ADAMTS4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADAMTS4
Entrez Gene ID	488651
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif 4
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1 motif 4 Names A disintegrin and metalloproteinase with thrombospondin motifs 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_545768.5	XP_545768.3	A disintegrin and metalloproteinase with thrombospondin motifs 4
XM_545768.6	XP_545768.3	A disintegrin and metalloproteinase with thrombospondin motifs 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ADAMTS4	XP_513940.2
Canis lupus familiaris (dog)	ADAMTS4	XP_545768.3
Xenopus tropicalis (tropical clawed frog)	adamts4	XP_004919795.1
Rattus norvegicus (Norway rat)	Adamts4	NP_076449.1
Homo sapiens (human)	ADAMTS4	NP_005090.3
	ADAMTS4	XP_002808295.1
Bos taurus (cattle)	ADAMTS4	NP_858053.1
Mus musculus (house mouse)	Adamts4	NP_766433.1

Gene Ontology

The following ADAMTS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe31779	XM_545768.6 Latest version!	Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 4 (ADAMTS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCe31779	XM_545768.5	Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif, 4 (ADAMTS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADAMTS4 ( XM_545768.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545768.3 Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif, 4 (ADAMTS4), mRNA.

Price & Availability↑

CloneID	OCe31779
Clone ID Related Accession (Same CDS sequence)	XM_545768.5 , XM_545768.6
Accession Version	XM_545768.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_545768.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAAAA GCGCTGGCGC GCGGAGAGAG GCTGGGAAGG ACTCACGGGG AGACCCACAG 
ACACATAGAC ACGGGCGAGA GAGGAGGAGA GAGACAGAGA CAAAGGCACA GCGGACGAAG 
CCAGAGACAG GGCAGGCCCA GAGGAAGGCC AGACAGTGTG AGGGGCACAG GGAGGCCCGA 
GGGGCTGCAG GAGGCACGGG CAGAGGGAGA CAAAGACCCG GGAGAGGAGG GGGTGCGAGA 
GGAGTTTGGA GGAGCCAGGC CACCGGGCGC CTCTCCGCAG CCGAAGGGCC AGGTTCCCTC 
CATCTCCGCC GACGGTCCCC AGCCCCCCTG CAAACTCTGC CTCTGACCCT GGCACAAGTC 
CGAGCCCCCA GGCTGCGGAG AGGAGTTTTC CAAAGCCGGG GCGGGGAGAA CTTGGTGCGC 
AGCCGGCCTC CATCCCTCCC GGGCCGCGGC GCCAGTGCCA TGGCCCCGAC GGACCGGCGG 
CCCCGGAGGG GCTTGGCGGG GTGCTGGCTG TGGGGCGCCG GGCCCCGCCT GCCGCTGCCC 
GCGCTGCCCA CGGCCCGGCC GGCGGCGCGC CTGCTGCTGC CGCTGCTGCT GAGCGCCCTG 
CTGCCCTCGG GCCGCCCGGC CGGCCCCCTC CCCCGGGAGG AGGAGATCGT GTTTCCCGAG 
CGGCTCAACG GCAGCGTCCC GCCGGGGCTG GGCGCCCCCG CCCGGCTTCT GTGCCGCCTG 
