HCN1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCN1
Entrez Gene ID	612434
Full Name	hyperpolarization activated cyclic nucleotide gated potassium channel 1
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium channel 1 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_850162.3	XP_855255.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
XM_850162.4	XP_855255.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN1	NP_066550.2
Danio rerio (zebrafish)	LOC100334297	XP_005169491.1
Rattus norvegicus (Norway rat)	Hcn1	NP_445827.1
Mus musculus (house mouse)	Hcn1	NP_034538.2
Gallus gallus (chicken)	HCN1	XP_429145.3
Xenopus tropicalis (tropical clawed frog)	hcn1	XP_002933077.2
Canis lupus familiaris (dog)	HCN1	XP_855255.1
Bos taurus (cattle)	HCN1	NP_001193509.1

Gene Ontology

The following HCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe05464	XM_850162.4 Latest version!	Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCe05464	XM_850162.3	Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCN1 ( XM_850162.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_855255.1 Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Price & Availability↑

CloneID	OCe05464
Clone ID Related Accession (Same CDS sequence)	XM_850162.3 , XM_850162.4
Accession Version	XM_850162.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_850162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG GCGGCAAGCC CAACTCCGCG TCCAACAGCC GGGACGATGG CAGCAGCGCC 
TTCCCCGGCA AGGCGCCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GCGGCGGCGG CGCGGGCGCG AAGGAGCTCG GCAACTCGGT GTGCTTCAAG 
GTGGACGGCG GCGGCGCGGG CGAGGAGCCG GCCGGGGGCT TCGAGGACGC CGAGGGCCCC 
CGGCGGCAGT ACGGCTTCAT GCAGAGGCAG TTCACCTCCA TGCTGCAGCC CGGGGTCAAC 
AAATTTTCCC TCCGCATGTT CGGGAGCCAG AAGGCGGTGG AGAAGGAGCA GGAAAGGGTT 
AAAACTGCAG GCTTCTGGAT TATCCACCCT TACAGCGATT TCAGGTTTTA TTGGGATTTA 
ATAATGCTTA TCATGATGGT TGGAAATCTG GTCATCATAC CAGTTGGAAT CACATTCTTT 
ACAGAACAAA CAACAACACC ATGGATTATT TTCAATGTGG CATCAGATAC AGTTTTCCTT 
TTGGACCTGA TAATGAATTT TAGGACTGGC ACTGTCAATG AAGACAGTTC TGAAATCATC 
CTGGACCCTA AAGTGATCAA GATGAATTAT TTAAAAAGCT GGTTTGTGGT TGACTTTATC 
