KIF1A gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022409572.1	XP_022265280.1	kinesin-like protein KIF1A isoform X9
XM_022409573.1	XP_022265281.1	kinesin-like protein KIF1A isoform X10
XM_014107687.1	XP_013963162.1	kinesin-like protein KIF1A isoform X9
XM_005635953.2	XP_005636010.1	kinesin-like protein KIF1A isoform X12
XM_005635951.2	XP_005636008.1	kinesin-like protein KIF1A isoform X10
XM_014107683.2	XP_013963158.1	kinesin-like protein KIF1A isoform X5
XM_022409571.1	XP_022265279.1	kinesin-like protein KIF1A isoform X7
XM_005635949.2	XP_005636006.1	kinesin-like protein KIF1A isoform X6
XM_005635952.2	XP_005636009.1	kinesin-like protein KIF1A isoform X11
XM_014107688.1	XP_013963163.1	kinesin-like protein KIF1A isoform X13
XM_014107682.1	XP_013963157.1	kinesin-like protein KIF1A isoform X3
XM_014107686.1	XP_013963161.1	kinesin-like protein KIF1A isoform X8
XM_022409570.1	XP_022265278.1	kinesin-like protein KIF1A isoform X6
XM_014107681.1	XP_013963156.1	kinesin-like protein KIF1A isoform X2
XM_022409569.1	XP_022265277.1	kinesin-like protein KIF1A isoform X3
XM_005635949.3	XP_005636006.1	kinesin-like protein KIF1A isoform X8
XM_022409576.1	XP_022265284.1	kinesin-like protein KIF1A isoform X16
XM_014107688.2	XP_013963163.1	kinesin-like protein KIF1A isoform X17
NM_001168473.2	NP_001161945.2	kinesin-like protein KIF1A
XM_014107683.1	XP_013963158.1	kinesin-like protein KIF1A isoform X4
XM_014107684.1	XP_013963159.1	kinesin-like protein KIF1A isoform X5
XM_014107685.1	XP_013963160.1	kinesin-like protein KIF1A isoform X7
XM_014107680.1	XP_013963155.1	kinesin-like protein KIF1A isoform X1
XM_022409568.1	XP_022265276.1	kinesin-like protein KIF1A isoform X1
XM_014107680.2	XP_013963155.1	kinesin-like protein KIF1A isoform X2
XM_014107681.2	XP_013963156.1	kinesin-like protein KIF1A isoform X4
XM_022409574.1	XP_022265282.1	kinesin-like protein KIF1A isoform X11
XM_022409575.1	XP_022265283.1	kinesin-like protein KIF1A isoform X12
XM_005635951.3	XP_005636008.1	kinesin-like protein KIF1A isoform X13
XM_005635952.3	XP_005636009.1	kinesin-like protein KIF1A isoform X14
XM_005635953.3	XP_005636010.1	kinesin-like protein KIF1A isoform X15

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe297015	XM_022409573.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe297016	XM_022409574.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe297017	XM_022409575.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43171	XM_014107687.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe25957	NM_001168473.2 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25955	XM_005635951.3 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25956	XM_005635952.3 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25957	XM_005635953.3 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25954	XM_005635949.3 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297010	XM_022409568.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297011	XM_022409569.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297012	XM_022409570.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297013	XM_022409571.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297014	XM_022409572.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe297018	XM_022409576.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43164	XM_014107680.2 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43165	XM_014107681.2 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43166	XM_014107682.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43167	XM_014107683.2 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43168	XM_014107684.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43169	XM_014107685.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43170	XM_014107686.1 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43172	XM_014107688.2 Latest version!	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe25954	XM_005635949.2	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43172	XM_014107688.1	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43167	XM_014107683.1	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43164	XM_014107680.1	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe43165	XM_014107681.1	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe25955	XM_005635951.2	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25956	XM_005635952.2	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe25957	XM_005635953.2	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

KIF1A ( XM_022409573.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265281.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe297015
Clone ID Related Accession (Same CDS sequence)	XM_022409573.1
Accession Version	XM_022409573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCATTCGGAA ATGACACTAG GACCTTCTAC CAATTCGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCTC TTCTGCTCAA CCGGGTCACC CCGTACCGAG AGAAGATCTA CATGACTCTC 
TCAGCTTACA TTGAGATGGA GAACTGCACT CAGCCGGCCG TCATCACCAA GGACTTCTGC 
ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG CCCGCTTCCC GCTCCATCCG CAACCTGTTT 
GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTCAGC 
CTGTGCCATG TGGCCGACGC CGGCAGCCCA GGGATGCAGC GGCGGCGGCG GCGCGTGCTG 
GATACATCCG TGGCCTACGT GCGGGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGGAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT 
GAGAAGACCA GACACTACCT TCTGCTGCGG GAGAAGCTGG AGACCACCCA GCGGCCCGGC 
CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG GACTTGGGGT CCCATGGCTC CTCCAGCCCC 
TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG TCGCCTCTGG AGACTCCCAA TGAGAGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGCCTC CTCACGCACA CGTTCAACCG AGAGTACACC 
CACAGCCACG TCTGCATCAG TGCCAGCGAG AGCAAGCTCT CCGAGATGTC TGTCACCCTG 
CTTCGGGACC CATCCATGTC CCCGCTGGGG GCGGCCACCC TCACCCCCTC CTCCACCTGC 
CCCTCTCTGG TCGAAGGGCG GTACGGAGCC GCTGAGCTGA GGACCCCCCA GCCCTGCTCC 
CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC 
CCTGCTCGGG CTGCGGAGGC CGACAAGGAG CCCCAGCGCC TGCTGGTTCC CGACATCCAG 
GAGATCCGGG TCAGCCCCAT CGTGTCTAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTACGGCGGC CCTACGCCTA CCTGTACAAC 
AGCGACAAGG ACTCCGTGGA GAGGTTCGTG CTCAACCTGT CCACCGCGCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCCAT GCTCAAGACG CCCAACACGT TCGCCGTGTG CACGGAGCAC 
CGCGGCATCC TGCTGCAAGC CAACAGCGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCGCTCC TGGCCGGGAC CATACGGTCC AAGCTCTCCA GAAGGCGGTC TGCCCAGATG 
AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265281.1
CDS	207..5435
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409573.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409573.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCATTCGGAA ATGACACTAG GACCTTCTAC CAATTCGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCTC TTCTGCTCAA CCGGGTCACC CCGTACCGAG AGAAGATCTA CATGACTCTC 
TCAGCTTACA TTGAGATGGA GAACTGCACT CAGCCGGCCG TCATCACCAA GGACTTCTGC 
ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG CCCGCTTCCC GCTCCATCCG CAACCTGTTT 
GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTCAGC 
CTGTGCCATG TGGCCGACGC CGGCAGCCCA GGGATGCAGC GGCGGCGGCG GCGCGTGCTG 
GATACATCCG TGGCCTACGT GCGGGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGGAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT 
GAGAAGACCA GACACTACCT TCTGCTGCGG GAGAAGCTGG AGACCACCCA GCGGCCCGGC 
CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG GACTTGGGGT CCCATGGCTC CTCCAGCCCC 
TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG TCGCCTCTGG AGACTCCCAA TGAGAGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGCCTC CTCACGCACA CGTTCAACCG AGAGTACACC 
CACAGCCACG TCTGCATCAG TGCCAGCGAG AGCAAGCTCT CCGAGATGTC TGTCACCCTG 
CTTCGGGACC CATCCATGTC CCCGCTGGGG GCGGCCACCC TCACCCCCTC CTCCACCTGC 
CCCTCTCTGG TCGAAGGGCG GTACGGAGCC GCTGAGCTGA GGACCCCCCA GCCCTGCTCC 
CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC 
CCTGCTCGGG CTGCGGAGGC CGACAAGGAG CCCCAGCGCC TGCTGGTTCC CGACATCCAG 
GAGATCCGGG TCAGCCCCAT CGTGTCTAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTACGGCGGC CCTACGCCTA CCTGTACAAC 
AGCGACAAGG ACTCCGTGGA GAGGTTCGTG CTCAACCTGT CCACCGCGCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCCAT GCTCAAGACG CCCAACACGT TCGCCGTGTG CACGGAGCAC 
CGCGGCATCC TGCTGCAAGC CAACAGCGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCGCTCC TGGCCGGGAC CATACGGTCC AAGCTCTCCA GAAGGCGGTC TGCCCAGATG 
AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409574.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265282.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

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CloneID	OCe297016
Clone ID Related Accession (Same CDS sequence)	XM_022409574.1
Accession Version	XM_022409574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG ACCTTCTACC AATTCGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCTCT TCTGCTCAAC CGGGTCACCC CGTACCGAGA GAAGATCTAC 
ATGACTCTCT CAGCTTACAT TGAGATGGAG AACTGCACTC AGCCGGCCGT CATCACCAAG 
GACTTCTGCA TGGTCTTCTA CTCTCGGGAC GCCAAGCTGC CCGCTTCCCG CTCCATCCGC 
AACCTGTTTG GCAGCGGGAG CCTCCGGGCC TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTCAGCC TGTGCCATGT GGCCGACGCC GGCAGCCCAG GGATGCAGCG GCGGCGGCGG 
CGCGTGCTGG ATACATCCGT GGCCTACGTG CGGGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGGAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTTG AGAAGACCAG ACACTACCTT CTGCTGCGGG AGAAGCTGGA GACCACCCAG 
CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC TCCAGCGAGG ACTTGGGGTC CCATGGCTCC 
TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG GGCCGCCCGT CGCCTCTGGA GACTCCCAAT 
GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGCCTCC TCACGCACAC GTTCAACCGA 
GAGTACACCC ACAGCCACGT CTGCATCAGT GCCAGCGAGA GCAAGCTCTC CGAGATGTCT 
GTCACCCTGC TTCGGGACCC ATCCATGTCC CCGCTGGGGG CGGCCACCCT CACCCCCTCC 
TCCACCTGCC CCTCTCTGGT CGAAGGGCGG TACGGAGCCG CTGAGCTGAG GACCCCCCAG 
CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG 
CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC GACAAGGAGC CCCAGCGCCT GCTGGTTCCC 
GACATCCAGG AGATCCGGGT CAGCCCCATC GTGTCTAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TACGGCGGCC CTACGCCTAC 
CTGTACAACA GCGACAAGGA CTCCGTGGAG AGGTTCGTGC TCAACCTGTC CACCGCGCAG 
GTGGAGTACA GCGAGGACCA GCAGGCCATG CTCAAGACGC CCAACACGTT CGCCGTGTGC 
ACGGAGCACC GCGGCATCCT GCTGCAAGCC AACAGCGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCGCTCCT GGCCGGGACC ATACGGTCCA AGCTCTCCAG AAGGCGGTCT 
GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265282.1
CDS	207..5384
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409574.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409574.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG ACCTTCTACC AATTCGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCTCT TCTGCTCAAC CGGGTCACCC CGTACCGAGA GAAGATCTAC 
ATGACTCTCT CAGCTTACAT TGAGATGGAG AACTGCACTC AGCCGGCCGT CATCACCAAG 
GACTTCTGCA TGGTCTTCTA CTCTCGGGAC GCCAAGCTGC CCGCTTCCCG CTCCATCCGC 
AACCTGTTTG GCAGCGGGAG CCTCCGGGCC TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTCAGCC TGTGCCATGT GGCCGACGCC GGCAGCCCAG GGATGCAGCG GCGGCGGCGG 
CGCGTGCTGG ATACATCCGT GGCCTACGTG CGGGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGGAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTTG AGAAGACCAG ACACTACCTT CTGCTGCGGG AGAAGCTGGA GACCACCCAG 
CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC TCCAGCGAGG ACTTGGGGTC CCATGGCTCC 
TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG GGCCGCCCGT CGCCTCTGGA GACTCCCAAT 
GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGCCTCC TCACGCACAC GTTCAACCGA 
GAGTACACCC ACAGCCACGT CTGCATCAGT GCCAGCGAGA GCAAGCTCTC CGAGATGTCT 
GTCACCCTGC TTCGGGACCC ATCCATGTCC CCGCTGGGGG CGGCCACCCT CACCCCCTCC 
TCCACCTGCC CCTCTCTGGT CGAAGGGCGG TACGGAGCCG CTGAGCTGAG GACCCCCCAG 
CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG 
CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC GACAAGGAGC CCCAGCGCCT GCTGGTTCCC 
GACATCCAGG AGATCCGGGT CAGCCCCATC GTGTCTAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TACGGCGGCC CTACGCCTAC 
CTGTACAACA GCGACAAGGA CTCCGTGGAG AGGTTCGTGC TCAACCTGTC CACCGCGCAG 
GTGGAGTACA GCGAGGACCA GCAGGCCATG CTCAAGACGC CCAACACGTT CGCCGTGTGC 
ACGGAGCACC GCGGCATCCT GCTGCAAGCC AACAGCGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCGCTCCT GGCCGGGACC ATACGGTCCA AGCTCTCCAG AAGGCGGTCT 
GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409575.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265283.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

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CloneID	OCe297017
Clone ID Related Accession (Same CDS sequence)	XM_022409575.1
Accession Version	XM_022409575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGGA CCTTCTACCA ATTCGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCTCTT CTGCTCAACC GGGTCACCCC GTACCGAGAG 
AAGATCTACA TGACTCTCTC AGCTTACATT GAGATGGAGA ACTGCACTCA GCCGGCCGTC 
ATCACCAAGG ACTTCTGCAT GGTCTTCTAC TCTCGGGACG CCAAGCTGCC CGCTTCCCGC 
TCCATCCGCA ACCTGTTTGG CAGCGGGAGC CTCCGGGCCT CGGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTCAGCCT GTGCCATGTG GCCGACGCCG GCAGCCCAGG GATGCAGCGG 
CGGCGGCGGC GCGTGCTGGA TACATCCGTG GCCTACGTGC GGGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGGAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTTGA GAAGACCAGA CACTACCTTC TGCTGCGGGA GAAGCTGGAG 
ACCACCCAGC GGCCCGGCCC TGAGGCGCCG TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC 
CATGGCTCCT CCAGCCCCTC GTCCCCGCTC TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG 
ACTCCCAATG AGAGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGCCTCCT CACGCACACG 
TTCAACCGAG AGTACACCCA CAGCCACGTC TGCATCAGTG CCAGCGAGAG CAAGCTCTCC 
GAGATGTCTG TCACCCTGCT TCGGGACCCA TCCATGTCCC CGCTGGGGGC GGCCACCCTC 
ACCCCCTCCT CCACCTGCCC CTCTCTGGTC GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG 
ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT 
TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTTCCCG ACATCCAGGA GATCCGGGTC AGCCCCATCG TGTCTAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC 
TACGCCTACC TGTACAACAG CGACAAGGAC TCCGTGGAGA GGTTCGTGCT CAACCTGTCC 
ACCGCGCAGG TGGAGTACAG CGAGGACCAG CAGGCCATGC TCAAGACGCC CAACACGTTC 
GCCGTGTGCA CGGAGCACCG CGGCATCCTG CTGCAAGCCA ACAGCGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCGCTCCTG GCCGGGACCA TACGGTCCAA GCTCTCCAGA 
AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265283.1
CDS	207..5333
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409575.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409575.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGGA CCTTCTACCA ATTCGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCTCTT CTGCTCAACC GGGTCACCCC GTACCGAGAG 
AAGATCTACA TGACTCTCTC AGCTTACATT GAGATGGAGA ACTGCACTCA GCCGGCCGTC 
ATCACCAAGG ACTTCTGCAT GGTCTTCTAC TCTCGGGACG CCAAGCTGCC CGCTTCCCGC 
TCCATCCGCA ACCTGTTTGG CAGCGGGAGC CTCCGGGCCT CGGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTCAGCCT GTGCCATGTG GCCGACGCCG GCAGCCCAGG GATGCAGCGG 
CGGCGGCGGC GCGTGCTGGA TACATCCGTG GCCTACGTGC GGGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGGAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTTGA GAAGACCAGA CACTACCTTC TGCTGCGGGA GAAGCTGGAG 
ACCACCCAGC GGCCCGGCCC TGAGGCGCCG TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC 
CATGGCTCCT CCAGCCCCTC GTCCCCGCTC TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG 
ACTCCCAATG AGAGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGCCTCCT CACGCACACG 
TTCAACCGAG AGTACACCCA CAGCCACGTC TGCATCAGTG CCAGCGAGAG CAAGCTCTCC 
GAGATGTCTG TCACCCTGCT TCGGGACCCA TCCATGTCCC CGCTGGGGGC GGCCACCCTC 
ACCCCCTCCT CCACCTGCCC CTCTCTGGTC GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG 
ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT 
TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTTCCCG ACATCCAGGA GATCCGGGTC AGCCCCATCG TGTCTAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC 
TACGCCTACC TGTACAACAG CGACAAGGAC TCCGTGGAGA GGTTCGTGCT CAACCTGTCC 
ACCGCGCAGG TGGAGTACAG CGAGGACCAG CAGGCCATGC TCAAGACGCC CAACACGTTC 
GCCGTGTGCA CGGAGCACCG CGGCATCCTG CTGCAAGCCA ACAGCGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCGCTCCTG GCCGGGACCA TACGGTCCAA GCTCTCCAGA 
AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107687.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963162.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