CCCGCCTTCG GGGAGACGCT GCTGCTAGAG CTGGAGCAGG ACCCTGGCGT GCGGGTCGAG 
GGGCTGACGG TGCAGTACCT GGGCCGCGCA CCTGAGCTGC TGGGGGGCGC AGAGCCGGGC 
ACCTACCTCA CCGGCAGCAT CAACGGAGAT CCGGAGTCGG TGGCGTCCCT GCACTGGGAC 
GGGGGAGCCC TGCTAGGGGT GCTGCAGTAC CGGGGGACTG AACTGCACAT CCAGCCCCTG 
GAGGGGGGCT CCCTGAACTC CGCCGGAGGG CCCGGGGCTC ACATCCTACG CCGGAAGAGT 
CCCGCCAGTG GCCAGGGCCC CATGTGCAAC GTCAAGGCTC CCCCCGGGAA TCCCAGCCCC 
AGCCCTCGAA GAGCCAAGCG CTTTGCTTCA CTGAGTCGCT TCGTGGAGAC TCTGGTCGTG 
GCCGATGACA AGATGGCAGC ATTTCACGGC GCAGGCCTGA AGCGCTACCT GCTGACGGTC 
ATGGCCGCGG CAGCCAAGGC CTTCAAGCAC CCCAGCATCC GCAACCCCGT CAGCCTGGTG 
GTGACTCGGC TCGTGATTCT GGGGCCCGGC GAGGAAGGGC CCCAAGTGGG GCCCAGCGCC 
GCCCAGACCC TGCGCAGTTT CTGCGCCTGG CAGCGAGGAC TCAACACCCC CGAGGACTCG 
GACCCTGACC ACTTTGACAC GGCCATTCTG TTCACCCGGC AGGACCTGTG CGGGGTGTCT 
ACTTGTGACA CTCTGGGCAT GGCCGACGTG GGCACTGTGT GTGACCCGGC TCGGAGCTGT 
GCTGTTGTGG AGGATGATGG GCTTCAGTCG GCCTTCACTG CTGCTCATGA ACTGGGCCAC 
GTCTTCAACA TGCTCCACGA CAACTCAAAG TCATGTGCTG ATCTCAATGG GCCTGGGAGC 
ACCTCCCGCC ATGTCATGGC TCCTGTCATG GCTCACGTGG ACCCCGAGGA GCCCTGGTCC 
CCCTGTAGTG CCCACTTCAT CACTGAGTTC CTGGACAACG GCTATGGGCA CTGTCTCTTA 
GACAAGCCAG AGGCTCCCCT GCATCTGCCT GTGACTTTCC CCGGCAAGGA CTATGACGCT 
GATCGCCAGT GCCAGCTGAC CTTCGGGCCC GACTCACGCC ATTGTCCGCA GCTGCCGCCG 
CCCTGTGCTG CCCTCTGGTG CTCTGGCCAT CTCAATGGCC ATGCCATGTG CCAGACCAAG 
CATTCGCCCT GGGCCGACGG CACCCCCTGT GGGCCAGCAC AGGCCTGCAT GGGTGGCCGC 
TGCCTCCACG TGGACCAGCT GCAGGACTTC AATATCCCAC AGGCTGGTGG CTGGGGTCCC 
TGGGGACCGT GGGGCGACTG CTCTCGGAGC TGCGGGGGCG GCGTCCAGTT CTCCTCCCGG 
GACTGCACAA GGCCTGTCCC GCGGAACGGT GGCAAGTACT GTGAGGGCCG CCGCACCCGC 
TTCCGTTCCT GCAACACCCA GGACTGCCCG ACTGGTTCAG CACTGACCTT CCGTGAGGAG 
CAGTGTGCTG CTTACAACCA CCGCACTGAC CTCTTCAAGA GCTTCCCGGG GCCCATGGAC 
TGGGTCCCGC GCTACACCGG TGTGGCACCC CGGGACCAGT GCAAACTCAC CTGCCAGGCC 
CGGGCCCTGG GCTACTACTA TGTGCTGGAG CCGCGGGTGG TAGATGGGAC TCCCTGTTCC 
CCGGACAGCT CCTCGGTGTG TGTCCAGGGC CGCTGCATCC ATGCTGGCTG CGACCGTGTC 
ATTGGCTCCA AAAAGAAGTT TGACAAGTGC ATGGTGTGCG GTGGGGATGG TTCTAGCTGC 
AGTAAGCAGT CTGGCTCCTT CAGAAAATTC AGGTACGGAT ACAACAATGT GGTCACTATC 
CCCGCGGGGG CCACGCATAT CCTCGTCCGG CAGCAGGGGA CCCCTGGGCT CCGGAGCCTC 
TACCTGGCCC TGAAGCTCCC CGATGGCTCC TACGCCCTCA ACGGCGAATA CACGCTCATG 
CCCTCTCCCA CGGATGTGGT ACTGCCCGGG GCAGTCAGTC TCCGCTACAG CGGGGCCACT 
GCAGCCTCTG AGACGCTGTC GGGCCACGGG CCGCTGGCCC AGCCCTTAAC GCTGCAGGTC 
CTGGTCGCGG GCAACCCCCA GAACGCGCGC CTGCGGTACA GCTTCTTCGT CCCTCGGCCG 