TCATCAATCC CAGTGGATTA TATCTTTCTC ATTGTAGAAA AAGGAATGGA TTCAGAAGTT 
TACAAGACAG CCAGGGCACT TCGCATTGTG AGGTTTACAA AAATTCTCAG TCTCTTGCGT 
TTATTACGAC TTTCAAGGTT AATTAGATAC ATACATCAGT GGGAAGAGAT TTTCCACATG 
ACGTATGACC TTGCCAGTGC AGTGGTGAGA ATATTTAACC TCATCGGCAT GATGCTGCTC 
CTATGCCACT GGGATGGCTG TCTTCAGTTC TTAGTACCAC TACTGCAGGA TTTCCCACCA 
GATTGCTGGG TGTCTCTGAA TGAAATGGTT AATGATTCAT GGGGAAAGCA GTATTCATAT 
GCACTCTTCA AAGCTATGAG TCACATGTTG TGCATCGGGT ATGGAGCCCA GGCCCCAGTC 
AGCATGTCTG ACCTCTGGAT TACCATGCTG AGCATGATTG TTGGGGCCAC CTGCTATGCC 
ATGTTTGTTG GCCACGCCAC AGCTTTAATC CAGTCTTTGG ATTCCTCAAG ACGGCAGTAC 
CAAGAGAAGT ACAAACAAGT GGAACAATAC ATGTCATTCC ATAAGTTACC AGCTGATATG 
CGTCAGAAGA TACATGATTA CTATGAACAC AGATACCAAG GCAAAATCTT TGATGAAGAA 
AACATTCTCA ATGAACTCAA TGATCCTCTG AGAGAGGAGA TAGTCAACTT CAACTGTCGG 
AAGCTAGTGG CTACAATGCC ACTGTTTGCT AATGCGGATC CCAATTTTGT GACTGCCATG 
CTGAGCAAGT TGAGATTTGA GGTGTTTCAA CCTGGAGATT ATATCATACG AGAAGGAGCT 
GTGGGTAAAA AAATGTATTT CATTCAACAT GGTGTTGCTG GTGTCATCAC AAAATCCAGT 
AAGGAAATGA AGCTGACAGA TGGCTCTTAC TTTGGAGAGA TTTGCTTGCT GACCAAGGGA 
CGCCGTACTG CCAGTGTTCG AGCTGATACA TATTGCCGTC TTTATTCACT TTCTGTGGAC 
AATTTCAATG AGGTCCTGGA GGAATATCCA ATGATGAGGA GAGCCTTTGA GACGGTTGCC 
ATTGACCGAC TAGATCGGAT AGGGAAGAAA AATTCAATTC TGCTGCAAAA GTTCCAGAAG 
GATCTGAACA CCGGGGTGTT CAACAATCAG GAGAACGAGA TCCTGAAGCA GATCGTGAAG 
CACGACCGGG AGATGGTGCA GGCGATCGCT CCGATCAACT ACCCGCAGAT GACAGCCCTG 
AACTCCACCT CCTCCACCGC GACTCCGACC TGCCGCGCGA GGACGCAGTC CCCGCCGGTG 
TACTCCGCGC CCAGCCTGTC GCACGGCAAC CTGCACTCCC CCAGCCCCGG CACCCAGACC 
CCGCAGCCCT CCGCCGTGCT CTCGCCCTGC TCCTACACCA CCGCGGTGTG CAGCCCGCCC 
GTCCAGAGCC CGCTGGCCGC TCGAACTTTC CACTACGCCT CCCCCACGGC GTCCCAGCTG 
TCGCTCGCGC AGCAGCCGCA GCCCGCGCAG CCCCCGCAGC CCCCGCAGCC CGCGCAGCCC 
GCGCAGCCCC CGCAGCCGCA GCCCCCGCAG CCGCAGCCGC AGCCGCCGCA GCCCCCGCAG 
CCCCCGCAGC CGCCGCAGAC GCCCGGCGGC GGCTCCACGC CGAGGAACGA CGTGCACAGG 
AGCACCCAGG CGCTCCACAA CGCCAGCCTG ACCCGCGAGG TCAGGCCCCT GTCGGCCTCG 
CAGCCCTCGC TGCCCCACGA GGTTGCCACG CTCATGTCCA GACCGCACCC CACTGTGGGC 
GAGTCCCTGG CCTCCATCCC TCAGCCCGTG ACAGCCGTCC ACGGCCCGGG CCTGCAGGCG 
GGGGGCAGGG GCACCGTCCC GCAGCGAGTC ACCCTGTTCC GACAGATGTC CTCGGGAGCC 
ATCCCCCCGA ACCGAGGAGC GCCCCCCGCG CCCCCTCCAC CAGCAGCGGC TCTCCCCAGA 