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CloneID	OCe43171
Clone ID Related Accession (Same CDS sequence)	XM_014107687.1
Accession Version	XM_014107687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGGACC 
TTCTACCAAT TCGAGGCTGC GTGGGACAGC TCCATGCACA ACTCTCTTCT GCTCAACCGG 
GTCACCCCGT ACCGAGAGAA GATCTACATG ACTCTCTCAG CTTACATTGA GATGGAGAAC 
TGCACTCAGC CGGCCGTCAT CACCAAGGAC TTCTGCATGG TCTTCTACTC TCGGGACGCC 
AAGCTGCCCG CTTCCCGCTC CATCCGCAAC CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTCAGCCTGT GCCATGTGGC CGACGCCGGC 
AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC GTGCTGGATA CATCCGTGGC CTACGTGCGG 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGGAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTTGAGA AGACCAGACA CTACCTTCTG 
CTGCGGGAGA AGCTGGAGAC CACCCAGCGG CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC 
AGCGAGGACT TGGGGTCCCA TGGCTCCTCC AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC 
CGCCCGTCGC CTCTGGAGAC TCCCAATGAG AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGCCTCCTCA CGCACACGTT CAACCGAGAG TACACCCACA GCCACGTCTG CATCAGTGCC 
AGCGAGAGCA AGCTCTCCGA GATGTCTGTC ACCCTGCTTC GGGACCCATC CATGTCCCCG 
CTGGGGGCGG CCACCCTCAC CCCCTCCTCC ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC 
GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG 
CCTGTGCCAG AGGCAGATTC CAAGAAGCTT CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC ATCCAGGAGA TCCGGGTCAG CCCCATCGTG 
TCTAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTAC GGCGGCCCTA CGCCTACCTG TACAACAGCG ACAAGGACTC CGTGGAGAGG 
TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG GAGTACAGCG AGGACCAGCA GGCCATGCTC 
AAGACGCCCA ACACGTTCGC CGTGTGCACG GAGCACCGCG GCATCCTGCT GCAAGCCAAC 
AGCGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CGCTCCTGGC CGGGACCATA 
CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963162.1
CDS	193..5397
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107687.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107687.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTC TACCTGAGCA ACCTGCTGTA CCCTGTCCCC 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG 
GCCGTGCAGG CCACCTCAGC CGACGAAGAG GCCCCCGACT ATGGCTCTGG CGTCCGACAG 
TCCGGCACGG CCAGAATCTC CTTTGACGAC CAGCATTTTG AAAAGTTCCA GTCTGAGTCC 
TGCCCTGTGG TGGGGATGTC CCGCTCGGGG ACCTCCCAGG AAGAGCTGCG CATCGTGGAG 
GGTCAGGGCC AGGGTGCAGA CACCGGGCCC TCGGCCGACG AGGTCAACAA CAACACCTGC 
TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG GACAGCCCCG AGAAGGCCGC CACAGATGGG 
CCTCTGGACG CCGCCCTGGA CCACCTGCGC CTGGGCAGCA CCTTCACCTT CCGCGTGACT 
GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA TACGCCGACA TTTTCTGCCA GTTCAACTTC 
ATCCACCGCC ACGACGAGGC CTTCTCCACG GAGCCCCTCA AGAACACAGG GAGGGGCCCC 
CCGCTTGGCT TCTACCACGT CCAGAACATC GCAGTGGAGG TGACCAAGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCAT CGTGTTCGAG GTCTTCGGCC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGATGT GCTCAGCCCC CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG 
GTCATGCCGC TGTCCAAGCC AGTGCCTGCC ACCAAGCTCA GCACGCTAGC GCGACCCTGC 
CCTGGGCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TTGAGATCTG TGAGCTGGAG 
GCCAACGGCG ATTACATTCC CGCAGTGGTG GACCACCGTG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TGCACCAGGG CATCCAGAGA CGGATCACCG TGACGCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTTCGA GAGCTGGTCG TGGGTCGGAT CCGAAACACT 
CCCGAAACCG ATGAGTCCCT GATCGACCCC AACATCTTGT CGCTCAACAT CCTCTCCTCC 
GATTATATCC ACCCGGCCCA GGACGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGGACC 
TTCTACCAAT TCGAGGCTGC GTGGGACAGC TCCATGCACA ACTCTCTTCT GCTCAACCGG 
GTCACCCCGT ACCGAGAGAA GATCTACATG ACTCTCTCAG CTTACATTGA GATGGAGAAC 
TGCACTCAGC CGGCCGTCAT CACCAAGGAC TTCTGCATGG TCTTCTACTC TCGGGACGCC 
AAGCTGCCCG CTTCCCGCTC CATCCGCAAC CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTCAGCCTGT GCCATGTGGC CGACGCCGGC 
AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC GTGCTGGATA CATCCGTGGC CTACGTGCGG 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGGAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTTGAGA AGACCAGACA CTACCTTCTG 
CTGCGGGAGA AGCTGGAGAC CACCCAGCGG CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC 
AGCGAGGACT TGGGGTCCCA TGGCTCCTCC AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC 
CGCCCGTCGC CTCTGGAGAC TCCCAATGAG AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGCCTCCTCA CGCACACGTT CAACCGAGAG TACACCCACA GCCACGTCTG CATCAGTGCC 
AGCGAGAGCA AGCTCTCCGA GATGTCTGTC ACCCTGCTTC GGGACCCATC CATGTCCCCG 
CTGGGGGCGG CCACCCTCAC CCCCTCCTCC ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC 
GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG 
CCTGTGCCAG AGGCAGATTC CAAGAAGCTT CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC ATCCAGGAGA TCCGGGTCAG CCCCATCGTG 
TCTAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTAC GGCGGCCCTA CGCCTACCTG TACAACAGCG ACAAGGACTC CGTGGAGAGG 
TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG GAGTACAGCG AGGACCAGCA GGCCATGCTC 
AAGACGCCCA ACACGTTCGC CGTGTGCACG GAGCACCGCG GCATCCTGCT GCAAGCCAAC 
AGCGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CGCTCCTGGC CGGGACCATA 
CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( NM_001168473.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001161945.2 Canis lupus familiaris kinesin family member 1A (KIF1A), mRNA.

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CloneID	OCe25957
Clone ID Related Accession (Same CDS sequence)	XM_005635953.2 , NM_001168473.2 , XM_005635953.3
Accession Version	NM_001168473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03014559.1. On Sep 2, 2015 this sequence version replaced NM_001168473.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GU014399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161945.2
CDS	1..5073
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	NM_001168473.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168473.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635951.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636008.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

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CloneID	OCe25955
Clone ID Related Accession (Same CDS sequence)	XM_005635951.2 , XM_005635951.3
Accession Version	XM_005635951.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGACCTTCTA CCAATTCGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCT 
CTTCTGCTCA ACCGGGTCAC CCCGTACCGA GAGAAGATCT ACATGACTCT CTCAGCTTAC 
ATTGAGATGG AGAACTGCAC TCAGCCGGCC GTCATCACCA AGGACTTCTG CATGGTCTTC 
TACTCTCGGG ACGCCAAGCT GCCCGCTTCC CGCTCCATCC GCAACCTGTT TGGCAGCGGG 
AGCCTCCGGG CCTCGGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT 
GTGGCCGACG CCGGCAGCCC AGGGATGCAG CGGCGGCGGC GGCGCGTGCT GGATACATCC 
GTGGCCTACG TGCGGGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGGAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT TGAGAAGACC 
AGACACTACC TTCTGCTGCG GGAGAAGCTG GAGACCACCC AGCGGCCCGG CCCTGAGGCG 
CCGTCCCCCG CCTCCAGCGA GGACTTGGGG TCCCATGGCT CCTCCAGCCC CTCGTCCCCG 
CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG GAGACTCCCA ATGAGAGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGCCT CCTCACGCAC ACGTTCAACC GAGAGTACAC CCACAGCCAC 
GTCTGCATCA GTGCCAGCGA GAGCAAGCTC TCCGAGATGT CTGTCACCCT GCTTCGGGAC 
CCATCCATGT CCCCGCTGGG GGCGGCCACC CTCACCCCCT CCTCCACCTG CCCCTCTCTG 
GTCGAAGGGC GGTACGGAGC CGCTGAGCTG AGGACCCCCC AGCCCTGCTC CCGGCCCGCC 
AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA GATTCCAAGA AGCTTCCCTC CCCTGCTCGG 
GCTGCGGAGG CCGACAAGGA GCCCCAGCGC CTGCTGGTTC CCGACATCCA GGAGATCCGG 
GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTACGGCGG CCCTACGCCT ACCTGTACAA CAGCGACAAG 
GACTCCGTGG AGAGGTTCGT GCTCAACCTG TCCACCGCGC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCCA TGCTCAAGAC GCCCAACACG TTCGCCGTGT GCACGGAGCA CCGCGGCATC 
CTGCTGCAAG CCAACAGCGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCGCTC 
CTGGCCGGGA CCATACGGTC CAAGCTCTCC AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636008.1
CDS	192..5291
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635951.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635951.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGACCTTCTA CCAATTCGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCT 
CTTCTGCTCA ACCGGGTCAC CCCGTACCGA GAGAAGATCT ACATGACTCT CTCAGCTTAC 
ATTGAGATGG AGAACTGCAC TCAGCCGGCC GTCATCACCA AGGACTTCTG CATGGTCTTC 
TACTCTCGGG ACGCCAAGCT GCCCGCTTCC CGCTCCATCC GCAACCTGTT TGGCAGCGGG 
AGCCTCCGGG CCTCGGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT 
GTGGCCGACG CCGGCAGCCC AGGGATGCAG CGGCGGCGGC GGCGCGTGCT GGATACATCC 
GTGGCCTACG TGCGGGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGGAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT TGAGAAGACC 
AGACACTACC TTCTGCTGCG GGAGAAGCTG GAGACCACCC AGCGGCCCGG CCCTGAGGCG 
CCGTCCCCCG CCTCCAGCGA GGACTTGGGG TCCCATGGCT CCTCCAGCCC CTCGTCCCCG 
CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG GAGACTCCCA ATGAGAGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGCCT CCTCACGCAC ACGTTCAACC GAGAGTACAC CCACAGCCAC 
GTCTGCATCA GTGCCAGCGA GAGCAAGCTC TCCGAGATGT CTGTCACCCT GCTTCGGGAC 
CCATCCATGT CCCCGCTGGG GGCGGCCACC CTCACCCCCT CCTCCACCTG CCCCTCTCTG 
GTCGAAGGGC GGTACGGAGC CGCTGAGCTG AGGACCCCCC AGCCCTGCTC CCGGCCCGCC 
AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA GATTCCAAGA AGCTTCCCTC CCCTGCTCGG 
GCTGCGGAGG CCGACAAGGA GCCCCAGCGC CTGCTGGTTC CCGACATCCA GGAGATCCGG 
GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTACGGCGG CCCTACGCCT ACCTGTACAA CAGCGACAAG 
GACTCCGTGG AGAGGTTCGT GCTCAACCTG TCCACCGCGC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCCA TGCTCAAGAC GCCCAACACG TTCGCCGTGT GCACGGAGCA CCGCGGCATC 
CTGCTGCAAG CCAACAGCGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCGCTC 
CTGGCCGGGA CCATACGGTC CAAGCTCTCC AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635951.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636008.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

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CloneID	OCe25955
Clone ID Related Accession (Same CDS sequence)	XM_005635951.2 , XM_005635951.3
Accession Version	XM_005635951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGACCTTCTA CCAATTCGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCT 
CTTCTGCTCA ACCGGGTCAC CCCGTACCGA GAGAAGATCT ACATGACTCT CTCAGCTTAC 
ATTGAGATGG AGAACTGCAC TCAGCCGGCC GTCATCACCA AGGACTTCTG CATGGTCTTC 
TACTCTCGGG ACGCCAAGCT GCCCGCTTCC CGCTCCATCC GCAACCTGTT TGGCAGCGGG 
AGCCTCCGGG CCTCGGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT 
GTGGCCGACG CCGGCAGCCC AGGGATGCAG CGGCGGCGGC GGCGCGTGCT GGATACATCC 
GTGGCCTACG TGCGGGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGGAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT TGAGAAGACC 
AGACACTACC TTCTGCTGCG GGAGAAGCTG GAGACCACCC AGCGGCCCGG CCCTGAGGCG 
CCGTCCCCCG CCTCCAGCGA GGACTTGGGG TCCCATGGCT CCTCCAGCCC CTCGTCCCCG 
CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG GAGACTCCCA ATGAGAGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGCCT CCTCACGCAC ACGTTCAACC GAGAGTACAC CCACAGCCAC 
GTCTGCATCA GTGCCAGCGA GAGCAAGCTC TCCGAGATGT CTGTCACCCT GCTTCGGGAC 
CCATCCATGT CCCCGCTGGG GGCGGCCACC CTCACCCCCT CCTCCACCTG CCCCTCTCTG 
GTCGAAGGGC GGTACGGAGC CGCTGAGCTG AGGACCCCCC AGCCCTGCTC CCGGCCCGCC 
AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA GATTCCAAGA AGCTTCCCTC CCCTGCTCGG 
GCTGCGGAGG CCGACAAGGA GCCCCAGCGC CTGCTGGTTC CCGACATCCA GGAGATCCGG 
GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTACGGCGG CCCTACGCCT ACCTGTACAA CAGCGACAAG 
GACTCCGTGG AGAGGTTCGT GCTCAACCTG TCCACCGCGC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCCA TGCTCAAGAC GCCCAACACG TTCGCCGTGT GCACGGAGCA CCGCGGCATC 
CTGCTGCAAG CCAACAGCGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCGCTC 
CTGGCCGGGA CCATACGGTC CAAGCTCTCC AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636008.1
CDS	207..5306
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635951.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635951.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TCTACCTGAG CAACCTGCTG TACCCTGTCC CCCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC TTCCTCCGCG TGGCCGTGCA GGCCACCTCA 
GCCGACGAAG AGGCCCCCGA CTATGGCTCT GGCGTCCGAC AGTCCGGCAC GGCCAGAATC 
TCCTTTGACG ACCAGCATTT TGAAAAGTTC CAGTCTGAGT CCTGCCCTGT GGTGGGGATG 
TCCCGCTCGG GGACCTCCCA GGAAGAGCTG CGCATCGTGG AGGGTCAGGG CCAGGGTGCA 
GACACCGGGC CCTCGGCCGA CGAGGTCAAC AACAACACCT GCTCAGCCGT GCCCCCGGAA 
GGCCTCCTCC TGGACAGCCC CGAGAAGGCC GCCACAGATG GGCCTCTGGA CGCCGCCCTG 
GACCACCTGC GCCTGGGCAG CACCTTCACC TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC 
ATCTCCGCCG AATACGCCGA CATTTTCTGC CAGTTCAACT TCATCCACCG CCACGACGAG 
GCCTTCTCCA CGGAGCCCCT CAAGAACACA GGGAGGGGCC CCCCGCTTGG CTTCTACCAC 
GTCCAGAACA TCGCAGTGGA GGTGACCAAG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
ATCGTGTTCG AGGTCTTCGG CCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAT 
GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC CACTTCCCGA GGGTCATGCC GCTGTCCAAG 
CCAGTGCCTG CCACCAAGCT CAGCACGCTA GCGCGACCCT GCCCTGGGCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTTGAGATC TGTGAGCTGG AGGCCAACGG CGATTACATT 
CCCGCAGTGG TGGACCACCG TGGCGGGATG CCGTGCATGG GGACCTTCCT GCTGCACCAG 
GGCATCCAGA GACGGATCAC CGTGACGCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG ATCCGAAACA CTCCCGAAAC CGATGAGTCC 
CTGATCGACC CCAACATCTT GTCGCTCAAC ATCCTCTCCT CCGATTATAT CCACCCGGCC 
CAGGACGACC GGACCTTCTA CCAATTCGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCT 
CTTCTGCTCA ACCGGGTCAC CCCGTACCGA GAGAAGATCT ACATGACTCT CTCAGCTTAC 
ATTGAGATGG AGAACTGCAC TCAGCCGGCC GTCATCACCA AGGACTTCTG CATGGTCTTC 
TACTCTCGGG ACGCCAAGCT GCCCGCTTCC CGCTCCATCC GCAACCTGTT TGGCAGCGGG 
AGCCTCCGGG CCTCGGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT 
GTGGCCGACG CCGGCAGCCC AGGGATGCAG CGGCGGCGGC GGCGCGTGCT GGATACATCC 
GTGGCCTACG TGCGGGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGGAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT TGAGAAGACC 
AGACACTACC TTCTGCTGCG GGAGAAGCTG GAGACCACCC AGCGGCCCGG CCCTGAGGCG 
CCGTCCCCCG CCTCCAGCGA GGACTTGGGG TCCCATGGCT CCTCCAGCCC CTCGTCCCCG 
CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG GAGACTCCCA ATGAGAGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGCCT CCTCACGCAC ACGTTCAACC GAGAGTACAC CCACAGCCAC 
GTCTGCATCA GTGCCAGCGA GAGCAAGCTC TCCGAGATGT CTGTCACCCT GCTTCGGGAC 
CCATCCATGT CCCCGCTGGG GGCGGCCACC CTCACCCCCT CCTCCACCTG CCCCTCTCTG 
GTCGAAGGGC GGTACGGAGC CGCTGAGCTG AGGACCCCCC AGCCCTGCTC CCGGCCCGCC 
AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA GATTCCAAGA AGCTTCCCTC CCCTGCTCGG 
GCTGCGGAGG CCGACAAGGA GCCCCAGCGC CTGCTGGTTC CCGACATCCA GGAGATCCGG 
GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTACGGCGG CCCTACGCCT ACCTGTACAA CAGCGACAAG 
GACTCCGTGG AGAGGTTCGT GCTCAACCTG TCCACCGCGC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCCA TGCTCAAGAC GCCCAACACG TTCGCCGTGT GCACGGAGCA CCGCGGCATC 
CTGCTGCAAG CCAACAGCGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCGCTC 
CTGGCCGGGA CCATACGGTC CAAGCTCTCC AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635952.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636009.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

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CloneID	OCe25956
Clone ID Related Accession (Same CDS sequence)	XM_005635952.2 , XM_005635952.3
Accession Version	XM_005635952.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGCGTTTC ATTCGGAAAT 
GACACTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636009.1
CDS	193..5289
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635952.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635952.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGCGTTTC ATTCGGAAAT 
GACACTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635952.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636009.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

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CloneID	OCe25956
Clone ID Related Accession (Same CDS sequence)	XM_005635952.2 , XM_005635952.3
Accession Version	XM_005635952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGCGTTTC ATTCGGAAAT 
GACACTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636009.1
CDS	207..5303
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635952.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635952.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGCGTTTC ATTCGGAAAT 
GACACTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635953.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636010.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

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CloneID	OCe25957
Clone ID Related Accession (Same CDS sequence)	XM_005635953.2 , NM_001168473.2 , XM_005635953.3
Accession Version	XM_005635953.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636010.1
CDS	193..5265
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635953.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635953.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635953.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636010.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X15, mRNA.