GCCCCTGCCA CAGCCCGTCC CACGCCCCAG GACTGGCTGC AGCGCAAGGC ACAGATTCTG 
GAGATCCTCC GGCGGCGCCC CTGGGCGGGC AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545768.3
CDS	2194..5172
Translation	MLKSAGARREAGKDSRGDPQTHRHGRERRRETETKAQRTKPETGQAQRKARQCEGHREARGAAGGTGRGRQRPGRGGGARGVWRSQATGRLSAAEGPGSLHLRRRSPAPLQTLPLTLAQVRAPRLRRGVFQSRGGENLVRSRPPSLPGRGASAMAPTDRRPRRGLAGCWLWGAGPRLPLPALPTARPAARLLLPLLLSALLPSGRPAGPLPREEEIVFPERLNGSVPPGLGAPARLLCRLPAFGETLLLELEQDPGVRVEGLTVQYLGRAPELLGGAEPGTYLTGSINGDPESVASLHWDGGALLGVLQYRGTELHIQPLEGGSLNSAGGPGAHILRRKSPASGQGPMCNVKAPPGNPSPSPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGPGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQDLCGVSTCDTLGMADVGTVCDPARSCAVVEDDGLQSAFTAAHELGHVFNMLHDNSKSCADLNGPGSTSRHVMAPVMAHVDPEEPWSPCSAHFITEFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSRHCPQLPPPCAALWCSGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLHVDQLQDFNIPQAGGWGPWGPWGDCSRSCGGGVQFSSRDCTRPVPRNGGKYCEGRRTRFRSCNTQDCPTGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPRDQCKLTCQARALGYYYVLEPRVVDGTPCSPDSSSVCVQGRCIHAGCDRVIGSKKKFDKCMVCGGDGSSCSKQSGSFRKFRYGYNNVVTIPAGATHILVRQQGTPGLRSLYLALKLPDGSYALNGEYTLMPSPTDVVLPGAVSLRYSGATAASETLSGHGPLAQPLTLQVLVAGNPQNARLRYSFFVPRPAPATARPTPQDWLQRKAQILEILRRRPWAGRK

Target ORF information:

RefSeq Version	XM_545768.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif, 4 (ADAMTS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545768.5

ORF Insert Sequence:

ATGCTTAAAA GCGCTGGCGC GCGGAGAGAG GCTGGGAAGG ACTCACGGGG AGACCCACAG 
ACACATAGAC ACGGGCGAGA GAGGAGGAGA GAGACAGAGA CAAAGGCACA GCGGACGAAG 
CCAGAGACAG GGCAGGCCCA GAGGAAGGCC AGACAGTGTG AGGGGCACAG GGAGGCCCGA 
GGGGCTGCAG GAGGCACGGG CAGAGGGAGA CAAAGACCCG GGAGAGGAGG GGGTGCGAGA 
GGAGTTTGGA GGAGCCAGGC CACCGGGCGC CTCTCCGCAG CCGAAGGGCC AGGTTCCCTC 
CATCTCCGCC GACGGTCCCC AGCCCCCCTG CAAACTCTGC CTCTGACCCT GGCACAAGTC 
CGAGCCCCCA GGCTGCGGAG AGGAGTTTTC CAAAGCCGGG GCGGGGAGAA CTTGGTGCGC 
AGCCGGCCTC CATCCCTCCC GGGCCGCGGC GCCAGTGCCA TGGCCCCGAC GGACCGGCGG 
CCCCGGAGGG GCTTGGCGGG GTGCTGGCTG TGGGGCGCCG GGCCCCGCCT GCCGCTGCCC 
GCGCTGCCCA CGGCCCGGCC GGCGGCGCGC CTGCTGCTGC CGCTGCTGCT GAGCGCCCTG 
CTGCCCTCGG GCCGCCCGGC CGGCCCCCTC CCCCGGGAGG AGGAGATCGT GTTTCCCGAG 
CGGCTCAACG GCAGCGTCCC GCCGGGGCTG GGCGCCCCCG CCCGGCTTCT GTGCCGCCTG 
CCCGCCTTCG GGGAGACGCT GCTGCTAGAG CTGGAGCAGG ACCCTGGCGT GCGGGTCGAG 
GGGCTGACGG TGCAGTACCT GGGCCGCGCA CCTGAGCTGC TGGGGGGCGC AGAGCCGGGC 
ACCTACCTCA CCGGCAGCAT CAACGGAGAT CCGGAGTCGG TGGCGTCCCT GCACTGGGAC 
GGGGGAGCCC TGCTAGGGGT GCTGCAGTAC CGGGGGACTG AACTGCACAT CCAGCCCCTG 