GAGTCCTCCT CAGTCTTAAC TACAGACCCA GAGGCAGAAA AGCCACGGTT TGCTTCGAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_855255.1
CDS	76..2781
Translation	MEAGGKPNSASNSRDDGSSAFPGKAPATGAGPAAAEKRLGTPPGGGGAGAKELGNSVCFKVDGGGAGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTALNSTSSTATPTCRARTQSPPVYSAPSLSHGNLHSPSPGTQTPQPSAVLSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLAQQPQPAQPPQPPQPAQPAQPPQPQPPQPQPQPPQPPQPPQPPQTPGGGSTPRNDVHRSTQALHNASLTREVRPLSASQPSLPHEVATLMSRPHPTVGESLASIPQPVTAVHGPGLQAGGRGTVPQRVTLFRQMSSGAIPPNRGAPPAPPPPAAALPRESSSVLTTDPEAEKPRFASNL

Target ORF information:

RefSeq Version	XM_850162.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_850162.3

ORF Insert Sequence:

ATGGAAGCAG GCGGCAAGCC CAACTCCGCG TCCAACAGCC GGGACGATGG CAGCAGCGCC 
TTCCCCGGCA AGGCGCCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GCGGCGGCGG CGCGGGCGCG AAGGAGCTCG GCAACTCGGT GTGCTTCAAG 
GTGGACGGCG GCGGCGCGGG CGAGGAGCCG GCCGGGGGCT TCGAGGACGC CGAGGGCCCC 
CGGCGGCAGT ACGGCTTCAT GCAGAGGCAG TTCACCTCCA TGCTGCAGCC CGGGGTCAAC 
AAATTTTCCC TCCGCATGTT CGGGAGCCAG AAGGCGGTGG AGAAGGAGCA GGAAAGGGTT 
AAAACTGCAG GCTTCTGGAT TATCCACCCT TACAGCGATT TCAGGTTTTA TTGGGATTTA 
ATAATGCTTA TCATGATGGT TGGAAATCTG GTCATCATAC CAGTTGGAAT CACATTCTTT 
ACAGAACAAA CAACAACACC ATGGATTATT TTCAATGTGG CATCAGATAC AGTTTTCCTT 
TTGGACCTGA TAATGAATTT TAGGACTGGC ACTGTCAATG AAGACAGTTC TGAAATCATC 
CTGGACCCTA AAGTGATCAA GATGAATTAT TTAAAAAGCT GGTTTGTGGT TGACTTTATC 
TCATCAATCC CAGTGGATTA TATCTTTCTC ATTGTAGAAA AAGGAATGGA TTCAGAAGTT 
TACAAGACAG CCAGGGCACT TCGCATTGTG AGGTTTACAA AAATTCTCAG TCTCTTGCGT 
TTATTACGAC TTTCAAGGTT AATTAGATAC ATACATCAGT GGGAAGAGAT TTTCCACATG 
ACGTATGACC TTGCCAGTGC AGTGGTGAGA ATATTTAACC TCATCGGCAT GATGCTGCTC 
CTATGCCACT GGGATGGCTG TCTTCAGTTC TTAGTACCAC TACTGCAGGA TTTCCCACCA 
GATTGCTGGG TGTCTCTGAA TGAAATGGTT AATGATTCAT GGGGAAAGCA GTATTCATAT 
GCACTCTTCA AAGCTATGAG TCACATGTTG TGCATCGGGT ATGGAGCCCA GGCCCCAGTC 
AGCATGTCTG ACCTCTGGAT TACCATGCTG AGCATGATTG TTGGGGCCAC CTGCTATGCC 
ATGTTTGTTG GCCACGCCAC AGCTTTAATC CAGTCTTTGG ATTCCTCAAG ACGGCAGTAC 
CAAGAGAAGT ACAAACAAGT GGAACAATAC ATGTCATTCC ATAAGTTACC AGCTGATATG 
CGTCAGAAGA TACATGATTA CTATGAACAC AGATACCAAG GCAAAATCTT TGATGAAGAA 