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CloneID	OCe25957
Clone ID Related Accession (Same CDS sequence)	XM_005635953.2 , NM_001168473.2 , XM_005635953.3
Accession Version	XM_005635953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636010.1
CDS	207..5279
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635953.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635953.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTCTACCT GAGCAACCTG 
CTGTACCCTG TCCCCCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG 
GGCTTCCTCC GCGTGGCCGT GCAGGCCACC TCAGCCGACG AAGAGGCCCC CGACTATGGC 
TCTGGCGTCC GACAGTCCGG CACGGCCAGA ATCTCCTTTG ACGACCAGCA TTTTGAAAAG 
TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG ATGTCCCGCT CGGGGACCTC CCAGGAAGAG 
CTGCGCATCG TGGAGGGTCA GGGCCAGGGT GCAGACACCG GGCCCTCGGC CGACGAGGTC 
AACAACAACA CCTGCTCAGC CGTGCCCCCG GAAGGCCTCC TCCTGGACAG CCCCGAGAAG 
GCCGCCACAG ATGGGCCTCT GGACGCCGCC CTGGACCACC TGCGCCTGGG CAGCACCTTC 
ACCTTCCGCG TGACTGTCCT GCAGGCGTCC AGCATCTCCG CCGAATACGC CGACATTTTC 
TGCCAGTTCA ACTTCATCCA CCGCCACGAC GAGGCCTTCT CCACGGAGCC CCTCAAGAAC 
ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC CACGTCCAGA ACATCGCAGT GGAGGTGACC 
AAGTCCTTCA TCGAGTACAT CAAGAGCCAG CCCATCGTGT TCGAGGTCTT CGGCCACTAC 
CAGCAGCACC CGTTCCCGCC CCTCTGCAAG GATGTGCTCA GCCCCCTGAG GCCCTCGCGC 
CGCCACTTCC CGAGGGTCAT GCCGCTGTCC AAGCCAGTGC CTGCCACCAA GCTCAGCACG 
CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC TGCAAGTACG ACCTGCTGGT CTACTTTGAG 
ATCTGTGAGC TGGAGGCCAA CGGCGATTAC ATTCCCGCAG TGGTGGACCA CCGTGGCGGG 
ATGCCGTGCA TGGGGACCTT CCTGCTGCAC CAGGGCATCC AGAGACGGAT CACCGTGACG 
CTGCTGCACG AGACGGGCAG CCACATCCGC TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT 
CGGATCCGAA ACACTCCCGA AACCGATGAG TCCCTGATCG ACCCCAACAT CTTGTCGCTC 
AACATCCTCT CCTCCGATTA TATCCACCCG GCCCAGGACG ACCGGACCTT CTACCAATTC 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCTCTTCTGC TCAACCGGGT CACCCCGTAC 
CGAGAGAAGA TCTACATGAC TCTCTCAGCT TACATTGAGA TGGAGAACTG CACTCAGCCG 
GCCGTCATCA CCAAGGACTT CTGCATGGTC TTCTACTCTC GGGACGCCAA GCTGCCCGCT 
TCCCGCTCCA TCCGCAACCT GTTTGGCAGC GGGAGCCTCC GGGCCTCGGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT CAGCCTGTGC CATGTGGCCG ACGCCGGCAG CCCAGGGATG 
CAGCGGCGGC GGCGGCGCGT GCTGGATACA TCCGTGGCCT ACGTGCGGGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG GAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTTGAGAAG ACCAGACACT ACCTTCTGCT GCGGGAGAAG 
CTGGAGACCA CCCAGCGGCC CGGCCCTGAG GCGCCGTCCC CCGCCTCCAG CGAGGACTTG 
GGGTCCCATG GCTCCTCCAG CCCCTCGTCC CCGCTCTCAG CTGAGGGCCG CCCGTCGCCT 
CTGGAGACTC CCAATGAGAG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG CCTCCTCACG 
CACACGTTCA ACCGAGAGTA CACCCACAGC CACGTCTGCA TCAGTGCCAG CGAGAGCAAG 
CTCTCCGAGA TGTCTGTCAC CCTGCTTCGG GACCCATCCA TGTCCCCGCT GGGGGCGGCC 
ACCCTCACCC CCTCCTCCAC CTGCCCCTCT CTGGTCGAAG GGCGGTACGG AGCCGCTGAG 
CTGAGGACCC CCCAGCCCTG CTCCCGGCCC GCCAGCCCAG AGCCTGAGCC TGTGCCAGAG 
GCAGATTCCA AGAAGCTTCC CTCCCCTGCT CGGGCTGCGG AGGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TTCCCGACAT CCAGGAGATC CGGGTCAGCC CCATCGTGTC TAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTACGG 
CGGCCCTACG CCTACCTGTA CAACAGCGAC AAGGACTCCG TGGAGAGGTT CGTGCTCAAC 
CTGTCCACCG CGCAGGTGGA GTACAGCGAG GACCAGCAGG CCATGCTCAA GACGCCCAAC 
ACGTTCGCCG TGTGCACGGA GCACCGCGGC ATCCTGCTGC AAGCCAACAG CGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCG CTCCTGGCCG GGACCATACG GTCCAAGCTC 
TCCAGAAGGC GGTCTGCCCA GATGAGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635949.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636006.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe25954
Clone ID Related Accession (Same CDS sequence)	XM_005635949.2 , XM_005635949.3
Accession Version	XM_005635949.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636006.1
CDS	194..5563
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635949.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635949.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005635949.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636006.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

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CloneID	OCe25954
Clone ID Related Accession (Same CDS sequence)	XM_005635949.2 , XM_005635949.3
Accession Version	XM_005635949.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636006.1
CDS	207..5576
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005635949.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635949.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409568.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265276.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

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CloneID	OCe297010
Clone ID Related Accession (Same CDS sequence)	XM_022409568.1
Accession Version	XM_022409568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGCGTT TCATTCGGAA ATGACACTAG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265276.1
CDS	207..5705
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409568.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409568.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGCGTT TCATTCGGAA ATGACACTAG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409569.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265277.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

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CloneID	OCe297011
Clone ID Related Accession (Same CDS sequence)	XM_022409569.1
Accession Version	XM_022409569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG CTACCCTCTT CTCAACACAT GCATGAGCGA GCGCATGGCT 
GCTCTCACCC CCTCCCCCAC CTTCTCGAGC CCCGACTCCG ACGCCACCGA GCCTGCCGAG 
GAGCAGAGCG TGGGGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGACCTG 
GAGGACGACG TCTTTCCGGA GCACGCGCTG TGCGACGGCC GGGACCCGTT TTACGACCGG 
CCCCCCCTGT TCAGTTTAGT AGGAAGGGCC TTCGTCTACC TGAGCAACCT GCTGTACCCT 
GTCCCCCTGG TGCACCGCGT GGCCGTCGTC AGCGAGAAGG GAGAGGTGAA GGGCTTCCTC 
CGCGTGGCCG TGCAGGCCAC CTCAGCCGAC GAAGAGGCCC CCGACTATGG CTCTGGCGTC 
CGACAGTCCG GCACGGCCAG AATCTCCTTT GACGACCAGC ATTTTGAAAA GTTCCAGTCT 
GAGTCCTGCC CTGTGGTGGG GATGTCCCGC TCGGGGACCT CCCAGGAAGA GCTGCGCATC 
GTGGAGGGTC AGGGCCAGGG TGCAGACACC GGGCCCTCGG CCGACGAGGT CAACAACAAC 
ACCTGCTCAG CCGTGCCCCC GGAAGGCCTC CTCCTGGACA GCCCCGAGAA GGCCGCCACA 
GATGGGCCTC TGGACGCCGC CCTGGACCAC CTGCGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACTGTCC TGCAGGCGTC CAGCATCTCC GCCGAATACG CCGACATTTT CTGCCAGTTC 
AACTTCATCC ACCGCCACGA CGAGGCCTTC TCCACGGAGC CCCTCAAGAA CACAGGGAGG 
GGCCCCCCGC TTGGCTTCTA CCACGTCCAG AACATCGCAG TGGAGGTGAC CAAGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCATCGTG TTCGAGGTCT TCGGCCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGATGTGCTC AGCCCCCTGA GGCCCTCGCG CCGCCACTTC 
CCGAGGGTCA TGCCGCTGTC CAAGCCAGTG CCTGCCACCA AGCTCAGCAC GCTAGCGCGA 
CCCTGCCCTG GGCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTTGA GATCTGTGAG 
CTGGAGGCCA ACGGCGATTA CATTCCCGCA GTGGTGGACC ACCGTGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTGCA CCAGGGCATC CAGAGACGGA TCACCGTGAC GCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTTCGAGAGC TGGTCGTGGG TCGGATCCGA 
AACACTCCCG AAACCGATGA GTCCCTGATC GACCCCAACA TCTTGTCGCT CAACATCCTC 
TCCTCCGATT ATATCCACCC GGCCCAGGAC GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGCGTTTCAT TCGGAAATGA CACTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265277.1
CDS	207..5681
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409569.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409569.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG CTACCCTCTT CTCAACACAT GCATGAGCGA GCGCATGGCT 
GCTCTCACCC CCTCCCCCAC CTTCTCGAGC CCCGACTCCG ACGCCACCGA GCCTGCCGAG 
GAGCAGAGCG TGGGGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGACCTG 
GAGGACGACG TCTTTCCGGA GCACGCGCTG TGCGACGGCC GGGACCCGTT TTACGACCGG 
CCCCCCCTGT TCAGTTTAGT AGGAAGGGCC TTCGTCTACC TGAGCAACCT GCTGTACCCT 
GTCCCCCTGG TGCACCGCGT GGCCGTCGTC AGCGAGAAGG GAGAGGTGAA GGGCTTCCTC 
CGCGTGGCCG TGCAGGCCAC CTCAGCCGAC GAAGAGGCCC CCGACTATGG CTCTGGCGTC 
CGACAGTCCG GCACGGCCAG AATCTCCTTT GACGACCAGC ATTTTGAAAA GTTCCAGTCT 
GAGTCCTGCC CTGTGGTGGG GATGTCCCGC TCGGGGACCT CCCAGGAAGA GCTGCGCATC 
GTGGAGGGTC AGGGCCAGGG TGCAGACACC GGGCCCTCGG CCGACGAGGT CAACAACAAC 
ACCTGCTCAG CCGTGCCCCC GGAAGGCCTC CTCCTGGACA GCCCCGAGAA GGCCGCCACA 
GATGGGCCTC TGGACGCCGC CCTGGACCAC CTGCGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACTGTCC TGCAGGCGTC CAGCATCTCC GCCGAATACG CCGACATTTT CTGCCAGTTC 
AACTTCATCC ACCGCCACGA CGAGGCCTTC TCCACGGAGC CCCTCAAGAA CACAGGGAGG 
GGCCCCCCGC TTGGCTTCTA CCACGTCCAG AACATCGCAG TGGAGGTGAC CAAGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCATCGTG TTCGAGGTCT TCGGCCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGATGTGCTC AGCCCCCTGA GGCCCTCGCG CCGCCACTTC 
CCGAGGGTCA TGCCGCTGTC CAAGCCAGTG CCTGCCACCA AGCTCAGCAC GCTAGCGCGA 
CCCTGCCCTG GGCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTTGA GATCTGTGAG 
CTGGAGGCCA ACGGCGATTA CATTCCCGCA GTGGTGGACC ACCGTGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTGCA CCAGGGCATC CAGAGACGGA TCACCGTGAC GCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTTCGAGAGC TGGTCGTGGG TCGGATCCGA 
AACACTCCCG AAACCGATGA GTCCCTGATC GACCCCAACA TCTTGTCGCT CAACATCCTC 
TCCTCCGATT ATATCCACCC GGCCCAGGAC GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGCGTTTCAT TCGGAAATGA CACTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409570.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265278.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

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CloneID	OCe297012
Clone ID Related Accession (Same CDS sequence)	XM_022409570.1
Accession Version	XM_022409570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGCAGTTTCT TGATTCGGAC 
ATGCCTAGCG TTTCATTCGG AAATGACACT AGGACCTTCT ACCAATTCGA GGCTGCGTGG 
GACAGCTCCA TGCACAACTC TCTTCTGCTC AACCGGGTCA CCCCGTACCG AGAGAAGATC 
TACATGACTC TCTCAGCTTA CATTGAGATG GAGAACTGCA CTCAGCCGGC CGTCATCACC 
AAGGACTTCT GCATGGTCTT CTACTCTCGG GACGCCAAGC TGCCCGCTTC CCGCTCCATC 
CGCAACCTGT TTGGCAGCGG GAGCCTCCGG GCCTCGGAGA GCAACCGCGT GACGGGCGTG 
TACGAGCTCA GCCTGTGCCA TGTGGCCGAC GCCGGCAGCC CAGGGATGCA GCGGCGGCGG 
CGGCGCGTGC TGGATACATC CGTGGCCTAC GTGCGGGGCG AGGAGAACCT GGCGGGCTGG 
AGGCCGCGGA GTGACAGCCT CATCCTGGAC CACCAGTGGG AGCTGGAGAA GCTGAGTCTC 
CTGCAGGAGG TTGAGAAGAC CAGACACTAC CTTCTGCTGC GGGAGAAGCT GGAGACCACC 
CAGCGGCCCG GCCCTGAGGC GCCGTCCCCC GCCTCCAGCG AGGACTTGGG GTCCCATGGC 
TCCTCCAGCC CCTCGTCCCC GCTCTCAGCT GAGGGCCGCC CGTCGCCTCT GGAGACTCCC 
AATGAGAGGC AGCGGGAGCT GGCTGTCAAG TGCTTACGCC TCCTCACGCA CACGTTCAAC 
CGAGAGTACA CCCACAGCCA CGTCTGCATC AGTGCCAGCG AGAGCAAGCT CTCCGAGATG 
TCTGTCACCC TGCTTCGGGA CCCATCCATG TCCCCGCTGG GGGCGGCCAC CCTCACCCCC 
TCCTCCACCT GCCCCTCTCT GGTCGAAGGG CGGTACGGAG CCGCTGAGCT GAGGACCCCC 
CAGCCCTGCT CCCGGCCCGC CAGCCCAGAG CCTGAGCCTG TGCCAGAGGC AGATTCCAAG 
AAGCTTCCCT CCCCTGCTCG GGCTGCGGAG GCCGACAAGG AGCCCCAGCG CCTGCTGGTT 
CCCGACATCC AGGAGATCCG GGTCAGCCCC ATCGTGTCTA AGAAGGGCTA CCTGCACTTC 
CTGGAGCCGC ACACGGCCGG CTGGGCCAAG CGCTTCGTGG TGGTACGGCG GCCCTACGCC 
TACCTGTACA ACAGCGACAA GGACTCCGTG GAGAGGTTCG TGCTCAACCT GTCCACCGCG 
CAGGTGGAGT ACAGCGAGGA CCAGCAGGCC ATGCTCAAGA CGCCCAACAC GTTCGCCGTG 
TGCACGGAGC ACCGCGGCAT CCTGCTGCAA GCCAACAGCG ACAAGGACAT GCACGACTGG 
CTGTACGCCT TCAACCCGCT CCTGGCCGGG ACCATACGGT CCAAGCTCTC CAGAAGGCGG 
TCTGCCCAGA TGAGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265278.1
CDS	207..5627
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409570.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409570.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGCAGTTTCT TGATTCGGAC 
ATGCCTAGCG TTTCATTCGG AAATGACACT AGGACCTTCT ACCAATTCGA GGCTGCGTGG 
GACAGCTCCA TGCACAACTC TCTTCTGCTC AACCGGGTCA CCCCGTACCG AGAGAAGATC 
TACATGACTC TCTCAGCTTA CATTGAGATG GAGAACTGCA CTCAGCCGGC CGTCATCACC 
AAGGACTTCT GCATGGTCTT CTACTCTCGG GACGCCAAGC TGCCCGCTTC CCGCTCCATC 
CGCAACCTGT TTGGCAGCGG GAGCCTCCGG GCCTCGGAGA GCAACCGCGT GACGGGCGTG 
TACGAGCTCA GCCTGTGCCA TGTGGCCGAC GCCGGCAGCC CAGGGATGCA GCGGCGGCGG 
CGGCGCGTGC TGGATACATC CGTGGCCTAC GTGCGGGGCG AGGAGAACCT GGCGGGCTGG 
AGGCCGCGGA GTGACAGCCT CATCCTGGAC CACCAGTGGG AGCTGGAGAA GCTGAGTCTC 
CTGCAGGAGG TTGAGAAGAC CAGACACTAC CTTCTGCTGC GGGAGAAGCT GGAGACCACC 
CAGCGGCCCG GCCCTGAGGC GCCGTCCCCC GCCTCCAGCG AGGACTTGGG GTCCCATGGC 
TCCTCCAGCC CCTCGTCCCC GCTCTCAGCT GAGGGCCGCC CGTCGCCTCT GGAGACTCCC 
AATGAGAGGC AGCGGGAGCT GGCTGTCAAG TGCTTACGCC TCCTCACGCA CACGTTCAAC 
CGAGAGTACA CCCACAGCCA CGTCTGCATC AGTGCCAGCG AGAGCAAGCT CTCCGAGATG 
TCTGTCACCC TGCTTCGGGA CCCATCCATG TCCCCGCTGG GGGCGGCCAC CCTCACCCCC 
TCCTCCACCT GCCCCTCTCT GGTCGAAGGG CGGTACGGAG CCGCTGAGCT GAGGACCCCC 
CAGCCCTGCT CCCGGCCCGC CAGCCCAGAG CCTGAGCCTG TGCCAGAGGC AGATTCCAAG 
AAGCTTCCCT CCCCTGCTCG GGCTGCGGAG GCCGACAAGG AGCCCCAGCG CCTGCTGGTT 
CCCGACATCC AGGAGATCCG GGTCAGCCCC ATCGTGTCTA AGAAGGGCTA CCTGCACTTC 
CTGGAGCCGC ACACGGCCGG CTGGGCCAAG CGCTTCGTGG TGGTACGGCG GCCCTACGCC 
TACCTGTACA ACAGCGACAA GGACTCCGTG GAGAGGTTCG TGCTCAACCT GTCCACCGCG 
CAGGTGGAGT ACAGCGAGGA CCAGCAGGCC ATGCTCAAGA CGCCCAACAC GTTCGCCGTG 
TGCACGGAGC ACCGCGGCAT CCTGCTGCAA GCCAACAGCG ACAAGGACAT GCACGACTGG 
CTGTACGCCT TCAACCCGCT CCTGGCCGGG ACCATACGGT CCAAGCTCTC CAGAAGGCGG 
TCTGCCCAGA TGAGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409571.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265279.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