GAGGGGGGCT CCCTGAACTC CGCCGGAGGG CCCGGGGCTC ACATCCTACG CCGGAAGAGT 
CCCGCCAGTG GCCAGGGCCC CATGTGCAAC GTCAAGGCTC CCCCCGGGAA TCCCAGCCCC 
AGCCCTCGAA GAGCCAAGCG CTTTGCTTCA CTGAGTCGCT TCGTGGAGAC TCTGGTCGTG 
GCCGATGACA AGATGGCAGC ATTTCACGGC GCAGGCCTGA AGCGCTACCT GCTGACGGTC 
ATGGCCGCGG CAGCCAAGGC CTTCAAGCAC CCCAGCATCC GCAACCCCGT CAGCCTGGTG 
GTGACTCGGC TCGTGATTCT GGGGCCCGGC GAGGAAGGGC CCCAAGTGGG GCCCAGCGCC 
GCCCAGACCC TGCGCAGTTT CTGCGCCTGG CAGCGAGGAC TCAACACCCC CGAGGACTCG 
GACCCTGACC ACTTTGACAC GGCCATTCTG TTCACCCGGC AGGACCTGTG CGGGGTGTCT 
ACTTGTGACA CTCTGGGCAT GGCCGACGTG GGCACTGTGT GTGACCCGGC TCGGAGCTGT 
GCTGTTGTGG AGGATGATGG GCTTCAGTCG GCCTTCACTG CTGCTCATGA ACTGGGCCAC 
GTCTTCAACA TGCTCCACGA CAACTCAAAG TCATGTGCTG ATCTCAATGG GCCTGGGAGC 
ACCTCCCGCC ATGTCATGGC TCCTGTCATG GCTCACGTGG ACCCCGAGGA GCCCTGGTCC 
CCCTGTAGTG CCCACTTCAT CACTGAGTTC CTGGACAACG GCTATGGGCA CTGTCTCTTA 
GACAAGCCAG AGGCTCCCCT GCATCTGCCT GTGACTTTCC CCGGCAAGGA CTATGACGCT 
GATCGCCAGT GCCAGCTGAC CTTCGGGCCC GACTCACGCC ATTGTCCGCA GCTGCCGCCG 
CCCTGTGCTG CCCTCTGGTG CTCTGGCCAT CTCAATGGCC ATGCCATGTG CCAGACCAAG 
CATTCGCCCT GGGCCGACGG CACCCCCTGT GGGCCAGCAC AGGCCTGCAT GGGTGGCCGC 
TGCCTCCACG TGGACCAGCT GCAGGACTTC AATATCCCAC AGGCTGGTGG CTGGGGTCCC 
TGGGGACCGT GGGGCGACTG CTCTCGGAGC TGCGGGGGCG GCGTCCAGTT CTCCTCCCGG 
GACTGCACAA GGCCTGTCCC GCGGAACGGT GGCAAGTACT GTGAGGGCCG CCGCACCCGC 
TTCCGTTCCT GCAACACCCA GGACTGCCCG ACTGGTTCAG CACTGACCTT CCGTGAGGAG 
CAGTGTGCTG CTTACAACCA CCGCACTGAC CTCTTCAAGA GCTTCCCGGG GCCCATGGAC 
TGGGTCCCGC GCTACACCGG TGTGGCACCC CGGGACCAGT GCAAACTCAC CTGCCAGGCC 
CGGGCCCTGG GCTACTACTA TGTGCTGGAG CCGCGGGTGG TAGATGGGAC TCCCTGTTCC 
CCGGACAGCT CCTCGGTGTG TGTCCAGGGC CGCTGCATCC ATGCTGGCTG CGACCGTGTC 
ATTGGCTCCA AAAAGAAGTT TGACAAGTGC ATGGTGTGCG GTGGGGATGG TTCTAGCTGC 
AGTAAGCAGT CTGGCTCCTT CAGAAAATTC AGGTACGGAT ACAACAATGT GGTCACTATC 
CCCGCGGGGG CCACGCATAT CCTCGTCCGG CAGCAGGGGA CCCCTGGGCT CCGGAGCCTC 
TACCTGGCCC TGAAGCTCCC CGATGGCTCC TACGCCCTCA ACGGCGAATA CACGCTCATG 
CCCTCTCCCA CGGATGTGGT ACTGCCCGGG GCAGTCAGTC TCCGCTACAG CGGGGCCACT 
GCAGCCTCTG AGACGCTGTC GGGCCACGGG CCGCTGGCCC AGCCCTTAAC GCTGCAGGTC 
CTGGTCGCGG GCAACCCCCA GAACGCGCGC CTGCGGTACA GCTTCTTCGT CCCTCGGCCG 
GCCCCTGCCA CAGCCCGTCC CACGCCCCAG GACTGGCTGC AGCGCAAGGC ACAGATTCTG 
GAGATCCTCC GGCGGCGCCC CTGGGCGGGC AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS4 ( XM_545768.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545768.3 Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 4 (ADAMTS4), mRNA.