AACATTCTCA ATGAACTCAA TGATCCTCTG AGAGAGGAGA TAGTCAACTT CAACTGTCGG 
AAGCTAGTGG CTACAATGCC ACTGTTTGCT AATGCGGATC CCAATTTTGT GACTGCCATG 
CTGAGCAAGT TGAGATTTGA GGTGTTTCAA CCTGGAGATT ATATCATACG AGAAGGAGCT 
GTGGGTAAAA AAATGTATTT CATTCAACAT GGTGTTGCTG GTGTCATCAC AAAATCCAGT 
AAGGAAATGA AGCTGACAGA TGGCTCTTAC TTTGGAGAGA TTTGCTTGCT GACCAAGGGA 
CGCCGTACTG CCAGTGTTCG AGCTGATACA TATTGCCGTC TTTATTCACT TTCTGTGGAC 
AATTTCAATG AGGTCCTGGA GGAATATCCA ATGATGAGGA GAGCCTTTGA GACGGTTGCC 
ATTGACCGAC TAGATCGGAT AGGGAAGAAA AATTCAATTC TGCTGCAAAA GTTCCAGAAG 
GATCTGAACA CCGGGGTGTT CAACAATCAG GAGAACGAGA TCCTGAAGCA GATCGTGAAG 
CACGACCGGG AGATGGTGCA GGCGATCGCT CCGATCAACT ACCCGCAGAT GACAGCCCTG 
AACTCCACCT CCTCCACCGC GACTCCGACC TGCCGCGCGA GGACGCAGTC CCCGCCGGTG 
TACTCCGCGC CCAGCCTGTC GCACGGCAAC CTGCACTCCC CCAGCCCCGG CACCCAGACC 
CCGCAGCCCT CCGCCGTGCT CTCGCCCTGC TCCTACACCA CCGCGGTGTG CAGCCCGCCC 
GTCCAGAGCC CGCTGGCCGC TCGAACTTTC CACTACGCCT CCCCCACGGC GTCCCAGCTG 
TCGCTCGCGC AGCAGCCGCA GCCCGCGCAG CCCCCGCAGC CCCCGCAGCC CGCGCAGCCC 
GCGCAGCCCC CGCAGCCGCA GCCCCCGCAG CCGCAGCCGC AGCCGCCGCA GCCCCCGCAG 
CCCCCGCAGC CGCCGCAGAC GCCCGGCGGC GGCTCCACGC CGAGGAACGA CGTGCACAGG 
AGCACCCAGG CGCTCCACAA CGCCAGCCTG ACCCGCGAGG TCAGGCCCCT GTCGGCCTCG 
CAGCCCTCGC TGCCCCACGA GGTTGCCACG CTCATGTCCA GACCGCACCC CACTGTGGGC 
GAGTCCCTGG CCTCCATCCC TCAGCCCGTG ACAGCCGTCC ACGGCCCGGG CCTGCAGGCG 
GGGGGCAGGG GCACCGTCCC GCAGCGAGTC ACCCTGTTCC GACAGATGTC CTCGGGAGCC 
ATCCCCCCGA ACCGAGGAGC GCCCCCCGCG CCCCCTCCAC CAGCAGCGGC TCTCCCCAGA 
GAGTCCTCCT CAGTCTTAAC TACAGACCCA GAGGCAGAAA AGCCACGGTT TGCTTCGAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN1 ( XM_850162.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_855255.1 Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Price & Availability↑

CloneID	OCe05464
Clone ID Related Accession (Same CDS sequence)	XM_850162.3 , XM_850162.4
Accession Version	XM_850162.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_850162.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG GCGGCAAGCC CAACTCCGCG TCCAACAGCC GGGACGATGG CAGCAGCGCC 
TTCCCCGGCA AGGCGCCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GCGGCGGCGG CGCGGGCGCG AAGGAGCTCG GCAACTCGGT GTGCTTCAAG 