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CloneID	OCe297013
Clone ID Related Accession (Same CDS sequence)	XM_022409571.1
Accession Version	XM_022409571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCATCTCTGG CTGCAACAGC 
TACCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGG AGGACGACGT CTTTCCGGAG 
CACGCGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTCTACCT GAGCAACCTG CTGTACCCTG TCCCCCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG GGCTTCCTCC GCGTGGCCGT GCAGGCCACC 
TCAGCCGACG AAGAGGCCCC CGACTATGGC TCTGGCGTCC GACAGTCCGG CACGGCCAGA 
ATCTCCTTTG ACGACCAGCA TTTTGAAAAG TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG 
ATGTCCCGCT CGGGGACCTC CCAGGAAGAG CTGCGCATCG TGGAGGGTCA GGGCCAGGGT 
GCAGACACCG GGCCCTCGGC CGACGAGGTC AACAACAACA CCTGCTCAGC CGTGCCCCCG 
GAAGGCCTCC TCCTGGACAG CCCCGAGAAG GCCGCCACAG ATGGGCCTCT GGACGCCGCC 
CTGGACCACC TGCGCCTGGG CAGCACCTTC ACCTTCCGCG TGACTGTCCT GCAGGCGTCC 
AGCATCTCCG CCGAATACGC CGACATTTTC TGCCAGTTCA ACTTCATCCA CCGCCACGAC 
GAGGCCTTCT CCACGGAGCC CCTCAAGAAC ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC 
CACGTCCAGA ACATCGCAGT GGAGGTGACC AAGTCCTTCA TCGAGTACAT CAAGAGCCAG 
CCCATCGTGT TCGAGGTCTT CGGCCACTAC CAGCAGCACC CGTTCCCGCC CCTCTGCAAG 
GATGTGCTCA GCCCCCTGAG GCCCTCGCGC CGCCACTTCC CGAGGGTCAT GCCGCTGTCC 
AAGCCAGTGC CTGCCACCAA GCTCAGCACG CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC 
TGCAAGTACG ACCTGCTGGT CTACTTTGAG ATCTGTGAGC TGGAGGCCAA CGGCGATTAC 
ATTCCCGCAG TGGTGGACCA CCGTGGCGGG ATGCCGTGCA TGGGGACCTT CCTGCTGCAC 
CAGGGCATCC AGAGACGGAT CACCGTGACG CTGCTGCACG AGACGGGCAG CCACATCCGC 
TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT CGGATCCGAA ACACTCCCGA AACCGATGAG 
TCCCTGATCG ACCCCAACAT CTTGTCGCTC AACATCCTCT CCTCCGATTA TATCCACCCG 
GCCCAGGACG ACCGGCAGTT TCTTGATTCG GACATGCCTA GCGTTTCATT CGGAAATGAC 
ACTAGGACCT TCTACCAATT CGAGGCTGCG TGGGACAGCT CCATGCACAA CTCTCTTCTG 
CTCAACCGGG TCACCCCGTA CCGAGAGAAG ATCTACATGA CTCTCTCAGC TTACATTGAG 
ATGGAGAACT GCACTCAGCC GGCCGTCATC ACCAAGGACT TCTGCATGGT CTTCTACTCT 
CGGGACGCCA AGCTGCCCGC TTCCCGCTCC ATCCGCAACC TGTTTGGCAG CGGGAGCCTC 
CGGGCCTCGG AGAGCAACCG CGTGACGGGC GTGTACGAGC TCAGCCTGTG CCATGTGGCC 
GACGCCGGCA GCCCAGGGAT GCAGCGGCGG CGGCGGCGCG TGCTGGATAC ATCCGTGGCC 
TACGTGCGGG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GGAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTTGAGAA GACCAGACAC 
TACCTTCTGC TGCGGGAGAA GCTGGAGACC ACCCAGCGGC CCGGCCCTGA GGCGCCGTCC 
CCCGCCTCCA GCGAGGACTT GGGGTCCCAT GGCTCCTCCA GCCCCTCGTC CCCGCTCTCA 
GCTGAGGGCC GCCCGTCGCC TCTGGAGACT CCCAATGAGA GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GCCTCCTCAC GCACACGTTC AACCGAGAGT ACACCCACAG CCACGTCTGC 
ATCAGTGCCA GCGAGAGCAA GCTCTCCGAG ATGTCTGTCA CCCTGCTTCG GGACCCATCC 
ATGTCCCCGC TGGGGGCGGC CACCCTCACC CCCTCCTCCA CCTGCCCCTC TCTGGTCGAA 
GGGCGGTACG GAGCCGCTGA GCTGAGGACC CCCCAGCCCT GCTCCCGGCC CGCCAGCCCA 
GAGCCTGAGC CTGTGCCAGA GGCAGATTCC AAGAAGCTTC CCTCCCCTGC TCGGGCTGCG 
GAGGCCGACA AGGAGCCCCA GCGCCTGCTG GTTCCCGACA TCCAGGAGAT CCGGGTCAGC 
CCCATCGTGT CTAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTACG GCGGCCCTAC GCCTACCTGT ACAACAGCGA CAAGGACTCC 
GTGGAGAGGT TCGTGCTCAA CCTGTCCACC GCGCAGGTGG AGTACAGCGA GGACCAGCAG 
GCCATGCTCA AGACGCCCAA CACGTTCGCC GTGTGCACGG AGCACCGCGG CATCCTGCTG 
CAAGCCAACA GCGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC GCTCCTGGCC 
GGGACCATAC GGTCCAAGCT CTCCAGAAGG CGGTCTGCCC AGATGAGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265279.1
CDS	207..5600
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409571.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409571.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCATCTCTGG CTGCAACAGC 
TACCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGG AGGACGACGT CTTTCCGGAG 
CACGCGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTCTACCT GAGCAACCTG CTGTACCCTG TCCCCCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG GGCTTCCTCC GCGTGGCCGT GCAGGCCACC 
TCAGCCGACG AAGAGGCCCC CGACTATGGC TCTGGCGTCC GACAGTCCGG CACGGCCAGA 
ATCTCCTTTG ACGACCAGCA TTTTGAAAAG TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG 
ATGTCCCGCT CGGGGACCTC CCAGGAAGAG CTGCGCATCG TGGAGGGTCA GGGCCAGGGT 
GCAGACACCG GGCCCTCGGC CGACGAGGTC AACAACAACA CCTGCTCAGC CGTGCCCCCG 
GAAGGCCTCC TCCTGGACAG CCCCGAGAAG GCCGCCACAG ATGGGCCTCT GGACGCCGCC 
CTGGACCACC TGCGCCTGGG CAGCACCTTC ACCTTCCGCG TGACTGTCCT GCAGGCGTCC 
AGCATCTCCG CCGAATACGC CGACATTTTC TGCCAGTTCA ACTTCATCCA CCGCCACGAC 
GAGGCCTTCT CCACGGAGCC CCTCAAGAAC ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC 
CACGTCCAGA ACATCGCAGT GGAGGTGACC AAGTCCTTCA TCGAGTACAT CAAGAGCCAG 
CCCATCGTGT TCGAGGTCTT CGGCCACTAC CAGCAGCACC CGTTCCCGCC CCTCTGCAAG 
GATGTGCTCA GCCCCCTGAG GCCCTCGCGC CGCCACTTCC CGAGGGTCAT GCCGCTGTCC 
AAGCCAGTGC CTGCCACCAA GCTCAGCACG CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC 
TGCAAGTACG ACCTGCTGGT CTACTTTGAG ATCTGTGAGC TGGAGGCCAA CGGCGATTAC 
ATTCCCGCAG TGGTGGACCA CCGTGGCGGG ATGCCGTGCA TGGGGACCTT CCTGCTGCAC 
CAGGGCATCC AGAGACGGAT CACCGTGACG CTGCTGCACG AGACGGGCAG CCACATCCGC 
TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT CGGATCCGAA ACACTCCCGA AACCGATGAG 
TCCCTGATCG ACCCCAACAT CTTGTCGCTC AACATCCTCT CCTCCGATTA TATCCACCCG 
GCCCAGGACG ACCGGCAGTT TCTTGATTCG GACATGCCTA GCGTTTCATT CGGAAATGAC 
ACTAGGACCT TCTACCAATT CGAGGCTGCG TGGGACAGCT CCATGCACAA CTCTCTTCTG 
CTCAACCGGG TCACCCCGTA CCGAGAGAAG ATCTACATGA CTCTCTCAGC TTACATTGAG 
ATGGAGAACT GCACTCAGCC GGCCGTCATC ACCAAGGACT TCTGCATGGT CTTCTACTCT 
CGGGACGCCA AGCTGCCCGC TTCCCGCTCC ATCCGCAACC TGTTTGGCAG CGGGAGCCTC 
CGGGCCTCGG AGAGCAACCG CGTGACGGGC GTGTACGAGC TCAGCCTGTG CCATGTGGCC 
GACGCCGGCA GCCCAGGGAT GCAGCGGCGG CGGCGGCGCG TGCTGGATAC ATCCGTGGCC 
TACGTGCGGG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GGAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTTGAGAA GACCAGACAC 
TACCTTCTGC TGCGGGAGAA GCTGGAGACC ACCCAGCGGC CCGGCCCTGA GGCGCCGTCC 
CCCGCCTCCA GCGAGGACTT GGGGTCCCAT GGCTCCTCCA GCCCCTCGTC CCCGCTCTCA 
GCTGAGGGCC GCCCGTCGCC TCTGGAGACT CCCAATGAGA GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GCCTCCTCAC GCACACGTTC AACCGAGAGT ACACCCACAG CCACGTCTGC 
ATCAGTGCCA GCGAGAGCAA GCTCTCCGAG ATGTCTGTCA CCCTGCTTCG GGACCCATCC 
ATGTCCCCGC TGGGGGCGGC CACCCTCACC CCCTCCTCCA CCTGCCCCTC TCTGGTCGAA 
GGGCGGTACG GAGCCGCTGA GCTGAGGACC CCCCAGCCCT GCTCCCGGCC CGCCAGCCCA 
GAGCCTGAGC CTGTGCCAGA GGCAGATTCC AAGAAGCTTC CCTCCCCTGC TCGGGCTGCG 
GAGGCCGACA AGGAGCCCCA GCGCCTGCTG GTTCCCGACA TCCAGGAGAT CCGGGTCAGC 
CCCATCGTGT CTAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTACG GCGGCCCTAC GCCTACCTGT ACAACAGCGA CAAGGACTCC 
GTGGAGAGGT TCGTGCTCAA CCTGTCCACC GCGCAGGTGG AGTACAGCGA GGACCAGCAG 
GCCATGCTCA AGACGCCCAA CACGTTCGCC GTGTGCACGG AGCACCGCGG CATCCTGCTG 
CAAGCCAACA GCGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC GCTCCTGGCC 
GGGACCATAC GGTCCAAGCT CTCCAGAAGG CGGTCTGCCC AGATGAGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409572.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265280.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

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CloneID	OCe297014
Clone ID Related Accession (Same CDS sequence)	XM_022409572.1
Accession Version	XM_022409572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GCAGTTTCTT 
GATTCGGACA TGCCTAGCGT TTCATTCGGA AATGACACTA GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265280.1
CDS	207..5576
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPSVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409572.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409572.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GCAGTTTCTT 
GATTCGGACA TGCCTAGCGT TTCATTCGGA AATGACACTA GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_022409576.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265284.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X16, mRNA.

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CloneID	OCe297018
Clone ID Related Accession (Same CDS sequence)	XM_022409576.1
Accession Version	XM_022409576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GCGTTTCATT CGGAAATGAC 
ACTAGGACCT TCTACCAATT CGAGGCTGCG TGGGACAGCT CCATGCACAA CTCTCTTCTG 
CTCAACCGGG TCACCCCGTA CCGAGAGAAG ATCTACATGA CTCTCTCAGC TTACATTGAG 
ATGGAGAACT GCACTCAGCC GGCCGTCATC ACCAAGGACT TCTGCATGGT CTTCTACTCT 
CGGGACGCCA AGCTGCCCGC TTCCCGCTCC ATCCGCAACC TGTTTGGCAG CGGGAGCCTC 
CGGGCCTCGG AGAGCAACCG CGTGACGGGC GTGTACGAGC TCAGCCTGTG CCATGTGGCC 
GACGCCGGCA GCCCAGGGAT GCAGCGGCGG CGGCGGCGCG TGCTGGATAC ATCCGTGGCC 
TACGTGCGGG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GGAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTTGAGAA GACCAGACAC 
TACCTTCTGC TGCGGGAGAA GCTGGAGACC ACCCAGCGGC CCGGCCCTGA GGCGCCGTCC 
CCCGCCTCCA GCGAGGACTT GGGGTCCCAT GGCTCCTCCA GCCCCTCGTC CCCGCTCTCA 
GCTGAGGGCC GCCCGTCGCC TCTGGAGACT CCCAATGAGA GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GCCTCCTCAC GCACACGTTC AACCGAGAGT ACACCCACAG CCACGTCTGC 
ATCAGTGCCA GCGAGAGCAA GCTCTCCGAG ATGTCTGTCA CCCTGCTTCG GGACCCATCC 
ATGTCCCCGC TGGGGGCGGC CACCCTCACC CCCTCCTCCA CCTGCCCCTC TCTGGTCGAA 
GGGCGGTACG GAGCCGCTGA GCTGAGGACC CCCCAGCCCT GCTCCCGGCC CGCCAGCCCA 
GAGCCTGAGC CTGTGCCAGA GGCAGATTCC AAGAAGCTTC CCTCCCCTGC TCGGGCTGCG 
GAGGCCGACA AGGAGCCCCA GCGCCTGCTG GTTCCCGACA TCCAGGAGAT CCGGGTCAGC 
CCCATCGTGT CTAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTACG GCGGCCCTAC GCCTACCTGT ACAACAGCGA CAAGGACTCC 
GTGGAGAGGT TCGTGCTCAA CCTGTCCACC GCGCAGGTGG AGTACAGCGA GGACCAGCAG 
GCCATGCTCA AGACGCCCAA CACGTTCGCC GTGTGCACGG AGCACCGCGG CATCCTGCTG 
CAAGCCAACA GCGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC GCTCCTGGCC 
GGGACCATAC GGTCCAAGCT CTCCAGAAGG CGGTCTGCCC AGATGAGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265284.1
CDS	13..5406
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_022409576.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409576.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GCGTTTCATT CGGAAATGAC 
ACTAGGACCT TCTACCAATT CGAGGCTGCG TGGGACAGCT CCATGCACAA CTCTCTTCTG 
CTCAACCGGG TCACCCCGTA CCGAGAGAAG ATCTACATGA CTCTCTCAGC TTACATTGAG 
ATGGAGAACT GCACTCAGCC GGCCGTCATC ACCAAGGACT TCTGCATGGT CTTCTACTCT 
CGGGACGCCA AGCTGCCCGC TTCCCGCTCC ATCCGCAACC TGTTTGGCAG CGGGAGCCTC 
CGGGCCTCGG AGAGCAACCG CGTGACGGGC GTGTACGAGC TCAGCCTGTG CCATGTGGCC 
GACGCCGGCA GCCCAGGGAT GCAGCGGCGG CGGCGGCGCG TGCTGGATAC ATCCGTGGCC 
TACGTGCGGG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GGAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTTGAGAA GACCAGACAC 
TACCTTCTGC TGCGGGAGAA GCTGGAGACC ACCCAGCGGC CCGGCCCTGA GGCGCCGTCC 
CCCGCCTCCA GCGAGGACTT GGGGTCCCAT GGCTCCTCCA GCCCCTCGTC CCCGCTCTCA 
GCTGAGGGCC GCCCGTCGCC TCTGGAGACT CCCAATGAGA GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GCCTCCTCAC GCACACGTTC AACCGAGAGT ACACCCACAG CCACGTCTGC 
ATCAGTGCCA GCGAGAGCAA GCTCTCCGAG ATGTCTGTCA CCCTGCTTCG GGACCCATCC 
ATGTCCCCGC TGGGGGCGGC CACCCTCACC CCCTCCTCCA CCTGCCCCTC TCTGGTCGAA 
GGGCGGTACG GAGCCGCTGA GCTGAGGACC CCCCAGCCCT GCTCCCGGCC CGCCAGCCCA 
GAGCCTGAGC CTGTGCCAGA GGCAGATTCC AAGAAGCTTC CCTCCCCTGC TCGGGCTGCG 
GAGGCCGACA AGGAGCCCCA GCGCCTGCTG GTTCCCGACA TCCAGGAGAT CCGGGTCAGC 
CCCATCGTGT CTAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTACG GCGGCCCTAC GCCTACCTGT ACAACAGCGA CAAGGACTCC 
GTGGAGAGGT TCGTGCTCAA CCTGTCCACC GCGCAGGTGG AGTACAGCGA GGACCAGCAG 
GCCATGCTCA AGACGCCCAA CACGTTCGCC GTGTGCACGG AGCACCGCGG CATCCTGCTG 
CAAGCCAACA GCGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC GCTCCTGGCC 
GGGACCATAC GGTCCAAGCT CTCCAGAAGG CGGTCTGCCC AGATGAGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107680.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963155.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

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CloneID	OCe43164
Clone ID Related Accession (Same CDS sequence)	XM_014107680.1 , XM_014107680.2
Accession Version	XM_014107680.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963155.1
CDS	194..5668
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107680.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107680.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107680.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963155.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