Price & Availability↑

CloneID	OCe31779
Clone ID Related Accession (Same CDS sequence)	XM_545768.5 , XM_545768.6
Accession Version	XM_545768.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545768.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAAAA GCGCTGGCGC GCGGAGAGAG GCTGGGAAGG ACTCACGGGG AGACCCACAG 
ACACATAGAC ACGGGCGAGA GAGGAGGAGA GAGACAGAGA CAAAGGCACA GCGGACGAAG 
CCAGAGACAG GGCAGGCCCA GAGGAAGGCC AGACAGTGTG AGGGGCACAG GGAGGCCCGA 
GGGGCTGCAG GAGGCACGGG CAGAGGGAGA CAAAGACCCG GGAGAGGAGG GGGTGCGAGA 
GGAGTTTGGA GGAGCCAGGC CACCGGGCGC CTCTCCGCAG CCGAAGGGCC AGGTTCCCTC 
CATCTCCGCC GACGGTCCCC AGCCCCCCTG CAAACTCTGC CTCTGACCCT GGCACAAGTC 
CGAGCCCCCA GGCTGCGGAG AGGAGTTTTC CAAAGCCGGG GCGGGGAGAA CTTGGTGCGC 
AGCCGGCCTC CATCCCTCCC GGGCCGCGGC GCCAGTGCCA TGGCCCCGAC GGACCGGCGG 
CCCCGGAGGG GCTTGGCGGG GTGCTGGCTG TGGGGCGCCG GGCCCCGCCT GCCGCTGCCC 
GCGCTGCCCA CGGCCCGGCC GGCGGCGCGC CTGCTGCTGC CGCTGCTGCT GAGCGCCCTG 
CTGCCCTCGG GCCGCCCGGC CGGCCCCCTC CCCCGGGAGG AGGAGATCGT GTTTCCCGAG 
CGGCTCAACG GCAGCGTCCC GCCGGGGCTG GGCGCCCCCG CCCGGCTTCT GTGCCGCCTG 
CCCGCCTTCG GGGAGACGCT GCTGCTAGAG CTGGAGCAGG ACCCTGGCGT GCGGGTCGAG 
GGGCTGACGG TGCAGTACCT GGGCCGCGCA CCTGAGCTGC TGGGGGGCGC AGAGCCGGGC 
ACCTACCTCA CCGGCAGCAT CAACGGAGAT CCGGAGTCGG TGGCGTCCCT GCACTGGGAC 
GGGGGAGCCC TGCTAGGGGT GCTGCAGTAC CGGGGGACTG AACTGCACAT CCAGCCCCTG 
GAGGGGGGCT CCCTGAACTC CGCCGGAGGG CCCGGGGCTC ACATCCTACG CCGGAAGAGT 
CCCGCCAGTG GCCAGGGCCC CATGTGCAAC GTCAAGGCTC CCCCCGGGAA TCCCAGCCCC 
AGCCCTCGAA GAGCCAAGCG CTTTGCTTCA CTGAGTCGCT TCGTGGAGAC TCTGGTCGTG 
GCCGATGACA AGATGGCAGC ATTTCACGGC GCAGGCCTGA AGCGCTACCT GCTGACGGTC 
ATGGCCGCGG CAGCCAAGGC CTTCAAGCAC CCCAGCATCC GCAACCCCGT CAGCCTGGTG 
GTGACTCGGC TCGTGATTCT GGGGCCCGGC GAGGAAGGGC CCCAAGTGGG GCCCAGCGCC 
GCCCAGACCC TGCGCAGTTT CTGCGCCTGG CAGCGAGGAC TCAACACCCC CGAGGACTCG 
GACCCTGACC ACTTTGACAC GGCCATTCTG TTCACCCGGC AGGACCTGTG CGGGGTGTCT 
ACTTGTGACA CTCTGGGCAT GGCCGACGTG GGCACTGTGT GTGACCCGGC TCGGAGCTGT 
GCTGTTGTGG AGGATGATGG GCTTCAGTCG GCCTTCACTG CTGCTCATGA ACTGGGCCAC 
GTCTTCAACA TGCTCCACGA CAACTCAAAG TCATGTGCTG ATCTCAATGG GCCTGGGAGC 
