CTTCAATTTG GGATTCCTCC TGAAGGTAGC ATACACTGCA GCGAAAGTGC CACCAAATTG 
AAGAATCTGG TTCTGAAAGA CTTGGTCAAT GACAAAAAGA ATGGCAATGT TTCCACTAAT 
AACATCAGCA TGTATGAGTT ACTTAAGGGA AATGAAACCA TCGCCCGCCT CCAGGCTCTA 
GCTAAGAGAA CTGGTGTGAC TGTGGAAAAA ATGGACCTCT CTGCCACTCT GGATGAAAAA 
GAAAAAGATG TAAAACTTCA CTGTGAGGAG GCAGAACTGA GGGACTACAA GCTCAATGTC 
CAGCTCCGAG AGGTCTTTGC TAACCGGTTT ACGCAGATGT TTTCAGATTA TGAAGCTTTT 
GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTAACCA ATCGGGAACA GATGCAGAAC 
TTTGACAAAG CTTCCTTTCT GTCTGACCAG CCTGAGCCTT ACCTACCATT TCTTTCACGC 
TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAATA AAATTATGTC TCAGTGGGAA 
GAGAAAGATC CCTTGCTTCG AGTCTTTGAC TCTCGTATTG AGAAGATAAG ACTGTATAAT 
GTAAGGGCGC CCACCATGCG GACGTCTGTC TATCAGAAAT GCAGCACTTT AAAAGAAGCA 
GCCCAATCGA TTGAGCAGCG ACTGATGAAA ATGGATCACA CGGCAATCCA CCCCCATCTA 
CTAGATATGA AAATTGGTCA AGGCAAATAT GAGCGTGGGT TCTTCCCAAA GTTACAGGCC 
GATGTCTTGG CAACAGGACC AGCCAATAAC AACCGCTGGA TAAGTCGGGG TGCCACCACA 
CAGCGCAGGA AAGAACGCCT TCGCCAGCAT TCTGAGCACA TTGGAATAGA CAACGACCTG 
AGAGAGAAAT ATATGCAAGA AGCACGAAGT TTAGGAAAAA ACCTGAGACA ACCCAAATTG 
TCAGACCTCT CTCCTGCAGT GATTGCACAG ACCAACTGGA AATTTGTCGA AGGCTTATTA 
AAAGAGTGTA GAATGAAGAC AAAGCGCATG TTAGTGGAGA AGATGGGACA TGAAGCAGTG 
GAACTGGGTC ATGGAGAAGC AAACATAACC GGCTTGGAAG AGAATACCTT GATCGCCAGC 
CTCTGTGACC TACTGGAAAG GCTATGGAGC CACGGCTTGC AGATCAAGCA GGGGAAGTCG 
GCTTTGTGGT CACATTTAAT TCAGTTTCAG GACAGAGAAG AAAAACAAGA ACACCTTGCA 
GAATCACCAG TTGCCCTTGG ACCAGAAAGA AGAAAATCTG ACTCAGGAGT TATGTTACCA 
ACACTTAGGG TCTCTCTTAT CCAAGACATG AGGCATATTC AAAACATGAG TGAGATCAAG 
ACTGATGTTG GACGAGCTCG GGCGTGGATC AGATTGTCTC TAGAAAAGAA GCTCTTGTCC 
CAGCATCTCA AACAGTTGCT CTCTAACCAG CCACTCACCA AGAAGCTTTA TAAGCGGTAT 
GCTTTTCTAC GCTGTGAAGA AGAAAGAGAA CAGTTTCTTT ACCATCTCCT TTCCCTCAAT 
GCTGTGGACT ATTTCTGCTT CACCAGTGTG TTCACCACTA TCATGATTCC ATATAGGTCA 
GTGATCATCC CAATCAAAAA GTTGAGCAAT GCGATCATCA CATCTAACCC TTGGATTTGT 
GTGGCAGGAG AGCTGGGAGA TACAGGAGTA ATGCAGATTC CAAAAAACCT CCTTGAAATG 
ACTTTTGAGT GTCAGAACTT GGGGAAGCTG ACCACTGTGC AGATTGGTCA TGACAACTCA 
GGACTGTTAG CCAAATGGCT AGTGGATTGT GTCATGGTCA GAAATGAAAT CACAGGACAT 
ACCTACAGGT TCCCATGTGG ACGATGGCTG GGGAAGGGTA TTGATGATGG AAGCCTGGAG 
AGAATTCTCA TCGGGGAATT GATGCCCTCT GCATCAGATG AGGACTTGGC AAAGCAGTGT 