GTGGACGGCG GCGGCGCGGG CGAGGAGCCG GCCGGGGGCT TCGAGGACGC CGAGGGCCCC 
CGGCGGCAGT ACGGCTTCAT GCAGAGGCAG TTCACCTCCA TGCTGCAGCC CGGGGTCAAC 
AAATTTTCCC TCCGCATGTT CGGGAGCCAG AAGGCGGTGG AGAAGGAGCA GGAAAGGGTT 
AAAACTGCAG GCTTCTGGAT TATCCACCCT TACAGCGATT TCAGGTTTTA TTGGGATTTA 
ATAATGCTTA TCATGATGGT TGGAAATCTG GTCATCATAC CAGTTGGAAT CACATTCTTT 
ACAGAACAAA CAACAACACC ATGGATTATT TTCAATGTGG CATCAGATAC AGTTTTCCTT 
TTGGACCTGA TAATGAATTT TAGGACTGGC ACTGTCAATG AAGACAGTTC TGAAATCATC 
CTGGACCCTA AAGTGATCAA GATGAATTAT TTAAAAAGCT GGTTTGTGGT TGACTTTATC 
TCATCAATCC CAGTGGATTA TATCTTTCTC ATTGTAGAAA AAGGAATGGA TTCAGAAGTT 
TACAAGACAG CCAGGGCACT TCGCATTGTG AGGTTTACAA AAATTCTCAG TCTCTTGCGT 
TTATTACGAC TTTCAAGGTT AATTAGATAC ATACATCAGT GGGAAGAGAT TTTCCACATG 
ACGTATGACC TTGCCAGTGC AGTGGTGAGA ATATTTAACC TCATCGGCAT GATGCTGCTC 
CTATGCCACT GGGATGGCTG TCTTCAGTTC TTAGTACCAC TACTGCAGGA TTTCCCACCA 
GATTGCTGGG TGTCTCTGAA TGAAATGGTT AATGATTCAT GGGGAAAGCA GTATTCATAT 
GCACTCTTCA AAGCTATGAG TCACATGTTG TGCATCGGGT ATGGAGCCCA GGCCCCAGTC 
AGCATGTCTG ACCTCTGGAT TACCATGCTG AGCATGATTG TTGGGGCCAC CTGCTATGCC 
ATGTTTGTTG GCCACGCCAC AGCTTTAATC CAGTCTTTGG ATTCCTCAAG ACGGCAGTAC 
CAAGAGAAGT ACAAACAAGT GGAACAATAC ATGTCATTCC ATAAGTTACC AGCTGATATG 
CGTCAGAAGA TACATGATTA CTATGAACAC AGATACCAAG GCAAAATCTT TGATGAAGAA 
AACATTCTCA ATGAACTCAA TGATCCTCTG AGAGAGGAGA TAGTCAACTT CAACTGTCGG 
AAGCTAGTGG CTACAATGCC ACTGTTTGCT AATGCGGATC CCAATTTTGT GACTGCCATG 
CTGAGCAAGT TGAGATTTGA GGTGTTTCAA CCTGGAGATT ATATCATACG AGAAGGAGCT 
GTGGGTAAAA AAATGTATTT CATTCAACAT GGTGTTGCTG GTGTCATCAC AAAATCCAGT 
AAGGAAATGA AGCTGACAGA TGGCTCTTAC TTTGGAGAGA TTTGCTTGCT GACCAAGGGA 
CGCCGTACTG CCAGTGTTCG AGCTGATACA TATTGCCGTC TTTATTCACT TTCTGTGGAC 
AATTTCAATG AGGTCCTGGA GGAATATCCA ATGATGAGGA GAGCCTTTGA GACGGTTGCC 
ATTGACCGAC TAGATCGGAT AGGGAAGAAA AATTCAATTC TGCTGCAAAA GTTCCAGAAG 
GATCTGAACA CCGGGGTGTT CAACAATCAG GAGAACGAGA TCCTGAAGCA GATCGTGAAG 
CACGACCGGG AGATGGTGCA GGCGATCGCT CCGATCAACT ACCCGCAGAT GACAGCCCTG 
AACTCCACCT CCTCCACCGC GACTCCGACC TGCCGCGCGA GGACGCAGTC CCCGCCGGTG 
TACTCCGCGC CCAGCCTGTC GCACGGCAAC CTGCACTCCC CCAGCCCCGG CACCCAGACC 
CCGCAGCCCT CCGCCGTGCT CTCGCCCTGC TCCTACACCA CCGCGGTGTG CAGCCCGCCC 