ACCTCCCGCC ATGTCATGGC TCCTGTCATG GCTCACGTGG ACCCCGAGGA GCCCTGGTCC 
CCCTGTAGTG CCCACTTCAT CACTGAGTTC CTGGACAACG GCTATGGGCA CTGTCTCTTA 
GACAAGCCAG AGGCTCCCCT GCATCTGCCT GTGACTTTCC CCGGCAAGGA CTATGACGCT 
GATCGCCAGT GCCAGCTGAC CTTCGGGCCC GACTCACGCC ATTGTCCGCA GCTGCCGCCG 
CCCTGTGCTG CCCTCTGGTG CTCTGGCCAT CTCAATGGCC ATGCCATGTG CCAGACCAAG 
CATTCGCCCT GGGCCGACGG CACCCCCTGT GGGCCAGCAC AGGCCTGCAT GGGTGGCCGC 
TGCCTCCACG TGGACCAGCT GCAGGACTTC AATATCCCAC AGGCTGGTGG CTGGGGTCCC 
TGGGGACCGT GGGGCGACTG CTCTCGGAGC TGCGGGGGCG GCGTCCAGTT CTCCTCCCGG 
GACTGCACAA GGCCTGTCCC GCGGAACGGT GGCAAGTACT GTGAGGGCCG CCGCACCCGC 
TTCCGTTCCT GCAACACCCA GGACTGCCCG ACTGGTTCAG CACTGACCTT CCGTGAGGAG 
CAGTGTGCTG CTTACAACCA CCGCACTGAC CTCTTCAAGA GCTTCCCGGG GCCCATGGAC 
TGGGTCCCGC GCTACACCGG TGTGGCACCC CGGGACCAGT GCAAACTCAC CTGCCAGGCC 
CGGGCCCTGG GCTACTACTA TGTGCTGGAG CCGCGGGTGG TAGATGGGAC TCCCTGTTCC 
CCGGACAGCT CCTCGGTGTG TGTCCAGGGC CGCTGCATCC ATGCTGGCTG CGACCGTGTC 
ATTGGCTCCA AAAAGAAGTT TGACAAGTGC ATGGTGTGCG GTGGGGATGG TTCTAGCTGC 
AGTAAGCAGT CTGGCTCCTT CAGAAAATTC AGGTACGGAT ACAACAATGT GGTCACTATC 
CCCGCGGGGG CCACGCATAT CCTCGTCCGG CAGCAGGGGA CCCCTGGGCT CCGGAGCCTC 
TACCTGGCCC TGAAGCTCCC CGATGGCTCC TACGCCCTCA ACGGCGAATA CACGCTCATG 
CCCTCTCCCA CGGATGTGGT ACTGCCCGGG GCAGTCAGTC TCCGCTACAG CGGGGCCACT 
GCAGCCTCTG AGACGCTGTC GGGCCACGGG CCGCTGGCCC AGCCCTTAAC GCTGCAGGTC 
CTGGTCGCGG GCAACCCCCA GAACGCGCGC CTGCGGTACA GCTTCTTCGT CCCTCGGCCG 
GCCCCTGCCA CAGCCCGTCC CACGCCCCAG GACTGGCTGC AGCGCAAGGC ACAGATTCTG 
GAGATCCTCC GGCGGCGCCC CTGGGCGGGC AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545768.3
CDS	776..3754
Translation	MLKSAGARREAGKDSRGDPQTHRHGRERRRETETKAQRTKPETGQAQRKARQCEGHREARGAAGGTGRGRQRPGRGGGARGVWRSQATGRLSAAEGPGSLHLRRRSPAPLQTLPLTLAQVRAPRLRRGVFQSRGGENLVRSRPPSLPGRGASAMAPTDRRPRRGLAGCWLWGAGPRLPLPALPTARPAARLLLPLLLSALLPSGRPAGPLPREEEIVFPERLNGSVPPGLGAPARLLCRLPAFGETLLLELEQDPGVRVEGLTVQYLGRAPELLGGAEPGTYLTGSINGDPESVASLHWDGGALLGVLQYRGTELHIQPLEGGSLNSAGGPGAHILRRKSPASGQGPMCNVKAPPGNPSPSPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGPGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQDLCGVSTCDTLGMADVGTVCDPARSCAVVEDDGLQSAFTAAHELGHVFNMLHDNSKSCADLNGPGSTSRHVMAPVMAHVDPEEPWSPCSAHFITEFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSRHCPQLPPPCAALWCSGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLHVDQLQDFNIPQAGGWGPWGPWGDCSRSCGGGVQFSSRDCTRPVPRNGGKYCEGRRTRFRSCNTQDCPTGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPRDQCKLTCQARALGYYYVLEPRVVDGTPCSPDSSSVCVQGRCIHAGCDRVIGSKKKFDKCMVCGGDGSSCSKQSGSFRKFRYGYNNVVTIPAGATHILVRQQGTPGLRSLYLALKLPDGSYALNGEYTLMPSPTDVVLPGAVSLRYSGATAASETLSGHGPLAQPLTLQVLVAGNPQNARLRYSFFVPRPAPATARPTPQDWLQRKAQILEILRRRPWAGRK

Target ORF information:

RefSeq Version	XM_545768.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 4 (ADAMTS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545768.6

ORF Insert Sequence:

ATGCTTAAAA GCGCTGGCGC GCGGAGAGAG GCTGGGAAGG ACTCACGGGG AGACCCACAG 
ACACATAGAC ACGGGCGAGA GAGGAGGAGA GAGACAGAGA CAAAGGCACA GCGGACGAAG 
CCAGAGACAG GGCAGGCCCA GAGGAAGGCC AGACAGTGTG AGGGGCACAG GGAGGCCCGA 
GGGGCTGCAG GAGGCACGGG CAGAGGGAGA CAAAGACCCG GGAGAGGAGG GGGTGCGAGA 
GGAGTTTGGA GGAGCCAGGC CACCGGGCGC CTCTCCGCAG CCGAAGGGCC AGGTTCCCTC 
CATCTCCGCC GACGGTCCCC AGCCCCCCTG CAAACTCTGC CTCTGACCCT GGCACAAGTC 
CGAGCCCCCA GGCTGCGGAG AGGAGTTTTC CAAAGCCGGG GCGGGGAGAA CTTGGTGCGC 
AGCCGGCCTC CATCCCTCCC GGGCCGCGGC GCCAGTGCCA TGGCCCCGAC GGACCGGCGG 
CCCCGGAGGG GCTTGGCGGG GTGCTGGCTG TGGGGCGCCG GGCCCCGCCT GCCGCTGCCC 
GCGCTGCCCA CGGCCCGGCC GGCGGCGCGC CTGCTGCTGC CGCTGCTGCT GAGCGCCCTG 
CTGCCCTCGG GCCGCCCGGC CGGCCCCCTC CCCCGGGAGG AGGAGATCGT GTTTCCCGAG 
CGGCTCAACG GCAGCGTCCC GCCGGGGCTG GGCGCCCCCG CCCGGCTTCT GTGCCGCCTG 
CCCGCCTTCG GGGAGACGCT GCTGCTAGAG CTGGAGCAGG ACCCTGGCGT GCGGGTCGAG 
GGGCTGACGG TGCAGTACCT GGGCCGCGCA CCTGAGCTGC TGGGGGGCGC AGAGCCGGGC 
ACCTACCTCA CCGGCAGCAT CAACGGAGAT CCGGAGTCGG TGGCGTCCCT GCACTGGGAC 
GGGGGAGCCC TGCTAGGGGT GCTGCAGTAC CGGGGGACTG AACTGCACAT CCAGCCCCTG 
GAGGGGGGCT CCCTGAACTC CGCCGGAGGG CCCGGGGCTC ACATCCTACG CCGGAAGAGT 
CCCGCCAGTG GCCAGGGCCC CATGTGCAAC GTCAAGGCTC CCCCCGGGAA TCCCAGCCCC 
AGCCCTCGAA GAGCCAAGCG CTTTGCTTCA CTGAGTCGCT TCGTGGAGAC TCTGGTCGTG 