CGGACTCCAC CCCAGCAGAA ATCACCCACC TCAGCTAGGA GATTAAGCAT TACTTCACTG 
ACAGGAAAAA CTAGCAAGCC TAACACTGGA CAGATCCAAG AAGGAATTGG AGAAGCTGTG 
AACAATATTG TGAAACATTT TCACAAACCT GAGAAAGAGA GAGGAAGCCT CACAGTATTG 
CTTTGTGGAG AAAATGGCCT GGTTGCAGCA CTTGAGCAAG TTTTCCACCA CGGGTTCAAA 
TCCGCCCGCA TCTTTCACAA GAATGTCTTC ATCTGGGACT TTATAGAGAG AGCTGTTGCT 
TATTTTGAAA CCACTGACCA GATTCTAGAC AATGAAGATG ATGTCCTTCT TCAGAAATCA 
TCCTGTAAAA CCTTCTGCCA CTATGTGCAC GCTATTAATA CAGCACCCAG GAATATTGGA 
AAGGACGGCA AGTTCCAGAT TTTAGTATGC CTCGGAACAC GGGATCGCCT GCTCCCACAG 
TGGATTCCAT TATTAGCTGA GTGTCCTGCC ATTACCCGAA TGTATGAAGA GAGCGCCCTC 
CTGCGAGACC ACAGGACTGT CAACTCCCTC ATCCGCATTC TGCAGACTAT TCAGGACTTT 
ACCATCATCC TAGAAGGATC CCTTATCAAA GGAGTGGATG TGAATCCAAT TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006874811.1
CDS	1..3837
Translation	MSGSGAAPGPGSGSSPAACRFAHYFVLCGIDADSGLEPDELAGENFDQSPLRRTFKSKVLAHYPQNIEWNPFDQDAVNMLCMPKGLSFRTQTDNKDPQFHSFIITREDGSRTYGFVLTFYEEVTSKQICTAMQTLYQMHNAEQYSSVYASSSCSMDSLASSIDEGDATSLLKLQRYNSYDISRDTLYVSKSICLITPLPFMQACKKFLIQLYEAVTSQQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEPVICQRPGPSELPLSDYPLREAFELLGLENLVQVFTCVLLEMQILLYSQDYQRLMTVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKMDFIQELSEVLLQFGIPPEGSIHCSESATKLKNLVLKDLVNDKKNGNVSTNNISMYELLKGNETIARLQALAKRTGVTVEKMDLSATLDEKEKDVKLHCEEAELRDYKLNVQLREVFANRFTQMFSDYEAFVIQTAQDLESWLTNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSQWEEKDPLLRVFDSRIEKIRLYNVRAPTMRTSVYQKCSTLKEAAQSIEQRLMKMDHTAIHPHLLDMKIGQGKYERGFFPKLQADVLATGPANNNRWISRGATTQRRKERLRQHSEHIGIDNDLREKYMQEARSLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERLWSHGLQIKQGKSALWSHLIQFQDREEKQEHLAESPVALGPERRKSDSGVMLPTLRVSLIQDMRHIQNMSEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQPLTKKLYKRYAFLRCEEEREQFLYHLLSLNAVDYFCFTSVFTTIMIPYRSVIIPIKKLSNAIITSNPWICVAGELGDTGVMQIPKNLLEMTFECQNLGKLTTVQIGHDNSGLLAKWLVDCVMVRNEITGHTYRFPCGRWLGKGIDDGSLERILIGELMPSASDEDLAKQCRTPPQQKSPTSARRLSITSLTGKTSKPNTGQIQEGIGEAVNNIVKHFHKPEKERGSLTVLLCGENGLVAALEQVFHHGFKSARIFHKNVFIWDFIERAVAYFETTDQILDNEDDVLLQKSSCKTFCHYVHAINTAPRNIGKDGKFQILVCLGTRDRLLPQWIPLLAECPAITRMYEESALLRDHRTVNSLIRILQTIQDFTIILEGSLIKGVDVNPIG

Target ORF information:

RefSeq Version	XM_006874749.1
Organism	Chrysochloris asiatica(Cape golden mole)