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CloneID	OCe43164
Clone ID Related Accession (Same CDS sequence)	XM_014107680.1 , XM_014107680.2
Accession Version	XM_014107680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963155.1
CDS	207..5681
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107680.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107680.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
CAGTTTCTTG ATTCGGACAT GCCTAGGACC TTCTACCAAT TCGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCTCTTCT GCTCAACCGG GTCACCCCGT ACCGAGAGAA GATCTACATG 
ACTCTCTCAG CTTACATTGA GATGGAGAAC TGCACTCAGC CGGCCGTCAT CACCAAGGAC 
TTCTGCATGG TCTTCTACTC TCGGGACGCC AAGCTGCCCG CTTCCCGCTC CATCCGCAAC 
CTGTTTGGCA GCGGGAGCCT CCGGGCCTCG GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTCAGCCTGT GCCATGTGGC CGACGCCGGC AGCCCAGGGA TGCAGCGGCG GCGGCGGCGC 
GTGCTGGATA CATCCGTGGC CTACGTGCGG GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGGAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTTGAGA AGACCAGACA CTACCTTCTG CTGCGGGAGA AGCTGGAGAC CACCCAGCGG 
CCCGGCCCTG AGGCGCCGTC CCCCGCCTCC AGCGAGGACT TGGGGTCCCA TGGCTCCTCC 
AGCCCCTCGT CCCCGCTCTC AGCTGAGGGC CGCCCGTCGC CTCTGGAGAC TCCCAATGAG 
AGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGCCTCCTCA CGCACACGTT CAACCGAGAG 
TACACCCACA GCCACGTCTG CATCAGTGCC AGCGAGAGCA AGCTCTCCGA GATGTCTGTC 
ACCCTGCTTC GGGACCCATC CATGTCCCCG CTGGGGGCGG CCACCCTCAC CCCCTCCTCC 
ACCTGCCCCT CTCTGGTCGA AGGGCGGTAC GGAGCCGCTG AGCTGAGGAC CCCCCAGCCC 
TGCTCCCGGC CCGCCAGCCC AGAGCCTGAG CCTGTGCCAG AGGCAGATTC CAAGAAGCTT 
CCCTCCCCTG CTCGGGCTGC GGAGGCCGAC AAGGAGCCCC AGCGCCTGCT GGTTCCCGAC 
ATCCAGGAGA TCCGGGTCAG CCCCATCGTG TCTAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTAC GGCGGCCCTA CGCCTACCTG 
TACAACAGCG ACAAGGACTC CGTGGAGAGG TTCGTGCTCA ACCTGTCCAC CGCGCAGGTG 
GAGTACAGCG AGGACCAGCA GGCCATGCTC AAGACGCCCA ACACGTTCGC CGTGTGCACG 
GAGCACCGCG GCATCCTGCT GCAAGCCAAC AGCGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CGCTCCTGGC CGGGACCATA CGGTCCAAGC TCTCCAGAAG GCGGTCTGCC 
CAGATGAGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107681.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963156.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

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CloneID	OCe43165
Clone ID Related Accession (Same CDS sequence)	XM_014107681.1 , XM_014107681.2
Accession Version	XM_014107681.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGC 
GTTTCATTCG GAAATGACAC TAGGACCTTC TACCAATTCG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CTCTTCTGCT CAACCGGGTC ACCCCGTACC GAGAGAAGAT CTACATGACT 
CTCTCAGCTT ACATTGAGAT GGAGAACTGC ACTCAGCCGG CCGTCATCAC CAAGGACTTC 
TGCATGGTCT TCTACTCTCG GGACGCCAAG CTGCCCGCTT CCCGCTCCAT CCGCAACCTG 
TTTGGCAGCG GGAGCCTCCG GGCCTCGGAG AGCAACCGCG TGACGGGCGT GTACGAGCTC 
AGCCTGTGCC ATGTGGCCGA CGCCGGCAGC CCAGGGATGC AGCGGCGGCG GCGGCGCGTG 
CTGGATACAT CCGTGGCCTA CGTGCGGGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGG 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTTGAGAAGA CCAGACACTA CCTTCTGCTG CGGGAGAAGC TGGAGACCAC CCAGCGGCCC 
GGCCCTGAGG CGCCGTCCCC CGCCTCCAGC GAGGACTTGG GGTCCCATGG CTCCTCCAGC 
CCCTCGTCCC CGCTCTCAGC TGAGGGCCGC CCGTCGCCTC TGGAGACTCC CAATGAGAGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGC CTCCTCACGC ACACGTTCAA CCGAGAGTAC 
ACCCACAGCC ACGTCTGCAT CAGTGCCAGC GAGAGCAAGC TCTCCGAGAT GTCTGTCACC 
CTGCTTCGGG ACCCATCCAT GTCCCCGCTG GGGGCGGCCA CCCTCACCCC CTCCTCCACC 
TGCCCCTCTC TGGTCGAAGG GCGGTACGGA GCCGCTGAGC TGAGGACCCC CCAGCCCTGC 
TCCCGGCCCG CCAGCCCAGA GCCTGAGCCT GTGCCAGAGG CAGATTCCAA GAAGCTTCCC 
TCCCCTGCTC GGGCTGCGGA GGCCGACAAG GAGCCCCAGC GCCTGCTGGT TCCCGACATC 
CAGGAGATCC GGGTCAGCCC CATCGTGTCT AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTACGGC GGCCCTACGC CTACCTGTAC 
AACAGCGACA AGGACTCCGT GGAGAGGTTC GTGCTCAACC TGTCCACCGC GCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC CATGCTCAAG ACGCCCAACA CGTTCGCCGT GTGCACGGAG 
CACCGCGGCA TCCTGCTGCA AGCCAACAGC GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCGC TCCTGGCCGG GACCATACGG TCCAAGCTCT CCAGAAGGCG GTCTGCCCAG 
ATGAGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963156.1
CDS	195..5666
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107681.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107681.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGC 
GTTTCATTCG GAAATGACAC TAGGACCTTC TACCAATTCG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CTCTTCTGCT CAACCGGGTC ACCCCGTACC GAGAGAAGAT CTACATGACT 
CTCTCAGCTT ACATTGAGAT GGAGAACTGC ACTCAGCCGG CCGTCATCAC CAAGGACTTC 
TGCATGGTCT TCTACTCTCG GGACGCCAAG CTGCCCGCTT CCCGCTCCAT CCGCAACCTG 
TTTGGCAGCG GGAGCCTCCG GGCCTCGGAG AGCAACCGCG TGACGGGCGT GTACGAGCTC 
AGCCTGTGCC ATGTGGCCGA CGCCGGCAGC CCAGGGATGC AGCGGCGGCG GCGGCGCGTG 
CTGGATACAT CCGTGGCCTA CGTGCGGGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGG 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTTGAGAAGA CCAGACACTA CCTTCTGCTG CGGGAGAAGC TGGAGACCAC CCAGCGGCCC 
GGCCCTGAGG CGCCGTCCCC CGCCTCCAGC GAGGACTTGG GGTCCCATGG CTCCTCCAGC 
CCCTCGTCCC CGCTCTCAGC TGAGGGCCGC CCGTCGCCTC TGGAGACTCC CAATGAGAGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGC CTCCTCACGC ACACGTTCAA CCGAGAGTAC 
ACCCACAGCC ACGTCTGCAT CAGTGCCAGC GAGAGCAAGC TCTCCGAGAT GTCTGTCACC 
CTGCTTCGGG ACCCATCCAT GTCCCCGCTG GGGGCGGCCA CCCTCACCCC CTCCTCCACC 
TGCCCCTCTC TGGTCGAAGG GCGGTACGGA GCCGCTGAGC TGAGGACCCC CCAGCCCTGC 
TCCCGGCCCG CCAGCCCAGA GCCTGAGCCT GTGCCAGAGG CAGATTCCAA GAAGCTTCCC 
TCCCCTGCTC GGGCTGCGGA GGCCGACAAG GAGCCCCAGC GCCTGCTGGT TCCCGACATC 
CAGGAGATCC GGGTCAGCCC CATCGTGTCT AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTACGGC GGCCCTACGC CTACCTGTAC 
AACAGCGACA AGGACTCCGT GGAGAGGTTC GTGCTCAACC TGTCCACCGC GCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC CATGCTCAAG ACGCCCAACA CGTTCGCCGT GTGCACGGAG 
CACCGCGGCA TCCTGCTGCA AGCCAACAGC GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCGC TCCTGGCCGG GACCATACGG TCCAAGCTCT CCAGAAGGCG GTCTGCCCAG 
ATGAGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107681.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963156.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

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CloneID	OCe43165
Clone ID Related Accession (Same CDS sequence)	XM_014107681.1 , XM_014107681.2
Accession Version	XM_014107681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGC 
GTTTCATTCG GAAATGACAC TAGGACCTTC TACCAATTCG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CTCTTCTGCT CAACCGGGTC ACCCCGTACC GAGAGAAGAT CTACATGACT 
CTCTCAGCTT ACATTGAGAT GGAGAACTGC ACTCAGCCGG CCGTCATCAC CAAGGACTTC 
TGCATGGTCT TCTACTCTCG GGACGCCAAG CTGCCCGCTT CCCGCTCCAT CCGCAACCTG 
TTTGGCAGCG GGAGCCTCCG GGCCTCGGAG AGCAACCGCG TGACGGGCGT GTACGAGCTC 
AGCCTGTGCC ATGTGGCCGA CGCCGGCAGC CCAGGGATGC AGCGGCGGCG GCGGCGCGTG 
CTGGATACAT CCGTGGCCTA CGTGCGGGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGG 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTTGAGAAGA CCAGACACTA CCTTCTGCTG CGGGAGAAGC TGGAGACCAC CCAGCGGCCC 
GGCCCTGAGG CGCCGTCCCC CGCCTCCAGC GAGGACTTGG GGTCCCATGG CTCCTCCAGC 
CCCTCGTCCC CGCTCTCAGC TGAGGGCCGC CCGTCGCCTC TGGAGACTCC CAATGAGAGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGC CTCCTCACGC ACACGTTCAA CCGAGAGTAC 
ACCCACAGCC ACGTCTGCAT CAGTGCCAGC GAGAGCAAGC TCTCCGAGAT GTCTGTCACC 
CTGCTTCGGG ACCCATCCAT GTCCCCGCTG GGGGCGGCCA CCCTCACCCC CTCCTCCACC 
TGCCCCTCTC TGGTCGAAGG GCGGTACGGA GCCGCTGAGC TGAGGACCCC CCAGCCCTGC 
TCCCGGCCCG CCAGCCCAGA GCCTGAGCCT GTGCCAGAGG CAGATTCCAA GAAGCTTCCC 
TCCCCTGCTC GGGCTGCGGA GGCCGACAAG GAGCCCCAGC GCCTGCTGGT TCCCGACATC 
CAGGAGATCC GGGTCAGCCC CATCGTGTCT AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTACGGC GGCCCTACGC CTACCTGTAC 
AACAGCGACA AGGACTCCGT GGAGAGGTTC GTGCTCAACC TGTCCACCGC GCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC CATGCTCAAG ACGCCCAACA CGTTCGCCGT GTGCACGGAG 
CACCGCGGCA TCCTGCTGCA AGCCAACAGC GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCGC TCCTGGCCGG GACCATACGG TCCAAGCTCT CCAGAAGGCG GTCTGCCCAG 
ATGAGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963156.1
CDS	207..5678
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRVSFGNDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107681.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107681.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGC 
GTTTCATTCG GAAATGACAC TAGGACCTTC TACCAATTCG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CTCTTCTGCT CAACCGGGTC ACCCCGTACC GAGAGAAGAT CTACATGACT 
CTCTCAGCTT ACATTGAGAT GGAGAACTGC ACTCAGCCGG CCGTCATCAC CAAGGACTTC 
TGCATGGTCT TCTACTCTCG GGACGCCAAG CTGCCCGCTT CCCGCTCCAT CCGCAACCTG 
TTTGGCAGCG GGAGCCTCCG GGCCTCGGAG AGCAACCGCG TGACGGGCGT GTACGAGCTC 
AGCCTGTGCC ATGTGGCCGA CGCCGGCAGC CCAGGGATGC AGCGGCGGCG GCGGCGCGTG 
CTGGATACAT CCGTGGCCTA CGTGCGGGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGG 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTTGAGAAGA CCAGACACTA CCTTCTGCTG CGGGAGAAGC TGGAGACCAC CCAGCGGCCC 
GGCCCTGAGG CGCCGTCCCC CGCCTCCAGC GAGGACTTGG GGTCCCATGG CTCCTCCAGC 
CCCTCGTCCC CGCTCTCAGC TGAGGGCCGC CCGTCGCCTC TGGAGACTCC CAATGAGAGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGC CTCCTCACGC ACACGTTCAA CCGAGAGTAC 
ACCCACAGCC ACGTCTGCAT CAGTGCCAGC GAGAGCAAGC TCTCCGAGAT GTCTGTCACC 
CTGCTTCGGG ACCCATCCAT GTCCCCGCTG GGGGCGGCCA CCCTCACCCC CTCCTCCACC 
TGCCCCTCTC TGGTCGAAGG GCGGTACGGA GCCGCTGAGC TGAGGACCCC CCAGCCCTGC 
TCCCGGCCCG CCAGCCCAGA GCCTGAGCCT GTGCCAGAGG CAGATTCCAA GAAGCTTCCC 
TCCCCTGCTC GGGCTGCGGA GGCCGACAAG GAGCCCCAGC GCCTGCTGGT TCCCGACATC 
CAGGAGATCC GGGTCAGCCC CATCGTGTCT AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTACGGC GGCCCTACGC CTACCTGTAC 
AACAGCGACA AGGACTCCGT GGAGAGGTTC GTGCTCAACC TGTCCACCGC GCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC CATGCTCAAG ACGCCCAACA CGTTCGCCGT GTGCACGGAG 
CACCGCGGCA TCCTGCTGCA AGCCAACAGC GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCGC TCCTGGCCGG GACCATACGG TCCAAGCTCT CCAGAAGGCG GTCTGCCCAG 
ATGAGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107682.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963157.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