GCCGATGACA AGATGGCAGC ATTTCACGGC GCAGGCCTGA AGCGCTACCT GCTGACGGTC 
ATGGCCGCGG CAGCCAAGGC CTTCAAGCAC CCCAGCATCC GCAACCCCGT CAGCCTGGTG 
GTGACTCGGC TCGTGATTCT GGGGCCCGGC GAGGAAGGGC CCCAAGTGGG GCCCAGCGCC 
GCCCAGACCC TGCGCAGTTT CTGCGCCTGG CAGCGAGGAC TCAACACCCC CGAGGACTCG 
GACCCTGACC ACTTTGACAC GGCCATTCTG TTCACCCGGC AGGACCTGTG CGGGGTGTCT 
ACTTGTGACA CTCTGGGCAT GGCCGACGTG GGCACTGTGT GTGACCCGGC TCGGAGCTGT 
GCTGTTGTGG AGGATGATGG GCTTCAGTCG GCCTTCACTG CTGCTCATGA ACTGGGCCAC 
GTCTTCAACA TGCTCCACGA CAACTCAAAG TCATGTGCTG ATCTCAATGG GCCTGGGAGC 
ACCTCCCGCC ATGTCATGGC TCCTGTCATG GCTCACGTGG ACCCCGAGGA GCCCTGGTCC 
CCCTGTAGTG CCCACTTCAT CACTGAGTTC CTGGACAACG GCTATGGGCA CTGTCTCTTA 
GACAAGCCAG AGGCTCCCCT GCATCTGCCT GTGACTTTCC CCGGCAAGGA CTATGACGCT 
GATCGCCAGT GCCAGCTGAC CTTCGGGCCC GACTCACGCC ATTGTCCGCA GCTGCCGCCG 
CCCTGTGCTG CCCTCTGGTG CTCTGGCCAT CTCAATGGCC ATGCCATGTG CCAGACCAAG 
CATTCGCCCT GGGCCGACGG CACCCCCTGT GGGCCAGCAC AGGCCTGCAT GGGTGGCCGC 
TGCCTCCACG TGGACCAGCT GCAGGACTTC AATATCCCAC AGGCTGGTGG CTGGGGTCCC 
TGGGGACCGT GGGGCGACTG CTCTCGGAGC TGCGGGGGCG GCGTCCAGTT CTCCTCCCGG 
GACTGCACAA GGCCTGTCCC GCGGAACGGT GGCAAGTACT GTGAGGGCCG CCGCACCCGC 
TTCCGTTCCT GCAACACCCA GGACTGCCCG ACTGGTTCAG CACTGACCTT CCGTGAGGAG 
CAGTGTGCTG CTTACAACCA CCGCACTGAC CTCTTCAAGA GCTTCCCGGG GCCCATGGAC 
TGGGTCCCGC GCTACACCGG TGTGGCACCC CGGGACCAGT GCAAACTCAC CTGCCAGGCC 
CGGGCCCTGG GCTACTACTA TGTGCTGGAG CCGCGGGTGG TAGATGGGAC TCCCTGTTCC 
CCGGACAGCT CCTCGGTGTG TGTCCAGGGC CGCTGCATCC ATGCTGGCTG CGACCGTGTC 
ATTGGCTCCA AAAAGAAGTT TGACAAGTGC ATGGTGTGCG GTGGGGATGG TTCTAGCTGC 
AGTAAGCAGT CTGGCTCCTT CAGAAAATTC AGGTACGGAT ACAACAATGT GGTCACTATC 
CCCGCGGGGG CCACGCATAT CCTCGTCCGG CAGCAGGGGA CCCCTGGGCT CCGGAGCCTC 
TACCTGGCCC TGAAGCTCCC CGATGGCTCC TACGCCCTCA ACGGCGAATA CACGCTCATG 
CCCTCTCCCA CGGATGTGGT ACTGCCCGGG GCAGTCAGTC TCCGCTACAG CGGGGCCACT 
GCAGCCTCTG AGACGCTGTC GGGCCACGGG CCGCTGGCCC AGCCCTTAAC GCTGCAGGTC 
CTGGTCGCGG GCAACCCCCA GAACGCGCGC CTGCGGTACA GCTTCTTCGT CCCTCGGCCG 
GCCCCTGCCA CAGCCCGTCC CACGCCCCAG GACTGGCTGC AGCGCAAGGC ACAGATTCTG 
GAGATCCTCC GGCGGCGCCC CTGGGCGGGC AGGAAATAA