Definition	Chrysochloris asiatica DENN/MADD domain containing 5B (DENND5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006874749.1

ORF Insert Sequence:

ATGAGCGGGA GCGGCGCGGC GCCGGGGCCG GGCTCGGGCT CCTCCCCAGC CGCCTGCCGC 
TTCGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCCGACGAG 
CTGGCGGGTG AAAATTTTGA CCAGAGTCCT CTGAGAAGAA CATTTAAATC CAAAGTTCTG 
GCCCACTATC CTCAGAATAT AGAATGGAAT CCTTTTGATC AAGATGCTGT GAACATGCTG 
TGTATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ATAAAGATCC TCAGTTCCAC 
TCATTTATAA TTACCCGTGA AGATGGTTCT CGTACCTACG GTTTTGTTCT CACTTTTTAT 
GAAGAAGTTA CAAGCAAGCA AATCTGCACA GCAATGCAGA CGCTCTACCA GATGCACAAT 
GCTGAGCAGT ACAGCAGTGT GTATGCGTCA TCCTCCTGCA GCATGGACTC GCTGGCAAGT 
AGTATTGATG AGGGAGATGC AACTTCTCTT TTGAAACTCC AGAGATACAA CTCCTATGAC 
ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGCT TGATCACACC GCTGCCTTTC 
ATGCAGGCCT GCAAGAAATT CCTCATCCAG CTTTATGAGG CAGTCACCTC ACAGCAGCCT 
CCACCCTTGC CGCTTGAGAG CTACATCCAT AATATTCTTT ATGAGGTACC TCTTCCACCT 
CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATTTGCCA GAGGCCTGGG 
CCCAGTGAAC TCCCTCTCTC GGATTACCCG CTTCGGGAGG CCTTCGAGCT CCTGGGATTA 
GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTTTAGAGA TGCAGATACT TCTCTACTCA 
CAAGATTATC AACGCCTGAT GACTGTGGCA GAAGGTATCA CCACACTTTT GTTCCCATTT 
CAATGGCAGC ATGTTTATGT ACCCATCCTC CCTGCTTCTC TGCTACATTT TCTTGATGCT 
CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAACTAGAA 
CTTCCTCAAG AGGCTAATTT GTGTTTTGTG GACATTGACA ACCATTTTAT TGAATTGCCT 
GAAGAATTTC CGCAGTTCCC CAATAAGATG GATTTCATCC AGGAACTCTC TGAAGTACTT 
CTTCAATTTG GGATTCCTCC TGAAGGTAGC ATACACTGCA GCGAAAGTGC CACCAAATTG 
AAGAATCTGG TTCTGAAAGA CTTGGTCAAT GACAAAAAGA ATGGCAATGT TTCCACTAAT 
AACATCAGCA TGTATGAGTT ACTTAAGGGA AATGAAACCA TCGCCCGCCT CCAGGCTCTA 
GCTAAGAGAA CTGGTGTGAC TGTGGAAAAA ATGGACCTCT CTGCCACTCT GGATGAAAAA 
GAAAAAGATG TAAAACTTCA CTGTGAGGAG GCAGAACTGA GGGACTACAA GCTCAATGTC 
CAGCTCCGAG AGGTCTTTGC TAACCGGTTT ACGCAGATGT TTTCAGATTA TGAAGCTTTT 
GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTAACCA ATCGGGAACA GATGCAGAAC 
TTTGACAAAG CTTCCTTTCT GTCTGACCAG CCTGAGCCTT ACCTACCATT TCTTTCACGC 
TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAATA AAATTATGTC TCAGTGGGAA 
GAGAAAGATC CCTTGCTTCG AGTCTTTGAC TCTCGTATTG AGAAGATAAG ACTGTATAAT 