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CloneID	OCe43166
Clone ID Related Accession (Same CDS sequence)	XM_014107682.1
Accession Version	XM_014107682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG CTACCCTCTT CTCAACACAT GCATGAGCGA GCGCATGGCT 
GCTCTCACCC CCTCCCCCAC CTTCTCGAGC CCCGACTCCG ACGCCACCGA GCCTGCCGAG 
GAGCAGAGCG TGGGGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGACCTG 
GAGGACGACG TCTTTCCGGA GCACGCGCTG TGCGACGGCC GGGACCCGTT TTACGACCGG 
CCCCCCCTGT TCAGTTTAGT AGGAAGGGCC TTCGTCTACC TGAGCAACCT GCTGTACCCT 
GTCCCCCTGG TGCACCGCGT GGCCGTCGTC AGCGAGAAGG GAGAGGTGAA GGGCTTCCTC 
CGCGTGGCCG TGCAGGCCAC CTCAGCCGAC GAAGAGGCCC CCGACTATGG CTCTGGCGTC 
CGACAGTCCG GCACGGCCAG AATCTCCTTT GACGACCAGC ATTTTGAAAA GTTCCAGTCT 
GAGTCCTGCC CTGTGGTGGG GATGTCCCGC TCGGGGACCT CCCAGGAAGA GCTGCGCATC 
GTGGAGGGTC AGGGCCAGGG TGCAGACACC GGGCCCTCGG CCGACGAGGT CAACAACAAC 
ACCTGCTCAG CCGTGCCCCC GGAAGGCCTC CTCCTGGACA GCCCCGAGAA GGCCGCCACA 
GATGGGCCTC TGGACGCCGC CCTGGACCAC CTGCGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACTGTCC TGCAGGCGTC CAGCATCTCC GCCGAATACG CCGACATTTT CTGCCAGTTC 
AACTTCATCC ACCGCCACGA CGAGGCCTTC TCCACGGAGC CCCTCAAGAA CACAGGGAGG 
GGCCCCCCGC TTGGCTTCTA CCACGTCCAG AACATCGCAG TGGAGGTGAC CAAGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCATCGTG TTCGAGGTCT TCGGCCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGATGTGCTC AGCCCCCTGA GGCCCTCGCG CCGCCACTTC 
CCGAGGGTCA TGCCGCTGTC CAAGCCAGTG CCTGCCACCA AGCTCAGCAC GCTAGCGCGA 
CCCTGCCCTG GGCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTTGA GATCTGTGAG 
CTGGAGGCCA ACGGCGATTA CATTCCCGCA GTGGTGGACC ACCGTGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTGCA CCAGGGCATC CAGAGACGGA TCACCGTGAC GCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTTCGAGAGC TGGTCGTGGG TCGGATCCGA 
AACACTCCCG AAACCGATGA GTCCCTGATC GACCCCAACA TCTTGTCGCT CAACATCCTC 
TCCTCCGATT ATATCCACCC GGCCCAGGAC GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGGACCTTCT ACCAATTCGA GGCTGCGTGG GACAGCTCCA TGCACAACTC TCTTCTGCTC 
AACCGGGTCA CCCCGTACCG AGAGAAGATC TACATGACTC TCTCAGCTTA CATTGAGATG 
GAGAACTGCA CTCAGCCGGC CGTCATCACC AAGGACTTCT GCATGGTCTT CTACTCTCGG 
GACGCCAAGC TGCCCGCTTC CCGCTCCATC CGCAACCTGT TTGGCAGCGG GAGCCTCCGG 
GCCTCGGAGA GCAACCGCGT GACGGGCGTG TACGAGCTCA GCCTGTGCCA TGTGGCCGAC 
GCCGGCAGCC CAGGGATGCA GCGGCGGCGG CGGCGCGTGC TGGATACATC CGTGGCCTAC 
GTGCGGGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGGA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TTGAGAAGAC CAGACACTAC 
CTTCTGCTGC GGGAGAAGCT GGAGACCACC CAGCGGCCCG GCCCTGAGGC GCCGTCCCCC 
GCCTCCAGCG AGGACTTGGG GTCCCATGGC TCCTCCAGCC CCTCGTCCCC GCTCTCAGCT 
GAGGGCCGCC CGTCGCCTCT GGAGACTCCC AATGAGAGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGCC TCCTCACGCA CACGTTCAAC CGAGAGTACA CCCACAGCCA CGTCTGCATC 
AGTGCCAGCG AGAGCAAGCT CTCCGAGATG TCTGTCACCC TGCTTCGGGA CCCATCCATG 
TCCCCGCTGG GGGCGGCCAC CCTCACCCCC TCCTCCACCT GCCCCTCTCT GGTCGAAGGG 
CGGTACGGAG CCGCTGAGCT GAGGACCCCC CAGCCCTGCT CCCGGCCCGC CAGCCCAGAG 
CCTGAGCCTG TGCCAGAGGC AGATTCCAAG AAGCTTCCCT CCCCTGCTCG GGCTGCGGAG 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTT CCCGACATCC AGGAGATCCG GGTCAGCCCC 
ATCGTGTCTA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTACGGCG GCCCTACGCC TACCTGTACA ACAGCGACAA GGACTCCGTG 
GAGAGGTTCG TGCTCAACCT GTCCACCGCG CAGGTGGAGT ACAGCGAGGA CCAGCAGGCC 
ATGCTCAAGA CGCCCAACAC GTTCGCCGTG TGCACGGAGC ACCGCGGCAT CCTGCTGCAA 
GCCAACAGCG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCGCT CCTGGCCGGG 
ACCATACGGT CCAAGCTCTC CAGAAGGCGG TCTGCCCAGA TGAGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963157.1
CDS	194..5644
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107682.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107682.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG CTACCCTCTT CTCAACACAT GCATGAGCGA GCGCATGGCT 
GCTCTCACCC CCTCCCCCAC CTTCTCGAGC CCCGACTCCG ACGCCACCGA GCCTGCCGAG 
GAGCAGAGCG TGGGGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGACCTG 
GAGGACGACG TCTTTCCGGA GCACGCGCTG TGCGACGGCC GGGACCCGTT TTACGACCGG 
CCCCCCCTGT TCAGTTTAGT AGGAAGGGCC TTCGTCTACC TGAGCAACCT GCTGTACCCT 
GTCCCCCTGG TGCACCGCGT GGCCGTCGTC AGCGAGAAGG GAGAGGTGAA GGGCTTCCTC 
CGCGTGGCCG TGCAGGCCAC CTCAGCCGAC GAAGAGGCCC CCGACTATGG CTCTGGCGTC 
CGACAGTCCG GCACGGCCAG AATCTCCTTT GACGACCAGC ATTTTGAAAA GTTCCAGTCT 
GAGTCCTGCC CTGTGGTGGG GATGTCCCGC TCGGGGACCT CCCAGGAAGA GCTGCGCATC 
GTGGAGGGTC AGGGCCAGGG TGCAGACACC GGGCCCTCGG CCGACGAGGT CAACAACAAC 
ACCTGCTCAG CCGTGCCCCC GGAAGGCCTC CTCCTGGACA GCCCCGAGAA GGCCGCCACA 
GATGGGCCTC TGGACGCCGC CCTGGACCAC CTGCGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACTGTCC TGCAGGCGTC CAGCATCTCC GCCGAATACG CCGACATTTT CTGCCAGTTC 
AACTTCATCC ACCGCCACGA CGAGGCCTTC TCCACGGAGC CCCTCAAGAA CACAGGGAGG 
GGCCCCCCGC TTGGCTTCTA CCACGTCCAG AACATCGCAG TGGAGGTGAC CAAGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCATCGTG TTCGAGGTCT TCGGCCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGATGTGCTC AGCCCCCTGA GGCCCTCGCG CCGCCACTTC 
CCGAGGGTCA TGCCGCTGTC CAAGCCAGTG CCTGCCACCA AGCTCAGCAC GCTAGCGCGA 
CCCTGCCCTG GGCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTTGA GATCTGTGAG 
CTGGAGGCCA ACGGCGATTA CATTCCCGCA GTGGTGGACC ACCGTGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTGCA CCAGGGCATC CAGAGACGGA TCACCGTGAC GCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTTCGAGAGC TGGTCGTGGG TCGGATCCGA 
AACACTCCCG AAACCGATGA GTCCCTGATC GACCCCAACA TCTTGTCGCT CAACATCCTC 
TCCTCCGATT ATATCCACCC GGCCCAGGAC GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGGACCTTCT ACCAATTCGA GGCTGCGTGG GACAGCTCCA TGCACAACTC TCTTCTGCTC 
AACCGGGTCA CCCCGTACCG AGAGAAGATC TACATGACTC TCTCAGCTTA CATTGAGATG 
GAGAACTGCA CTCAGCCGGC CGTCATCACC AAGGACTTCT GCATGGTCTT CTACTCTCGG 
GACGCCAAGC TGCCCGCTTC CCGCTCCATC CGCAACCTGT TTGGCAGCGG GAGCCTCCGG 
GCCTCGGAGA GCAACCGCGT GACGGGCGTG TACGAGCTCA GCCTGTGCCA TGTGGCCGAC 
GCCGGCAGCC CAGGGATGCA GCGGCGGCGG CGGCGCGTGC TGGATACATC CGTGGCCTAC 
GTGCGGGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGGA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TTGAGAAGAC CAGACACTAC 
CTTCTGCTGC GGGAGAAGCT GGAGACCACC CAGCGGCCCG GCCCTGAGGC GCCGTCCCCC 
GCCTCCAGCG AGGACTTGGG GTCCCATGGC TCCTCCAGCC CCTCGTCCCC GCTCTCAGCT 
GAGGGCCGCC CGTCGCCTCT GGAGACTCCC AATGAGAGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGCC TCCTCACGCA CACGTTCAAC CGAGAGTACA CCCACAGCCA CGTCTGCATC 
AGTGCCAGCG AGAGCAAGCT CTCCGAGATG TCTGTCACCC TGCTTCGGGA CCCATCCATG 
TCCCCGCTGG GGGCGGCCAC CCTCACCCCC TCCTCCACCT GCCCCTCTCT GGTCGAAGGG 
CGGTACGGAG CCGCTGAGCT GAGGACCCCC CAGCCCTGCT CCCGGCCCGC CAGCCCAGAG 
CCTGAGCCTG TGCCAGAGGC AGATTCCAAG AAGCTTCCCT CCCCTGCTCG GGCTGCGGAG 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTT CCCGACATCC AGGAGATCCG GGTCAGCCCC 
ATCGTGTCTA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTACGGCG GCCCTACGCC TACCTGTACA ACAGCGACAA GGACTCCGTG 
GAGAGGTTCG TGCTCAACCT GTCCACCGCG CAGGTGGAGT ACAGCGAGGA CCAGCAGGCC 
ATGCTCAAGA CGCCCAACAC GTTCGCCGTG TGCACGGAGC ACCGCGGCAT CCTGCTGCAA 
GCCAACAGCG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCGCT CCTGGCCGGG 
ACCATACGGT CCAAGCTCTC CAGAAGGCGG TCTGCCCAGA TGAGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107683.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963158.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

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CloneID	OCe43167
Clone ID Related Accession (Same CDS sequence)	XM_014107683.2 , XM_014107683.1
Accession Version	XM_014107683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
ACCTTCTACC AATTCGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCTCT TCTGCTCAAC 
CGGGTCACCC CGTACCGAGA GAAGATCTAC ATGACTCTCT CAGCTTACAT TGAGATGGAG 
AACTGCACTC AGCCGGCCGT CATCACCAAG GACTTCTGCA TGGTCTTCTA CTCTCGGGAC 
GCCAAGCTGC CCGCTTCCCG CTCCATCCGC AACCTGTTTG GCAGCGGGAG CCTCCGGGCC 
TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTCAGCC TGTGCCATGT GGCCGACGCC 
GGCAGCCCAG GGATGCAGCG GCGGCGGCGG CGCGTGCTGG ATACATCCGT GGCCTACGTG 
CGGGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGGAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTTG AGAAGACCAG ACACTACCTT 
CTGCTGCGGG AGAAGCTGGA GACCACCCAG CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC 
TCCAGCGAGG ACTTGGGGTC CCATGGCTCC TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG 
GGCCGCCCGT CGCCTCTGGA GACTCCCAAT GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGCCTCC TCACGCACAC GTTCAACCGA GAGTACACCC ACAGCCACGT CTGCATCAGT 
GCCAGCGAGA GCAAGCTCTC CGAGATGTCT GTCACCCTGC TTCGGGACCC ATCCATGTCC 
CCGCTGGGGG CGGCCACCCT CACCCCCTCC TCCACCTGCC CCTCTCTGGT CGAAGGGCGG 
TACGGAGCCG CTGAGCTGAG GACCCCCCAG CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT 
GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTTCCC GACATCCAGG AGATCCGGGT CAGCCCCATC 
GTGTCTAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TACGGCGGCC CTACGCCTAC CTGTACAACA GCGACAAGGA CTCCGTGGAG 
AGGTTCGTGC TCAACCTGTC CACCGCGCAG GTGGAGTACA GCGAGGACCA GCAGGCCATG 
CTCAAGACGC CCAACACGTT CGCCGTGTGC ACGGAGCACC GCGGCATCCT GCTGCAAGCC 
AACAGCGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCGCTCCT GGCCGGGACC 
ATACGGTCCA AGCTCTCCAG AAGGCGGTCT GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963158.1
CDS	207..5654
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107683.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107683.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
ACCTTCTACC AATTCGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCTCT TCTGCTCAAC 
CGGGTCACCC CGTACCGAGA GAAGATCTAC ATGACTCTCT CAGCTTACAT TGAGATGGAG 
AACTGCACTC AGCCGGCCGT CATCACCAAG GACTTCTGCA TGGTCTTCTA CTCTCGGGAC 
GCCAAGCTGC CCGCTTCCCG CTCCATCCGC AACCTGTTTG GCAGCGGGAG CCTCCGGGCC 
TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTCAGCC TGTGCCATGT GGCCGACGCC 
GGCAGCCCAG GGATGCAGCG GCGGCGGCGG CGCGTGCTGG ATACATCCGT GGCCTACGTG 
CGGGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGGAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTTG AGAAGACCAG ACACTACCTT 
CTGCTGCGGG AGAAGCTGGA GACCACCCAG CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC 
TCCAGCGAGG ACTTGGGGTC CCATGGCTCC TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG 
GGCCGCCCGT CGCCTCTGGA GACTCCCAAT GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGCCTCC TCACGCACAC GTTCAACCGA GAGTACACCC ACAGCCACGT CTGCATCAGT 
GCCAGCGAGA GCAAGCTCTC CGAGATGTCT GTCACCCTGC TTCGGGACCC ATCCATGTCC 
CCGCTGGGGG CGGCCACCCT CACCCCCTCC TCCACCTGCC CCTCTCTGGT CGAAGGGCGG 
TACGGAGCCG CTGAGCTGAG GACCCCCCAG CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT 
GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTTCCC GACATCCAGG AGATCCGGGT CAGCCCCATC 
GTGTCTAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TACGGCGGCC CTACGCCTAC CTGTACAACA GCGACAAGGA CTCCGTGGAG 
AGGTTCGTGC TCAACCTGTC CACCGCGCAG GTGGAGTACA GCGAGGACCA GCAGGCCATG 
CTCAAGACGC CCAACACGTT CGCCGTGTGC ACGGAGCACC GCGGCATCCT GCTGCAAGCC 
AACAGCGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCGCTCCT GGCCGGGACC 
ATACGGTCCA AGCTCTCCAG AAGGCGGTCT GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107683.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963158.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

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CloneID	OCe43167
Clone ID Related Accession (Same CDS sequence)	XM_014107683.2 , XM_014107683.1
Accession Version	XM_014107683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
ACCTTCTACC AATTCGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCTCT TCTGCTCAAC 
CGGGTCACCC CGTACCGAGA GAAGATCTAC ATGACTCTCT CAGCTTACAT TGAGATGGAG 
AACTGCACTC AGCCGGCCGT CATCACCAAG GACTTCTGCA TGGTCTTCTA CTCTCGGGAC 
GCCAAGCTGC CCGCTTCCCG CTCCATCCGC AACCTGTTTG GCAGCGGGAG CCTCCGGGCC 
TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTCAGCC TGTGCCATGT GGCCGACGCC 
GGCAGCCCAG GGATGCAGCG GCGGCGGCGG CGCGTGCTGG ATACATCCGT GGCCTACGTG 
CGGGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGGAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTTG AGAAGACCAG ACACTACCTT 
CTGCTGCGGG AGAAGCTGGA GACCACCCAG CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC 
TCCAGCGAGG ACTTGGGGTC CCATGGCTCC TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG 
GGCCGCCCGT CGCCTCTGGA GACTCCCAAT GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGCCTCC TCACGCACAC GTTCAACCGA GAGTACACCC ACAGCCACGT CTGCATCAGT 
GCCAGCGAGA GCAAGCTCTC CGAGATGTCT GTCACCCTGC TTCGGGACCC ATCCATGTCC 
CCGCTGGGGG CGGCCACCCT CACCCCCTCC TCCACCTGCC CCTCTCTGGT CGAAGGGCGG 
TACGGAGCCG CTGAGCTGAG GACCCCCCAG CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT 
GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTTCCC GACATCCAGG AGATCCGGGT CAGCCCCATC 
GTGTCTAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TACGGCGGCC CTACGCCTAC CTGTACAACA GCGACAAGGA CTCCGTGGAG 
AGGTTCGTGC TCAACCTGTC CACCGCGCAG GTGGAGTACA GCGAGGACCA GCAGGCCATG 
CTCAAGACGC CCAACACGTT CGCCGTGTGC ACGGAGCACC GCGGCATCCT GCTGCAAGCC 
AACAGCGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCGCTCCT GGCCGGGACC 
ATACGGTCCA AGCTCTCCAG AAGGCGGTCT GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963158.1
CDS	194..5641
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKYVSDLENNNRNRGGAELSPAPDNLSTVTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107683.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107683.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATACG TGTCCGACCT TGAGAACAAT AACCGTAACC GTGGCGGGGC GGAGCTGAGT 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AACGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCGCTCTCGG CCCTGTCCAG CCGCGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACGGAGAA GATCATAGCC 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAGGCCCTGC TGGCCGAGAT GGGCGTGGCC ATGAGGGAGG ATGGCGGCAC GCTGGGGGTG 
TTTTCTCCCA AAAAGACACC ACATCTTGTC AACCTGAATG AGGACCCCTT GATGTCCGAG 
TGTCTCCTGT ACTACATCAA GGATGGCATC ACCAGAGTGG GCCGGGAGGA TGCGGAGCGG 
CGGCAGGACA TCGTCCTGAG TGGGCACTTC ATCAAGGAGG AGCACTGCGT CTTCCGGAGT 
GATTCCCGAG GGGGCAGTGA AGCTGTGGTG ACCCTGGAGC CCTGCGAGGG CGCAGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCT AGCATCCTGC GGTCAGGCAA CCGCATCATC 
ATGGGAAAGA GCCACGTGTT CCGGTTCAAC CACCCCGAGC AGGCGAGGCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCCGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGTGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGCGA GCGGGAGGAG GCCACGTACC TGCTGGAGCA GCAGAGGCTG 
GACTACGAGA GCAAGCTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTACCCTGAG 
GTGAACGAGG AGGAGGAAGA GCCGGAGGAC GAAGTCCAGT GGACGGAGAG GGAGTGTGAG 
CTGGCGCTCT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACGTCGCT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAA GCCAACGCCA TTAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACC GACACGCTCT ACTCCCCGCT GCCGCCGGAC 
CTGCTGCCCC CAGAGGCCGC CAAAGACCGG GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAGCTC 
AGGCAGCGCC TGGACCTGAT GCGGGAGATG TACGACCGGG CGGCAGAGGT GCCCTCCAGT 
GTCATCGAGG ACTGTGACAA CGTGGTGACG GGCGGAGACC CCTTCTACGA CCGCTTTCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCATC TCTGGCTGCA ACAGCTACCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGGAGGAC GACGTCTTTC CGGAGCACGC GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTC 
TACCTGAGCA ACCTGCTGTA CCCTGTCCCC CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGAGAGG TGAAGGGCTT CCTCCGCGTG GCCGTGCAGG CCACCTCAGC CGACGAAGAG 
GCCCCCGACT ATGGCTCTGG CGTCCGACAG TCCGGCACGG CCAGAATCTC CTTTGACGAC 
CAGCATTTTG AAAAGTTCCA GTCTGAGTCC TGCCCTGTGG TGGGGATGTC CCGCTCGGGG 
ACCTCCCAGG AAGAGCTGCG CATCGTGGAG GGTCAGGGCC AGGGTGCAGA CACCGGGCCC 
TCGGCCGACG AGGTCAACAA CAACACCTGC TCAGCCGTGC CCCCGGAAGG CCTCCTCCTG 
GACAGCCCCG AGAAGGCCGC CACAGATGGG CCTCTGGACG CCGCCCTGGA CCACCTGCGC 
CTGGGCAGCA CCTTCACCTT CCGCGTGACT GTCCTGCAGG CGTCCAGCAT CTCCGCCGAA 
TACGCCGACA TTTTCTGCCA GTTCAACTTC ATCCACCGCC ACGACGAGGC CTTCTCCACG 
GAGCCCCTCA AGAACACAGG GAGGGGCCCC CCGCTTGGCT TCTACCACGT CCAGAACATC 
GCAGTGGAGG TGACCAAGTC CTTCATCGAG TACATCAAGA GCCAGCCCAT CGTGTTCGAG 
GTCTTCGGCC ACTACCAGCA GCACCCGTTC CCGCCCCTCT GCAAGGATGT GCTCAGCCCC 
CTGAGGCCCT CGCGCCGCCA CTTCCCGAGG GTCATGCCGC TGTCCAAGCC AGTGCCTGCC 
ACCAAGCTCA GCACGCTAGC GCGACCCTGC CCTGGGCCCT GCCACTGCAA GTACGACCTG 
CTGGTCTACT TTGAGATCTG TGAGCTGGAG GCCAACGGCG ATTACATTCC CGCAGTGGTG 
GACCACCGTG GCGGGATGCC GTGCATGGGG ACCTTCCTGC TGCACCAGGG CATCCAGAGA 
CGGATCACCG TGACGCTGCT GCACGAGACG GGCAGCCACA TCCGCTGGAA GGAAGTTCGA 
GAGCTGGTCG TGGGTCGGAT CCGAAACACT CCCGAAACCG ATGAGTCCCT GATCGACCCC 
AACATCTTGT CGCTCAACAT CCTCTCCTCC GATTATATCC ACCCGGCCCA GGACGACCGG 
ACCTTCTACC AATTCGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCTCT TCTGCTCAAC 
CGGGTCACCC CGTACCGAGA GAAGATCTAC ATGACTCTCT CAGCTTACAT TGAGATGGAG 
AACTGCACTC AGCCGGCCGT CATCACCAAG GACTTCTGCA TGGTCTTCTA CTCTCGGGAC 
GCCAAGCTGC CCGCTTCCCG CTCCATCCGC AACCTGTTTG GCAGCGGGAG CCTCCGGGCC 
TCGGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTCAGCC TGTGCCATGT GGCCGACGCC 
GGCAGCCCAG GGATGCAGCG GCGGCGGCGG CGCGTGCTGG ATACATCCGT GGCCTACGTG 
CGGGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGGAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTTG AGAAGACCAG ACACTACCTT 
CTGCTGCGGG AGAAGCTGGA GACCACCCAG CGGCCCGGCC CTGAGGCGCC GTCCCCCGCC 
TCCAGCGAGG ACTTGGGGTC CCATGGCTCC TCCAGCCCCT CGTCCCCGCT CTCAGCTGAG 
GGCCGCCCGT CGCCTCTGGA GACTCCCAAT GAGAGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGCCTCC TCACGCACAC GTTCAACCGA GAGTACACCC ACAGCCACGT CTGCATCAGT 
GCCAGCGAGA GCAAGCTCTC CGAGATGTCT GTCACCCTGC TTCGGGACCC ATCCATGTCC 
CCGCTGGGGG CGGCCACCCT CACCCCCTCC TCCACCTGCC CCTCTCTGGT CGAAGGGCGG 
TACGGAGCCG CTGAGCTGAG GACCCCCCAG CCCTGCTCCC GGCCCGCCAG CCCAGAGCCT 
GAGCCTGTGC CAGAGGCAGA TTCCAAGAAG CTTCCCTCCC CTGCTCGGGC TGCGGAGGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTTCCC GACATCCAGG AGATCCGGGT CAGCCCCATC 
GTGTCTAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TACGGCGGCC CTACGCCTAC CTGTACAACA GCGACAAGGA CTCCGTGGAG 
AGGTTCGTGC TCAACCTGTC CACCGCGCAG GTGGAGTACA GCGAGGACCA GCAGGCCATG 
CTCAAGACGC CCAACACGTT CGCCGTGTGC ACGGAGCACC GCGGCATCCT GCTGCAAGCC 
AACAGCGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCGCTCCT GGCCGGGACC 
ATACGGTCCA AGCTCTCCAG AAGGCGGTCT GCCCAGATGA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107684.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963159.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