GTCCAGAGCC CGCTGGCCGC TCGAACTTTC CACTACGCCT CCCCCACGGC GTCCCAGCTG 
TCGCTCGCGC AGCAGCCGCA GCCCGCGCAG CCCCCGCAGC CCCCGCAGCC CGCGCAGCCC 
GCGCAGCCCC CGCAGCCGCA GCCCCCGCAG CCGCAGCCGC AGCCGCCGCA GCCCCCGCAG 
CCCCCGCAGC CGCCGCAGAC GCCCGGCGGC GGCTCCACGC CGAGGAACGA CGTGCACAGG 
AGCACCCAGG CGCTCCACAA CGCCAGCCTG ACCCGCGAGG TCAGGCCCCT GTCGGCCTCG 
CAGCCCTCGC TGCCCCACGA GGTTGCCACG CTCATGTCCA GACCGCACCC CACTGTGGGC 
GAGTCCCTGG CCTCCATCCC TCAGCCCGTG ACAGCCGTCC ACGGCCCGGG CCTGCAGGCG 
GGGGGCAGGG GCACCGTCCC GCAGCGAGTC ACCCTGTTCC GACAGATGTC CTCGGGAGCC 
ATCCCCCCGA ACCGAGGAGC GCCCCCCGCG CCCCCTCCAC CAGCAGCGGC TCTCCCCAGA 
GAGTCCTCCT CAGTCTTAAC TACAGACCCA GAGGCAGAAA AGCCACGGTT TGCTTCGAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_855255.1
CDS	1..2706
Translation	MEAGGKPNSASNSRDDGSSAFPGKAPATGAGPAAAEKRLGTPPGGGGAGAKELGNSVCFKVDGGGAGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTALNSTSSTATPTCRARTQSPPVYSAPSLSHGNLHSPSPGTQTPQPSAVLSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLAQQPQPAQPPQPPQPAQPAQPPQPQPPQPQPQPPQPPQPPQPPQTPGGGSTPRNDVHRSTQALHNASLTREVRPLSASQPSLPHEVATLMSRPHPTVGESLASIPQPVTAVHGPGLQAGGRGTVPQRVTLFRQMSSGAIPPNRGAPPAPPPPAAALPRESSSVLTTDPEAEKPRFASNL

Target ORF information:

RefSeq Version	XM_850162.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_850162.4

ORF Insert Sequence:

ATGGAAGCAG GCGGCAAGCC CAACTCCGCG TCCAACAGCC GGGACGATGG CAGCAGCGCC 
TTCCCCGGCA AGGCGCCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GCGGCGGCGG CGCGGGCGCG AAGGAGCTCG GCAACTCGGT GTGCTTCAAG 
GTGGACGGCG GCGGCGCGGG CGAGGAGCCG GCCGGGGGCT TCGAGGACGC CGAGGGCCCC 
CGGCGGCAGT ACGGCTTCAT GCAGAGGCAG TTCACCTCCA TGCTGCAGCC CGGGGTCAAC 
AAATTTTCCC TCCGCATGTT CGGGAGCCAG AAGGCGGTGG AGAAGGAGCA GGAAAGGGTT 
AAAACTGCAG GCTTCTGGAT TATCCACCCT TACAGCGATT TCAGGTTTTA TTGGGATTTA 
ATAATGCTTA TCATGATGGT TGGAAATCTG GTCATCATAC CAGTTGGAAT CACATTCTTT 
ACAGAACAAA CAACAACACC ATGGATTATT TTCAATGTGG CATCAGATAC AGTTTTCCTT 
TTGGACCTGA TAATGAATTT TAGGACTGGC ACTGTCAATG AAGACAGTTC TGAAATCATC 
CTGGACCCTA AAGTGATCAA GATGAATTAT TTAAAAAGCT GGTTTGTGGT TGACTTTATC 
TCATCAATCC CAGTGGATTA TATCTTTCTC ATTGTAGAAA AAGGAATGGA TTCAGAAGTT 
TACAAGACAG CCAGGGCACT TCGCATTGTG AGGTTTACAA AAATTCTCAG TCTCTTGCGT 