GTAAGGGCGC CCACCATGCG GACGTCTGTC TATCAGAAAT GCAGCACTTT AAAAGAAGCA 
GCCCAATCGA TTGAGCAGCG ACTGATGAAA ATGGATCACA CGGCAATCCA CCCCCATCTA 
CTAGATATGA AAATTGGTCA AGGCAAATAT GAGCGTGGGT TCTTCCCAAA GTTACAGGCC 
GATGTCTTGG CAACAGGACC AGCCAATAAC AACCGCTGGA TAAGTCGGGG TGCCACCACA 
CAGCGCAGGA AAGAACGCCT TCGCCAGCAT TCTGAGCACA TTGGAATAGA CAACGACCTG 
AGAGAGAAAT ATATGCAAGA AGCACGAAGT TTAGGAAAAA ACCTGAGACA ACCCAAATTG 
TCAGACCTCT CTCCTGCAGT GATTGCACAG ACCAACTGGA AATTTGTCGA AGGCTTATTA 
AAAGAGTGTA GAATGAAGAC AAAGCGCATG TTAGTGGAGA AGATGGGACA TGAAGCAGTG 
GAACTGGGTC ATGGAGAAGC AAACATAACC GGCTTGGAAG AGAATACCTT GATCGCCAGC 
CTCTGTGACC TACTGGAAAG GCTATGGAGC CACGGCTTGC AGATCAAGCA GGGGAAGTCG 
GCTTTGTGGT CACATTTAAT TCAGTTTCAG GACAGAGAAG AAAAACAAGA ACACCTTGCA 
GAATCACCAG TTGCCCTTGG ACCAGAAAGA AGAAAATCTG ACTCAGGAGT TATGTTACCA 
ACACTTAGGG TCTCTCTTAT CCAAGACATG AGGCATATTC AAAACATGAG TGAGATCAAG 
ACTGATGTTG GACGAGCTCG GGCGTGGATC AGATTGTCTC TAGAAAAGAA GCTCTTGTCC 
CAGCATCTCA AACAGTTGCT CTCTAACCAG CCACTCACCA AGAAGCTTTA TAAGCGGTAT 
GCTTTTCTAC GCTGTGAAGA AGAAAGAGAA CAGTTTCTTT ACCATCTCCT TTCCCTCAAT 
GCTGTGGACT ATTTCTGCTT CACCAGTGTG TTCACCACTA TCATGATTCC ATATAGGTCA 
GTGATCATCC CAATCAAAAA GTTGAGCAAT GCGATCATCA CATCTAACCC TTGGATTTGT 
GTGGCAGGAG AGCTGGGAGA TACAGGAGTA ATGCAGATTC CAAAAAACCT CCTTGAAATG 
ACTTTTGAGT GTCAGAACTT GGGGAAGCTG ACCACTGTGC AGATTGGTCA TGACAACTCA 
GGACTGTTAG CCAAATGGCT AGTGGATTGT GTCATGGTCA GAAATGAAAT CACAGGACAT 
ACCTACAGGT TCCCATGTGG ACGATGGCTG GGGAAGGGTA TTGATGATGG AAGCCTGGAG 
AGAATTCTCA TCGGGGAATT GATGCCCTCT GCATCAGATG AGGACTTGGC AAAGCAGTGT 
CGGACTCCAC CCCAGCAGAA ATCACCCACC TCAGCTAGGA GATTAAGCAT TACTTCACTG 
ACAGGAAAAA CTAGCAAGCC TAACACTGGA CAGATCCAAG AAGGAATTGG AGAAGCTGTG 
AACAATATTG TGAAACATTT TCACAAACCT GAGAAAGAGA GAGGAAGCCT CACAGTATTG 
CTTTGTGGAG AAAATGGCCT GGTTGCAGCA CTTGAGCAAG TTTTCCACCA CGGGTTCAAA 
TCCGCCCGCA TCTTTCACAA GAATGTCTTC ATCTGGGACT TTATAGAGAG AGCTGTTGCT 
TATTTTGAAA CCACTGACCA GATTCTAGAC AATGAAGATG ATGTCCTTCT TCAGAAATCA 
TCCTGTAAAA CCTTCTGCCA CTATGTGCAC GCTATTAATA CAGCACCCAG GAATATTGGA 
AAGGACGGCA AGTTCCAGAT TTTAGTATGC CTCGGAACAC GGGATCGCCT GCTCCCACAG 
TGGATTCCAT TATTAGCTGA GTGTCCTGCC ATTACCCGAA TGTATGAAGA GAGCGCCCTC 
CTGCGAGACC ACAGGACTGT CAACTCCCTC ATCCGCATTC TGCAGACTAT TCAGGACTTT 
ACCATCATCC TAGAAGGATC CCTTATCAAA GGAGTGGATG TGAATCCAAT TGGCTAG