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CloneID	OCe43168
Clone ID Related Accession (Same CDS sequence)	XM_014107684.1
Accession Version	XM_014107684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGCAGTTTCT TGATTCGGAC 
ATGCCTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963159.1
CDS	194..5590
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107684.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107684.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA CCAACACTGT GCCCGGAGGA 
CCCAAATTGA CCAACGCCCT GGTGGGCATG AGCCCCTCGT CCTCGCTCTC GGCCCTGTCC 
AGCCGCGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACGGAG AAGATCATAG CCGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAGGCCCT GCTGGCCGAG 
ATGGGCGTGG CCATGAGGGA GGATGGCGGC ACGCTGGGGG TGTTTTCTCC CAAAAAGACA 
CCACATCTTG TCAACCTGAA TGAGGACCCC TTGATGTCCG AGTGTCTCCT GTACTACATC 
AAGGATGGCA TCACCAGAGT GGGCCGGGAG GATGCGGAGC GGCGGCAGGA CATCGTCCTG 
AGTGGGCACT TCATCAAGGA GGAGCACTGC GTCTTCCGGA GTGATTCCCG AGGGGGCAGT 
GAAGCTGTGG TGACCCTGGA GCCCTGCGAG GGCGCAGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CTAGCATCCT GCGGTCAGGC AACCGCATCA TCATGGGAAA GAGCCACGTG 
TTCCGGTTCA ACCACCCCGA GCAGGCGAGG CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCCGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGTG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGC 
GAGCGGGAGG AGGCCACGTA CCTGCTGGAG CAGCAGAGGC TGGACTACGA GAGCAAGCTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTACCCTG AGGTGAACGA GGAGGAGGAA 
GAGCCGGAGG ACGAAGTCCA GTGGACGGAG AGGGAGTGTG AGCTGGCGCT CTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACGTCG CTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AAGCCAACGC CATTAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CCGACACGCT CTACTCCCCG CTGCCGCCGG ACCTGCTGCC CCCAGAGGCC 
GCCAAAGACC GGGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAGC TCAGGCAGCG CCTGGACCTG 
ATGCGGGAGA TGTACGACCG GGCGGCAGAG GTGCCCTCCA GTGTCATCGA GGACTGTGAC 
AACGTGGTGA CGGGCGGAGA CCCCTTCTAC GACCGCTTTC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCA TCTCTGGCTG CAACAGCTAC CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGGAGG ACGACGTCTT TCCGGAGCAC GCGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TCTACCTGAG CAACCTGCTG 
TACCCTGTCC CCCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGAGA GGTGAAGGGC 
TTCCTCCGCG TGGCCGTGCA GGCCACCTCA GCCGACGAAG AGGCCCCCGA CTATGGCTCT 
GGCGTCCGAC AGTCCGGCAC GGCCAGAATC TCCTTTGACG ACCAGCATTT TGAAAAGTTC 
CAGTCTGAGT CCTGCCCTGT GGTGGGGATG TCCCGCTCGG GGACCTCCCA GGAAGAGCTG 
CGCATCGTGG AGGGTCAGGG CCAGGGTGCA GACACCGGGC CCTCGGCCGA CGAGGTCAAC 
AACAACACCT GCTCAGCCGT GCCCCCGGAA GGCCTCCTCC TGGACAGCCC CGAGAAGGCC 
GCCACAGATG GGCCTCTGGA CGCCGCCCTG GACCACCTGC GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CTGTCCTGCA GGCGTCCAGC ATCTCCGCCG AATACGCCGA CATTTTCTGC 
CAGTTCAACT TCATCCACCG CCACGACGAG GCCTTCTCCA CGGAGCCCCT CAAGAACACA 
GGGAGGGGCC CCCCGCTTGG CTTCTACCAC GTCCAGAACA TCGCAGTGGA GGTGACCAAG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC ATCGTGTTCG AGGTCTTCGG CCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAT GTGCTCAGCC CCCTGAGGCC CTCGCGCCGC 
CACTTCCCGA GGGTCATGCC GCTGTCCAAG CCAGTGCCTG CCACCAAGCT CAGCACGCTA 
GCGCGACCCT GCCCTGGGCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTTGAGATC 
TGTGAGCTGG AGGCCAACGG CGATTACATT CCCGCAGTGG TGGACCACCG TGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTGCACCAG GGCATCCAGA GACGGATCAC CGTGACGCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTTC GAGAGCTGGT CGTGGGTCGG 
ATCCGAAACA CTCCCGAAAC CGATGAGTCC CTGATCGACC CCAACATCTT GTCGCTCAAC 
ATCCTCTCCT CCGATTATAT CCACCCGGCC CAGGACGACC GGCAGTTTCT TGATTCGGAC 
ATGCCTAGGA CCTTCTACCA ATTCGAGGCT GCGTGGGACA GCTCCATGCA CAACTCTCTT 
CTGCTCAACC GGGTCACCCC GTACCGAGAG AAGATCTACA TGACTCTCTC AGCTTACATT 
GAGATGGAGA ACTGCACTCA GCCGGCCGTC ATCACCAAGG ACTTCTGCAT GGTCTTCTAC 
TCTCGGGACG CCAAGCTGCC CGCTTCCCGC TCCATCCGCA ACCTGTTTGG CAGCGGGAGC 
CTCCGGGCCT CGGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTCAGCCT GTGCCATGTG 
GCCGACGCCG GCAGCCCAGG GATGCAGCGG CGGCGGCGGC GCGTGCTGGA TACATCCGTG 
GCCTACGTGC GGGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGGAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTTGA GAAGACCAGA 
CACTACCTTC TGCTGCGGGA GAAGCTGGAG ACCACCCAGC GGCCCGGCCC TGAGGCGCCG 
TCCCCCGCCT CCAGCGAGGA CTTGGGGTCC CATGGCTCCT CCAGCCCCTC GTCCCCGCTC 
TCAGCTGAGG GCCGCCCGTC GCCTCTGGAG ACTCCCAATG AGAGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGCCTCCT CACGCACACG TTCAACCGAG AGTACACCCA CAGCCACGTC 
TGCATCAGTG CCAGCGAGAG CAAGCTCTCC GAGATGTCTG TCACCCTGCT TCGGGACCCA 
TCCATGTCCC CGCTGGGGGC GGCCACCCTC ACCCCCTCCT CCACCTGCCC CTCTCTGGTC 
GAAGGGCGGT ACGGAGCCGC TGAGCTGAGG ACCCCCCAGC CCTGCTCCCG GCCCGCCAGC 
CCAGAGCCTG AGCCTGTGCC AGAGGCAGAT TCCAAGAAGC TTCCCTCCCC TGCTCGGGCT 
GCGGAGGCCG ACAAGGAGCC CCAGCGCCTG CTGGTTCCCG ACATCCAGGA GATCCGGGTC 
AGCCCCATCG TGTCTAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT ACGGCGGCCC TACGCCTACC TGTACAACAG CGACAAGGAC 
TCCGTGGAGA GGTTCGTGCT CAACCTGTCC ACCGCGCAGG TGGAGTACAG CGAGGACCAG 
CAGGCCATGC TCAAGACGCC CAACACGTTC GCCGTGTGCA CGGAGCACCG CGGCATCCTG 
CTGCAAGCCA ACAGCGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCGCTCCTG 
GCCGGGACCA TACGGTCCAA GCTCTCCAGA AGGCGGTCTG CCCAGATGAG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107685.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963160.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

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CloneID	OCe43169
Clone ID Related Accession (Same CDS sequence)	XM_014107685.1
Accession Version	XM_014107685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCATCTCTGG CTGCAACAGC 
TACCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGG AGGACGACGT CTTTCCGGAG 
CACGCGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTCTACCT GAGCAACCTG CTGTACCCTG TCCCCCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG GGCTTCCTCC GCGTGGCCGT GCAGGCCACC 
TCAGCCGACG AAGAGGCCCC CGACTATGGC TCTGGCGTCC GACAGTCCGG CACGGCCAGA 
ATCTCCTTTG ACGACCAGCA TTTTGAAAAG TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG 
ATGTCCCGCT CGGGGACCTC CCAGGAAGAG CTGCGCATCG TGGAGGGTCA GGGCCAGGGT 
GCAGACACCG GGCCCTCGGC CGACGAGGTC AACAACAACA CCTGCTCAGC CGTGCCCCCG 
GAAGGCCTCC TCCTGGACAG CCCCGAGAAG GCCGCCACAG ATGGGCCTCT GGACGCCGCC 
CTGGACCACC TGCGCCTGGG CAGCACCTTC ACCTTCCGCG TGACTGTCCT GCAGGCGTCC 
AGCATCTCCG CCGAATACGC CGACATTTTC TGCCAGTTCA ACTTCATCCA CCGCCACGAC 
GAGGCCTTCT CCACGGAGCC CCTCAAGAAC ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC 
CACGTCCAGA ACATCGCAGT GGAGGTGACC AAGTCCTTCA TCGAGTACAT CAAGAGCCAG 
CCCATCGTGT TCGAGGTCTT CGGCCACTAC CAGCAGCACC CGTTCCCGCC CCTCTGCAAG 
GATGTGCTCA GCCCCCTGAG GCCCTCGCGC CGCCACTTCC CGAGGGTCAT GCCGCTGTCC 
AAGCCAGTGC CTGCCACCAA GCTCAGCACG CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC 
TGCAAGTACG ACCTGCTGGT CTACTTTGAG ATCTGTGAGC TGGAGGCCAA CGGCGATTAC 
ATTCCCGCAG TGGTGGACCA CCGTGGCGGG ATGCCGTGCA TGGGGACCTT CCTGCTGCAC 
CAGGGCATCC AGAGACGGAT CACCGTGACG CTGCTGCACG AGACGGGCAG CCACATCCGC 
TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT CGGATCCGAA ACACTCCCGA AACCGATGAG 
TCCCTGATCG ACCCCAACAT CTTGTCGCTC AACATCCTCT CCTCCGATTA TATCCACCCG 
GCCCAGGACG ACCGGCAGTT TCTTGATTCG GACATGCCTA GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963160.1
CDS	194..5563
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107685.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107685.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCATCTCTGG CTGCAACAGC 
TACCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGG AGGACGACGT CTTTCCGGAG 
CACGCGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTCTACCT GAGCAACCTG CTGTACCCTG TCCCCCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG AGAGGTGAAG GGCTTCCTCC GCGTGGCCGT GCAGGCCACC 
TCAGCCGACG AAGAGGCCCC CGACTATGGC TCTGGCGTCC GACAGTCCGG CACGGCCAGA 
ATCTCCTTTG ACGACCAGCA TTTTGAAAAG TTCCAGTCTG AGTCCTGCCC TGTGGTGGGG 
ATGTCCCGCT CGGGGACCTC CCAGGAAGAG CTGCGCATCG TGGAGGGTCA GGGCCAGGGT 
GCAGACACCG GGCCCTCGGC CGACGAGGTC AACAACAACA CCTGCTCAGC CGTGCCCCCG 
GAAGGCCTCC TCCTGGACAG CCCCGAGAAG GCCGCCACAG ATGGGCCTCT GGACGCCGCC 
CTGGACCACC TGCGCCTGGG CAGCACCTTC ACCTTCCGCG TGACTGTCCT GCAGGCGTCC 
AGCATCTCCG CCGAATACGC CGACATTTTC TGCCAGTTCA ACTTCATCCA CCGCCACGAC 
GAGGCCTTCT CCACGGAGCC CCTCAAGAAC ACAGGGAGGG GCCCCCCGCT TGGCTTCTAC 
CACGTCCAGA ACATCGCAGT GGAGGTGACC AAGTCCTTCA TCGAGTACAT CAAGAGCCAG 
CCCATCGTGT TCGAGGTCTT CGGCCACTAC CAGCAGCACC CGTTCCCGCC CCTCTGCAAG 
GATGTGCTCA GCCCCCTGAG GCCCTCGCGC CGCCACTTCC CGAGGGTCAT GCCGCTGTCC 
AAGCCAGTGC CTGCCACCAA GCTCAGCACG CTAGCGCGAC CCTGCCCTGG GCCCTGCCAC 
TGCAAGTACG ACCTGCTGGT CTACTTTGAG ATCTGTGAGC TGGAGGCCAA CGGCGATTAC 
ATTCCCGCAG TGGTGGACCA CCGTGGCGGG ATGCCGTGCA TGGGGACCTT CCTGCTGCAC 
CAGGGCATCC AGAGACGGAT CACCGTGACG CTGCTGCACG AGACGGGCAG CCACATCCGC 
TGGAAGGAAG TTCGAGAGCT GGTCGTGGGT CGGATCCGAA ACACTCCCGA AACCGATGAG 
TCCCTGATCG ACCCCAACAT CTTGTCGCTC AACATCCTCT CCTCCGATTA TATCCACCCG 
GCCCAGGACG ACCGGCAGTT TCTTGATTCG GACATGCCTA GGACCTTCTA CCAATTCGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCT CTTCTGCTCA ACCGGGTCAC CCCGTACCGA 
GAGAAGATCT ACATGACTCT CTCAGCTTAC ATTGAGATGG AGAACTGCAC TCAGCCGGCC 
GTCATCACCA AGGACTTCTG CATGGTCTTC TACTCTCGGG ACGCCAAGCT GCCCGCTTCC 
CGCTCCATCC GCAACCTGTT TGGCAGCGGG AGCCTCCGGG CCTCGGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTCAG CCTGTGCCAT GTGGCCGACG CCGGCAGCCC AGGGATGCAG 
CGGCGGCGGC GGCGCGTGCT GGATACATCC GTGGCCTACG TGCGGGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGGAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT TGAGAAGACC AGACACTACC TTCTGCTGCG GGAGAAGCTG 
GAGACCACCC AGCGGCCCGG CCCTGAGGCG CCGTCCCCCG CCTCCAGCGA GGACTTGGGG 
TCCCATGGCT CCTCCAGCCC CTCGTCCCCG CTCTCAGCTG AGGGCCGCCC GTCGCCTCTG 
GAGACTCCCA ATGAGAGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGCCT CCTCACGCAC 
ACGTTCAACC GAGAGTACAC CCACAGCCAC GTCTGCATCA GTGCCAGCGA GAGCAAGCTC 
TCCGAGATGT CTGTCACCCT GCTTCGGGAC CCATCCATGT CCCCGCTGGG GGCGGCCACC 
CTCACCCCCT CCTCCACCTG CCCCTCTCTG GTCGAAGGGC GGTACGGAGC CGCTGAGCTG 
AGGACCCCCC AGCCCTGCTC CCGGCCCGCC AGCCCAGAGC CTGAGCCTGT GCCAGAGGCA 
GATTCCAAGA AGCTTCCCTC CCCTGCTCGG GCTGCGGAGG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTTC CCGACATCCA GGAGATCCGG GTCAGCCCCA TCGTGTCTAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTACGGCGG 
CCCTACGCCT ACCTGTACAA CAGCGACAAG GACTCCGTGG AGAGGTTCGT GCTCAACCTG 
TCCACCGCGC AGGTGGAGTA CAGCGAGGAC CAGCAGGCCA TGCTCAAGAC GCCCAACACG 
TTCGCCGTGT GCACGGAGCA CCGCGGCATC CTGCTGCAAG CCAACAGCGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCGCTC CTGGCCGGGA CCATACGGTC CAAGCTCTCC 
AGAAGGCGGT CTGCCCAGAT GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107686.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963161.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