TTATTACGAC TTTCAAGGTT AATTAGATAC ATACATCAGT GGGAAGAGAT TTTCCACATG 
ACGTATGACC TTGCCAGTGC AGTGGTGAGA ATATTTAACC TCATCGGCAT GATGCTGCTC 
CTATGCCACT GGGATGGCTG TCTTCAGTTC TTAGTACCAC TACTGCAGGA TTTCCCACCA 
GATTGCTGGG TGTCTCTGAA TGAAATGGTT AATGATTCAT GGGGAAAGCA GTATTCATAT 
GCACTCTTCA AAGCTATGAG TCACATGTTG TGCATCGGGT ATGGAGCCCA GGCCCCAGTC 
AGCATGTCTG ACCTCTGGAT TACCATGCTG AGCATGATTG TTGGGGCCAC CTGCTATGCC 
ATGTTTGTTG GCCACGCCAC AGCTTTAATC CAGTCTTTGG ATTCCTCAAG ACGGCAGTAC 
CAAGAGAAGT ACAAACAAGT GGAACAATAC ATGTCATTCC ATAAGTTACC AGCTGATATG 
CGTCAGAAGA TACATGATTA CTATGAACAC AGATACCAAG GCAAAATCTT TGATGAAGAA 
AACATTCTCA ATGAACTCAA TGATCCTCTG AGAGAGGAGA TAGTCAACTT CAACTGTCGG 
AAGCTAGTGG CTACAATGCC ACTGTTTGCT AATGCGGATC CCAATTTTGT GACTGCCATG 
CTGAGCAAGT TGAGATTTGA GGTGTTTCAA CCTGGAGATT ATATCATACG AGAAGGAGCT 
GTGGGTAAAA AAATGTATTT CATTCAACAT GGTGTTGCTG GTGTCATCAC AAAATCCAGT 
AAGGAAATGA AGCTGACAGA TGGCTCTTAC TTTGGAGAGA TTTGCTTGCT GACCAAGGGA 
CGCCGTACTG CCAGTGTTCG AGCTGATACA TATTGCCGTC TTTATTCACT TTCTGTGGAC 
AATTTCAATG AGGTCCTGGA GGAATATCCA ATGATGAGGA GAGCCTTTGA GACGGTTGCC 
ATTGACCGAC TAGATCGGAT AGGGAAGAAA AATTCAATTC TGCTGCAAAA GTTCCAGAAG 
GATCTGAACA CCGGGGTGTT CAACAATCAG GAGAACGAGA TCCTGAAGCA GATCGTGAAG 
CACGACCGGG AGATGGTGCA GGCGATCGCT CCGATCAACT ACCCGCAGAT GACAGCCCTG 
AACTCCACCT CCTCCACCGC GACTCCGACC TGCCGCGCGA GGACGCAGTC CCCGCCGGTG 
TACTCCGCGC CCAGCCTGTC GCACGGCAAC CTGCACTCCC CCAGCCCCGG CACCCAGACC 
CCGCAGCCCT CCGCCGTGCT CTCGCCCTGC TCCTACACCA CCGCGGTGTG CAGCCCGCCC 
GTCCAGAGCC CGCTGGCCGC TCGAACTTTC CACTACGCCT CCCCCACGGC GTCCCAGCTG 
TCGCTCGCGC AGCAGCCGCA GCCCGCGCAG CCCCCGCAGC CCCCGCAGCC CGCGCAGCCC 
GCGCAGCCCC CGCAGCCGCA GCCCCCGCAG CCGCAGCCGC AGCCGCCGCA GCCCCCGCAG 
CCCCCGCAGC CGCCGCAGAC GCCCGGCGGC GGCTCCACGC CGAGGAACGA CGTGCACAGG 
AGCACCCAGG CGCTCCACAA CGCCAGCCTG ACCCGCGAGG TCAGGCCCCT GTCGGCCTCG 
CAGCCCTCGC TGCCCCACGA GGTTGCCACG CTCATGTCCA GACCGCACCC CACTGTGGGC 
GAGTCCCTGG CCTCCATCCC TCAGCCCGTG ACAGCCGTCC ACGGCCCGGG CCTGCAGGCG 
GGGGGCAGGG GCACCGTCCC GCAGCGAGTC ACCCTGTTCC GACAGATGTC CTCGGGAGCC 
ATCCCCCCGA ACCGAGGAGC GCCCCCCGCG CCCCCTCCAC CAGCAGCGGC TCTCCCCAGA 
GAGTCCTCCT CAGTCTTAAC TACAGACCCA GAGGCAGAAA AGCCACGGTT TGCTTCGAAT 
TTATGA