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CloneID	OCe43170
Clone ID Related Accession (Same CDS sequence)	XM_014107686.1
Accession Version	XM_014107686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GCAGTTTCTT 
GATTCGGACA TGCCTAGGAC CTTCTACCAA TTCGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCTCTTC TGCTCAACCG GGTCACCCCG TACCGAGAGA AGATCTACAT GACTCTCTCA 
GCTTACATTG AGATGGAGAA CTGCACTCAG CCGGCCGTCA TCACCAAGGA CTTCTGCATG 
GTCTTCTACT CTCGGGACGC CAAGCTGCCC GCTTCCCGCT CCATCCGCAA CCTGTTTGGC 
AGCGGGAGCC TCCGGGCCTC GGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTCAGCCTG 
TGCCATGTGG CCGACGCCGG CAGCCCAGGG ATGCAGCGGC GGCGGCGGCG CGTGCTGGAT 
ACATCCGTGG CCTACGTGCG GGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGGAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTTGAG 
AAGACCAGAC ACTACCTTCT GCTGCGGGAG AAGCTGGAGA CCACCCAGCG GCCCGGCCCT 
GAGGCGCCGT CCCCCGCCTC CAGCGAGGAC TTGGGGTCCC ATGGCTCCTC CAGCCCCTCG 
TCCCCGCTCT CAGCTGAGGG CCGCCCGTCG CCTCTGGAGA CTCCCAATGA GAGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGCCTCCTC ACGCACACGT TCAACCGAGA GTACACCCAC 
AGCCACGTCT GCATCAGTGC CAGCGAGAGC AAGCTCTCCG AGATGTCTGT CACCCTGCTT 
CGGGACCCAT CCATGTCCCC GCTGGGGGCG GCCACCCTCA CCCCCTCCTC CACCTGCCCC 
TCTCTGGTCG AAGGGCGGTA CGGAGCCGCT GAGCTGAGGA CCCCCCAGCC CTGCTCCCGG 
CCCGCCAGCC CAGAGCCTGA GCCTGTGCCA GAGGCAGATT CCAAGAAGCT TCCCTCCCCT 
GCTCGGGCTG CGGAGGCCGA CAAGGAGCCC CAGCGCCTGC TGGTTCCCGA CATCCAGGAG 
ATCCGGGTCA GCCCCATCGT GTCTAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTA CGGCGGCCCT ACGCCTACCT GTACAACAGC 
GACAAGGACT CCGTGGAGAG GTTCGTGCTC AACCTGTCCA CCGCGCAGGT GGAGTACAGC 
GAGGACCAGC AGGCCATGCT CAAGACGCCC AACACGTTCG CCGTGTGCAC GGAGCACCGC 
GGCATCCTGC TGCAAGCCAA CAGCGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCGCTCCTGG CCGGGACCAT ACGGTCCAAG CTCTCCAGAA GGCGGTCTGC CCAGATGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963161.1
CDS	194..5539
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107686.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107686.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GCAGTTTCTT 
GATTCGGACA TGCCTAGGAC CTTCTACCAA TTCGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCTCTTC TGCTCAACCG GGTCACCCCG TACCGAGAGA AGATCTACAT GACTCTCTCA 
GCTTACATTG AGATGGAGAA CTGCACTCAG CCGGCCGTCA TCACCAAGGA CTTCTGCATG 
GTCTTCTACT CTCGGGACGC CAAGCTGCCC GCTTCCCGCT CCATCCGCAA CCTGTTTGGC 
AGCGGGAGCC TCCGGGCCTC GGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTCAGCCTG 
TGCCATGTGG CCGACGCCGG CAGCCCAGGG ATGCAGCGGC GGCGGCGGCG CGTGCTGGAT 
ACATCCGTGG CCTACGTGCG GGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGGAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTTGAG 
AAGACCAGAC ACTACCTTCT GCTGCGGGAG AAGCTGGAGA CCACCCAGCG GCCCGGCCCT 
GAGGCGCCGT CCCCCGCCTC CAGCGAGGAC TTGGGGTCCC ATGGCTCCTC CAGCCCCTCG 
TCCCCGCTCT CAGCTGAGGG CCGCCCGTCG CCTCTGGAGA CTCCCAATGA GAGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGCCTCCTC ACGCACACGT TCAACCGAGA GTACACCCAC 
AGCCACGTCT GCATCAGTGC CAGCGAGAGC AAGCTCTCCG AGATGTCTGT CACCCTGCTT 
CGGGACCCAT CCATGTCCCC GCTGGGGGCG GCCACCCTCA CCCCCTCCTC CACCTGCCCC 
TCTCTGGTCG AAGGGCGGTA CGGAGCCGCT GAGCTGAGGA CCCCCCAGCC CTGCTCCCGG 
CCCGCCAGCC CAGAGCCTGA GCCTGTGCCA GAGGCAGATT CCAAGAAGCT TCCCTCCCCT 
GCTCGGGCTG CGGAGGCCGA CAAGGAGCCC CAGCGCCTGC TGGTTCCCGA CATCCAGGAG 
ATCCGGGTCA GCCCCATCGT GTCTAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTA CGGCGGCCCT ACGCCTACCT GTACAACAGC 
GACAAGGACT CCGTGGAGAG GTTCGTGCTC AACCTGTCCA CCGCGCAGGT GGAGTACAGC 
GAGGACCAGC AGGCCATGCT CAAGACGCCC AACACGTTCG CCGTGTGCAC GGAGCACCGC 
GGCATCCTGC TGCAAGCCAA CAGCGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCGCTCCTGG CCGGGACCAT ACGGTCCAAG CTCTCCAGAA GGCGGTCTGC CCAGATGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107688.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963163.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

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CloneID	OCe43172
Clone ID Related Accession (Same CDS sequence)	XM_014107688.1 , XM_014107688.2
Accession Version	XM_014107688.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	kinesin-like protein KIF1A isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963163.1
CDS	194..5512
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107688.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107688.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_014107688.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963163.1 Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X17, mRNA.

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CloneID	OCe43172
Clone ID Related Accession (Same CDS sequence)	XM_014107688.1 , XM_014107688.2
Accession Version	XM_014107688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	kinesin-like protein KIF1A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963163.1
CDS	207..5525
Translation	MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQATSADEEAPDYGSGVRQSGTARISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADTGPSADEVNNNTCSAVPPEGLLLDSPEKAATDGPLDAALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLARPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLNILSSDYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLETTQRPGPEAPSPASSEDLGSHGSSSPSSPLSAEGRPSPLETPNERQRELAVKCLRLLTHTFNREYTHSHVCISASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGAAELRTPQPCSRPASPEPEPVPEADSKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYLYNSDKDSVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_014107688.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinesin family member 1A (KIF1A), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107688.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAATTC CCGAGAGATG 
AGCCGAGACT CCAAGTGCAT CATCCAGATG TCCGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACACC CAAGAGCTTC AGCTTCGACT ACTCCTACTG GTCACACACC 
TCGCCCGAGG ACATCAACTA CGCGTCGCAG AAGCAGGTGT ACCGCGACAT CGGCGAGGAG 
ATGCTGCAGC ACGCCTTCGA GGGCTACAAC GTGTGCATCT TCGCCTACGG GCAGACCGGG 
GCCGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCG 
CAGCTCTGCG AGGACCTGTT TTCCCGTATC AATGACACGA CCAACGACAA CATGTCCTAC 
TCTGTAGAGG TGAGCTACAT GGAGATTTAC TGTGAGCGAG TCCGTGACCT CCTGAACCCC 
AAGAACAAGG GGAATCTGCG CGTGCGAGAG CACCCGCTGC TGGGGCCCTA CGTGGAGGAC 
CTCTCCAAGC TGGCCGTCAC CTCCTACAAT GACATCCAGG ACCTCATGGA CTCAGGGAAC 
AAGGCCAGGA CCGTGGCCGC CACGAACATG AATGAAACCA GTAGCCGCTC CCACGCCGTC 
TTCAACATCA TCTTCACCCA GAAGCGCCAC GACGCGGAGA CCAACATCAC CACGGAGAAG 
GTGAGCAAGA TCAGCCTGGT GGACCTGGCG GGCAGCGAGC GGGCCGACTC CACGGGCGCC 
AAGGGCACGC GTCTCAAGGA GGGCGCCAAC ATCAACAAGT CCTTGACCAC GCTGGGGAAG 
GTCATCTCCG CTCTGGCCGA GATGGACTCC GGGCCCAACA AGAACAAGAA AAAGAAGAAG 
ACCGATTTCA TTCCCTACAG AGACTCCGTG CTGACCTGGC TCCTCCGGGA AAATCTGGGC 
GGGAACTCGA GGACGGCGAT GGTGGCGGCC CTGAGCCCCG CGGACATCAA CTACGACGAG 
ACGCTGAGCA CGCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGTAA CGCCGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGT GAGCTCAAGG ACGAGGTGAC CCGGCTGCGG 
GACCTGCTGT ACGCCCAGGG TCTCGGGGAC ATCACCGACA TGACCAACGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCGCT CTCGGCCCTG TCCAGCCGCG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACG 
GAGAAGATCA TAGCCGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAGGC CCTGCTGGCC GAGATGGGCG TGGCCATGAG GGAGGATGGC 
GGCACGCTGG GGGTGTTTTC TCCCAAAAAG ACACCACATC TTGTCAACCT GAATGAGGAC 
CCCTTGATGT CCGAGTGTCT CCTGTACTAC ATCAAGGATG GCATCACCAG AGTGGGCCGG 
GAGGATGCGG AGCGGCGGCA GGACATCGTC CTGAGTGGGC ACTTCATCAA GGAGGAGCAC 
TGCGTCTTCC GGAGTGATTC CCGAGGGGGC AGTGAAGCTG TGGTGACCCT GGAGCCCTGC 
GAGGGCGCAG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCTAGCAT CCTGCGGTCA 
GGCAACCGCA TCATCATGGG AAAGAGCCAC GTGTTCCGGT TCAACCACCC CGAGCAGGCG 
AGGCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCCG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GTGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGCGAGCGGG AGGAGGCCAC GTACCTGCTG 
GAGCAGCAGA GGCTGGACTA CGAGAGCAAG CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTACC CTGAGGTGAA CGAGGAGGAG GAAGAGCCGG AGGACGAAGT CCAGTGGACG 
GAGAGGGAGT GTGAGCTGGC GCTCTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACG 
TCGCTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAAGCCAA CGCCATTAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACCGACAC GCTCTACTCC 
CCGCTGCCGC CGGACCTGCT GCCCCCAGAG GCCGCCAAAG ACCGGGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AGCTCAGGCA GCGCCTGGAC CTGATGCGGG AGATGTACGA CCGGGCGGCA 
GAGGTGCCCT CCAGTGTCAT CGAGGACTGT GACAACGTGG TGACGGGCGG AGACCCCTTC 
TACGACCGCT TTCCCTGGTT CCGGCTGGTG GGCAGCTACC CTCTTCTCAA CACATGCATG 
AGCGAGCGCA TGGCTGCTCT CACCCCCTCC CCCACCTTCT CGAGCCCCGA CTCCGACGCC 
ACCGAGCCTG CCGAGGAGCA GAGCGTGGGG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG ACCTGGAGGA CGACGTCTTT CCGGAGCACG CGCTGTGCGA CGGCCGGGAC 
CCGTTTTACG ACCGGCCCCC CCTGTTCAGT TTAGTAGGAA GGGCCTTCGT CTACCTGAGC 
AACCTGCTGT ACCCTGTCCC CCTGGTGCAC CGCGTGGCCG TCGTCAGCGA GAAGGGAGAG 
GTGAAGGGCT TCCTCCGCGT GGCCGTGCAG GCCACCTCAG CCGACGAAGA GGCCCCCGAC 
TATGGCTCTG GCGTCCGACA GTCCGGCACG GCCAGAATCT CCTTTGACGA CCAGCATTTT 
GAAAAGTTCC AGTCTGAGTC CTGCCCTGTG GTGGGGATGT CCCGCTCGGG GACCTCCCAG 
GAAGAGCTGC GCATCGTGGA GGGTCAGGGC CAGGGTGCAG ACACCGGGCC CTCGGCCGAC 
GAGGTCAACA ACAACACCTG CTCAGCCGTG CCCCCGGAAG GCCTCCTCCT GGACAGCCCC 
GAGAAGGCCG CCACAGATGG GCCTCTGGAC GCCGCCCTGG ACCACCTGCG CCTGGGCAGC 
ACCTTCACCT TCCGCGTGAC TGTCCTGCAG GCGTCCAGCA TCTCCGCCGA ATACGCCGAC 
ATTTTCTGCC AGTTCAACTT CATCCACCGC CACGACGAGG CCTTCTCCAC GGAGCCCCTC 
AAGAACACAG GGAGGGGCCC CCCGCTTGGC TTCTACCACG TCCAGAACAT CGCAGTGGAG 
GTGACCAAGT CCTTCATCGA GTACATCAAG AGCCAGCCCA TCGTGTTCGA GGTCTTCGGC 
CACTACCAGC AGCACCCGTT CCCGCCCCTC TGCAAGGATG TGCTCAGCCC CCTGAGGCCC 
TCGCGCCGCC ACTTCCCGAG GGTCATGCCG CTGTCCAAGC CAGTGCCTGC CACCAAGCTC 
AGCACGCTAG CGCGACCCTG CCCTGGGCCC TGCCACTGCA AGTACGACCT GCTGGTCTAC 
TTTGAGATCT GTGAGCTGGA GGCCAACGGC GATTACATTC CCGCAGTGGT GGACCACCGT 
GGCGGGATGC CGTGCATGGG GACCTTCCTG CTGCACCAGG GCATCCAGAG ACGGATCACC 
GTGACGCTGC TGCACGAGAC GGGCAGCCAC ATCCGCTGGA AGGAAGTTCG AGAGCTGGTC 
GTGGGTCGGA TCCGAAACAC TCCCGAAACC GATGAGTCCC TGATCGACCC CAACATCTTG 
TCGCTCAACA TCCTCTCCTC CGATTATATC CACCCGGCCC AGGACGACCG GACCTTCTAC 
CAATTCGAGG CTGCGTGGGA CAGCTCCATG CACAACTCTC TTCTGCTCAA CCGGGTCACC 
CCGTACCGAG AGAAGATCTA CATGACTCTC TCAGCTTACA TTGAGATGGA GAACTGCACT 
CAGCCGGCCG TCATCACCAA GGACTTCTGC ATGGTCTTCT ACTCTCGGGA CGCCAAGCTG 
CCCGCTTCCC GCTCCATCCG CAACCTGTTT GGCAGCGGGA GCCTCCGGGC CTCGGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTCAGC CTGTGCCATG TGGCCGACGC CGGCAGCCCA 
GGGATGCAGC GGCGGCGGCG GCGCGTGCTG GATACATCCG TGGCCTACGT GCGGGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGGAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTT GAGAAGACCA GACACTACCT TCTGCTGCGG 
GAGAAGCTGG AGACCACCCA GCGGCCCGGC CCTGAGGCGC CGTCCCCCGC CTCCAGCGAG 
GACTTGGGGT CCCATGGCTC CTCCAGCCCC TCGTCCCCGC TCTCAGCTGA GGGCCGCCCG 
TCGCCTCTGG AGACTCCCAA TGAGAGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGCCTC 
CTCACGCACA CGTTCAACCG AGAGTACACC CACAGCCACG TCTGCATCAG TGCCAGCGAG 
AGCAAGCTCT CCGAGATGTC TGTCACCCTG CTTCGGGACC CATCCATGTC CCCGCTGGGG 
GCGGCCACCC TCACCCCCTC CTCCACCTGC CCCTCTCTGG TCGAAGGGCG GTACGGAGCC 
GCTGAGCTGA GGACCCCCCA GCCCTGCTCC CGGCCCGCCA GCCCAGAGCC TGAGCCTGTG 
CCAGAGGCAG ATTCCAAGAA GCTTCCCTCC CCTGCTCGGG CTGCGGAGGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTTCC CGACATCCAG GAGATCCGGG TCAGCCCCAT CGTGTCTAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTACGGCGGC CCTACGCCTA CCTGTACAAC AGCGACAAGG ACTCCGTGGA GAGGTTCGTG 
CTCAACCTGT CCACCGCGCA GGTGGAGTAC AGCGAGGACC AGCAGGCCAT GCTCAAGACG 
CCCAACACGT TCGCCGTGTG CACGGAGCAC CGCGGCATCC TGCTGCAAGC CAACAGCGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCGCTCC TGGCCGGGAC CATACGGTCC 
AAGCTCTCCA GAAGGCGGTC TGCCCAGATG AGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KIF1A
Entrez Gene ID	486202
Full Name	kinesin family member 1A
General protein information	Preferred Names kinesin family member 1A Names kinesin-like protein KIF1A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	KIF1A	XP_002799156.1
Gallus gallus (chicken)	KIF1A	XP_003641781.1
Rattus norvegicus (Norway rat)	Kif1a	XP_006245623.1
Drosophila melanogaster (fruit fly)	unc-104	NP_001246373.1
Anopheles gambiae (African malaria mosquito)	KIF1A_ANOGA	XP_314493.3
Pan troglodytes (chimpanzee)	KIF1A	XP_003309612.1
Bos taurus (cattle)	KIF1A	XP_005205116.1
Mus musculus (house mouse)	Kif1a	NP_032466.2
Caenorhabditis elegans (roundworm)	unc-104	NP_001022041.1
Homo sapiens (human)	KIF1A	NP_004312.2
Canis lupus familiaris (dog)	KIF1A	NP_001161945.1
Danio rerio (zebrafish)	kif1a	XP_005166002.1
Danio rerio (zebrafish)	kif1ab	XP_005171271.1
Xenopus tropicalis (tropical clawed frog)	kif1a	XP_002933380.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIF1A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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